JP2011219421A - 抗ウイルス、抗アレルゲン、抗ガス剤及び担体への固定化方法 - Google Patents
抗ウイルス、抗アレルゲン、抗ガス剤及び担体への固定化方法 Download PDFInfo
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Abstract
【解決手段】白金ナノコロイド溶液と有機ゲルマニウム溶液の固定化及びこれらの不活活性を強める。この抗ウイルス、抗アレルゲン、抗ガス剤を固定化した担体は、感染前の空気中や水中に浮遊するウイルス粒子、毒性タンパク質、アレルゲン、ガスなどからヒトやその他の生物を守ることができる。この場合、白金ナノコロイド溶液と有機ゲルマニウム溶液とを同時に混合して用いることもできるが、担体に有機ゲルマニウム溶液を吸着固定後、白金ナノコロイド溶液で処理すれば、さらに相乗効果を高めることができる。
【選択図】図1
Description
また、この発明に係る抗ウイルス、抗アレルゲン、抗ガス剤の担体への吸着固定方法は、担体に有機ゲルマニウム溶液を吸着固定後、白金ナノコロイド溶液で処理するものである。この場合、前記担体としては、不職布、ガラス、ステンレス又は種々ポリマー等が適している。
蒸留水中5mMのH2PtCl6のコロイド粒子溶液を50ml、これに5mMクエン酸溶液50mlを混合後、8時間静置する。その後、100mMクエン酸溶液を2.5ml混合して、1週間静置後、白金ナノコロイド液として用いた。
通常、5gの有機ゲルマニウムナノ粒子を約1リッターの蒸留水に加え、撹拌し溶解させて、0.5%ゲルマニウム原液を調整し、適宜、希釈して用いた。
白金ナノコロイド溶液と有機ゲルマニウム溶液とを1:1で混合した後、25mMトリスーHCl(pH8.0)等の緩衝液をこの混合液の1/2量程加え、pH4.0付近の弱酸性に調整した。
評価(1)
特定の細菌(ワサビ軟腐病菌(Pectobacterium carotovorumグループ)を宿主として、これに特異的に感染し、溶菌させるファージEr2,大腸菌に特異的に感染するλファージの2種類を用いた。ここでは、後者を用いた結果のみ載せているが、前者のファージを用いた場合も、全く同様な結果を得ている。
不織布を、白金ナノコロイド混合液の5倍希釈液に1時間浸漬し、乾燥後、1mlあたり10億個の細菌ウイルス(λファージ)を含む液を100μlをスポットし、SMバッファー100μl加えて、再懸濁し、この10μlを大腸菌を上層に含む重層培地(軟寒天上層培地)に置床した。37℃で培養すると、白金ナノコロイド混合液で処理しなかったコントロール(図1、左側)では溶菌反応が見られたが、処理した区(図1、右側)では、溶菌反応が全く見られなかった。即ち、白金ナノコロイド混合液によって細菌ウイルスが不活性化されたことが分かった。
不織布を、0.1パーセント有機ゲルマニウム液に1時間浸漬し、乾燥後、何も加えない(Geのみ)、Agナノ液10倍希釈液で浸漬した(Ge+Ag)、Pt液10倍希釈液で浸漬した(Ge+Pt)後、再度、乾燥後、1mlあたり10億個の細菌ウイルス(λファージ)を含む液を50μlをスポットし、SMバッファー100μlを加えて、再懸濁し、この10μlを大腸菌を上層に含む重層プレート上(軟寒天上層プレート)に滴下した。無処理(図1、左端)、Geのみの区(図1、左から2番目)では溶菌反応が見られたが、Agで処理した区(図2、右側から2番目)、Ptで処理した区(図1、右端)では、溶菌反応が全く見られなかった。Ag,Pt液追加処理によって細菌ウイルスが不活性化されたことが分かった。
Claims (3)
- 白金ナノコロイド溶液と有機ゲルマニウムナノコロイド溶液との混合液を主成分とすることを特徴とする抗ウイルス、抗アレルゲン、抗ガス剤。
- 担体に有機ゲルマニウムナノコロイド溶液を吸着固定後、白金ナノコロイド溶液で処理することを特徴とする抗ウイルス、抗アレルゲン、抗ガス剤の担体への吸着固定方法。
- 請求項2の抗ウイルス、抗アレルゲン、抗ガス剤の担体への吸着固定方法において、前記担体が不職布、ガラス、ステンレス又は種々ポリマーであることを特徴とする抗ウイルス、抗アレルゲン、抗ガス剤の担体への吸着固定方法。
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JP2011501977A (ja) * | 2007-06-11 | 2011-01-20 | ナノポリ カンパニー リミテッド | 抗菌及び抗カビ機能を有する含水性ティッシュペーパの製造方法 |
JP2011156114A (ja) * | 2010-01-29 | 2011-08-18 | Luckys:Kk | 除菌消臭水の製造方法 |
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JP2011501977A (ja) * | 2007-06-11 | 2011-01-20 | ナノポリ カンパニー リミテッド | 抗菌及び抗カビ機能を有する含水性ティッシュペーパの製造方法 |
JP2011156114A (ja) * | 2010-01-29 | 2011-08-18 | Luckys:Kk | 除菌消臭水の製造方法 |
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JP2019113515A (ja) * | 2017-12-25 | 2019-07-11 | 株式会社 Eu−BS | 放射性物質吸着体製造方法と放射性物質吸着体と放射性物質回収方法 |
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