CN109200082A - A method of extracting from emblic, there is antioxidant activity to have Substance simultaneously - Google Patents
A method of extracting from emblic, there is antioxidant activity to have Substance simultaneously Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention discloses a kind of to extract the method that there is antioxidant activity to have Substance simultaneously from emblic.This method comprises: ethanol water is added after crushing emblic in (1)；(2) pH value of solution adjusting is carried out by inorganic acid or organic acid；(3) after heating up, stirring condition next time carries out ultrasonication；(4) crude extract is separated and is concentrated, the mixed extract based on polyphenolics, organic acid, polysaccharide, flavonoids and glycoside substance is obtained by vacuum drying.Extracting method simple process of the invention, it is easy to operate, it is high-efficient, it ensure that crude extract can contain with antioxidant activity as far as possible and have Substance, be suitble to industrialized production.
Description
Technical field
The invention belongs to technical field of natural product extraction, and one kind is extracted from emblic, and there is antioxidant activity to have simultaneously
There is the method for Substance.
Background technique
Emblic (scientific name: Phyllanthus emblica), Phyllanthaceae Leafflower is distributed in China, Taiwan, perfume (or spice)
The ground such as port, Jiangxi, Fujian, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan and South East Asia Mainland are grown on 200 meters of height above sea level extremely
2300 area is more common in mountainous region sparse woods, shrubbery, wasteland or gully area without shade, also there is artificial growth.Fruit can be eaten raw, taste
Acid, micro-puckery be bitter and sweet perfume (or spice), eat it is first bitter after it is sweet, still emblic taste in mouth can promote the production of body fluid to quench thirst.
Fruit contains tannin, wherein there is glucogallin, gallic acid, benzoaric acid, corilagin, original scolded
Sub- acid, lupeol, Chebulagic acid, chebulinic acid, chebulic acid, 3,6- digalloylglucoses, dry fruit contain glactaric acid.Pericarp contains
Gallic acid, phyllemblic acid, emblic phenol.Seed about, contains linolenic acid, linoleic acid, oleic acid, stearic acid, palm containing fixing oil in oil
Acid, myristic acid etc..
The value of emblic not only embodies nutritionally, it is often more important that there are also medical values, it is that the Ministry of Public Health promulgates
It is both food and a kind of fruit of drug.The whole world has 17 countries or nationality to use emblic in the traditional medicine of oneself.
Such as recorded at " the civil common herbal medicine in Kunming ":
1, emblic controls diphtheria: one jin of Yunnan olive, each one liang of radix scrophulariae, Radix Glycyrrhizae.Cold boiling water is steeped to frostwork is played, and frost is taken to be spread out with cotton paper
After drying, add two money of horse hair rough gentian powder, borneol five divides, and fries five money of gingko benevolence powder, blows larynx use.
2, emblic controls globefish poisoning: Yunnan olive, which is eaten it raw, gulps down juice, and can control fish-bone stalk larynx.
3, control asthma: Yunnan olive 20-a first boils Pigs Hearts lung, goes offscum that the cooked even soup of olive is added to eat again.
Such as record in " Fujian drug will ": control high blood pressure: 5~8 pieces of fresh fruit of fructus phyllanthi are eaten raw, are taken daily 2 times.
Also record in former Guangzhou army " common Chinese herbal medicine handbook ": emblic cures cold fever, cough, sore-throat, dry
Polydipsia, vitamin C deficiency: fresh emblic fruit ten to 30.It is decocted in water for oral dose.
Bleeding, diarrhea and diarrhea are treated with emblic in India, also because it is rich in Vitamin C, are barked in India A Yu
It is considered as the food materials promoted longevity in top medicine, promotes health as dietotherapy, India government also once brought confrontation scurvy with it.
Emblic is rich in organic acids such as polyphenoils such as gallic acids and ellagic acid, the function with anti-inflammatory and repair tissue
Effect.
It can be seen that these important uses of emblic focus primarily upon antioxidant activity and bacteriostatic activity possessed by it
These two aspects.
As a plant resources big country, plant species are richly endowed by nature in China, the research medicinal for plant and utilization
It is with a long history.But from the starting opposite with the research of pharmaceutical activity and the relationship of extractive technique of modern chemistry angle research plant component
Later, this also results in the bottleneck problem for restricting emblic development.
Application number CN100375609C discloses a kind of dripping pills of emblic leafflower fruit and preparation method thereof technology.One kind having heat-clearing
It moisturizes, relieving sore throat and acesodyne effect for the scorching pharmaceutical composition for hurting the treatment for diseases such as dry throat pain caused by saliva, especially relates to
And a kind of drug composition oral preparation being prepared as a raw material with the 3 taste Chinese medicine such as emblic, borneol, menthol.Background skill
Art issues the throat smoothing tablet with emblic that the preparation method in drug standards WS3-B-0284-90 is prepared according to portion, is a kind of with clear
Heat is moisturized, relieving sore throat and acesodyne effect, for the scorching oral tablet for hurting the treatment for diseases such as dry throat pain caused by saliva, through clinic
Verifying, it is curative for effect, it is the common drug that clinical and family is used to treat above-mentioned illness.It is drug standards WS3-B- below
The formula and technique and brief description provided in 0284-90: prescription: emblic 1600g, borneol 0.5g;Menthol lg preparation method: with
Upper three taste, emblic add water to cook three times, each 1.5h, collecting decoction, and filtration is condensed into the thick paste of the moisture containing 18-25%, and thousand
It is dry, it crushes, adds cane sugar powder and right amount of auxiliary materials, particle is made, low temperature drying is let cool, and borneol, menthol is added, and is mixed, is made.
Such drug compounding patent in, the use of emblic be will by conventional method-" emblic adds water to cook three
It is secondary, each 1.5h, collecting decoction, filtration, be condensed into the thick paste of the moisture containing 18-25%, thousand is dry, crush " extract after be added.It is this kind of
Long-time high temperature process method can destroy Cucumber chemical structure, and then change its activity.
Invent the production method production technology of a kind of emblic health-care beverage series of CN1078118A and emblic preserved fruit.It should
Invention specific production process be: after the emblic fruit chosen is rinsed well with tap water with 0.01~0.02% it is dilute high
Potassium manganate solution disinfection, then rinsed with tap water, it is then impregnated 2-7 minutes with 0.1~0.5% sodium hydrogensulfite, removes fruit
Astringent taste.
In invention of such emblic for food, in order to protect color or removal astringent taste that can apply to bisulfite
Sodium destroys oxidation-resisting structure.
Emblic extract involved in invention CN101032322A and the preparation method and application thereof, by emblic dry powder
The former extracting solution being obtained by extraction by microwave-assisted solvent.Emblic extract carries out ether, ethyl acetate extraction respectively.It obtains
It is a kind of using emblic polyphenol as the natural of main active.
It is different by the polarity of emblic subconstiuent in such invention, there will be antioxidative polyphenol to separate and extract
Come, but unfortunately being abandoned with more preferable Substance such as organic acid.
Existing above-mentioned techniques or methods process the deep development of emblic and using there is certain deficiency, so needing one
The new extracting method of kind with low-cost high-efficiency can preferably utilize emblic child resource.
Summary of the invention
It processes for deep development of the prior art to emblic and certain insufficient using existing, is produced into reduce
This, shortens and extracts disengaging time, increase operation rate, and the present invention provides extract that there is antioxidant activity to have simultaneously from emblic
There is the method for Substance.To realize that goal of the invention, the present invention adopt the following technical scheme that, this method comprises:
(1) ethanol water is added after crushing emblic；
(2) pH value of solution adjusting is carried out by inorganic acid or organic acid；
(3) after heating up, stirring condition next time carries out ultrasonication；
(4) crude extract is separated and is concentrated, obtained by vacuum drying with polyphenolics, organic acid, polysaccharide, flavonoids
And the mixed extract based on glycoside substance.Extracting method simple process of the invention, it is easy to operate, it is high-efficient, it ensure that thick
Extract can contain with antioxidant activity as far as possible and have Substance, be suitble to industrialized production.
Extracting method of the present invention:
After step (1) the emblic raw material crushes, by the ethanol water (concentration of alcohol of feed liquid weight ratio 1:3-20 investment
For 5-75%, preferably 20-45%), temperature is controlled at 35-60 DEG C.
The excessively high influence organic acid extraction of concentration of alcohol, concentration is too low and influences the yield of flavonoids and glycoside substance.Through trying
It tests obtained by data, concentration of alcohol should control as 15-75%, and 20-45% effect is best.
The step (2) produces the inorganic acid or organic acid progress pH value of solution adjusting that regulation allows by food or drug.
Ethanol water pH is adjusted to 2-6；
Step (3) leaching process is aided with ultrasonication, ultrasound intensity 6-12W/cm2, frequency of sound wave range is 2-
3MHz, time are 20-70 minutes.
Ultrasonic wave is a kind of elastic mechanical vibration wave, can destroy the cell of plant, penetrate into solvent in plant cell, from
And accelerate polyphenolics, organic acid, polysaccharide, flavonoids and the dissolution of glycoside substance in emblic.In my experimental exploring
It was found that ultrasonic parameters (frequency, sound intensity degree etc.) are mentioned in many experiments of ultrasonic extraction using different frequency harmony intensity etc.
Different results can all be obtained by taking.According to the discovery in experiment, ultrasound intensity 6-12W/cm2, frequency of sound wave range is 2-
3MHz, recovery rate highest.
The step (4) is separated and is concentrated to crude extract, is obtained by vacuum drying with polyphenolics, organic
Mixed extract based on acid, polysaccharide, flavonoids and glycoside substance.
Since drying carries out under vacuum, oxygen is few, therefore some oxidizable substances are protected, and then protects
Antioxidant activity in extract.
The present invention has the advantage that compared with previous invention
It is extracted, be ensure that in emblic with antioxidant activity and with Substance for solvent by ethanol water
It extracts simultaneously.
PH value of solution is extracted by inorganic acid or organic acid to adjust, and ensure that the knot of Polyphenols and organic acid in emblic
The integrality of structure then ensure that in emblic with antioxidant activity and with the high activity of Substance.
Cavitation effect, mechanical effect are generated by mechanical vibration wave 3. being aided with ultrasonication, the cell of plant is destroyed, makes
Solvent penetrates into plant cell, to accelerate with antioxidant activity and with the dissolution of Substance.Compared to tradition
Processing greatlys save the processing time, and effectively reduces extraction temperature.
4. some oxidizable substances are protected, and then are protected anti-oxidant in extract by vacuum drying
Activity.
Specific embodiment
The present invention is further elaborated combined with specific embodiments below, but the present invention is not limited to following embodiments.
Embodiment 1
It weighs 100g and crushes emblic raw material, it is water-soluble to put into the ethyl alcohol that prepared pH is 4.8 in advance by feed liquid weight ratio 1:10
Liquid.Solution temperature is controlled at 50-60 DEG C, is persistently stirred 40 minutes, and being aided with ultrasound intensity is 6-12W/cm2, frequency of sound wave model
Enclose the ultrasonic wave for 2MHz.
After 40 minutes, by being separated by solid-liquid separation, liquid is concentrated under the conditions of being not higher than 60 DEG C.Concentrate is in vacuum item
3.5 grams of yellowish-brown powder are dried to obtain under part.
Crude extract ingredient is analyzed by liquid chromatogram.Contain in crude extract: glucose alkaloid
(glucogallin), gallic acid (gallic acid), benzoaric acid (ellagic acid), corilagin
(corilagin), terchebin (terchebin), chebulinic acid (chebulinic acid) and 3,6 one two nutgall acyl grapes
Glucoside (3,6-digalloylglucose) and glactaric acid, flavone compound Quercetin (quercetin), flavonols, Chinese radix scutellariae
Plain (wogonin).
Chromatographic test strip part:
1. chromatographic column: Hypersil ODS, 5 μm, 125mm × 4mm
2. mobile phase: methanol aqueous solution (methanol: water, volume ratio 80:20)
3. flow velocity: 0.8mL/min
4. Detection wavelength: 275nm
5. column temperature: 40 DEG C
Quantify the antibacterial activity of crude extract by MTT colorimetric method.MTT full name is 3- (4,5- dimethylthiazole -2) -2,5- hexichol
Base tetrazole bromide, trade name: thiazolyl blue.It is a kind of dyestuff of yellow color.It is a kind of method for detecting cell survival and growth.
Its testing principle is that the succinate dehydrogenase in living cells mitochondria can make exogenous MTT be reduced to the bluish violet knot of water-insoluble
Brilliant first a ceremonial jade-ladle, used in libation (Formazan) is simultaneously deposited in cell, and dead cell is without this function.Dimethyl sulfoxide (DMSO) can dissolve in cell
First a ceremonial jade-ladle, used in libation, with enzyme-linked immunosorbent assay instrument in 490 nm.Its absorbance value is measured at wavelength, can reflect living cells quantity indirectly.?
Within the scope of certain cell number, it is directly proportional to cell number that MTT crystallizes the amount to be formed.This method be widely used in some bioactivity because
Activity determination, large-scale screening anti-tumor medicine, cell toxicity test and tumor radiosensitivity measurement of son etc..It
Feature is high sensitivity, economy.
Experimental procedure:
1. preparing culture medium: being inoculated with (staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium to each strain respectively
Pseudomonas aeruginosa) culture it is spare to three generations.It is diluted using 10 times of dilution methods, each concentration respectively makees two parallel laboratory tests.Using plate bacterium
It falls counting method and calculates bacterium colony, making colony forming unit (colony forming unit, CFU) is 1 x 106 - 5 x 106 /
mL
2. by 96 well culture plates disinfection after, it is every arrange the 1st hole be added 16%DMSO dissolution crude extract 0.1mL, sequentially add 2 times it is dilute
The extract solution for degree of releasing is to the 10th hole, and each Kong Jun is to hang bacterium solution 106CFU/mL adds to 0.2mL, and the 10th hole is used as control,
Every group three parallel.37 DEG C are set, 5%CO2 is incubated for 16-48 hours, is observed under inverted microscope.10 μ L MTT solution are added in every hole
(5 mg/ml, i.e. 0.5%MTT) continue to cultivate 4 h.
It when the micro- aobvious blue particle object of culture bottom plate, is centrifuged (1000 turns of x10 min), carefully sops up supernatant, every hole is added
100 μ L dimethyl sulfoxides, set 10 min of low-speed oscillation on shaking table, dissolve crystal sufficiently.In enzyme-linked immunosorbent assay instrument
OD570 nm(630nm calibration) each hole of measurement light absorption value.
A(%)=(1- various concentration medical fluid hole OD value/control wells OD value) x100
Crude extract has preferable antibacterial effect to staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium Pseudomonas aeruginosa
Fruit.
Minimum Mlc completely is respectively as follows: staphylococcus aureus 4%
Escherichia coli 4%
Bacillus stearothermophilus 4%
Pseudomonas aeruginosa 4%
Quantify the antioxidant activity of crude extract by DPPH radicals scavenging method.
This method is that have single electron according to DPPH free radical, there is the last one absorption, alcoholic solution spy purple at 517nm
Property.In the presence of having free radical scavenger, due to its single electron match and so that its absorption is faded away, fading extent and its
The electron amount of receiving is at quantitative relationship, thus available spectrophotometer carries out quick quantitative analysis.
Experimental procedure
1. being kept in dark place with the DPPH solution of dehydrated alcohol configuration 0.1mmol/L.The Vc solution for configuring 0.5mg/mL (is made
For control).The concentration that emblic crude extract also presses 0.5mg/mL is prepared.
2. 2mL test sample solution and 2mL DPPH solution are added in same test tube, shake up, dark place is quiet at room temperature
Its absorbance A sample is measured after setting 30min, while measuring 2mL DPPH solution and 2mL dehydrated alcohol is mixed
Absorbance A control, and 2mL test sample solution and the mixed absorbance A blank of 2mL dehydrated alcohol.
Clearance rate shows that more greatly oxidation resistance is stronger.
After tested: the DPPH radicals scavenging power of crude extract is 62.09%
Comparative example 1
It weighs 100g and crushes emblic raw material, it is water-soluble to put into the ethyl alcohol that prepared pH is 4.8 in advance by feed liquid weight ratio 1:10
Liquid.Solution temperature is controlled at 50-60 DEG C, is persistently stirred 40 minutes.
After 40 minutes, by being separated by solid-liquid separation, liquid is concentrated under the conditions of being not higher than 60 DEG C.Concentrate is in vacuum item
It is only 2.4 grams that yellowish-brown powder is dried to obtain under part.
Crude extract ingredient is analyzed by liquid chromatogram.Contain in crude extract: glucose alkaloid
(glucogallin), gallic acid (gallic acid), benzoaric acid (ellagic acid), corilagin
(corilagin), terchebin (terchebin), chebulinic acid (chebulinic acid) and 3,6 one two nutgall acyl grapes
Glucoside (3,6-digalloylglucose) and glactaric acid, flavone compound Quercetin (quercetin), flavonols, Chinese radix scutellariae
Plain (wogonin).
Quantify the antibacterial activity of crude extract by MTT colorimetric method.
Crude extract has preferable suppression to staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium Pseudomonas aeruginosa
Bacterium effect.
Minimum Mlc completely is respectively as follows: staphylococcus aureus 4%
Escherichia coli 4%
Bacillus stearothermophilus 4%
Pseudomonas aeruginosa 4%
Quantify the antioxidant activity of crude extract by DPPH radicals scavenging method.
After tested: the DPPH radicals scavenging power of crude extract is 64.12%
Comparative example 2
It weighs 100g and crushes emblic raw material, it is unused by feed liquid weight ratio 1:10 investment ethanol water prepared in advance
Acid carries out pH adjusting.Solution temperature is controlled at 50-60 DEG C, is persistently stirred 40 minutes.
After 40 minutes, by being separated by solid-liquid separation, liquid is concentrated under the conditions of being not higher than 60 DEG C.Concentrate is in vacuum item
It is only 1.8 grams that yellowish-brown powder is dried to obtain under part.
Crude extract ingredient is analyzed by liquid chromatogram.Contain in crude extract: glucose alkaloid
(glucogallin), gallic acid (gallic acid), benzoaric acid (ellagic acid), corilagin
(corilagin), terchebin (terchebin), chebulinic acid (chebulinic acid) and 3,6 one two nutgall acyl grapes
Glucoside (3,6-digalloylglucose) and glactaric acid, flavone compound Quercetin (quercetin), flavonols, Chinese radix scutellariae
Plain (wogonin).
Quantify the antibacterial activity of crude extract by MTT colorimetric method.
Crude extract has preferable suppression to staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium Pseudomonas aeruginosa
Bacterium effect.
Minimum Mlc completely is respectively as follows: staphylococcus aureus 6%
Escherichia coli 5%
Bacillus stearothermophilus 6%
Pseudomonas aeruginosa 6%
Minimum Mlc completely increased, and illustrate that bacteriostatic activity is declined.
Quantify the antioxidant activity of crude extract by DPPH radicals scavenging method.
After tested: the DPPH radicals scavenging power of crude extract is 63.80%
Comparative example 3
Weigh 100g and crush emblic raw material, by feed liquid weight ratio 1:10 investment prepared aqueous solution in advance, unused acid into
Row pH is adjusted.Solution temperature is controlled at 50-60 DEG C, is persistently stirred 40 minutes.
After 40 minutes, by being separated by solid-liquid separation, liquid is concentrated under the conditions of being not higher than 60 DEG C.Concentrate is in vacuum item
It is only 0.9 gram that yellowish-brown powder is dried to obtain under part.
Crude extract ingredient is analyzed by liquid chromatogram.Contain in crude extract: glucose alkaloid
(glucogallin), gallic acid (gallic acid), benzoaric acid (ellagic acid), corilagin
(corilagin), terchebin (terchebin), chebulinic acid (chebulinic acid) and 3,6 one two nutgall acyl grapes
Glucoside (3,6-digalloylglucose) and glactaric acid, flavone compound Quercetin (quercetin), flavonols, Chinese radix scutellariae
Plain (wogonin).
Quantify the antibacterial activity of crude extract by MTT colorimetric method.
Crude extract has preferable suppression to staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium Pseudomonas aeruginosa
Bacterium effect.
Minimum Mlc completely is respectively as follows: staphylococcus aureus 5%
Escherichia coli 5%
Bacillus stearothermophilus 5%
Pseudomonas aeruginosa 6%
Minimum Mlc completely increased, and illustrate that bacteriostatic activity is declined.
Quantify the antioxidant activity of crude extract by DPPH radicals scavenging method.
After tested: the DPPH radicals scavenging power of crude extract is that 45.62%. antioxidant activity declines to a great extent.
Comparative example 14
It weighs 100g and crushes emblic raw material, it is water-soluble to put into the ethyl alcohol that prepared pH is 4.8 in advance by feed liquid weight ratio 1:10
Liquid.Solution temperature is controlled at 90 DEG C, is persistently stirred 40 minutes.
After 40 minutes, by being separated by solid-liquid separation, liquid is concentrated under the conditions of being not higher than 90 DEG C.Concentrate is in vacuum item
It is only 2.5 grams that yellowish-brown powder is dried to obtain under part.
Crude extract ingredient is analyzed by liquid chromatogram.Contain in crude extract: glucose alkaloid
(glucogallin), gallic acid (gallic acid), benzoaric acid (ellagic acid), corilagin
(corilagin), terchebin (terchebin), chebulinic acid (chebulinic acid) and 3,6 one two nutgall acyl grapes
Glucoside (3,6-digalloylglucose) and glactaric acid, flavone compound Quercetin (quercetin), flavonols, Chinese radix scutellariae
Plain (wogonin).
Quantify the antibacterial activity of crude extract by MTT colorimetric method.
Crude extract has preferable suppression to staphylococcus aureus, Escherichia coli, bacillus stearothermophilus, bacterium Pseudomonas aeruginosa
Bacterium effect.
Minimum Mlc completely is respectively as follows: staphylococcus aureus 4%
Escherichia coli 4%
Bacillus stearothermophilus 4%
Pseudomonas aeruginosa 4%
Quantify the antioxidant activity of crude extract by DPPH radicals scavenging method.
After tested: the DPPH radicals scavenging power of crude extract is that 52.42%. antioxidant activity is declined.
Claims (4)
1. a kind of extract the method that there is antioxidant activity to have Substance simultaneously from emblic, this method comprises:
(1) ethanol water is added after crushing emblic；
(2) pH value of solution adjusting is carried out by inorganic acid or organic acid；
(3) after heating up, stirring condition next time carries out ultrasonication；
(4) crude extract is separated and is concentrated, obtained by freeze-drying with phenolic constituent, organic acid, polysaccharide, flavonoids and glycoside
Mixed extract based on substance.
2. according to claim 1 extract the method that there is antioxidant activity to have Substance simultaneously, feature
It is: the step (1) ethanol water, ethanol content 15--70%.
3. method according to claim 1 or 2, it is characterised in that: the step (1) passes through inorganic acid or organic acid soln
It is 3-6 acid solution that liquid, which is adjusted to pH, and temperature control is being not higher than 70 DEG C.
4. method according to claim 1 to 3, it is characterised in that: the step (2) is aided with ultrasonication, surpasses
Sound intensity degree is 6-12W/cm2, frequency of sound wave range is 2-3MHz.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110613131A (en) * | 2019-06-20 | 2019-12-27 | 中国林业科学研究院资源昆虫研究所 | Application of emblic leafflower fruit extract in preparing health attenuated food |
| CN110724172A (en) * | 2019-11-23 | 2020-01-24 | 北京中医药大学 | Method for preparing chebulagic acid by ethanol reflux method |
| CN114591381A (en) * | 2022-03-23 | 2022-06-07 | 集美大学 | Method for extracting corilagin from fructus Phyllanthi and application thereof |
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| CN106721795A (en) * | 2016-12-06 | 2017-05-31 | 上海应用技术大学 | A kind of yellow peach enzyme beverage and preparation method thereof |
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| CN110613131A (en) * | 2019-06-20 | 2019-12-27 | 中国林业科学研究院资源昆虫研究所 | Application of emblic leafflower fruit extract in preparing health attenuated food |
| CN110724172A (en) * | 2019-11-23 | 2020-01-24 | 北京中医药大学 | Method for preparing chebulagic acid by ethanol reflux method |
| CN114591381A (en) * | 2022-03-23 | 2022-06-07 | 集美大学 | Method for extracting corilagin from fructus Phyllanthi and application thereof |
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