JP2010209039A - Interleukin-6 production inhibitor and skin care preparation or cosmetic product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new interleukin-6 production inhibitor having high safety, and a skin care preparation or a cosmetic product obtained by using the inhibitor and having high antiinflammatory action or pachyderma suppressing action. <P>SOLUTION: There are provided the interleukin-6 production inhibitor containing a component derived from peanut sprout as an active component, a pachyderma suppressing skin care preparation or cosmetic product or an antiinflammatory skin care preparation or cosmetic product containing the interleukin-6 production inhibitor. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a novel interleukin 6 production inhibitor and a skin external preparation or cosmetic containing the same.

  Cytokines, including interleukin 6 (IL6), are produced from cells to maintain tissue homeostasis, but on the other hand, continuous exposure to ultraviolet rays (sunlight) over a long period of time, and external / internal It is known that it is excessively produced by continuous stimulation of the skin and damages the skin tissue. In particular, excessive production of IL6 is deeply involved in the proliferation of keratinocytes, collagen and proteoglycan production from fibroblasts and promotion of collagenase secretion, and is considered to cause skin damage such as skin inflammation and skin thickening. . Various agents that suppress excessive production of IL6 related to these skin injuries have been proposed (for example, Patent Documents 1 and 2).

  On the other hand, peanut bean sprout-derived components are known for their ability to suppress melanin production (for example, Patent Document 3), but nothing is known about their effects on IL6.

JP 2007-269636 A JP 2007-63154 A JP 2007-238579 A

  It is an object of the present invention to provide a novel interleukin 6 production inhibitor having good safety, and a skin external preparation or cosmetic using the same, which has a good anti-inflammatory action or skin thickening inhibitory action. .

As a result of various studies conducted by the present inventors to solve the above-mentioned problems, it has been found that a component derived from peanut sprouts has a high IL6 production inhibitory action, and the present invention has been completed based on this finding.
That is, in order to solve the above-mentioned problems, the present invention provides an interleukin 6 production inhibitor containing a peanut sprouts-derived ingredient as an active ingredient; and a skin external preparation or cosmetic for suppressing skin thickening containing the interleukin 6 production inhibitor. And an anti-inflammatory skin external preparation or cosmetic.

  ADVANTAGE OF THE INVENTION According to this invention, the novel interleukin 6 production inhibitor with favorable safety | security and the skin external preparation or cosmetics using the anti-inflammatory effect or skin thickening inhibitory effect using the same can be provided. .

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The present invention relates to an IL6 production inhibitor comprising as an active ingredient a sprout-derived component of peanuts belonging to the legume family (Arachis hypogaea L .; hereinafter simply referred to as “peanuts”). As for the peanut used in the present invention, its production area and cultivation method are not particularly limited, and the method for preparing the peanut sprouts-derived component used in the present invention is not particularly limited. In this specification, “peanut bean sprout” means that peanut seeds are immersed in water at about 15 ° C. to 25 ° C. and germinated in the absence of light for about 2 to 7 days, and then further cultivated, It shall mean a hypocotyl of about 40-100 mm with roots growing and seed parts almost digested.

  An example of a component derived from peanut bean sprout used in the present invention is a powder obtained by dry pulverizing, crushing, or the like. This powder can be used as it is as an IL6 production inhibitor. The powder may be used after post-treatment such as sterilization and sieving.

  Another example of the component derived from peanut bean sprout used in the present invention is an extract obtained by subjecting the powder obtained by the above method or the like to a peanut bean sprout using a solvent (hereinafter simply referred to as “peanut bean sprout extract”). It is noted). The peanut bean sprout extract can be used as it is as an IL6 production inhibitor, or can be used after further post-treatment such as decolorization and deodorization as necessary. Moreover, only a highly effective fraction can also be used by separation operation. Furthermore, the solvent can be distilled off to form a solid or powder (hereinafter simply referred to as “peanut bean sprout extract”). Examples of the extraction solvent used for the extraction treatment include water, lower monohydric alcohol (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol (glycerin, propylene). Glycol, 1,3-butylene glycol, etc.), lower esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether) Etc.), acetonitrile and the like, and one or more of them can be used.

  As an example of a preferable extraction method, water-containing ethyl alcohol or water-containing 1,3-butylene glycol having a concentration of 0 to 100 vol% is used, and after extraction at room temperature or for 1 to 10 days after heating, filtration is performed to obtain A method of further purifying the obtained filtrate by column fractionation, molecular weight fractionation or the like using a hydrophobic resin, an ion exchange resin or the like can be mentioned.

The present invention also relates to a skin external preparation or cosmetic containing the IL6 production inhibitor of the present invention, that is, a component derived from peanut sprouts as an active ingredient. The component derived from peanut sprouts has an excellent effect of suppressing excessive production of IL6 due to ultraviolet irradiation without impairing the amount of IL6 in a normal state when not irradiated with ultraviolet light. As described above, overproduction of IL6 contributes to skin disorders such as skin inflammation and skin thickening. By using the IL6 production inhibitor of the present invention, it is possible to provide a skin external preparation or cosmetic that prevents or ameliorates such skin disorders.
The blending amount of the component derived from peanut bean sprout used in the present invention into the external preparation for skin or cosmetic is preferably 0.001 to 5% by mass (hereinafter simply indicated as “%”), more preferably 0. 0.01 to 3%. Within this range, peanut bean sprout-derived components can be stably blended and can exhibit high medicinal effects. Moreover, when using an extract, it is preferable that content of the dry part which is a solute is in the said range.

  The skin external preparation or cosmetic of the present invention is prepared by blending peanut bean sprout-derived components into various forms of bases used in normal skin external preparations or cosmetics in accordance with conventional methods using an IL6 production inhibitor. be able to. In addition, the effects of the present invention are combined with other medicinal ingredients, for example, whitening agents, antioxidants, other anti-inflammatory agents, cell activators, and ultraviolet ray inhibitors, as long as the effects of the present invention are not impaired. Further enhancement or other effects can be added.

  Moreover, arbitrary components other than the IL6 production inhibitor of this invention can be mix | blended with the skin external preparation or cosmetics of this invention. Examples of such components include, but are not limited to, physiologically active substances such as amino acids, lipids, sugars, hormones, enzymes, and nucleic acids. In addition, components that are usually used in the production of cosmetics, quasi-drugs, skin external preparations and the like, for example, water (purified water, hot spring water, deep water, etc.), oil agents, Surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, fragrance, deodorant, salt, pH adjuster, refreshing agent, plant / animal -Extracts derived from microorganisms, blood circulation promoters, astringents, antiseborrheic agents, chelating agents, keratolytic agents, enzymes, hormones, vitamins and the like can be used as necessary.

  The present invention may be in the form of a quasi-drug or a pharmaceutical in addition to a skin external preparation or cosmetic. Moreover, any form, such as powder, solid, an emulsion, and a liquid, may be sufficient. More specifically, the external preparation for skin and cosmetics of the present invention are powders such as powder and powder foundation; solids such as soap and lipstick; emulsions such as cream, emulsion and cream foundation; Any of various forms of embodiments, such as a liquid;

The present invention will be described more specifically with reference to the following examples. However, the scope of the present invention is not limited to the following examples.
[Example 1: Preparation example of components derived from peanut sprouts]
A seed of peanut (Arachis hypogaea L.) was immersed in water at 15 ° C. to 25 ° C. and germinated under non-light for 5 days. " Furthermore, it was freeze-dried for storage, and peanut bean sprout powder was obtained through a pulverization process.
To 100 g of the pulverized product thus obtained, 1,000 mL of 50 vol% hydrous ethyl alcohol was added, and extraction treatment was performed at 50 ° C. for 2 hours. After cooling, the mixture was filtered under pressure to obtain an extract adjusted to have an ethyl alcohol concentration of 25 vol%. The dry matter concentration of this extract was 2.0% by mass.
This extract was used in the following IL6 production inhibition test.

[Example 2: IL6 production inhibition test]
The effect of inhibiting interleukin 6 production during UV irradiation in keratinocytes was confirmed by the following method.
Human normal skin keratinocytes (Kurabo) were cultured using KGM2 medium (Kurabo), seeded in a 24-well plate at a density of 1 × 10 ⁴ / cm ² , and cultured to a subconfluent state. To this cultured cell, the extract prepared above was added to 2.0, 1.0, and 0.2% as final concentrations in the medium, respectively. Three hours later, after washing twice with PBS, Hank's solution was added twice and irradiated with 50 mJ of ultraviolet light (UV-B). After removing the Hanks solution, the medium was added, and each sample solution was added again at the same rate as above. After 24 hours, the medium was collected. The collected medium was subjected to centrifugal filtration to remove cell components and the like. For each of the separated media, the average concentration of IL6 (nano g / mL) was measured using a measurement kit (Interleukin-6 [(h) IL-6] Human, ELISA; manufactured by Biotrak System). In addition, it measured similarly about the cell which is not irradiated with an ultraviolet-ray. The results are shown in the table below.
In the table below, “control” is the measurement result of the medium without the sample solution, “UV +” is the measurement result of the medium irradiated with ultraviolet light, and “UV−” is not irradiated with ultraviolet light. It is a measurement result of a culture medium. The IL6 production inhibition rate (%) is a value calculated by the following formula (1) using the average concentration value of IL6 production at the time of “UV +”.
(1)
IL6 production inhibition rate = {1− (value of each test average concentration / value of control)} × 100

  From the above results, the peanut bean sprouts natural product-derived component does not inhibit the production of IL6 when irradiated with ultraviolet rays without inhibiting the production of IL6 when not irradiated with ultraviolet rays, that is, without impairing the normal amount of IL6. It can be understood that there is an excellent effect.

[Example 3: Preparation of lotion]
A lotion having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (7) are mixed and dissolved.
B. The following components (8) to (11) are mixed and dissolved.
C. B was added to A and mixed to obtain a lotion.
(Ingredient)%
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(4) Peanut bean sprout extract * 1 0.1
(5) Glycerin 3.0
(6) 1,3-butylene glycol 8.0
(7) Purified water remaining amount (8) Ethyl alcohol 10.0
(9) Perfume Appropriate amount (10) Preservative Appropriate amount (11) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1 Extract prepared in Example 1

  The prepared lotion is a lotion that has a fresh feeling of use, is excellent in moisture, has a skin thickening-inhibiting action and an anti-inflammatory action, and is smooth and glossy. It was.

[Example 4: Preparation of emulsion]
An emulsion having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (10) are dissolved by heating and maintained at 70 ° C.
B. The following components (11) to (15) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (16) is further added and mixed.
D. C is cooled, the following components (17) and (18) are added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Triethanolamine 0.5
(12) 1,3-butylene glycol 15.0
(13) Glycerin 3.0
(14) Polyethylene glycol 6000 0.5
(15) Purified water Remaining amount (16) Carboxyvinyl polymer 1% solution 8.0
(17) Peanut sprouts extract * 1 2.0
(18) Perfume appropriate amount * 1 Extract prepared in Example 1

  The prepared emulsion has a fresh and smooth feeling of use, is excellent in moisture and emollient feeling on the skin, exhibits skin thickening suppression and anti-inflammatory effects, prepares the texture, and also makes the skin beautiful and elastic. It was an emulsion that can.

[Example 5: Preparation example of cream]
The following compositional claims were prepared in the following manner.
(Manufacturing method)
A. The following components (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. The following components (14) to (18) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (19) is further added and mixed.
D. C is cooled, and the following components (20) and (21) are added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester * 1 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(16) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Purified water remaining amount (19) 1% aqueous solution of carboxyvinyl polymer 5.0
(20) Peanut sprouts extract * 2 5.0
(21) Fragrance appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2 Extract prepared in Example 1

  The prepared cream has a rich and smooth feel, excellent emollient skin, anti-thickening and anti-inflammatory effects, smoothing texture, and glossy, beautiful skin It was a possible cream.

[Example 6: Preparation example of pack]
A pack having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (5) are dissolved by heating.
B. The following components (6) to (10) are mixed and dissolved.
C. After cooling A, B and the following (11) were added and mixed to obtain a pack.
(Ingredient)%
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Purified water remaining amount (6) perfume appropriate amount (7) preservative appropriate amount (8) glyceryl tri-2-ethylhexanoate 0.1
(9) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(10) Ethyl alcohol 13.0
(11) Peanut sprouts extract * 1 0.1
* 1 Extract prepared in Example 1

  The prepared pack has good spread, gives the skin an appropriate tension, does not stick to the skin after peeling off the pack, exhibits anti-thickening action and anti-inflammatory action, and has high moisturizing and stickiness. It was a pack that came out.

[Example 7: Preparation example of cream foundation]
A cream foundation having the following composition was prepared by the following method.
(Manufacturing method)
A: The following components (8) to (13) are mixed.
B: The following components (14) to (19) are mixed.
C: The following components (1) to (7) are added to A and mixed and dispersed.
D: Add B to C and mix.
E: The following components (20) and (21) were added to D to obtain a cream foundation.
(Ingredient)%
(1) Sericite 8.0
(2) Titanium oxide 10.0
(3) Bengala 1.0
(4) Yellow iron oxide 2.2
(5) Mica titanium 2.0
(6) Black iron oxide 0.1
(7) Organic metamorphic bentonite * 1 0.5
(8) Long chain alkyl-containing polyoxyalkylene modified organopolysiloxane * 2 2.0
(9) Polyoxyalkylene-modified organopolysiloxane * 3 2.0
(10) Decamethylcyclopentasiloxane 12.0
(11) Octamethylcyclotetrasiloxane 10.0
(12) Trimethylsiloxysilicic acid silicone solution * 4 3.0
(13) Glyceryl tri-2-ethylhexanoate 5.0
(14) 1,3-butylene glycol 5.0
(15) Diglycerin 2.0
(16) Glycerin 2.0
(17) Xanthan gum 0.2
(18) Sodium chloride 0.2
(19) Purified water remaining amount (20) Peanut bean sprout extract * 5 0.5
(21) Perfume appropriate amount * 1: Benton 38 (manufactured by NL Endowry)
* 2: Avil EM-90 (Gold Schmidt)
* 3: KF-6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 4: KF-7312J (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 5: Extract prepared in Example 1

  The prepared cream foundation has a smooth feel without stickiness, has excellent adhesion to the skin, has a beautiful finish, gives emollient feeling to the skin, exhibits skin thickening suppression action, and anti-inflammatory action. After all, it was a cream foundation that can make beautiful skin with elasticity.

  ADVANTAGE OF THE INVENTION According to this invention, the novel interleukin 6 production inhibitor with favorable safety | security and the skin external preparation or cosmetics using the anti-inflammatory effect or skin thickening inhibitory effect using the same can be provided. .

Claims (3)

An interleukin-6 production inhibitor comprising a component derived from peanut sprouts as an active ingredient. A skin external preparation or cosmetic for suppressing skin thickening, comprising the interleukin 6 production inhibitor according to claim 1. An anti-inflammatory skin external preparation or cosmetic comprising the interleukin 6 production inhibitor according to claim 1.