JP2010518885A - 並列記録能力を有するマルチネットワーク神経細胞分析プラットフォーム - Google Patents
並列記録能力を有するマルチネットワーク神経細胞分析プラットフォーム Download PDFInfo
- Publication number
- JP2010518885A JP2010518885A JP2009528265A JP2009528265A JP2010518885A JP 2010518885 A JP2010518885 A JP 2010518885A JP 2009528265 A JP2009528265 A JP 2009528265A JP 2009528265 A JP2009528265 A JP 2009528265A JP 2010518885 A JP2010518885 A JP 2010518885A
- Authority
- JP
- Japan
- Prior art keywords
- recording
- neural network
- well
- network analysis
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/023—Sending and receiving of information, e.g. using bluetooth
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2857—Seizure disorders; Epilepsy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Clinical Laboratory Science (AREA)
- Animal Behavior & Ethology (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
引用文献
以下の米国特許文献および公報を参考として本明細書に援用する。
他の文献
Gramowski, Schiffmann and Gross,
"Quantification of acute neurotoxic effects of trimethyltin using neuronal
networks cultured on microelectrode arrays," NeuroToxicology, 21:331-42,
2000.
Gross and Gopal, Emerging histiotypic
properties of cultured neuronal networks. In: M. Taketani and M. Baudry
(eds) Advances in Network Electrophysiology using Multi-Electrode Arrays.
Springer, pp 193-214. 2006.
Gross and Pancrazio Neuronal Network
Biosensors. In: Smart Biosensor Technology (G. K. Knopf and A. S. Bassi,
eds), Taylor and Francis Publishers, CRC Press.pp 177-201, 2006.
Gross, Rieske, Kreutzberg and Meyer,
"A new fixed-array multimicroelectrode system designed for longterm
monitoring of extracellular single unit neuronal activity in vitro,"
Neurosci. Lett., 6:101-05, 1977.
Gross, "Internal dynamics of
randomized mammalian neuronal networks in culture," In:Enabling
Technologies for Cultured Neural Networks, Stenger and McKenna (Eds.), Academic
Press, NY, pp. 277-317, 1994.
Gross, Harsch, Rhoades and Gopel,
"Odor, drug, and toxin analysis with neuronal networks in vitro:
extracellular array recording of network responses," Biosensors and
Bioelectronics, 12:373-93, 1997.
Keefer, Gramowski and Gross, "NMDA
receptor dependent periodic oscillations in cultured spinal cord
networks," J. Neurophysiol, 86:3030-3042, 2001a.
Keefer, Gramowski, Stenger, Pancrazio and
Gross, "Characterization of acute neurotoxic effects of trimethylolpropane
phosphate via neuronal network biosensors," Biosensors and Bioelectronics
16: 513-52, 2001b.
Claims (18)
- 複数の記録列で構成される複数の記録ウェルであって、各記録ウェルは、神経細胞回路網、および前記神経細胞回路網からの信号を記録するための複数の電極を含む、複数の記録ウェルと、
複数の増幅列で構成される複数の増幅ウェルであって、各増幅ウェルは、前記記録ウェルからの記録信号を増幅するための1つまたは複数の増幅器を含み、前記複数の記録列および前記複数の増幅列は、各記録ウェルが対応する増幅ウェルに隣接するように交互に並んでいる、複数の増幅ウェルと、
各相互接続線が、記録ウェルの前記電極を対応する増幅ウェルの前記増幅器と接続する、複数の相互接続線とを備える神経回路網解析プレート。 - 前記複数の記録ウェルは、6つの記録列で構成される48個の記録ウェルを備え、前記複数の増幅ウェルは、6つの増幅列で構成される48個の増幅ウェルを備える、請求項1に記載の神経回路網解析プレート。
- 前記神経回路網解析プレートは、8個のウェルそれぞれが12列を有する標準の96ウェル・プレートの各ウェルを利用する、請求項2に記載の神経回路網解析プレート。
- 前記複数の記録ウェルは、4つの記録列で構成される16個の記録ウェルを備え、前記複数の増幅ウェルは、2つの増幅列で構成される8個の増幅ウェルを備える、請求項1に記載の神経回路網解析プレート。
- 前記神経回路網解析プレートは、4個のウェルそれぞれが6つの列を有する標準の24ウェル・プレートの各ウェルを利用する、請求項4に記載の神経回路網解析プレート。
- 前記増幅ウェル内の前記増幅器と接触している複数の母線をさらに備える、請求項1に記載の神経回路網解析プレート。
- 前記母線はコネクタに接続され、前記コネクタは2次増幅装置に接続される、請求項6に記載の神経回路網解析プレート。
- ロボット液体ハンドリング・システムをさらに備える、請求項1に記載の神経回路網解析プレート。
- 1つまたは複数の作用物質にさらされている活動神経細胞回路網からの活動電位信号を、並列に非多重化して記録するための方法であって、
請求項1の神経回路網解析プレートを準備するステップと、
前記神経回路網解析プレートの記録ウェルを、前記1つまたは複数の作用物質にさらすステップと、
前記神経回路網解析プレートの増幅ウェルによって増幅された信号を記録するステップとを含む方法。 - 前記1つまたは複数の作用物質は、1つまたは複数の薬剤である、請求項9に記載の方法。
- 1つまたは複数の作用物質にさらされている活動神経細胞回路網からの活動電位信号を、並列に非多重化して記録するための方法であって、
請求項7の神経回路網解析プレートを準備するステップと、
前記神経回路網解析プレートの記録ウェルを、前記1つまたは複数の作用物質にさらすステップと、
2次増幅装置によって増幅された信号を記録するステップとを含む方法。 - 前記1つまたは複数の作用物質は、1つまたは複数の薬剤である、請求項11に記載の方法。
- 生体外で、1つまたは複数の作用物質に対する神経細胞の薬理反応および毒性反応を検出し定量化するための方法であって、
請求項1の神経回路網解析プレートを準備するステップと、
前記神経回路網解析プレートの記録ウェルを、前記1つまたは複数の作用物質にさらすステップと、
前記神経回路網解析プレートの増幅ウェルによって増幅された信号を記録するステップとを含む方法。 - 前記1つまたは複数の作用物質は、1つまたは複数の薬剤である、請求項13に記載の方法。
- 前記記録された信号を解析して、前記1つまたは複数の作用物質に対する前記神経細胞の前記薬理反応および毒性反応を定量化するステップをさらに含む、請求項13に記載の方法。
- 生体外で、1つまたは複数の作用物質に対する神経細胞の薬理反応および毒性反応を検出し定量化するための方法であって、
請求項5の神経回路網解析プレートを準備するステップと、
前記神経回路網解析プレートの記録ウェルを、前記1つまたは複数の作用物質にさらすステップと、
2次増幅装置によって増幅された信号を記録するステップとを含む方法。 - 前記1つまたは複数の作用物質は、1つまたは複数の薬剤である、請求項16に記載の方法。
- 前記記録された信号を解析して、前記1つまたは複数の作用物質に対する前記神経細胞の前記薬理反応および毒性反応を定量化するステップをさらに含む、請求項16に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US84539906P | 2006-09-18 | 2006-09-18 | |
US60/845,399 | 2006-09-18 | ||
PCT/US2007/019780 WO2008100287A2 (en) | 2006-09-18 | 2007-09-12 | Multinetwork nerve cell assay platform with parallel recording capability |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2010518885A true JP2010518885A (ja) | 2010-06-03 |
JP5232785B2 JP5232785B2 (ja) | 2013-07-10 |
Family
ID=39690646
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009528265A Expired - Fee Related JP5232785B2 (ja) | 2006-09-18 | 2007-09-12 | 並列記録能力を有するマルチネットワーク神経細胞分析プラットフォーム |
Country Status (7)
Country | Link |
---|---|
US (1) | US8460921B2 (ja) |
EP (1) | EP2064546B1 (ja) |
JP (1) | JP5232785B2 (ja) |
AT (1) | ATE487135T1 (ja) |
DE (1) | DE602007010321D1 (ja) |
ES (1) | ES2355528T3 (ja) |
WO (1) | WO2008100287A2 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9279801B2 (en) | 2013-07-26 | 2016-03-08 | Axion Biosystems, Inc. | Devices, systems and methods for high-throughput electrophysiology |
DE102014003432B3 (de) * | 2014-03-11 | 2015-05-07 | NeuroProof GmbH | Verfahren zum Testen neuroaktiver Substanzen |
WO2016025646A1 (en) | 2014-08-12 | 2016-02-18 | Axion Biosystems, Inc. | Cell-based biosensor array and associated methods for manufacturing the same |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08149998A (ja) * | 1994-11-28 | 1996-06-11 | Nippon Telegr & Teleph Corp <Ntt> | 細胞に対する薬剤等の効果を判定する装置及び方法 |
JP2002541859A (ja) * | 1999-04-15 | 2002-12-10 | デフヘン・ナムローゼ・フェンノートシャップ | 化合物スクリーニング方法 |
JP3570715B2 (ja) * | 2001-06-05 | 2004-09-29 | 松下電器産業株式会社 | マルチ電極を備えた信号検出用センサ |
WO2006130990A2 (en) * | 2005-06-10 | 2006-12-14 | Neurosilicon (1145990 Alberta Ltd.) | Detecting electrical activity and assessing agents for the ability to influence electrical activity in neuronal cell networks |
JP2007534927A (ja) * | 2003-07-14 | 2007-11-29 | ヴァーテックス ファーマシューティカルズ、 インコーポレイテッド | イオンチャネルアッセイ方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030154501A1 (en) * | 1999-04-15 | 2003-08-14 | Devgen, N.V. | Compound screening method |
US6969449B2 (en) * | 2000-07-10 | 2005-11-29 | Vertex Pharmaceuticals (San Diego) Llc | Multi-well plate and electrode assemblies for ion channel assays |
EP1802752B1 (en) * | 2004-09-10 | 2012-06-13 | Molecular Devices, LLC | Parallel patch clamp system |
EP1827207A2 (en) * | 2004-10-04 | 2007-09-05 | Cyberkinetics Neurotechnology Systems, Inc. | Biological interface system |
-
2007
- 2007-09-12 JP JP2009528265A patent/JP5232785B2/ja not_active Expired - Fee Related
- 2007-09-12 WO PCT/US2007/019780 patent/WO2008100287A2/en active Application Filing
- 2007-09-12 DE DE602007010321T patent/DE602007010321D1/de active Active
- 2007-09-12 ES ES07872615T patent/ES2355528T3/es active Active
- 2007-09-12 AT AT07872615T patent/ATE487135T1/de not_active IP Right Cessation
- 2007-09-12 US US12/441,703 patent/US8460921B2/en not_active Expired - Fee Related
- 2007-09-12 EP EP07872615A patent/EP2064546B1/en not_active Not-in-force
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08149998A (ja) * | 1994-11-28 | 1996-06-11 | Nippon Telegr & Teleph Corp <Ntt> | 細胞に対する薬剤等の効果を判定する装置及び方法 |
JP2002541859A (ja) * | 1999-04-15 | 2002-12-10 | デフヘン・ナムローゼ・フェンノートシャップ | 化合物スクリーニング方法 |
JP3570715B2 (ja) * | 2001-06-05 | 2004-09-29 | 松下電器産業株式会社 | マルチ電極を備えた信号検出用センサ |
JP2007534927A (ja) * | 2003-07-14 | 2007-11-29 | ヴァーテックス ファーマシューティカルズ、 インコーポレイテッド | イオンチャネルアッセイ方法 |
WO2006130990A2 (en) * | 2005-06-10 | 2006-12-14 | Neurosilicon (1145990 Alberta Ltd.) | Detecting electrical activity and assessing agents for the ability to influence electrical activity in neuronal cell networks |
Also Published As
Publication number | Publication date |
---|---|
US20090291466A1 (en) | 2009-11-26 |
US8460921B2 (en) | 2013-06-11 |
EP2064546B1 (en) | 2010-11-03 |
EP2064546A2 (en) | 2009-06-03 |
WO2008100287A2 (en) | 2008-08-21 |
DE602007010321D1 (de) | 2010-12-16 |
ES2355528T3 (es) | 2011-03-28 |
ATE487135T1 (de) | 2010-11-15 |
JP5232785B2 (ja) | 2013-07-10 |
WO2008100287A3 (en) | 2008-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Novellino et al. | Development of micro-electrode array based tests for neurotoxicity: assessment of interlaboratory reproducibility with neuroactive chemicals | |
Abbott et al. | CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging | |
Jones et al. | The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics | |
Jäckel et al. | Combination of high-density microelectrode array and patch clamp recordings to enable studies of multisynaptic integration | |
Johnstone et al. | Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century | |
Morin et al. | Investigating neuronal activity with planar microelectrode arrays: achievements and new perspectives | |
Margolis et al. | Reorganization of cortical population activity imaged throughout long-term sensory deprivation | |
Sato et al. | The functional microarchitecture of the mouse barrel cortex | |
Kerr et al. | Imaging input and output of neocortical networks in vivo | |
EP3494877B1 (en) | Device for the examination of neurons | |
McCready et al. | Multielectrode arrays for functional phenotyping of neurons from induced pluripotent stem cell models of neurodevelopmental disorders | |
US20180120294A1 (en) | Microfluidic Devices for Cells or Organ based Multimodal Activation and Monitoring system | |
Brofiga et al. | Functional inhibitory connections modulate the electrophysiological activity patterns of cortical-hippocampal ensembles | |
Briggs et al. | Laminar patterns of local excitatory input to layer 5 neurons in macaque primary visual cortex | |
JP5232785B2 (ja) | 並列記録能力を有するマルチネットワーク神経細胞分析プラットフォーム | |
Gholmieh et al. | A biosensor for detecting changes in cognitive processing based on nonlinear systems analysis | |
US10793820B2 (en) | Miniaturized, automated in-vitro tissue bioreactor | |
Bakker et al. | Classification of cortical microcircuits based on micro-electrode-array data from slices of rat barrel cortex | |
Obien et al. | CMOS-based high-density microelectrode arrays: technology and applications | |
DE60117180T2 (de) | Verfahren zur messung des aktivierungszustands von signalwegen in zellen | |
WO2001056647A1 (en) | Assays for the detection of agents that affect cognition | |
Frega | Neuronal Network Dynamics in 2D and 3D in Vitro Neuroengineered Systems | |
JP2014124133A (ja) | 細胞外電位計測装置及び方法 | |
Belbachir et al. | High-throughput analysis of drug safety responses in induced pluripotent stem cell-derived cardiomyocytes using multielectrode array | |
Wheeler | Designing in vitro patterned neuronal networks |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20100802 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130312 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130325 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160329 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |