JP2011200854A - Dry sterilization method of crustacea waste, and dry sterilized powder of crustacea - Google Patents
Dry sterilization method of crustacea waste, and dry sterilized powder of crustacea Download PDFInfo
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- JP2011200854A JP2011200854A JP2010089815A JP2010089815A JP2011200854A JP 2011200854 A JP2011200854 A JP 2011200854A JP 2010089815 A JP2010089815 A JP 2010089815A JP 2010089815 A JP2010089815 A JP 2010089815A JP 2011200854 A JP2011200854 A JP 2011200854A
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- crustacean
- dry
- crustacea
- shrimp
- waste
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Abstract
Description
本発明は、廃棄甲殻類の乾燥殺菌処理方法に関する。詳しくは、廃棄甲殻類の有用成分の脂質、蛋白質、アミノ酸、炭水化物、食物繊維等の腐敗防止と凝固・硬質化を防止して、有用成分を抽出され易くする廃棄甲殻類の乾燥殺菌処理方法および甲殻類の乾燥殺菌粉末に関するものである。 The present invention relates to a method for dry sterilization treatment of waste crustaceans. Specifically, a method for drying and sterilizing waste crustaceans that makes it easy to extract useful components by preventing the decay, coagulation and hardening of useful components such as lipids, proteins, amino acids, carbohydrates, and dietary fibers of waste crustaceans It relates to crustacean dry sterilized powder.
甲殻類のエビ生産国はインドネシア、タイ、ベトナム、インド、マレーシア、中国等が挙げられる。原産地より出荷される際エビは不飽和脂肪酸が多く腐敗が激しいことから、頭部や外殻を除去する加工処理が行われる。その際、廃棄されるエビの頭部および外殻の総廃棄量は、現在、年間１５，０００トン以上の莫大な量が廃棄され、極微量である一部は生のまま鳥の餌として、また、外殻の一部はキチン・キトサン加工に使用されているが、大部分は海岸や野山に不法投棄処分され、腐敗が早いため悪臭発生源となり、環境悪化の源となっているのが現状である。
更に、これらエビ生産国では、エビの頭部や外殻を加工工場独自で廃棄処理する方法が常法となっていること、また、廃棄する場合には、運賃や人件費が必要のことから、各国のエビ加工工場に於いて野積みになり悪臭発生源となっている。Crustacean shrimp producing countries include Indonesia, Thailand, Vietnam, India, Malaysia and China. When shipped from the place of origin, shrimp are heavily unsaturated and rotted, so processing is done to remove the head and shell. At that time, the total amount of shrimp heads and shells to be discarded is currently discarded in an enormous amount of 15,000 tons or more per year. Some of the outer shells are used for chitin and chitosan processing, but most of them are dumped illegally on the coast and in the mountains, and because of the early decay, it is a source of odors and a source of environmental degradation. Currently.
In addition, in these shrimp producing countries, it is common practice to dispose of shrimp heads and shells on their own at the processing plant. In the shrimp processing factories in various countries, it has become a source of foul odors.
これまで、魚介類廃棄物の蛋白質を、蛋白質分解酵素とともに攪拌して、魚介類由来の蛋白質などを含む有用物質およびその製造方法が［特許文献１］に開示されている。
また、有機廃棄物を高温高圧蒸気１８０℃〜２２０℃、１０〜３０ｋｇ／ｃｍ２で処理する方法と得られる処理物が［特許文献２］に提案されている。So far, a useful substance containing a protein derived from seafood and the like and a method for producing the same have been disclosed in [Patent Document 1] by stirring the protein of a seafood waste together with a proteolytic enzyme.
[Patent Document 2] proposes a method of treating organic waste at a high temperature and high pressure steam of 180 ° C. to 220 ° C. and 10 to 30 kg / cm 2 and the obtained treated product.
［特許文献１］は、蛋白質分解酵素のエンド型、エキソ型、エンド・エキソ型、それぞれの組合せにより、魚介類有用成分の水溶性アミノ酸を含む分子量３０，０００以下のオリゴ蛋白質の他、分子量２０，０００〜１００，０００の脂肪酸を製造することであるが、かくの如く、蛋白質分解酵素のみによる魚介類の蛋白質の分解には、極めて長期間の分解時間が必要となるのみでなく、低分子のアミノ酸を得ることは困難である。また、［特許文献２］の提案は、有機廃棄物を直接高温乾燥するので、有用成分である蛋白質は硬質化およびアミノ酸の熱分解が起きることは常識であり、この乾燥物から有用成分を抽出するには、乾燥方法に重大な欠点がある。[Patent Document 1] describes a proteolytic enzyme endo-type, exo-type, endo-exo-type, each combination of oligoproteins having a molecular weight of 30,000 or less, including a water-soluble amino acid that is a useful component of seafood, and a molecular weight of 20 In this way, the degradation of fish and shellfish proteins using only proteolytic enzymes requires not only a very long degradation time but also low molecular weight. It is difficult to obtain the amino acid. In addition, the proposal of [Patent Document 2] directly dries organic waste at high temperature, so it is common knowledge that proteins that are useful components are hardened and pyrolysis of amino acids occurs, and useful components are extracted from this dried product. There are significant drawbacks to the drying method.
前記した従来法には、有機廃棄物を乾燥殺菌脱水すると有機成分組織が硬化・凝縮等の変形があり、有用成分の抽出性が低下する基本的な問題意識の欠如がある。
また、魚介類のアミノ酸抽出に蛋白質分解酵素が用いられているものの、低分子量のアミノ酸は得られていない。
即ち、原料である有機廃棄物や魚介類の初期処理（乾燥前処理）の欠如および蛋白分解酵素によるアミノ酸生成に限界があり、外的エネルギーを補充する必要があることから、低分子量アミノ酸を得ること等々に課題がある。In the conventional method described above, there is a lack of basic problem awareness that organic component tissue undergoes deformation such as hardening and condensation when organic waste is dried, sterilized and dehydrated, and extractability of useful components is reduced.
In addition, although proteolytic enzymes are used to extract amino acids from seafood, no low molecular weight amino acids have been obtained.
In other words, there is a lack of initial treatment (pre-drying treatment) of organic waste and seafood as raw materials, and there is a limit to amino acid production by proteolytic enzymes, and it is necessary to replenish external energy, thus obtaining low molecular weight amino acids. There are problems.
本発明は、廃棄甲殻類のエビの頭部および外殻に、天然ミネラルイオン含有パイナップル酵素果汁液を浸漬・含浸させ、乾燥することにより有用成分の軟質化を促進し、蛋白質の凝固・硬質化を抑制し低分子アミノ酸、ペプチド蛋白質、脂質、食物繊維、ナトリウム、リン、鉄分、カルシウム、マグネシウム、亜鉛、マンガン等を含有する乾燥殺菌粉末および液体抽出を容易にする技術を確立することが目的である。 The present invention promotes softening of useful components by immersing and impregnating pineapple enzyme juice containing natural mineral ions in the crust head and outer shell of waste crustaceans and drying, thereby solidifying and hardening the protein. For the purpose of establishing a technology that facilitates dry sterilization powder and liquid extraction containing low molecular weight amino acids, peptide proteins, lipids, dietary fiber, sodium, phosphorus, iron, calcium, magnesium, zinc, manganese, etc. is there.
本発明は、生産現地の廃棄甲殻類のエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸透・含浸させるとき、パイナップル酵素とエビの頭部および外殻の有用成分の複合蛋白質の基質と結合して、初期加水分解の状態となり、これを過熱蒸気乾燥する外的エネルギーを付与することにより、酵素触媒が促進され、乾燥甲殻類の硬質化・凝固が抑制され、低分子アミノ酸、ペプチド蛋白質、脂質、食物繊維、ナトリウム、リン、鉄分、カルシウム、マグネシウム、亜鉛、マンガン等を含有する乾燥殺菌粉末および高濃度の低分子アミノ酸類を容易に抽出することを見出し、本発明を完成した。 The present invention relates to a complex protein of pineapple enzyme and a useful component of shrimp head and shell when penetrating and impregnating pineapple enzyme juice containing natural mineral ions into shrimp head and shell of waste crustacea at the production site By binding to the substrate of the product, it becomes a state of initial hydrolysis, and by giving external energy to dry this by superheated steam, the enzyme catalyst is promoted, the hardening and coagulation of the dried crustacea is suppressed, and the low molecular amino acid , Found that it can easily extract dry sterilized powder and high-concentration low molecular weight amino acids containing peptide protein, lipid, dietary fiber, sodium, phosphorus, iron, calcium, magnesium, zinc, manganese, etc. did.
本発明は、生産現地のみならず廃棄甲殻類のエビの頭部は不飽和脂肪酸が多く含有されるため、外殻と共に腐敗防止について、これまで、食肉および魚類の切り身の鮮度保持（ドリップ防止）や酸化防止剤として使用したパイナップル酵素果汁液をエビの頭部および外殻に含浸し、天日乾燥したところ、乾燥エビに異臭がなくて硬質化は起こらなかった。この乾燥エビを酵素抽出したところ、低分子のアミノ酸が抽出成分中に多く含まれることがわかった。しかし、甲殻類は、東南アジアの海岸に近い池での養殖および自然界で養殖していることから、一般細菌、大腸菌類が付着している可能性があり、殺菌を兼ねて過熱蒸気乾燥殺菌処理し、脂質、蛋白質、アミノ酸、炭水化物等の抽出成分濃度を高めることを加えた。 In the present invention, not only the production site but also the crust head of discarded crustaceans contains a large amount of unsaturated fatty acids, so that the freshness of meat and fish fillets has been maintained (preventing drip) so far with anti-corrosion along with the outer shell. When the shrimp head and outer shell were impregnated with pineapple enzyme juice used as an antioxidant and sun-dried, the dried shrimp had no off-flavor and did not harden. When this dried shrimp was subjected to enzyme extraction, it was found that many low-molecular amino acids were contained in the extracted components. However, because crustaceans are cultivated in ponds close to the coast of Southeast Asia and naturally cultivated, general bacteria and coliforms may be attached. Added to increase the concentration of extracted components such as lipids, proteins, amino acids, carbohydrates.
即ち、本発明は、廃棄甲殻類のエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸透・含浸させて、パイナップル酵素の触媒作用を受ける蛋白質の基質と一旦酵素分子と結合させ、加水分解反応を起こしやすくした後、これを過熱蒸気乾燥殺菌する外的エネルギーを付与することにより、蛋白質を各種アミノ酸成分に分解し低分子化させ、加水分解反応が促進し柔らかく効率が良い状態で乾燥処理した甲殻類の粉末を得る。該乾燥処理甲殻類を微粉砕した後、常法の抽出方法であるアルコール６０％、水４０％および温水抽出、有機溶媒を用いての抽出が容易であり、抽出溶液中には低分子アミノ酸類のアルギニン（Ａｒｇ）、リジン（Ｌｙｓ）、ヒスチジン（Ｈｉｓ）、フェニルアラニン（Ｐｈｅ）、チロシン（Ｔｙｒ）、ロイシン（Ｌｅｕ）、イソロイシン（Ｉｌｅ）、メチオニン（Ｍｅｔ）、バリン（Ｖａｌ）、アラニン（Ａｒａ）、グリシン（Ｇｌｙ）、プロリン（Ｐｒｏ）、グルタミン（Ｇｌｎ）、グルタミン酸（Ｇｌｕ）、セリン（Ｓｉｒ）、トレオニン（Ｔｈｒ）、アスパラギン（Ａｓｎ）、アスパラギン酸（Ａｓｐ）、トリプトファン（Ｔｒｐ）、シスチン（Ｃｙｓ）を高濃度組成およびペプチド蛋白質、脂質、炭水化物、食物繊維、ナトリウム（Ｎａ）、リン（Ｐ）、鉄（Ｆｅ）、カルシウム（Ｃａ）、マグネシウム（Ｍｇ）、亜鉛（Ｚｎ）、マンガン（Ｍｎ）等を得ることを見出し、本発明を完成するに至った。 That is, the present invention is to impregnate and impregnate pineapple enzyme juice containing natural mineral ions into the crust head and outer shell of waste crustaceans, and once bind the enzyme substrate with the protein substrate catalyzed by the pineapple enzyme. , After making the hydrolysis reaction easy to occur, by applying external energy to sterilize it by superheated steam drying, the protein is decomposed into various amino acid components to reduce the molecular weight, and the hydrolysis reaction is promoted to be soft and efficient Crustacean powder dried with After finely pulverizing the dried crustacean, extraction with an ordinary method of alcohol 60%, water 40% and warm water, and extraction with an organic solvent is easy. Arginine (Arg), Lysine (Lys), Histidine (His), Phenylalanine (Phe), Tyrosine (Tyr), Leucine (Leu), Isoleucine (Ile), Methionine (Met), Valine (Val), Alanine (Ara) Glycine (Gly), proline (Pro), glutamine (Gln), glutamic acid (Glu), serine (Sir), threonine (Thr), asparagine (Asn), aspartic acid (Asp), tryptophan (Trp), cystine (Cys) ) High concentration composition and peptide protein, lipid, carbohydrate, dietary fiber, sodium Na), phosphorus (P), iron (Fe), calcium (Ca), magnesium (Mg), zinc (Zn), found that to obtain a manganese (Mn), or the like, and completed the present invention.
本発明のアミノ酸成分と濃度分析は（社）日本食品分析センター（第３０６０４０２６４号）および（第３０６０２０６５５号）、（第３０８０２０６７４号）、（Ｎｏ３０６０１０４３７）、インドネシア ボゴール大学（ＬＴ−４０５−５４１）、（有）エス・エヌ・イー総合研究所（第２００７０３２０）で行った。 Amino acid components and concentration analysis of the present invention were conducted by the Japan Food Analysis Center (No. 30604026) and (No. 306062065), (No. 30802664), (No. 306010437), Indonesia Bogor University (LT-405-541), ( Yes) conducted at SNR Research Institute (No. 200703320).
本発明は、以下の構成から成り立っている。
（１）廃棄甲殻類であるエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵 素果汁液を浸透・含浸させた後、過熱蒸気乾燥殺菌後、粉砕処理する廃棄甲殻類の 乾燥殺菌処理方法および甲殻類の乾燥殺菌粉末。
（２）天然ミネラルイオン含有パイナップル酵素果汁液が、パイナップル皮果汁液に天然 鉱石のヒル石、麦飯石、異王石、ゼオライトを単独ないし複数浸漬して得るカルシ ウム、ナトリウム、マグネシウム、カリウム、鉄、アルミニウム、亜鉛、ケイ素等 のミネラルイオンから成る前記（１）に記載する廃棄甲殻類の乾燥殺菌処理方法お よび甲殻類の乾燥殺菌粉末。
（３）廃棄甲殻類であるエビの頭部および外殻を乾燥殺菌する前に、天然ミネラルイオン 含有パイナップル酵素果汁液を含浸し、腐敗防止と悪臭発生防止すると共に有用成 分の軟化を促進する前記（１）、（２）に記載する廃棄甲殻類の乾燥殺菌処理方法 。
（４）甲殻類の乾燥殺菌粉末の有用成分が抽出成分中に、低分子アミノ酸のアルギニン（ Ａｒｇ）、リジン（Ｌｙｓ）、ヒスチジン（Ｈｉｓ）、フェニルアラニン（Ｐｈｅ ）、チロシン（Ｔｙｒ）、ロイシン（Ｌｅｕ）、イソロイシン（Ｉｌｅ）、メチオ ニン（Ｍｅｔ）、バリン（Ｖａｌ）、アラニン（Ａｒａ）、グリシン（Ｇｌｙ）、 プロリン（Ｐｒｏ）、グルタミン（Ｇｌｎ）、グルタミン酸（Ｇｌｕ）、セリン（ Ｓｉｒ）、トレオニン（Ｔｈｒ）、アスパラギン（Ａｓｎ）、アスパラギン酸（Ａ ｓｐ）、トリプトファン（Ｔｒｐ）、シスチン（Ｃｙｓ）を高濃度に抽出されやす くした前記（１）、（２）、（３）に記載の甲殻類の乾燥殺菌粉末。The present invention comprises the following configurations.
(1) A method for dry sterilization of waste crustaceans, which is made by impregnating and impregnating pineapple fermented fruit juice containing natural mineral ions into the head and outer shells of shrimp, a waste crust, followed by superheated steam drying and sterilization And crustacean dry pasteurized powder.
(2) Natural mineral ion-containing pineapple enzyme juice is obtained by immersing one or more natural ores of leeches, barleystone, heteroeolite, and zeolite in pineapple peel juice, calcium, sodium, magnesium, potassium, iron, The method for dry sterilization of discarded crustaceans and dry sterilized powder of crustaceans as described in (1) above, comprising mineral ions such as aluminum, zinc and silicon.
(3) Before drying and sterilizing shrimp heads and shells, which are discarded crustaceans, impregnate pineapple enzyme juice containing natural mineral ions to prevent spoilage and odors and to promote softening of useful components. The method for drying and sterilizing waste crustaceans described in (1) and (2) above.
(4) The useful components of the dry sterilized powder of crustaceans include low molecular weight amino acids arginine (Arg), lysine (Lys), histidine (His), phenylalanine (Phe), tyrosine (Tyr), leucine (Leu) ), Isoleucine (Ile), methionine (Met), valine (Val), alanine (Ara), glycine (Gly), proline (Pro), glutamine (Gln), glutamic acid (Glu), serine (Sir), threonine ( Thr), asparagine (Asn), aspartic acid (Asp), tryptophan (Trp), and cystine (Cys), which are easily extracted at high concentrations, the crustaceans according to (1), (2) and (3) above Dry sterilized powder.
本発明に於いて、甲殻類のエビを生産現地加工工場において、エビ本体から除かれた直後のエビの頭部および外殻は、腐敗防止のため天然ミネラルイオン含有パイナップル酵素果汁液を含浸処理される。これによりエビの不飽和脂肪酸による酸化を防止し、蛋白質群はパイナップル酵素の触媒作用を受け、加水分解が起こり易くできる。 In the present invention, the shrimp head and outer shell immediately after being removed from the shrimp body at a local processing plant that produces crustacean shrimp are impregnated with pineapple enzyme juice containing natural mineral ions to prevent spoilage. The This prevents the shrimp from being oxidized by unsaturated fatty acids, and the protein group is catalyzed by the pineapple enzyme and can be easily hydrolyzed.
従来、エビの生産現地では、エビの頭部は生の状態で一部は鳥の餌として与えるが、殆どは生の状態で海岸や野山に不法投棄されているのが通常になっている。また、外殻の一部はキチン・キトサンの加工に使用されているが、殆どは、頭部と同様に廃棄処分され、悪臭による環境悪化を招いている。
エビの生産現地では、廃棄物となるエビの頭部および外殻の取り扱いに対し、本発明の天然ミネラルイオン含有パイナップル酵素果汁液にエビの頭部および外殻を浸漬・含浸させ、エビの頭部に含まれる不飽和脂肪酸および蛋白質の酸化および腐敗を防止し、アミノ酸、脂質や蛋白質、炭水化物、食物繊維等の腐敗防止をすることは発明者による全く初の知見である。Traditionally, at shrimp production sites, shrimp heads are raw and some are fed as bird food, but most are raw and usually dumped illegally on the coast or in the mountains. A part of the outer shell is used for processing chitin / chitosan, but most of the outer shell is disposed of in the same manner as the head, resulting in environmental degradation due to bad odor.
In the shrimp production site, the shrimp head and outer shell are soaked and impregnated in the pineapple enzyme juice containing natural mineral ions of the present invention for handling the shrimp head and outer shell as waste. It is the first discovery by the inventor to prevent the oxidation and decay of unsaturated fatty acids and proteins contained in food parts, and to prevent the decay of amino acids, lipids and proteins, carbohydrates, dietary fibers and the like.
本発明のエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸透・含浸させた後、これを過熱蒸気乾燥殺菌処理した甲殻類の乾燥物の成分は硬質・固化することなく食品微粉砕機により極微細粉末を得ことができる。 The crustacean dried product obtained by impregnating and impregnating the pineapple enzyme fruit juice containing natural mineral ions into the shrimp head and outer shell of the present invention, and then subjecting it to superheated steam drying and sterilization is a hard and solidified ingredient. An ultrafine powder can be obtained by a pulverizer.
本発明者は、前記した甲殻類の乾燥物が硬質・固化しなかったことについて、甲殻類の蛋白質の加水分解反応が、湿式処理による加水分解に加え、耐高熱性のパイナップル酵素が含浸した蛋白質の加水分解反応を促進した結果、低分子化アミノ酸加水分解生成を可能にしたものと考えられる。 The present inventor confirmed that the crustacean protein was impregnated with a high-heat-resistant pineapple enzyme in addition to hydrolysis by wet processing, because the crustacean dried product was not hardened or solidified. As a result of promoting the hydrolysis reaction, it is considered that low molecular weight amino acid hydrolysis can be produced.
本発明は、廃棄甲殻類のエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸透・含浸した後、過熱蒸気乾燥殺菌処理した甲殻類の蛋白質から低分子のアミノ酸成分２０種、ペプチド蛋白質、脂質、炭水化物等の高濃度粉末および液体抽出を可能にしたことは、食品添加物、健康食品、化粧品、医薬品、飼料、ペットフード、肥料等の産業応用への効果は多大である。 The present invention comprises 20 kinds of low-molecular amino acid components from crustacean proteins that have been impregnated and impregnated with natural mineral ion-containing pineapple enzyme juice into the crust head and outer shell of discarded crustaceans, and then subjected to superheated steam drying and sterilization treatment. Enabling high-concentration powder and liquid extraction of peptide proteins, lipids, carbohydrates, and the like has a great effect on industrial applications such as food additives, health foods, cosmetics, pharmaceuticals, feeds, pet foods, and fertilizers.
以上の説明から成るように、本発明にあたっては、次に列挙する効果が得られる。
（１）（［請求項１］の構成要件）とで構成されるエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸透・含浸させることにより、エビの頭部および外殻の不飽和脂肪酸および蛋白質の腐敗防止と共に加水分解を促進し、乾燥殺菌粉末から低分子アミノ酸２０種およびペプチド蛋白質、脂質、炭水化物、各種ミネラル成分等多種類有用成分を高濃度で得られる。As described above, in the present invention, the following effects can be obtained.
(1) (Natural mineral ion-containing pineapple enzyme juice liquid is infiltrated and impregnated into the shrimp head and shell composed of It prevents hydrolysis of unsaturated fatty acids and proteins, promotes hydrolysis, and provides a high concentration of 20 kinds of low molecular amino acids and various useful ingredients such as peptide proteins, lipids, carbohydrates, and various mineral components from the dry sterilized powder.
（２）（［請求項２］の構成要件）とで構成されているので、前記（１）の加水分解酵素であり、乾燥殺菌粉末からの低分子アミノ酸成分の抽出性を容易にする効果が得られる。(2) (Constituent requirement of [Claim 2]), the hydrolase of (1), and the effect of facilitating the extraction of low molecular weight amino acid components from the dry sterilized powder can get.
（３）（［請求項３］の構成要件）も前記（２）と同様な効果が得られる。(3) (Constitutional requirement of [Claim 3]) can also achieve the same effect as the above (2).
（４）（［請求項４］の構成要件）とで構成されている前記（１）、（２）の乾燥殺菌粉末から、低分子化アミノ酸全てのアルギニン（Ａｒｇ）、リジン（Ｌｙｓ）、ヒスチジン（Ｈｉｓ）、フェニルアラニン（Ｐｈｅ）、チロシン（Ｔｙｒ）、ロイシン（Ｌｅｕ）、イソロイシン（Ｉｌｅ）、メチオニン（Ｍｅｔ）、バリン（Ｖａｌ）、アラニン（Ａｒａ）、グリシン（Ｇｌｙ）、プロリン（Ｐｒｏ）、グルタミン（Ｇｌｎ）、グルタミン酸（Ｇｌｕ）、セリン（Ｓｉｒ）、トレオニン（Ｔｈｒ）、アスパラギン（Ａｓｎ）、アスパラギン酸（Ａｓｐ）、トリプトファン（Ｔｒｐ）、シスチン（Ｃｙｓ）を高濃度で液体抽出し易くする効果が得られる。(4) From the dry sterilized powders of (1) and (2), which are composed of (constituent requirements of [Claim 4]), arginine (Arg), lysine (Lys), histidine of all low molecular weight amino acids (His), phenylalanine (Phe), tyrosine (Tyr), leucine (Leu), isoleucine (Ile), methionine (Met), valine (Val), alanine (Ara), glycine (Gly), proline (Pro), glutamine (Gln), glutamic acid (Glu), serine (Sir), threonine (Thr), asparagine (Asn), aspartic acid (Asp), tryptophan (Trp), and cystine (Cys) can be easily extracted at high concentrations. can get.
本発明は、上記した構成からなる廃棄甲殻類のエビの頭部および外殻の乾燥殺菌処理方法および甲殻類の乾燥殺菌粉末を実施する最も好ましい実施形態を詳しく説明する。
本発明は甲殻類であれば、イセエビ、ウチワエビ、クルマエビ、ブラックタイガー，大正エビ、バナメー、ホワイト、南米ピンク、ウシエビ、オキアミ、ズワイガニ、毛ガニ、ワタリガニ、上海ガニ、アサヒガニ、アブラガニ、タラバガニ、ザリガニ等を用いることができる。The present invention will be described in detail with respect to the most preferred embodiment for carrying out the method of dry sterilization of shrimp head and outer shell and the shellfish dry sterilized powder having the above-described configuration.
If the present invention is a crustacean, lobster, prickly shrimp, tiger shrimp, black tiger, Taisho shrimp, vaname, white, South American pink, bovine shrimp, krill, snow crab, hairy crab, blue crab, Shanghai crab, Asahi crab, abra crab, tarab crab, crayfish etc. Can be used.
上記甲殻類は、乾燥前に天然ミネラルイオン含有パイナップル酵素果汁液を浸漬、含浸して、腐敗防止と加水分解の前処理を実施する。 The crustaceans are immersed in and impregnated with pineapple enzyme juice containing natural mineral ions before drying, and are subjected to pretreatment for anti-corruption and hydrolysis.
本発明の天然ミネラルイオン含有パイナップル酵素果汁液について説明する。ミネラルイオン群を含む鉱物としては、ゼオライト、麦飯石、ヒル石、医王石等を挙げることができる。本発明においては、俗名ヒル石と呼ばれる閃緑岩変質黒雲母に含有されるカルシウム、マグネシウム、カリウム、ナトリウム、鉄、マンガン、アルミニウム、亜鉛、ケイ素、チタン等の金属酸化物の鉱物微細粉をパイナップル酵素果汁液に浸漬してイオン化状態で抽出して得たものを天然ミネラルイオン含有パイナップル酵素果汁液と本発明では称し、還元性を有することを確認している。 The natural mineral ion-containing pineapple enzyme juice of the present invention will be described. Examples of the mineral containing a group of mineral ions include zeolite, barleystone, leechite, and Ioishi. In the present invention, mineral fine powders of metal oxides such as calcium, magnesium, potassium, sodium, iron, manganese, aluminum, zinc, silicon and titanium contained in diorite modified biotite called syllabus leechite are pineapple enzyme What was obtained by immersing in a juice and extracting in an ionized state is referred to as a natural mineral ion-containing pineapple enzyme juice in the present invention, and has been confirmed to have reducibility.
天然ミネラルイオンについて、説明する。
本発明に用いた天然ミネラルイオン含有パイナップル酵素果汁液のミネラル組成値は概して［表２］に示すものを用いた。
次に、パイナップル酵素果汁液について説明する。
パイナップル酵素は、パイナップル皮果汁中に含有することは公知である。パイナップル酵素中にはセルロース分解酵素の他、蛋白質およびアミノ酸分子分解酵素、各種有機酸分解酵素等３０種以上の酵素成分が含まれている。パイナップル酵素は、脱水素（酸化）脱炭酸、脱アミン等の酵素を含んでおり、これらの複合触媒作用により、甲殻類のアミノ酸、蛋白質、脂質、炭水化物等を単分子および低分子に分解する働きがある。Next, the pineapple enzyme juice will be described.
It is known that pineapple enzyme is contained in pineapple peel. In addition to cellulose-degrading enzyme, pineapple enzyme contains 30 or more kinds of enzyme components such as protein and amino acid molecular-degrading enzymes and various organic acid-degrading enzymes. The pineapple enzyme contains enzymes such as dehydrogenation (oxidation) decarboxylation, deamines, etc., and these combined catalysis acts to decompose crustacean amino acids, proteins, lipids, carbohydrates, etc. into single and low molecular weight molecules. There is.
［表３］にパイナップル酵素の主要な成分を示す。
［表３］パイナップル酵素成分の外、プロテアーゼ、アミラーゼ、リパーゼ、キナーゼ、マルターゼ、トランスアミラーゼ、デカルボキターゼ等を用いても差しつかえない。[Table 3] shows the main components of the pineapple enzyme.
[Table 3] In addition to the pineapple enzyme component, protease, amylase, lipase, kinase, maltase, transamylase, decarboxytase, etc. may be used.
本発明の天然ミネラル含有パイナップル酵素果汁液中のミネラルイオン群は、パイナップル酵素と共に甲殻類の有用組成物に含浸吸着され、乾燥すると水酸基を伴った乾燥体を生成し、乾燥体の硬化防止のため必須と成る。 The mineral ions in the natural mineral-containing pineapple enzyme juice of the present invention are impregnated and adsorbed in a useful composition of crustaceans together with the pineapple enzyme, and when dried, a dried body with a hydroxyl group is generated, and the dried body is prevented from hardening. It becomes essential.
続いて、本発明の天然ミネラルイオン含有パイナップル酵素果汁液の腐敗防止作用について説明する。
本発明は、前記した天然ミネラル鉱石の金属酸化物が、パイナップル酵素果汁液に含有する有機酸と共に安定保存のため添加した無機酸に浸漬してイオン状態で抽出する際、生成する励起状態の新生水（Ｈ２Ｏ）が励起水素（Ｈ・）となり、周辺の水（Ｈ２Ｏ）と結合し、プロトニウムイオン（Ｈ３Ｏ＋）（Ｈ・→Ｈ＋ ＋ Ｈ２Ｏ→Ｈ３Ｏ＋）となり、天然ミネラルイオン含有パイナップル酵素果汁液中に長期間安定状態を維持し続け含有される。Subsequently, the anti-rot effect of the natural mineral ion-containing pineapple enzyme juice of the present invention will be described.
The present invention relates to the formation of an excited state when the metal oxide of the natural mineral ore described above is extracted in an ionic state by being immersed in an inorganic acid added for stable storage together with the organic acid contained in the pineapple enzyme juice. Water (H 2 O) becomes excited hydrogen (H.), which is combined with surrounding water (H 2 O), and protonium ions (H 3 O + ) (H · → H + + H 2 O → H 3 O + ) And contained in a natural mineral ion-containing pineapple enzyme juice while maintaining a stable state for a long period of time.
本発明の天然ミネラルイオン含有パイナップル酵素果汁液の腐敗防止作用は、抗酸化と同様この励起状活性水素（Ｈ・）の還元力の働きがあると考えられる。
一般的に励起状の活性水素とは、化学辞典編集委員会「化学大辞典」（共立出版株式会社）によれば、発生期状態の水素は原子状またはそれに近い状態にあり、化学反応を起こしやすくなっている水素を云うと記述されている。
本発明の励起状活性水素についても、原子状または原子状に近い状態であるが、水素ガス（Ｈ２）化することなく、プロトニウムイオン（Ｈ３Ｏ＋）となっていると考えている。It is considered that the anti-corruption action of the natural mineral ion-containing pineapple enzyme juice of the present invention has the function of reducing the excited active hydrogen (H.) as well as the antioxidant.
In general, excited active hydrogen refers to the chemical dictionary editorial committee “Chemical Dictionary” (Kyoritsu Shuppan Co., Ltd.). Hydrogen in the nascent state is in an atomic state or a state close to it, causing a chemical reaction. It is described as referring to hydrogen that is becoming easier.
The excited active hydrogen of the present invention is also in an atomic state or a state close to an atomic state, but is considered to be a protonium ion (H 3 O + ) without being converted to hydrogen gas (H 2 ).
天然ミネラルイオン含有パイナップル酵素果汁液の還元性である励起水素もしくはプロトニウムイオン（Ｈ３Ｏ＋）の存在について金属銅（Ｃｕ）を用いた銅の溶解試験を実施して還元物質の存在を確認した。
銅の溶解は酸化反応を伴いつつイオン化し、青色の銅イオン液となることが知られている。試験は、希硫酸溶液単独では金属銅は溶解しないので、希硫酸溶液に少量の硝酸を添加した硝酸性硫酸溶液中に銅片を入れると、銅片が溶解して青色液になった。
次に、硝酸を添加した硝酸性硫酸溶液中に本発明の天然ミネラルイオン含有パイナップル酵素果汁液を少量添加すると、銅片は溶解することなく、銅片を入れた溶液は無色のままであった。更にこの溶液に鉄片（クギ）を入れたところ、鉄片は錆が発生することなく金属光沢を持ち続けた。
このことは、天然ミネラルイオン含有パイナップル酵素果汁組成中に、酸化を阻止する還元性能を有する励起状水素の存在を示す証拠であると考えている。Existence of excited hydrogen or protonium ion (H 3 O + ), which is a reducing property of pineapple enzyme juice containing natural mineral ions, was subjected to a copper dissolution test using metallic copper (Cu) to confirm the presence of reducing substances. .
It is known that the dissolution of copper ionizes with an oxidation reaction and becomes a blue copper ionic liquid. In the test, since the copper metal was not dissolved by the dilute sulfuric acid solution alone, when the copper piece was put into the nitric acid sulfuric acid solution in which a small amount of nitric acid was added to the dilute sulfuric acid solution, the copper piece was dissolved to become a blue liquid.
Next, when a small amount of the natural mineral ion-containing pineapple enzyme juice of the present invention was added to the nitric acid sulfuric acid solution to which nitric acid had been added, the copper pieces did not dissolve and the solution containing the copper pieces remained colorless. . Furthermore, when iron pieces (kugi) were put into this solution, the iron pieces continued to have a metallic luster without generating rust.
This is considered to be evidence showing the presence of excited hydrogen having a reducing ability to prevent oxidation in the pineapple enzyme fruit juice composition containing natural mineral ions.
本発明の天然ミネラルイオン含有パイナップル酵素果汁液は、前記した励起状活性水素（Ｈ・）が酸化腐敗防止に有効に働くものとの知見で甲殻類の腐敗防止に応用した。
［表１］の天然鉱石の金属酸化物のミネラルイオン化について詳しく説明する。
一例として、ＭｇＯが浸出の際、酸性溶液により、ミネラルイオン化すると共に生成する励起状新生水と励起活性水素（Ｈ・）についての説明を（１）、（２）に示す。
酸性溶液として、パイナップル有機酸のイソ吉草酸〔（ＣＨ３）２ＣＨＣＨＣＯ２Ｈ〕により、ＭｇＯのイオン化とヒドロニウムイオン（Ｈ３Ｏ＋）生成。
ＭｇＯ＋２〔（ＣＨ３）２ＣＨＣＨ２ＣＯＯＨ〕――――→
―――→〔（ＣＨ３）２ＣＨＣＨ２ＣＯＯＨ〕２Ｍｇ２＋＋Ｈ・＋ＯＨ・・・（１）
周辺の水と結合してヒドロニウムイオンとなる。（非特許文献１）。
Ｈ・――――→Ｈ＋＋Ｈ２Ｏ―――→Ｈ３Ｏ＋・・・・・・・・・・・・・・・・（２）
（励起水素） （活性水素） （ヒドロニウムイオン＝プロトニウムイオン）The natural mineral ion-containing pineapple enzyme juice of the present invention has been applied to the prevention of decay of crustaceans based on the knowledge that the above-described excited active hydrogen (H.) works effectively to prevent oxidation and decay.
The mineral ionization of the metal oxides of natural ores in [Table 1] will be described in detail.
As an example, explanations of excited fresh water and excited active hydrogen (H.) generated while mineral ions are ionized with an acidic solution during leaching are shown in (1) and (2).
As an acidic solution, ionization of MgO and formation of hydronium ions (H 3 O + ) with isovaleric acid [(CH 3) 2 CHCHCO 2 H], a pineapple organic acid.
MgO + 2 [(CH 3 ) 2 CHCH 2 COOH] ------->
――― → [(CH 3 ) 2 CHCH 2 COOH] 2 Mg 2+ + H · + OH (1)
Combines with surrounding water to form hydronium ions. (Non-Patent Document 1).
H ・ ―――― → H + + H 2 O- →→ H 3 O + (2)
(Excited hydrogen) (active hydrogen) (hydronium ion = protonium ion)
発明者らは、前記した基礎試験を基に、天然ミネラルイオン含有パイナップル酵素果汁液の還元性物質の励起水素（Ｈ・）捕捉剤ＤＰＰＨ（１，１Ｄｉｐｈｅｎｇｌ−２−ｐｉｏｒｙｌｈｙｄｒａｚｙｌ，ｍａｘ＝５１７ｍｎ）ラジカルスペクトルのピーク減少を利用するＥＳＲ（電子スピン共鳴）法をＥＳＲ測定装置（ＪＯＥＬ製のＪＥＳ−ＦＥ３ＸＧ Ｓｐｅｏｔｒｏｍｅｔｅｒ）〔（株）ウォーターデザイン研究所（城西大学薬学部）〕により、更に詳しい測定を実施して、還元性物質が原子状の活性水素（Ｈ・）であることを確認した。
発明の励起水素（ラジカル水素Ｈ・）の検出方法は、次の反応に基づきＤＰＰＨラジカルが還元性能を有するＨ・を捉えると、定量的に反応しＤＰＰＨラジカルピーク（５１７ｎｍ）が消失する原理を利用した検出方法である。
反応式を式［化１］およびＳｉｇｎａｌ ｉｎｔｅｎｓｉｔｙ（Ｉ）ｈ２／ｈ１図を［図１］に示す。Based on the basic test described above, the inventors of the present invention have demonstrated that the reducing substance of the natural mineral ion-containing pineapple enzyme juice is an excited hydrogen (H.) scavenger DPPH (1,1 Diphengl-2-piorhydrohydrazyl, max = 517 mn) radical spectrum. The ESR (electron spin resonance) method using the peak reduction of the ESR is measured by an ESR measurement device (JES-FE3XG Spetrometer, manufactured by JOEL) [Water Design Laboratory (School of Pharmacy)], It was confirmed that the reducing substance was atomic active hydrogen (H.).
The method for detecting excited hydrogen (radical hydrogen H.) of the invention uses the principle that DPPH radical (517 nm) disappears quantitatively when DPPH radical captures H. which has reducing performance based on the following reaction. Detection method.
The reaction formula is represented by the formula [Chemical Formula 1] and the signal intensity (I) h2 / h1 diagram is shown in FIG.
反応式（３）は、天然ミネラルイオン含有パイナップル酵素果汁液５００倍希釈溶液を用いた場合について、励起状活性水素（Ｈ・）とＤＰＰＨが反応しＤＰＰＨ−Ｈを生成し、ＤＰＰＨラジカルピークの減少（ｈ１／ｈ２）［図１］を示した検出ピーク図である。
ＤＰＰＨと反応した励起活性水（Ｈ・）量は２０．０ｍｇＨ／Ｌを検出した。Reaction formula (3) shows that when a 500-fold diluted solution of pineapple enzyme juice containing natural mineral ions is used, excited active hydrogen (H.) reacts with DPPH to produce DPPH-H, and the DPPH radical peak decreases. (H1 / h2) FIG. 1 is a detection peak diagram showing [FIG. 1].
The amount of excited active water (H ·) reacted with DPPH was detected as 20.0 mgH / L.
本発明の甲殻類の過熱蒸気乾燥殺菌処理方法について説明する。
過熱蒸気乾燥殺菌装置（株式会社エコノス・ジャパン社製）型式：スーパースチーマミニ試験機を用い、甲殻類のエビの頭部および外殻を天然ミネラルイオン含有パイナップル果汁酵素液に浸漬・含浸した後、これを装置内部に挿入し、過熱蒸気温度１６０℃〜１８０℃に保持しつつ過熱蒸気乾燥殺菌処理して、甲殻類の乾燥殺菌した後、小型高速粉砕機（三庄インダストリー製）型式：ＮＨ−３４型を用いて甲殻類の乾燥殺菌粉末を得る。The superheated steam drying sterilization method for crustaceans of the present invention will be described.
Superheated steam drying sterilizer (Econos Japan Co., Ltd.) model: After using the Super Steamer Mini tester, the crustacean shrimp head and outer shell were immersed and impregnated in natural mineral ion-containing pineapple juice enzyme solution, This was inserted into the apparatus, and superheated steam drying sterilization treatment was carried out while maintaining the superheated steam temperature at 160 ° C. to 180 ° C., and crustaceans were dried and sterilized. Then, a small high-speed pulverizer (manufactured by Sansho Industry) Model: NH- Crustacean dry sterilized powder is obtained using type 34.
以下、実施例により、更に詳しく本発明の説明を記述するが、これら実施例は本発明を限定するものではない。 Hereinafter, the present invention will be described in more detail with reference to examples, but these examples do not limit the present invention.
廃棄甲殻類のエビの頭部１００重量部、天然ミネラルイオン含有パイナップル酵素果汁液５０倍希釈液５０重量部を浸漬容器に投入、常温にて攪拌、浸漬１２０分した後、該天然ミネラルイオン含有パイナップル酵素果汁液含浸エビの頭部を収容器に移し、１６０℃〜１８０℃に設定された過熱蒸気乾燥殺菌処理装置に挿入し、１８分〜２０分間過熱蒸気乾燥殺菌処理を実施し、乾燥殺菌処理したエビの頭部２０重量部を得た。 Disposable crustacean shrimp head 100 parts by weight, natural mineral ion-containing pineapple enzyme juice 50-fold diluted solution is put into an immersion container, stirred at room temperature and immersed for 120 minutes, then the natural mineral ion-containing pineapple Move the head of the shrimp impregnated with enzyme juice into a container, insert it into a superheated steam drying sterilization treatment device set at 160 ° C to 180 ° C, implement a superheated steam drying sterilization treatment for 18 to 20 minutes, and dry sterilization treatment Shrimp head 20 parts by weight was obtained.
［実施例１］に用いたエビの頭部を天然ミネラルイオン含有パイナップル酵素果汁液を無添加のまま、周知の乾燥装置により乾燥処理し、食品粉砕機にて比較例乾燥エビ粉末を得た。 The shrimp head used in [Example 1] was dried with a known drying apparatus without adding the natural mineral ion-containing pineapple enzyme juice, and a comparative example dried shrimp powder was obtained with a food grinder.
［実施例１］および［比較例１］で得た乾燥殺菌エビ粉末および通常乾燥処理エビ粉末について、アミノ酸および有用成分の成分組成の定量分析を（社）日本食品分析センター（前記）により実施した。結果を［表４］［表５］に示す。 About the dry sterilized shrimp powder obtained in [Example 1] and [Comparative Example 1] and the normally dried shrimp powder, quantitative analysis of the component composition of amino acids and useful components was conducted by the Japan Food Analysis Center (supra). . The results are shown in [Table 4] [Table 5].
［表４］［表５］より、本発明の［実施例１］の結果、過熱蒸気乾燥殺菌処理した乾燥殺菌エビ粉末は、低分子アミノ酸２０種が高い含有率で含有され、比較例の１０００倍近い値を示し、且つ、有用成分の蛋白質の値は低下し、脂質、炭水化物を高い含有率として得ることができた。蛋白質が低下し、アミノ酸各種が増加していることは、比較例に対して実施例では、蛋白質が低分子化されアミノ酸の低分子に変化している証である。
即ち、分子量（Ｍ，Ｗ）１７０以下の低分子アミノ酸を高く含有する、グルタミン（Ｍ，Ｗ１４６．２）、グルタミン酸（Ｍ，Ｗ１４７．１）、Ｌ−アスパラギン（Ｍ，Ｗ１３２．１）、Ｌ−アスパラギン酸（Ｍ，Ｗ１３３．１）、Ｌ−プロリン（Ｍ，Ｗ１１５．１）、グリシン（Ｍ，Ｗ７５．０７）、アラニン（Ｍ，Ｗ８９．０９）、バリン（Ｍ，Ｗ１１７．２）、ロイシン（Ｍ，Ｗ１３１．２）、リジン（Ｍ，Ｗ１５５．２）、フェルアラニン（Ｍ，Ｗ１６５．３）等、含有値３．００ｇ／１００〜６．００ｇ／１００ｇを得た。
更に、比較例の通常乾燥処理粉末では検出されないアミノ酸成分であるリジン、ヒスチジン、フェルアラニン、メチオニン、グリシン、セリン、スレオニン、トリプトファン等が、本発明の天然ミネラル含有パイナップル酵素果汁浸漬・含浸後、過熱蒸気乾燥殺菌処理エビ粉末中には高い含有値で検出された。From [Table 4] and [Table 5], as a result of [Example 1] of the present invention, the dry sterilized shrimp powder subjected to the superheated steam dry sterilization treatment contains 20 kinds of low molecular weight amino acids at a high content rate. The value of the protein as a useful component was lowered and the lipid and carbohydrate content could be obtained at a high content. The decrease in protein and the increase in various amino acids is a proof that the protein is reduced in molecular weight to a low molecular weight amino acid in the examples compared to the comparative example.
That is, glutamine (M, W146.2), glutamic acid (M, W147.1), L-asparagine (M, W132.1), L- containing high molecular weight (M, W) 170 or less low molecular amino acids. Aspartic acid (M, W133.1), L-proline (M, W115.1), glycine (M, W75.07), alanine (M, W89.09), valine (M, W117.2), leucine ( M, W131.2), lysine (M, W155.2), feralanine (M, W165.3), etc., content values of 3.00 g / 100 to 6.00 g / 100 g were obtained.
Furthermore, lysine, histidine, ferlanine, methionine, glycine, serine, threonine, tryptophan, etc., which are amino acid components that are not detected in the normally dried powder of the comparative example, are heated after soaking and impregnating the natural mineral-containing pineapple enzyme juice of the present invention. A high content value was detected in steam-dried sterilized shrimp powder.
［実施例１］および［比較例１］で得た、それぞれのエビ粉末１００重量部を常法により抽出液（エタノール６０％、水４０％）３５０重量部に投入し６０℃にて４０分間密閉状態で攪拌し成分抽出後、更に抽出した液を８０℃まで加熱しエタノールを除去した後の成分抽出溶液１４０重量部を得た。更に得た抽出液を８０℃にて５０分間加熱して濃縮液１２０重量部を得た。 100 parts by weight of each shrimp powder obtained in [Example 1] and [Comparative Example 1] are put into 350 parts by weight of an extract (ethanol 60%, water 40%) by a conventional method and sealed at 60 ° C. for 40 minutes. After stirring and stirring for component extraction, the extracted liquid was further heated to 80 ° C. to remove ethanol to obtain 140 parts by weight of component extraction solution. Furthermore, the obtained extract was heated at 80 ° C. for 50 minutes to obtain 120 parts by weight of a concentrated solution.
［実施例３］方法で得た、［表４］［表５］の実施例および比較例で得たエビ粉末の液体抽出液について、アミノ酸および有用成分の成分濃度の定量分析を（社）日本食品分析センター（前記）により分析実施した。
結果を［表６］［表７］に示す。[Example 3] Quantitative analysis of amino acid and useful component concentrations of shrimp powder liquid extracts obtained in Examples and Comparative Examples of [Table 4] and [Table 5] obtained by the method was conducted in Japan. Analysis was performed by the Food Analysis Center (supra).
The results are shown in [Table 6] and [Table 7].
［表６］［表７］より［実施例３］で得たエビ粉末抽出液のアミノ酸成分分析の結果、本発明の実施例で得た乾燥殺菌エビ粉末に含有される低分子アミノ酸は、その９６％以上が抽出され離分子アミノ酸総合含有量４２．３ｇ／１００ｇとなり、低分子アミノ酸の高濃度の抽出液組成値を得ることができた。
即ち、抽出液１００ｇ中アミノ酸であるグルタミン３．４２ｇ、グルタミン酸５．４４ｇ、アスパラギン２．５１ｇ、アスパラギン酸３．７ｇ、プロリン３．０１ｇ、グリシン２．７８ｇ、アラニン２．５８ｇ、フェルアラニン２．１５ｇ、バリン２．３３ｇ、ロイシン２．１５ｇ、リジン２．８２ｇ他、総アミノ酸４２．３ｇを含有することが検出された。その抽出液の一般細菌、大腸菌、黄色ブドウ球菌および耐熱性芽胞菌数は全て陰性ないし検出せずであった。[Table 6] As a result of the amino acid component analysis of the shrimp powder extract obtained in [Example 3] from [Table 7], the low molecular weight amino acid contained in the dry sterilized shrimp powder obtained in the example of the present invention is More than 96% was extracted, and the total molecular weight of amino acid residues was 42.3 g / 100 g, and a high concentration extract composition value of low molecular amino acids could be obtained.
That is, the amino acid glutamine 3.42g, glutamic acid 5.44g, asparagine 2.51g, aspartic acid 3.7g, proline 3.01g, glycine 2.78g, alanine 2.58g, feralanine 2.15g in 100g of extract. , 2.33 g of valine, 2.15 g of leucine, 2.82 g of lysine, and 42.3 g of total amino acids were detected. The numbers of general bacteria, Escherichia coli, Staphylococcus aureus and thermostable spore bacteria in the extract were all negative or not detected.
本発明は、廃棄甲殻類のエビの頭部および外殻に天然ミネラルイオン含有パイナップル酵素果汁液を浸漬・含浸させた後、過熱蒸気乾燥殺菌処理して、粉砕したエビ粉末は、低分子アミノ酸の高含有率を示し、該エビ粉末は通常抽出法により、低分子アミノ酸の高濃度抽出液を得ることを可能にした。これより、食品添加物、健康食品、化粧品、医薬品、各飼料、ペットフード、肥料等への利用性への可能性が高い。 In the present invention, the shrimp powder pulverized by soaking and impregnating pineapple enzyme juice containing natural mineral ions in the crust head and outer shell of waste crustacean, followed by superheated steam drying sterilization, The shrimp powder showed a high content, and it was possible to obtain a high concentration extract of low molecular amino acids by the usual extraction method. From this, the possibility to use for food additives, health foods, cosmetics, pharmaceuticals, feeds, pet foods, fertilizers, etc. is high.
［図１］に基づく符号
（１）ＤＰＰＨ（１−１−Ｄｉｐｈｅｙｌ−２−ｐｉｃｒｙＬｈｙｄｅａｚｙｌ，λｍａ ｘ＝５１７ｎｍ）
フリーラジカル
（２）Ｈ・（励起状活性水素）
（３）ＤＰＰＨ−Ｈ ＤＰＰＨフリーラジカルのＮ部に、Ｈ・が捕捉されＤＰＰＨ−Ｈが 生成された分子構造式。
（Ａ）ＥＳＲＤＰＰＨ Ｓｉｇｎａｌ（ｈ１）
（Ｂ）ＥＳＲＤＰＰＨ−Ｈ Ｓｉｇｎａｌ（ｈ２）
（ｈ２／ｈ１＜ １）：ＥＳＲ Ｓｉｇｎａｌ ｉｎｔｅｎｓｉｔｙ（Ｉ）
（Ｈ・捕捉によりＳｉｇｎａｌ ｉｎｔｅｎｓｉｔｙ（Ｉ）は１以下となる。）Code based on [FIG. 1] (1) DPPH (1-1-Diphenyl-2-picryLhydeazil, λmax = 517 nm)
Free radical (2) H. (Excited active hydrogen)
(3) DPPH-H Molecular structural formula in which DP · H is trapped in the N part of the DPPH free radical to generate DPPH-H.
(A) ESRDPPH Signal (h1)
(B) ESRDPPH-H Signal (h2)
(H2 / h1 <1): ESR Signal intensity (I)
(Signal intensity (I) is 1 or less due to H-trapping.)
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| CN115280949A (en) * | 2022-09-05 | 2022-11-04 | 顺祥食品有限公司 | Fertilizing method for direct-seeding rice |
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