JP2012523832A - 種子生産性が安定化された高妊性ラパノブラシカ新品種植物 - Google Patents
種子生産性が安定化された高妊性ラパノブラシカ新品種植物 Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/026—Methods or apparatus for hybridisation; Artificial pollination ; Fertility by treatment with chemicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
- A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/138—Amplified fragment length polymorphism [AFLP]
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Abstract
【選択図】図1
Description
新系統のラパノブラシカ新品種植物の育種方法で小胞子突然変異法を用いようにした。本発明者などによって開発された小胞子培養では胚発生率が培養皿当たり0.9個しかならないので(洪と李、1995)突然変異誘導手段で適合しない状態だ。したがってもう少し胚発生量が多い培養法を確立することと同時に突然変異剤NMUを0.01μg/Lで処理する試験を遂行した。多くの試験中胚がたくさん出るある一つの試験で由来した胚を植物に誘導して後代を採種した。その結果従来のラパノブラシカより室内人工交配で採種量が多い個体があった(農林部課題(ARPC)、"新野菜作物ラパノブラシカの主要形質改善及び安さ野菜で年中生産研究"の最終報告書(2006)、31-38ページの表15-23参照)。
均一性及び種子生産性確認評価
種子生産能力を確認するための室内交配試験でやはり突然変異剤が処理された系統は前年度と同じように種子がたくさん収穫された(表1)。
新系統のラパノブラシカ新品種植物の育種方法で小胞子突然変異法を用いようにした。本発明者などによって開発された小胞子培養では胚発生率が培養皿当たり0.9個しかならないので(洪と李、1995)突然変異誘導手段で適合しない状態だ。したがってもう少し胚発生量が多い培養法を確立することと同時に突然変異剤NMUを0.01μg/Lで処理する試験を遂行した。多くの試験中胚がたくさん出るある一つの試験で由来した胚を植物に誘導して後代を採種した。その結果従来のラパノブラシカより室内人工交配で採種量が多い個体があった(農林部課題(ARPC)、"新野菜作物ラパノブラシカの主要形質改善及び安さ野菜で年中生産研究"の最終報告書(2006)、31-38ページの表15-23参照)。NMU(n-nitron-methy1 urethane)は、分子量が132として半減期がはやくて光によって分解されるから小胞子培養直前に製造して処理する。すなわち、小胞子が培養液1 ml当たり100,000個入っている溶液を先に作る。そして直ちに電灯が消えたクリーンベンチ内で下のような過程でNMU濃度を薄めて0.01μmなるように培養液に添加する。そして培養液を2.5 mlずつ直径60mmpetridishに奔走して普通の小胞子培養過程に準じて培養する。すなわち処理中にNMUを洗い落とさないでそのまま置いて培養することである。
NMU濃度希薄過程は、
- 1M NMU=132 ml NMU/L H 2 O
- 10Mm(X100希薄)溶液製造=1.32ml/L
∴1.32ml = 132μ 1 = 132p/100ml H 2 O
- 0.1Mm(X100希薄)溶液製造=10Mm溶液1ml/100mL H 2 O
- 1.0mM(X希薄)溶液製造=0.1mM溶液1ml/100mL H 2 O
培養液100mLに0.1μM NMU溶液1mlを混合して0.01μM濃度で調整して使った。
均一性及び種子生産性確認評価
種子生産能力を確認するための室内交配試験でやはり突然変異剤が処理された系統は前年度と同じように種子がたくさん収穫された(表1)。
Claims (1)
- 突然変異誘発物質NMUを0.01μg/L処理してラパノブラシカ種子を突然変異処理する段階と;種まきの後採種する段階と;室内人工交配で種子生産を確認する段階と;AFLPプライマー(primer)を利用して品種の均一性を確認する段階を結合したことが特徴の種子生産性が安定化された高妊性ラパノブラシカ新品種植物の育種方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2009-0055969 | 2009-06-23 | ||
KR20090055969 | 2009-06-23 | ||
PCT/KR2010/001568 WO2010150968A2 (ko) | 2009-06-23 | 2010-03-12 | 종자생산성이 안정화된 고임성 배무채 신품종 식물 |
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JP2012523832A true JP2012523832A (ja) | 2012-10-11 |
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JP2012505803A Pending JP2012523832A (ja) | 2009-06-23 | 2010-03-12 | 種子生産性が安定化された高妊性ラパノブラシカ新品種植物 |
Country Status (6)
Country | Link |
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US (1) | US20120210457A1 (ja) |
EP (1) | EP2446735A4 (ja) |
JP (1) | JP2012523832A (ja) |
KR (1) | KR101328182B1 (ja) |
CN (1) | CN102427718A (ja) |
WO (1) | WO2010150968A2 (ja) |
Families Citing this family (1)
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CN113615571A (zh) * | 2021-09-16 | 2021-11-09 | 山西省农业科学院作物科学研究所 | 一种小麦诱变育种方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004057947A1 (en) * | 2002-12-30 | 2004-07-15 | Soo-Sung Lee | A new species of plant baemoochae and method for breeding the same |
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2010
- 2010-03-12 CN CN2010800179967A patent/CN102427718A/zh active Pending
- 2010-03-12 JP JP2012505803A patent/JP2012523832A/ja active Pending
- 2010-03-12 EP EP10792250A patent/EP2446735A4/en not_active Withdrawn
- 2010-03-12 US US13/265,373 patent/US20120210457A1/en not_active Abandoned
- 2010-03-12 WO PCT/KR2010/001568 patent/WO2010150968A2/ko active Application Filing
- 2010-06-21 KR KR1020100058451A patent/KR101328182B1/ko active IP Right Grant
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004057947A1 (en) * | 2002-12-30 | 2004-07-15 | Soo-Sung Lee | A new species of plant baemoochae and method for breeding the same |
Non-Patent Citations (2)
Title |
---|
JPN6013032933; 'TILLING to detect induced mutations in soybean' BMC Plant Biology, (2008), Vol. 8 9 * |
JPN6013032934; Theor. Appl. Genet., (2001), Vol. 103, p. 1254-1265 * |
Also Published As
Publication number | Publication date |
---|---|
CN102427718A (zh) | 2012-04-25 |
WO2010150968A2 (ko) | 2010-12-29 |
KR101328182B1 (ko) | 2013-11-19 |
EP2446735A4 (en) | 2013-03-20 |
EP2446735A2 (en) | 2012-05-02 |
US20120210457A1 (en) | 2012-08-16 |
WO2010150968A3 (ko) | 2011-02-17 |
KR20100138778A (ko) | 2010-12-31 |
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