JP2013021981A - Light irradiation type culture apparatus - Google Patents

Light irradiation type culture apparatus Download PDF

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JP2013021981A
JP2013021981A JP2011160474A JP2011160474A JP2013021981A JP 2013021981 A JP2013021981 A JP 2013021981A JP 2011160474 A JP2011160474 A JP 2011160474A JP 2011160474 A JP2011160474 A JP 2011160474A JP 2013021981 A JP2013021981 A JP 2013021981A
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light
culture
partition
culture solution
cylinder member
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Mie Mori
美栄 森
Hiroshi Tanaka
浩 田中
Kosuke Ishii
浩介 石井
Katsuaki Matsuzawa
克明 松澤
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IHI Corp
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IHI Corp
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Abstract

PROBLEM TO BE SOLVED: To promote photosynthesis irrespective of turbidity of a culture solution.SOLUTION: A partition cylinder 3 for an air lift having the internal space 14 between an inner cylindrical member 10 and an outer cylindrical member 11 is arranged in the central part of a culture tank 1. A fluorescent lamp 18 to be a light source is arranged in the internal space 14 of the partition cylinder 3. Convex portions 10a, 11a for functioning the surrounding wall as a plane-convex lens and concave portions 10b, 11b for functioning the surrounding wall as a plane-concave lens are alternately arranged in the vertical direction in the inner cylindrical member 10 and the outer cylindrical member 11. In inside and outer peripheral side regions of the partition cylinder 3, bright sections for condensing rays of the fluorescent lamp 18 at the parts where the convex portions 10a, 11a are made on the inner cylindrical member 10 and the outer cylindrical member 11 and dark sections are alternately formed. The photosynthesis is promoted owing to a flashing light effect by bringing microalgae moving with a circulation flow of the culture solution 2 formed in the culture tank 1 to pass through the bright section and the dark section one by one.

Description

本発明は、微細藻類等の光合成生物を培養するために用いる光照射型培養装置に関するものである。   The present invention relates to a light irradiation type culture apparatus used for culturing photosynthetic organisms such as microalgae.

微細藻類等の光合成生物を培養液中に懸濁した状態で培養を行う培養手法の1つとして、エアリフト型の培養槽を用いることが知られている(たとえば、特許文献1参照)。   As one of the culture methods for culturing photosynthetic organisms such as microalgae suspended in a culture solution, it is known to use an airlift type culture tank (see, for example, Patent Document 1).

又、藻類等の光合成生物を培養液中に懸濁した状態で培養を行う際に、照明光の明暗を交互に与えることで、フラッシングライト効果(光合成の明反応、暗反応サイクル効果)により光合成の効率を高めて、上記光合成生物の増殖の促進化を図るようにすることは、従来提案されている(たとえば、特許文献2参照)。   In addition, when cultivating photosynthetic organisms such as algae suspended in a culture solution, photosynthesis is performed by the flashing light effect (light reaction of light synthesis, dark reaction cycle effect) by alternately giving the brightness of illumination light. Conventionally, it has been proposed to increase the efficiency of the photosynthesis and promote the growth of the photosynthetic organism (see, for example, Patent Document 2).

特表2002−541788号公報、図2JP-T-2002-541788, FIG. 特開2007−43909号公報JP 2007-43909 A

ところが、上記特許文献1に示されたような従来のエアリフト型の培養槽は、通常、該培養槽の周囲に配置した外部の光源が発する光を、上記培養槽の光透過性を有する周壁を単に透過させて該培養槽内の光合成生物が懸濁された培養液に対して照射させるようにしてある。そのため、上記光源からの光は、培養時間の経過に伴って上記培養液の濁度が増加してきたときに、該培養液を透過しにくくなる。   However, the conventional air-lift type culture tank as shown in Patent Document 1 usually has a peripheral wall having light permeability of the culture tank that emits light emitted from an external light source disposed around the culture tank. The medium is simply permeated to irradiate the culture solution in which the photosynthetic organisms in the culture tank are suspended. Therefore, the light from the light source is less likely to pass through the culture solution when the turbidity of the culture solution increases with the lapse of culture time.

したがって、上記培養液の濁度が増加した状態では、光源からの光は、上記培養槽の周壁近傍の培養液中に含まれている光合成生物にしか当たらない。このため、上記従来のエアリフト型の培養槽は、光エネルギーが効率よく利用されていないというのが実状である。   Therefore, in the state where the turbidity of the culture solution is increased, the light from the light source hits only the photosynthetic organisms contained in the culture solution near the peripheral wall of the culture tank. For this reason, the above-mentioned conventional air lift type culture tank is actually using light energy efficiently.

しかも、上記特許文献1には、光合成生物の光合成をフラッシングライト効果により促進する考えは何ら示されていない。   Moreover, Patent Document 1 does not show any idea of promoting photosynthesis of photosynthetic organisms by the flashing light effect.

一方、上記特許文献2には、光合成生物の光合成をフラッシングライト効果により促進する考えは示されている。   On the other hand, Patent Document 2 discloses the idea of promoting photosynthesis of photosynthetic organisms by the flashing light effect.

しかし、上記特許文献2に示されている上記光合成生物に光の明暗を与える手段は、培養容器内で発生させるゲルトラー渦により、光合成生物が培養容器の周壁近傍の明部と、容器中央部の暗部を交互に移動することで実現されている。そのため、培養初期の培養液のように、懸濁されている光合成生物の量(濃度)が少なくて培養液の濁度が低い場合は、照射される光が培養容器の容器中央部まで容易に到達するため、該培養容器の周壁近傍と、容器中央部での光量の差(明暗の差)が少ない。よって、上記特許文献2に示された手法は、培養液の濁度が低い場合に光合成生物に与える上記フラッシングライト効果が小さくなってしまう。   However, the means for imparting light and darkness to the photosynthetic organism shown in Patent Document 2 is that the photosynthetic organism has a bright portion near the peripheral wall of the culture vessel and a central portion of the vessel due to the geltler vortex generated in the culture vessel This is realized by moving the dark part alternately. Therefore, when the amount (concentration) of suspended photosynthetic organisms is low and the turbidity of the culture solution is low, such as the culture solution at the beginning of the culture, the irradiated light can easily reach the center of the culture vessel. Therefore, the difference in the amount of light (difference in brightness) between the vicinity of the peripheral wall of the culture vessel and the center of the vessel is small. Therefore, the technique disclosed in Patent Document 2 reduces the flashing light effect given to photosynthetic organisms when the turbidity of the culture solution is low.

そこで、本発明は、培養液の濁度にかかわらず、該培養液中に懸濁された光合成生物に効率よくフラッシングライト効果を与えて、該光合成生物の増殖の促進化を図ることができる光照射型培養装置を提供しようとするものである。   Therefore, the present invention provides a light that can efficiently provide a flushing light effect to photosynthetic organisms suspended in the culture solution, regardless of the turbidity of the culture solution, and promote the growth of the photosynthetic organisms. An object is to provide an irradiation type culture apparatus.

本発明は、上記課題を解決するために、請求項1に対応して、光合成生物を懸濁する培養液を貯留する培養槽と、該培養槽の内側に設けた光透過性を有する仕切筒と、上記仕切筒の下端側開口部の下方に設けてガスの気泡を上記培養槽内に貯留した培養液に吹き込むための散気管と、上記培養槽内の培養液を照明するための照明手段を備え、且つ該照明手段は、上記仕切筒の内側の領域及び外周側の領域に、光源からの照明光を集光して照射する明部と、暗部とを上下方向に交互に配列して形成するようにしたことを特徴とする光照射型培養装置とする。   In order to solve the above-mentioned problems, the present invention corresponds to claim 1, a culture tank for storing a culture solution for suspending photosynthetic organisms, and a light-transmitting partition tube provided inside the culture tank A diffuser tube for blowing gas bubbles into the culture solution stored in the culture tank, and an illuminating means for illuminating the culture solution in the culture tank And the lighting means alternately arranges bright and dark portions in the vertical direction on the inner region and the outer peripheral region of the partition tube to collect and irradiate the illumination light from the light source. The light irradiation type culture apparatus is characterized in that it is formed.

又、上記構成において、照明手段を、仕切筒の内側の領域及び外周側の領域に照明光を照射する光源と、該光源の培養液に臨む側に配置した明部暗部交互形成手段とを備えてなるものとした構成とする。   Further, in the above configuration, the illumination means includes a light source for irradiating illumination light to the inner region and the outer peripheral region of the partition tube, and bright and dark portion alternate forming means arranged on the side of the light source facing the culture solution. It is set as the structure which becomes.

更に、上記構成において、明部暗部交互形成手段を、凸レンズ又はスリットを上下方向に配列して備えてなるものとした構成とする。   Further, in the above configuration, the bright and dark portion alternate forming means is configured to include convex lenses or slits arranged in the vertical direction.

更に又、上記構成において、明部暗部交互形成手段を、内筒部材と外筒部材との間に光源を収納する内部空間を備えた中空二重筒構造としてある仕切筒の上記内筒部材と外筒部材の壁面に、水平方向に延びる凸レンズ又はスリットを上下方向に複数配列して設けてなるものとした構成とする。   Furthermore, in the above-described configuration, the inner cylinder member of the partition cylinder having a hollow double cylinder structure having an inner space for storing a light source between the inner cylinder member and the outer cylinder member, and A plurality of convex lenses or slits extending in the horizontal direction are provided on the wall surface of the outer cylinder member in the vertical direction.

本発明の光照射型培養装置によれば、以下のような優れた効果を発揮する。
(1)光合成生物を懸濁する培養液を貯留する培養槽と、該培養槽の内側に設けた光透過性を有する仕切筒と、上記仕切筒の下端側開口部の下方に設けてガスの気泡を上記培養槽内に貯留した培養液に吹き込むための散気管と、上記培養槽内の培養液を照明するための照明手段を備え、且つ該照明手段は、上記仕切筒の内側の領域及び外周側の領域に、光源からの照明光を集光して照射する明部と、暗部とを上下方向に交互に配列して形成するようにした構成としてあるので、散気管より吹き込まれて仕切筒の内側を上昇する気泡によるエアリフト効果によって培養槽内で培養液を循環させるときに、該培養液に懸濁されている光合成生物を、予め形成してある明部と暗部を交互に通過させることができて、フラッシングライト効果による光合成の促進を図って、該光合成生物の増殖の促進化を図ることができる。
(2)又、上記明部は、光源からの照明光を集光して照射することで形成してあるため、上記培養液の濁度が高まっていても、該培養液の奥深くまで明部を形成させることができる。よって、光エネルギーの利用効率を高めることができる。
According to the light irradiation type culture apparatus of the present invention, the following excellent effects are exhibited.
(1) A culture tank for storing a culture solution in which photosynthetic organisms are suspended; a light-transmitting partition provided inside the culture tank; and a gas cylinder provided below a lower end opening of the partition. An aeration tube for blowing air bubbles into the culture solution stored in the culture tank; and illumination means for illuminating the culture solution in the culture tank, and the illumination means includes a region inside the partition tube and Since the bright part and the dark part that condense and irradiate the illumination light from the light source are arranged alternately in the vertical direction in the outer peripheral area, the partition is blown from the air diffuser. When circulating the culture solution in the culture tank by the air lift effect caused by the bubbles rising inside the tube, the photosynthetic organisms suspended in the culture solution are alternately passed through the light and dark parts that are formed in advance. Can be light by flashing light effect The aim of promoting the growth, can be promoted of the growth of the optical synthetic organisms.
(2) Moreover, since the bright part is formed by collecting and irradiating illumination light from a light source, even if the turbidity of the culture broth is increased, the bright part is deep into the culture broth. Can be formed. Therefore, the utilization efficiency of light energy can be improved.

本発明の光照射型培養装置の実施の一形態を示す概略切断側面図である。It is a general | schematic cutting side view which shows one Embodiment of the light irradiation type culture apparatus of this invention. 図1のA−A方向矢視図である。It is an AA direction arrow line view of FIG. 図1の装置の照明手段に備えた明部暗部交互形成手段の詳細な構成を示す断面図である。It is sectional drawing which shows the detailed structure of the bright part dark part alternating formation means with which the illumination means of the apparatus of FIG. 1 was equipped. 本発明の実施の他の形態として、照明手段の明部暗部交互形成手段の別の例を示す図3に対応する図である。It is a figure corresponding to FIG. 3 which shows another example of the bright part dark part alternating formation means of an illumination means as another form of implementation of this invention. 本発明の実施の更に他の形態として、照明手段の明部暗部交互形成手段の更に別の例を示す図3に対応する図である。It is a figure corresponding to FIG. 3 which shows another example of the bright part dark part alternating formation means of an illumination means as another form of implementation of this invention. 本発明の実施の更に他の形態を示す概略切断側面図である。It is a general | schematic cutting side view which shows other form of implementation of this invention.

以下、本発明を実施するための形態を図面を参照して説明する。   Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings.

図1乃至図3は本発明の光照射型培養装置の実施の一形態として、光合成生物としての微細藻類を培養対象とする場合の例を示すもので、以下のようにしてある。   FIG. 1 to FIG. 3 show an example of the case where microalgae as photosynthetic organisms are to be cultured as one embodiment of the light irradiation type culture apparatus of the present invention.

すなわち、培養対象である上記微細藻類を懸濁する培養液2を貯留するための培養槽1は、その中央部分に、上下方向に延びる筒形としたエアリフト用の仕切筒3を設ける。   That is, the culture tank 1 for storing the culture solution 2 for suspending the above-mentioned microalgae to be cultured is provided with a partition cylinder 3 for airlift that is formed in a cylindrical shape extending in the vertical direction at the center portion thereof.

上記培養槽1は、その底部における上記仕切筒3の下方に、散気管4を設ける。   The culture tank 1 is provided with a diffuser tube 4 below the partition tube 3 at the bottom thereof.

上記散気管4は、培養槽1の外部に設けた図示しないガス供給部より加圧されたガスを導くためのガス供給ライン5に接続する。なお、上記したように培養対象とする光合成生物が微細藻類の場合は、その光合成の際に二酸化炭素を必要とする。そこで、本実施の形態では、上記ガス供給ライン5を通して上記散気管4へ供給するガスは、培養液2中にて気泡を形成できるガスであって、且つ二酸化炭素を含むガスとして、たとえば、空気6を用いるようにしてある。   The aeration tube 4 is connected to a gas supply line 5 for introducing pressurized gas from a gas supply unit (not shown) provided outside the culture tank 1. When the photosynthetic organism to be cultured is a microalga as described above, carbon dioxide is required for the photosynthesis. Therefore, in the present embodiment, the gas supplied to the diffusion tube 4 through the gas supply line 5 is a gas that can form bubbles in the culture solution 2 and contains carbon dioxide, for example, air 6 is used.

これにより、上記散気管4は、図示しないガス供給部よりガス供給ライン5を通して導かれる加圧された空気6を、上記培養槽1内に貯留されている培養液2に対して上記散気管4を通して気泡として吹き込むことができるようにしてある。更に、この散気管4より上記培養液2中を浮上する気泡は、上記仕切筒3の内側を通って上昇するようにしてある。したがって、この仕切筒3の内側を上記空気6の気泡が上昇することに伴って、該仕切筒3の内側に存在する培養液2は、エアリフト効果により図1に矢印aで示すような上昇流(以下、上昇流aと云う)となるようにしてある。又、上記仕切筒3の内側での上昇流aに起因して、該仕切筒3の外側に存在する培養液2は、図1に矢印bで示す如き下降流(以下、下降流bと云う)となるようにしてある。よって、上記培養槽1は、貯留された培養液2全体に、上記仕切筒3の内側から順に、該仕切筒3の上側、外側、下側を通って再び該仕切筒3の内側に戻る循環流を形成させることができるように構成されている。   As a result, the air diffuser 4 allows the pressurized air 6 guided through the gas supply line 5 from a gas supply unit (not shown) to the culture medium 2 stored in the culture tank 1. It can be blown through as a bubble. Further, the air bubbles floating in the culture solution 2 from the air diffuser 4 rise through the inside of the partition tube 3. Therefore, as the bubbles of the air 6 rise inside the partition tube 3, the culture solution 2 existing inside the partition tube 3 flows upward as shown by an arrow a in FIG. (Hereinafter referred to as upward flow a). Further, due to the upward flow a inside the partition tube 3, the culture solution 2 existing outside the partition tube 3 flows downward as shown by the arrow b in FIG. 1 (hereinafter referred to as downward flow b). ). Therefore, the culture tank 1 circulates through the stored culture solution 2 through the upper side, the outer side, and the lower side of the partition tube 3 in order from the inner side of the partition tube 3 and returns to the inner side of the partition tube 3 again. It is comprised so that a flow can be formed.

更に、上記培養槽1は、上記培養液2を照明するための照明手段7を備える。該照明手段7は、上記培養槽1内における上記仕切筒3の内側に形成される培養液2の上昇流a、及び、該仕切筒3の外側に形成される培養液2の下降流bに対し、その流れ方向、すなわち、上下方向に、光源からの照明光を集光して照射する明部と、暗部を交互に形成することができるように構成されている。   Furthermore, the culture tank 1 is provided with illumination means 7 for illuminating the culture solution 2. The illuminating means 7 is applied to the upward flow a of the culture solution 2 formed inside the partition tube 3 in the culture tank 1 and the downward flow b of the culture solution 2 formed outside the partition tube 3. On the other hand, a bright part and a dark part that collect and irradiate the illumination light from the light source can be alternately formed in the flow direction, that is, in the vertical direction.

詳述すると、上記培養槽1は、たとえば、ステンレス製として上端側に開口を有し、下端を閉塞した円筒状の槽本体8と、該槽本体8の開口を塞ぐための蓋9とから構成されている。上記槽本体8は、上端側開口の周縁にフランジ部8aが設けてある。該フランジ部8aは、上記蓋9の外周部をボルト、ナットやバックル等の図示しない固定手段を介して取り外し可能に固定できるようにしてある。   More specifically, the culture tank 1 is made of, for example, stainless steel and has an opening on the upper end side, a cylindrical tank body 8 with the lower end closed, and a lid 9 for closing the opening of the tank body 8. Has been. The said tank main body 8 is provided with the flange part 8a in the periphery of the upper end side opening. The flange portion 8a is configured such that the outer peripheral portion of the lid 9 can be detachably fixed through fixing means (not shown) such as a bolt, a nut and a buckle.

更に、上記槽本体8は、少なくとも内周面(内側面)が鏡面加工してあり、これにより、後述するように上記照明手段7による照明光を有効利用できるようにしてある。   Furthermore, at least the inner peripheral surface (inner side surface) of the tank body 8 is mirror-finished so that the illumination light from the illumination means 7 can be used effectively as will be described later.

上記仕切筒3は、光透過性を有する素材製の透明な内筒部材10と、その外側に所定の隙間を隔てて同心状に配置した光透過性を有する素材製の透明な外筒部材11の各上端部同士と各下端部同士の間に、それぞれリング状の天井部材12と底部材13を水密に取り付けて、上記内筒部材10と外筒部材11との間に内部空間14を有する中空二重筒構造としてある。   The partition cylinder 3 includes a transparent inner cylinder member 10 made of a light-transmitting material and a transparent outer cylinder member 11 made of a light-transmitting material concentrically arranged on the outside with a predetermined gap. A ring-shaped ceiling member 12 and a bottom member 13 are attached in a watertight manner between the respective upper end portions and the respective lower end portions, and an internal space 14 is provided between the inner cylindrical member 10 and the outer cylindrical member 11. It has a hollow double cylinder structure.

更に、上記天井部材12は、周方向の複数個所に、上下に貫通する開口部15が設けてある。該周方向に複数個の開口部15は、上記照明手段7を構成するために後述するように該仕切筒3の内部空間14に周方向所定間隔で配列させて収納すべき複数の光源としての蛍光灯18を配置するときの周方向配置に対応するように設定してある。   Further, the ceiling member 12 is provided with openings 15 penetrating vertically at a plurality of locations in the circumferential direction. The plurality of openings 15 in the circumferential direction serve as a plurality of light sources to be arranged and stored in the inner space 14 of the partition tube 3 at predetermined intervals in the circumferential direction as will be described later in order to constitute the illumination means 7. It is set so as to correspond to the circumferential arrangement when the fluorescent lamp 18 is arranged.

上記仕切筒3の天井部材12の上記各開口部15の周縁部には、上下方向に所定寸法延びる支持部材としての個別の筒部材16の下端部が水密に取り付けてある。   At the peripheral edge of each opening 15 of the ceiling member 12 of the partition tube 3, a lower end portion of an individual tube member 16 as a support member extending a predetermined dimension in the vertical direction is attached in a watertight manner.

更に、上記各筒部材16は、上記培養槽1の蓋9を貫通させて上端側を上方へ突出させるようにする。そのために、上記蓋9には、各筒部材16にそれぞれ対応する配置で上下方向に貫通する開口部17が設けてあり、該各開口部17の下面側周縁部に、該開口部17に挿通させた上記各筒部材16が気密に取り付けてある。   Furthermore, each said cylindrical member 16 penetrates the lid | cover 9 of the said culture tank 1, and makes it project an upper end side upwards. For this purpose, the lid 9 is provided with an opening portion 17 penetrating in the vertical direction in an arrangement corresponding to each cylindrical member 16, and is inserted into the opening portion 17 at the lower peripheral edge portion of each opening portion 17. The above-described tubular members 16 are attached in an airtight manner.

これにより、上記蓋9には、上記仕切筒3が、上記各筒部材16を介して支持されるようにしてある。更に、該蓋9の各開口部17に取り付けてある各筒部材16は、その内側を通して、上記仕切筒3の内部空間14を外部に連通させることができるようにしてある。   Thus, the partition tube 3 is supported on the lid 9 via the tube members 16. Furthermore, each cylindrical member 16 attached to each opening 17 of the lid 9 can communicate the internal space 14 of the partition cylinder 3 to the outside through the inside thereof.

したがって、上記仕切筒3を支持させた状態の蓋9は、上記槽本体8の上端側開口に取り付けて培養槽1を形成することにより、上記仕切筒3を、上記培養槽1の中央部分に、槽本体8と同心状配置で、且つ該仕切筒3の下端と、上記培養槽1の内底面となる槽本体8の内底面との間に所定の隙間を隔てた状態に配置して固定できるようにしてある。なお、この際、上記培養槽1の中央部分に配置された上記仕切筒3の上端は、該培養槽1に所定量の培養液2を貯留するときの液面レベルよりも所定寸法下方に配置できるように、上記仕切筒3の上下寸法、及び、各筒部材16の上下寸法が設定してあるものとする。   Therefore, the lid 9 in a state where the partition tube 3 is supported is attached to the upper end side opening of the tank body 8 to form the culture tank 1, so that the partition cylinder 3 is placed at the central portion of the culture tank 1. In a concentric arrangement with the tank body 8 and fixed with a predetermined gap between the lower end of the partition tube 3 and the inner bottom surface of the tank body 8 serving as the inner bottom surface of the culture tank 1. I can do it. At this time, the upper end of the partition tube 3 disposed in the center portion of the culture tank 1 is disposed below the liquid level when a predetermined amount of the culture solution 2 is stored in the culture tank 1 by a predetermined dimension. It is assumed that the vertical dimension of the partitioning cylinder 3 and the vertical dimension of each cylindrical member 16 are set so that they can be performed.

これにより、上記培養槽1の内部に上記所定の液面レベルまで貯留された培養液2は、液面下に所定寸法没する該仕切筒3の上側に周方向に取り付けられている各筒部材16の間の隙間を通して、該仕切筒3の内外方向に自在に流通できるようにしてある。   As a result, each of the tubular members attached in the circumferential direction to the upper side of the partition tube 3 in which the culture liquid 2 stored up to the predetermined liquid level in the culture tank 1 is submerged by a predetermined dimension below the liquid level. Through the gap between 16, the partition tube 3 can freely flow in and out of the partition tube 3.

又、上記のように培養液2の液面下に没するように配置された仕切筒3は、内部空間14を備えた中空二重筒構造としてあるため、該仕切筒3に浮力が作用するようになる。そのため、この浮力は、上記各筒部材16を介して上記蓋9へ伝えて受けさせることができるようにしてある。   Further, since the partition tube 3 arranged so as to be immersed below the liquid surface of the culture solution 2 has a hollow double tube structure including the internal space 14, buoyancy acts on the partition tube 3. It becomes like this. Therefore, this buoyancy can be transmitted to and received by the lid 9 via each cylindrical member 16.

上記培養槽1の槽本体8の内底部の中央部分には、上記仕切筒3の下端開口部の平面形状以内となる領域に対応させて散気管4が設置してある。該散気管4のガス入口4aは、上記槽本体8の底板を内外方向に貫通させて外方へ突出させてあり、このガス入口4aに、上記ガス供給ライン5が接続してある。これにより、散気管4には、上記培養槽1内に貯留された培養液2によって該培養槽1の底部に作用している水圧よりも高い圧力まで加圧した空気6が、上記ガス供給ライン5を経て上記散気管4のガス入口4aより供給されることになる。上記散気管4より培養槽1内の培養液2に対して吹き込まれた空気6は、気泡として上記仕切筒3の内側を上昇させられるようにしてある。   In the central portion of the inner bottom portion of the tank body 8 of the culture tank 1, an air diffuser 4 is installed corresponding to a region that is within the planar shape of the lower end opening of the partition tube 3. The gas inlet 4a of the air diffuser pipe 4 penetrates the bottom plate of the tank body 8 inward and outward and protrudes outward. The gas supply line 5 is connected to the gas inlet 4a. Thereby, the air 6 pressurized to a pressure higher than the water pressure acting on the bottom of the culture tank 1 by the culture solution 2 stored in the culture tank 1 is supplied to the gas supply line 4 in the diffusion tube 4. Then, the gas is supplied from the gas inlet 4 a of the air diffuser 4 through 5. The air 6 blown into the culture solution 2 in the culture tank 1 from the air diffuser 4 is allowed to rise inside the partition tube 3 as bubbles.

上記散気管4より培養液2に吹き込む空気6の量は、調整することができ、該散気管4より上記仕切筒3の内側を上昇させる気泡の量を調整することにより、上記仕切筒3の内側に存在する培養液2に対して作用させるエアリフト効果の強度を変化させることができるようにしてある。これにより、上記培養槽1では、貯留された培養液2全体を、上記仕切筒3の内側から順に該仕切筒3の上側、外側、下側に通して再び該仕切筒3の内側に戻す循環流とさせるときの速さ(強度)を制御することができ、上記培養槽1内での培養液2の局所的な滞留や、培養対象である微細藻類の滞積を防止して、該培養槽1内の培養液2の懸濁状態を均一に保持することができるようにしてある。   The amount of air 6 blown into the culture solution 2 from the diffuser tube 4 can be adjusted, and by adjusting the amount of bubbles that lift the inside of the partition tube 3 from the diffuser tube 4, The strength of the air lift effect that acts on the culture medium 2 existing inside can be changed. Thereby, in the culture tank 1, the entire stored culture solution 2 is circulated back from the inside of the partition tube 3 to the inside of the partition tube 3 again through the upper side, the outside, and the lower side of the partition tube 3. The speed (strength) when flowing can be controlled, and the culture solution 2 in the culture tank 1 is prevented from staying locally and stagnant with the microalgae to be cultured. The suspension state of the culture solution 2 in the tank 1 can be maintained uniformly.

更に、上記培養液2に空気6を吹き込むときは、吹き込まれる空気6の気泡より、該培養液2に対して、微細藻類の光合成の際に消費される二酸化炭素を供給できるようにしてある。   Further, when the air 6 is blown into the culture solution 2, carbon dioxide consumed in the photosynthesis of microalgae can be supplied to the culture solution 2 from the bubbles of the blown air 6.

上記照明手段7は、図1に示すように、上記蓋9の開口部17に取り付けてある筒部材16に挿入可能な太さで上下方向に延びる蛍光灯ホルダ19を備えている。該蛍光灯ホルダ19は、その下端側に、蛍光灯18の一端を取り付けて、蛍光灯18が上下に延びる姿勢で保持できるようにしてある。該蛍光灯ホルダ19は該蛍光灯18に電力供給して点灯させることができるようにしてある。   As shown in FIG. 1, the illumination means 7 includes a fluorescent lamp holder 19 that extends in the vertical direction with a thickness that can be inserted into a cylindrical member 16 attached to the opening 17 of the lid 9. One end of the fluorescent lamp 18 is attached to the lower end side of the fluorescent lamp holder 19 so that the fluorescent lamp 18 can be held in a vertically extending posture. The fluorescent lamp holder 19 can be turned on by supplying power to the fluorescent lamp 18.

上記蛍光灯18を保持させた蛍光灯ホルダ19は、上記培養槽1の蓋9の各開口部17に取り付けてある各筒部材16に、該蓋9の上方より個別に差し込んで該各筒部材16の内側に装着するようにしてある。上記各蛍光灯ホルダ19に保持してある各蛍光灯18は、上記蓋9の各開口部17と、各筒部材16の内側、及び、上記仕切筒3における天井部材12の各開口部15を通して、該仕切筒3の内部空間14へ挿入して配置できるようにしてある。これにより、上記仕切筒3の内部空間14には、周方向に所定の間隔で、複数の蛍光灯18が光源として配置されることになる。   The fluorescent lamp holder 19 holding the fluorescent lamp 18 is individually inserted from above the lid 9 into each cylindrical member 16 attached to each opening 17 of the lid 9 of the culture tank 1. 16 is mounted inside. Each fluorescent lamp 18 held in each fluorescent lamp holder 19 passes through each opening 17 of the lid 9, the inside of each cylindrical member 16, and each opening 15 of the ceiling member 12 in the partition cylinder 3. , And can be arranged by being inserted into the internal space 14 of the partition tube 3. As a result, a plurality of fluorescent lamps 18 are arranged as light sources in the inner space 14 of the partition tube 3 at predetermined intervals in the circumferential direction.

したがって、上記仕切筒3の内部空間14に周方向に複数配置された各蛍光灯18は、点灯させることにより、光が発せられる。該各蛍光灯18より発せられる光は、仕切筒3の透明な内筒部材10を透過させて、該内筒部材10の内側となる仕切筒3の内側(軸心側)に存在する培養液2に対して照射できるようにしてある。同時に、上記各蛍光灯18より発せられる光は、透明な外筒部材11を透過させて、該外筒部材11の外周側となる仕切筒3の外周側に存在する培養液2に対しても照射できるようにしてある。   Therefore, a plurality of fluorescent lamps 18 arranged in the circumferential direction in the inner space 14 of the partition tube 3 are lit to emit light. The light emitted from each fluorescent lamp 18 is transmitted through the transparent inner cylinder member 10 of the partition tube 3, and is present inside the partition tube 3 (on the axial center side) that is inside the inner tube member 10. 2 can be irradiated. At the same time, the light emitted from each of the fluorescent lamps 18 is transmitted through the transparent outer cylinder member 11 and also to the culture solution 2 existing on the outer peripheral side of the partition tube 3 that is the outer peripheral side of the outer cylindrical member 11. It can be irradiated.

又、本発明の光照射型培養装置は、微細藻類の培養中において、上記仕切筒3の内部空間14に複数配置された上記各蛍光灯18のうち、いずれかの蛍光灯18が寿命に達して点滅が生じたり、点灯しなくなった場合は、該寿命に達した蛍光灯18の蛍光灯ホルダ19を、上記筒部材16より培養槽1の蓋9の上方へ抜き出すようにして取り外すことができるようにしてある。したがって、本発明の光照射型培養装置は、培養槽1内での培養を一時中断したり、培養槽1の蓋9を取り外す作業を必要とすることなく、培養を継続しながら上記寿命に達した蛍光灯18の交換作業を実施することができるようにしてある。   Further, in the light irradiation type culture apparatus of the present invention, during the cultivation of microalgae, any one of the fluorescent lamps 18 arranged in the internal space 14 of the partition tube 3 reaches the end of its life. When the flashing occurs or the lighting stops, the fluorescent lamp holder 19 of the fluorescent lamp 18 that has reached the end of its life can be removed by pulling it out above the lid 9 of the culture tank 1 from the tubular member 16. It is like that. Therefore, the light irradiation type culture apparatus of the present invention reaches the above-mentioned lifetime while continuing the culture without requiring the operation of temporarily suspending the culture in the culture tank 1 or removing the lid 9 of the culture tank 1. The fluorescent lamp 18 can be replaced.

更に、本実施の形態における照明手段7は、上記培養槽1内で仕切筒3の内側と外側に形成される培養液2の上昇流aと下降流bに対して、その流れ方向である上下方向に、照明光を集光して照射する明部と、暗部を交互に形成するために、明部暗部交互形成手段を備える。   Furthermore, the illumination means 7 in the present embodiment is an upper and lower direction which is the flow direction with respect to the upward flow a and the downward flow b of the culture solution 2 formed inside and outside the partition tube 3 in the culture tank 1. In order to alternately form a bright part and a dark part for condensing and irradiating illumination light in the direction, a bright part dark part alternating forming unit is provided.

上記明部暗部交互形成手段は、上記仕切筒3の透明な内筒部材10と透明な外筒部材11に設けるようにしてある。すなわち、上記内筒部材10は、内周面に、水平方向となる周方向に連続して軸心側へ突出する凸部10aと、周方向に連続して軸心より離反する側へ凹む凹部10bとを、上下方向に後述する所定の間隔で交互に設けて、該内筒部材10の内周面の断面が上下方向に波型形状となるようにした構成としてある。更に、上記外筒部材11は、外周面に、水平方向となる周方向に連続して軸心より離反する側へ突出する凸部11aと、周方向に連続して軸心に近接する側へ凹む凹部11bとを、上下方向に後述する所定の間隔で交互に設けて、該外筒部材11の外周面の断面が上下方向に波型形状となるようにした構成としてある。   The bright part dark part alternate forming means is provided on the transparent inner cylinder member 10 and the transparent outer cylinder member 11 of the partition cylinder 3. That is, the inner cylinder member 10 has, on the inner peripheral surface thereof, a convex portion 10a that protrudes toward the axial center continuously in the circumferential direction that is the horizontal direction, and a concave portion that is recessed toward the side away from the axial center continuously in the circumferential direction. 10b are alternately provided in the vertical direction at predetermined intervals, which will be described later, so that the cross section of the inner peripheral surface of the inner cylinder member 10 has a wave shape in the vertical direction. Further, the outer cylinder member 11 has, on the outer peripheral surface thereof, a convex portion 11a that protrudes toward the side away from the axis continuously in the circumferential direction that is the horizontal direction, and a side that is adjacent to the axis continuously in the circumferential direction. Recessed recesses 11b are alternately provided in the vertical direction at predetermined intervals, which will be described later, so that the outer peripheral surface of the outer cylinder member 11 has a corrugated shape in the vertical direction.

なお、上記構成としてある明部暗部交互形成手段は、上記内筒部材10の凸部10aと凹部10bが設けてある内周面、及び、上記外筒部材11の凸部11aと凹部11bが設けてある外周面が、共に培養液2に接する面になる。この点に鑑みて、上記内筒部材10を構成する光透過性を有する透明な素材と、上記外筒部材11を構成する光透過性を有する透明な素材は、共に上記培養液2との間で屈折率に差がある素材としてある。   In addition, the light part dark part alternating formation means which is the said structure is provided with the internal peripheral surface in which the convex part 10a and the recessed part 10b of the said inner cylinder member 10 are provided, and the convex part 11a and the recessed part 11b of the said outer cylinder member 11. Both outer peripheral surfaces are in contact with the culture medium 2. In view of this point, the transparent material having optical transparency constituting the inner cylindrical member 10 and the transparent material having optical transparency constituting the outer cylindrical member 11 are both between the culture solution 2 and The material has a difference in refractive index.

これにより、上記内筒部材10は、図3に示すように、内周面に上記各凸部10aが設けてある部分の周壁が、周方向に連続する平凸レンズになる。このため、上記仕切筒3の内部空間14に設置してある各蛍光灯18より発せられた光は、上記各凸部10aが設けてある部分の内筒部材10の周壁を透過すると、該内筒部材10の素材と上記培養液2との屈折率の差に基づく屈折により、該各凸部10aの頂点を含む水平面に存在する焦点f1へ向けて上下両側より集光されるようになる。   Thereby, as shown in FIG. 3, the said inner cylinder member 10 becomes a plano-convex lens in which the peripheral wall of the part in which each said convex part 10a is provided in the internal peripheral surface continues in the circumferential direction. For this reason, when the light emitted from each fluorescent lamp 18 installed in the internal space 14 of the partition tube 3 passes through the peripheral wall of the inner cylinder member 10 in the portion where the projections 10a are provided, Due to refraction based on the difference in refractive index between the material of the tubular member 10 and the culture solution 2, light is condensed from both the upper and lower sides toward the focal point f1 existing on the horizontal plane including the apex of each convex portion 10a.

一方、上記内筒部材10の内周面に上記各凹部10bが設けてある部分の周壁は、周方向に連続する平凹レンズになる。このため、上記仕切筒3の内部空間14に設置してある各蛍光灯18より発せられた光は、上記各凹部10bが設けてある部分の周壁を透過すると、上下方向への拡散が行われるようになる。   On the other hand, the peripheral wall of the portion where the concave portions 10b are provided on the inner peripheral surface of the inner cylinder member 10 is a plano-concave lens that is continuous in the circumferential direction. For this reason, when the light emitted from the fluorescent lamps 18 installed in the internal space 14 of the partition tube 3 passes through the peripheral wall of the portion where the concave portions 10b are provided, the light is diffused in the vertical direction. It becomes like this.

よって、上記仕切筒3の内側の領域には、仕切筒3の内筒部材10の内周面に上記各凸部10aが設けてある位置に対応する上下方向所定間隔個所毎に、上記各蛍光灯18より発せられる光が集光された状態で照射される明部が形成され、且つ上記内筒部材10の内周面に上記各凹部10bが設けてある位置に対応する上下方向所定間隔個所毎に、上記各明部に比して光量が低下した暗部が形成されるようになる。したがって、該仕切筒3の内側の領域には、上下方向に上記明部と暗部が交互に形成されるようになる。   Therefore, in the inner region of the partition tube 3, each of the fluorescent lights is arranged at predetermined intervals in the vertical direction corresponding to the positions where the protrusions 10 a are provided on the inner peripheral surface of the inner tube member 10 of the partition tube 3. Bright portions irradiated with the light emitted from the lamp 18 being condensed are formed, and predetermined intervals in the vertical direction corresponding to positions where the concave portions 10b are provided on the inner peripheral surface of the inner cylinder member 10. Each time, a dark portion is formed in which the amount of light is lower than that of each of the bright portions. Therefore, in the region inside the partition tube 3, the bright part and the dark part are alternately formed in the vertical direction.

なお、図3に示すように、上記内筒部材10の内周面に上記各凸部10aが設けてある部分の周壁が平凸レンズとして機能するときの焦点f1は、図3に一点鎖線で示す如き該内筒部材10の軸心位置Oに配置されるように設定することが望ましい。このようにすれば、上記内筒部材10の内周面に上記各凸部10aが設けてある部分の周壁の周方向の一個所で集光された光は、上記焦点f1を通過した後に拡散するときには、同じ凸部10aが設けてある部分の周壁の周方向の180度反対側の個所で集光されて上記焦点f1へ向かう光の経路とほぼ同じ経路を反対向きに通るようになる。このため、仕切筒3の内側の領域は、上記内筒部材10の各凸部10aが設けてある上下方向位置(高さ位置)毎に形成する明部に光をより効率よく集めることができて、該明部と暗部との光量の差をより大きくすることができる。   In addition, as shown in FIG. 3, the focus f1 when the peripheral wall of the part in which each said convex part 10a is provided in the inner peripheral surface of the said inner cylinder member 10 functions as a plano-convex lens is shown with a dashed-dotted line in FIG. It is desirable to set so as to be arranged at the axial center position O of the inner cylinder member 10 as described above. In this way, the light collected at one place in the circumferential direction of the peripheral wall of the portion where the convex portions 10a are provided on the inner peripheral surface of the inner cylindrical member 10 diffuses after passing through the focal point f1. In this case, the light is condensed at a position 180 degrees opposite to the circumferential direction of the peripheral wall of the portion where the same convex portion 10a is provided, and passes through almost the same path as the light path toward the focal point f1 in the opposite direction. For this reason, the area | region inside the partition cylinder 3 can gather light more efficiently in the bright part formed for every up-down direction position (height position) in which each convex part 10a of the said inner cylinder member 10 is provided. Thus, the difference in light quantity between the bright part and the dark part can be further increased.

又、上記外筒部材11には、上記内筒部材10と同様に、図3に示すように、該外筒部材11の外周面に上記各凸部11aが設けてある部分の周壁が、周方向に連続する平凸レンズになる。このため、上記仕切筒3の内部空間14に設置してある各蛍光灯18より発せられた光は、上記各凸部11aが設けてある部分の周壁を透過すると、該外筒部材11の素材と上記培養液2との屈折率の差に基づく屈折により、該各凸部11aの頂点を含む水平面に存在する焦点f2へ向けて上下両側より集光されるようになる。   Further, as shown in FIG. 3, the outer cylinder member 11 is provided with a peripheral wall of a portion where the convex portions 11 a are provided on the outer peripheral surface of the outer cylinder member 11, as in the case of the inner cylinder member 10. The plano-convex lens is continuous in the direction. For this reason, when the light emitted from each fluorescent lamp 18 installed in the internal space 14 of the partition tube 3 passes through the peripheral wall of the portion where the projections 11a are provided, the material of the outer tube member 11 By the refraction based on the difference in refractive index between the liquid and the culture solution 2, the light is condensed from both the upper and lower sides toward the focal point f2 existing on the horizontal plane including the apex of each convex portion 11a.

一方、上記外筒部材11の外周面に上記各凹部11bが設けてある部分の周壁は、周方向に連続する平凹レンズになる。このため、上記仕切筒3の内部空間14に設置してある各蛍光灯18より発せられた光は、上記各凹部11bが設けてある部分の周壁を透過すると、上下方向への拡散が行われるようになる。   On the other hand, the peripheral wall of the portion where the concave portions 11b are provided on the outer peripheral surface of the outer cylinder member 11 is a plano-concave lens that is continuous in the circumferential direction. For this reason, when the light emitted from each fluorescent lamp 18 installed in the internal space 14 of the partition tube 3 passes through the peripheral wall of the portion where the concave portions 11b are provided, the light is diffused in the vertical direction. It becomes like this.

よって、上記仕切筒3の外周側の領域には、仕切筒3の外筒部材11の外周面に上記各凸部11aが設けてある位置に対応する上下方向所定間隔個所毎に、上記各蛍光灯18より発せられる光が集光された状態で照射される明部が形成され、且つ上記外筒部材11の外周面に上記各凹部11bが設けてある位置に対応する上下方向所定間隔個所毎に、上記明部に比して光量が低下した暗部が形成されるようになる。したがって、該仕切筒3の外周側の領域には、上下方向に上記明部と暗部が交互に形成されるようになる。   Therefore, in the region on the outer peripheral side of the partition tube 3, the fluorescence is provided at predetermined intervals in the vertical direction corresponding to the positions where the convex portions 11 a are provided on the outer peripheral surface of the outer tube member 11 of the partition tube 3. Bright portions irradiated with the light emitted from the lamp 18 being condensed are formed, and every predetermined interval in the vertical direction corresponding to the position where each of the concave portions 11b is provided on the outer peripheral surface of the outer cylinder member 11. In addition, a dark portion having a light amount lower than that of the bright portion is formed. Therefore, in the region on the outer peripheral side of the partition tube 3, the bright part and the dark part are alternately formed in the vertical direction.

なお、図3に示すように、上記外筒部材11の外周面に上記各凸部11aが設けてある部分の周壁が平凸レンズとして機能するときの焦点f2は、該外筒部材11を取り囲むように配置されている培養槽1の槽本体8の内周面(内側面)の位置に配置されるように設定することが望ましい。このようにすれば、上記外筒部材11の外周面に上記各凸部11aが設けてある部分の周壁で集光された光は、上記焦点f2を通過すると同時に、前述したように鏡面加工してある上記槽本体8の内周面で反射されるようになるため、該焦点f2を通過した後に拡散するときには、外筒部材11の外周面に上記各凸部11aが設けてある部分の周壁で集光されてそれぞれ対応する焦点f2へ向かう光の経路とほぼ同じ経路を反対向きに通るようになる。このため、仕切筒3の外周側の領域は、上記外筒部材11の各凸部11aが設けてある上下方向位置(高さ位置)毎に形成する明部に光をより効率よく集めることができて、該明部と暗部との光量の差をより大きくすることができる。   As shown in FIG. 3, the focal point f <b> 2 when the peripheral wall of the portion where the convex portions 11 a are provided on the outer peripheral surface of the outer cylindrical member 11 functions as a plano-convex lens surrounds the outer cylindrical member 11. It is desirable to set so that it may be arrange | positioned in the position of the internal peripheral surface (inner side surface) of the tank main body 8 of the culture tank 1 arrange | positioned. In this way, the light collected on the peripheral wall of the outer peripheral surface of the outer cylindrical member 11 where the convex portions 11a are provided passes through the focal point f2 and is mirror-finished as described above. Therefore, when the light diffuses after passing through the focal point f2, the peripheral wall of the portion where the convex portions 11a are provided on the outer peripheral surface of the outer cylinder member 11 The light passes through approximately the same path as the light path toward the corresponding focal point f2 in the opposite direction. For this reason, the area | region of the outer peripheral side of the partition cylinder 3 can gather light more efficiently in the bright part formed for every up-down direction position (height position) in which each convex part 11a of the said outer cylinder member 11 is provided. Thus, the difference in light quantity between the bright part and the dark part can be further increased.

上記仕切筒3の内筒部材10の内周面に設ける凸部10aと凹部10bの上下方向の配列間隔、及び、外筒部材11の外周面に設ける凸部11aと凹部11bの上下方向の配列間隔は以下のように設定すればよい。   The vertical arrangement interval of the convex portions 10a and the concave portions 10b provided on the inner peripheral surface of the inner cylinder member 10 of the partition tube 3, and the vertical arrangement of the convex portions 11a and the concave portions 11b provided on the outer peripheral surface of the outer cylindrical member 11 The interval may be set as follows.

すなわち、前述したように培養槽1内に貯留されている培養液2は、散気管4より吹き込む空気6の気泡によるエアリフト効果によって該培養槽1内に貯留した培養液2全体に循環流が形成させられる。このときに、培養槽1内での培養液2の局所的な滞留や、培養対象である微細藻類の滞積を防止するという観点、及び、散気管4より培養槽1内の培養液2に対して吹き込む気泡の量は培養液2に過剰なバブリングが生じないように設定するという観点から考えると、上記培養槽1内の培養液2に形成させる上記循環流の流速は、或る程度の範囲に定まる。よって、この循環流に伴って仕切筒3の内側の領域に生じる培養液2の上昇流aの流速もある程度定まるようになる。   That is, as described above, the culture solution 2 stored in the culture vessel 1 forms a circulating flow in the entire culture solution 2 stored in the culture vessel 1 due to the air lift effect caused by the bubbles of the air 6 blown from the diffusion tube 4. Be made. At this time, from the viewpoint of preventing the local accumulation of the culture solution 2 in the culture tank 1 and the accumulation of microalgae to be cultured, and the culture solution 2 in the culture tank 1 from the aeration tube 4 From the viewpoint of setting the amount of air bubbles to be blown so that excessive bubbling does not occur in the culture solution 2, the flow rate of the circulating flow formed in the culture solution 2 in the culture tank 1 is a certain level. Determined by the range. Therefore, the flow velocity of the upward flow a of the culture solution 2 generated in the inner region of the partition tube 3 with this circulation flow is also determined to some extent.

そこで、上記仕切筒3の内筒部材10の内周面に設ける各凸部10a及び各凹部10bの上下方向の配列間隔は、上記仕切筒3の内側の領域に生じる培養液2の上昇流aに伴われて該上昇流aの流速で移動する微細藻類が、上記内筒部材10の内周面に設けた各凸部10aと各凹部10bによって該領域に形成される明部と暗部を交互に通るときの時間間隔が、予め実験等により得られる上記微細藻類の光合成の促進に最適なフラッシングライト効果(明反応、暗反応サイクル効果)を与える光の明滅サイクルに一致するように、適宜設定するようにすればよい。   Therefore, the vertical arrangement interval between the convex portions 10a and the concave portions 10b provided on the inner peripheral surface of the inner cylinder member 10 of the partition tube 3 is the upward flow a of the culture solution 2 generated in the inner region of the partition tube 3. Accordingly, the microalgae that move at the flow velocity of the upward flow a alternate between the bright and dark portions formed in the region by the convex portions 10a and the concave portions 10b provided on the inner peripheral surface of the inner cylindrical member 10. The time interval when passing through is set as appropriate so as to match the light flashing cycle that gives the optimal flashing light effect (bright reaction, dark reaction cycle effect) for promoting the photosynthesis of the microalgae obtained in advance through experiments, etc. You just have to do it.

又、上記培養槽1内の培養液2に形成させる上記循環流の流速は、上記のように或る程度の範囲に定まるようになることから、該循環流に伴って仕切筒3の外周側の領域に生じる培養液2の下降流bの流速もある程度定まるようになる。   Further, since the flow rate of the circulating flow formed in the culture solution 2 in the culture tank 1 is determined within a certain range as described above, the outer peripheral side of the partition tube 3 is associated with the circulating flow. The flow rate of the descending flow b of the culture solution 2 generated in the region is determined to some extent.

そこで、上記仕切筒3の外筒部材11の外周面に設ける各凸部11a及び各凹部11bの上下方向の配列間隔は、上記仕切筒3の外周側の領域に生じる培養液2の下降流bに伴われて該下降流bの流速で移動する微細藻類が、上記外筒部材11の外周面に設けた各凸部11aと各凹部11bによって該領域に形成される明部と暗部を交互に通るときの時間間隔が、上記微細藻類の光合成の促進に最適なフラッシングライト効果を与える光の明滅サイクルに一致するように、適宜設定するようにすればよい。   Therefore, the vertical spacing between the convex portions 11a and the concave portions 11b provided on the outer peripheral surface of the outer cylinder member 11 of the partition tube 3 is the downward flow b of the culture solution 2 generated in the outer peripheral side region of the partition tube 3. Accordingly, the microalgae that move at the flow velocity of the downward flow b alternate between the bright and dark portions formed in the region by the convex portions 11a and the concave portions 11b provided on the outer peripheral surface of the outer cylinder member 11. What is necessary is just to make it set suitably so that the time interval when passing may correspond to the light flickering cycle which gives the flushing light effect optimal for promotion of the photosynthesis of the said micro algae.

なお、図示してないが、上記培養槽1は、培養対象である光合成生物としての微細藻類の種培養と培養液2を供給するための共通あるいは個別の供給ライン、微細藻類が懸濁された培養液を取り出すための培養液排出ライン、排気ライン、培養途中で微細藻類が懸濁された培養液2をサンプルとして取り出すためのサンプル採取手段、培養液2を目視により観察できるようにするための観察窓、培養液2の濁度を測定する手段や、培養液2の温度を調整するための温度調整手段、更には、上記微細藻類の培養プロセスで必要とされる薬品等を培養液2へ投入するための投入手段等、上記培養槽1内における微細藻類の培養の過程で必要とされる作業を実施するための機器が装備してあるものとする。   In addition, although not shown in figure, the said culture tank 1 is a common or separate supply line for supplying the seed culture of the microalgae as a photosynthetic organism to be cultured and the culture solution 2, and microalgae are suspended. A culture solution discharge line for taking out the culture solution, an exhaust line, a sample collecting means for taking out the culture solution 2 in which microalgae are suspended in the middle of the culture as a sample, and enabling the culture solution 2 to be observed visually An observation window, a means for measuring the turbidity of the culture solution 2, a temperature adjustment means for adjusting the temperature of the culture solution 2, and further, chemicals and the like necessary for the above-mentioned microalgae cultivation process are supplied to the culture solution 2. It is assumed that equipment for performing operations required in the process of culturing microalgae in the culture tank 1, such as input means for input, is provided.

以上の構成としてある本発明の光照射型培養装置は、これを使用する場合、培養槽1には、培養対象となる上記微細藻類の種培養と、培養液2を供給して、該培養槽1内に、該微細藻類を懸濁してなる培養液2を貯留させ、この培養液2中に仕切筒3を没水させるようにする。   When the light irradiation type culture apparatus of the present invention having the above-described configuration is used, the culture tank 1 is supplied with the seed culture of the above-mentioned microalgae to be cultured and the culture solution 2, and the culture tank A culture solution 2 in which the microalgae are suspended is stored in 1, and the partition tube 3 is submerged in the culture solution 2.

その後、上記仕切筒3の内部空間14に配置してある各蛍光灯18は、光源として点灯させると共に、上記仕切筒3には、散気管4に加圧されて導かれた空気6が培養槽1内の培養液2中に気泡として吹き込まれるようにする。培養液2中に吹き込まれた空気6は、気泡として上記仕切筒3の内側を上昇するときにエアリフト効果を生じさせる。該エアリフト効果により、上記培養槽1内の培養液2は、上記仕切筒3の内側では上昇流aとなり、該仕切筒3の上側では隣接する筒部材16同士の間を通して該仕切筒3の内側から外側へ移動し、該仕切筒3の外周側では下降流bとなり、その後、該仕切筒3の下側を通って該仕切筒3の内側へ再び戻るという図1に矢印で示す如き流路を循環流として循環させられる。   Thereafter, each fluorescent lamp 18 disposed in the internal space 14 of the partition tube 3 is turned on as a light source, and the partition tube 3 is supplied with air 6 pressurized and guided by the air diffuser 4. The air is blown into the culture medium 2 in 1 as bubbles. The air 6 blown into the culture medium 2 causes an air lift effect when rising inside the partition tube 3 as bubbles. Due to the air lift effect, the culture medium 2 in the culture tank 1 becomes an upward flow a inside the partition tube 3 and passes between adjacent tube members 16 on the inside of the partition tube 3 to the inside of the partition tube 3. The flow path as shown by the arrow in FIG. 1 moves from the outside to the outside, becomes a downward flow b on the outer peripheral side of the partition tube 3, and then returns to the inside of the partition tube 3 through the lower side of the partition tube 3. Is circulated as a circulating flow.

この際、上記仕切筒3の内側の領域には、該仕切筒3の内筒部材10の内周面に上下方向に交互に設けてある凸部10aと凹部10bにより、上記各蛍光灯18の光を集光して照射した明部と、暗部が、上下方向に交互に形成されているため、上記仕切筒3の内側の領域で上昇流aとなる培養液2に含まれている上記微細藻類は、該領域の明部と暗部とを交互に通過することでフラッシングライト効果(明反応、暗反応サイクル効果)を受けるようになる。   At this time, in the inner region of the partition tube 3, the projections 10 a and the recesses 10 b provided alternately in the vertical direction on the inner peripheral surface of the inner tube member 10 of the partition tube 3, Since the bright part and dark part which condensed and irradiated light are formed in the up-and-down direction alternately, the said fine contained in the culture solution 2 used as the upward flow a in the area | region inside the said partition cylinder 3 Algae receives a flashing light effect (bright reaction, dark reaction cycle effect) by alternately passing through the light and dark parts of the region.

又、上記仕切筒3の外周側の領域には、該仕切筒3の外筒部材11の外周面に上下方向に交互に設けてある凸部11aと凹部11bにより、上記各蛍光灯18の光を集光して照射した明部と、暗部が、上下方向に交互に形成されているため、上記仕切筒3の外周側の領域で下降流bとなる培養液2に含まれている上記微細藻類は、該領域の明部と暗部とを交互に通過することでフラッシングライト効果(明反応、暗反応サイクル効果)を受けるようになる。   Moreover, in the area | region of the outer peripheral side of the said partition cylinder 3, the light of each said fluorescent lamp 18 is carried out by the convex part 11a and the recessed part 11b which were alternately provided in the up-down direction by the outer peripheral surface of the outer cylinder member 11 of this partition cylinder 3. Since the bright part and the dark part which are condensed and irradiated are alternately formed in the vertical direction, the fine portion contained in the culture solution 2 which becomes the downward flow b in the outer peripheral side region of the partition tube 3. Algae receives a flashing light effect (bright reaction, dark reaction cycle effect) by alternately passing through the light and dark parts of the region.

上記仕切筒3は、内側の領域、及び、外周側の領域に上下方向に交互に配置された明部と暗部は予め形成してあるため、該各領域で上昇流aや下降流bとなる培養液2の濁度にかかわらず、該上昇流aや下降流bとなっている培養液2中に含まれている微細藻類は、上記フラッシングライト効果を受けて増殖が促進されるようになる。   In the partition tube 3, the bright and dark portions alternately arranged in the vertical direction in the inner region and the outer peripheral region are formed in advance, so that the upward flow a and the downward flow b are generated in each region. Regardless of the turbidity of the culture solution 2, the microalgae contained in the culture solution 2 in the upward flow a and the downward flow b receive the above-mentioned flushing light effect and are promoted to grow. .

よって、培養液2に懸濁されている光合成生物としての微細藻類は、その量が少なくて、該培養液2の濁度が低い培養初期であっても、上記フラッシングライト効果により光合成が促進される。このため、上記微細藻類の増殖を促すことができる。   Therefore, the microalgae as the photosynthetic organisms suspended in the culture solution 2 have a small amount and the photosynthesis is promoted by the flushing light effect even in the early stage of the culture when the turbidity of the culture solution 2 is low. The For this reason, the growth of the microalgae can be promoted.

又、上記仕切筒3の内側及び外周側に形成してある各明部は、光源である蛍光灯18の光を集光して照射することで形成してある。このため、上記各明部では、上記培養液2に懸濁されている上記微細藻類の量が増加して該培養液2の濁度が高まっていても、上記集光された光を照射することで、該濁度の高い培養液2の奥深くまで明部を形成させることができる。よって、光エネルギーの利用効率を高めることができる。   The bright portions formed on the inner and outer peripheral sides of the partition tube 3 are formed by collecting and irradiating the light from the fluorescent lamp 18 that is a light source. For this reason, in each said bright part, even if the quantity of the said micro algae suspended in the said culture solution 2 increases and the turbidity of this culture solution 2 is increasing, the said condensed light is irradiated. Thereby, a bright part can be formed in the deep inside of the culture solution 2 with high turbidity. Therefore, the utilization efficiency of light energy can be improved.

このように、本発明の光照射型培養装置によれば、微細藻類の培養液2が、培養初期の濁度が低い状態、及び、該培養液の濁度が高まった状態のいずれにおいても、該培養液中の微細藻類に対してフラッシングライト効果による増殖の促進を図ることができるため、該微細藻類の増殖効率を高めることができる。   As described above, according to the light irradiation type culture apparatus of the present invention, the microalgae culture solution 2 has a low turbidity at the initial stage of culture and a state in which the turbidity of the culture solution is increased. Since the growth of the microalgae in the culture solution can be promoted by the flushing light effect, the efficiency of the microalgae can be increased.

次に、図4は本発明の実施の他の形態として、図1乃至図3の実施の形態と同様の構成における照明手段7の明部暗部交互形成手段の別の例を示すものである。   Next, FIG. 4 shows another example of the bright part / dark part alternating forming unit of the illuminating unit 7 having the same configuration as the embodiment of FIGS. 1 to 3 as another embodiment of the present invention.

すなわち、図4に示す照明手段7は、図1乃至図3に示したものと同様に、中空二重筒構造とした仕切筒3の内部空間14に、周方向所定間隔で複数の蛍光灯18を収納してなる構成において、明部暗部交互形成手段として、上記仕切筒3の内筒部材10の内周面と、外筒部材11の外周面に、それぞれ凸部10a,11aと凹部10b,11bを上下方向に所定間隔で交互に配列して設けた構成としてあることに代えて、上記内筒部材10は、上記仕切筒3の内部空間14に臨む外周面に、周方向に連続して軸心より離反する側へ突出する凸部10cと、周方向に連続して軸心に近接する側へ凹む凹部10dとを、上下方向に所定の間隔で設けて、該内筒部材10の外周面の断面が上下方向に波型形状となるようにしてある。   That is, the illuminating means 7 shown in FIG. 4 has a plurality of fluorescent lamps 18 at predetermined intervals in the circumferential direction in the inner space 14 of the partition tube 3 having a hollow double tube structure, as in the case shown in FIGS. Are formed on the inner peripheral surface of the inner cylinder member 10 and the outer peripheral surface of the outer cylinder member 11 as the light and dark portion alternating forming means, respectively, as convex portions 10a, 11a and concave portions 10b, In place of the configuration in which 11b is alternately arranged at predetermined intervals in the vertical direction, the inner cylinder member 10 is continuously provided in the circumferential direction on the outer peripheral surface facing the inner space 14 of the partition cylinder 3. An outer periphery of the inner cylinder member 10 is provided with a convex portion 10c protruding to the side away from the shaft center and a concave portion 10d recessed continuously to the side close to the shaft center in the vertical direction at predetermined intervals. The cross section of the surface is wavy in the vertical direction.

更に、上記外筒部材11は、上記仕切筒3の内部空間14に臨む内周面に、周方向に連続して軸心側へ突出する凸部11cと、周方向に連続して軸心より離反する側へ凹む凹部11dとを、上下方向に所定の間隔で設けて、該外筒部材11の内周面の断面が上下方向に波型形状となるようにした構成としてある。   Further, the outer cylinder member 11 is formed on the inner peripheral surface facing the inner space 14 of the partition cylinder 3 by a convex portion 11c continuously protruding in the circumferential direction toward the axial center side and continuously from the axial center in the circumferential direction. Concave portions 11d that are recessed toward the separating side are provided at predetermined intervals in the vertical direction so that the cross section of the inner peripheral surface of the outer cylinder member 11 has a wave shape in the vertical direction.

なお、上記内筒部材10の内周面と、外筒部材11の外周面は、それぞれ凹凸のない単純な円筒面としてある。   The inner peripheral surface of the inner cylindrical member 10 and the outer peripheral surface of the outer cylindrical member 11 are simple cylindrical surfaces having no irregularities.

その他の構成は図1乃至図3に示したものと同様であり、同一のものには同一の符号が付してある。   Other configurations are the same as those shown in FIGS. 1 to 3, and the same components are denoted by the same reference numerals.

以上の構成としてある本実施の形態の照明手段7における明部暗部交互形成手段によれば、上記仕切筒の内筒部材10の上下方向における外周面に各凸部10cが設けてある部分の周壁は、周方向に延びる平凸レンズとして機能して、蛍光灯18の光を該各凸部10cの頂点を含む平面内に存在する焦点f1に向けて集光させることができる。   According to the bright part dark part alternate forming means in the illumination means 7 of the present embodiment having the above-described configuration, the peripheral wall of the portion where each convex part 10c is provided on the outer peripheral surface in the vertical direction of the inner cylinder member 10 of the partition cylinder Functions as a plano-convex lens extending in the circumferential direction, and can condense the light from the fluorescent lamp 18 toward a focal point f1 existing in a plane including the apex of each convex portion 10c.

一方、上記仕切筒の内筒部材10の上下方向における外周面に各凹部10dが設けてある部分の周壁は、周方向に延びる平凹レンズとして機能して、蛍光灯18の光を上下方向に拡散させることができる。   On the other hand, the peripheral wall of the portion where the concave portions 10d are provided on the outer peripheral surface in the vertical direction of the inner cylinder member 10 of the partition tube functions as a plano-concave lens extending in the circumferential direction, and diffuses the light from the fluorescent lamp 18 in the vertical direction. Can be made.

又、上記仕切筒の外筒部材11においても、内周面に各凸部11cが設けてある部分の周壁は、周方向に延びる平凸レンズとして機能して、蛍光灯18の光を該各凸部11cの頂点を含む平面内に存在する焦点f2に向けて集光させることができる。   Also in the outer cylinder member 11 of the partition tube, the peripheral wall of the portion where the convex portions 11c are provided on the inner peripheral surface functions as a plano-convex lens extending in the circumferential direction, and the light from the fluorescent lamp 18 is transmitted to the convex portions. The light can be condensed toward the focal point f2 existing in the plane including the vertex of the portion 11c.

一方、上記仕切筒の外筒部材11の上下方向における内周面に各凹部11dが設けてある部分の周壁は、周方向に延びる平凹レンズとして機能して、蛍光灯18の光を上下方向に拡散させることができる。   On the other hand, the peripheral wall of the portion where each concave portion 11d is provided on the inner peripheral surface in the vertical direction of the outer cylinder member 11 of the partition tube functions as a plano-concave lens extending in the circumferential direction, and allows the light from the fluorescent lamp 18 to move in the vertical direction. Can be diffused.

したがって、上記蛍光灯18の光を上記内筒部材10を透過させることにより、上記仕切筒3の内側の領域には、上記内筒部材10における凸部10cと凹部10dの上下方向の配置に対応して、上記蛍光灯18の光が集光されて照射される明部と、暗部とが上下方向に交互に形成されるようになる。   Therefore, by transmitting the light from the fluorescent lamp 18 through the inner tube member 10, the inner region of the partition tube 3 corresponds to the vertical arrangement of the protrusions 10c and the recesses 10d in the inner tube member 10. Thus, the bright portions and the dark portions irradiated with the light from the fluorescent lamp 18 condensed are formed alternately in the vertical direction.

又、上記蛍光灯18の光を上記外筒部材11を透過させることにより、上記仕切筒3の外周側の領域には、上記外筒部材11における凸部11cと凹部11dの上下方向の配置に対応して、上記蛍光灯18の光が集光されて照射される明部と、暗部とが上下方向に交互に形成されるようになる。   Further, by allowing the light from the fluorescent lamp 18 to pass through the outer cylinder member 11, the convex portion 11 c and the concave portion 11 d of the outer cylinder member 11 are arranged in the vertical direction in the region on the outer peripheral side of the partition tube 3. Correspondingly, bright portions and dark portions, which are collected and irradiated with the light from the fluorescent lamp 18, are alternately formed in the vertical direction.

したがって、上記図1乃至図3の実施の形態と同様に、上記培養槽1内の培養液2は、上記仕切筒3の内側では上昇流aとなり、該仕切筒3の上側では隣接する筒部材16同士の間を通して該仕切筒3の内側から外側へ移動し、該仕切筒3の外周側では下降流bとなり、その後、該仕切筒3の下側を通って該仕切筒3の内側へ再び戻るという流路を循環流として循環させられる。この間に、上記仕切筒3の内側の領域、及び、外周側の領域では、それぞれ上昇流a、及び、下降流bとなる培養液2に含まれている微細藻類に対して、上記各領域の明部と暗部とを交互に通過することによるフラッシングライト効果(明反応、暗反応サイクル効果)を与えることができる。   Accordingly, as in the embodiment of FIGS. 1 to 3, the culture solution 2 in the culture tank 1 becomes an upward flow a inside the partition tube 3, and an adjacent cylinder member above the partition tube 3. It moves from the inside of the partition cylinder 3 to the outside through the space between 16 and becomes a downward flow b on the outer peripheral side of the partition cylinder 3, and then returns to the inside of the partition cylinder 3 through the lower side of the partition cylinder 3. The return flow path is circulated as a circulation flow. In the meantime, in the inner region of the partition tube 3 and the outer peripheral region, the microalgae contained in the culture solution 2 that becomes the upward flow a and the downward flow b, respectively, A flashing light effect (bright reaction, dark reaction cycle effect) can be provided by alternately passing the light part and the dark part.

よって、本実施の形態によっても、上記図1乃至図3に示す実施の形態と同様の効果を得ることができる。   Therefore, according to this embodiment, the same effect as that of the embodiment shown in FIGS. 1 to 3 can be obtained.

更に、上記仕切筒3は、内筒部材10における培養液2と接する内周面、及び、外筒部材11における培養液2に接する外周面に、凹凸がない。このため、上記仕切筒3では、内筒部材10の内周面に接する培養液2の流れ、及び、外筒部材11の外周面に接する側での培養液2の流れが乱されなくなるため、上記培養槽1内における培養液2の循環をより円滑に行わせることができて、該循環流を形成させるために散気管4(図1参照)より培養液2に気泡として吹き込むことが必要とされる空気6の使用量を低減させることができる。   Further, the partition tube 3 has no irregularities on the inner peripheral surface of the inner tube member 10 that contacts the culture solution 2 and the outer peripheral surface of the outer tube member 11 that contacts the culture solution 2. For this reason, in the partition tube 3, the flow of the culture solution 2 in contact with the inner peripheral surface of the inner cylinder member 10 and the flow of the culture solution 2 on the side in contact with the outer peripheral surface of the outer cylinder member 11 are not disturbed. The culture medium 2 can be circulated more smoothly in the culture tank 1, and in order to form the circulation flow, it is necessary to blow into the culture medium 2 from the air diffuser 4 (see FIG. 1) as bubbles. The amount of air 6 used can be reduced.

次いで、図5は本発明の実施の更に他の形態として、図1乃至図3の実施の形態と同様の構成における照明手段7の明部暗部交互形成手段の別の例を示すものである。   Next, FIG. 5 shows another example of the bright part / dark part alternating forming unit of the illuminating unit 7 having the same configuration as the embodiment of FIGS. 1 to 3 as still another embodiment of the present invention.

すなわち、本実施の形態における照明手段7は、図1乃至図3に示したものと同様に、中空二重筒構造とした仕切筒3の内部空間14に、周方向所定間隔で複数の蛍光灯18を収納してなる構成において、明部暗部交互形成手段として、上記仕切筒3の内筒部材10の内周面と、外筒部材11の外周面に、それぞれ凸部10a,11aと凹部10b,11bを上下方向に所定間隔で交互に配列して設けた構成としてあることに代えて、上記仕切筒3の内筒部材10と外筒部材11は、凹凸のない単純な円筒形状とする。更に、後述する所定幅の帯状とし且つ少なくとも一方の面を鏡面のような反射面21としてなる反射部材20a及び20bが、上記内筒部材10及び外筒部材11の上下方向所定間隔の複数個所に、上記反射面21を仕切筒3の内部空間14の内側に臨ませた姿勢で水平方向となる周方向の全周に亘り取り付けてある。この際、上記内筒部材10に上下方向に配列して取り付けられた各反射部材20aと、上記外筒部材11に上下方向に配列して取り付けられた各反射部材20bは、上下方向に互い違いの配置となるようにする。   That is, the illuminating means 7 in the present embodiment has a plurality of fluorescent lamps at predetermined intervals in the circumferential direction in the inner space 14 of the partition tube 3 having a hollow double tube structure, similar to the one shown in FIGS. In the configuration in which 18 is housed, as the bright and dark portion alternate forming means, the convex portions 10a and 11a and the concave portion 10b are formed on the inner peripheral surface of the inner cylinder member 10 and the outer peripheral surface of the outer cylinder member 11, respectively. , 11b are alternately arranged at predetermined intervals in the vertical direction, and the inner cylinder member 10 and the outer cylinder member 11 of the partition cylinder 3 have a simple cylindrical shape with no irregularities. Further, reflecting members 20a and 20b having a band shape with a predetermined width, which will be described later, and having at least one surface as a reflecting surface 21 such as a mirror surface, are provided at a plurality of predetermined intervals in the vertical direction of the inner cylinder member 10 and the outer cylinder member 11. The reflection surface 21 is attached to the entire circumference in the circumferential direction, which is the horizontal direction, with the posture facing the inside of the internal space 14 of the partition tube 3. At this time, the reflecting members 20a arranged in the vertical direction on the inner cylinder member 10 and the reflecting members 20b arranged in the vertical direction on the outer cylinder member 11 are alternately arranged in the vertical direction. To be arranged.

なお、上記各反射部材20a,20bは、培養槽1内での培養液2の循環流の流れに影響が生じないようにするという観点、及び、上記各反射部材20a,20bの反射面21の汚れを防止するという観点から考えると、図5に示すように、内筒部材10では、仕切筒3の内部空間14に臨む外周面に取り付け、又、外筒部材11では上記仕切筒3の内部空間14に臨む内周面に取り付けるようにすることが望ましい。   In addition, each said reflecting member 20a, 20b does not produce influence on the flow of the circulating flow of the culture solution 2 in the culture tank 1, and the reflecting surface 21 of each said reflecting member 20a, 20b. From the viewpoint of preventing dirt, as shown in FIG. 5, the inner cylinder member 10 is attached to the outer peripheral surface facing the inner space 14 of the partition cylinder 3, and the outer cylinder member 11 is inside the partition cylinder 3. It is desirable to attach to the inner peripheral surface facing the space 14.

なお、上記反射部材20aと20bは、一方、又は、双方について、上記反射部材20aを上記内筒部材10の内周面に取り付ける構成や、上記反射部材20bを上記外筒部材11の外周面に取り付ける構成を採用することが可能であることは勿論である。   In addition, the reflection member 20a and 20b are the structure which attaches the said reflection member 20a to the internal peripheral surface of the said inner cylinder member 10, or the said reflection member 20b in the outer peripheral surface of the said outer cylinder member 11 about one or both. Of course, it is possible to adopt a mounting configuration.

これにより、上記内筒部材10には、上下方向に配列して取り付けられた各反射部材20aの上下方向に隣接するもの同士の間に、それぞれ光を通過させることが可能なスリット22aを形成する。上記内筒部材10の各スリット22aは、上記仕切筒3の内部空間14に設けてある各蛍光灯18より発せられた後に該各スリット22aへ直接向かう直接光と、上記各蛍光灯18より発せられた光が上記外筒部材11に取り付けてある各反射部材20bの反射面21で反射された後に上記内筒部材10のスリット22aへ向かう反射光が集光されて、該スリット22aを透過して上記仕切筒3の内側の領域へ照射させられるようにしてある。   As a result, the inner cylinder member 10 is formed with slits 22a through which light can pass between adjacent ones of the reflecting members 20a arranged in the vertical direction and adjacent to each other in the vertical direction. . The slits 22a of the inner cylinder member 10 are emitted from the fluorescent lamps 18 provided in the internal space 14 of the partition cylinder 3 and then emitted directly from the fluorescent lamps 18 to the slits 22a. After the reflected light is reflected by the reflecting surface 21 of each reflecting member 20b attached to the outer cylinder member 11, the reflected light toward the slit 22a of the inner cylinder member 10 is condensed and transmitted through the slit 22a. Thus, the region inside the partition tube 3 is irradiated.

したがって、該仕切筒3の内側の領域には、上記内筒部材10の各スリット22aが設けてある高さ位置毎に、上記蛍光灯18の光が集光されて照射される明部が形成され、該各明部同士の間に、上記内筒部材10に取り付けてある反射部材20aにより上記蛍光灯18の光が遮られる暗部が形成されるようになる。   Therefore, a bright portion where the light from the fluorescent lamp 18 is condensed and irradiated is formed in the inner region of the partition tube 3 at every height position where each slit 22a of the inner tube member 10 is provided. Then, a dark portion where the light of the fluorescent lamp 18 is blocked by the reflecting member 20a attached to the inner cylinder member 10 is formed between the bright portions.

又、上記外筒部材11には、上下方向に配列して取り付けられた各反射部材20bの上下方向に隣接するもの同士の間に、それぞれ光を通過させることが可能なスリット22bを形成する。上記外筒部材11の各スリット22bは、上記仕切筒3の内部空間14に設けてある各蛍光灯18より発せられた後に該各スリット22bへ直接向かう直接光と、上記各蛍光灯18より発せられた光が上記内筒部材10に取り付けてある各反射部材20aの反射面21で反射された後に上記外筒部材11のスリット22bへ向かう反射光が集光されて、該スリット22bを透過して上記仕切筒3の外周側の領域へ照射させられるようにしてある。   The outer cylinder member 11 is formed with slits 22b through which light can pass between the reflecting members 20b arranged in the vertical direction and adjacent to each other in the vertical direction. The slits 22b of the outer cylinder member 11 are emitted from the fluorescent lamps 18 provided in the internal space 14 of the partition tube 3 and then emitted directly from the fluorescent lamps 18 to the slits 22b. After the reflected light is reflected by the reflecting surface 21 of each reflecting member 20a attached to the inner cylinder member 10, the reflected light toward the slit 22b of the outer cylinder member 11 is condensed and transmitted through the slit 22b. Thus, the region on the outer peripheral side of the partition tube 3 is irradiated.

したがって、該仕切筒3の外周側の領域には、上下方向における上記外筒部材11の各スリット22bが設けてある高さ位置毎に、上記蛍光灯18の光が集光されて照射される明部が形成され、該各明部同士の間に、上記外筒部材11に取り付けてある反射部材20bにより上記蛍光灯18の光が遮られる暗部が形成されるようになる。   Therefore, the light from the fluorescent lamp 18 is condensed and irradiated on the outer peripheral side region of the partition tube 3 at each height position where the slits 22b of the outer tube member 11 are provided in the vertical direction. A bright part is formed, and a dark part where the light from the fluorescent lamp 18 is blocked by the reflecting member 20b attached to the outer cylinder member 11 is formed between the bright parts.

ところで、上記内筒部材10の各スリット22aの上下方向の配列間隔と、上記外筒部材10の各スリット22bの上下方向の配列間隔は、上記内筒部材10と外筒部材11に取り付ける各反射部材20a,20bの幅寸法に応じて定まる。   By the way, the vertical arrangement interval of the slits 22 a of the inner cylinder member 10 and the vertical arrangement interval of the slits 22 b of the outer cylinder member 10 are the reflections attached to the inner cylinder member 10 and the outer cylinder member 11. It is determined according to the width dimension of the members 20a and 20b.

そこで、上記各反射部材20a,20bの幅寸法は、上記内筒部材10のスリット22a及び上記外筒部材11のスリット22bの上下方向の配列間隔を、図1乃至図3の実施の形態にて仕切筒3の内筒部材10と外筒部材11にそれぞれ設けた各凸部10a,11aの上下方向の配列間隔と同様の配列間隔とさせることができるように設定すればよい。   Therefore, the width dimension of each of the reflecting members 20a and 20b is determined by the vertical arrangement interval of the slits 22a of the inner cylinder member 10 and the slits 22b of the outer cylinder member 11 in the embodiment shown in FIGS. What is necessary is just to set so that it can be set as the arrangement | positioning space | interval similar to the arrangement space | interval of the up-down direction of each convex part 10a, 11a provided in the inner cylinder member 10 and the outer cylinder member 11 of the partition cylinder 3, respectively.

なお、本実施の形態では、上記内筒部材10に取り付ける反射部材20aと、上記外筒部材11に取り付ける反射部材20bとを、上下方向に互い違いの配置にすることから、上記培養槽1については、仕切筒3の内側の領域での培養液2の流路断面積と、該仕切筒3の外周側の領域での培養液2の流路断面積がほぼ同様となるように設計して、上記仕切筒3の内側の領域に生じる培養液2の上昇流aの流速と、該仕切筒3の外周側の領域に生じる培養液2の下降流bの流速がほぼ同様となるようにしてある。   In addition, in this Embodiment, since the reflection member 20a attached to the said inner cylinder member 10 and the reflection member 20b attached to the said outer cylinder member 11 are arrange | positioned alternately at an up-down direction, about the said culture tank 1, The flow path cross-sectional area of the culture solution 2 in the inner region of the partition tube 3 and the flow channel cross-sectional area of the culture solution 2 in the outer peripheral side region of the partition tube 3 are designed to be substantially the same. The flow rate of the upward flow a of the culture solution 2 generated in the inner region of the partition tube 3 is substantially the same as the flow rate of the downward flow b of the culture solution 2 generated in the outer peripheral region of the partition tube 3. .

又、図示する便宜上、図5では、上記蛍光灯18より発せられた光が各反射部材20a,20bの反射面21で反射されるときの経路を屈曲させて記載してある。   For convenience of illustration, FIG. 5 shows a bent path when the light emitted from the fluorescent lamp 18 is reflected by the reflecting surface 21 of each reflecting member 20a, 20b.

その他の構成は図1乃至図3に示したものと同様であり、同一のものには同一の符号が付してある。   Other configurations are the same as those shown in FIGS. 1 to 3, and the same components are denoted by the same reference numerals.

以上の構成としてある本実施の形態の照明手段7における明部暗部交互形成手段によっても、上記仕切筒3の内側の領域には、上記蛍光灯18の光が集光されて照射される明部と、暗部とを上下方向に交互に形成することができ、又、上記仕切筒3の外周側の領域には、上記蛍光灯18の光が集光されて照射される明部と、暗部とを上下方向に交互に形成することができる。   Also by the bright part dark part alternate forming means in the illumination means 7 of the present embodiment having the above-described configuration, the light part where the light from the fluorescent lamp 18 is condensed and irradiated on the inner region of the partition tube 3. And dark portions can be alternately formed in the vertical direction, and in the region on the outer peripheral side of the partition tube 3, a bright portion and a dark portion where the light from the fluorescent lamp 18 is condensed and irradiated Can be alternately formed in the vertical direction.

したがって、上記図1乃至図3の実施の形態と同様に、上記培養槽1内の培養液2は、循環させると、上記仕切筒3の内側の領域、及び、外周側の領域では、それぞれ上昇流a、及び、下降流bとなる培養液2に含まれている微細藻類に対して、上記各領域の明部と暗部とを交互に通過することによるフラッシングライト効果(明反応、暗反応サイクル効果)を与えることができる。   Therefore, as in the embodiment of FIGS. 1 to 3, when the culture medium 2 in the culture tank 1 is circulated, it rises in the inner region of the partition tube 3 and the outer peripheral region, respectively. Flushing light effect (bright reaction, dark reaction cycle) by passing the bright part and the dark part of each region alternately for the microalgae contained in the culture solution 2 that becomes the flow a and the downward flow b Effect).

よって、本実施の形態によっても、図1乃至図3の実施の形態と同様の効果を得ることができる。   Therefore, the present embodiment can provide the same effects as those of the embodiment of FIGS. 1 to 3.

図6は本発明の更に他の形態として、培養槽の外部に光源を設ける場合の例を示すものである。   FIG. 6 shows an example in which a light source is provided outside the culture tank as still another embodiment of the present invention.

すなわち、本実施の形態の光照射型培養装置は、培養槽1aの槽本体23として、光透過性を有する素材により製作された槽本体23を用いる。   That is, the light irradiation type culture apparatus of the present embodiment uses a tank body 23 made of a light-transmitting material as the tank body 23 of the culture tank 1a.

上記培養槽1aの中央部には、光透過性を有する素材製で且つ上下方向に延びる単純な円筒形状としてある仕切筒24を配置する。該仕切筒24は、上端部における周方向の複数個所に取り付けた支柱部材25の上端部を上記培養槽1aの蓋9の下面側に取り付けて支持するようにしてある。   A partition cylinder 24 made of a light-transmitting material and having a simple cylindrical shape extending in the vertical direction is disposed at the center of the culture tank 1a. The partition tube 24 is configured to attach and support the upper end portions of the column members 25 attached to a plurality of locations in the circumferential direction at the upper end portion on the lower surface side of the lid 9 of the culture tank 1a.

又、本実施の形態の光照射型培養装置は、照明手段7aの光源として、上記培養槽1aの槽本体23の外周位置に、周方向に所定間隔で複数の蛍光灯18を、上下方向に延びる姿勢で配置する。なお、上記各蛍光灯18は、該各蛍光灯18への電力供給を行うための図示しないホルダに保持させてあるものとする。   Moreover, the light irradiation type culture apparatus of this embodiment uses a plurality of fluorescent lamps 18 in the vertical direction at predetermined intervals in the circumferential direction as the light source of the illumination means 7a at the outer peripheral position of the tank body 23 of the culture tank 1a. Place in an extended posture. Each fluorescent lamp 18 is assumed to be held by a holder (not shown) for supplying power to each fluorescent lamp 18.

更に、本実施の形態の光照射型培養装置は、上記照明手段7aに備える明部暗部交互形成手段として、上記光透過性を有する槽本体23の側壁部の外周面に、水平方向となる周方向に連続して軸心より離反する側へ突出する凸部23aと、周方向に連続して軸心に近接する側へ凹む凹部23bとを、上下方向に交互に設けて、該槽本体23の外周面の断面が上下方向に波型形状となるようにしてある。   Furthermore, the light irradiation type culture apparatus of the present embodiment is a peripheral portion in the horizontal direction on the outer peripheral surface of the side wall portion of the light-transmitting tank body 23 as the light / dark portion alternating forming means provided in the illumination means 7a. Convex portions 23a projecting toward the side away from the axial center continuously in the direction and concave portions 23b recessed toward the side adjacent to the axial center continuously in the circumferential direction are alternately provided in the vertical direction, and the tank body 23 The cross-section of the outer peripheral surface of this is a wave shape in the vertical direction.

これにより、上記槽本体23の上下方向における外周面に上記各凸部23aが設けてある部分の周壁は、周方向に連続する平凸レンズになる。このため、上記蛍光灯18より発せられた光は、該各凸部23aが設けてある部分の周壁を透過すると、該各凸部23aの頂点を含む水平面に存在する焦点へ向けて上下両側より集光されるようになる。   Thereby, the peripheral wall of the part in which each said convex part 23a is provided in the outer peripheral surface in the up-down direction of the said tank main body 23 becomes a plano-convex lens continuous in the circumferential direction. For this reason, when the light emitted from the fluorescent lamp 18 passes through the peripheral wall of the portion where each convex portion 23a is provided, the light is emitted from both the upper and lower sides toward the focal point existing on the horizontal plane including the apex of each convex portion 23a. It will be condensed.

一方、上記槽本体23の上下方向における外周面に上記各凹部23bが設けてある部分の周壁は、周方向に連続する平凹レンズになる。このため、上記蛍光灯18より発せられた光が、該各凹部10bが設けてある部分の周壁を透過するときには、上下方向への拡散が行われるようになる。   On the other hand, the peripheral wall of the portion where the concave portions 23b are provided on the outer peripheral surface in the vertical direction of the tank body 23 is a plano-concave lens that is continuous in the circumferential direction. For this reason, when the light emitted from the fluorescent lamp 18 passes through the peripheral wall of the portion where the concave portions 10b are provided, the light is diffused in the vertical direction.

なお、本実施の形態では、上記槽本体23の外周面の上記各凸部23aが設けてある部分の周壁を透過するときに集光される蛍光灯18の光は、上記光透過性を有する仕切筒24の周壁を更に透過した後、該各凸部23aの頂点を含む水平面における該仕切筒24の軸心位置に焦点を結ぶように設定してある。   In the present embodiment, the light of the fluorescent lamp 18 that is condensed when passing through the peripheral wall of the outer peripheral surface of the tank body 23 where the convex portions 23a are provided has the light transmittance. After further passing through the peripheral wall of the partition tube 24, the focal point is set so as to focus on the axial center position of the partition tube 24 in the horizontal plane including the apex of each convex portion 23 a.

これにより、本実施の形態の光照射型培養装置は、上記照明手段7aの各蛍光灯18を点灯すると、上記培養槽1a内における仕切筒24の内側の領域と外周側の領域に対して、槽本体23の外周面に各凸部23aが設けてある高さ位置毎に、上記蛍光灯18の光が集光された明部を形成でき、又、槽本体23の外周面に各凹部23bが設けてある高さ位置毎に暗部を形成することができるようになる。よって、上記培養槽1a内における仕切筒24の内側の領域と外周側の領域には、上下方向に明部と暗部が交互に形成されるようになる。   Thereby, when the light irradiation type culture apparatus of this Embodiment lights each fluorescent lamp 18 of the said illumination means 7a, with respect to the area | region inside the partition cylinder 24 in the said culture tank 1a, and the area | region of an outer peripheral side, For each height position where each convex portion 23 a is provided on the outer peripheral surface of the tank body 23, a bright portion where the light from the fluorescent lamp 18 is condensed can be formed, and each concave portion 23 b is formed on the outer peripheral surface of the tank main body 23. It becomes possible to form a dark part at every height position provided with. Therefore, bright and dark portions are alternately formed in the vertical direction in the inner region and the outer peripheral region of the partition tube 24 in the culture tank 1a.

なお、本実施の形態では、上記槽本体23の外周面に設ける凸部23aの上下方向の配列間隔は、図1乃至図3の実施の形態にて仕切筒3の内筒部材10と外筒部材11にそれぞれ設けた各凸部10a,11aの上下方向の配列間隔と同様の配列間隔とさせることができるように設定すればよい。   In the present embodiment, the vertical arrangement interval of the convex portions 23a provided on the outer peripheral surface of the tank body 23 is the same as the inner cylinder member 10 and the outer cylinder of the partition cylinder 3 in the embodiment of FIGS. What is necessary is just to set so that it can be set as the arrangement | positioning space | interval similar to the arrangement | positioning space | interval of the up-down direction of each convex part 10a provided in the member 11 respectively.

なお、本実施の形態の場合には、図5の実施の形態の場合と同様に、上記仕切筒24の内側の領域に生じる培養液2の上昇流aの流速と、該仕切筒24の外周側の領域に生じる培養液2の下降流bの流速がほぼ同様となるようにしてあるものとする。   In the case of the present embodiment, as in the case of the embodiment of FIG. 5, the flow rate of the upward flow a of the culture solution 2 generated in the inner region of the partition tube 24 and the outer periphery of the partition tube 24 It is assumed that the flow rate of the descending flow b of the culture solution 2 generated in the side region is substantially the same.

その他の構成は図1乃至図3に示したものと同様であり、同一のものには同一の符号が付してある。   Other configurations are the same as those shown in FIGS. 1 to 3, and the same components are denoted by the same reference numerals.

以上の構成としてある本実施の形態の光照射型培養装置によれば、上記培養槽1a内で循環させる培養液2が、仕切筒24の内側の領域と外周側の領域をそれぞれ上昇流aと下降流bとして流れるときに、上記明部と暗部を交互に通過する該培養液2中の微細藻類に対し、フラッシングライト効果(明反応、暗反応サイクル効果)を与えることができる。   According to the light irradiation type culture device of the present embodiment having the above-described configuration, the culture solution 2 to be circulated in the culture tank 1a has an upward flow a and a region on the inner side and the outer peripheral side of the partition tube 24, respectively. When flowing as a downward flow b, a flushing light effect (bright reaction, dark reaction cycle effect) can be given to the microalgae in the culture solution 2 that alternately pass through the bright part and the dark part.

よって、本実施の形態によっても、図1乃至図3の実施の形態と同様の効果を得ることができる。   Therefore, the present embodiment can provide the same effects as those of the embodiment of FIGS. 1 to 3.

なお、本発明は上記実施の形態のみに限定されるものではなく、培養槽1,1aの径寸法と高さ、及び、該培養槽1,1a内に設ける仕切筒3,24の径寸法と高さは、いずれも図示するための便宜的な寸法や形状であって実際の寸法や形状を反映したものではない。   In addition, this invention is not limited only to the said embodiment, The diameter size and height of the culture tanks 1 and 1a, and the diameter dimension of the partition cylinders 3 and 24 provided in this culture tank 1 and 1a The heights are convenient dimensions and shapes for illustration, and do not reflect actual dimensions or shapes.

図1乃至図3の実施の形態、及び、図4の実施の形態における仕切筒3の内筒部材10と外筒部材11に設ける凸部10a,11aと凹部10b,11b、並びに、図6の実施の形態における培養槽1aの槽本体23に設ける凸部23aと凹部23bの曲率と、上下方向の配列間隔と、配列数は、いずれも図示するための便宜的なものであって実際の装置の構成を反映したものではない。   1 to 3 and the projections 10a and 11a and the recesses 10b and 11b provided on the inner cylinder member 10 and the outer cylinder member 11 of the partition cylinder 3 in the embodiment of FIG. In the embodiment, the curvatures of the protrusions 23a and the recesses 23b provided in the tank body 23 of the culture tank 1a, the arrangement interval in the vertical direction, and the number of arrangements are all for convenience of illustration, and are actual devices. It does not reflect the composition of

又、図1乃至図3の実施の形態、及び、図4の実施の形態の応用例として、上記仕切筒3の内筒部材10と外筒部材11の双方の内周面に凸部10a,11cと凹部10b,11dを上下方向に交互に設けるようにしたり、内筒部材10と外筒部材11の双方の外周面に凸部10c,11aと凹部10d,11bを上下方向に交互に設けるようにした構成を採用してもよい。   Moreover, as an application example of the embodiment of FIGS. 1 to 3 and the embodiment of FIG. 4, the convex portions 10 a, 10 a are formed on the inner peripheral surfaces of both the inner cylinder member 10 and the outer cylinder member 11 of the partition cylinder 3. 11c and recesses 10b and 11d are alternately provided in the vertical direction, or convex parts 10c and 11a and recesses 10d and 11b are alternately provided in the vertical direction on the outer peripheral surfaces of both the inner cylinder member 10 and the outer cylinder member 11. You may employ | adopt the structure made into.

更には、上記仕切筒3の内筒部材10及び外筒部材11は、内周面と外周面の両面に、上下方向に交互に配列される凸部と凹部を同位相で設けて、該内筒部材10と外筒部材11の周壁に、両凸レンズとして機能する部分と、両凹レンズとして機能する部分を、上下方向に交互に形成させてなる構成としてもよい。   Furthermore, the inner cylinder member 10 and the outer cylinder member 11 of the partition cylinder 3 are provided with convex portions and concave portions alternately arranged in the vertical direction on the both surfaces of the inner peripheral surface and the outer peripheral surface in the same phase. It is good also as a structure formed by alternately forming the part which functions as a biconvex lens and the part which functions as a biconcave lens in the surrounding wall of the cylinder member 10 and the outer cylinder member 11 in the up-down direction.

図6の実施の形態では、培養槽1aの槽本体23の内周面、あるいは、内周面と外周面の双方に、凸部と凹部10を上下方向に交互に設けた構成としてもよい。   In the embodiment of FIG. 6, a configuration may be adopted in which convex portions and concave portions 10 are alternately provided in the vertical direction on the inner peripheral surface of the tank body 23 of the culture tank 1 a or on both the inner peripheral surface and the outer peripheral surface.

照明手段7,7aにて周方向に配置する蛍光灯18の本数は、培養槽1,1a内で培養対象とする光合成生物の培養に必要とされる光の強度、及び、培養槽1,1aのサイズに応じて適宜変更してよい。   The number of fluorescent lamps 18 arranged in the circumferential direction by the illumination means 7 and 7a depends on the intensity of light required for culturing photosynthetic organisms to be cultured in the culture tanks 1 and 1a and the culture tanks 1 and 1a. You may change suitably according to the size.

本発明の光照射型培養装置は、培養槽1に貯留する培養液2に懸濁させた状態で培養することができる光合成生物であれば、微細藻類以外のいかなる光合成生物を培養する場合に適用してもよい。   The light irradiation type culture apparatus of the present invention is applicable to culturing any photosynthetic organism other than microalgae as long as it is a photosynthetic organism that can be cultured in a state suspended in the culture solution 2 stored in the culture tank 1. May be.

又、培養対象とする光合成生物に対応させて、培養液2に散気管4より気泡を吹き込むためガスとして、空気以外のガスを使用するようにしてもよい。   Further, gas other than air may be used as the gas in order to blow bubbles into the culture solution 2 from the diffuser tube 4 in correspondence with the photosynthetic organism to be cultured.

その他本発明の要旨を逸脱しない範囲内で種々変更を加え得ることは勿論である。   Of course, various modifications can be made without departing from the scope of the present invention.

1 培養槽
2 培養液
3 仕切筒
4 散気管
6 空気(ガス)
7,7a 照明手段
18 蛍光灯(光源)
22a,22b スリット
24 仕切筒
DESCRIPTION OF SYMBOLS 1 Culture tank 2 Culture solution 3 Partition cylinder 4 Aeration pipe 6 Air (gas)
7, 7a Illumination means 18 Fluorescent lamp (light source)
22a, 22b Slit 24 Partition tube

Claims (4)

光合成生物を懸濁する培養液を貯留する培養槽と、該培養槽の内側に設けた光透過性を有する仕切筒と、上記仕切筒の下端側開口部の下方に設けてガスの気泡を上記培養槽内に貯留した培養液に吹き込むための散気管と、上記培養槽内の培養液を照明するための照明手段を備え、且つ該照明手段は、上記仕切筒の内側の領域及び外周側の領域に、光源からの照明光を集光して照射する明部と、暗部とを上下方向に交互に配列して形成するようにしたことを特徴とする光照射型培養装置。   A culture tank for storing a culture solution in which photosynthetic organisms are suspended; a light-transmitting partition provided inside the culture tank; and a gas bubble provided below a lower end opening of the partition cylinder. A diffuser tube for blowing into the culture solution stored in the culture tank, and illumination means for illuminating the culture solution in the culture tank, and the illumination means are provided on the inner region and the outer peripheral side of the partition tube A light irradiation type culture apparatus characterized in that a bright part and a dark part for condensing and irradiating illumination light from a light source are alternately arranged in a vertical direction in a region. 照明手段を、仕切筒の内側の領域及び外周側の領域に照明光を照射する光源と、該光源の培養液に臨む側に配置した明部暗部交互形成手段とを備えてなるものとした請求項1記載の光照射型培養装置。   The illuminating means comprises a light source that irradiates illumination light to the inner region and the outer peripheral region of the partition tube, and bright / dark portion alternating forming means disposed on the side of the light source facing the culture solution. Item 4. The light irradiation type culture apparatus according to Item 1. 明部暗部交互形成手段を、凸レンズ又はスリットを上下方向に配列して備えてなるものとした請求項2記載の光照射型培養装置。   The light irradiation type culture apparatus according to claim 2, wherein the light part dark part alternating means is provided with convex lenses or slits arranged in the vertical direction. 明部暗部交互形成手段を、内筒部材と外筒部材との間に光源を収納する内部空間を備えた中空二重筒構造としてある仕切筒の上記内筒部材と外筒部材の壁面に、水平方向に延びる凸レンズ又はスリットを上下方向に複数配列して設けてなるものとした請求項3記載の光照射型培養装置。   On the wall surfaces of the inner cylinder member and the outer cylinder member of the partition cylinder as a hollow double cylinder structure having an inner space for storing a light source between the inner cylinder member and the outer cylinder member. The light irradiation type culture apparatus according to claim 3, wherein a plurality of convex lenses or slits extending in the horizontal direction are arranged in the vertical direction.
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US10212892B2 (en) 2012-07-10 2019-02-26 Once Innovatians, Inc. Light sources adapted to spectral sensitivity of plant
US10244595B2 (en) 2014-07-21 2019-03-26 Once Innovations, Inc. Photonic engine system for actuating the photosynthetic electron transport chain
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10028448B2 (en) 2012-07-10 2018-07-24 Once Innovations, Inc. Light sources adapted to spectral sensitivity of plants
US10212892B2 (en) 2012-07-10 2019-02-26 Once Innovatians, Inc. Light sources adapted to spectral sensitivity of plant
US10524426B2 (en) 2012-07-10 2020-01-07 Signify Holding B.V. Light sources adapted to spectral sensitivity of plant
US10973173B2 (en) 2012-07-10 2021-04-13 Signify North America Corporation Light sources adapted to spectral sensitivity of plants
US10244595B2 (en) 2014-07-21 2019-03-26 Once Innovations, Inc. Photonic engine system for actuating the photosynthetic electron transport chain
US10813183B2 (en) 2014-07-21 2020-10-20 Signify North America Corporation Photonic engine system for actuating the photosynthetic electron transport chain
KR102029262B1 (en) * 2018-07-12 2019-10-07 주식회사 지디이 Apparatus For Culturing Photoautotroph
JP2020022386A (en) * 2018-08-07 2020-02-13 浜松ホトニクス株式会社 Culture apparatus
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