JP2011157350A - Liquid composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide therapeutic agents for diabetes, hemorrhoids, trichophytosis unguium, colon polyps and the like. <P>SOLUTION: A liquid composition is prepared by impregnating absorbent cotton having about 1 cm×1 cm size with wood vinegar (e.g. bamboo vinegar) containing ≤1 ppt weight benzopyrene and is used by inserting the same into the anus. Thereby, the bactericidal action inherited to the bamboo vinegar is directly acted to the affected part, as well as imparts bactericidal function to blood flow through inner organs such as liver and expresses bactericidal effects from the internal organs and the insides of peripheral parts of the body to be able to improve symptoms such as trichophytosis unguium and colon polyps. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a wood vinegar solution useful for use as a therapeutic agent for diabetes, hemorrhoids, nail fungus, colon polyps, etc., using the bactericidal power of wood vinegar solution such as bamboo vinegar and the annihilation action of antibodies of insulin receptor. Is.

Wood vinegar is the supernatant of dry-distilled liquid that is produced when wood is carbonized. As a typical example, wood vinegar is produced as a by-product during charcoal baking. In the present specification, wood means not only general wood such as bamboo and pine but also general plants including flowers such as vines.
Bamboo vinegar, which is made from bamboo, is a liquid component that is generated when bamboo charcoal is produced by dry distillation. About 90% (% by weight, the same shall apply hereinafter) is water, about 5% is acetic acid, and other alcohol. About 200 kinds of organic machine components such as alcohols, esters and phenols are included.

  In addition, as an example of the application using the bactericidal action of acetic acid and organic acid compounds contained in bamboo vinegar, Patent Document 1 includes applying a cream-like composition containing bamboo vinegar to the affected area. It has been disclosed that it is effective as a therapeutic agent for atopic dermatitis and nail fungus.

  Patent Document 2 discloses that a bamboo vinegar solution from which benzpyrene, which is a carcinogenic substance, has been removed is added to food and drink and orally ingested, so that therapeutic effects and symptoms of arteriosclerosis, diabetes, and hay fever are obtained. It is disclosed that an improvement effect can be obtained.

  In addition to the above, chemotherapy to deal with various diseases by oral administration of drugs is performed, but these normalize the state of the large intestine, and antibodies are generated at receptors due to infection such as viruses. This is an improvement method aimed at normalizing the functions of each part of the living body by improving the action of the living body and increasing the metabolism of the living body.

  However, since the above-mentioned drug administration method is oral administration, it hardly affects the affected area or mucous membrane directly. Therefore, the concentration of benzpyrene is not strictly regulated. For example, Patent Document 2 states that “bamboo vinegar is extremely safe without the inclusion of harmful substances such as benzpyrene, which is a carcinogenic substance,” but there is no specific concentration of benzpyrene. The actual concentration of benzpyrene is well over several tens of ppt.

  When administered from the anus, unlike the oral or epidermal administration, the composition acts directly on the affected area or mucous membrane, so the content of benzpyrene, which is a side effect component, must be reduced as much as possible. However, at the present time, no technology has been proposed in which Benzpyrene is set to 1 ppt or less.

Japanese Patent Laid-Open No. 2005-200393 JP 2004-141141 A

  Therefore, an object of the present invention is to provide a therapeutic effect for diabetes, hemorrhoids, nail fungus, colon polyps, etc. that can be carried out at home using a natural vinegar solution that is an inexpensive natural material with extremely few side effects and is safe for the human body. It is providing the liquid composition which can be exhibited.

  The invention according to claim 1 is a liquid composition characterized in that the content of benzpyrene consists of a wood vinegar solution of 1 ppt (weight ppt, the same shall apply hereinafter) or less and is administered through the anus.

The invention according to claim 2 is the liquid composition according to claim 1, which is used as a therapeutic agent for diabetes that kills roundworms and Helicobacter pylori in the large intestine and extinguishes insulin receptor antibodies.
Invention of Claim 3 is a liquid composition of Claim 1 used for the use as a therapeutic agent of the hemorrhoid which makes the bactericidal action of a wood vinegar liquid act on an affected part directly.
The invention according to claim 4 is the liquid composition according to claim 1, which is used as a therapeutic agent for nail athlete's foot or colon polyp which imparts the bactericidal action of wood vinegar to the bloodstream without passing through the portal vein.
The invention according to claim 5 is the liquid composition according to any one of claims 1 to 4, wherein the wood vinegar is bamboo vinegar.

  As described above, the present invention inserts cotton soaked in wood vinegar into the anus and administers the active ingredient of bamboo vinegar having bactericidal action directly to the affected area, and after being absorbed from the rectum, the liver Since it is a method of giving to the blood flow of the whole body via the, it has an excellent effect that could not be obtained by conventional treatment methods.

  In addition, because patients can use themselves and there are few side effects, there is an increase in adult diseases due to the recent shift to dietary habits in Europe, the increasing economic burden on patients, and the growing shortage of doctors. It is possible to deal with these problems.

Specifically, the sterilization function of bamboo drunk or pyroligneous acid acts directly on the affected area of the heel and acts on the nail athlete's foot and colon polyps via the bloodstream.
Diabetes type B insulin resistance, in which Helicobacter pylori, which was thought to cause peptic ulcers such as gastritis and gastric / duodenal ulcers, creates antibodies that interfere with the action of insulin that lowers blood glucose level In recent years, it has been clarified that the sterilization effect of bamboo drunk or pyroligneous acid is effective in killing Helicobacter pylori, and also has therapeutic effects on B-type insulin resistance. There is.
Since benzpyrene is 1 ppt or less, it can be used as the therapeutic agent without having to consider side effects such as carcinogenesis caused by this.

It is the photograph which image | photographed the state of the nail moth before administration of bamboo drunk. This is a photograph of the condition of the nail fungus after the bamboo vinegar solution has been repaired for 4 months. It is the photograph which image | photographed the state of the nail athlete's foot after continuously administering bamboo vinegar for 9 months. 1 is a conceptual diagram of a liquid chromatography system. It is the schematic of the bamboo vinegar liquid refinement | purification system by the column method. It is an external appearance photograph before and after refinement | purification of a bamboo vinegar liquid. It is a liquid chromatogram of the bamboo vinegar liquid sample C after refinement | purification. It is a GC-MS chromatogram before and after ODS silica purification of bamboo vinegar sample C, (A) shows before purification and (B) shows after purification. It is an endoscopic photograph of the intestine before bamboo vinegar application in an Example. FIG. 10 is a conceptual diagram of the intestine showing the examination unit in FIG. 9. It is an endoscopic photograph of the intestine after bamboo vinegar application in an Example. It is a conceptual diagram of the intestine which shows the test | inspection part in FIG.

Next, an embodiment of the present invention will be described.
Three types of commercially available bamboo vinegar were prepared. The concentration of benzpyrene is as follows.
Sample A: Large amount Sample B: 10 ppb (10 μg / L)
Sample C: 60 ppt (60 ng / L)
The concentration of benzpyrene was analyzed using the liquid chromatography system shown in FIG.
A more detailed analysis apparatus and analysis conditions are as follows.
(Analysis equipment)
Liquid feed pump: LC-20AD (Shimadzu Corporation)
Autosampler: SIL-20A / 20AC (Shimadzu Corporation)
Column oven: CTO-20A / 20AC (Shimadzu Corporation)
Detector: RF-20A (Shimadzu Corporation)

(Analysis conditions)
Analysis column: VP-ODS (150 mm × 4.6 mm.d.)
Mobile phase: acetonitrile / 10 mM phosphate buffer = 8/2
Flow rate: 0.8 mL / min, Isocratic mode
Evaluation temperature: 40 ° C
Detection: fluorescence detection, excitation wavelength -295 nm, fluorescence wavelength -403 nm

Benzpyrene in bamboo vinegar was removed by the following operations using the reagents described below for Samples A, B, and C.
(reagent)
・ Acetonitrile (Wako Pure Chemical Industries)
・ Methanol (Wako Pure Chemical Industries)
・ Pure water (Milli-Q pure water production equipment)
· ODS silica (Cosmosil _140C 18 -0PN, Nacalai Tesque)
* ODS silica (octadecyl group-bonded silica gel) used for evaluation has an average particle size of 140 μm. Its surface is hydrophilic. This ODS silica can be used in an open column.

(operation)
The concept of the bamboo vinegar liquid refinement | purification procedure by the column method is shown in FIG.
1) A slurry was prepared by dispersing 20 g of ODS silica in 100 mL of a 30% acetonitrile aqueous solution.
2) The slurry was filled in a glass column with an inner diameter of 25 mm filled with absorbent cotton and sea sand at one end.
3) As conditioning, 100 mL each of methanol and water were passed to activate ODS silica.
4) Commercially available bamboo vinegar liquid samples A, B, and C were passed through 100 mL each, and the passing liquid was collected.
5) In order to measure the concentration of benzpyrene in the recovered liquid, each recovered liquid was analyzed by liquid chromatography / fluorescence detection.

(result of analysis)
-Visible change As a visible change of the sample before and after purification, the state before and after purification of the bamboo vinegar sample A is shown in FIG. As is apparent from these photographs, it can be seen that the coloration derived from the tar component is almost completely removed before and after purification. Similarly, the odor peculiar to bamboo vinegar has almost disappeared. From this, tar components and the like containing benzpyrene were efficiently removed.
-Regarding the evaluation of the acidity before and after the pH change purification treatment, no pH change was observed before and after purification in all samples. For example, in the commercially available bamboo vinegar liquid sample A, the pH before purification was 2.62, whereas it was 2.65 after purification. This result means that the organic acid component containing acetic acid, which is the main component, is not removed before and after purification, suggesting that an organic acid having antibacterial properties remains.
-Liquid chromatography The final chromatogram of the commercially available bamboo vinegar sample C after purification is shown in FIG. As shown in this figure, the peak of benzpyrene cannot be confirmed, and benzpyrene in the bamboo vinegar is reduced to 1 ppt or less by the ODS silica column method.
Moreover, about reproducibility evaluation, as a result of repeating experiment of the commercially available bamboo vinegar liquid sample C, benzpyrene is not confirmed by any sample, and it can be judged that the reproducibility of this method is favorable.

-Components other than Benzpyrene Components other than Benzpyrene were also analyzed.
The analysis conditions are as follows.
Analysis conditions GC-MS apparatus: Agilent 5973
Capillary column using Vent-free GC / MS adapter: UA5-15M-0.25F (5% polydiphenylsiloxane)
(15m, 0.25mm, 0.25micron)
Sample injection volume: 1 μL
Injector: Split / split less injector-300 ° C., split ratio: 1/30
GC / MS interface: 300 ° C
MS ion source: 250 ° C
FIG. 8 shows the results of GC-MS chromatogram analysis of sample C before and after purification.
As can be seen from FIG. 8, butylactone, phenolic, guaiacol, syringol and the like contained in the commercially available bamboo vinegar liquid sample C are completely removed. On the other hand, since a very large peak in the vicinity of 1 minute containing acetic acid as a main component is hardly changed, not only benzpyrene but also an aromatic compound having the same toxicity is removed by the ODS silica treatment. It became clear.
For confirmation, polycyclic aromatics contained in the purified bamboo vinegar solution were evaluated using high-sensitivity GC-MS. An unpurified sample of commercially available bamboo vinegar liquid sample C and its ODS silica purified product (column method) were used for evaluation. As a result of detailed analysis, in the unpurified bamboo vinegar sample C, tens pyrt of tens of ppt was confirmed as in the fluorescence analysis. On the other hand, in the sample after purification of ODS silica, no major polycyclic aromatics including benzpyrene were detected at all. From these results, benzpyrene removal was proved also in other analysis methods, and a bamboo vinegar solution reduced to less than 1 ppt by a column method using ODS silica could be prepared.

(Dosage test)
An administration test to humans was conducted on the bamboo vinegar liquid purified by the above procedure.
The bamboo vinegar solution used was obtained by purifying the sample C described above to a benzpyrene concentration of 1 ppt or less.
As described above, the method of using the bamboo vinegar in this example is to impregnate a material that absorbs and holds a liquid such as absorbent cotton and insert it into the anus. Specifically, absorbent cotton cut into a diameter of about 1 cm was impregnated with 0.25 mg to 1 mg of the refined bamboo vinegar solution, and administered twice daily after defecation and after bathing.

[Lung function / liver function]
Table 1 shows daily measurements of liver function, kidney function, etc. before the start of administration and after 6 months of administration at Miyagi Medical Association Health Center, which is an analysis institution, every day for 6 months. It is the result.
Here, each numerical value and the rate of improvement are shown.

  As is clear from the numerical values shown in Table 1, the numerical values indicating lung function and renal function are both improved after administration than before administration, and the results of the administration of bamboo vinegar are recognized. became.

[Nail athlete's foot]
Similarly, the therapeutic effect on nail fungus was also examined. In this case, the effect appeared after continuous administration for 9 months. FIG. 1 is a photograph of the state of nail athlete's foot before administration of bamboo vinegar, FIG. 2 is a photograph of the condition of nail athlete's foot after continuous administration of bamboo vinegar for 4 months, and FIG. 3 is bamboo vinegar. It is the photograph which image | photographed the state of the nail fungus after continuously administering a liquid for 4 months.
The results shown in these figures are considered to be because the bactericidal effect of the therapeutic agent of this example reached the end of the body by the whole body blood flow without passing through the portal vein. It also has the effect of producing a therapeutic effect without being affected by liver metabolism and without receiving the first-pass effect.

  In addition, when bamboo vinegar is administered as described above, the anus and lower abdomen during a stool can be refreshed, abdominal pain and abdominal feeling disappeared, and the hardness of the stool can maintain a state that does not interfere with the stool. It can be seen that the bactericidal action of bamboo vinegar is effective. The hall that felt pain and itching around the anus before administration resolved those symptoms, and a therapeutic effect on the fistula was also observed.

  As described above, according to the present invention, it is possible to provide a therapeutic agent that has a large effect on nail athlete's foot by administration of bamboo vinegar from the anus and has few side effects. The contribution is extremely large and useful.

[Colon polyp examination]
The effect on the intestine was examined in FIGS.
FIG. 9 shows an endoscopic photograph of the intestine before administration. FIG. 10 shows the inspection location. 1-8 is an inspection location, and a polyp is found at a position indicated by an arrow 1-4 as shown in FIG.
The test results after the above administration for 6 months are shown in FIG. The inspection location is 1-4 in FIG. As shown in FIG. 11, no polyp was found at 1-4.

Claims (5)

A liquid composition comprising a vinegar solution of benzpyrene having a content of 1 ppt (weight ppt, the same shall apply hereinafter) or less, and being administered through the anus. The liquid composition according to claim 1, which is used for use as a therapeutic agent for diabetes which kills roundworms and Helicobacter pylori in the large intestine and extinguishes insulin receptor antibodies. The liquid composition of Claim 1 used for the use as a therapeutic agent of the hemorrhoid which makes the bactericidal action of wood vinegar act on an affected part directly. The liquid composition according to claim 1, which is used as a therapeutic agent for nail athlete's foot or colon polyp which imparts the bactericidal action of wood vinegar to the bloodstream without passing through the portal vein. The liquid composition according to any one of claims 1 to 4, wherein the wood vinegar is bamboo vinegar.

Images