JP2011067151A - Hydroxyhydroquinone production inhibitor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a container-packed coffee drink in which the production of hydroxyhydroquinone is inhibited. <P>SOLUTION: The hydroxyhydroquinone production inhibitor for the container-packed coffee drink contains at least one kind selected from pyridoxal and pyridoxamine as an active ingredient. <P>COPYRIGHT: (C)2011,JPO&amp;INPIT

Description

  The present invention relates to a hydroxyhydroquinone production inhibitor for packaged coffee beverages.

  Coffee beverages contain chlorogenic acids such as chlorogenic acid, caffeic acid, and ferulic acid, which are polyphenols, and these chlorogenic acids are known to have excellent physiological activity. However, it has been reported that the physiological activity by chlorogenic acids is inhibited by hydroxyhydroquinone contained in coffee beverages.

  Therefore, it is advantageous to reduce the hydroxyhydroquinone content in the coffee beverage in order to effectively express the physiological activity due to chlorogenic acids. As such a coffee beverage, for example, a coffee beverage composition has been proposed in which the amount of chlorogenic acids in the coffee beverage is maintained within a certain range and the hydroxyhydroquinone content is reduced to a certain amount or less sufficiently smaller than the amount normally contained. (Patent Document 1).

  However, even if the amount of hydroxyhydroquinone in the coffee beverage is reduced as described above, there is a problem that hydroxyhydroquinone is easily generated in the heat sterilization process when the container is packed. Therefore, the amount of chlorogenic acids in the coffee beverage is kept within a certain range, and the hydroxyhydroquinone content is reduced to a certain amount or less sufficiently lower than the amount normally contained, and then the pH is kept within a certain range, or the coffee solids content is reduced. Container-packed coffee beverages (Patent Documents 2 and 3) in which the ratio of chlorogenic acids occupying a certain amount or more are proposed.

  On the other hand, in order to mask the bitterness of vitamins by paying attention to the functions of vitamins in the living body, a beverage in which vitamin B group is blended with a coffee extract and / or coffee flavor has been proposed (Patent Document 4). However, there is no precedent in which vitamins are added to coffee beverages in order to suppress the production of hydroxyhydroquinone after the coffee beverages are heat sterilized.

JP 2006-204192 A JP 2007-54061 A JP 2006-54062 A JP-A-5-146253

Therefore, the subject of this invention is providing the hydroxyhydroquinone production | generation inhibitor of a container-packed coffee drink.
Another object of the present invention is to provide a containerized coffee beverage in which the production of hydroxyhydroquinone is suppressed and a method for inhibiting the production of hydroxyhydroquinone in the containerized coffee beverage.

The present inventors have made various studies to suppress the formation of hydroxyhydroquinone packaged coffee beverage, by incorporating a specific vitamin B ₆ among Vitamin B ₆ compounds in coffee beverages, the packaged coffee beverage hydroxy It has been found that the production of kidroquinone is remarkably suppressed.

  That is, the present invention provides a hydroxyhydroquinone production inhibitor (hereinafter also referred to as “HHQ production inhibitor”) for a packaged coffee beverage, comprising at least one selected from pyridoxal and pyridoxamine as an active ingredient. .

The present invention also includes the following components (A) and (B):
(A) Chlorogenic acids: 0.01-1% by mass, and (B) a packaged coffee drink containing at least one selected from pyridoxal and pyridoxamine.

  The present invention further provides a packaged coffee beverage containing at least one selected from pyridoxal and pyridoxamine.

  The present invention still further provides a method for inhibiting the production of hydroxyhydroquinone in a packaged coffee beverage (hereinafter also referred to as “HHQ production inhibiting method”), wherein the hydroxyhydroquinone production inhibitor is added to chlorogenic acids.

ADVANTAGE OF THE INVENTION According to this invention, the agent and method which can suppress effectively the production | generation of the hydroxyhydroquinone of a packaged coffee drink are provided.
The container-packed coffee beverage of the present invention contains the above-mentioned hydroxyhydroquinone production inhibitor, so that the production of hydroxyhydroquinone is suppressed even during long-term storage where hydroxyhydroquinone is likely to be produced. It is hard to be inhibited by.

(Hydroxyhydroquinone production inhibitor for bottled coffee beverages)
The HHQ production inhibitor of the present invention comprises at least one selected from pyridoxal and pyridoxamine as an active ingredient.
Pyridoxal and pyridoxamine, which is an active ingredient of HHQ production inhibitor of the present invention is one vitamin B _6, vitamin B ₆ such is known as a physiologically active substance involved in the metabolism of amino acids and proteins in vivo.
Examples of vitamin B ₆ include pyridoxine, pyridoxal and pyridoxamine, and pyridoxine 5′-phosphate, pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate which are phosphate esters at the 5 ′ position thereof. In particular, the present inventors have found that pyridoxal and pyridoxamine specifically inhibit the production of hydroxyhydroquinone in a containerized coffee beverage.

  The pyridoxal and pyridoxamine used in the present invention may be those synthesized by known methods or commercially available products.

  The amount of the HHQ production inhibitor of the present invention added to the container-packed coffee beverage can be appropriately selected, but from the viewpoint of HHQ production inhibition and flavor, the upper limit is 0.2 mass%, and further 0.15 mass. %, More preferably 0.1% by weight, in particular 0.07% by weight, while the lower limit is 0.001% by weight, further 0.005% by weight, further 0.01% by weight, in particular 0.02% by weight. It is preferable that The production of hydroxyhydroquinone can be sufficiently suppressed with such a small addition amount.

  In addition, the same structure as the following can be employ | adopted for the container-packed coffee drink to which the HHQ production | generation inhibitor of this invention is added.

(Method for suppressing generation of hydroxyhydroquinone in containerized coffee beverages and containerized coffee beverages)
The packaged coffee beverage according to the present invention contains 0.01 to 1% by mass of (A) chlorogenic acids in the packaged coffee beverage, but from the viewpoint of physiological activity and flavor, the upper limit is 0.8% by mass, Further, it is preferably 0.6% by mass, more preferably 0.5% by mass, and particularly preferably 0.3% by mass, while the lower limit is 0.05% by mass, further 0.08% by mass, and further 0.1% by mass. In particular, the content is preferably 0.13% by mass. The content of chlorogenic acids is a value measured by “Analysis of chlorogenic acids” described in Examples below.
Here, in this specification, “(A) chlorogenic acids” means (A1) monocaffeoylquinic acid of 3-caffeoylquinic acid, 4-caffeoylquinic acid and 5-caffeoylquinic acid, and 3-caffeoylquinic acid, (A2) ferulquinic acid, 4-ferlaquinic acid and 3-ferlaquinic acid, and 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. (A3) A generic term that includes dicaffeoylquinic acid. The chlorogenic acid content is defined based on the total amount of the above nine types.

  Moreover, it is preferable that (C) hydroxyhydroquinone content is sufficiently reduced from the amount normally contained in the container-packed coffee beverage according to the present invention, and specifically, (C) hydroxy in the container-packed coffee beverage. The content of hydroquinone is less than 0.1% by mass and may be 0 with respect to (A) chlorogenic acids. From the viewpoint of inhibiting bioactivity inhibition and production efficiency, the upper limit of the content of (C) hydroxyhydroquinone in the container-packed coffee beverage is less than 0.05% by mass, more preferably 0.01% with respect to (A) chlorogenic acids. %, Further less than 0.005% by mass, further less than 0.001% by mass, further less than 0.0005% by mass, particularly preferably less than 0.0003% by mass. On the other hand, the lower limit is preferably 0.00001% by mass, particularly 0.0001% by mass, based on (A) chlorogenic acids. The content of hydroxyhydroquinone is a value measured by “analysis of hydroxyhydroquinone” described in Examples below, but is detected with high sensitivity because an electrochemical detector is used as a detection means. Moreover, in the analysis of the hydroxyhydroquinone by HPLC, you may measure after concentrating a container-packed coffee drink.

  Such a container-packed coffee beverage can be obtained by blending (C) a coffee extract with a sufficiently reduced hydroxyhydroquinone content and (A) adjusting the chlorogenic acid content.

A coffee extract with a sufficiently reduced hydroxyhydroquinone content can be obtained, for example, by the following method.
First, a coffee extract is prepared from coffee beans. In this case, instead of the coffee extract, an aqueous instant coffee solution, a liquid coffee extract, or the like may be used.
Although the kind of coffee bean used for extraction is not specifically limited, For example, Brazil, Colombia, Tanzania, Mocha, Kilimangelo, Mandelin, and Blue Mountain are illustrated. Examples of coffee bean types include Arabica and Robusta. One kind of coffee beans may be used, or a plurality of kinds may be blended and used.
It is preferable to use roasted coffee beans as the coffee beans, and examples of the roasting degree include light, cinnamon, medium, high, city, full city, French and Italian. Among these, light, cinnamon, medium, high, and city are preferable because they contain a large amount of chlorogenic acids and are easy to drink.
There are no particular restrictions on the method of roasting coffee beans, and there are no restrictions on the roasting temperature and roasting environment, and ordinary methods can be employed. Furthermore, there is no restriction | limiting also about an extraction method, For example, the method of extracting for 10 second-30 minutes using water or hot water (0-100 degreeC) from roasted coffee beans or its ground material is illustrated. Examples of the extraction method include a boiling type, an espresso type, a siphon type, and a drip type (paper, flannel, etc.).

  The coffee beans are preferably used in an amount of 1 g or more, more preferably 2.5 g or more, particularly 5 g or more in terms of green beans per 100 g of packaged coffee beverage.

Next, the resulting coffee extract is treated with an adsorbent to reduce the hydroxyhydroquinone content.
As the adsorbent, activated carbon, reverse phase chromatographic carrier, or the like can be used. More specifically, the adsorbent may be removed after adding the adsorbent to the coffee extract or the aqueous solution of the dried coffee extract and stirring at 0 to 100 ° C. for 10 minutes to 5 hours. The adsorbent is preferably used in an amount of 0.02 to 1.0 times in the case of activated carbon and 2 to 100 times in the case of a reverse phase chromatographic carrier with respect to the mass of coffee beans. As the activated carbon, those having an average pore radius in the micropore region of 5 angstroms (以下) or less, 2 to 5 angstroms, particularly 3 to 5 angstroms are preferable.

  Here, the micropore region means that the average pore radius is 10 angstroms or less, and the average pore radius is a value of the pore radius indicating the peak top of the pore distribution curve obtained by the MP method. And The “MP method” is a pore measurement method described in the literature (Colloid and Interface Science, 26, 46 (1968)).

Moreover, as a kind of activated carbon, coconut shell activated carbon is preferable, and also water vapor activated coconut shell activated carbon is preferable. As a commercial product of activated carbon, Shirakaba WH2C (Nippon EnviroChemicals Co., Ltd.), Taiko CW (Nimura Chemical Co., Ltd.), Kuraray Coal GW (Kuraray Chemical Co., Ltd.), etc. can be used. Examples of the reverse phase chromatographic carrier include YMC • ODS-A (YMC Corporation), C18 (GL Science Corporation) and the like.
Among these adsorbent treatment methods, the adsorbent treatment method using a specific activated carbon can selectively reduce the hydroxyhydroquinone content without lowering the chlorogenic acid content, and can be advantageously produced industrially. It is also preferable from the viewpoint of not reducing the potassium content (maintaining 1/5 or more, particularly 1/2 or more in mass ratio).

  The Brix value of the container-packed coffee beverage of the present invention is preferably 1 to 2, more preferably 1.1 to 1.8, and 1.2 to 1.6. A Brix value within the above range is preferred because of excellent flavor and difficulty in forming a precipitate. Here, the “Brix value” is a value measured by “Measurement of Brix” described in Examples described later.

For example, the following method can be used to adjust the Brix value.
1) A method for controlling the degree of roasting of coffee beans and the ratio of roasted beans and extract during extraction.
2) A method of extracting the fraction having a desired Brix value by dividing the extract obtained at the time of extraction into fractions.
3) A method of adsorbing specific components by activated carbon treatment.
4) A method for adjusting the brick value by separately adding an extract from low roasted beans or green beans.

  The total content of (B) pyridoxal and / or pyridoxamine in the packaged coffee beverage according to the present invention is 0.2% by mass, further 0.15% by mass, and further 0% from the viewpoints of HHQ production inhibition and flavor. 0.1% by mass, in particular 0.07% by mass, while the lower limit is 0.001% by mass, furthermore 0.005% by mass, further 0.01% by mass, in particular 0.02% by mass It is preferable.

In addition, the container-packed coffee beverage according to the present invention includes a milk component, a sweetener, a bitterness inhibitor, an antioxidant, a fragrance, an organic acid, an organic acid salt, an inorganic acid, an inorganic acid salt, an inorganic salt, and a dye as necessary. 1 type, or 2 or more types of additives, such as a kind, an emulsifier, a preservative, a seasoning, a sour agent, an amino acid, a pH adjuster, a quality stabilizer, may be mix | blended.
The container-packed coffee beverage of the present invention may be a black coffee beverage or a milk coffee beverage, but a black coffee beverage substantially free of milk components is preferred. The container-packed coffee beverage is preferably single-strength. Here, “single strength” means that after opening a packaged coffee beverage, it can be taken as it is without being diluted.

The container-packed coffee beverage according to the present invention has a content of H ₂ O ₂ (hydrogen peroxide) of 1 ppm or less, more preferably 0.1 ppm or less, even more preferably 0.05 ppm or less, from the viewpoint of suppressing the production of hydroxyhydroquinone and flavor. In particular, it is preferably 0.01 ppm or less. Here, the content of hydrogen peroxide is a value quantified by “measurement of hydrogen peroxide” described in Examples below. Although hydrogen peroxide is lost when a sealed container is filled with a coffee beverage and sterilized, it tends to gradually increase with time when the container is opened and exposed to air. Therefore, Japanese Patent No. 3732782 and Japanese Patent No. 3706339 Analyze quickly and promptly after opening in accordance with the measurement conditions described in the publication.

  The container-packed coffee beverage according to the present invention preferably has a pH (20 ° C.) of 5 to 7, more preferably 5.4 to 6.5, and particularly preferably 5.6 to 6.3.

Containers such as PET bottles, cans (aluminum, steel), paper, retort pouches, bottles (glass) and the like can be used for the container-packed coffee beverage according to the present invention. In this case, the container can be 50 to 2500 mL. The container is preferably a container having a low oxygen permeability from the viewpoint of preventing changes in the components in the coffee. For example, it is preferable to use a can such as aluminum or steel, a glass bottle, or the like. In the case of cans and bottles, recapable resealable types are also included. Here, “oxygen permeability” is the oxygen permeability (cc · mm / m ² · day · atm) measured in an environment of 20 ° C. and 50% relative humidity with a container / film oxygen permeability meter. The oxygen permeability is preferably 5 or less, more preferably 3 or less, and particularly preferably 1 or less.

The packaged coffee drink according to the present invention is preferably sterilized. The sterilization treatment can be manufactured under the sterilization conditions stipulated in the applicable regulations (Food Sanitation Law in Japan) if, for example, heat sterilization can be performed after filling a container such as a metal can. For those that cannot be sterilized by retort, such as PET bottles and paper containers, sterilize under the same sterilization conditions as above, for example, sterilize at high temperature and short time in a plate heat exchanger, and then cool to a certain temperature and fill the container. Etc. can be adopted.
According to the packaged coffee beverage of the present invention and the method for suppressing HHQ production of the packaged beverage, since the production of hydroxyhydroquinone is significantly suppressed even after heat sterilization, the physiological activity due to chlorogenic acids is hardly inhibited by hydroxyhydroquinone. Furthermore, the long-term storage stability is also improved.

(1) Analysis of chlorogenic acids HPLC was used as an analytical instrument. The model numbers of the constituent units of the apparatus are as follows.
UV-VIS detector: L-2420 (Hitachi High-Technologies Corporation),
Column oven: L-2300 (Hitachi High-Technologies Corporation),
Pump: L-2130 (Hitachi High-Technologies Corporation)
Autosampler: L-2200 (Hitachi High-Technologies Corporation),
Column: Cadenza CD-C18 inner diameter 4.6 mm × length 150 mm, particle diameter 3 μm (Intact Co.).

The analysis conditions are as follows.
Sample injection volume: 10 μL,
Flow rate: 1.0 mL / min,
UV-VIS detector setting wavelength: 325 nm,
Column oven set temperature: 35 ° C
Eluent A: 0.05 M acetic acid, 0.1 mM 1-hydroxyethane-1,1-diphosphonic acid, 10 mM sodium acetate, 5 (V / V)% acetonitrile solution,
Eluent B: acetonitrile.

Concentration gradient condition Time Eluent A Eluent B
0.0 minutes 100% 0%
10.0 minutes 100% 0%
15.0 minutes 95% 5%
20.0 minutes 95% 5%
22.0 minutes 92% 8%
50.0 minutes 92% 8%
52.0 minutes 10% 90%
60.0 minutes 10% 90%
60.1 minutes 100% 0%
70.0 minutes 100% 0%

In HPLC, 1 g of a sample was precisely weighed, made up to 10 mL with eluent A, filtered through a membrane filter (GL chromatodisc 25A, pore size 0.45 μm, GL Sciences Inc.), and subjected to analysis.
Retention time of chlorogenic acids (unit: minutes)
(A1) Monocafe oil quinic acid: 5.3, 8.8, 11.6 total 3 points (A2) Ferlaquinic acid: 13.0, 19.9, 21.0 total 3 points (A3) Dicafe Oil quinic acid: 36.6, 37.4, 44.2 in total.
From the area values of the nine types of chlorogenic acids determined here, the content (mass%) of chlorogenic acids was determined using 5-caffeoylquinic acid as a standard substance.

(2) Analysis of hydroxyhydroquinone by HPLC-electrochemical detector The analytical instrument used was a Couloarray system (model 5600A, manufactured by ESA, USA) which is an HPLC-electrochemical detector (coulometric type). The names and model numbers of the constituent units of the apparatus are as follows.
Analytical cell: Model 5010, Couloarray Organizer,
Couloarray electronics module software: Model 5600A,
Solvent delivery module: Model 582, gradient mixer,
Autosampler: Model 542, pulse damper,
Degasser: Degasys Ultimate DU3003,
Column oven: 505
Column: CAPCELL PAK C18 AQ inner diameter 4.6 mm × length 250 mm Particle diameter 5 μm (Shiseido Co., Ltd.).

The analysis conditions are as follows.
Sample injection volume: 10 μL,
Flow rate: 1.0 mL / min,
Applied voltage of electrochemical detector: 0 mV,
Column oven set temperature: 40 ° C
Eluent C: 0.1 (W / V)% phosphoric acid, 0.1 mM 1-hydroxyethane-1,1-diphosphonic acid, 5 (V / V)% methanol solution,
Eluent D: 0.1 (W / V)% phosphoric acid, 0.1 mM 1-hydroxyethane-1,1-diphosphonic acid, 50 (V / V)% methanol solution.

  For preparing the eluents C and D, distilled water for high performance liquid chromatography (Kanto Chemical Co., Ltd.), methanol for high performance liquid chromatography (Kanto Chemical Co., Ltd.), phosphoric acid (special grade, Wako Pure Chemical Industries, Ltd.) )), 1-hydroxyethane-1,1-diphosphonic acid (60% aqueous solution, Tokyo Chemical Industry Co., Ltd.).

Concentration gradient condition Time Eluent C Eluent D
0.0 minutes 100% 0%
10.0 minutes 100% 0%
10.1 min 0% 100%
20.0 minutes 0% 100%
20.1 minutes 100% 0%
50.0 minutes 100% 0%

  After accurately weighing 5 g of the sample, it was made up to 10 mL with 0.5 (W / V)% phosphoric acid, 0.5 mM 1-hydroxyethane-1,1-diphosphonic acid, 5 (V / V)% methanol solution. The solution was centrifuged and the supernatant was used as an analysis sample. This supernatant was passed through Bond Elut SCX (solid phase filling amount: 500 mg, reservoir volume: 3 mL, GL Sciences Inc.), and about 0.5 mL of the first passage solution was removed to obtain a passage solution. The passing liquid was filtered through a membrane filter (GL chromatodisc 25A, pore size 0.45 μm, GL Sciences Inc.) and immediately subjected to analysis.

  In the analysis under the above conditions of the HPLC-electrochemical detector, the retention time of hydroxyhydroquinone was 6.38 minutes. From the obtained peak area value, hydroxyhydroquinone (Wako Pure Chemical Industries, Ltd.) was used as a standard substance, and the content (mass%) of hydroxyhydroquinone was determined.

(3) Measurement of Brix The sample was measured for the refractometer reading (Brix) for sugar at 20 ° C. using a sugar meter (Atago RX-5000 (manufactured by Atago)).

(4) Measurement of hydrogen peroxide After calibrating with a standard calibration solution (hydrogen peroxide 1ppm) using a hydrogen peroxide analyzer (SUPER ORITECTOR MODEL 5, Central Science Co., Ltd.), in the analyzer measurement cell, 1 mL of 0.2 M phosphate buffer (pH 7.0) containing 0.5% potassium bromate was added. Nitrogen was supplied into the cell, and when the dissolved oxygen in the cell became zero, 1 mL of the sample was quickly extracted from the cell that had been allowed to stand in a constant temperature bath at 30 ° C. and added to the measurement cell. Thereafter, the generated oxygen concentration was read from the printer according to the measurement procedure of the apparatus. Thereafter, measurement was performed every 15 minutes, and a straight line was drawn by the least square method using the data obtained up to 1 hour later to determine the generation rate. Here, the detection limit of MODEL5 was 0.1 mg / kg.

(5) Sensory test About the presence or absence of the change of the flavor of each container-packed coffee drink before and behind storage at 60 degreeC for 2 weeks, it evaluated by five panelists, and determined by consultation after that.

Example 1
Using a multistage extractor, medium roasted coffee beans were extracted with 95 ° C. ion-exchanged water to obtain a coffee extract. Next, Brix in the coffee extract was measured, and a column (inner diameter: 45 mm, length: 150 mm) filled with activated carbon (Shirakaba WH2C, Nippon Envirochemicals Co., Ltd.) in an amount of 50% by mass with respect to Brix was prepared. . Thereafter, the coffee extract is passed through a column filled with activated carbon under the conditions of a temperature of 25 ° C. and SV3 [1 / volume [m ³ ] / flow rate [m ³ / hr]] and treated with activated carbon to remove hydroxyhydroquinone. A coffee extract was obtained.
Next, the obtained coffee extract from which hydroxyhydroquinone was removed was diluted with ion-exchanged water, pH was adjusted with sodium bicarbonate so that the pH value after heat sterilization treatment was 6.2, and further pyridoxal was added. . In addition, the hydroxyhydroquinone before heat sterilization was below the detection limit.
Next, after filling the obtained coffee composition into a 190 g can, it was sealed and subjected to a retort sterilization treatment (135 ° C., 100 seconds) to obtain a container-packed coffee beverage. And the analysis of the amount of chlorogenic acid and hydroxyhydroquinone after heat sterilization, the brick value, and the measurement of hydrogen peroxide were performed. Subsequently, after storing the obtained container-packed coffee beverage at 60 ° C. for 2 weeks, analysis of the chlorogenic acid amount and hydroxyhydroquinone amount after storage and a sensory test were performed. The results are also shown in Table 1.

Example 2
A packaged coffee beverage was produced in the same manner as in Example 1 except that the addition amount of pyridoxal was changed. And the container-packed coffee drink before and after preservation | save at 60 degreeC for 2 weeks was analyzed, and the sensory test was done. The results are also shown in Table 1.

Example 3
A packaged coffee drink was produced in the same manner as in Example 1 except that pyridoxal was replaced with pyridoxamine. And the container-packed coffee drink before and after preservation | save at 60 degreeC for 2 weeks was analyzed, and the sensory test was done. The results are also shown in Table 1.

Example 4
A packaged coffee beverage was produced in the same manner as in Example 1 except that pyridoxal was changed to pyridoxamine and the addition amount thereof was changed. And the container-packed coffee drink before and after preservation | save at 60 degreeC for 2 weeks was analyzed, and the sensory test was done. The results are also shown in Table 1.

Comparative Example 1
A packaged coffee beverage was produced in the same manner as in Example 1 except that pyridoxal was replaced with pyridoxine. And the container-packed coffee drink before and after preservation | save at 60 degreeC for 2 weeks was analyzed, and the sensory test was done. The results are also shown in Table 1.

Comparative Example 2
A packaged coffee beverage was produced in the same manner as in Example 1 except that pyridoxal was not blended. And the container-packed coffee drink before and after preservation | save at 60 degreeC for 2 weeks was analyzed, and the sensory test was done. The results are also shown in Table 1.

  As shown in Table 1, in the packaged coffee beverage in which the concentrations of chlorogenic acids and hydroxyhydroquinone are controlled to be constant and pyridoxal or pyridoxamine is blended, not only the production of hydroxyhydroquinone immediately after heat sterilization is suppressed, It was found that the production of hydroxyhydroquinone after storage at 60 ° C. for 2 weeks was significantly suppressed.

Claims (9)

  A hydroxyhydroquinone production inhibitor for packaged coffee beverages, comprising at least one selected from pyridoxal and pyridoxamine as an active ingredient.   The hydroxyhydroquinone production inhibitor of Claim 1 whose addition amount to the container-packed coffee drink of the said hydroxyhydroquinone production inhibitor is 0.001-0.2 mass%.   The hydroxyhydroquinone production inhibitor according to claim 1 or 2, wherein the packaged beverage contains 0.01 to 1% by mass of chlorogenic acids. The following components (A) and (B):
(A) Chlorogenic acids: 0.01 to 1% by mass, and (B) a packaged coffee drink containing at least one selected from pyridoxal and pyridoxamine.   The container-packed coffee drink of Claim 4 whose content of the said component (B) in the said container-packed coffee drink is 0.001-0.2 mass%.   The container-packed coffee drink according to claim 4 or 5, wherein the container-packed coffee drink has been subjected to a heat sterilization treatment.   The container-packed coffee drink of any one of Claims 4-6 whose Brix value of the said container-packed coffee drink is 1-2.   A packaged coffee drink containing at least one selected from pyridoxal and pyridoxamine.   The production | generation suppression method of the hydroxyhydroquinone of a container-packed coffee drink which adds the hydroxyhydroquinone production | generation inhibitor of any one of Claims 1-3 to chlorogenic acids.