JP2012254065A - Method for collecting microorganism - Google Patents

Method for collecting microorganism Download PDF

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JP2012254065A
JP2012254065A JP2011141168A JP2011141168A JP2012254065A JP 2012254065 A JP2012254065 A JP 2012254065A JP 2011141168 A JP2011141168 A JP 2011141168A JP 2011141168 A JP2011141168 A JP 2011141168A JP 2012254065 A JP2012254065 A JP 2012254065A
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pin
collection
sampling
collecting pin
collecting
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Tokuji Osada
徳治 長田
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract

PROBLEM TO BE SOLVED: To collect microorganisms from constantly the same depth even when the thickness of a medium varies in the case where the microorganisms are collected by sticking a collecting pin into the medium.SOLUTION: There is provided a method using the collecting pin in which a leading end portion formed at a lower end is retained so as to be capable of inserting/removing into/from a breeding ground, and a driving mechanism of a collecting pin in which the collecting pin is slidably supported in a vertical axis direction and the collecting pin is driven up and down in the vertical axis direction while retaining the collecting pin at the bottom position of a sliding process in a free state. The method includes, after the collecting pin is moved down by the driving mechanism of the collecting pin and the collecting pin is stuck into a breeding ground of an incubator by its self load, collecting microorganisms from the breeding ground by moving up the collecting pin by the driving mechanism of the collecting pin and removing the collecting pin from the breeding ground.

Description

本発明は、培養地からの微生物採取方法に関し、詳しくは培養器の培養地からＸＹＺ駆動機構を用いて微生物を採取する際のＺ軸の支持方法に関する。  The present invention relates to a method for collecting microorganisms from a culture site, and more particularly, to a method for supporting a Z-axis when collecting microorganisms from a culture site of an incubator using an XYZ drive mechanism.

従来、複数の培養地から順次微生物を採取する場合は、特許文献１に記載されているようなＸＹＺテーブルを用いることがある。ＸＹＺテーブルを用いた場合に、ＸＹ位置（平面位置）を変えながら複数の培養器から微生物を採取するには、異なる培養器ごとにＺ軸（垂直軸）方向に採取ピンを昇降させて採取ピンを培養地に突き刺して微生物を採取していた。このとき採取ピンの下端位置は、通常毎回同じであるため、培養器ごとの培養地の上面レベルが毎回同じであれば常に同一条件つまり同一深さまで採取ピンを突き刺して微生物のサンプリングが行える。  Conventionally, when sequentially collecting microorganisms from a plurality of culture sites, an XYZ table as described in Patent Document 1 may be used. To collect microorganisms from multiple incubators while changing the XY position (planar position) when using an XYZ table, the collection pins are moved up and down in the Z-axis (vertical axis) direction for each different incubator. The microorganism was collected by piercing the culture medium. At this time, since the lower end position of the collecting pin is usually the same every time, if the upper surface level of the culture place for each incubator is the same every time, the sampling pin can always be stabbed to the same condition, that is, the same depth, and the microorganism can be sampled.

特開２０００−２３２８７３号公報  JP 2000-232873 A

しかしながら、培養器ごとに培養地の上面レベルにばらつきがある場合は、そのレベルの高低に応じて突き刺される採取ピンの深さが変化して同一条件でのサンプリングにはならないという問題があった。
そこで、本発明は、ＸＹ位置（平面位置）を変えながらＺ軸（垂直軸）方向に採取ピンを昇降させて複数の培養器から順番に微生物を採取する場合に、培養器ごとに培養地の表面レベルが変化しても、常に同一圧力で採取ピンを培養地に突き刺すことにより、ほぼ毎回一定深さまで採取ピンを突き刺してサンプリングすることができる微生物採取方法を提案することを目的とした。However, when there is a variation in the upper surface level of the culture place for each incubator, there is a problem that the sampling pin depth to be stabbed changes depending on the level, and sampling under the same conditions is not possible.
Therefore, in the present invention, when microorganisms are collected in order from a plurality of incubators by moving up and down the collection pin in the Z-axis (vertical axis) direction while changing the XY position (planar position), the culture site of each incubator is changed. An object of the present invention is to propose a method of collecting microorganisms that can be sampled by piercing the sampling pin to a certain depth almost every time by always piercing the culture medium with the same pressure even when the surface level changes.

上記課題を解決するために、本発明は、下端に形成された先端部が培養地に抜き挿し自在に保持された採取ピンと、該採取ピンを垂直軸方向滑動自在に支持するとともにフリー状態では採取ピンを滑動行程最下位に保持しながら採取ピンを垂直軸方向に昇降駆動する採取ピン駆動機構とを備え、前記採取ピンを前記採取ピン駆動機構により下降させ、培養器の培養地に前記採取ピンをその自重荷重で突き刺した後、前記採取ピンを前記採取ピン駆動機構により上昇させて前記培養地から前記採取ピンを抜き取ることにより培養地から微生物を採取することを特徴とした。  In order to solve the above-mentioned problems, the present invention provides a sampling pin whose tip formed at the lower end is detachably held in a culture medium, and supports the sampling pin so as to be slidable in the vertical axis direction and is sampled in a free state. A collection pin drive mechanism that drives the collection pin to move up and down in the vertical axis direction while holding the pin at the lowest position in the sliding stroke, and the collection pin is lowered by the collection pin drive mechanism, and the collection pin is placed in a culture place of an incubator , The microorganism is collected from the culture site by raising the collection pin by the collection pin drive mechanism and extracting the collection pin from the culture site.

また、本発明は、上記の微生物採取方法において、前記採取ピンと前記採取ピン駆動機構との間に螺旋溝機構を形成して、前記採取ピンが前記採取ピン駆動機構に対して相対的に上昇する際に前記採取ピンに回転運動を発生させることを特徴とした。  Further, the present invention provides the above-described microorganism collection method, wherein a spiral groove mechanism is formed between the collection pin and the collection pin drive mechanism, and the collection pin is raised relative to the collection pin drive mechanism. In this case, a rotational motion is generated in the sampling pin.

さらに、本発明は、上記の微生物採取方法において、前記採取ピンに着脱自在に錘を取り付けたことを特徴とした。  Furthermore, the present invention is characterized in that, in the microorganism collection method, a weight is detachably attached to the collection pin.

以上述べたように本発明によれば、採取ピンが垂直軸方向滑動自在に支持されたことにより、採取ピンはその自重により培養地に突き刺されることになり、培養器ごとに培養地の表面レベルが変化しても、常に同一圧力で採取ピンが培養地に突き刺ささり、ほぼ毎回一定深さまで採取ピンを突き刺してサンプリングすることができる。  As described above, according to the present invention, since the collection pin is supported so as to be slidable in the vertical axis direction, the collection pin is inserted into the culture place by its own weight, and the surface level of the culture place is determined for each incubator. Even if changes, the sampling pin always pierces the culture medium with the same pressure, and sampling can be performed by piercing the sampling pin to a constant depth almost every time.

以下、図に基づいて本発明の実施形態を説明する。図１は本発明に係る微生物採取方法に用いられる採取ピンの構成を示す説明図であり、左側が待機時を、右側が接地時を示している。図２は本発明に係る微生物採取方法に用いられる採取ピンの他の構成を示す説明図である。  Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is an explanatory diagram showing the configuration of a collection pin used in the microorganism collection method according to the present invention, with the left side showing standby and the right side showing grounding. FIG. 2 is an explanatory view showing another configuration of the collection pin used in the microorganism collection method according to the present invention.

図１の左側では、採取ピンであるところのピンが矯正板１、２に水平方向の移動を規制されて支持されるとともに昇降可能に支えられている。図１の右側の接地時はピンが培地に接触している。ピンは矯正板１、２により水平方向のみ支えられていて上下方向の抜き差しが可能であるため、取り替えが簡単に出来る。又ピンが上下すると回転機構によりピンは回転する。図１では、回転機構として、ピン側にリードの大きい螺旋溝または螺旋凸条を形成するとともに、矯正板２側に該螺旋溝または該螺旋凸条に嵌合する突起または溝を形成している。それにより、ピンが下降して下端が培地に突き当たると、ピンが矯正板２に対して相対的に上昇しながら回転駆動されて、ピン下端が捻じれながら培地に突き刺される。  On the left side of FIG. 1, a pin that is a sampling pin is supported by the correction plates 1 and 2 so that the movement in the horizontal direction is restricted and supported so as to be movable up and down. At the time of grounding on the right side of FIG. 1, the pin is in contact with the medium. Since the pins are supported only in the horizontal direction by the correction plates 1 and 2 and can be inserted and removed in the vertical direction, replacement can be easily performed. When the pin moves up and down, the pin is rotated by the rotation mechanism. In FIG. 1, as a rotation mechanism, a spiral groove or a spiral ridge having a large lead is formed on the pin side, and a protrusion or groove that fits the spiral groove or the spiral ridge is formed on the correction plate 2 side. . Thereby, when the pin descends and the lower end hits the medium, the pin is driven to rotate while being raised relative to the correction plate 2, and the lower end of the pin is twisted and pierced into the medium.

これらの構成により、接地時すなわちピンが培地に当接しているときは培地にかかる荷重はピンの自重のみとなる。すなわち、培地の厚みが変化しても、ピンの培地への喰い込み深さが毎回略一定となり、常に同じ深さで培地から菌等の微生物が採取できる。  With these configurations, the load applied to the culture medium is only the weight of the pin when grounded, that is, when the pin is in contact with the culture medium. That is, even if the thickness of the medium changes, the biting depth of the pin into the medium becomes almost constant every time, and microorganisms such as bacteria can be collected from the medium at the same depth.

図２では、筒状の収納体に上下方向抜き差し自在にピンが収納されているとともに、ピンの上端に追加可能な錘がネジ固定されている。これはピンを深く突き刺したい場合や、培地が固い場合に、錘を追加可能にするものである。  In FIG. 2, a pin is housed in a cylindrical housing body so that it can be inserted and removed in the vertical direction, and a weight that can be added is screwed to the upper end of the pin. This makes it possible to add a weight when it is necessary to pierce the pin deeply or when the medium is hard.

本発明に係る微生物採取方法に用いられる採取ピンの構成を示す説明図であり、左側が待機時を、右側が接地時を示している。It is explanatory drawing which shows the structure of the collection | recovery pin used for the microorganisms collection method which concerns on this invention, The left side has shown the time of standby, and the right side has shown the time of grounding. 本発明に係る微生物採取方法に用いられる採取ピンの他の構成を示す説明図である。It is explanatory drawing which shows the other structure of the collection pin used for the microorganisms collection method which concerns on this invention.

１ 矯正板
２ 矯正板1 Correction plate 2 Correction plate

Claims (3)

下端に形成された先端部が培養地に抜き挿し自在に保持された採取ピンと、該採取ピンを垂直軸方向滑動自在に支持するとともにフリー状態では採取ピンを滑動行程最下位に保持しながら採取ピンを垂直軸方向に昇降駆動する採取ピン駆動機構とを備え、
前記採取ピンを前記採取ピン駆動機構により下降させ、培養器の培養地に前記採取ピンをその自重荷重で突き刺した後、前記採取ピンを前記採取ピン駆動機構により上昇させて前記培養地から前記採取ピンを抜き取ることにより培養地から微生物を採取することを特徴とした微生物採取方法。A sampling pin whose tip formed at the lower end is detachably held in the culture medium, and the sampling pin is slidably supported in the vertical axis direction, and in the free state, the sampling pin is held at the bottom of the sliding stroke. And a sampling pin drive mechanism for driving up and down in the vertical axis direction,
The collection pin is lowered by the collection pin drive mechanism, and the collection pin is pierced into the culture place of the incubator with its own weight, and then the collection pin is raised by the collection pin drive mechanism to collect the collection pin from the culture place. A method for collecting microorganisms, characterized in that microorganisms are collected from a culture site by extracting a pin. 請求項１に記載の微生物採取方法において、
前記採取ピンと前記採取ピン駆動機構との間に螺旋溝機構を形成して、前記採取ピンが前記採取ピン駆動機構に対して相対的に上昇する際に前記採取ピンに回転運動を発生させることを特徴とした微生物採取方法。The method for collecting microorganisms according to claim 1,
Forming a spiral groove mechanism between the sampling pin and the sampling pin driving mechanism, and generating a rotational motion of the sampling pin when the sampling pin is raised relative to the sampling pin driving mechanism; Characteristic microorganism collection method. 請求項１または２に記載の微生物採取方法において、
前記採取ピンに着脱自在に錘を取り付けたことを特徴とした微生物採取方法。In the microorganism collection method according to claim 1 or 2,
A method for collecting microorganisms, wherein a weight is detachably attached to the collection pin.
JP2011141168A 2011-06-09 2011-06-09 Method for collecting microorganism Withdrawn JP2012254065A (en)

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JP2011141168A JP2012254065A (en) 2011-06-09 2011-06-09 Method for collecting microorganism

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017061387A1 (en) * 2015-10-07 2017-04-13 学校法人早稲田大学 Sample collection system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017061387A1 (en) * 2015-10-07 2017-04-13 学校法人早稲田大学 Sample collection system
CN108138109A (en) * 2015-10-07 2018-06-08 前沿生物系统有限公司 Sampling system
JPWO2017061387A1 (en) * 2015-10-07 2018-08-09 フロンティアバイオシステムズ株式会社 Sampling system

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