JP2012527295A - 実験動物から精液を採取する方法およびその人工授精法 - Google Patents
実験動物から精液を採取する方法およびその人工授精法 Download PDFInfo
- Publication number
- JP2012527295A JP2012527295A JP2012511744A JP2012511744A JP2012527295A JP 2012527295 A JP2012527295 A JP 2012527295A JP 2012511744 A JP2012511744 A JP 2012511744A JP 2012511744 A JP2012511744 A JP 2012511744A JP 2012527295 A JP2012527295 A JP 2012527295A
- Authority
- JP
- Japan
- Prior art keywords
- semen
- vas deferens
- artificial insemination
- collecting
- collected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000582 semen Anatomy 0.000 title claims abstract description 86
- 238000000034 method Methods 0.000 title claims abstract description 83
- 230000009027 insemination Effects 0.000 title claims abstract description 38
- 238000010171 animal model Methods 0.000 title claims abstract description 28
- 241001465754 Metazoa Species 0.000 claims abstract description 29
- 210000000918 epididymis Anatomy 0.000 claims abstract description 25
- 201000010063 epididymitis Diseases 0.000 claims abstract description 25
- 210000001177 vas deferen Anatomy 0.000 claims description 47
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 31
- 210000004204 blood vessel Anatomy 0.000 claims description 31
- 210000004369 blood Anatomy 0.000 claims description 26
- 239000008280 blood Substances 0.000 claims description 26
- 239000002504 physiological saline solution Substances 0.000 claims description 15
- 230000002381 testicular Effects 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 13
- 210000003462 vein Anatomy 0.000 claims description 12
- 210000001367 artery Anatomy 0.000 claims description 9
- 230000003187 abdominal effect Effects 0.000 claims description 7
- 210000000702 aorta abdominal Anatomy 0.000 claims description 7
- 241000282472 Canis lupus familiaris Species 0.000 claims description 4
- 241000700159 Rattus Species 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 241000700198 Cavia Species 0.000 claims description 3
- 241000699670 Mus sp. Species 0.000 claims description 3
- 210000001550 testis Anatomy 0.000 abstract description 9
- 230000035935 pregnancy Effects 0.000 abstract description 8
- 210000001215 vagina Anatomy 0.000 description 11
- 210000003754 fetus Anatomy 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 6
- 230000013011 mating Effects 0.000 description 6
- 241000282887 Suidae Species 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 230000005856 abnormality Effects 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 210000002826 placenta Anatomy 0.000 description 4
- 206010043275 Teratogenicity Diseases 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 231100000211 teratogenicity Toxicity 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 230000007758 mating behavior Effects 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000019100 sperm motility Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000283086 Equidae Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000025414 Pentalagus furnessi Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 206010074268 Reproductive toxicity Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 206010042573 Superovulation Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000009408 flooring Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 230000008722 morphological abnormality Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 210000003899 penis Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 210000003689 pubic bone Anatomy 0.000 description 1
- 231100000372 reproductive toxicity Toxicity 0.000 description 1
- 230000007696 reproductive toxicity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/027—Devices for injecting semen into animals, e.g. syringes, guns, probes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/14—Infusion devices, e.g. infusing by gravity; Blood infusion; Accessories therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/26—Inoculator or sampler
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Vascular Medicine (AREA)
- Anesthesiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Animal Husbandry (AREA)
- Surgical Instruments (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (19)
- a)実験動物の輸精管上の外血管を除去する工程;および
b)前記血管が除去された輸精管から精液を採取する工程
を含む、精液採取法。 - 前記工程a)が、前記輸精管の周囲の精巣動静脈を除去することを特徴とする、請求項1に記載の精液採取法。
- 前記工程a)が、前記輸精管と前記精巣動静脈との間に存在する膜に溶媒を注入することを特徴とする、請求項2に記載の精液採取法。
- 前記溶媒が生理食塩水である、請求項3に記載の精液採取法。
- a)実験動物の輸精管上の外血管から血液を除去する工程;および
b)前記血液が除去された輸精管から精液を採取する工程
を含む、精液採取法。 - 前記工程a)が、前記輸精管の周囲の輸精管動静脈から血液を除去することを特徴とする、請求項5に記載の精液採取法。
- 前記工程a)が、精巣動静脈に溶媒を注入することを特徴とする、請求項6に記載の精液採取法。
- 前記溶媒が生理食塩水である、請求項7に記載の精液採取法。
- a)実験動物の腹部大動脈または腹部大静脈を切断して前記腹部大動脈または前記腹部大静脈から血液を除去する工程;および
b)輸精管から精液を採取する工程;
を含む、精液採取法。 - a)実験動物の精巣上体上の外血管を除去する工程;および
b)前記血管が除去された精巣上体から精液を採取する工程;
を含む、精液採取法。 - 前記工程b)が、前記精巣上体を切断して精液を採取することを特徴とする、請求項10に記載の精液採取法。
- 前記工程a)およびb)が、温度30〜45℃を維持することを特徴とする、請求項1〜11のいずれか一項に記載の精液採取法。
- 前記実験動物が、ウサギ、マウス、ラット、イヌ、およびモルモットからなる群から選択されるいずれか1つである、請求項1〜11のいずれか一項に記載の精液採取法。
- 請求項1〜11のいずれか一項に記載の方法により採取された精液を雌に注入することを含む、人工授精法。
- 前記請求項1〜11のいずれか一項に記載の方法により採取された精液が、希釈されて雌に注入されることを特徴とする、請求項14に記載の人工授精法。
- 請求項12に記載の方法により採取された精液を雌に注入することを含む、人工授精法。
- 前記請求項12に記載の方法により採取された精液が希釈されて雌に注入される、請求項14に記載の人工授精法。
- 請求項13に記載の方法により採取された精液を雌に注入することを含む、人工授精法。
- 前記請求項12に記載の方法により採取された精液が希釈されて雌に注入される、請求項18に記載の人工授精法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20090045066 | 2009-05-22 | ||
KR10-2009-0045066 | 2009-05-22 | ||
KR1020090094688A KR101005022B1 (ko) | 2009-05-22 | 2009-10-06 | 실험동물의 정액 채취 방법 및 이를 이용한 인공수정 방법 |
KR10-2009-0094688 | 2009-10-06 | ||
PCT/KR2009/005988 WO2010134672A1 (en) | 2009-05-22 | 2009-10-16 | A method of collecting semen from lab animals and artificial insemination method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012527295A true JP2012527295A (ja) | 2012-11-08 |
JP5087718B2 JP5087718B2 (ja) | 2012-12-05 |
Family
ID=43504165
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012511744A Expired - Fee Related JP5087718B2 (ja) | 2009-05-22 | 2009-10-16 | 実験動物から精液を採取する方法およびその人工授精法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US8678992B2 (ja) |
EP (1) | EP2432419A1 (ja) |
JP (1) | JP5087718B2 (ja) |
KR (1) | KR101005022B1 (ja) |
CN (1) | CN102421389B (ja) |
GB (1) | GB2474184B (ja) |
WO (1) | WO2010134672A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102824228A (zh) * | 2012-09-27 | 2012-12-19 | 天津市力之宝科技有限公司 | 内窥式人工输精仪 |
US10524796B2 (en) | 2014-10-09 | 2020-01-07 | David S. Wilson | Apparatus for irrigating the vas deferens |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003299727A (ja) * | 2002-04-10 | 2003-10-21 | Taiho Yakuhin Kogyo Kk | イヌ類の精液分取装置 |
US20070061910A1 (en) * | 2003-08-08 | 2007-03-15 | Han Jae Y | Method for culturing avian spermatogonial stem cells and avian spermatogonial stem cells prepared thereby |
US20070136830A1 (en) * | 2005-11-09 | 2007-06-14 | Board Of Regents, University Of Texas System | Transgenic rats and spermatogonial stem cells |
JP2009005651A (ja) * | 2007-06-29 | 2009-01-15 | Institute Of Physical & Chemical Research | 精子細胞の保存方法 |
KR20090013543A (ko) * | 2007-08-02 | 2009-02-05 | 건국대학교 산학협력단 | 정조 줄기 세포의 분리방법 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0941310A1 (en) * | 1996-10-11 | 1999-09-15 | THE TEXAS A&M UNIVERSITY SYSTEM | Methods for the generation of primordial germ cells and transgenic animal species |
WO2000040700A2 (en) * | 1999-01-08 | 2000-07-13 | University Of Hawaii | Oocyte activation by sperm components |
WO2004009237A2 (en) * | 2002-07-22 | 2004-01-29 | Xy, Inc. | Sperm cell process system |
CN1611196A (zh) * | 2004-04-14 | 2005-05-04 | 珠江医院 | 简化的双袖套大鼠原位肝移植方法 |
WO2006011693A1 (en) | 2004-07-27 | 2006-02-02 | Jeil Medical Corporation | Bone screw for medical treatments |
KR200408815Y1 (ko) | 2005-10-26 | 2006-02-17 | 주식회사 바이오머테리얼즈코리아 | 치아교정용 임플란트 |
KR100829080B1 (ko) * | 2006-11-24 | 2008-05-19 | 대한민국(관리부서:농촌진흥청) | 뒤영벌 정자채취법 |
-
2009
- 2009-10-06 KR KR1020090094688A patent/KR101005022B1/ko active IP Right Grant
- 2009-10-16 CN CN200980159231.4A patent/CN102421389B/zh active Active
- 2009-10-16 US US13/001,981 patent/US8678992B2/en active Active
- 2009-10-16 EP EP09844980A patent/EP2432419A1/en not_active Withdrawn
- 2009-10-16 WO PCT/KR2009/005988 patent/WO2010134672A1/en active Application Filing
- 2009-10-16 GB GB1101405.7A patent/GB2474184B/en not_active Expired - Fee Related
- 2009-10-16 JP JP2012511744A patent/JP5087718B2/ja not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003299727A (ja) * | 2002-04-10 | 2003-10-21 | Taiho Yakuhin Kogyo Kk | イヌ類の精液分取装置 |
US20070061910A1 (en) * | 2003-08-08 | 2007-03-15 | Han Jae Y | Method for culturing avian spermatogonial stem cells and avian spermatogonial stem cells prepared thereby |
US20070136830A1 (en) * | 2005-11-09 | 2007-06-14 | Board Of Regents, University Of Texas System | Transgenic rats and spermatogonial stem cells |
JP2009005651A (ja) * | 2007-06-29 | 2009-01-15 | Institute Of Physical & Chemical Research | 精子細胞の保存方法 |
KR20090013543A (ko) * | 2007-08-02 | 2009-02-05 | 건국대학교 산학협력단 | 정조 줄기 세포의 분리방법 |
Also Published As
Publication number | Publication date |
---|---|
GB2474184B (en) | 2013-11-27 |
GB2474184A (en) | 2011-04-06 |
WO2010134672A1 (en) | 2010-11-25 |
US20120059215A1 (en) | 2012-03-08 |
GB201101405D0 (en) | 2011-03-16 |
KR101005022B1 (ko) | 2010-12-30 |
JP5087718B2 (ja) | 2012-12-05 |
KR20100126155A (ko) | 2010-12-01 |
CN102421389B (zh) | 2015-03-18 |
EP2432419A1 (en) | 2012-03-28 |
US8678992B2 (en) | 2014-03-25 |
CN102421389A (zh) | 2012-04-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Faigl et al. | Artificial insemination of small ruminants—A review | |
Morrell | Artificial insemination: current and future trends | |
Faizah et al. | Multiple ovulation embryo transfer (MOET) in dairy cattle in Gatton. | |
JP5087718B2 (ja) | 実験動物から精液を採取する方法およびその人工授精法 | |
CN103380770B (zh) | 一种貉精液常温保存稀释液和采精方法 | |
Ombelet et al. | History of human artificial insemination | |
Morrell et al. | Semen handling in South American camelids: state of the art | |
Lindeberg et al. | Potential applications of assisted reproductive technologies (ART) in reindeer (Rangifer tarandus) | |
Vijayalakshmy et al. | Embryo transfer technology in animals: An overview | |
Roberts et al. | Presumed monozygotic twins develop following transfer of an in vitro-produced equine embryo | |
Sharma et al. | An Overview of Artificial Insemination: A Journey from Past to Present | |
Mat et al. | Preliminary study on influence of parity and body condition score (BCS) on estrus response and conception rate of Saanen-cross by estrus synchronization with CIDR | |
KR101047141B1 (ko) | 실험동물의 정액 채취 방법 및 이를 이용한 인공수정 방법 | |
WO2020061612A1 (en) | Method for enhancing assisted breeding techniques | |
ÜSTÜNER et al. | Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons | |
Cholakkal | Artificial insemination in dromedary camels (Camelus dromedarius) using fresh semen extended by physical agitation for achieving liquefaction | |
Dhindsa et al. | Management of Dystocia Caused by Pygodidymus Monster in Crossbred Cattle | |
Vaughan | Artificial Breeding in alpacas | |
Perera et al. | Establishment of multiple ovulation and embryo transfer (MOET) technology for goats in Sri Lanka | |
Leigh et al. | Conception following transcervical insemination with predetermined ejaculate concentration in synchronized West African dwarf does | |
Faria et al. | Fixed-time artificial insemination in the bovine'Lydia Brava dos Açores' using frozen semen. | |
Niżański | Can we use reproductive biotechniques in cats like it is used in dogs? | |
Prasath | A case report of unusual dystocia relief: Triplets in a single birth with breech presentation in a Holstein Friesian crossbreed cattle | |
Hasan Riaz et al. | Dystocia in blackbucks (Antilope cervicapra) | |
Boediono | Preservation of garut rams spermatozoon as a source of male germ plasm |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20120814 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20120910 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5087718 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150914 Year of fee payment: 3 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |