JP2011067180A - Food and drink composition, and medicinal composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a food and drink composition having fig (Ficus carica L.) as a raw material, and free from fear of an onset of skin disease dermatosis by light, such as phototoxic contact dermatitis, and an increase in side effect of medicine, and to provide a medicinal composition. <P>SOLUTION: The food and drink composition includes as the raw material a fig variety containing no furocoumarin and glycoside related to furocoumarin. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a edible composition and a medicinal composition made from figs.

  Figs are mulberry plants originating in southern Arabia and have been used not only for eating and drinking but also for medicinal plants for a long time (see Non-Patent Document 1). In recent studies, it has also been clarified that figs have a blood glucose level lowering action, a blood pressure lowering action, an anticancer action, an action to enhance immunity, and the like (see Non-Patent Document 2).

Ray Tanahill, Translated by Setsuko Kuriyama, “The Fine Art of Food”, Tokyo, Yasaka Shobo, November 25, 2008, p12-18 By Chizuru Liang, “Toho Nutrition Shinsho”, Kyoto, Medical Yukon, 2005, p. 70-71

  Although it is a fig useful for eating and drinking and medicinal purposes, it contains furocoumarins as an ingredient. These furocoumarins may cause phototoxic contact dermatitis that causes symptoms of erythema, pigmentation, and erosion by ingesting or adhering to the skin, or side effects of the drug may increase and cause health damage. . This is thought to be due to the fact that furocoumarins act as photosensitizers or inhibit the action of certain drug-metabolizing enzymes. In fact, there have been reports of cases in which phototoxic contact dermatitis with the above-mentioned symptoms was caused by running water with water that squeezed fig leaves (Tamoru Tani, “Skin damage caused by chemicals 36 Phototoxicity caused by plants” Contact Dermatitis ", Med. Journal 38, 2002, p 2398-2405).

  Accordingly, an object of the present invention is to provide a food-drinking composition and a medicinal composition using fig as a raw material, without causing the onset of skin diseases caused by light such as phototoxic contact dermatitis and the side effects of drugs. is there.

  In order to achieve the above object, the first feature of the food and beverage composition and medicinal composition of the present invention is that the raw material is a fig variety that does not contain furocoumarins and furocoumarin-related glycosides.

[Action and effect]
The food-drinking composition and medicinal composition of the present invention are configured using fig varieties that do not contain furocoumarins and furocoumarin-related glycosides that can be hydrolyzed to produce furocoumarins. For this reason, even if the eating and drinking composition and the medicinal composition of the present invention are ingested, there is no risk of causing health damage such as the onset of skin diseases caused by light such as phototoxic contact dermatitis and the increase of drug side effects. .

  The 2nd characteristic structure which concerns on the food-drinks composition and medicinal composition of this invention exists in the point whose said fig varieties are a grease de tarascon (Grise de Talascon) or a biore dauphin (Violet Dauphine).

[Action and effect]
As shown in Examples described later, the two varieties of Grease de Tarascon and Biore Dauphin are at least psoralen and bergapten of furocoumarins, and isopsolar acid glucoside (hereinafter referred to as IPG) of furocoumarin-related glycosides. Further, it has been confirmed by the present inventors that the compound does not contain a compound in which one methoxyl group is added to isopsolar acid glucoside (hereinafter referred to as mIPG).
In addition, it is thought that psoralen and bergapten are produced | generated by each hydrolyzing IPG and mIPG.
That is, the two types of grease de tarascon and biore dofin do not contain psoralen and bergapten, and since IPG and mIPG are not contained, there is no possibility that psoralen and bergapten are produced.
Therefore, the composition for food and drink and the medicinal composition of the present invention have no risk of developing a skin disease caused by light such as phototoxic contact dermatitis, which is particularly associated with psoralen, bergapten, etc. Can also be taken with peace of mind.

It is a HPLC chromatogram of the aqueous extract of Precos Ronde de Bordeaux species. It is a HPLC chromatogram of a methanol extract of 0.1% hydrochloric acid added by Precos Ronde de Bold type. It is a HPLC chromatogram of the water extract of Sakurai Dauphin seeds. It is a HPLC chromatogram of a water extract of Xenopus. It is a HPLC chromatogram of the water extract of a grease de tarascon species. It is a HPLC chromatogram of the aqueous extract of Biore Dauphin species. It is a liquid chromatogram in LC-MS of 0.1% hydrochloric acid addition methanol extract of Sakurai Dauphin seed | species. It is a liquid chromatogram in LC-MS of a 0.1% hydrochloric acid addition methanol extract of a certain kind. It is a liquid chromatogram in LC-MS of a 0.1% hydrochloric acid addition methanol extract of Grease de Tarascon species. It is a liquid chromatogram in LC-MS of a 0.1% hydrochloric acid addition methanol extract of Biore Dauphin species. It is a mass spectrum of the peak (elution time 29.913 minutes) containing IPG in LC-MS of 0.1% hydrochloric acid addition methanol extract of Sakurai Dauphin seeds. It is a mass spectrum of an elution time of 29.808 minutes in LC-MS of a 0.1% hydrochloric acid-added methanol extract of Grease de Tarascon species. It is a mass spectrum of 30.173 minutes of elution time in LC-MS of a 0.1% hydrochloric acid addition methanol extract of Biore Dauphin species.

Terms used in this specification will be described.
(FIG)
Fig (Ficus carica L.) applied to the present invention is a plant of the mulberry family that originates in southern Arabia.

  The fig variety applicable to the present invention is not particularly limited as long as it is a fig variety that does not contain furocoumarins and furocoumarin-related glycosides. For example, “Grise de Tarascon” Or “Violet Dauphine”.

  As used herein, the term “ficus varieties that do not contain furocoumarins and furocoumarin-related glycosides” literally includes not only varieties that do not contain these compounds at all, but also varieties that hardly contain these compounds. .

  The varieties that do not contain furocoumarins and furocoumarin-related glycosides are a specified solvent (water or a mixture of water and acetone, or methanol (about 0.1% hydrochloric acid) for its fruits, leaves, stems, roots, etc. ) Means a variety in which these compounds were not detected even after extraction and analysis.

  The varieties that hardly contain furocoumarins and furocoumarin-related glycosides have a weight (mg) of furocoumarins per gram of dry matter of 0.1 mg or less, and the weight (mg) of furocoumarin-related glycosides of 0.1 mg. It means the following varieties.

(Furocoumarins)
Furocoumarins is a general term for coumarin derivatives condensed with a furan ring. Furocoumarins are abundant in plants such as celery family, citrus family, legume family, mulberry family, asteraceae family, and hypericum family. Examples of the compounds belonging to furocoumarins include psoralen, bergapten, xanthotoxin, isopimpinellin, bergamotin, dihydroxybergamotine, etc.

  Furocoumarins have antibacterial action and action to prevent digestion of insects that eat plants, and are considered to be one of the substances responsible for the defense mechanism of plants. May cause some health hazards in the affected human.

  For example, exposure to sunlight (ultraviolet rays) with psoralen attached to the skin may increase the degree of sunburn, cause spots, and even phototoxic contact dermatitis (Mr. Tani, “ Skin damage caused by chemical substances 36 Phototoxic contact dermatitis caused by plants ", Medicinal Journal, 2002, 38, p2398-2405). This is thought to be because furocoumarins have a so-called photosensitizing action that promotes the reaction and fermentation process by passing energy obtained by absorbing light to other substances. .

  Further, when a certain drug is taken together with bergamotine or dihydroxybergamotin, side effects of the drug may be increased. This is considered to be because bergamotine and dihydroxybergamotin inhibit the function of a drug-metabolizing enzyme (CYP3A4) present in small intestinal epithelial cells. CYP3A4 has a function of reducing the amount of a drug transferred to the circulating blood by metabolizing a certain drug to some extent and inactivating it. Therefore, when the function of CYP3A4 is inhibited by bergamotin or dihydroxybergamotin, the inactivation of the drug by CYP3A4 is prevented, resulting in an increase in the amount of drug that moves into the circulating blood, resulting in the drug being too effective and causing side effects It will increase.

  In addition, regarding tolerable doses when orally ingesting furocoumarins, it is not always clear about all furocoumarins, but according to a study that examined the tolerable amount of bergapten and xanthotoxin in humans, There is a possibility that phototoxicity may occur if 15 mg or more is ingested and exposed to ultraviolet rays, and if it is 10 mg or less, the possibility of developing is low (Schlatter, J., Zimmerli, B., Dick, R). , Panizzon, R. and Schlatter, Ch., “Dietary Intake and Risk Assessment of Phototoxicology in Humans”, Food Chem. Toxicology, 1991, 29, 295, 295, pp. ).

(Furocoumarin-related glycosides)
The furocoumarin-related glycoside in the present invention means a substance that generates furocoumarins upon hydrolysis. For example, isopsolar acid glucoside (hereinafter referred to as IPG), and isopsolar acid glucoside has a methoxyl group. The compound shown in the following [Chemical Formula 1] (hereinafter referred to as mIPG) and the like are included (Naoki Kasajima, Takahito Furutake, Sumiko Shioda, Yuuki Kaneda, “The 55th Annual Meeting of the Japanese Biopharmaceutical Society” Abstracts of Nagasaki 2008 (Studies on Medicinal Plant Resources, Components of Leaves of Ficus carica (First Report)), Japan Biopharmaceutical Society, September 19-20, 2008, p238.

  IPG is also contained in the seeds of leguminous plants, Psolalea corifolia L., which is a raw material for the Chinese herbal medicine “prosthetic fat” and generates psoralen when hydrolyzed (Qiao, C.-). F., Han, Q.-B., Mo, S.-F., Song, J.-Z., Xu, L.-J., Chen, S.-L., Yang, D.-J. , Kong, L.-D., Kung, H.-F. and Xu, H.-X., “Psoralenoid and Isosoralenoside, Two New Benzofuran Glycosides from Psoralea Coli. P714-716). Moreover, when mIPG is hydrolyzed, it is estimated that bergapten is produced.

Embodiment
The present invention uses, as a raw material, fruits, leaves, stems, roots, etc. of fig varieties (eg, grease de tarascon or biore dofin) that do not contain furocoumarins and furocoumarin-related glycosides, or extracts thereof. The present invention relates to a food and drink composition and a medicinal composition.

(Eating and drinking composition)
Examples of the composition for eating and drinking according to the present invention include tea leaves. The tea leaves are (1) a steaming process in which steam is heated in a short time by steaming the leaves of the above-described fig cultivar, (2) a cooling process in which the surface of the tea leaves is cooled while removing moisture, and (3) crude soft Put into the machine and dry with hot air and dry, (4) Applying force to the leaves and twisting while keeping moisture even, (5) Applying heat and force to the leaves and drying while adjusting the shape (6) A drying process in which the leaves are spread thinly and dried by applying hot air. (7) A sorting process in which the crude tea is separated or cut to prepare a shape. (8) The leaves are further dried. It can be produced by a known tea-making method comprising a drying step that draws out the unique aroma and taste, and (9) a packaging step that measures the finished leaves and packs them into a tea box or bag.
Examples of other food and beverage compositions include tea beverages, green juice, food ingredients for cooking, fruit wine, etc., and these food and beverage compositions are also produced by the known production methods using the above-described fig varieties as raw materials. Can do.

(Medicinal composition)
Examples of the medicinal composition according to the present invention include herbal medicines and extracts thereof. The herbal medicine can be produced by a known production method by drying the fruit or leaf of the above-described fig variety and cutting or crushing it into small pieces or small chunks or crushing it into powder.

  The herbal medicine extract refers to a liquid leached by adding a suitable leaching agent to the herbal medicine, or a liquid obtained by concentrating the leaching liquid, and specifically includes "extract" and "tincture". Examples of the leaching agent include water, ethanol, or a mixed solution of water and ethanol. In addition, the manufacturing method of an extract may use a well-known method (For example, the method of Japanese Pharmacopoeia).

  In addition, a product obtained by drying the “extract” is also included in the above extract, which is generally referred to as “dry extract”. A known method may be used as a method for producing the dried extract. In general, the same effect can be obtained in any form as long as the crude drug has the same base.

  The medicinal composition according to the present invention can be administered orally or parenterally, but is preferably administered orally. The dosage form of the preparation for oral administration (oral administration preparation) includes liquids and capsules such as extract, elixir, syrup, tincture, and limonade, granules, pills, powders, tablets, etc. Can be mentioned. The preparation containing the medicinal composition according to the present invention can be formulated into various dosage forms by known preparation techniques, and appropriate preparation additives can be added to the preparation. Specific examples of formulation additives include excipients, binders, disintegrants, suspending agents, preservatives, antioxidants, and corrigents. The formulation additive must be harmless to a living body such as a human body in the dosage of the formulation, and must not interfere with the effect of the active ingredient.

  For example, examples of the excipient include lactose, corn starch, and crystalline cellulose. Examples of the binder include gum arabic powder, dextrin, carboxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol and the like. Examples of the disintegrant include corn starch, carboxymethyl cellulose, carboxymethyl cellulose calcium, and crospovidone. Examples of the suspending agent include sodium alginate and polyvinyl pyrrolidone. Examples of the preservative include ethyl paraoxybenzoate and butyl paraoxybenzoate. Examples of the antioxidant include ascorbic acid and tocopherol. Examples of the corrigent include sucrose, honey, aspartame, stevia, and dipotassium glycyrrhizinate.

[Other Embodiments]
In addition to the above-mentioned eating and drinking composition and medicinal composition, as a composition that can be produced using fig varieties that do not contain furocoumarins and furocoumarin-related glycosides as raw materials, for example, a cosmetic composition, a composition for bathing agents, or Examples thereof include a dyeing composition for dyeing fibers.

[Examination of extraction solvent]
The extraction solvents for extracting furocoumarins and furocoumarin-related glycosides from fig leaves were investigated.
(experimental method)
1. Collection of leaves (1) Leaves were collected from the fig tree of the “Precos Ronde de Bordeaux” species.
(2) The collected leaves were cut into approximately 2 cm squares and immediately stored frozen.

2-1. First Extraction Method (1) Nine times the amount (weight ratio) of water was added to the frozen leaves and crushed with a mixer.
(2) The suspension was filtered with gauze and then centrifuged (500 g × 5 minutes) to obtain a supernatant.
(3) The sample was appropriately diluted and filtered through a membrane filter having a pore size of 0.45 μm to obtain a sample for analysis.

2-2. Second Extraction Method (1) Lyophilized leaves were pulverized, 0.2 g thereof was placed in an Erlenmeyer flask, and 30 mL of extraction solvent was added. The extraction solvent used was a mixture of 3 parts by weight of organic solvents (methanol, ethanol, propanol, acetone) with 1 part by weight of water.
(2) Extraction was performed while shaking with a circular motion of 120 revolutions per minute for 3 hours at room temperature.
(3) Centrifugation was performed at 12,000 rpm for 10 minutes, and the supernatant was collected.
(4) 10 mL of the extraction solvent was added to the residue and stirred, and centrifuged again at 12,000 rpm for 10 minutes to recover the supernatant.
(5) The above operation 4 was repeated once more.
(6) The collected supernatants were combined and made up to a volume of 50 mL.
(7) The sample for analysis was obtained by appropriately diluting with water and filtering through a membrane filter having a pore size of 0.45 μm.

2-3. 3rd extraction method It carried out by the method similar to the said 2nd extraction method. However, to the extraction solvent, 1 part by weight of methanol mixed with 1 part by weight of water, 3 parts by weight of methanol mixed with 1 part by weight of water, methanol and 0.1% hydrochloric acid were added. Methanol was used.

3. Analysis Method (1) 10 μL (microliter) of each analytical sample obtained by the above first to third extraction methods was injected into a high performance liquid chromatography (HPLC) apparatus for detection and quantification.
(2) HPLC analysis conditions were as follows.
(A) Column: Phenomenex AQUA C18, particle diameter 5 μm, inner diameter 4.6 mm × length 250 mm
(B) Mobile phase A: 2% acetic acid (c) Mobile phase B: 0.5% acetic acid and acetonitrile mixed at a ratio of 1: 1 (d) Mobile phase flow rate: 1 mL per minute
(E) Mobile phase gradient: The ratio of mobile phase B was changed as follows according to the analysis time. Analysis start time: 10%, 20 minutes later: 24%, 40 minutes later: 30%, 60 minutes later: 55%, 75 minutes later: 100%, until 83 minutes later: 100%, 85 minutes later: 10%, Until 90 minutes (end of analysis): 10%.
(F) Column temperature: 30 ° C
(G) Detection of component: Absorbance at a wavelength of 250 nm was measured with an absorbance detector.
(H) Elution time of each component: IPG: Detected as a peak at around 29-30 minutes, mIPG: around 31 minutes, psoralen: around 69-70 minutes, bergapten: around 75 minutes.

(Experimental result)
The HPLC chromatogram of the aqueous extract of fig leaf obtained by the first extraction method is shown in FIG. 1, and the peak areas of each component of psoralen, bergapten, IPG, and mIPG are shown in Table 1 below.

  As shown in FIG. 1 and Table 1, when water is used as the extraction solvent, psoralen and bergapten are extracted, but it is understood that IPG and mIPG are hardly extracted. The reason why IPG and mIPG were hardly contained in the water extract was that (a) the extraction efficiency of the component was extremely low, or (b) the enzyme maintained its activity and was decomposed by it. Conceivable.

  Four analytical samples obtained by the second extraction method, ie, fig leaf extract (water + methanol) in a mixture of 1 part by weight of water and 3 parts by weight of methanol, 1 part by weight of water and 3 parts by weight of ethanol Fig leaf extract (water + ethanol) in a mixed solution with Fig, fig leaf extract (water + propanol) in a mixture of 1 part by weight of water and 3 parts by weight of propanol, 1 part by weight of water and 3 parts by weight of acetone Table 2 below shows the peak areas of each component of psoralen, bergapten, IPG, and mIPG in the extract of fig leaf (water + acetone) by the mixed solution with the part. As shown in Table 2, IPG and mIPG were hardly extracted with any extraction solvent.

  Four analytical samples obtained by the third extraction method, that is, fig leaf extract (1 part by weight of water + 1 part by weight of methanol) and 1 part by weight of water using a mixture of 1 part by weight of water and 1 part by weight of methanol. Fig leaf extract (1 part by weight water + 3 parts by weight methanol), fig leaf extract (methanol) with methanol and 3 parts by weight methanol, fig leaf extract with methanol with 0.1% hydrochloric acid Table 3 below shows the peak areas of each component of psoralen, bergapten, IPG, and mIPG in the extract (0.1% hydrochloric acid-added methanol). In addition, the chromatogram of the sample for analysis regarding a 0.1% hydrochloric acid addition methanol extract is shown in FIG.

  As shown in Table 3, in the solvent containing water, the higher the methanol ratio, the better the extraction of psoralen and bergapten, but almost no IPG and mIPG were extracted. At 100% methanol, this relationship was reversed and IPG and mIPG were extracted very well, but the extraction of psoralen and bergapten was drastically reduced. When a small amount of hydrochloric acid was added to 100% methanol, the extraction of IPG and mIPG was slightly improved.

  From the above, extraction tests were conducted on various fig varieties using water and methanol with 0.1% hydrochloric acid as the extraction solvent.

[Extraction and analysis of fig leaf with water]
40 varieties of figs ("Sakurai Dauphin", "Sakai", "Nouard de Karon", "White Ischia", "Negrone", "Brunswick", "Burjasotto Gris", "Temari Figs", "Temari Figs" Portogaro, Biore Solies, Brown Turkey, Sugar, Artena, Celeste, White Zenoa, Pau Dur, California Black, Fig de Do Marseille, Cadota, Negro Lago, Precos Ronde de Bordeaux, Grease St Jean, Greez Beer, Aida, Mission, Grist Jean , "Gut Dole", "Bellone", "Early Dorfine", "Ronde de Bordeaux", "Dalmaty", "A Chipel, Lisa, Short Bridge, Desert Queen, St. Peter, Sultan, Pastier, Grease de Tarascon, Biore Dauphin) The components contained in were extracted with water and analyzed by high performance liquid chromatography (HPLC).

(experimental method)
(1) Leaves near the center of the new tree were collected from the fig trees of the above varieties and stored frozen.
(2) Nine times the amount (by weight) of water was added to the frozen leaves and crushed with a mixer.
(3) The suspension was filtered through gauze, and then the supernatant obtained by centrifugation (500 g × 5 minutes) was filtered through a membrane filter having a pore size of 0.45 μm to obtain a sample for analysis (40 samples). .
(4) Each analytical sample was analyzed with an HPLC apparatus. The analysis conditions are shown below.
(A) Column: Phenomenex AQUA C18, particle diameter 5 μm, inner diameter 4.6 mm × length 250 mm
(B) Mobile phase A: 2% acetic acid (c) Mobile phase B: 0.5% acetic acid and acetonitrile mixed at a ratio of 1: 1 (d) Mobile phase flow rate: 1 mL per minute
(E) Mobile phase gradient: The ratio of mobile phase B was changed as follows according to the analysis time. Analysis start time: 10%, 20 minutes later: 24%, 40 minutes later: 30%, 60 minutes later: 55%, 75 minutes later: 100%, until 83 minutes later: 100%, 85 minutes later: 10%, Until 90 minutes (end of analysis): 10%.
(F) Column temperature: 30 ° C
(G) Detection of component: Absorbance at a wavelength of 250 nm was measured with an absorbance detector.
(H) Elution time of each component: Detected as peaks at IPG: around 29 to 30 minutes, mIPG: around 31 minutes, psoralen: around 69-70 minutes, and bergapten: around 75 minutes, respectively.

(Experimental result)
In water, IPG and mIPG, which are furocoumarin-related glycosides, were hardly extracted.

  Of the 40 varieties, 38 varieties excluding 2 varieties “Grease de Tarascon” and “Biore Dauphin” contained both psoralen and bergapten. As for HPLC chromatograms, the chromatograms of Sakurai Dofin (Fig. 3) and Koji (Fig. 4), which are economically cultivated in Japan for the production of fruits and tea leaves, etc., and furocoumarins (psoralen and Only the chromatograms of Grease de Tarascon (FIG. 5) and Biore Dauphin (FIG. 6) that did not contain bergapten were shown, and the remaining chromatograms were omitted.

[Extraction and analysis of fig leaf with 0.1% hydrochloric acid-added methanol]
Components contained in the leaves of each of the 40 varieties of figs were extracted with 0.1% hydrochloric acid-added methanol and analyzed by liquid chromatography mass spectrometry (LC-MS).

(experimental method)
(1) Leaves near the center of the new tree were collected from the fig trees of the above varieties and stored frozen.
(2) Frozen leaves were crushed and a 25-fold amount (weight ratio) of solvent (methanol added with 0.1% hydrochloric acid) was added.
(3) The ingredients were extracted at room temperature for 4 hours with occasional stirring.
(4) The suspension was filtered through filter paper, and then filtered through a membrane filter having a pore size of 0.45 μm to obtain a sample for analysis (40 samples).
(5) The analytical sample was analyzed with an LC-MS apparatus. The analysis conditions are shown below.
(A) Column: Phenomenex AQUA C18, particle diameter 5 μm, inner diameter 4.6 mm × length 250 mm
(B) Mobile phase A: 2% acetic acid (c) Mobile phase B: 0.5% acetic acid and acetonitrile mixed at a ratio of 1: 1 (d) Mobile phase flow rate: 1 mL per minute
(E) Mobile phase gradient: The ratio of mobile phase B was changed as follows according to the analysis time. Analysis start time: 10%, 20 minutes later: 24%, 40 minutes later: 30%, 60 minutes later: 55%, 75 minutes later: 100%, until 83 minutes later: 100%, 85 minutes later: 10%, Until 90 minutes (end of analysis): 10%.
(F) Column temperature: 30 ° C
(G) Liquid chromatograph: Absorbance at a wavelength of 250 nm was measured with an absorbance detector.
(H) Mass spectrum: m / z values of component ions were measured with a mass spectrometer under the following measurement conditions. Condition 1: Ionization method: ESI (electrospray method), measurement mass range: 50 to 1000 m / z, fragmentor voltage: 150 V, capillary voltage: 3500 V, nebulizer gas: N ₂ (60 psi), dry gas: N ₂ (12 L / Min, 350 ° C.), measurement ion: negative ion. Condition 2: ionization method: APCI (atmospheric pressure chemical ionization method), measurement mass range: 50 to 1000 m / z, fragmentor voltage: 100 V, capillary voltage: 3000 V, corona current: 4 A, nebulizer gas: N ₂ (60 psi), Dry gas: N ₂ (4 L / min, 350 ° C.), vaporization temperature: 350 ° C., measurement ions: positive ions.
(I) Identification of peak components: Psoralen and bergapten were identified by the retention time agreeing with the data of the standard substance.
(J) Elution time of each component: Detected as peaks around IPG: 29 to 30 minutes, mIPG: around 31 minutes, psoralen: around 69 to 70 minutes, and bergapten: around 75 minutes, respectively.

(Experimental result)
Of the 40 varieties, 38 varieties except for 2 varieties of “Grease de Tarascon” and “Biore Dauphin” contained both IPG and mIPG. As for liquid chromatograms, the chromatograms of Sakurai Dofin (Fig. 7) and Koji (Fig. 8), which are economically cultivated in Japan for the production of tea leaves, etc., and furocoumarin-related glycosides (IPG and miPG) Only the chromatograms of Grease de Tarascon (FIG. 9) and Biore Dauphin (FIG. 10) that did not contain) were shown, and the chromatograms of the remaining varieties were omitted.

  As shown in FIGS. 9 and 10, in the liquid chromatograms of “Grease de Tarascon” and “Biore Dauphin”, around 29 minutes (29.808 minutes) and around 30 minutes (30.173 minutes) A very small peak that was considered to be IPG was detected, but mIPG was not detected.

  When m / z of ions contained in the above-mentioned minimum peaks of “Grease de Tarascon” and “Biore Dauphin” was examined by a mass spectrometer, there was IPG as shown in FIGS. An ion of 365 m / z indicating that (see FIG. 11) was not clearly detected. Therefore, these minimum peaks are considered to be due to impurities other than IPG.

  From the above, it was found that the two varieties “Grease de Tarascon” and “Biore Dauphin” do not contain psoralen, bergapten, IPG and mIPG.

  The composition for eating and drinking according to the present invention and the medicinal composition are tea beverages, green juices, cooking ingredients, fruit liquors, which have no risk of causing health problems such as the onset of skin diseases caused by light and increased side effects of drugs, It can be used as a crude drug.

Claims (4)

  A composition for eating and drinking using fig varieties free from furocoumarins and furocoumarin-related glycosides.   The composition for eating and drinking according to claim 1, wherein the fig cultivar is Grise de Tarascon or Biolet Dauphine.   A medicinal composition made from fig varieties not containing furocoumarins and furocoumarin-related glycosides.   4. The medicinal composition according to claim 3, wherein the fig cultivar is Grise de Tarascon or Biolet Dauphine.

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