JP2012105594A - Method for raising cutting slip mother tree of eucalyptus globulus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an efficient clonal proliferation technique of an Eucalyptus globulus.SOLUTION: In the method for raising the cutting slip mother tree of the Eucalyptus globulus, a plant growth regulator including paclobutrazole as an active ingredient is sprayed in soil 1.25 mg or more of the active ingredient amount per litter of the ridging amount of the cutting slip mother tree.

Description

  The present invention relates to a method for raising seedlings of Eucalyptus globulus cuttings, which can maximize the rooting rate from cuttings.

  The agents currently used for plant growth regulation are plant growth regulators, such as plant rooting / establishment promotion, healthy seedling, prevention of plant damage, side bud development, bud bud suppression, flowering / fruit set, hypertrophy, Its effects include coloring, maturity promotion, elongation inhibition (hatching), fall prevention, fruit removal, rust prevention, chemical damage prevention, and healing promotion. Among these, as plant growth regulators used for plant elongation inhibition (hatching), ansimidol, dikeglac, daminogit, mefluidide, chlormecote, paclobutrazole, inabenfide, etc. It contributes to the improvement of crop yield and product quality. Its use is limited to flower buds, garden trees, fruit trees, turf, wheat, and rice, and it is a medicinal ingredient that depends on the concentration used, so knowledge and experience are required to select methods for avoiding chemical damage and when to use it. .

  For plants, the roots have a variety of roots, such as absorbing moisture and nutrients necessary for growth, supporting the ground and preventing lodging, and it has been pointed out that it is important to fully develop plant roots in the agricultural field. (For example, see Non-Patent Documents 1 and 2).

  However, plant growth regulators that promote root development have a small number, are not sufficiently effective, and often have undesirable effects. For example, auxin compounds that are widely used as rooting agents at present are induced by epidermal bending, stem twisting and cracking, and root nodule, depending on the type and condition of the plant and the concentration applied. Furthermore, it may exert an undesirable effect such as death. For this reason, the method of use, the amount of use, etc. are limited, and the action of promoting root development has not been fully satisfactory.

  Paclobutrazol, a kind of plant growth regulator with bactericidal activity, inhibits gibberellin biosynthesis in the plant body, and has the effect of suppressing cell elongation, that is, plant height elongation, which is the main action of gibberellin. It is used to hatch rice, fruit trees, horticultural crops, reduce lodging.

  By the way, Eucalyptus globulus grows in the Oceania region, has excellent growth potential, and in addition, has high bulk weight and pulp yield, is industrially suitable for wood production and pulpwood, and is planted around the world. . Producing clonal seedlings with excellent traits (growth, tree shape, etc.) of this Eucalyptus globulus is an industrial advantage, and there is a global demand for techniques for efficient clonal propagation.

  Therefore, cutting rooting ability has a great influence on the productivity of cloned seedlings, and its improvement is an important issue. Various methods for using growth regulators have been studied, methods for efficient seedling management using hatching techniques using strains (see, for example, Patent Document 1), plant flower bud formation promoters, or dormancy breaking, rooting, Provision of α-ketol unsaturated fatty acid derivatives as plant growth regulators including uses such as plant activators that exhibit performance such as flowering promotion, flowering promotion, grass / stem elongation promotion, etc. (see, for example, Patent Document 2), lactones Derivatives, and plant growth regulators and rooting inducers comprising the same as active ingredients (see, for example, Patent Document 3), amide derivatives having high plant rooting promoting activity and substantially no side effects, and Providing a plant growth regulator comprising an amide derivative as an active ingredient (for example, see Patent Document 4), and producing a plant growth regulator by mixing auxin, cytokinin, peptide, and sildenafil citrate Fine Usage (e.g., see Patent Document 5), and the like.

  In addition, there are some that are considering using inhibitors of the gibberellin biosynthetic pathway for cutting plant seedling production of Eucalyptus plants and Acacia plants (see, for example, Patent Document 6). The cuttings of peach and olives are treated with paclobutrazol in advance, and then the cuttings are collected and treated with indole butyric acid (IBA), a rooting promoter, before the collected cuttings are inserted. It is known that the survival rate and rooting rate are improved when inserted from (see, for example, Non-Patent Documents 3 and 4).

  However, none of these findings aim to improve the rooting rate by grasping the growth characteristics of tree species and correlating with the sustained effect of plant growth regulators. Although the improvement of rooting rate is recognized by the addition of paclobutrazol, etc., the point that the rooting rate is most improved varies depending on the plant species, and even if it changes within the same genus, The effectiveness of the effect also changes. Even if we consider improving the rooting rate without paying attention to the growth potential of the plant body after taking the drug to the plant and cutting it, it is optimal for the maximum improvement of the rooting rate to Eucalyptus globulus There is a problem that conditions cannot be found.

JP-A-10-287520 JP 2006-151830 A JP 2003-055366 A JP 2001-139405 A JP 2001-058912 A JP 2001-231355 A

Edited by Boote et al., Physiology and Determination of Crop Yield, p. 65-93, 1994 Root Encyclopedia Editorial Committee, Root Encyclopedia, p. 261-289, 1998 Wiesman, Z .; Riov, J .; Epistein, E .; Hort Science, 24 (6), p. 908-909, 1989 Wiesman, Z .; Lavee, S .; , Plant Growth Regulation 14, p. 83-90, 1994

  When the growth adjustment treatment for the plant is performed, it is necessary to perform the treatment several times while watching the growth of the plant. Therefore, a lot of labor is required, and the growth adjustment cannot be easily performed. In addition, since a large amount of cutting material is required when performing clonal propagation with cuttings, if the hatching process is performed in the early stage of growth, the material that can be collected decreases. That is, hatching processing after growing up to a certain size is required.

  Since the type and concentration of the plant growth regulator vary depending on the purpose of the treatment and the type of plant, it is necessary to use a plant growth regulator and concentration suitable for the plant. When growing, the correlation between the growth inhibition of the material and the improvement of the rooting rate is different in the use method of the chemicals if the types are different even among plants or plants in the same genus.

  An object of the present invention is to provide a growth technique of a mother tree for growing a clone seedling of Eucalyptus globulus, which is frequently used as a fast-growing species of pulpwood, using a growth regulator. Therefore, the cutting rooting rate is improved.

  The present inventors have optimized the time of picking from the time of hatching treatment to Eucalyptus globulus, and as a result of intensive studies, focused on the amount of drug, the seedling period after hatching treatment, and the growth of the mother tree Thus, the optimum point at which the rooting rate was most improved was found under the correlation with the growth of the seedling, and the present invention was completed.

  The present invention is a eucalyptus globula cutting characterized in that it is applied to the soil so that the amount of paclobutrazol, which is an active ingredient in the plant growth regulator, is 1.25 mg or more per liter of soil of the cuttings harvested from the head of the cutting. This is a method for growing a harvesting mother tree. It is preferable to set the seedling raising period of the cutting tree harvesting mother tree after spraying the plant growth regulator to the cuttings within one month, or a period in which the growth of the cutting tree harvesting mother tree is suppressed to within 20 cm. Moreover, it is preferable that the height of a cutting cutting head mother tree is 90-140 cm.

  According to the present invention, the rooting ability can be maximally promoted by specifying the amount of active ingredient (paclobutrazol) with respect to the cutting cutting head tree. Further, by specifying the height of the cutting cutting heading mother tree and the seedling raising period after adding the chemical, the rooting rate can be further improved, and efficient seedling production becomes possible.

  The use of the plant growth regulator of the present invention is not mainly intended for the hatching of plants as its effect, but limited to the problem of eucalyptus globulus clonal propagation, and the effect of suppressing plant tree height We focus on searching for the optimum conditions for improving the rooting rate and improving productivity while obtaining the correlation between sustainability and rooting ability. Hereinafter, the present invention will be described in detail.

  As the form of the cutting tree harvesting mother tree for collecting the cuttings of Eucalyptus globulus, any of pot seedlings and open-planted seedlings may be used, and those of natural tree shape are preferred rather than those pruned to grow sprout branches . It is necessary to measure the growth of the tree height and the growth of the buds as an index of the hatching effect when the hatching treatment is performed. When measuring the elongation of the latter, the selected one is measured. This is because the number increases and is complicated.

  As a type of cutting cutting head mother tree, not only a seedling seedling but also a cloned seedling in which a parent tree is rejuvenated by a method such as cutting, grafting, or tissue culture may be used. The age of the cutting cutting head mother tree is not particularly limited, but the younger one is preferable because it has a high rooting ability of cuttings.

  Next, a method for treating a cutting cutting head tree with a plant growth regulator in advance will be described. Examples of the method of treating with a plant growth regulator include a method of treating the surrounding soil of the cutting heading tree with a granule or a compound fertilizer, and a method of treating the surrounding soil of the cutting heading tree with a wettable powder. The treatment time is more preferable when the shoots of the plant before summer start to grow and grow, but is not particularly limited as long as the plant grows.

  As the types of plant growth regulators, inhibitors of gibberellin biosynthetic pathways can be broadly divided into triazoles (paclobutrazole, uniconazole), pyrimidines (anthimidol, flurprimidol), isonicotinamide (inabenfide). ) Quaternary amine (chlormequat) and others (daminozide, prohexadione). In particular, triazole-based paclobutrazol is preferable as a type frequently used to promote rooting, and this is used in the present invention.

  The amount of the plant growth regulator varies depending on the drug used and cannot be generally stated, but it may be in accordance with the usage amount used for elongation inhibition (hatching) by internode elongation regulation, which is the conventional purpose of each drug. . However, since the effect of the effect is a correlation with the height and growth of the cutting cutting headstock, it is necessary to examine the amount of the reagent in accordance with the growth characteristics of the target plant. In the present invention, an amount of 1.25 mg or more of a plant growth regulator containing paclobutrazol as an active ingredient is sprayed on the soil with respect to 1 L of the soil volume of cutting cuttings. If the amount of the plant growth regulator containing paclobutrazol is less than 1.25 mg, the plant will hatch, but the rooting rate will not be improved in difficult rooting species. Moreover, a preferable upper limit is 6.25 mg or less because it is within the range where no phytotoxicity occurs and the rooting is reliably improved.

  Eucalyptus globulae treated with a plant growth regulator is hatched with elongation growth suppressed from about 2 weeks after treatment. Hatching shows morphological changes such as shorter internodes and smaller leaves. Thus, the cuttings that have undergone morphological changes are suitable for cutting and seedling production. Therefore, it is preferable to raise seedlings for 2 weeks or more after the application of the plant growth regulator to the soil.

  It is preferable that the seedling raising period of the cutting heading mother tree after the plant application of the plant growth regulator to the cutting is within one month. By setting it within one month, cuttings can be executed with short-term mother tree management, and after cutting, the same cutting tree harvesting mother tree can be used to quickly grow the branch that is the next cutting material.

  Further, as another guideline, it is preferable to set the growing period of the cutting heading mother tree after the plant application of the plant growth regulator to the cutting operation as a period in which the growth of the cutting heading mother tree is suppressed within 20 cm. When the elongation of the cutting cutting heading tree exceeds 20 cm, there may be a problem that the rooting rate decreases. In addition, if the plant body is too hatched, it is preferable that the growth length of the cutting tree cutting mother tree is 10 cm or more because a large amount of cuttings as a material cannot be secured.

  The height of the cutting cutting heading tree is preferably 90 to 140 cm, more preferably 95 cm to 130 cm, and still more preferably 100 to 120 cm. If the height of the cutting tree harvesting mother tree is less than 90 cm, the absolute number of cutting material obtained from the mother tree will be reduced, and short branches between nodes will be created, and the efficiency of cutting work will be reduced. May arise. Moreover, when it exceeds 140 cm, depending on the amount to be added, there may be a problem that the effect of hatching is not seen and rooting improvement cannot be confirmed.

  Next, a method of growing by inserting the cuttings collected from the cutting cutting mother tree whose growth has been adjusted as described above into cutting cutting medium will be described. The cuttings used for the cuttings are selected from the young branches up to the current branch or the side branches generated from the branches. Immediately immerse the collected branches in the prepared water. The collected branches or side branches are cut into lengths for each node with two leaves attached, and this is used as a spike. After soaking in a disinfectant, a hormonal agent such as indolebutyric acid (IBA) is applied to the cutting base of the cutting head and inserted into the cutting medium.

  As an insertion floor, in addition to commercially available artificial soil (Oasis (registered trademark), rock wool, etc.), common culture soil such as red soil, Kanuma soil, peat moss, vermiculite, perlite, etc. alone or appropriately mixed Can be used. The cuttings are irrigated once or twice a day so that they do not become too dry, and are allowed to stand for 6 to 8 weeks.

  Hereinafter, the present invention will be described in more detail with reference to examples.

(Experimental example 1)
Among the Eucalyptus globulus clone seedlings, Sumarect (registered trademark) granules (0.6% active ingredient) were added to the parent tree clone A with a tree height of 100 to 110 cm, the mother tree clone B with a tree height of 100 to 120 cm, and the mother tree clone C with a tree height of 100 to 120 cm ; Manufactured by Ishihara Sangyo Co., Ltd.) was dispersed and sprayed on the surface of the cultured soil so that the amount of the active ingredient was 1.25 mg and 6.25 mg in 1 L of the cultured soil. Cuttings were prepared at 4 weeks after soil application, and cuttings with a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Three strains were used in one experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 1 shows the results of tree height growth and rooting rate after 4 weeks of soil application.

  As shown in Table 1, in clones A, B, and C, compared to untreated, in cutting cutting head mother tree sprayed with a reagent having a hatching active ingredient amount of 1.25 mg or more in 1 L of soil, The elongation was suppressed to about 10 to 20 cm, whereas the elongation of the untreated mother tree was about 30 to 50 cm. At the same time, the cuttings obtained from the hatched mother tree showed a high rooting rate compared to the mother tree not treated with the drug.

  When the height of the mother tree to which the chemical is sprayed is 100 to 120 cm, the amount of the hatching active ingredient in 1 L of soil is 1.25 mg or more, and within one month after spraying, or the growth period of cutting cutting head tree is within 20 cm, sufficient It was confirmed that the rooting rate of the cuttings collected from the cuttings that had been hatched and reproduced the situation was the highest.

(Experimental example 2)
Sumalect (registered trademark) granule (active ingredient 0.6%; manufactured by Ishihara Sangyo) is cultured using the cutting tree harvesting parent tree clone A with a tree height of 90 cm to 140 cm among Eucalyptus globulus clone pot seedlings grown on cuttings In 1 L of soil, the active ingredient was dispersed and sprayed on the surface of the pot culture soil so that the amount of the active ingredient was 6.25 mg. At the time of 2 weeks, 4 weeks, 8 weeks, and 12 weeks after the treatment, cuttings were prepared, and cuttings having a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Three strains were used in one experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 2 shows the results of tree height growth and rooting rate in each treatment period.

  As shown in Table 2, although the growth of some tree heights was suppressed in the treated group for 2 weeks after raising compared to the untreated group, the rooting rate was also slightly improved. In the seedling growing period of 8 weeks to 12 weeks, the growth of the tree height was about 25 cm or more, and the cuttings obtained from these cuttings were not confirmed to improve the rooting rate as compared to the untreated. On the other hand, in the seedling period of 4 weeks, the growth of the tree height was suppressed from about 14 cm to 19 cm, within 20 cm, and it was confirmed that the rooting rate was greatly improved.

(Experimental example 3)
Among the clone pot seedlings of Eucalyptus globulus grown on cuttings, Sumalect (registered trademark) granule (active ingredient 0.6%; Ishihara Sangyo) is a paclobutrazol preparation using a parent tree clone D having a tree height of 90 to 110 cm (Made) Each amount of 1, 2, 3, 5 g was scattered and spread on the surface of the pot soil. The amount of active ingredients per liter of soil is 0.25 mg, 0.50 mg, 0.75 mg, and 1.25 mg.

  At the time of 1 month after the treatment, cuttings with a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Three strains were used in one experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 3 shows the results of tree height growth and rooting rate during one month after treatment.

  As shown in Table 3, the amount of active ingredient in 1 L of soil is 0.25 mg treated, and compared to the untreated plot, the growth of tree height is suppressed and a slight hatching effect is observed, but the rooting rate is improved. Was not confirmed. On the other hand, no hatching effect was observed in the 0.50 mg treated group and the 0.75 mg treated group, and no improvement in the rooting rate was confirmed. On the other hand, in the 1.25 mg treatment section, the growth of the cutting cutting heading tree was 20 cm or less, the hatching effect was confirmed, and the rooting rate was improved. That is, a sufficient hatching effect can be obtained by using an optimal reagent amount of 1.25 mg or more of the active ingredient of paclobutrazol in 1 L of soil for the height of the cutting cutting heading tree. It was confirmed that the rooting rate was improved.

  The present invention relates to a method for growing Eucalyptus globulus, and relates to a method for clonal propagation and seedling with high efficiency.

As the form of the cutting tree harvesting mother tree for collecting the cuttings of Eucalyptus globulus, any of pot seedlings and open-planted seedlings may be used, and those of natural tree shape are preferred rather than those pruned to grow sprout branches . This is because, when hatching treatment, it is necessary to measure the growth of tree height and elongation of buds as an index of the hatching effect, and when measuring the elongation of the latter, the number of measurements in the pruned mother tree This is because there are many and complicated.

(Experimental example 1)
Among the Eucalyptus globulus clone seedlings, Sumarect (registered trademark) granules (0.6% active ingredient) were added to the parent tree clone A having a tree height of 100 cm to 110 cm, the mother tree clone B having a tree height of 100 cm to 120 cm, and the mother tree clone C having a tree height of 100 cm to 120 cm. ; Manufactured by Ishihara Sangyo Co., Ltd.) was dispersed and sprayed on the surface of the cultured soil so that the amount of the active ingredient was 1.25 mg and 6.25 mg in 1 L of the cultured soil. Cuttings were prepared at 4 weeks after soil application, and cuttings with a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Using the three strains in one of the experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 1 shows the results of tree height growth and rooting rate after 4 weeks of soil application.

(Experimental example 2)
Sumalect (registered trademark) granule (active ingredient 0.6%; manufactured by Ishihara Sangyo) is cultured using the cutting tree harvesting parent tree clone A with a tree height of 90 cm to 140 cm among Eucalyptus globulus clone pot seedlings grown on cuttings In 1 L of soil, the active ingredient was dispersed and sprayed on the surface of the pot culture soil so that the amount of the active ingredient was 6.25 mg. At the time of 2 weeks, 4 weeks, 8 weeks, and 12 weeks after the treatment, cuttings were prepared, and cuttings having a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Using the three strains in one of the experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 2 shows the results of tree height growth and rooting rate in each treatment period.

At the time of 1 month after the treatment, cuttings with a length of about 3 cm including two leaves were prepared. After cutting the cutting base with a knife and immersing it in a disinfectant solution for 5 seconds, apply 8000 ppm of indole butyric acid (IBA) / talc powder, insert it into the molding medium, and peat / vermiculite = 1: 1 (v / v) In the filled tray, this molding medium was put into a cutting seedling. Using the three strains in one of the experimental group. It was allowed to stand in a greenhouse at a temperature of 26 ° C. and a humidity of 80%, and after 6 weeks, the rooting rate was confirmed. Table 3 shows the results of tree height growth and rooting rate during one month after treatment.

Claims (4)

  In the method of growing Eucalyptus globulus cuttings, the plant growth regulator containing paclobutrazol as an active ingredient should be applied to the soil in an amount of 1.25 mg or more of the active ingredient per liter of cultivation soil of the cuttings The seedling method of the eucalyptus / globula cuttings mother tree which is the feature.   The seedling raising method of the Eucalyptus globulus cuttings heading mother tree of Claim 1 which makes the seedling raising period of the cutting heading mother tree from after the soil spraying of a plant growth regulator to cutting cutting work within one month.   The Eucalyptus / Globulus cuttings harvesting of the eucalyptus globulas according to claim 1, wherein the growth period of the cuttings harvesting mother tree from the time after the plant application of the plant growth regulator to the cuttings is set to a period in which the growth of the cuttings harvesting mother tree is suppressed within 20 cm. Mother tree raising method.   The method for raising seedlings of a eucalyptus / globula cuttings harvesting mother tree according to any one of claims 1 to 3, wherein the height of the cuttings harvesting mother tree is 90 to 140 cm.