JP2010095475A - Essence extracted from jasmine flower and composition for inhibiting increase of blood lipid level - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a novel utilization field of essence extracted from a jasmine flower obtained from a jasmine flower, particularly a flower of Jasminum sambac. <P>SOLUTION: A composition for inhibiting increase of a blood lipid level comprising as an essential ingredient an extract of a jasmine flower, particularly a flower of Jasminum sambac, is provided. Oral administration of the extract from the jasmine flower, particularly the extract from the flower of Jasminum sambac, in succession to ingestion of lipid, inhibits increase of a blood serum triglyceride level. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an extract of jasmine flowers obtained from jasmine flowers, in particular, pine flowers, and uses thereof.

  Jasmine is a general term for plants of the genus Sossei (Jaminaceae Jasminum), and about 300 species are known in the world. For example, Jasminum beesianum, Jasminum floridum, Jasminum grandiflorum, Jasminum humile, Jasminum humile var. Glabrum, Jasminum voile. Jasminum nudiflorum, Jasminum marianum, Jasminum mesnyi, Borneo, Jasminum multiflorum, Jasminum nervosum var. Elegans, Jasminum nuidum, um ), Jasminum polyanthum, pine (Jasminum sambac), and Okinawa quail (Jasminum sinense).

In some species of jasmine, a plant of the genus Sokei, the flowers have a strong fragrance and are used for perfume and jasmine tea. In particular, mackerel is cultivated on a large scale in China, Southeast Asia and the like for flavoring jasmine tea.
The incense of jasmine tea is known to be picked in the daytime when the buds are still blossoming (buds), and mixed with tea leaves when the flowers begin to open at night. .

  Regarding the usage of the jasmine extract obtained from jasmine, the following usages are disclosed in addition to the perfumes and flavoring agents of jasmine tea as described above.

  For example, Patent Literature 1 discloses a lipolysis promoter containing jasmine extract as an active ingredient, and Patent Literature 2 discloses a slimming food characterized by containing an extract of jasmine flowers. Reference 3 discloses an SCF binding inhibitor containing pine or its extract as an active ingredient, and pine or its extract has an SCF binding inhibitory activity, and causes pigmentation and stains caused by excessive production of melanin.・ It is described that it is effective in preventing or improving buckwheat.

  In addition, Non-Patent Document 1 and the like disclose lipase activity inhibitors containing jasmine tea powder or jasmine tea extract as an active ingredient, but as described above, jasmine tea is made by contacting pine cricket with tea leaves, After that, this mash is removed, and the scent of jasmine is added to the tea leaves. Since the components derived from jasmine are volatile components and are only easily adsorbed by the tea leaves, they contain only a very small amount. However, it is common sense that the inhibitory action of the lipase activity inhibitor is due to the action of tea leaf extract components, particularly catechin.

JP 2000-016916 A JP 2003-310213 A JP 2008-31089 A 14th and 90th Annual Meeting of the Japanese Society of Pharmaceutical Sciences, “The anti-obesity effect of jasmine tea”

  An object of the present invention is to provide a new jasmine flower extract extract obtained from a jasmine flower, particularly a pine flower, and a new use of the same.

The present inventor has found that an increase in serum triglyceride can be suppressed by ingesting an extract of jasmine flowers, in particular, pine flower, orally following a lipid in a test using rats. This is what I thought of.
That is, the present invention proposes a composition for inhibiting blood lipid elevation, which contains an extract of jasmine flowers, particularly pine squirrel (Jasminum sambac), as an active ingredient.

General jasmine tea and its extract cannot be expected to suppress blood lipid elevation, but the extract of jasmine flowers, particularly pine (Jasminum sambac) flowers, is confirmed to have an effect of inhibiting blood lipid elevation. I was able to.
The jasmine flower extract has blood jail-inhibiting effect as well as jasmine fragrance, so it is excellent in fragrance and has no bitterness like tea. An intermediate lipid elevation inhibitor can be provided.

BEST MODE FOR CARRYING OUT THE INVENTION

  Hereinafter, although embodiment of this invention is described, this invention is not limited to the following embodiment.

(Jasmine flower extract)
The jasmine flower extract (hereinafter referred to as “the present jasmine flower extract extract”) according to the present embodiment is an extract obtained by extracting jasmine flowers, that is, jasmine petals with water, lower alcohol, or a mixture thereof. .

As described above, jasmine targeted by the present invention is a general term for plants belonging to the genus Sossei (Jaminaceae Jasminum), and about 300 species are known. For example, Jasminum beesianum, Jasminum floridum, Jasminum grandiflorum, Jasminum humile, Jasminum humile var. Glabrum, Jasminum voile. Jasminum nudiflorum, Jasminum marianum, Jasminum mesnyi, Borneo, Jasminum multiflorum, Jasminum nervosum var. Elegans, Jasminum nuidum, um ), Jasminum polyanthum, Matsurika (Jasminum sambac), Okinawa quail (Jasminum sinense), and the like.
Among these, as a raw material plant of the present jasmine flower extract, pine (Jasminum sambac) is particularly preferable.

  Jasmine flowers include unflowered camellia, unflowered camellia petals, petals immediately after flowering, and petals that are slightly browned after flowering, preferably petals that have just flowered and slightly browned after flowering. .

The solution used for extraction (referred to as “extraction solution”) may be water, lower alcohol, or a mixture thereof.
At this time, examples of the water include city water, hard water, soft water, ion exchange water, ascorbic acid-containing aqueous solution, and pH-adjusted water.
The lower alcohol includes alcohols having less than 8 carbon atoms such as methanol and ethanol, and ethanol is particularly preferable.

Extraction can be performed by leaching jasmine flowers in the extraction solution and stirring as necessary.
Moreover, it is also possible to employ | adopt the method normally performed industrially. For example, an extraction device, a Soxhlet extraction device, or a liquid in a predetermined amount of hot water or the like filled with a raw material in a column and sequentially fed a liquid such as hot water to the column to obtain an extract. Depending on the amount of raw materials to be processed, such as an extraction device called a kneader that is immersed for a certain period of time, an extraction device using a distillation method such as steam distillation, an extraction device using a supercritical extraction method, an extraction device using a pressing method, etc. The preferred extraction device may be selected as appropriate for extraction, and solid-liquid separation may be performed by a normal method. There is no particular limitation on the method, and it can be selected as desired or desired.

The temperature of the extraction solution is not particularly limited, and it is not particularly necessary to adjust the temperature. For example, it may be adjusted to 20 to 100 ° C., particularly 40 to 95 ° C., particularly 60 to 90 ° C.
The pH of the extraction solution is not particularly limited.

There is no particular limitation on the amount of the extraction solution, for example, preferably 5 mL / g to 50 mL / g, more preferably 5 mL / g to 40 mL / g, and more preferably 10 mL / g to jasmine flowers. More preferably, it is 30 mL / g.
There is no particular limitation on the extraction time, for example, preferably 1 minute to 500 minutes, more preferably 5 minutes to 300 minutes, and still more preferably 5 minutes to 200 minutes.

After extraction, the extract is separated from the extraction residue by filtration through a filter net, filter cloth, etc., and further subjected to solid-liquid separation by centrifuging as necessary to obtain a jasmine extract.
In this case, examples of the filtration method include a filtration method using a mesh filter, a paper filter, a stainless steel filter, a flannel cloth, and a strainer, and these can be appropriately employed.

The jasmine extract thus obtained may be brought into contact with a synthetic adsorbent to adsorb and remove organic substances in the extract.
At this time, as a method of contacting with the synthetic adsorbent, the synthetic adsorbent may be added to the jasmine extract and stirred, followed by solid-liquid separation, or the column may be filled with the synthetic adsorbent. The extract may be passed through.
The synthetic adsorbent to be contacted may be a crosslinked polymer having a porous structure having pores of several tens to several hundreds of angstroms and can adsorb various organic substances from the solution. For example, HP series, SP700, SP800, SP70 series, SP207, and methacrylic ester HP2MG manufactured by Mitsubishi Chemical Corporation can be used.

The jasmine extract thus obtained can be subjected to treatments such as freezing, drying, and concentration according to the purpose to obtain a jasmine flower extract.
A known method may be employed as the method for concentrating and drying the mixed solution, and examples of the concentration method include vacuum concentration, and examples of the drying method include freeze drying and spray drying.

  Moreover, you may sterilize as needed. As the sterilization method, a known sterilization method can be employed. For example, autoclave sterilization can be employed.

  The component composition of the jasmine flower extract is still unknown, but it is known that it contains at least almost no catechins. At most, the concentration of catechins is 1% by mass or less.

(Blood lipid elevation inhibiting composition)
The blood lipid elevation inhibitory composition (hereinafter referred to as “main blood lipid elevation inhibitory composition”) according to this embodiment comprises a jasmine flower extract, for example, the present jasmine flower extract extract as an active ingredient.

As shown in the test to be described later, it has been confirmed that when the present jasmine flower extract extract is orally ingested after the lipid is orally ingested, an increase in serum triglyceride can be suppressed.
Triglyceride is important as an energy source for animal activity and is absorbed from the diet or synthesized in the body, but excess triglyceride accumulates as fat around the viscera and subcutaneously and induces obesity. This may also promote type 2 diabetes and hypertension. Moreover, the correlation with hypertriglyceridemia has also been clarified from the relation with arteriosclerosis. In recent years, in view of dietary habits and exercise habits that have attracted particular attention in lifestyle habits, that is, drugs that regulate blood triglyceride concentration are useful in view of overeating and lack of exercise.
Incidentally, hyperlipidemia (a pathological condition in which lipids in the blood increase) is classified into hypercholesterolemia in which only serum cholesterol increases and hypertriglycerideemia in which only triglyceride increases.

The composition for inhibiting blood lipid elevation can be provided as an orally administered drug, a quasi-drug, or a dietary supplement (supplement).
At this time, the dosage form can be prepared, for example, in the form of a liquid, tablet, powder, granule, sugar-coated tablet, capsule, suspension, emulsion, pill and the like.
Regarding formulation (formulation), commonly used excipients, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, solubilizers, It can be produced by a conventional method using a preservative, a flavoring agent, a soothing agent, a stabilizer and the like. Also, for example, lactose, fructose, glucose, starch, gelatin, magnesium carbonate, synthetic magnesium silicate, talc, magnesium stearate, methylcellulose, carboxymethylcellulose or salts thereof, gum arabic, polyethylene glycol, syrup, petrolatum, glycerin, ethanol, propylene Non-toxic additives such as glycol, citric acid, sodium chloride, sodium sulfite, and sodium phosphate can be blended.
In addition, when preparing as a quasi-drug, it can make it easy to ingest, for example by setting it as drinking forms, such as a bottle drink, or forms, such as a tablet, a capsule, and a granule.

In addition, by adding the present blood lipid elevation inhibiting composition to various foods and drinks, it is possible to prepare health foods, health drinks, foods for specific health use, functional foods, etc. that have the effect of inhibiting blood lipid elevation. .
For example, carbon dioxide, excipients (including granulating agents), diluents, or sweeteners, flavors, flours, starches, sugars, oils and other various proteins, carbohydrate raw materials, vitamins, minerals and other food materials Mixed with one or more selected, or currently known foods and beverages such as sports drinks, fruit drinks, milk drinks, tea drinks, vegetable juices, dairy drinks, alcoholic drinks, jelly, jelly drinks, carbonic acid It can be produced by adding to beverages, chewing gum, chocolate, candy, biscuits, snacks, bread, dairy products, fish paste products, livestock meat products, frozen desserts, dried foods, supplements and the like.

  When this blood lipid rise inhibitory composition is added to various foods and drinks, not only the blood lipid rise inhibitory effect can be obtained, but also the scent of jasmine can be obtained.

  With regard to the safety of the present composition for suppressing the increase in lipids in the blood, it can be said that the safety is guaranteed because the active ingredient is a component extracted from jasmine that has been taken orally by mankind for many years.

(Explanation of terms)
In the present specification, when expressed as “X to Y” (X and Y are arbitrary numbers), unless otherwise specified, “preferably greater than X” and “preferably Y” together with the meaning of “X to Y”. It means “smaller”. Further, X and Y at that time are numerical values considering rounding.
Further, when expressed as “X or more” or “Y or less” (X and Y are arbitrary numbers), it means “preferably larger than X” or “preferably smaller than Y” unless otherwise specified. To do. Further, X and Y at that time are numerical values considering rounding.

  Next, although this invention is further demonstrated based on a test example, this invention is not limited to the Example shown below.

(Test 1)
100 g of flower petals of jasmine (Masica) were immersed in 2 L of 60% ethanol (20 ° C.) and extracted with appropriate stirring at room temperature for 3 hours. Thereafter, solid-liquid separation was performed using a mesh filter, and the collected liquid was concentrated under reduced pressure and freeze-dried to obtain 20.1 g of a jasmine flower extract (referred to as “flower EE”).

Next, the blood triglyceride rise inhibitory effect of the jasmine flower extract was evaluated by a lipid tolerance test.
A 7-week-old male SD rat was acclimated for 1 week and then subjected to a lipid tolerance test.
A lipid emulsion prepared by suspending 200 g of soybean oil, 12 g of egg lecithin, and 22.5 g of glycerol in 1000 mL of water by sonication was orally administered at 10 mL / kg of body weight, then 5 mL of water was added to the control group, and the flower extract For the group, jasmine flower extract ("Hana EE") was dissolved in water and orally administered at a concentration of 500 mg / kg body weight / 5 mL.
Blood was collected from the tail vein before administration (0 hour) and 1, 2, 3, 4, and 5 hours after administration, and serum triglyceride was obtained using Lab Assay TM triglyceride (manufactured by Wako Pure Chemical Industries). The results are shown in Table 1. The numerical values in Table 1 are serum triglyceride (TG) concentrations.

As shown in Table 1, the control group showed a maximum value within 2 to 3 hours after lipid loading.
On the other hand, in the flower EE group, the value after 2 to 3 hours showing the maximum value of TG increase showed a low value, and the TG increase inhibitory effect was recognized.
In this test, an extract of ethanol is tested, but when extracted with other lower alcohols, it is expected that similar components can be extracted, and it can be assumed that similar effects can be enjoyed.

(Test 2)
100 g of flower petals of jasmine (Matsurika) were immersed in 2 L of ion-exchanged water and extracted with appropriate stirring at 85 ° C. for 15 minutes. Solid-liquid separation was performed using a mesh filter and centrifugation, and the collected liquid was concentrated under reduced pressure and spray-dried to obtain 18.6 g of jasmine flower extract (referred to as “flower WE”).
In addition, the extract obtained by extracting jasmine flowers in the same manner as described above was passed through a column packed with 250 mL of a synthetic adsorbent (styrene divinyl-based synthetic adsorption resin, SP207 manufactured by Mitsubishi Chemical Corporation), After washing, 60% ethanol was passed through to collect the eluted components, which were concentrated under reduced pressure and spray-dried to obtain 5.2 g of jasmine flower extract (referred to as “flower WE / SP”).
Note that SP207 manufactured by Mitsubishi Chemical is an aromatic synthetic adsorbent in which bromine is introduced into an aromatic ring and can adsorb particularly highly hydrophilic organic substances because of the strong hydrophobicity of the pore surfaces.

  Lipid tolerance test (8 animals per group) was performed in the same manner as in Test 1, and serum triglycerides before and after administration were measured. The results are shown in Table 2. The numerical values in Table 2 are serum triglyceride (TG) concentrations.

From the results in Table 2, the control group showed a maximum value within 2 to 3 hours after lipid loading.
In contrast, the flower WE group showed a particularly low value at 2 to 4 hours after administration compared to the control group, and the flower WE was confirmed to have an inhibitory effect on the increase in serum TG.
Similarly, in the flower WE / SP group, it was confirmed that the value was particularly low in 2-3 hours after administration, and the synthetic adsorbent SP207 adsorbing component in the flower WE extract was confirmed to have an effect of suppressing the increase in serum TG. It was done.

(Test 3)
The extracted components of jasmine tea leaves (green tea leaves with scented jasmine flowers incense) that are perfumed in green tea leaves with jasmine (pine tree) flowers were compared with the extracted components of jasmine (matsuri) flowers.

Weighed 6.5 g of each of the three types of jasmine teas A, B, and C (jasmine flower incense green tea leaves made by fragrant green flowers), and put each in 1 L of distilled water and stirred. Extracted at 82 ° C. for 3.5 minutes, separated into solid and liquid, cooled to room temperature, added ion-exchanged water to the resulting extract, adjusted to Brix 0.20%, and filtered through a filter (0.2 μm). Thus, a sample for analysis was prepared (“Jasmine tea A, B, C extract” in Table 4).
In addition, 6.5 g of pine flower was weighed, poured into 1 L of distilled water, extracted with stirring at 82 ° C. for 3.5 minutes, then separated into solid and liquid, cooled to room temperature, and the resulting extract. Ion-exchanged water was added to adjust the Brix to 0.20%, and filtered through a filter (0.2 μm) to obtain a sample for analysis (“Jasmine flower extract” in Table 4).
Also, ion-exchanged water was added to a commercially available PET bottled jasmine tea beverage to adjust to Brix 0.20%, and then filtered through a filter (0.2 μm) to obtain a sample for analysis ("Jasmine tea beverage" in Table 4). ).
Furthermore, the jasmine flower extract (referred to as “flower WE”) obtained in Test 2 above was dissolved in ion-exchanged water, adjusted to Brix 0.20%, filtered through a filter (0.2 μm), and an analytical sample. ("Jasmine flower extract" flower WE "" in Table 4).

  Catechin and caffeine analysis were performed as component analysis. The analysis was performed by HPLC analysis using UPLC manufactured by Waters. AQCUITY UPLC BEH Shield RP18 was used as the column, and the column method was performed at a column temperature of 40 ° C. by the gradient method (see Table 3). The moving bed A solution was 0.1% phosphoric acid aqueous solution, the B solution was 0.1% phosphoric acid-containing acetonitrile, the sample injection was 5.0 μL, and the detection was performed with PDA. As catechins, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCg), gallocatechin gallate (GCg), epicatechin gallate (ECg), Eight species of catechin gallate (Cg) and caffeine (Caf) were quantified.

From the analysis results, it was found that catechins were not detected in the jasmine flower extract and the jasmine flower extract “Hana WE”, and the catechins were not contained in the extract components of the jasmine flower.
In addition, the specific ingredients as jasmine flower extract components are not included in the extract of jasmine tea and jasmine tea beverage, and clearly the composition of jasmine flower extract and jasmine tea extract are completely different from each other. I understood.

Claims (6)

  A composition for suppressing blood lipid elevation, comprising an extract of jasmine flowers as an active ingredient.   The composition for suppressing blood lipid elevation according to claim 1, wherein the extract of jasmine flowers is obtained by extracting the petals of jasmine with water, a lower alcohol or a mixture thereof.   The composition for suppressing blood lipid elevation according to claim 1, wherein the extract of jasmine flowers is obtained by extracting the petals of flowered jasmine with water, a lower alcohol, or a mixture thereof.   The composition for inhibiting blood lipid elevation according to claim 1 or 2, wherein the total amount of catechin is 1% by mass or less.   An extract of jasmine flower obtained by extracting the petals of jasmine with water, lower alcohol or a mixture thereof.   A jasmine flower extract obtained by extracting flowered jasmine petals with water, lower alcohol or a mixture thereof.