JP2011092216A - Method for production of softened fish meat and meat - Google Patents

Method for production of softened fish meat and meat Download PDF

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JP2011092216A
JP2011092216A JP2011029092A JP2011029092A JP2011092216A JP 2011092216 A JP2011092216 A JP 2011092216A JP 2011029092 A JP2011029092 A JP 2011029092A JP 2011029092 A JP2011029092 A JP 2011029092A JP 2011092216 A JP2011092216 A JP 2011092216A
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meat
fish
enzyme
treatment
livestock
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Hiroaki Konishi
宏明 小西
Keiji Iohara
啓司 庵原
Tomoko Fujimoto
知子 藤本
Ken Mitomi
健 三富
Toshiyuki Kasai
俊之 葛西
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Maruha Nichiro Foods Inc
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Maruha Nichiro Foods Inc
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for production of softened fish meat and meat suppressing excessive softening of surface and collapse of shape such as collapse of material, giving soft fish meat and meat with delicious appearance. <P>SOLUTION: The fish meat and meat satisfy the Universal design food class 2 standard (physical properties set as upper limit value of hardness: 5×10<SP>4</SP>N/m<SP>2</SP>) of the Japan Care Food Conference, by subjecting the fish meat or the meat to physical damage by tenderizing, together with an enzymatic reaction using an enzyme soaking technique such as vacuum treatment and tumbling. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

素材の形状を維持し、見た目もおいしく、やわらかい魚肉・畜肉の製造方法に関する。   The present invention relates to a method for producing soft fish meat and livestock meat that maintains the shape of the material and looks delicious.

テンダライズ法(加工対象素材に針状の刃を刺し通し、原形を保ったまま硬い筋や繊維を短く切断する処理)や高圧処理法(レトルト処理)など、物理的な処理を加えて肉を軟化させる方法が知られているが、これらの方法による加工処理では、いずれも日本介護食品協議会が定めるユニバーサルデザインフードの区分2(物性規格は硬さ上限値が5×104 N/m2)を満たすやわらかさを達成できない場合がある。 Meat is softened by applying physical processing such as tenderizing (processing to insert a needle-like blade into the material to be processed and cutting hard streaks and fibers while keeping the original shape) and high-pressure processing (retort processing). There are known methods, but in these processing methods, universal design food category 2 defined by the Japan Nursing Food Council (the physical property standard has a hardness upper limit of 5 × 10 4 N / m 2 ) You may not be able to achieve the softness that meets your requirements.

酵素を用いて軟化させる方法として、酵素液に浸漬する方法(特許文献1)、加圧処理して酵素を浸透させる方法(特許文献2)、酵素液をインジェクションする方法(特許文献3、特許文献4)、酵素液をインジェクションしてタンブリング(酵素液を機械的に浸透する処理)する方法(特許文献5)、等が既に提案されている。しかし、何れも表面の過度の軟化やインジェクションによる身割れなどの問題(素材の崩壊)を生じる場合がある。   As a method of softening with an enzyme, a method of immersing in an enzyme solution (Patent Document 1), a method of infiltrating an enzyme by pressure treatment (Patent Document 2), and a method of injecting an enzyme solution (Patent Document 3, Patent Document) 4) A method (Patent Document 5) for injecting an enzyme solution to perform tumbling (a treatment for mechanically penetrating the enzyme solution) has already been proposed. However, in any case, problems such as excessive softening of the surface and cracking due to injection (disintegration of the material) may occur.

テンダライズ法と酵素を併用する方法として、(株)阿部亀商店の「軟化加工魚肉の製造方法」(特許文献6)がある。「魚肉に至適温度が40℃以上のプロテアーゼを10℃以下の温度で浸透させる第1工程と、その後生肉中でプロテアーゼを活性化させて所定時間保つ第2工程とを有する。」ことを特徴とし、「第一工程の前又は間に、生肉に針をあけること」が記載されている。しかし、特許文献6には、ユニバーサルデザインフードの区分2を満たすやわらかさを達成するという技術課題及びそれを達成するための技術についての開示はない。更に、引用文献6には、上記の酵素処理に対して減圧処理やタンブリング処理を組み合わせることに関する記載はない。   As a method of using the tenderizing method and an enzyme in combination, there is "A method for producing softened processed fish meat" (Patent Document 6) by Abe Kame Shoten Co., Ltd. “It has a first step of allowing a protease having an optimum temperature of 40 ° C. or more to penetrate into fish meat at a temperature of 10 ° C. or less, and then a second step of activating the protease in raw meat and keeping it for a predetermined time”. And “open a needle in raw meat before or during the first step”. However, Patent Document 6 does not disclose a technical problem of achieving a softness that satisfies the category 2 of the universal design hood and a technique for achieving the technical problem. Furthermore, the cited document 6 does not describe the combination of the reduced pressure process and the tumbling process with the above enzyme process.

減圧処理して酵素液を浸漬させる方法として、広島県「熟成食品の製造方法」(特許文献7)がある。「減圧処理(食品素材の膨張)により酵素を導入する工程と圧縮工程とを有する熟成食品の製造方法」に関する出願であるが、減圧下で食品素材を膨張させて酵素を導入してから、食品素材を元の食品素材の体積より小さい体積に圧縮して酵素を作用させる点に特徴を有するものである。特許文献7にも、ユニバーサルデザインフードの区分2を満たすやわらかさを達成するという技術課題及びそれを達成するための技術についての開示はない。更に、特許文献7には、上記の酵素処理に対しするデンダライズ等の物理的処理の組合せに関する記載はない。また、本願発明者による検討では、特許文献7に記載される減圧処理だけではユニバーサルデザインフードの区分2を満たすレベルのやわらかさまで到達できなかった。   As a method of immersing an enzyme solution by performing a reduced pressure treatment, there is a “Preparation Method of Aged Food” in Hiroshima Prefecture (Patent Document 7). Although it is an application related to “a method for producing an aged food having a step of introducing an enzyme by decompression treatment (expansion of food material) and a compression step”, the food is introduced after expanding the food material under reduced pressure to introduce the enzyme. It is characterized in that the material is compressed to a volume smaller than that of the original food material and the enzyme is allowed to act. Patent Document 7 also does not disclose a technical problem of achieving a softness that satisfies Category 2 of the universal design hood and a technique for achieving the technical problem. Furthermore, Patent Document 7 does not describe a combination of physical treatments such as denrization for the enzyme treatment. Further, as a result of the study by the inventors of the present application, it was not possible to reach the softness of the level satisfying the category 2 of the universal design hood only by the decompression process described in Patent Document 7.

特開平7-31421号公報JP-A-7-31421 特開2004-89181号公報JP 2004-89181 A 特開平11-346718号公報Japanese Patent Laid-Open No. 11-346718 特開2008-125437号公報JP 2008-125437 A 特表2005-503172号公報JP 2005-503172 Publication 特開2004-275083号公報Japanese Patent Laid-Open No. 2004-275083 特開2009-89668号公報JP 2009-89668

本発明の目的は、表面の過度の軟化や見割れなどによる形状崩壊を抑制し、見た目もおいしく、やわらかい魚肉や畜肉の製造方法を提供することにある。そのやわらかさは、日本介護食品協議会が定めるユニバーサルデザインフードの区分2(物性規格は硬さ上限値が5×104 N/m2)を満たし、歯ぐきでつぶせるやわらかさである。 An object of the present invention is to provide a method for producing soft fish meat and livestock meat that suppresses shape collapse due to excessive softening or cracking of the surface, is delicious, and is soft. The softness meets the category 2 of the universal design food set by the Japan Nursing Food Council (the physical property standard has a hardness upper limit of 5 × 10 4 N / m 2 ) and is soft enough to be crushed with a gum.

本発明にかかる軟化魚肉または畜肉製品の製造方法は、軟化加工処理された軟化魚肉または畜肉製品の製造方法において、
魚肉または畜肉素材をテンダライズ処理する工程と、
タンブリング処理または減圧処理により、テンダライズ処理された魚肉素材または畜肉素材内にタンパク質分解酵素を含む酵素液を浸透させる工程と、
魚肉素材または畜肉素材に浸透したタンパク質分解酵素を魚肉素材または畜肉素材に作用させる工程と、
を有することを特徴とする。
The method for producing a softened fish meat or livestock meat product according to the present invention is a method for producing a softened fish meat or livestock meat product subjected to a softening process,
A process of tenderizing fish or livestock meat,
A process of infiltrating an enzyme solution containing a proteolytic enzyme into a fish material or livestock material that has been subjected to a tumbling process or a decompression process;
A step of allowing a proteolytic enzyme that has penetrated into a fish material or a meat material to act on a fish material or a meat material;
It is characterized by having.

高齢人口は年々増加しており、2030年には約3人に1人が65歳以上と見込まれ、要介護者数も2013年には512万人にも上ると推測されている。素材そのままおいしさ,色,形状,栄養素を保持した介護食(咀嚼困難者向け製品)のニーズは高く、そのニーズに対応した食品の開発が可能となる。   The elderly population is increasing year by year, and about 1 in 3 people is expected to be over 65 years old in 2030, and the number of people requiring care is estimated to be 5.12 million in 2013. The needs of nursing foods (products for people with difficulty in chewing) that retain the taste, color, shape, and nutrients of the raw materials are high, and it is possible to develop foods that meet those needs.

本発明により、表面の過度の軟化や身割れなどによる形状崩壊を抑制し、見た目もおいしく、やわらかい軟化魚肉または畜肉製品が提供できる。そのやわらかさは、日本介護食品協議会が定めるユニバーサルデザインフードの区分2を満たす、歯ぐきでつぶせるやわらかさであり、咀嚼が困難な高齢者の方などが、満足感をもって摂取することができる軟化魚肉および畜肉製品を提供が可能となる。   According to the present invention, it is possible to provide a soft soft fish or livestock meat product that suppresses shape collapse due to excessive softening or cracking of the surface, has a good appearance, and is soft. The softness of the softened fish that satisfies the Category 2 of Universal Design Food as defined by the Japan Nursing Food Council and is soft enough to be crushed by the gums and can be consumed with satisfaction by elderly people who have difficulty chewing. And livestock products can be provided.

1.魚肉または畜肉素材
処理対象となる原料としての加工素材は、目的とする柔らかさを有する魚肉または畜肉製品用であれば魚肉や畜肉であればなんでもよく、基本的に生原料を使うが、解凍原料でも良い。すなわち、表面の過度の軟化や身割れなどによる形状崩壊を抑制し、見た目もおいしく、やわらかい軟化魚肉または畜肉製品、特に、日本介護食品協議会が定めるユニバーサルデザインフードの区分2を満たす、歯ぐきでつぶせるやわらかさであり、咀嚼が困難な高齢者の方などが、満足感をもって摂取することができる軟化魚肉および畜肉製品を得ることができるものであればよい。
1. Fish meat or livestock meat The raw material to be processed can be any fish meat or livestock meat that has the desired softness, and any raw fish is used. But it ’s okay. In other words, it suppresses shape collapse due to excessive softening or cracking of the surface, and it looks delicious and soft softened fish meat or meat products, in particular, meets Category 2 of Universal Design Foods established by the Japan Nursing Food Council, and can be crushed with gums What is necessary is just to be able to obtain softened fish meat and livestock products that are soft and can be consumed with satisfaction by elderly people who are difficult to chew.

加工素材の形状は特に制限されず、適宜加工処理された魚体全体、魚体から得た切り身やブロック、畜肉のブロックや切り身など、製品の用途に応じて適宜選択できる。必要に応じて皮などの部分を有していてもよい。   The shape of the processed material is not particularly limited, and can be appropriately selected according to the use of the product, such as the whole processed fish body, the fillet and block obtained from the fish body, and the livestock block and fillet. You may have parts, such as skin, as needed.

加工素材を得るための原料としては、サバ、サワラ、スケトウダラ、ホキ、イカなどを挙げることができる。更に、赤魚、シルバー、サケ、エビ、鶏肉、豚肉、牛肉などを挙げることができる。   Examples of the raw material for obtaining the processed material include mackerel, mackerel, walleye pollack, hoki and squid. Further examples include red fish, silver, salmon, shrimp, chicken, pork, and beef.

2.テンダライズ処理
本発明では、魚肉または畜肉素材に対してテンダライズ処理を行う。テンダライズ処理における穿孔密度は高いほど効果的な軟化処理が可能であり、1個/7.5mm×7.5mm以上の穿孔密度、すなわち、7.5mm×7.5mmの単位面積あたり一個以上の穿孔が形成されることが好ましい。このような穿孔密度は、1本/7.5mm×7.5mm以上の針密度での針の配置により達成することができる。
2. Tenderization process In the present invention, a tenderization process is performed on fish meat or livestock meat materials. The higher the perforation density in the tenderization process, the more effective the softening process is possible. The perforation density of 1 piece / 7.5 mm × 7.5 mm or more, that is, one or more perforations formed per unit area of 7.5 mm × 7.5 mm It is preferred that Such perforation density can be achieved by needle placement at a needle density of 1 / 7.5 mm × 7.5 mm or more.

テンダライズ処理は、先の尖った細い針状の器具を用いて行うことができ、例えば、ようじ、ドライバー、錐、千枚通し等を用いることができる。また、一度に複数の穴をあけるために、食器として用いられる家庭用フォークや生け花用の剣山、およびテンダライザー等も用い得る。さらに、カッター、ドリル、ミシン等を含む自動穴あけ機、ナイフなどの刃物類、レーザー、超音波、風圧および水圧等を利用した傷つけ処理も含む。テンダライズ処理は、次の工程の酵素処理の際に、魚肉や畜肉の内部に酵素が浸透するようにするために行う。   The tenderization process can be performed using a thin needle-like instrument having a sharp point, and for example, a toothpick, a driver, a cone, a threader, or the like can be used. Moreover, in order to open a plurality of holes at a time, a household fork used as tableware, a sword mountain for ikebana, a tenderizer, or the like can also be used. Further, it includes an automatic drilling machine including cutters, drills, sewing machines, etc., blades such as knives, scratching processes using laser, ultrasonic waves, wind pressure, water pressure, and the like. The tenderization process is performed so that the enzyme penetrates into the fish meat or livestock meat during the enzyme treatment in the next step.

3.酵素液浸透処理
テンダライズ処理された加工素材に対してタンパク質分解酵素を含有する酵素液を浸透させる。酵素液を浸透させる方法としては、減圧処理またはタンブリング処理が用いられる。
3. Enzyme solution permeation treatment An enzyme solution containing a proteolytic enzyme is permeated into the processed material subjected to the tenderization treatment. As a method for allowing the enzyme solution to permeate, decompression treatment or tumbling treatment is used.

3−1.酵素液
使用できるタンパク質分解酵素としては、例えばバシラス(Bacillus)属(例えばバシラス・サチリス(Bacillus subtilis)、バシラス・サーモプロテオティカス(Bacillus thermoproteolyticus)、バシラス・リシェニフォルミス(Bacillus licheniformis)等の産生する酵素、アスペルギルス(Aspergillus)属(例えばアスペルギルス・オリーゼ(Aspergillus oryzae)、アスペルギルス・ニガー(Aspergillus niger)、アルペルギルス・メレンス(Aspergillus mellens)等)の産生する酵素、リゾパス(Rhizopus)属(例えばリゾパス・ニベウス(Rhizopus niveus)、リゾパス・デレマー(Rhizopus delemar)等)の産生する酵素、ペプシン、パンクレアチン、パパイン、ブロメライン等が挙げられる。これらの酵素は単独、又は2種以上を組み合わせても良い。酵素は、対象の種類によっても異なるが、基質に対して、酵素0.01〜10重量%を添加し、よく懸濁して用いる。酵素とともに調味液等を添加しておいても良い。
3-1. Examples of proteolytic enzymes that can be used include production of the genus Bacillus (for example, Bacillus subtilis, Bacillus thermoproteolyticus, Bacillus licheniformis, etc.) Enzymes produced by the genus Aspergillus (eg Aspergillus oryzae, Aspergillus niger, Aspergillus mellens, etc.), Rhizopus genus (eg Rhizopus nibe) (Rhizopus niveus), Rhizopus delemar, etc.), pepsin, pancreatin, papain, bromelain, etc. These enzymes may be used alone or in combination of two or more. Depending on the type of target, Enzyme 0.01 to 10% by weight is added and suspended well, and a seasoning solution or the like may be added together with the enzyme.

3−2.減圧処理
減圧下で酵素液と魚肉または畜肉素材を接触させる減圧処理により、酵素液を魚肉または畜肉素材中に含ませることができる。酵素液を魚肉または畜肉素材に接触させる方法としては種々の方法を利用でき、例えば、減圧下での浸漬処理が利用できる。より詳細には上記の物理的処理を施したものを、上記の濃度で調製した酵素液に漬けて、好ましくは720mmHG以上にて5分間以上の減圧処理を実施する。酵素液の中に素材が浸る程度に酵素液を入れておくことが好ましい。なお、減圧処理により、素材により異なるが、素材重量の1%〜20%程度の酵素液が含浸される。
3-2. Decompression treatment The enzyme solution can be contained in the fish meat or livestock meat material by a decompression treatment in which the enzyme solution and the fish meat or livestock meat material are brought into contact under reduced pressure. Various methods can be used as the method for bringing the enzyme solution into contact with fish meat or livestock meat materials. For example, immersion treatment under reduced pressure can be used. More specifically, the material subjected to the above physical treatment is immersed in the enzyme solution prepared at the above concentration, and a reduced pressure treatment is preferably performed at 720 mmHG or more for 5 minutes or more. It is preferable to add the enzyme solution to such an extent that the material is immersed in the enzyme solution. It should be noted that, depending on the material, the reduced pressure treatment is impregnated with an enzyme solution of about 1% to 20% of the material weight.

3−3.タンブリング処理(酵素液を機械的に浸透する処理)
上記3−2の減圧処理の代わりにタンブリング処理でも良い。タンブリング処理の場合、長時間の方が、加工素材は効果的に軟化するが、処理時間が長すぎても身割れが起きる場合がある。加工素材の種類や目的とする製品の用途などに応じてタンブリング処理時間を、例えば、10分以上の処理時間から適宜設定することができる。
3-3. Tumbling treatment (treatment that mechanically permeates the enzyme solution)
A tumbling process may be used instead of the decompression process of 3-2. In the case of the tumbling treatment, the processed material is effectively softened for a long time, but the body may be cracked even if the treatment time is too long. The tumbling processing time can be appropriately set from, for example, a processing time of 10 minutes or more according to the type of the processed material and the intended product application.

タンブリング処理により、畜肉又は魚肉加工素材中で、酵素液を均一に分散、浸透させることが可能になる。かかるタンブリング処理は、畜肉加工用として一般的に使用されている回転ドラム(タンブリングマッサージ機)を使用し、常圧下でも減圧下でもよい。減圧条件は、用いる素材等に応じて選択できる。なお、タンブリング処理により、素材により異なるが、素材重量の0.1%〜35%程度の酵素液が含浸される。   By the tumbling treatment, it becomes possible to uniformly disperse and infiltrate the enzyme solution in the raw meat or fish processed material. The tumbling process uses a rotating drum (tumbling massage machine) generally used for processing livestock meat, and may be under normal pressure or under reduced pressure. The decompression condition can be selected according to the material used. The tumbling treatment impregnates an enzyme solution of about 0.1% to 35% of the material weight, although it varies depending on the material.

4.酵素反応条件
加工素材中に浸透した酵素液に含まれるタンパク質分解酵素によるタンパク質分解反応を行わせる。タンパク質分解反応の条件は、目的を達成できる条件であればよく、反応温度としては至適pHが好ましいが、低温(4℃程度)から高温(60℃程度)での反応でも良い。タンパク質分解反応のための処理時間は、用いる酵素の種類や目的とする製品に要求される物性等に応じて選択できる。例えば、酵素の至適温度付近では数分〜数時間程度、至適温度以下の低温での反応では、数時間〜数日の処理とすることができる。
4). Enzymatic reaction conditions Proteolytic reaction by proteolytic enzymes contained in the enzyme solution that has penetrated into the processed material is performed. The conditions for the proteolytic reaction may be any conditions that can achieve the object, and the optimum reaction temperature is preferably an optimum pH, but may be a reaction at a low temperature (about 4 ° C.) to a high temperature (about 60 ° C.). The processing time for the proteolytic reaction can be selected according to the type of enzyme used and the physical properties required for the target product. For example, the reaction can be performed for several minutes to several hours in the vicinity of the optimum temperature of the enzyme and for several hours to several days in a reaction at a low temperature below the optimum temperature.

(実施例1、比較例1及び2)物理的処理(テンダライズ)と減圧処理との併用効果
<対象>ホキ、赤魚、スケソウダラ、サバ、サワラ、サケ(いずれも凍結原料)
<処理>
以下の処理工程から表1に示す工程の組合せを選択して加工素材を処理した。
(Example 1, Comparative Examples 1 and 2) Combined effects of physical treatment (tenderization) and reduced pressure treatment <Target> Hoki, red fish, walleye pollack, mackerel, sawara, salmon (all frozen ingredients)
<Processing>
Processing materials were processed by selecting a combination of steps shown in Table 1 from the following processing steps.

原料解凍→物理的処理(1)→減圧処理(2)→酵素反応(3)→加熱失活(90℃、5分)→急速冷凍
(1)物理的処理
(1−1)テンダライズ(針による穴あけ):針密度7.5mm×7.5mm。テンダライズ後、酵素液に15分間浸漬した後、減圧処理を行う。
(1−2)酵素液インジェクション:ピュコマット社製 SP20/300型 インジェクターを使用。針密度1本/7.5mm×7.5mm。注入条件は0.1MPa×0.4秒。
(2)減圧処理
酵素液を注入した素材を、真空ポンプを用いて、720mmHg下で20分間の減圧下で処理した。
(3)酵素:プロテックス7L(ジェネンコア協和)、酵素濃度:0.5重量%、酵素反応:50℃, 3時間
<物性測定>
テクスチャーアナライザー TA-XTplus(Satable Micro Systems社製)により、軟化度を数値化した。プランジャーは直径20mmの円柱型を用い、進入速度10mm/secで試料を70%まで潰したときの荷重(N)から応力(N/m2)を算出した(缶詰時報, Vol.90, No.1, 71-73(2011))。なお、応力50,000N/m2以下が、日本介護食品協議会が定めるユニバーサルデザインフードの区分2に相当する。
<試験区>
Raw material thawing → Physical treatment (1) → Decompression treatment (2) → Enzymatic reaction (3) → Heat inactivation (90 ° C, 5 minutes) → Quick freezing (1) Physical treatment (1-1) Tenderization (by needle Drilling): Needle density 7.5mm x 7.5mm. After tenderization, the sample is immersed in an enzyme solution for 15 minutes and then subjected to reduced pressure treatment.
(1-2) Enzyme solution injection: A SP20 / 300 type injector manufactured by Pureco mat is used. Needle density is 1 / 7.5mm × 7.5mm. Injection conditions are 0.1 MPa x 0.4 seconds.
(2) Decompression treatment The material into which the enzyme solution was injected was treated under reduced pressure for 20 minutes under 720 mmHg using a vacuum pump.
(3) Enzyme: Protex 7L (Genencor Kyowa), Enzyme concentration: 0.5 wt%, Enzyme reaction: 50 ° C, 3 hours <Physical property measurement>
The degree of softening was quantified using a texture analyzer TA-XTplus (manufactured by Satable Micro Systems). The plunger was a cylinder with a diameter of 20 mm, and the stress (N / m 2 ) was calculated from the load (N) when the sample was crushed to 70% at an entry speed of 10 mm / sec (canned time signal, Vol. 90, No. .1, 71-73 (2011)). A stress of 50,000 N / m 2 or less corresponds to the category 2 of universal design food established by the Japan Nursing Food Council.
<Test area>

Figure 2011092216
Figure 2011092216

<結果> <Result>

Figure 2011092216
Figure 2011092216

Figure 2011092216
Figure 2011092216

(実施例2)調味液入り酵素液での反応
<対象>サバ
<処理>
以下の処理工程により加工素材を処理した。
(Example 2) Reaction with enzyme solution containing seasoning liquid <Target> Mackerel <Treatment>
The processed material was processed by the following processing steps.

原料解凍→テンダライズ処理(1)→減圧処理(2)→酵素反応(3)→加熱失活(90℃、5分)→急速冷凍
(1)テンダライズ処理:針密度1本/7.5mm×7.5mm。テンダライズ後、酵素液に15分間浸漬した後、減圧処理を行う。
(2)減圧処理
真空ポンプを用いて、720mmHg下で20分間の減圧下で処理した。
(3)酵素:プロテックス7L(ジェネンコア協和)、酵素濃度:0.5%、酵素反応:50℃, 3時間
※調味液入り試験区では、酵素:プロテックス7L:0.5%、砂糖:3.75%、並塩:1.5%、濃口醤油:0.75%, グルタミン酸ナトリウム:0.3%の組成の調味液を用いた。
<結果>
Raw material thawing → Tenderization treatment (1) → Depressurization treatment (2) → Enzyme reaction (3) → Heat inactivation (90 ° C, 5 minutes) → Quick freeze (1) Tenderization treatment: Needle density 1 / 7.5mm x 7.5mm . After tenderization, the sample is immersed in an enzyme solution for 15 minutes and then subjected to reduced pressure treatment.
(2) Decompression treatment Using a vacuum pump, the treatment was conducted under a reduced pressure of 720 mmHg for 20 minutes.
(3) Enzyme: Protex 7L (Genencore Kyowa), Enzyme concentration: 0.5%, Enzyme reaction: 50 ° C, 3 hours * In the test zone with seasoning liquid, enzyme: Protex 7L: 0.5%, sugar: 3.75%, common salt: A seasoning liquid having a composition of 1.5%, concentrated soy sauce: 0.75%, and sodium glutamate: 0.3% was used.
<Result>

Figure 2011092216
Figure 2011092216

(実施例3、比較例3及び4)物理的処理(テンダライズ)とタンブリング処理との併用効果
<対象>鶏肉、豚肉、イカ、エビ(いずれも凍結原料)
<処理>
以下の処理工程から表4に示す工程の組合せを選択して加工素材を処理した。
(Example 3, Comparative Examples 3 and 4) Combined effects of physical treatment (tenderization) and tumbling treatment <Target> Chicken, pork, squid, shrimp (all frozen ingredients)
<Processing>
Processing materials were processed by selecting a combination of steps shown in Table 4 from the following processing steps.

原料解凍→テンダライズ処理(1)→タンブラー処理(2)→酵素反応(3)→加熱失活(90℃、5分)→急速冷凍
(1)テンダライズ処理:針密度1本/7.5mm×7.5mm。テンダライズ後、酵素液に15分間浸漬した後、タンブラー処理を行う。
(2)タンブラー処理
真空撹拌タンク装置にて行った(21回転/分×15分)。
(3)酵素:ブロメラインF(アマノエンザイム)、酵素濃度:0.1%、酵素反応:50℃3時間
<試験区>
Raw material thawing → Tenderization treatment (1) → Tumbler treatment (2) → Enzymatic reaction (3) → Heat inactivation (90 ° C, 5 minutes) → Quick freezing (1) Tenderization treatment: needle density 1 / 7.5mm x 7.5mm . After tenderization, immerse in an enzyme solution for 15 minutes, and then perform tumbler treatment.
(2) Tumbler treatment It was carried out in a vacuum stirring tank device (21 rotations / minute × 15 minutes).
(3) Enzyme: Bromelain F (Amanoenzyme), Enzyme concentration: 0.1%, Enzyme reaction: 50 ° C for 3 hours <Test section>

Figure 2011092216
Figure 2011092216

<結果>
テンダライズ処理とタンブラー処理を併用した試験区(F)のみ、物性値が50,000N/m2以下となった。
<Result>
Only in the test section (F) where the tenderization treatment and the tumbler treatment were used in combination, the physical property value was 50,000 N / m 2 or less.

Figure 2011092216
Figure 2011092216

(実施例4)物理的処理(テンダライズ)とタンブリング処理との併用効果(酵素濃度の影響)
<対象>イカ
<処理>
以下の処理工程により加工素材を処理した。
(Example 4) Combined effect of physical treatment (tenderization) and tumbling treatment (effect of enzyme concentration)
<Target> Squid <Processing>
The processed material was processed by the following processing steps.

原料解凍→テンダライズ処理(1)→タンブラー処理(2)→酵素反応(3)→加熱失活(90℃、5分)→急速冷凍
(1)テンダライズ処理:針密度1本/7.5mm×7.5mm。テンダライズ後、酵素液に15分間浸漬した後、タンブラー処理を行う。
(2)タンブラー処理
真空撹拌タンク装置にて行った(21回転/分×15分)。
(3)酵素:ブロメラインF(アマノエンザイム)、酵素濃度:0.1〜1.0%、酵素反応:50℃3時間
<物性測定>
テクスチャーアナライザー TA-XTplus(Satable Micro Systems社製)により、軟化度を数値化した。プランジャーは直径20mmの円柱型を用い、進入速度10mm/secで試料を70%まで潰したときの荷重(N)から応力(N/m2)を算出した(缶詰時報, Vol.90, No.1, 71-73(2011))。
日本介護食品協議会が定めるユニバーサルデザインフード(UDF)の区分1は応力500,000 N/m2以下、区分2は応力50,000N/m2以下、区分3は20,000 N/m2以下に相当する。
<結果>
Raw material thawing → Tenderization treatment (1) → Tumbler treatment (2) → Enzymatic reaction (3) → Heat inactivation (90 ° C, 5 minutes) → Quick freezing (1) Tenderization treatment: needle density 1 / 7.5mm x 7.5mm . After tenderization, immerse in an enzyme solution for 15 minutes, and then perform tumbler treatment.
(2) Tumbler treatment It was carried out in a vacuum stirring tank device (21 rotations / minute × 15 minutes).
(3) Enzyme: Bromelain F (Amanoenzyme), Enzyme concentration: 0.1-1.0%, Enzyme reaction: 50 ° C for 3 hours <Physical property measurement>
The degree of softening was quantified using a texture analyzer TA-XTplus (manufactured by Satable Micro Systems). The plunger was a cylinder with a diameter of 20 mm, and the stress (N / m 2 ) was calculated from the load (N) when the sample was crushed to 70% at an entry speed of 10 mm / sec (canned time signal, Vol. 90, No. .1, 71-73 (2011)).
Japan Care Category 1 of universal design hood (UDF) the food council stipulated the stress 500,000 N / m 2 or less, Category 2 is stress 50,000N / m 2 or less, Category 3 is equivalent to 20,000 N / m 2 or less.
<Result>

Figure 2011092216
Figure 2011092216

本願発明にかかる製造方法によれば、日本介護食品協議会が定めるユニバーサルデザインフードの区分2(物性規格は硬さ上限値が5×104 N/m2)を満たすやわらかさの軟化魚肉または畜肉製品を提供することが可能となる。 According to the manufacturing method according to the invention of the present application, soft soft fish meat or livestock meat that satisfies Category 2 of the universal design food defined by the Japan Nursing Food Council (the physical property standard is a hardness upper limit of 5 × 10 4 N / m 2 ). Products can be provided.

また、酵素の種類、酵素濃度、減圧条件、酵素反応条件(温度・時間)など調節することで、区分1(物性規格は硬さ上限値5×105 N/m2)から区分3(物性規格は硬さ上限値2×104 N/m2)までの任意の硬さに調整することも可能となる。 In addition, by adjusting the enzyme type, enzyme concentration, reduced pressure conditions, enzyme reaction conditions (temperature / time), etc., Category 1 (physical property standard is hardness upper limit 5 × 10 5 N / m 2 ) to Category 3 (physical properties) The standard can be adjusted to any hardness up to a hardness upper limit of 2 × 10 4 N / m 2 ).

Claims (10)

軟化加工処理された軟化魚肉または畜肉製品の製造方法において、
魚肉または畜肉素材をテンダライズ処理する工程と、
タンブリング処理または減圧処理により、テンダライズ処理された魚肉素材または畜肉素材内にタンパク質分解酵素を含む酵素液を浸透させる工程と、
魚肉素材または畜肉素材に浸透したタンパク質分解酵素を魚肉素材または畜肉素材に作用させる工程と、
を有することを特徴とする軟化魚肉または畜肉製品の製造方法。
In the method for producing softened fish meat or livestock meat products that have been softened,
A process of tenderizing fish or livestock meat,
A process of infiltrating an enzyme solution containing a proteolytic enzyme into a fish material or livestock material that has been subjected to a tumbling process or a decompression process;
A step of allowing a proteolytic enzyme that has penetrated into a fish material or a meat material to act on a fish material or a meat material;
A method for producing a softened fish meat or livestock meat product, comprising:
テンダライズ処理における針密度が、1本/7.5mm×7.5mm以上である請求項1に記載の製造方法。   The manufacturing method according to claim 1, wherein the needle density in the tenderization treatment is 1 / 7.5 mm × 7.5 mm or more. 酵素液が、0.01〜10重量%のタンパク質分解酵素を含有する請求項1または2に記載の製造方法。   The production method according to claim 1 or 2, wherein the enzyme solution contains 0.01 to 10% by weight of a proteolytic enzyme. 減圧処理を、720mmHG以上、5分間以上の条件で行なう請求項1〜3のいずれか1項に記載の製造方法。   The manufacturing method of any one of Claims 1-3 which perform a pressure reduction process on the conditions for 720 mmHG or more and 5 minutes or more. タンブリング処理を減圧下で行う請求項1〜3のいずれか1項に記載の製造方法。   The manufacturing method of any one of Claims 1-3 which perform a tumbling process under reduced pressure. タンパク質分解酵素を作用させる工程を4℃〜60℃の温度で行なう請求項1〜5のいずれか1項に記載の製造方法。   The production method according to any one of claims 1 to 5, wherein the step of causing the protease to act is performed at a temperature of 4 ° C to 60 ° C. タンパク質分解酵素を作用させた魚肉素材または畜肉素材の硬さが、5×105N/m2以下である請求項1〜6のいずれか1項に記載の製造方法。 The manufacturing method according to any one of claims 1 to 6, wherein the hardness of the fish meat material or livestock meat material on which the proteolytic enzyme is allowed to act is 5 x 10 5 N / m 2 or less. タンパク質分解酵素を作用させた魚肉素材または畜肉素材の硬さが、5×104N/m2以下である請求項1〜6のいずれか1項に記載の製造方法。 The manufacturing method according to any one of claims 1 to 6, wherein the hardness of the fish meat material or livestock meat material on which a proteolytic enzyme is allowed to act is 5 x 10 4 N / m 2 or less. タンパク質分解酵素を作用させた魚肉素材または畜肉素材の硬さが、2×104N/m2以下である請求項1〜6のいずれか1項に記載の製造方法。 The manufacturing method according to any one of claims 1 to 6, wherein the hardness of the fish meat material or livestock meat material on which the proteolytic enzyme is allowed to act is 2 x 10 4 N / m 2 or less. 請求項1〜9のいずれか1項に記載の製造方法により製造された軟化魚肉または畜肉製品。   The softened fish meat or livestock meat product manufactured by the manufacturing method of any one of Claims 1-9.
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