JP2010273572A - Bacterium examination method and bacterium examination tool - Google Patents

Bacterium examination method and bacterium examination tool Download PDF

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JP2010273572A
JP2010273572A JP2009127100A JP2009127100A JP2010273572A JP 2010273572 A JP2010273572 A JP 2010273572A JP 2009127100 A JP2009127100 A JP 2009127100A JP 2009127100 A JP2009127100 A JP 2009127100A JP 2010273572 A JP2010273572 A JP 2010273572A
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bacteria
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Hiroyuki Ogawa
廣幸 小川
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MICROBIO CORP
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a bacterium examination method and a bacterium examination tool which each is easy in examination and high in the precision of bacterium examination. <P>SOLUTION: The bacterium examination method comprises adding a sample 1 to a liquid container 11 receiving a liquid culture medium 21 capable of culturing a prescribed bacterium to be detected, thereby mixing the liquid culture medium 21 with the sample 1, impregnating and culturing the mixture liquid 24 in a water-absorbing sheet 23 put on an agar culture medium 22 received in the culture container 12, and then applying light to the culture container 12 from the lower side of the culture container to detect the prescribed bacterium of examination target on the basis of a profile view due to the light transmitting the water-absorbing sheet 23. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、細菌検査方法および細菌検査用具に関する。   The present invention relates to a bacteria testing method and a bacteria testing tool.

従来、寒天培地は、微生物学や植物学の分野で、細菌や細胞を培養するために使用されている。寒天培地は、固形培地であり、通常、水に約1.5%の濃度の寒天を加えて固化させることにより作製される。   Conventionally, an agar medium is used for culturing bacteria and cells in the fields of microbiology and botany. The agar medium is a solid medium, and is usually prepared by adding agar with a concentration of about 1.5% to water and solidifying it.

一般的な細菌検査では、寒天平板培地を用い、試料0.1ミリリットルを培地の表面に塗抹して室温あるいはインキュベータなどで一定期間培養している(塗沫培養法)。しかし、検査対象の試料が1ミリリットル程度の量のときには、通常、寒天培地の表面には塗抹することができない。このため、塗抹する代わりに、検査対象の試料と、溶解後40℃ほどに温度を下げた寒天培地とをシャーレに入れて混ぜ合わせ、固化後、培養する方法(混釈培養法)が行われている(例えば、特許文献1または2参照)。   In a general bacterial test, an agar plate medium is used, and 0.1 ml of a sample is smeared on the surface of the medium and cultured at room temperature or in an incubator for a certain period (a smear culture method). However, when the sample to be inspected is about 1 milliliter, it cannot usually be smeared on the surface of the agar medium. For this reason, instead of smearing, the sample to be examined and the agar medium whose temperature has been lowered to about 40 ° C after dissolution are put in a petri dish, mixed, solidified, and cultured (pour culture method). (For example, refer to Patent Document 1 or 2).

試料中に細菌が存在する場合、これを混釈培養法などで培養すると、細菌が分裂増殖を繰り返して目視で確認できるコロニーを形成するため、このコロニーの有無により、細菌が試料中に存在していたかどうかを判定できる。なお、この判定の際、寒天培地を通過する光による投影像に基づいて、細菌を正確かつ迅速に検出することができる投影検出法が本発明者等により提案されている(例えば、特許文献3参照)。   When bacteria are present in the sample, if they are cultured by a pour culture method, etc., the bacteria form a colony that can be visually confirmed by repeated division and growth. It can be determined whether it was. In this determination, the present inventors have proposed a projection detection method capable of accurately and quickly detecting bacteria based on a projection image by light passing through an agar medium (for example, Patent Document 3). reference).

特開2008−187972号公報JP 2008-187972 A 特開2008−131928号公報JP 2008-131928 A 特開2003−85533号公報JP 2003-85533 A

特許文献1や特許文献2に記載のような混釈培養法を行う場合、溶解した寒天培地の熱により、試料中の細菌がダメージを受けることがあり、細菌を検出できないことがあるという課題があった。また、検出目的の細菌を培養するために、寒天培地を細菌の種類に応じた組成で作製しなければならず、様々な種類の細菌に対応させて寒天培地を作製するのは煩雑であるという課題もあった。   When performing the pour culture method as described in Patent Document 1 or Patent Document 2, there is a problem that the bacteria in the sample may be damaged by the heat of the dissolved agar medium and the bacteria may not be detected. there were. In addition, in order to culture bacteria for detection purposes, it is necessary to prepare an agar medium with a composition according to the type of bacteria, and it is complicated to prepare an agar medium corresponding to various types of bacteria. There were also challenges.

本発明は、このような課題に着目してなされたもので、検査が容易で、細菌の検出精度が高い細菌検査方法および細菌検査用具を提供することを目的としている。   The present invention has been made paying attention to such a problem, and an object thereof is to provide a bacteria testing method and a bacteria testing tool that are easy to test and have high bacteria detection accuracy.

上記目的を達成するために、本発明に係る細菌検査方法は、細菌を培養可能な液体培地に試料を加え、その試料を含む液体培地をゲル培地の上に載置された吸水シートに浸透させて培養した後、吸水シート付近の細菌を検査することを、特徴とする。   In order to achieve the above object, a method for testing bacteria according to the present invention includes adding a sample to a liquid medium in which bacteria can be cultured, and allowing the liquid medium containing the sample to permeate a water-absorbing sheet placed on the gel medium. And then inspecting bacteria near the water-absorbing sheet.

本発明に係る細菌検査方法では、試料に検査の対象となる所定の細菌が存在するときには、その細菌を培養可能な液体の液体培地と試料との混合液を培養することにより、その細菌が分裂増殖を繰り返してコロニーを形成するため、このコロニーの有無により細菌が試料中に存在していたかどうかを判定することができる。このとき、液体の液体培地と試料との混合液が浸透した吸水シートに、ゲル培地から水分が供給されるため、長時間培養してもシートが乾燥するのを防ぐことができる。   In the method for inspecting bacteria according to the present invention, when a predetermined bacterium to be inspected exists in a sample, the bacterium is divided by culturing a mixed liquid of a liquid medium that can cultivate the bacterium and the sample. Since the growth is repeated to form a colony, it is possible to determine whether or not bacteria are present in the sample based on the presence or absence of the colony. At this time, since water is supplied from the gel medium to the water-absorbing sheet into which the liquid mixture of the liquid medium and the sample has permeated, it is possible to prevent the sheet from drying even if cultured for a long time.

また、試料を、熱をもった溶解した寒天培地に混ぜる必要がないため、試料中の細菌に熱によるダメージを与えることなく検査を行うことができ、細菌の検出精度が高い。本発明に係る細菌検査方法で、より検査精度を高めるために、シートは滅菌済みであることが好ましい。   In addition, since it is not necessary to mix the sample with a dissolved agar medium with heat, the test can be performed without damaging the bacteria in the sample by heat, and the detection accuracy of the bacteria is high. In order to further improve the test accuracy in the method for testing bacteria according to the present invention, the sheet is preferably sterilized.

ゲル培地は、載置されたシートに水分を補給可能であればいかなるものから成っていてもよい。ゲル培地は、液体培地でも固体培地でもよく、例えば、寒天のみの組成から成る寒天培地から成っていてもよい。本発明に係る細菌検査方法では、ゲル培地を検査の対象となる細菌の種類に応じた組成で作製する必要がないため、様々な種類の細菌に対応して寒天培地を作製する場合と比べて、容易に検査を行うことができる。   The gel medium may be composed of any material as long as moisture can be supplied to the placed sheet. The gel medium may be a liquid medium or a solid medium. For example, the gel medium may be an agar medium composed of only agar. In the method for testing bacteria according to the present invention, it is not necessary to prepare a gel medium with a composition according to the type of bacteria to be tested, so compared to the case of preparing an agar medium corresponding to various types of bacteria. Can be easily inspected.

本発明に係る細菌検査方法で、前記ゲル培地は培養容器に収容され、前記培養容器、前記ゲル培地、前記液体培地および前記吸水シートは透光性を有し、前記培養容器、前記ゲル培地、前記液体培地および前記吸水シートを透過する光による細菌の投影像に基づいて細菌を検査することが好ましい。この場合、シート中で細菌が増殖してコロニーを形成し、そのシートが投影像を映すスクリーンの役目をするため、コロニーを明瞭かつ確実に観察することができ、より高い精度で細菌を検出することができる。ゲル培地は、透光性であることが好ましい。この場合、ゲル培地のシートとは反対側から光をあてることにより、シートを透過する光による投影像を容易に得ることができる。   In the method for testing bacteria according to the present invention, the gel medium is accommodated in a culture container, and the culture container, the gel medium, the liquid medium, and the water absorbing sheet have translucency, the culture container, the gel medium, It is preferable to inspect the bacteria based on a projected image of the bacteria by light transmitted through the liquid medium and the water absorbing sheet. In this case, bacteria grow in the sheet to form a colony, and the sheet acts as a screen for projecting a projected image, so that the colony can be clearly and reliably observed, and the bacteria are detected with higher accuracy. be able to. The gel medium is preferably translucent. In this case, the projection image by the light which permeate | transmits a sheet | seat can be easily obtained by irradiating light from the opposite side to the sheet | seat of a gel culture medium.

本発明に係る細菌検査方法で、前記シートは、デンプン、カオリン、合成シリカ、ガラス、微結晶セルロース、イオン交換セルロースまたはケイ酸アルミニウムにより形成されたろ紙から成っていてもよい。この場合、吸水性が良く、透明度が高いシートを形成することができる。また、水分を含んだときに極端な酸性や塩基性を示さないシートを形成することができる。このようなシートを使用することにより、細菌にダメージを与えることなく、高い精度で細菌を検出することができる。   In the method for testing bacteria according to the present invention, the sheet may be made of a filter paper formed of starch, kaolin, synthetic silica, glass, microcrystalline cellulose, ion exchange cellulose, or aluminum silicate. In this case, a sheet having good water absorption and high transparency can be formed. In addition, a sheet that does not exhibit extreme acidity or basicity when it contains moisture can be formed. By using such a sheet, bacteria can be detected with high accuracy without damaging the bacteria.

本発明に係る細菌検査用具は、試料内の細菌を検査するための細菌検査用具であって、細菌を培養可能な液体培地を収容した液体容器と、ゲル培地を収容した培養容器と、前記培養容器の内部で前記ゲル培地の上に載置される吸水シートとを、有することを特徴とする。   A bacteria testing tool according to the present invention is a bacterial testing tool for testing bacteria in a sample, a liquid container containing a liquid medium capable of culturing bacteria, a culture container containing a gel medium, and the culture And a water-absorbing sheet placed on the gel medium inside the container.

本発明に係る細菌検査用具は、本発明に係る細菌検査方法で好適に使用される。本発明に係る細菌検査用具は、液体容器の内部に試料を加えて液体培地と混合し、その混合液を培養容器の吸水シートに浸透させて培養する。試料に検査の対象となる所定の細菌が存在するときには、その細菌が分裂増殖を繰り返してコロニーを形成するため、このコロニーの有無により細菌が試料中に存在していたかどうかを判定することができる。   The bacteria test tool according to the present invention is suitably used in the bacteria test method according to the present invention. The bacterial test tool according to the present invention adds a sample to the inside of a liquid container, mixes it with a liquid medium, and infiltrates the mixed liquid into the water-absorbing sheet of the culture container and cultures it. When a certain bacterium to be examined exists in the sample, the bacterium repeats division and growth to form a colony. Therefore, whether or not the bacterium was present in the sample can be determined by the presence or absence of this colony. .

本発明に係る細菌検査用具は、培養中、液体培地と試料との混合液が浸透した吸水シートに、下の液体または固体の培地から水分が供給されるため、長時間培養してもシートが乾燥するのを防ぐことができる。また、試料を、熱をもった溶解した寒天培地に混ぜる必要がないため、試料中の細菌に熱によるダメージを与えることなく検査を行うことができ、細菌の検出精度が高い。本発明に係る細菌検査用具では、より検査精度を高めるために、シートは滅菌済みであることが好ましい。   In the bacterial test tool according to the present invention, since water is supplied from the lower liquid or solid medium to the water-absorbing sheet in which the mixed liquid of the liquid medium and the sample has permeated during the culture, the sheet remains even after culturing for a long time. Drying can be prevented. In addition, since it is not necessary to mix the sample with a dissolved agar medium with heat, the test can be performed without damaging the bacteria in the sample by heat, and the detection accuracy of the bacteria is high. In the bacterial test tool according to the present invention, the sheet is preferably sterilized in order to further improve the test accuracy.

培養容器内の培地は、載置されたシートに水分を補給可能であればいかなるものから成っていてもよく、例えば、寒天のみの組成から成る寒天培地から成っていてもよい。本発明に係る細菌検査用具では、培養容器内の培地を検査の対象となる細菌の種類に応じた組成で作製する必要がないため、様々な種類の細菌に対応して寒天培地を作製する場合と比べて、容易に検査を行うことができる。   The culture medium in the culture vessel may be composed of any material as long as it can replenish water on the placed sheet. For example, the culture medium may be composed of an agar medium composed of only agar. In the bacterial test tool according to the present invention, since it is not necessary to prepare the culture medium in the culture container with a composition according to the type of bacteria to be tested, when preparing an agar medium corresponding to various types of bacteria Compared to the above, inspection can be performed easily.

本発明に係る細菌検査用具で、前記培養容器、前記ゲル培地、前記液体培地および前記吸水シートは透光性を有することが好ましい。特に、前記シートは、デンプン、カオリン、合成シリカ、ガラス、微結晶セルロース、イオン交換セルロースまたはケイ酸アルミニウムにより形成されたろ紙から成ることが好ましい。この場合、吸水性が良く、透明度が高いシートを形成することができる。また、水分を含んだときに極端な酸性や塩基性を示さないシートを形成することができる。このようなシートを使用することにより、細菌にダメージを与えることなく、高い精度で細菌を検出することができる。また、培養後にシートを透過する光による投影像に基づいて、所定の細菌の検出を行うことができる。このとき、シート中で細菌が増殖してコロニーを形成し、そのシートが投影像を映すスクリーンの役目をするため、コロニーを明瞭かつ確実に観察することができ、より高い精度で細菌を検出することができる。培養容器内の培地は、透光性であることが好ましい。この場合、培地のシートとは反対側から光をあてることにより、シートを透過する光による投影像を容易に得ることができる。   In the bacterial test tool according to the present invention, it is preferable that the culture container, the gel medium, the liquid medium, and the water absorbing sheet have translucency. In particular, the sheet is preferably made of filter paper formed of starch, kaolin, synthetic silica, glass, microcrystalline cellulose, ion exchange cellulose or aluminum silicate. In this case, a sheet having good water absorption and high transparency can be formed. In addition, a sheet that does not exhibit extreme acidity or basicity when it contains moisture can be formed. By using such a sheet, bacteria can be detected with high accuracy without damaging the bacteria. Moreover, based on the projection image by the light which permeate | transmits a sheet | seat after culture | cultivation, a predetermined | prescribed bacteria can be detected. At this time, bacteria grow in the sheet to form a colony, and the sheet acts as a screen for projecting a projected image, so that the colony can be clearly and reliably observed, and the bacteria are detected with higher accuracy. be able to. The medium in the culture container is preferably translucent. In this case, the projection image by the light which permeate | transmits a sheet | seat can be easily obtained by irradiating light from the opposite side to the sheet | seat of a culture medium.

本発明によれば、検査が容易で、細菌の検出精度が高い細菌検査方法および細菌検査用具を提供することができる。   ADVANTAGE OF THE INVENTION According to this invention, the test | inspection method and bacteria test | inspection tool which are easy to test | inspect and have high detection accuracy of bacteria can be provided.

本発明の実施の形態の細菌検査用具を示す斜視図である。It is a perspective view which shows the bacteria test | inspection tool of embodiment of this invention. 本発明の実施の形態の細菌検査方法を示す斜視図である。It is a perspective view which shows the bacteria test | inspection method of embodiment of this invention.

以下、図面に基づき本発明の実施の形態について説明する。
図1および図2は、本発明の実施の形態の細菌検査方法および細菌検査用具を示している。
図1に示すように、本発明の実施の形態の細菌検査用具は、試料1内に所定の細菌が存在するかどうかを調べるための細菌検査セットであって、液体容器11と培養容器12とを有している。
Hereinafter, embodiments of the present invention will be described with reference to the drawings.
1 and 2 show a bacteria testing method and a bacteria testing tool according to an embodiment of the present invention.
As shown in FIG. 1, the bacteria testing tool according to the embodiment of the present invention is a bacteria testing set for examining whether or not predetermined bacteria are present in a sample 1, and includes a liquid container 11, a culture container 12, have.

図1に示すように、液体容器11は、蓋11aを有する試験管形状の容器から成り、あらかじめ内部に液体培地21が収容されている。液体培地21は、透光性で、検出しようとする所定の細菌を培養可能な培地から成っている。液体培地21は、例えば、食中毒を引き起こすサルモネラ菌を検出する場合には、サルモネラ菌を培養可能な培地から成る。液体容器11は、内部に試料1を加えて、試料1と液体培地21とを混合可能になっている。   As shown in FIG. 1, the liquid container 11 is a test tube-shaped container having a lid 11a, and a liquid medium 21 is accommodated therein in advance. The liquid medium 21 is translucent and is made of a medium capable of culturing predetermined bacteria to be detected. For example, when detecting Salmonella causing food poisoning, the liquid medium 21 is composed of a medium capable of culturing Salmonella. In the liquid container 11, the sample 1 can be added to mix the sample 1 and the liquid medium 21.

図1に示すように、培養容器12は、蓋12aを有する透明なシャーレから成り、内部に寒天培地22が収容され、その寒天培地22の上に吸水シート23が載置されている。寒天培地22は、透光性で、載置された吸水シート23に水分を補給可能であればいかなるものから成っていてもよく、例えば、1リットル当たり10gのペプトンを含む一般的な寒天培地から成る。吸水シート23は、滅菌済みのろ紙から成り、吸水性が良く、透明度が高い。また、吸水シート23は、試料1と液体培地21との混合液24を浸透させたとき透光性を有し、水分を含んだときに極端な酸性や塩基性を示さないものから成っている。吸水シート23は、例えば、デンプン、カオリン、合成シリカ、ガラス、微結晶セルロース、イオン交換セルロースまたはケイ酸アルミニウムにより形成されたろ紙から成る。   As shown in FIG. 1, the culture container 12 is made of a transparent petri dish having a lid 12 a, in which an agar medium 22 is accommodated, and a water absorbing sheet 23 is placed on the agar medium 22. The agar medium 22 may be made of any material as long as it is translucent and can replenish water on the placed water-absorbing sheet 23. For example, the agar medium 22 is composed of a general agar medium containing 10 g of peptone per liter. Become. The water absorbing sheet 23 is made of sterilized filter paper, has good water absorption, and has high transparency. Further, the water absorbent sheet 23 is made of a material that is translucent when the mixed solution 24 of the sample 1 and the liquid medium 21 is infiltrated, and that does not exhibit extreme acidity or basicity when containing water. . The water absorbing sheet 23 is made of, for example, a filter paper made of starch, kaolin, synthetic silica, glass, microcrystalline cellulose, ion exchange cellulose, or aluminum silicate.

本発明の実施の形態の細菌検査用具は、本発明の実施の形態の細菌検査方法で好適に使用される。本発明の実施の形態の細菌検査方法では、まず、図2(a)に示すように、液体容器11の内部に、ピペット31等を利用して試料1(サンプル)を加えて液体培地21と混合する。加える試料1の量は、例えば1ミリリットルである。次に、図2(b)に示すように、その混合液24を、ピペット31等を利用して寒天培地22の上に載置された吸水シート23に滴下して浸透させ、培養する。   The bacteria testing tool of the embodiment of the present invention is suitably used in the bacteria testing method of the embodiment of the present invention. In the bacteria testing method according to the embodiment of the present invention, first, as shown in FIG. 2A, the sample 1 (sample) is added to the inside of the liquid container 11 by using a pipette 31 or the like, Mix. The amount of sample 1 to be added is, for example, 1 milliliter. Next, as shown in FIG.2 (b), the liquid mixture 24 is dripped at the water absorption sheet | seat 23 mounted on the agar medium 22 using the pipette 31, etc., and is made to permeate | transmit and culture | cultivate.

培養後、培養容器12の下、すなわち寒天培地22の吸水シート23とは反対側から光をあて、培養容器12、寒天培地22、液体培地21および吸水シート23を透過する光による細菌の投影像により細菌の検出を行う。試料1に検査の対象となる所定の細菌が存在するときには、その細菌が分裂増殖を繰り返して吸水シート23中にコロニーを形成するため、このコロニーの有無により細菌が試料中に存在していたかどうかを判定することができ、検査対象の所定の細菌を検出することができる。培養容器12の下から光をあてると、その光が透光性の寒天培地22および吸水シート23を透過し、吸水シート23に投影像が映し出される。このように、吸水シート23が投影像を映すスクリーンの役目をするため、吸水シート23中に形成されたコロニーを明瞭かつ確実に観察することができ、より高い精度で細菌を検出することができる。   After culturing, a projection image of bacteria by light passing through the culture container 12, the agar medium 22, the liquid medium 21, and the water absorption sheet 23 under the culture container 12, that is, from the side opposite to the water absorption sheet 23 of the agar medium 22. To detect bacteria. When there is a predetermined bacterium to be examined in the sample 1, the bacterium repeats division and growth to form a colony in the water-absorbent sheet 23. Therefore, whether or not the bacterium is present in the sample due to the presence or absence of this colony. And a predetermined bacterium to be inspected can be detected. When light is applied from below the culture vessel 12, the light passes through the translucent agar medium 22 and the water absorbing sheet 23, and a projected image is displayed on the water absorbing sheet 23. Thus, since the water absorbing sheet 23 serves as a screen for projecting a projection image, the colonies formed in the water absorbing sheet 23 can be clearly and reliably observed, and bacteria can be detected with higher accuracy. .

本発明の実施の形態の細菌検査方法および細菌検査用具によれば、培養中、液体培地21と試料1との混合液24が浸透した吸水シート23に、下の寒天培地22から水分が供給されるため、長時間培養しても吸水シート23が乾燥するのを防ぐことができる。また、試料1を、熱をもった溶解した寒天培地22に混ぜる必要がないため、試料1中の細菌に熱によるダメージを与えることなく検査を行うことができ、細菌の検出精度が高い。   According to the bacteria testing method and the bacteria testing tool of the embodiment of the present invention, moisture is supplied from the lower agar medium 22 to the water-absorbing sheet 23 into which the liquid mixture 21 of the liquid medium 21 and the sample 1 has permeated. Therefore, even if it culture | cultivates for a long time, it can prevent that the water absorbing sheet 23 dries. Moreover, since it is not necessary to mix the sample 1 with the melted agar medium 22 with heat, it can test | inspect without damaging the bacteria in the sample 1 by heat, and the detection accuracy of bacteria is high.

本発明の実施の形態の細菌検査方法および細菌検査用具では、培養容器12内の寒天培地22を検査の対象となる細菌の種類に応じた組成で作製する必要がないため、様々な種類の細菌に対応して寒天培地22を作製する場合と比べて、容易に検査を行うことができる。本発明の実施の形態の細菌検査方法および細菌検査用具によれば、サルモネラ菌だけでなく、カビやボツリヌス菌など、好気的条件下または嫌気的条件下で様々な細菌を高精度で検出することができる。   In the bacteria testing method and the bacteria testing tool according to the embodiment of the present invention, it is not necessary to prepare the agar medium 22 in the culture container 12 with a composition according to the type of bacteria to be tested. Compared with the case where the agar medium 22 is produced corresponding to the above, the inspection can be easily performed. According to the bacteria testing method and the bacteria testing tool of the embodiment of the present invention, various bacteria can be detected with high accuracy not only in Salmonella but also in aerobic or anaerobic conditions such as mold and botulinum. Can do.

1 試料
11 液体容器
12 培養容器
21 液体培地
22 寒天培地
23 吸水シート
24 混合液
31 ピペット
1 Sample 11 Liquid Container 12 Culture Container 21 Liquid Medium 22 Agar Medium 23 Water Absorbent Sheet 24 Mixed Solution 31 Pipette

Claims (3)

試料内の細菌を検査する細菌検査方法であって、細菌を培養可能な液体培地に試料を加え、その試料を含む液体培地をゲル培地の上に載置された吸水シートに浸透させて培養した後、吸水シート付近の細菌を検査することを、特徴とする細菌検査方法。   A method for inspecting bacteria in a sample, wherein the sample is added to a liquid medium in which bacteria can be cultured, and the liquid medium containing the sample is permeated into a water-absorbing sheet placed on the gel medium and cultured. A method for inspecting bacteria, characterized by inspecting bacteria near the water-absorbing sheet. 前記ゲル培地は培養容器に収容され、前記培養容器、前記ゲル培地、前記液体培地および前記吸水シートは透光性を有し、前記培養容器、前記ゲル培地、前記液体培地および前記吸水シートを透過する光による細菌の投影像に基づいて細菌を検査することを、特徴とする請求項1記載の細菌検査方法。   The gel medium is accommodated in a culture container, and the culture container, the gel medium, the liquid medium, and the water absorbing sheet have translucency, and pass through the culture container, the gel medium, the liquid medium, and the water absorbing sheet. 2. The method for inspecting bacteria according to claim 1, wherein the bacteria are inspected based on a projected image of the bacteria by the light to be emitted. 試料内の細菌を検査するための細菌検査用具であって、
細菌を培養可能な液体培地を収容した液体容器と、
ゲル培地を収容した培養容器と、
前記培養容器の内部で前記ゲル培地の上に載置される吸水シートとを、
有することを特徴とする細菌検査用具。

A bacterial test tool for testing bacteria in a sample,
A liquid container containing a liquid medium capable of culturing bacteria;
A culture vessel containing a gel medium;
A water absorbing sheet placed on the gel medium inside the culture vessel,
Bacteria testing tool characterized by having.

JP2009127100A 2009-05-27 2009-05-27 Bacterium examination method and bacterium examination tool Pending JP2010273572A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023133714A1 (en) * 2022-01-12 2023-07-20 广州工商学院 Fungus quantity measurement device and measurement method for testing yogurt quality

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023133714A1 (en) * 2022-01-12 2023-07-20 广州工商学院 Fungus quantity measurement device and measurement method for testing yogurt quality

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