JP2011011991A - Collagenase activity inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new collagenase activity inhibitor and a cosmetic containing the same.SOLUTION: The collagenase activity inhibitor comprises a tea flower extract that is conventionally not known to have an effect. The cosmetic comprises the collagenase activity inhibitor. The tea flower is a flower part of tea belonging to the genus Camellia of the family Theaceae and may contain pistil, stamen, sepal, floral axis, bracteal, bud, etc., besides petal. The cosmetic and the food each having effect of suppressing collagen decomposition and preventing skin aging are obtained by using the collagenase activity inhibitor.

Description

  The present invention relates to a collagenase activity inhibitor characterized by containing a tea flower extract.

  Collagen is the main component of the skin and is an elastic fibrous protein. If the amount of collagen production is normal, the skin becomes smooth with no blotches or dullness, elastic blood vessels are formed, and arteriosclerosis is less likely to occur. However, collagen is degraded in the body by the action of collagenase. Therefore, various substances that can be collagenase activity inhibitors that suppress collagen degradation and prevent skin aging have been studied.

To date, representative examples of collagenase activity inhibitors include caffeic acid or a salt thereof (Patent Document 1), guava leaf (Patent Document 2), Hamamelis extract (Patent Document 3), cowberry, birch, hamasuge, Bodaiju, green tea (patent document 4), spruce, mistletoe, jojoba leaf, lotus leaf, red clover (patent document 5), pine bark extract (patent document 6), kale (patent document 7), camcam seed extract (patent) Document 8), an extract of acerola seed (Patent Document 9) and the like are known.

JP-A-5-117145 JP-A-7-291873 JP-A-8-283133 JP 2003-12531 A JP 2003-48846 A JP 2003-238426 A JP 2003-238426 A Japanese Patent No. 3635081 JP 2007-314552 A

An object of this invention is to provide the novel raw material used for a collagenase activity inhibitor. Specifically, it is an object of the present invention to provide a collagenase activity inhibitor characterized by containing a tea flower extract, which has not been known to have the effect.

  As a result of studying various substances, the present inventors have found that a tea flower extract, which was not known to have a collagenase activity inhibitory effect, has the effect, and has completed the present invention.

That is, the present invention relates to a collagenase activity inhibitor characterized by containing a tea flower extract. Furthermore, the present invention relates to a cosmetic comprising the collagenase activity inhibitor.

  When a collagenase activity inhibitor characterized by containing a tea flower extract according to the present invention is used, it is possible to obtain cosmetics, foods, and the like having an effect of suppressing collagen degradation and preventing skin aging. It does not specifically limit regarding the form of this invention, A tea flower extract can be used as cosmetics, a foodstuff, etc. as it is or with various components added.

  Hereinafter, embodiments of the present invention will be described. The present invention should not be construed as being limited to the following embodiments, and various modifications can be made within the scope of the claims.

The tea flower used in the present invention is a tea belonging to the genus Camellia family Camellia sinensis, also known as Thea.
sinensis) and may include pistil, pistil, stamens, sepals, flower axes, camellia leaves, buds and the like. Tea is an evergreen tree that is widely grown or cultivated in China, Japan, Sri Lanka, etc., but the production area of tea used for tea flowers used in the present invention is not particularly limited.

  The tea flower extract used in the present invention can be extracted from the flower part of tea, but the extraction method is not particularly limited as long as the effect as a collagenase activity inhibitor is obtained, such as normal cosmetic raw materials Addition of extraction methods, extraction solvents, production aids and the like that can be used in the production of Moreover, the method of collect | recovering squeezed liquid by squeezing may be sufficient. Further, the obtained extract can be used after being dried by a conventional method. As a drying method, a method of adding an excipient and drying by spray drying or the like may be used. The tea flower extract may be an extract or a dry powder of the extract, and is not particularly limited.

  The tea flower extract of the present invention can be used as it is or as a cosmetic, food, etc. with various commonly used components added. If necessary, bases and additives usually used by those skilled in the art can be added.

  The tea flower extract used in the present invention as cosmetics can be processed into various forms such as lotions, emulsions, gels, creams, ointments and the like. It is also used as cosmetics, pharmaceuticals, quasi drugs and the like. Specifically, it can be used as a lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, body shampoo, facial cleanser, soap, foundation, spray, and the like.

The tea flower extract used in the present invention as a food is used by mixing with various components (for example, excipients, extenders, binders, thickeners, emulsifiers, colorants, fragrances, food additives, seasonings). it can. Specifically, royal jelly, vitamins, protein, chitosan, lecithin and the like are blended as nutritional supplements, and a sugar solution and seasoning can be added to adjust the taste. These may be formed into a capsule such as a hard capsule or a soft capsule, a tablet, or a pill, or a powder, granule, bowl, or the like as necessary. These may be eaten as they are, or may be drunk in water, hot water, milk, soy milk, tea, juice or the like according to the shape or preference.

  The blending amount of the tea flower extract contained in the collagenase activity inhibitor of the present invention is preferably 0.00001 to 50% by mass and more preferably 0.001 to 20% by mass as a dry weight. The blending amount can be appropriately set in a wide range according to various conditions such as.

The present invention will be described below with reference to examples, but it goes without saying that the present invention is not limited to these examples.

(Method for producing tea flower extract)
As the tea flower extract, 100 g of an extract obtained by adding 150 ml of a 50% water-containing 1,3-butylene glycol solvent to 10 g of a tea flower portion, heating at 60 ° C. for 1 hour or more, and filtering the extract was used.

(Example 1: Measurement of collagenase activity inhibition)
A tea flower extract was prepared using Phosphate buffered saline (PBS) (−) to prepare a sample solution. Collagenase B (Roche: Cat
No. 1088 807) was prepared using PBS (-) containing 0.1% BSA (bovine serum albumin) to a concentration of 10 µg / ml, and used as an enzyme solution. Fluorescent substrate dissolved in DMSO (Peptide Institute: Code
3163v) was prepared to 5 μM using PBS (−) containing 0.1% BSA, and used as a fluorescent substrate solution.

  The test solution was prepared as follows. Sample solution was added to a 96-well black plate at 50 μl / well. Thereafter, the enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Further, a fluorescent substrate solution was added at 50 μl / well, incubated at 37 ° C. for 60 minutes, then excited at 320 nm, and the fluorescence intensity at 405 nm was measured.

  The test solution blank was prepared as follows. Sample solution was added to a 96-well black plate at 50 μl / well. Thereafter, PBS (−) containing 0.1% BSA was added at 100 μl / well instead of the enzyme solution, and incubated at 37 ° C. for 10 minutes. Further, a fluorescent substrate solution was added at 50 μl / well, incubated at 37 ° C. for 60 minutes, then excited at 320 nm, and the fluorescence intensity at 405 nm was measured.

  The control solution was prepared as follows. PBS (−) was added to a 96-well black plate at 50 μl / well. Thereafter, the enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Further, a fluorescent substrate solution was added at 50 μl / well, incubated at 37 ° C. for 60 minutes, then excited at 320 nm, and the fluorescence intensity at 405 nm was measured.

  The control solution blank was prepared as follows. PBS (−) containing 0.1% BSA was added to a 96-well black plate at 50 μl / well. Thereafter, PBS (−) containing 0.1% BSA was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Further, a fluorescent substrate solution was added at 50 μl / well, incubated at 37 ° C. for 60 minutes, then excited at 320 nm, and the fluorescence intensity at 405 nm was measured.

The inhibition of collagenase activity was expressed by the inhibition rate obtained from the following formula.
Inhibition rate (%) = 100 − [(A−B) / (C−D)] × 100
A: Fluorescence intensity at 405 nm of the test solution.
B: Fluorescence intensity at 405 nm of the test solution blank.
C: Fluorescence intensity at 405 nm of the control solution.
D: Fluorescence intensity at 405 nm of the control solution blank.

  From the above results, it was clarified that the tea flower extract shows collagenase activity inhibition.

(Example of cosmetic production)
Using the tea flower extract, the following cosmetics were prepared by a conventional method.
Blending amount (% by mass)
Tea flower extract 0.02
Ethanol 10.0
Polyoxyethylene hydrogenated castor oil 1.0
Methyl paraoxybenzoate 0.1
Dipropylene glycol 5.0
1,3-butylene glycol 10.0
Purified water remaining

  The present inventors have found that the tea flower extract has collagenase activity inhibition and completed the present invention. The collagenase activity inhibitor described in the present invention can be used as cosmetics, foods, etc., with the tea flower extract as it is or with various components added.

Claims (2)

A collagenase activity inhibitor comprising a tea flower extract. A cosmetic comprising the collagenase activity inhibitor according to claim 1.