JP2012029638A - Beer-tasting beverage with apple like and grapefruit like fruity flavor and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a beer-tasting beverage which has a fruity flavor tasting like an apple and a grapefruit and to provide a method for producing the same.SOLUTION: The beer-flavored beverage includes at least: (Z)-3-hexen-1-ol; linalool; β-citronellol; nerol; isobutyl isobutyrate; and cis-linalool oxide.

Description

  The present invention relates to a beer-taste beverage to which a fruit-like aroma accompanied by an apple-like or grapefruit-like scent is imparted and a method for producing the same.

  It is known that the scent imparted to beverages has a great influence on the character formation of beverage products. For example, hops are known to impart a refreshing bitterness and aroma to beer, and the aroma derived from hops has a great influence on the character formation of beer-taste beverages such as beer and sparkling liquor. In recent years, attention has been paid to the unique “Fukumika” felt when drinking beer and the aroma after drinking, and research has been conducted on the use of hops and their effects on flavor.

  The hop aroma is composed of hop essential oil contained in hop lupulin grains. When a specific material example is given about hop essential oil, linalool is a typical terpene compound contained in hop essential oil, and is a substance which gives a floral feeling. In addition, about 300 or more kinds of compounds constituting essential oils such as myrcene and α-, β-pinene as monoterpenes, α-humulene and β-caryophyllene as sesquiterpenes have been identified to date.

  Thus, although existence of various aroma components derived from hops is known, studies on aroma and chemical components in beverages have been limited (see Non-Patent Documents 1 to 5). That is, the correspondence between specific fragrances and chemical components is not clear, and particularly chemical components related to fragmented fragrance characteristics are hardly known.

T. Kishimoto et al., J. Agric. Food Chem., 54, 8855-8861, 2006 G. T. Eyres et al., J. Agric. Food Chem., 55, 6252-6261, 2007 V. E. Peacock, et al., J. Agric. Food Chem., 28, 774-777, 1980 K. C. Lam et al., J. Agric. Food Chem, 34, 763-770, 1986 V. E. Peacock et al., J. Agric. Food Chem., 29, 1265-1269, 1981

  An object of this invention is to provide the beer taste drink in which the fruit-like aroma accompanied by apple-like and grapefruit-like fragrance is felt, and its manufacturing method.

  By controlling the content of a specific component in a beverage within a predetermined range, the present inventors feel a fruit-like scent with a fruit-like scent such as an apple-like or grapefruit-like scent. It was found that a beer-taste beverage can be produced.

  That is, according to the present invention, the following inventions are provided.

(1) (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, β-citronellol, nerol, isobutylisobuty A beer-taste beverage comprising at least isobutyl isobutyrate and cis-linalool oxide,
The concentration range of each component is (Z) -3-hexen-1-ol: 1.2 to 2.6 ppb
・ Linalool: 16.2-469.2ppb
Β-citronellol: 5.0 to 15.3 ppb
・ Nellore: 3.6-14.8 ppb
Isobutyl isobutyrate: 1.7-34.3 ppb
Cis-linalool oxide: 5.7 to 29.5 ppb
And the concentration ratio of (Z) -3-hexen-1-ol, β-citronellol, nerol, isobutylisobutyrate, and cis-linalool oxide to linalool is (Z) -3-hexen-1-ol. : 0.0055 to 0.0735
Β-citronellol: 0.0325 to 0.3099
・ Nellore: 0.0316-0.2241
Isobutyl isobutyrate: 0.0454 to 0.1043
Cis-linalool oxide: 0.0539 to 0.3543
A range of beer-taste beverages.

(2) The beer-taste beverage according to (1), which is a malt beverage.

(3) The beer-taste beverage according to (1) or (2), which is a fermented beverage.

(4) The method for producing a beer-taste beverage according to any one of (1) to (3), comprising fermenting a pre-fermentation solution to which at least hops have been added.

(5) Hops are 2-nonanol
2-nonanone, and 2-pentanoic acid, 4-methyl-
Comprising
・ Γ-terpinene
Α-muurolene and α-terpinene
Comprising at least one of 2-tridecanone and 2-tridecanone
・ Butanoic acid, 2-methyl, 2-methylbutyl ester, and linalool
The production method according to (4), comprising at least one of the above.

  ADVANTAGE OF THE INVENTION According to this invention, the beer taste drink in which a fruit-like fragrance is felt, especially the beer taste drink in which the fruit-like fragrance accompanied by apple-like and grapefruit-like fragrance is provided. As shown in the examples below, beer-taste beverages that have a fruit-like scent with an apple-like, grapefruit-like scent have not been known so far and are not found in commercial products. The invention is advantageous in that it can provide a new type of beverage required by consumers. The beverage according to the present invention is also emphasized by a fruit-like scent with an apple-like or grapefruit-like scent derived from hops, and has a new scent that harmonizes with the sweet ester scent of a beer-taste beverage However, the present invention is also advantageous in that it can provide a beer-taste beverage with a harmonious aroma.

It is the figure which showed the result of having analyzed the relationship between the test brewery product test sections C, D, E, and F of Table 2, and the commercial products 1-18 of Table 5 by the principal component analysis.

Detailed description of the invention

Beer-taste beverage The beverage according to the present invention contains six aroma components, namely (Z) -3-hexen-1-ol, linalool, β-citronellol, nerol, isobutylisobutyrate, and cis-linalool oxide. It contains by content. In the beverage according to the present invention, the content of these six kinds of fragrance components can be set to the following ranges so that a fruit-like fragrance accompanied by an apple-like or grapefruit-like fragrance can be clearly felt.

(Z) -3-hexen-1-ol: 1.2-2.6 ppb
・ Linalool: 16.2-469.2ppb
Β-citronellol: 5.0 to 15.3 ppb
・ Nellore: 3.6-14.8 ppb
Isobutyl isobutyrate: 1.7-34.3 ppb
Cis-linalool oxide: 5.7 to 29.5 ppb

  The content of the aroma component in the beverage according to the present invention can be measured by gas chromatography with a mass spectrometer (GC / MS).

  In order to make the fruit-like aroma with apple-like and grapefruit-like aromas more clearly felt in the beverage according to the present invention, the concentration ratio of the aroma component to linalool can be set in the following range.

(Z) -3-hexen-1-ol: 0.0055 to 0.0735
Β-citronellol: 0.0325 to 0.3099
・ Nellore: 0.0316-0.2241
Isobutyl isobutyrate: 0.0454 to 0.1043
Cis-linalool oxide: 0.0539 to 0.3543

  Further, in order to make the fruit-like aroma with apple-like and grapefruit-like aromas more clearly felt in the beverage according to the present invention, the contents of the above-mentioned six kinds of aroma components are set as described above. And the density | concentration ratio of the aromatic component with respect to a linalool can be made into the above ranges.

  In the present invention, “beer-taste beverage” refers to a beverage having a taste and aroma peculiar to beer obtained when beer is usually produced, that is, when beer is produced based on fermentation by yeast or the like. Examples include fermented malt beverages such as beer, happoshu, and liqueurs, and non-fermented malt beverages such as completely alcohol-free malt beverages.

  The beverage according to the present invention can be provided in the form of a fermented beverage. In the present invention, “fermented beverage” means a beverage fermented with yeast.

  The beverage according to the present invention can be provided in the form of an alcohol-containing beverage containing alcohol. In the present invention, the “alcohol-containing beverage” is used to include a fermented beverage obtained by fermentation with yeast and a beverage to which alcohol is added.

  The beverage according to the present invention can be provided in the form of a malt beverage. In the present invention, the “malt beverage” means a beverage mainly composed of wheat and / or wort obtained from malt, and includes a malt soft drink imparted with a refreshing feeling by carbon dioxide gas or the like. Examples of the malt beverage include non-fermented malt beverages such as a completely alcohol-free malt beverage having an alcohol content of 0% by weight, and alcohol-containing malt beverages containing alcohol. Examples of the alcohol-containing malt beverage include a fermented malt beverage obtained by fermentation and a malt beverage to which alcohol has been added. Examples of malt beverages include non-alcohol fermented malt beverages obtained by removing alcohol, other low-boiling components and low-molecular components from fermented malt beverages obtained by fermentation.

  In the present invention, the “fermented malt beverage” means a beverage fermented by yeast using a carbon source, a nitrogen source, water and the like as raw materials, and using at least malt as a raw material. Examples of such fermented malt beverages include beer, sparkling liquor, liqueur (for example, beverages classified as “liqueur (foaming) (1)” under the liquor tax law) and the like. The beverage according to the present invention can be preferably provided in the form of a fermented malt beverage using at least water, hops, and malt as raw materials. In addition, the drink obtained by mixing fermented drinks, such as fermented malt drink, and a non-fermented drink is also contained in the drink by this invention.

  The beverage according to the present invention is not particularly limited to a malt beverage as long as it is a beer-taste beverage, and can also be provided in the form of a non-malt beverage that does not use wheat or malt. In the present invention, the “non-wheat beverage” includes a soft drink imparted with a refreshing feeling by carbon dioxide gas or the like. Non-wheat beverages include non-fermented beverages such as completely alcohol-free beverages having an alcohol content of 0% by weight, and alcohol-containing beverages containing alcohol. Examples of the alcohol-containing non-wheat beverage include fermented beverages obtained by fermentation and beverages to which alcohol is added. Examples of non-wheat beverages include non-alcoholic fermented beverages obtained by removing alcohol, other low-boiling components and low-molecular components from fermented beverages obtained by fermentation.

  According to one preferable aspect of the present invention, the beer-taste beverage is provided with a fruit-like aroma, and in a more preferable aspect, a fruit-like aroma with an apple-like or grapefruit-like aroma is provided. It will be.

Manufacture of beverages The beverage according to the present invention comprises six fragrance components, namely (Z) -3-hexen-1-ol, linalool, β-citronellol, nerol, isobutylisobutyrate, and cis-linalool oxide. It can manufacture by adding so that it may become content. For example, a beverage obtained without undergoing a fermentation process with yeast can be made into a beverage according to the present invention by adding the above-mentioned six kinds of aroma components.

  The acquisition source of these aromatic components is not particularly limited, and any commercially available product, one obtained by synthesis, or one isolated and purified from a natural product may be used.

  The beverage according to the present invention can also be produced by fermenting a pre-fermentation solution to which at least hops are added.

  When the beverage according to the present invention is a fermented malt beverage, it can be produced by fermenting a pre-fermentation solution comprising at least water, malt, and hops. In other words, fermented beer yeast is added to wort (pre-fermentation liquid) prepared from brewing raw materials such as malt and hops, followed by fermentation. By removing, the fermented malt beverage according to the present invention can be produced.

  In the above production procedure, wort can be produced according to a conventional method. For example, wort can be prepared by saccharifying a mixture of brewing raw materials and water, filtering to obtain wort, boiling the wort, and cooling the boiled wort. Hops can be added after boiling the wort or while boiling the wort.

  According to one preferable aspect of the present invention, in the production of the beverage of the present invention, hops can be added before boiling the pre-fermentation solution or after boiling the pre-fermentation solution.

As hops used in the production of the beverage according to the present invention,
・ 2-nonanol
Comprising 2-nonanone, and 2-pentanoic acid, 4-methyl-
・ Γ-Terpinene
Α-murolene, and at least one of α-terpinene, and 2-tridecanone butanoic acid, 2-methyl, 2-methylbutyl ester, and at least one of linalool Can be mentioned. Such hops include, but are not limited to, Taurus hops (country of origin: Germany).

  In order to make the fruit-like aroma with apple-like and grapefruit-like aromas more clearly felt in the beverage according to the present invention, the hop addition conditions can be set as follows, for example.

・ Add about 2 g / L of hops and boil the wort for about 10 minutes.

-Add 2g / L or more of hops after boiling the wort. Preferably, the wort is then allowed to stand at 80 to 95 ° C. for 20 to 60 minutes, assuming a general whirlpool standing.

  After boiling, by adding hops so as to be 5 g / L or less at the time of standing, the strength of the hop aroma did not become excessive, and a tendency to improve the degree of preference, that is, the preference was seen.

  In the method for producing a fermented malt beverage according to the present invention, in addition to hops and malt, auxiliary materials specified by liquor tax law such as rice, corn, corn, potato, starch, saccharides (for example, liquid sugar), protein degradation products and yeast Nitrogen sources such as extracts, fragrances, pigments, foaming / foaming improvers, water quality modifiers, fermentation aids and other additives can be used as brewing materials. Further, ungerminated barley (for example, ungerminated barley (including an extracted one), ungerminated wheat (including an extracted one)) may be used as a brewing raw material. The obtained fermented malt beverage should be (i) distilled at reduced pressure or atmospheric pressure to remove alcohol and low-boiling components, or (ii) removed alcohol and low-molecular components with a reverse osmosis (RO) membrane. Can also be used as a non-alcohol fermented malt beverage.

  When the beverage according to the present invention is a beer-taste fermented beverage that does not use wheat or malt, it is produced by fermenting a pre-fermentation solution comprising at least water and hops according to the production procedure of the fermented malt beverage. Can do. In addition to water and hops, carbon sources (for example, sugars such as liquid sugar) and nitrogen sources (for example, amino acid sources such as protein degradation products and yeast extract) can be added to the pre-fermentation solution. Accordingly, other additives such as fragrances, pigments, foaming / foaming improvers, water quality modifiers, fermentation aids, and the like can be added. The obtained beer-taste fermented beverage is (i) distilled under reduced pressure or atmospheric pressure to remove alcohol and low-boiling components, or (ii) removed alcohol and low-molecular components using a reverse osmosis (RO) membrane. By this, it can also be set as a non-alcohol beer taste fermented drink.

  According to another aspect of the invention, six fragrance components are included: (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, β- By adjusting citronellol (β-citronellol), nerol (nerol), isobutyl isobutyrate, and cis-linalool oxide to a predetermined content in a beer-taste beverage A method is provided for adjusting a beverage to a fruit-like aroma with an apple-like, grapefruit-like scent. Adjustment of content in the drink of an aromatic component may add these aromatic components, and may adjust it by selecting the kind of hop used as a raw material.

Moreover, the drink of this invention can also be manufactured as follows, for example.
Test brewing of fermented beverages using hops and analyzing aroma components in advance. From the result, a multiple regression equation with the fragrance component when the addition timing and addition amount of hops are used as variables is obtained. It can be produced by fermenting the pre-fermentation solution produced at the hop addition amount and the addition time, which are calculated using the multiple regression equation and each fragrance component falls within the desired range. At this time, the hop is
・ 2-nonanol
2-nonanone, and 2-pentanoic acid, 4-methyl-
Comprising
・ Γ-Terpinene
-Containing at least one of α-mulolene, and α-terpinene, and-2-tridecanone, butanoic acid, 2-methyl, 2-methylbutyl ester, and-containing at least one of linalool. It is preferable to use it. More preferably, the hop is used alone.

  The present invention will be specifically described based on the following examples, but the present invention is not limited to these examples.

Test Example 1: Aroma confirmation test and search for aroma components An aroma test after brewing was conducted on 63 hops of hops. The aroma of hops from Taurus (country of origin: Germany) was apple-like and grapefruit-like. It was found to be a fruit-like fragrance accompanied by the scent of fragrance (hereinafter, sometimes referred to as “the fragrance targeted by the present invention”).

  Next, the fragrance component was further searched for the 63 varieties of hops subjected to the fragrance test, and fragrance components having different concentrations between the hop varieties were searched. In the search, gas chromatography with a mass spectrometer (GC / MS) was used. Specifically, the fragrance component was extracted from the test sample with a C18 solid phase column and subjected to GC / MS. The internal standard method was used for quantification. Borneol was used as an internal standard substance and added to 25 ppb in the sample. The analysis conditions of the hop aroma component in GC / MS were as follows.

[GC / MS analysis conditions]
Capillary column: Trade name: HP-INNOWAX (length 60 m, inner diameter 0.25 mm, film thickness 0.25 μm)
Oven temperature: 40 ℃, 0.3min-3 ℃ / min → 240 ℃, 20min
Carrier gas: He, 10psi Low-pressure air transfer line Temperature: 240 ° C
MS ion source temperature: 230 ° C
MSQ pole temperature: 150 ° C
Front inlet temperature: 200 ℃
Monitoring ion: Same as quantitative ion
Ions used for quantification
Borneol m / z = 110
(Z) -3-hexen-1-ol m / z = 82
linalool m / z = 93
β-Citronellol m / z = 69
Nerol m / z = 93
phenethyl-2-methyl-butyrate m / z = 104
2-methoxy-4-vinylphenol m / z = 150
Isobutyl isobutyrate m / z = 89
Octanoic acid, ethyl ester m / z = 127
Cis-Linalool Oxide m / z = 94
ethyl 9-decenoate m / z = 127

As a result of the search, it was found that several tens of components correspond to components having different concentrations between varieties. Some of them were as described in Table 1 below.

  Multivariate statistical analysis such as cluster analysis was performed using multivariate analysis software “STATISTICA” for the fragrance with the characteristics of fruit-like aroma with apple-like and grapefruit-like scents. While confirming the correlation between the components (data omitted), confirm the aroma characteristics of each aroma component, and finally the following 10 types of aroma components are likely to be suitable as the target aroma index of the present invention. It was judged.

-Fruit-like aroma with floral-like: (Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), β-citronellol (β-citronellol), nerol (nerol), isobutyl iso Isobutyl isobutyrate, octanoic acid, ethyl ester, cis-linalool oxide, ethyl 9-decenoate
・ Fruit-like aroma with floral-like aroma: phenethyl-2-methyl-butyrate
・ Floral fragrance: linalool
・ Sweet and spicy flavor: 2-methoxy-4-vinylphenol

Example 1: Brewing Example (a) Brewing Method The storage sample used for evaluation was prepared using a 1.5 L scale apparatus. Charged wort adjusted to 13.5 degrees was used for the brewing test (67% malt use ratio, 33% use of secondary ingredients (rice, corn grits, corn starch)). In the brewing test, the prepared wort is boiled, and a predetermined amount of Taurus seed hop (source: Hopsteiner) is added during boiling or after standing after boiling (test areas A, B, C, D, E in Table 2 below). F). Specifically, the prepared wort was heated and boiled with an electric heater while adjusting the boiling strength to be constant and the evaporation rate to be 10% in 60 minutes. After boiling, the same amount of water as the amount of evaporation was added to the sample, and the wort was allowed to stand at 95 ° C. for 60 minutes. After filtering the filter paper, brewer's yeast was added to the wort that had been cooled with ice water, and the sample subjected to main fermentation for 1 week and post-fermentation for 4 days was used as a tasting sake sample.

  In test sections E and F, hops were added after boiling, and then allowed to stand at 95 ° C. for 1 hour.

(B) Component analysis and sensory evaluation The analysis of the aroma component of the obtained sake sample was performed by gas chromatography with a mass spectrometer (GC / MS) according to Test Example 1.

  Sensory evaluation of the obtained sake sample was performed by tasting with three skilled sensory evaluation panels. In sensory evaluation, the characteristics of the scent are expressed as the intensity of “fruit-like scent with apple-like and grapefruit-like scents”, 5: feel strongly and clearly, 3: feel, 1: feel weak, 0: I did not feel, scored.

The addition time and amount of hops, aroma component analysis values, and sensory evaluation results were as shown in Table 2 below.

  Regarding the sensory evaluation results, as shown in Table 2, the strongest 5 was obtained in the test sections E and F. In Test Zones C and D, 4 was obtained, and in Test Zone A, 3 was obtained. It was 1 in Test Zone B.

  In addition, the beverages in the test sections C, D, E, and F are emphasized with a fruit-like aroma accompanied by apple-like and grapefruit-like aromas derived from hops, and are in harmony with the sweet ester aroma of beer-taste beverages. It was confirmed that the beer beverage had a new fragrance.

(C) Multiple regression analysis Multiple regression analysis (multiple regression analysis) based on the concentration of each aroma component and hop addition conditions, that is, boiling time (minutes) after addition (hereinafter simply referred to as “boiling time”) and addition amount (g / L) Variable analysis software “STATISTICA”: Statsoft). In addition, since the test sections E and F were added after boiling, the “boiling time” was set to “0.1” and statistical analysis was performed. The results were as shown in Table 3.

Therefore, the multiple regression equation shown in the table is represented by the following equation for each component.
Predicted value of component = << coefficient (hour) x (boiling time (minute)) >> + << coefficient (amount) x (added amount (g / L)) >> + intercept Specific examples are as follows.
(Z) -3-Hexen-1-ol = <<-0.0114 × (boiling time (minutes)) >> + << 0.3456 × (addition amount (g / L)) >> + 0.7960
Linalool = <<-12.1170 x (boiling time (minutes)) + +32.3044 x (added amount (g / L)) >> 228.0476

  The multiple correlation coefficient showed a high value of 0.89 or more for many components, but phenethyl-2-methyl-butyrate, ethyl octoate, and ethyl 9-decanoate showed a low value of 0.6 or less. It was. Based on the coefficient of the multiple regression equation, the relation of each component with the aroma and the effectiveness as an index were examined. Here, if the target component is a volatile aroma component, the longer the boiling time of the hop, the smaller the component value, so the coefficient (time) is negative, the component value increases as the addition amount increases As the number increases, the coefficient (amount) should be positive.

  According to Table 3, for phenethyl-2-methyl-butyrate, 2-methoxy-4-vinylphenol, octanoic acid ethyl ester, and ethyl 9-decanoate, the coefficient (time) is positive (ie, the more it boils) And contradicts the actual event. Therefore, the concentration of phenethyl-2-methyl-butyrate, 2-methoxy-4-vinylphenol, ethyl octoate, and ethyl 9-decanoate is independent of hops or is determined by other factors. It was thought that it would not be an indicator of fragrance. From the above, the component for obtaining the target fragrance of the present invention is composed of (Z) -3-hexen-1-ol, linalool, β-citronellol, nerol, isobutylisobutyrate, and cis-linalool oxide. It was judged.

  Further, from the results of Tables 2 and 3, it is considered that the desirable ranges of the respective aroma component values are as follows.

(Z) -3-hexen-1-ol: 1.2-2.6 ppb
・ Linalool: 16.2-469.2ppb
Β-citronellol: 5.0 to 15.3 ppb
・ Nellore: 3.6-14.8 ppb
Isobutyl isobutyrate: 1.7-34.3 ppb
Cis-linalool oxide: 5.7 to 29.5 ppb

(D) Control of aroma component using multiple regression equation Using the regression equation obtained in the multiple regression analysis performed in (c) above, what conditions are set so that each aroma component value falls within a desirable range A simulation was performed to see if As a result, in the case of addition with a boiling time of 10 minutes, it was found that a desired range was obtained with a hop addition of 2.0 to 5.5 g / L. More specifically, in the case of addition with a boiling time of 10 minutes, (Z) -3-hexen-1-ol is 1.4 ppb, linalool is 171.5 ppb, even with the addition of 2.0 g / L of hops. It was found that β-citronellol was 8.7 ppb, nerol was 7.5 ppb, isobutyl isobutyrate was 11.1 ppb, and cis-linalool oxide was 11.1 ppb, which was within the desired range for each component.

(E) Composition ratio of fragrance component The composition ratio of each component was obtained from Table 2 based on linalool that is commonly present in hops and related to floral or hoppy fragrance. The results were as shown in Table 4.

  From Table 4, the range of the composition ratio of each fragrance component based on linalool is considered as follows.

(Z) -3-hexen-1-ol: 0.0055 to 0.0735
Β-citronellol: 0.0325 to 0.3099
・ Nellore: 0.0316-0.2241
Isobutyl isobutyrate: 0.0454 to 0.1043
Cis-linalool oxide: 0.0539 to 0.3543

Test Example 2: Comparison with commercial products The aromatic components of 18 types of commercial products (beer, happoshu, miscellaneous sake, liqueur) were analyzed. Specifically, analysis of the aroma component of a commercial product was performed by gas chromatography with a mass spectrometer (GC / MS) according to Test Example 2. The results were as shown in Table 5.

  Although the concentration of the fragrance component of the commercial product is within the range of the desired fragrance component concentration specified in Example 1 (c), that is, within the range of the beer-taste beverage according to the present invention, the value is shown in bold and underlined, There was no commercial product with all fragrance components within the range at the same time. That is, the case where all the fragrance components are within the range of the beer-taste beverage according to the present invention is the beer-taste beverage having the fragrance targeted by the present invention, but any commercially available product has at least one fragrance component according to the present invention. It was outside the concentration range of beer-taste beverages. In addition, as a result of sensory evaluation, there was no product that clearly felt a fruit-like aroma with an apple-like or grapefruit-like scent (data not shown). Therefore, it was confirmed from the component analysis and sensory evaluation test that there is no commercially available product having the fragrance targeted by the present invention. Moreover, although the fragrance characteristic of the commercial item 3 had a strong floral fragrance and a sweet fruit-like, it was different in character from the fruit-like fragrance accompanied by apple-like and grapefruit-like fragrances. In addition, although the commercial item 8 had a fruit-like fragrance, a fruit-like fragrance accompanied by an apple-like or grapefruit-like fragrance was not felt.

Next, the composition ratio based on linalool was calculated based on the analysis values in Table 5. The results were as shown in Table 6.

  A value in which the composition ratio of the fragrance component of the commercial product is within the range of the desirable composition ratio specified in Example 1 (e), that is, within the range of the composition ratio of each fragrance component of the beer-taste beverage according to the present invention is bold. As shown by the underline, there was no commercial product in which all fragrance components were within the range at the same time. Therefore, it was confirmed from the composition ratio of the component analysis values that there is no commercially available product having the fragrance targeted by the present invention.

Furthermore, in order to chart the relationship between the test brewery product test sections C, D, E and F in Table 2 and the commercial products 1 to 18 in Table 5, each component value was analyzed by principal component analysis (multivariate analysis software). "STATISTICA": Statsoft Inc.) Table 7 shows the eigenvectors, eigenvalues, contribution rates, and cumulative contribution rates of the principal component analysis results. The contribution ratio was 81% for factor 1, 3% for factor 3, and the cumulative contribution ratio for factors 1 and 3 reached 84%. FIG. 1 shows a scatter diagram of factor 1 and factor 3. The test sections C, D, E, and F, which had good aromas, were clearly distinguishable from the commercial products. This indicates that none of the commercially available products have the same component characteristics. As a result of the sensory test, the commercially available products do not have the target fragrance. I was able to verify.

Test Example 3: Analysis of hop component The component analysis of Taurus seed hop used in Example 1 was performed. Specifically, the essential oil component of Taurus seed hops was fractionally extracted with aqueous solutions having different ethanol concentrations and subjected to test brewing to identify the fractions from which the aroma targeted by the present invention was obtained. The components contained in the fraction were analyzed in the GC / MS scan mode according to the procedure of Test Example 1. As a result, it was found that the fraction that produces the aroma targeted by the present invention among the components contained in Taurus seed hops is composed of the components shown in Table 8.
Table 8: Ingredients contained in the essential oil fraction for obtaining the target fragrance of the present invention, camphene
・ Propanoic acid, 2-methyl-, 2-methylpropyl ester
1,6-octadiene, 2,7-dimethyl-
・ (-)-Β-pinene ((-)-β-pinene)
・ Pentanoic acid, 4-methyl-, methyl ester
・ Butanoic acid, 2-methyl-, 2-methylpropyl ester
・ 1-butanol, 3-methyl, propanoate
・ Dl-limonene
・ Sabinene
Β-cis-ciomene (1,3,6-octatriene, 3,7-dimethyl, (Z)-) (β-cis-ocimene = 1,3,6-Octatriene, 3,7-dimethyl-, ( Z)-)
・ 1,3,6-octatriene, 3,7-dimethyl, (E)-(1,3,6-octatriene, 3,7-dimethyl-, (E)-)
・ Cis-ocimene
・ Hexanoic acid, 5-methyl-, methyl ester
・ Γ-terpinene
・ Butanoic acid, 2-methyl-, 2-methylbutyl ester
・ Heptanoic acid, methyl ester
・ Pentyl 3-methylbutanoate (pentyl isovalerate) (n-amyl isovalerate)
2-buten-1-ol, 3-methyl- (2-buten-1-ol, 3-methyl-)
・ Methyl 4-methylhexa-2-enoate
・ Methyl 6-methyl heptanoate
-4H-cyclopenta [c] furan, hexahydro-1,1-dimethyl-4-methylene- (4H-cyclopenta [c] furan, hexahydro-1,1-dimethyl-4-methylene-)
3,5-dimethyl-1-heptyn-3-ene isomer
・ 2-nonanone
・ Hexanoic acid, methyl ester
・ Octanoic acid, methyl ester
・ Α-terpinolene
Bicyclo [3.1.0] hex-2-ene, 4,4,6,6-tetramethyl- (bicyclo [3.1.0] hex-2-ene, 4,4,6,6-tetramethyl-)
・ Α-terpinene
・ Furan, 3- (4-methyl-3-pentenyl)-
・ Nonanoic acid, methyl ester
・ 2-decanone
・ Copaene
・ 2-nonanol
・ 4-nonenoic acid, methyl ester
・ Linaroll
・ Methyl 8-methyl-nonanoate
・ 2-undecanone
・ Propanoic acid, 2-methyl-
・ Caryophyllene
・ Decanoic acid, methyl ester
・ Methyl Z-4-decenoate
・ 4-decenoic acid, methyl ester
Bicyclo [3.1.1] hept-2-ene-2-carboxaldehyde, 6,6-dimethyl- (bicyclo [3.1.1] hept-2-ene-2-carboxaldehyde, 6,6-dimethyl-)
Bicyclo [4.4.0] dec-1-ene, 2-isopropyl-5-methyl-9-ethylene- (bicyclo [4.4.0] dec-1-ene, 2-isopropyl-5-methyl-9- methylene-)
・ Aromadendrene
・ Δ-cadinene
2,6-octadienoic acid, 3,7-dimethyl-, methyl ester
・ 2-dodecanone
・ 2-heptadecanol
・ Valenesen 1 (valencene 1)
・ Cadina-1,4-diene
・ Β-selinene
・ Α-selinene
Eudesma-4 (14), 11-diene (eudesma-4 (14), 11-diene)
・ 3-methylene-1,5,5-trimethylcyclohexene (3-methylene-1,5,5-trimethylcyclohexene)
・ Rulepidadiene B (1,10-Dimethyl-2-isopropenylbicyclo [4.4.0] deca-3,5-diene) (rulepidadiene B (1,10-Dimethyl-2-isopropenylbicyclo [4.4.0] deca- 3,5-diene))
・ Naphthalene, 1,2,3,5,6,8a-Hexahydro-4,7-dimethyl-1- (1-methylethyl)-, (1S-cis)-(naphthalene, 1,2,3,5, 6,8a-hexahydro-4,7-dimethyl-1- (1-methylethyl)-, (1S-cis)-)
Naphthalene, 1,2,4a, 5,6,8a-hexahydro-4,7-dimethyl-1- (1-methylethyl)-(naphthalene, 1,2,4a, 5,6,8a-hexahydro-4 , 7-dimethyl-1- (1-methylethyl)-)
・ Selina-3,7 (11) -diene
Bicyclo [2.2.1] heptane, 2,2,3-trimethyl- (bicyclo [2.2.1] heptane, 2,2,3-trimethyl-)
・ Α-muurolene
・ 2-tridecanone
・ Cis-5-dodecenoic acid, methyl ester
・ 2,5-dimethoxy-3-methylnaphthalene
・ Naphthalene, decahydro-
・ 2,6-octadien-1-ol, 3,7-dimethyl-, (E)-(2,6-0ctadien-1-ol, 3,7-dimethyl-, (E)-)
・ 1S, cis-calamenene (β-Ionone)
・ 2-pentanoic acid, 4-methyl-
・ (+)-(3aR, 7aR) -2-Phenyl-trans-3a, 4,5,6,7,7a-Hexahydrobenzimidazole ((+)-(3aR, 7aR) -2-phenyl-trans- 3a, 4,5,6,7,7a-hexahydrobenzimidazole)
・ Α-calacorene
・ 2-butyl-2-cyclopenten-1-one
・ 1,4-cyclononadiene
1-cyclohexene-1-methanol, 4- (1-methylethenyl)-(1-cyclohexene-1-methanol, 4- (1-methylethenyl)-)
Bicyclo [2.2.2] oct-2-ene, 1,2,3,6-tetramethyl- (bicyclo [2.2.2] oct-2-ene, 1,2,3,6-tetramethyl-)
・ Octanoic acid
1,5,5,8-tetramethyl-12-oxabicyclo [9.1.0] dodeca-3,7-diene (1,5,5,8-tetramethyl-12-oxabicyclo [9.1.0] dodeca -3,7-diene)
・ 8,11,14-eicosatrienoic acid, (Z, Z, Z)-(8,11,14-eicosatrienoic acid, (Z, Z, Z)-)
・ Benzeneethanol, 4- (1-methylethyl)-(benzenemethanol, 4- (1-methylethyl)-)

  Next, the component that produces the aroma targeted by the present invention contained in Taurus seed hops is replaced with other varieties having different aroma characteristics from the aroma targeted by the present invention, such as Willamette, Super Pride, Pride of When compared with components such as Ringwood species, Nelson Sobin species, Galaxy species, etc., it was found that the characteristic component groups included in Taurus species hops are as follows.

(1) Ingredients unique to Taurus (unusual for other varieties)
・ 2-nonanol
2-nonanone, and 2-pentanoic acid, 4-methyl-
(2) Ingredients common to a limited number of other varieties: γ-terpinene
Α-muurolene and α-terpinene
(3) Common ingredient derived from hops, also included in other varieties, 2-tridecanone (2-tridecanone)
・ Butanoic acid, 2-methyl-, 2-methylbutyl ester, and linalool

  From the above, in order to obtain the target fragrance of the present invention, all the components of (1) are necessary for the hop component, and one or more of the common components of (2) and 1 of the common components of (3) More than seeds were considered necessary.

Claims (5)

(Z) -3-hexen-1-ol ((Z) -3-hexen-1-ol), linalool, β-citronellol, nerol, isobutyl isobutyrate isobutyrate), and a beer-taste beverage comprising at least cis-linalool oxide,
The concentration range of each component is (Z) -3-hexen-1-ol: 1.2 to 2.6 ppb
・ Linalool: 16.2-469.2ppb
Β-citronellol: 5.0 to 15.3 ppb
・ Nellore: 3.6-14.8 ppb
Isobutyl isobutyrate: 1.7-34.3 ppb
Cis-linalool oxide: 5.7 to 29.5 ppb
And the concentration ratio of (Z) -3-hexen-1-ol, β-citronellol, nerol, isobutylisobutyrate, and cis-linalool oxide to linalool is (Z) -3-hexen-1-ol. : 0.0055 to 0.0735
Β-citronellol: 0.0325 to 0.3099
・ Nellore: 0.0316-0.2241
Isobutyl isobutyrate: 0.0454 to 0.1043
Cis-linalool oxide: 0.0539 to 0.3543
A range of beer-taste beverages.   The beer-taste drink of Claim 1 which is a malt drink.   The beer taste drink of Claim 1 or 2 which is a fermented drink.   The manufacturing method of the beer taste drink as described in any one of Claims 1-3 which comprises fermenting the pre-fermentation liquid to which the hop was added at least. Hops are 2-nonanol
2-nonanone, and 2-pentanoic acid, 4-methyl-
Comprising
・ Γ-terpinene
Α-muurolene and α-terpinene
Comprising at least one of 2-tridecanone and 2-tridecanone
・ Butanoic acid, 2-methyl, 2-methylbutyl ester, and linalool
The manufacturing method of Claim 4 which comprises at least 1 sort (s) of.