JP2011063556A - Coaggregation inhibitor for oral cavity bacteria and composition for oral cavity compounded therewith - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an agent important for radically and effectively preventing periodontosis and dental caries and for inhibiting or delaying the progress or curing the diseases, and inhibiting all coaggregation of initial colonization bacteria and bacteria of genus Fusobacterium, bacteria of genus Fusobacterium and periodontosis-relating bacteria, and initial colonization bacteria and periodontosis-relating bacteria, and to provide an oral cavity composition compounded with the agent. <P>SOLUTION: The coaggregation inhibitor for oral cavity bacteria comprises dried powder and/or solvent extract of a plant selected from (A) one or more plants selected from Paeonia lactiflora, Humulus lupulus and Hamamelis virginiana, and/or (B) a combination of one or more plants selected from Crataegus cuneata, Crataegus laevigata and Aesculus hippocastanum with one or more plant selected from Salvia officinalis, Anthemis nobilis and Mentha piperita and/or (C) a combination of Houttuynia cordata with one or more plants selected from Crataegus cuneata, Crataegus laevigata and Aesculus hippocastanum. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a novel coaggregation inhibitor of oral bacteria. Specifically, coaggregation inhibitors that inhibit all coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, early colonization bacteria and periodontal disease related bacteria, and oral compositions containing the same Related to things.

  There are more than 500 types of bacteria in the oral cavity, and several types of bacteria coexist on the surface such as the tooth surface by forming aggregates (plaques). Such adhesion between other bacterial species is called coaggregation. Inhibiting the formation of this coaggregation in advance is important for the prevention and treatment of caries and periodontal disease. Plaques are first formed on the surface of the enamel covered with a thin film of saliva (pellicle), Streptococcus gordonii, Streptococcus oralis, Streptococcus sanguis, Actinomyces und It begins by adsorbing early colonized bacteria such as. Next, Fusobacterium genus bacteria represented by Fusobacterium nucleatum coaggregate, and the microbial flora transitions from facultative anaerobes to obligate anaerobes. Periodontal disease related bacteria such as Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia (Prevotella intermedia) co-aggregate and settle in Fusobacterium spp. It has been. It has also been clarified that early colonization bacteria and periodontal disease related bacteria coaggregate like Streptococcus gordonii and Porphyromonas gingivalis.

  Coaggregation is caused by non-specific electrostatic interactions between bacteria, lectin / receptor-type interactions, adhesion by sticky polysaccharide synthesis, and non-specific hydrophobic interactions. It plays an especially important role in settlement. Therefore, if the coaggregation of oral bacteria can be inhibited, various caries and periodontal pathogenic factors released from plaque can be eliminated in advance.

  It has been known so far that various plant solvent extracts have anti-cariogenic and anti-periodontal disease effects. For example, peony extract has been reported to have antibacterial activity and collagenase activity inhibitory action (see, for example, Patent Document 1). It is known that a hop extract has a methionase activity inhibitory action (for example, refer patent document 2). An inhibitory effect on insoluble glucan formation is known in the extracts of Hamelis extract and Hypericum perforatum (see, for example, Patent Document 3). An inhibitory action of protease activity is known in the dokudami extract and hawthorn extract (see, for example, Patent Document 4). The composition for oral cavity containing the malonie tannin and the licorice extract is disclosed as the Maronie extract (see, for example, Patent Document 5), and the hawthorn extract is known to inhibit methionase activity and protease activity. (For example, refer to Patent Document 6). As a sage extract, an oral composition comprising ε-polylysine / or a salt thereof and a sage extract is disclosed (for example, see Patent Document 7). Roman chamomile extract has disclosed a dentifrice composition that contains roman chamomile extract and is highly safe to living bodies and the environment (see, for example, Patent Document 8). An inhibitory action on trypsin activity is known in mint extract (see, for example, Patent Document 9).

  In addition, as a method for inhibiting colonization of pathogenic bacteria on the tooth surface, a specific amino acid is used to suppress coaggregation of oral bacteria (see, for example, Patent Documents 10 and 11), xanthan gum, gum tragacanth, Inhibiting bacterial aggregation using guar gum or the like (for example, see Patent Document 12), a plant selected from bodaiju, cinnamon, grape, chanoki, rose, cranberry, cacao, apple, ginkgo, loquat and evening primrose Inhibitors for inhibiting coaggregation of oral bacteria consisting of a dry powder or a solvent extract have been reported (see, for example, Patent Document 13).

However, it is important to fundamentally and effectively prevent periodontal disease and dental caries and to inhibit, delay, or treat the progression of early colonization and Fusobacterium, Fusobacterium and periodontal disease, It has not been realized to inhibit all coaggregation of colonized bacteria and periodontal disease related bacteria. In addition, antibacterial agents are widely used for the purpose of preventing periodontal disease and caries. However, the antibacterial agent shows a high bactericidal action when the bacterium is present alone, but it is not easy to penetrate and sterilize a substance that is an aggregate of a large number of bacteria such as plaque, and it is killed. Even if it is a bacterium, it may become a nucleus in bacterial aggregation, so it cannot be a fundamental solution.
JP-A-11-279039 JP 2003-160459 A JP 2004-196756 JP2003-335648 JP-A-11-222419 JP2002-3353 JP 2004-123630 A JP 2000-128753 A JP 2006-182732 A JP-A-2005-53851 JP-A-2005-53852 US Pat. No. 7,349,772 JP 2006-199661 A

  The present invention fundamentally and effectively prevents periodontal disease and dental caries, and is important for preventing, delaying or treating the progression of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria. An object of the present invention is to provide an inhibitor capable of inhibiting all coaggregation of early colonization bacteria and periodontal disease-related bacteria, and an oral composition containing the same.

  Therefore, the present inventors inhibit periodontal disease and caries by inhibiting all coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, and early colonization bacteria and periodontal disease related bacteria. As a result of intensive research to obtain an agent that can fundamentally and effectively prevent the disease, and inhibit or delay its progress, it has been found that a combination of a dry powder or a solvent extract of certain plants has such an effect. The present invention has been completed.

In detail,
A. At least one species of peonies, hops, and hamamelis inhibits all coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, and early colonization bacteria and periodontal disease related bacteria. It is possible to obtain the effect.
B. The combination of one or more of hawthorn, hawthorn, maronier and one or more of sage, roman chamomile, mint, Bacteria, Fusobacterium spp. And periodontal disease related bacteria are inhibited, and one or more of sage, Roman chamomile and mint can inhibit co-aggregation of early colonization bacteria and periodontal disease related bacteria. The effect of inhibiting coaggregation is obtained.
C. A combination of one or more of hawthorn, hawthorn, and maroni and dokudami, which inhibits co-aggregation of fusobacterium bacteria and periodontal disease related bacteria, early colonization bacteria and periodontal disease related bacteria, One or more species of hawthorn and maroni have the effect of inhibiting all coaggregation by inhibiting coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria.

That is, the present invention
A. One or more of peonies, hops, hamamelis and / or
B. A combination of and / or one or more of hawthorn, hawthorn, maronier and / or one or more of sage, roman chamomile, or mint
C. It is a coaggregation inhibitor of oral bacteria consisting of a dry powder and / or solvent extract of a plant selected from a combination of at least one of hawthorn, hawthorn hawthorn, and maroni. And it is the composition for oral cavity which mix | blended the coaggregation inhibitor of these oral bacteria.

  The inhibitor according to the present invention and a composition for oral cavity containing the same comprise an early colonization bacterium which is important for fundamentally and effectively preventing periodontal disease and caries and inhibiting, delaying or treating the progression of the disease. It is possible to inhibit all coaggregation of Fusobacterium, Fusobacterium and periodontal disease related bacteria, early colonization bacteria and periodontal disease related bacteria.

  As used herein, “periodontal disease-related bacteria” refers to bacteria known to be involved in periodontal disease, such as Porphyromonas gingivalis, Aggregate bacter actinomycetemcomi Tances (Aggregatibacter actinomycetemcomitans), Prevotella intermedia, Eikenella corrodens, Selenomonas sputigena, Campylobacter rectus, Camperlobacter rectum, There are spirochetes such as Treponema denticola. Examples of “Fusobacterium genus bacteria” used in the present specification include those present in the oral cavity such as Fusobacterium nucleatum and Fusobacterium periodonticum.

  As used herein, “early colonized bacteria” refers to bacteria attached to the pellicle covering the tooth surface, such as Streptococcus gordonii, Streptococcus oralis, Streptococcus sanguis, Actinomy Known is Actinomyces naeslundii.

  As used herein, “plant” refers to any tissue that constitutes a plant. For example, in addition to the whole collected plant, its flowers, ears, pericarp, fruits, stems, leaves, branches, branches and leaves, stems. , Bark, rhizome, root bark, root, seed. In addition, plants used in the agent of the present invention usually dry and pulverize plants such as flowers, flower spikes, pericarp, fruits, stems, leaves, branches, branches and leaves, stems, bark, rhizomes, root barks, roots, seeds, etc. It can be used in the form of a dry powder or an extract obtained by extracting them with a solvent. As the solvent extract, the above plant or dry powder is used, for example, water, methanol, ethanol, n-propanol, butanol, acetone, pyridine, polyethylene glycol, propylene glycol, chloroform, ethyl acetate, diethyl ether, benzene, toluene, cyclohexane. It was obtained by immersing in one or more solvents selected from carbon tetrachloride, etc., and allowing to stand for 1 to 7 days, or by stirring and extracting at 0 to 200 ° C., followed by filtration. It can be obtained by concentrating and drying the liquid. In this invention, you may mix | blend combining 1 type, or 2 or more types of the dry powder and solvent extraction extract of this plant body.

  The dry powder and solvent extract of the plant include peony peony liquid (Ichimaru Falcos Co., Ltd.), Falco Rex peonies B (Ichimaru Falcos Co., Ltd.), hop hop liquid (Ichimaru Falcos Co., Ltd.), Hammamel Falco Rex Hammamelis B (Ichimaru Falcos Co., Ltd.), Hawthorn Falcolex Hawthorn B (Ichimaru Falcos Co., Ltd.), Falcolex Hawthorn E (Ichimaru Falcos Co., Ltd.), Hawthorn Falcorex Hawthorn B (Ichimaru Falcos Co., Ltd.) Hawthorn E (Ichimaru Falcos Co., Ltd.), Eco-Farm Hawthorn E (Ichimaru Falcos Co., Ltd.), Cosmelene of Hawthorm (Nikko Chemicals Co., Ltd./GREENTECH SA), Falco Rex Maronie B (Ichimaru Fa Kos Co., Ltd., Escin / β-sitosterol Phytosome (Nikko Chemicals Co., Ltd./Indena SpA), Hypericum Falco Rex Hypericum B (Ichimaru Falcos Co., Ltd.), Falco Rex Hypericum E (Ichimaru Falcos Co., Ltd.), Sage Falco Rex Sage B (Ichimaru Falcos Co., Ltd.), Falco Rex Sage E (Ichimaru Falcos Co., Ltd.), Eco Farm Sage E (Ichimaru Falcos Co., Ltd.), Roman Chamomile Falco Rex Roman Chamomile B (Ichimaru Falcos Co., Ltd.) Peppermint B (Ichimaru Falcos Co., Ltd.), Falco Rex Peppermint PO (Ichimaru Falcos Co., Ltd.), Dokudami Falcolex Dokdami B (Ichimaru Falcos Co., Ltd.), Falcolex Dokdami E (Ichimaru Falcos Co., Ltd.) Lucolex Dokdami W (Ichimaru Falcos Co., Ltd.) is commercially available.

  The oral bacterial coaggregation inhibitor according to the present invention may be a dry powder or a solvent extract, which may be used as it is. If the solvent extract is a liquid, it may be concentrated or dried by a usual means such as reduced pressure drying or freeze drying. It may be used solidly. Moreover, you may refine | purify and use using a countercurrent distribution method, a liquid chromatography, etc. The dry powder or solvent extract of these plants is not particularly limited in the production area, production method, form, component composition, and the like.

  The oral bacteria coaggregation inhibitor of the present invention is preferably used as an oral composition. The composition for oral cavity is 0% as dry weight with respect to the total amount of the composition containing water or alcohol as a main component by freely combining one or more of the above dry powder and / or solvent extract. By adding 0.01 to 10% by weight, preferably 0.1 to 1% by weight, the effect of inhibiting coaggregation of oral bacteria is obtained. If the amount of the coaggregation inhibitor of oral bacteria is less than 0.01% by weight, it is difficult to obtain a satisfactory effect, while if it is more than 10% by weight, it may cause coloring / discoloration of the composition. This is not preferable because of its properties.

  The composition for oral cavity according to the present invention includes, for example, toothpaste, liquid dentifrice, mouthwash, oral rinse, low viscosity gel, dentifrice, oral cream, mouthwash, ointment, patch, oral gel, dragee It is possible to use in the form of etc.

  The composition for oral cavity according to the present invention is a solubilizer, surfactant, flavoring agent, sweetener, binding agent, abrasive, wetting agent, pH adjuster, and shaping, as long as the effects of the present invention are not impaired. Agents, lubricants, suspending agents, film-forming substances, flavoring or flavoring agents, coloring agents, preservatives, and other medicinal ingredients used in pharmaceutical compositions such as vitamins and conventional oral compositions It can mix | blend according to a conventional method.

  As the solubilizer, for example, ethanol, propylene glycol, butylene glycol, hexylene glycol and the like can be blended. Among these, ethanol and propylene glycol are particularly preferable from the viewpoint of use feeling. The blending amount of the solubilizer is preferably 0.1 to 30% by weight, particularly preferably about 1 to 10% by weight, based on the total amount of the oral composition. If it is less than 0.1% by weight, sufficient solubilizing power may not be obtained, and if it exceeds 30% by weight, it may be unfavorable in terms of use feeling.

  As the surfactant, an anionic surfactant, a nonionic surfactant, or an amphoteric surfactant can be blended. Examples of the anionic surfactant include alkyl sulfates such as sodium lauryl sulfate and sodium myristyl sulfate, acyl sarcosine salts such as sodium lauroyl sarcosinate and sodium myristoyl sarcosinate, acyl glutamate, palmitoyl glutamate, N-methyl- Examples thereof include acyl amino acid salts such as N-acyl taurine salt and N-methyl-N-acylalanine salt, coconut fatty acid monoglyceride monosulfate, lauryl sulfacetate, α-olefin sulfonate, sulfosuccinate and the like.

  Nonionic surfactants include, for example, sugar fatty acid esters such as sucrose fatty acid esters, maltose fatty acid esters, and lactose fatty acid esters, fatty acid alkanolamides, sorbitan fatty acid esters, fatty acid monoglycerides, fatty acid diethanolamides, polyoxyethylene addition moles of 8 And polyoxyethylene alkyl ether nonionic surfactants having 13 to 15 carbon atoms in the alkyl group, alkyl glycosides, and the like.

  Examples of the zwitterionic surfactant include N-alkyldiaminoethylglycine such as N-lauryldiaminoethylglycine, N-myristyldiaminoethylglycine, N-alkyl-N-carboxymethylammonium betaine, 2-alkyl-1-hydroxy Ethyl imidazoline betaine sodium, fatty acid amidopropyl betaine, N- [alkyl (1 2,1 4) oxy-2-hydroxypropyl] -L-arginine hydrochloride, lauryldimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl- N-hydroxyethyl imidazolinium betaine etc. are mentioned. These surfactants can be blended alone or in combination of two or more. The blending amount is 0.01 to 30% by weight, preferably 0.1 to 5% by weight, based on the total amount of the composition.

  Examples of the flavoring agent include menthol, carvone, anethole, eugenol, methyl salicylate, limonene, ocimene, n-decyl alcohol, citronell, α-terpineol, methyl acetate, citronenyl acetate, methyl eugenol, cineol, linalool, Ethyl linalool, thymol, spearmint oil, lemon oil, orange oil, rosemary oil, cinnamon oil, perilla oil, winter green oil, clove oil, eucalyptus oil, pimento oil, etc. can be blended. These flavoring agents can be blended alone or in combination of two or more. The blending amount is 0.1 to 10% by weight, preferably about 0.1 to 5% by weight, based on the total amount of the composition.

  Examples of the sweetening agent include sodium saccharin, acesulfame potassium, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perilartine, thaumatin, asparatylphenylalanylmethyl ester, p-methoxycinnamic aldehyde, and the like. These sweeteners can be used alone or in combination of two or more. The blending amount is 0.01 to 1% by weight, preferably 0.05 to 0.5% by weight, based on the total amount of the composition.

  Examples of the binder include cellulose derivatives such as sodium carboxymethylcellulose, sodium carboxymethylhydroxycellulose, hydroxyethylcellulose, hydroxypropylcellulose, cationized hydroxyethylcellulose, crystalline cellulose, alkali metal alginates such as sodium alginate, propylene glycol alginate, xanthan gum , Gums such as tragacanth gum, karaya gum, gum arabic, carrageenan, synthetic binders such as polyvinyl alcohol, sodium polyacrylate, carboxyvinyl polymer, polyvinyl pyrrolidone, inorganic binders such as silica gel, aluminum silica gel, bee gum, laponite, etc. Can be blended. These binders can be blended alone or in combination of two or more. The blending amount is 10% by weight or less based on the total amount of the composition.

  As the abrasive, for example, calcium hydrogen phosphate, calcium pyrophosphate, calcium carbonate, silica, alumina, aluminosilicate, aluminum hydroxide, zeolite, kaolin and the like can be blended. These abrasive | polishing agents can be mix | blended 1 type or in combination of 2 or more types. The blending amount is 0.1 to 30% by weight, preferably 1 to 10% by weight, based on the total amount of the composition.

  As the wetting agent, for example, sorbit, glycerin, diglycerin, ethylene glycol, 1,3-butylene glycol, polypropylene glycol, xylit, malt, lactit and the like can be blended. These wetting agents can be used alone or in combination of two or more. The amount is 5 to 70% by weight based on the total amount of the composition.

  As the pH adjuster, lactic acid, pantothenic acid, phosphoric acid, malic acid, citric acid, and salts thereof can be used.

  As vitamins, for example, vitamin C and salts thereof, vitamin E, vitamin A, β-carotene, vitamin D group, vitamin K group, vitamin P and the like can be blended.

  Excipients include sucrose, lactose, starch, glucose, crystalline cellulose, mannitol, sorbit, xylitol, erythritol, palatinit, palatinose, maltitol, trehalose, lactitol, reduced starch sugar, reduced isomaltoligosaccharide, coupling sugar , Gum base, gum arabic, gelatin, cetylmethylcellulose, light anhydrous silicic acid, magnesium aluminate, aluminate metasilicate, calcium, sodium bicarbonate, calcium phosphate, and the like.

  As the lubricant, magnesium stearate, talc, hardened oil, or the like can be blended. As suspending agents, coconut oil, olive oil, sesame oil, peanut oil, parsley oil, parsley seed oil, calcium lactate, safflower oil, soybean phospholipid, and the like can be blended.

  Examples of the film-forming substance include a carbohydrate derivative of cellulose acetate phthalate, an acrylic copolymer such as acrylic acid and methyl acrylate, and a methacrylic copolymer such as methacrylic acid and methyl methacrylate. it can.

  As a flavoring agent or flavoring agent, for example, saccharin sodium, aspartame, stevia extract, granulated sugar, powdered sugar, starch syrup, salt, orange oil, water-soluble licorice extract, menthol, eucalyptus oil and the like can be blended.

  Examples of other medicinal ingredients include vitamin E such as dl-α-tocopherol acetate, tocopherol succinate, tocopherol nicotinate, and cationic bactericides such as chlorhexidine, cetylpyridinium chloride, benzethonium chloride, and dodecyldiaminoethylglycine Amphoteric fungicides, nonionic fungicides such as triclosan and isopropylmethylphenol, enzymes such as dextranase, amylase, protease, mutanase, lysozyme, lytic enzyme, sodium monofluorophosphate, potassium monofluorophosphate Alkali metal monofluorophosphates such as sodium fluoride and stannous fluoride, and tranexamic acid, epsilon aminocaproic acid, aluminum chlorohydroxyl allantoin, dihydride Cholesterol, glycyrrhizin salts, glycyrrhetinic acid, glycerophosphate, chlorophyll, sodium chloride, can be blended Karopeputaido, a water-soluble inorganic phosphoric acid compounds and the like. These components can be blended alone or in combination of two or more.

<Sample>
Peonies Falco Rex Peonies B (Ichimaru Falcos Co., Ltd.)
Hop Hop Liquid (Ichimaru Falcos)
Hamelis Falco Rex Hamelis B (Ichimaru Falcos Co., Ltd.)
Dokudami Falco Rex Dokdami B (Ichimaru Falcos Co., Ltd.)
Hawthorn Falco Rex Hawthorn B (Ichimaru Falcos)
Hawthorn Falco Rex Hawthorn B (Ichimaru Falcos)
Maronie Falco Rex Maronie B (Ichimaru Falcos)
Sage Falco Rex Sage B (Ichimaru Falcos Co., Ltd.)
Roman chamomile Falco Rex Roman chamomile B (Ichimaru Falcos)
Peppermint Peppermint Peppermint B (Ichimaru Falcos Co., Ltd.)
Arnica Falco Rex Arnica (Ichimaru Falcos Co., Ltd.)
Ogon Ogon Liquid (Ichimaru Falcos Co., Ltd.)
Honeysuckle Falco Rex Honeysuckle SE (Ichimaru Falcos Co., Ltd.)
Turmeric Falco Rex Turmeric B (Ichimaru Falcos Co., Ltd.)
Cucumber Falco Rex Cucumber G (Ichimaru Falcos Co., Ltd.)
Carrot Falco Rex Carrot B (Ichimaru Falcos Co., Ltd.)
Dutch mustard Falco Rex Dutch mustard B (Ichimaru Falcos Co., Ltd.)
Burdock Falco Rex Burdock B (Ichimaru Falcos Co., Ltd.)
Odorikosou Falco Rex Odorikosou B (Ichimaru Falcos Co., Ltd.)
Tokin senka Falco Rex Tokin senka (Ichimaru Falcos Co., Ltd.)

Each of these test samples was diluted in ion exchange water to obtain a test solution.

<Test bacteria>
Streptococcus gordonii ATCC35105 (Streptococcus gordonii ATCC35105, Sg) was used as an early colonization bacterium.
Fusobacterium nucleatum ATCC25586 (Fusobacterium nucleatum ATCC25586, Fn) was used as a bacterium belonging to the genus Fusobacterium.
Porphyromonas gingivalis ATCC33277 (Pg) was used as a periodontal disease related bacterium.

Sg, Fn, and Pg were inoculated into Trypticase soy broth [vitamin K3 (0.5 mg / L), hemin (5 mg / L) added] medium, 37 ° C, 10% CO ₂ , 10% H ₂ , And cultured at 80% N ₂ from the logarithmic growth phase to the stationary phase. Each culture solution was centrifuged (12,000 rpm, 15 minutes, 4 ° C.) to obtain bacterial cells. Coaggregation buffers [Trishydroxymethylaminomethane (1 mmol / L), calcium chloride (0.1 mmol / L), magnesium chloride (0.1 mmol / L), sodium chloride (0.15 mol / L), Sodium azide (0.02%), ppH8] was added, and the cells were washed by centrifugation (12,000 rpm, 15 minutes, 4 ° C.). Thereafter, a coagulation buffer was added to the cells, and the cell suspension was prepared to 1.0 at OD660nm.

<Method for measuring coaggregation inhibitory activity>
Coaggregation is an aggregate obtained by combining two or more different bacteria. In the experiment, coaggregation was induced by three combinations of Pg and Fn, Fn and Sg, Pg and Sg. To 96 plates, 10 μL of the test solution and 50 μL of each of the two cell suspensions were added. Thereafter, using a microplate reader (Molecular Device Co., Ltd.), absorbance (OD660 nm) was measured at 40-second intervals for 30 minutes, and the absorbance change rate Vmax (mOD / min) was calculated. The coaggregation inhibition rate was calculated by the following formula using the value obtained when the test solution was used as Vmax-test, and the value obtained when ion-exchanged water was used instead of the test solution as Vmax-control.

  Coaggregation inhibition rate (%) = 100− (Vmax−test) / (Vmax−control) × 100

  Table 1 shows the coaggregation inhibition rate when tested at a concentration of 1% by weight. When the coaggregation suppression rate was 50% or more, it was determined that there was a coaggregation suppression effect (◯), and less than 50% was determined to have no coaggregation effect (×).

<Table 1>

As is clear from Table 1,
A. At least one species of peonies, hops, and hamamelis inhibits all coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, and early colonization bacteria and periodontal disease related bacteria. There is an effect to.
B. The combination of one or more of hawthorn, hawthorn, maronier and one or more of sage, roman chamomile, mint, Bacteria, Fusobacterium spp. And periodontal disease related bacteria are inhibited, and one or more of sage, Roman chamomile and mint can inhibit co-aggregation of early colonization bacteria and periodontal disease related bacteria. It has the effect of inhibiting coaggregation.
C. A combination of one or more of hawthorn, hawthorn, and maroni and dokudami, which inhibits co-aggregation of fusobacterium bacteria and periodontal disease related bacteria, early colonization bacteria and periodontal disease related bacteria, One or more species of hawthorn and maroni have the effect of inhibiting all coaggregation by inhibiting the coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria.

  On the other hand, the extract of Arnica planta from Arnica in Table 1 contains any combination of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, early colonization bacteria and periodontal disease related bacteria. The effect of inhibiting co-aggregation was not observed.

  Next, oral compositions capable of inhibiting all the coaggregation of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease related bacteria, and early colonization bacteria and periodontal disease related bacteria were prepared.

Example 1 <Mouthwash>
A mouthwash was manufactured according to the following formulation.

  About the obtained mouthwash, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Example 2 <Liquid dentifrice>
A liquid dentifrice was produced according to the following formulation.

  About the obtained liquid dentifrice, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Example 3 <Low viscosity gel>
A low viscosity gel was prepared according to the following formulation.

  About the obtained low-viscosity gel, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Example 4 <Dentifrice>
A dentifrice was manufactured according to the following prescription.

  About the obtained dentifrice, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Example 5 <Toothpaste>
A toothpaste was produced according to the following formulation.

  About the obtained toothpaste, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Example 6 <Oral Gel>
An oral gel was produced according to the following formulation.

  About the obtained gel for oral cavity, it evaluated by said co-aggregation evaluation test method. Excellent coaggregation inhibitory effects were demonstrated for all combinations of early colonization bacteria and Fusobacterium bacteria, Fusobacterium bacteria and periodontal disease-related bacteria, and early colonization bacteria and periodontal disease-related bacteria.

Claims (2)

A. One or more of peonies, hops, hamamelis and / or
B. A combination of and / or one or more of hawthorn, hawthorn, maronier and / or one or more of sage, roman chamomile, or mint
C. A coaggregation inhibitor of oral bacteria comprising a dry powder of a plant selected from a combination of at least one of hawthorn, hawthorn hawthorn, and maroni and a wolfberry and / or a solvent extract.   The composition for oral cavity which mix | blended the coaggregation inhibitor of the intraoral bacteria described in Claim 1.