JP2010038666A - Ammonia inspecting instrument and sensitivity adjusting method of ammonia inspecting instrument - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an ammonia inspecting instrument for precisely detecting and measuring ammonia in saliva in a short time by simple operation, and an ammonia inspecting method. <P>SOLUTION: The ammonia inspecting instrument has an indicator layer 2, which causes color reaction by ammonia, on a support 1. The indicator layer is covered with a film 4 formed from a covering agent aqueous solution containing a polyvinylpyrrolidone and a surfactant and adjusted to a pH of 2.0-5.0 (25°C), so that the dry mass of the film is 25-50 mass% of the indicator layer and detects or measures ammonia in saliva. As a result, the saliva being a sample is used as a specimen as it is without performing dilution operation to precisely detect or measure ammonia in the saliva in a short time by simple operation and an aspect from safety or an aspect from environment is also good. Further, a bad breath or the number of bacteria in the saliva can be estimated from the detected or measured result of ammonia in the saliva and sanitariness in the oral cavity can be judged using the detected or measured result as an index. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an ammonia test device capable of detecting ammonia contained in saliva or measuring the concentration thereof quickly, simply and accurately without performing a dilution operation of saliva (specimen), and a sensitivity adjustment method thereof. About.

  Factors affecting the cleanliness of the oral cavity include the presence of plaque (plaque) and tartar, the amount of tongue coating, turbidity of saliva, the number of bacteria in the oral cavity, and the like. As a method that can easily check these factors, for example, for plaque, tartar, and tongue coating, pigments such as erythrosine and phloxine, the fluorescent dye fluorescein sodium, and new and old plaques are color-coded. There is a method of discriminating the presence or absence of staining using a dichroic plaque stain agent Totone (manufactured by Morita Co., Ltd.).

  However, the methods using these dyeings have low selectivity of the colored portion depending on the dye used, and portions that do not need to be stained, such as lips and mucosal cells, are also stained, and it takes a long time to fade. There was also a problem with aesthetics.

  As an inspection method by measuring the turbidity of saliva, a saliva property inspection kit and an oral disease determination method (see Patent Document 1: Japanese Patent Laid-Open No. 2004-108976) are proposed, and the turbidity of saliva is between bad breath and It is shown that there is a close correlation. However, this method visually determines the amount of insoluble substances in saliva. However, it is difficult to evenly disperse the components of saliva, resulting in variations in measured values and inferior accuracy.

  As a method of examining the number of bacteria in the oral cavity, generally, a method is used in which a sample is diluted and dispersed to a fixed multiple and the number of colonies after culture is counted, but this method is influenced by individual differences in the diluted multiple. In addition, there is a problem that the operation is troublesome, for example, it takes a long time to prepare a medium and culture time.

  Measurement of the amount of bacteria in the oral cavity using a monoclonal antibody against a specific bacterium (Patent Document 2: JP-A-60-73463, Patent Document 3: JP-A-62-211558), using a DNA or RNA probe A method for measuring the number of bacteria and cells (see Japanese Patent Application Laid-Open No. Sho 61-257200) has also been proposed. However, since these methods use special and expensive reagents, they are economically problematic for use by a wide range of people in group medical examinations and health education.

  On the other hand, it is known that ammonia has a high correlation with factors such as bad breath and the number of bacteria in saliva (see Non-Patent Document 1: J. Dent. Res. Abstracts # 1830, 2006). Therefore, the applicant considered that it is possible to estimate the cleanliness in the oral cavity by using ammonia in saliva as an index, and studied means for measuring ammonia in saliva. However, while the detection means for detecting or measuring ammonia, such as the known ammonia test paper, is suitable for water quality testing and measurement of ammonia concentration in blood, its sensitivity is high, so it is easy to accurately measure ammonia in saliva. It was unsuitable to detect or measure well.

  Therefore, it is desired to develop a test means that can detect or measure ammonia in saliva easily and accurately in a short time and that is useful for estimating the cleanliness of the oral cavity using ammonia in saliva as an index.

JP 2004-108976 A JP 60-73463 A JP-A-62-211558 JP-A-61-257200 Japanese Patent Laid-Open No. 58-193459 J. et al. Dent. Res. Abstracts # 1830, 2006

  The present invention has been made in view of the above circumstances, and provides an ammonia test tool capable of detecting ammonia in saliva or measuring the concentration thereof in a short time and with a simple operation, and a sensitivity adjustment method thereof. With the goal.

  As a result of intensive studies to achieve the above object, the inventors of the present invention provide an ammonia test tool having an indicator layer that is colored by ammonia on a support, and the indicator layer has a surface activity with polyvinylpyrrolidone. And a coating formed with a coating aqueous solution adjusted to pH 2.0 to 5.0 (25 ° C.), and the dry mass of the coating is 25 to 50% by mass of the indicator layer Thus, the sensitivity of the indicator layer is adjusted, and the saliva of the sample is used as it is as a specimen without diluting operation, and ammonia in saliva is detected or its concentration is accurately detected in a short time and with simple operation. It has been found that an ammonia tester that can be measured is obtained.

That is, the present inventors focused on ammonia showing a high correlation with factors such as bad breath and the number of bacteria in saliva as an index for judging the cleanliness of the oral cavity, and examined an inspection means for detecting or measuring ammonia in saliva. did.
An analyzer for measuring the concentration of ammonia or urea in a liquid sample is proposed in Patent Document 5: Japanese Patent Laid-Open No. 58-193259, etc., and an ammonia test for measuring water quality and blood ammonia concentration. Various papers are commercially available.
For example, Amicheck (trade name, manufactured by Kyoto Daiichi Kagaku Co., Ltd.) measures ammonia in blood, serum, or plasma for the purpose of understanding pathological conditions such as abnormal amino acid metabolism, abnormal liver function, and abnormal renal function. It is. From the package insert, it is considered that the ammonia measurement concentration range is 0.1 to 4 μg / mL, and the blood ammonia reference value is 0.18 to 0.70 μg / mL. Become. Aqua Check A (trade name, manufactured by Bayer Medical Co., Ltd.), a simple water quality test paper, can measure 0.1 to 10 μg / mL of ammonia. Test stick ammonia (trade name, manufactured by Nisso Co., Ltd.) can measure 0.1 to 5 μg / mL of ammonia, and is in a range of measurement concentration preferable for water quality inspection in aquarium of tropical fish and ornamental fish.

However, since the ammonia concentration in the adult saliva is in the range of about 10 to 1,000 μg / mL (see J. Dent. Res. Abstracts # 1830, 2006), the commercially available test paper is too sensitive to the measurement in the saliva. It was unsuitable for accurately detecting or measuring ammonia. In addition, the merquaqua ammonium test (trade name, manufactured by MERCK) has a measuring range of 0.1 to 400 μg / mL and can measure ammonia up to a higher concentration, but uses toxic mercury (II) iodide. As a result, there were problems in terms of safety and environment.
Table 1 shows the ammonia measurement range of these commercially available ammonia test papers and the ammonia concentration in saliva.

  If saliva collected from the mouth is diluted to an appropriate concentration in advance and this diluted saliva is used as a sample, it is possible to detect or measure ammonia in the saliva using the above-mentioned commercial product, but the operation of diluting saliva Is cumbersome and cumbersome, and the process becomes complicated and takes a long time. These factors may adversely affect the measurement accuracy. It is desirable that examinations performed in group examinations, health education projects, etc. are simple and inexpensive and can obtain examination results in a short time.

  According to the ammonia test tool of the present invention, the above-mentioned problems can be solved, and by coating with a film obtained by immersing the determination part (indicator layer) of the test tool in a specific coating solution and drying it, ammonia and The development time until the color development of the indicator layer due to the reaction with the indicator is adjusted and the judgment sensitivity is lowered, so that the saliva is used as it is as a specimen without diluting it, and ammonia is detected or its Concentration can be measured with high accuracy and simple operation.

Accordingly, the present invention provides the following ammonia test tool and sensitivity adjustment method thereof.
(I) An ammonia test tool having an indicator layer that reacts with ammonia on a support, wherein the indicator layer contains polyvinylpyrrolidone and a surfactant and has a pH of 2.0 to 5.0 (25 ° C. ) And a coating formed with a coating aqueous solution prepared in the above, and the dry mass of the coating is 25 to 50% by mass of the indicator layer, and is for detecting or measuring ammonia in saliva. An ammonia tester characterized by that.
(Ii) The above-described ammonia test tool for detecting or measuring ammonia in saliva to estimate the cleanliness of the oral cavity.
(Iii) The indicator layer of the ammonia tester having an indicator layer that reacts with color by ammonia on the support is adjusted to pH 2.0 to 5.0 (25 ° C.) containing polyvinylpyrrolidone and a surfactant. In order to detect or measure ammonia in saliva, which is characterized by adjusting the sensitivity by immersing in a coating aqueous solution, drying and coating with a film whose dry mass is 25 to 50% by mass of the indicator layer Sensitivity adjustment method for ammonia testing tools.

  According to the ammonia test tool of the present invention and its sensitivity adjustment method, the saliva of the sample is used as it is as a sample without diluting, and ammonia in saliva is detected accurately in a short time and with a simple operation. Or it can be measured, and the safety and environmental aspects are also good. Furthermore, according to the present invention, the bad breath, the number of bacteria in the saliva, and the like can be estimated from the detection or measurement result of ammonia in the saliva, and the oral cleanliness can be determined using this as an index.

  Hereinafter, the present invention will be described in more detail. The ammonia test tool of the present invention is an ammonia test tool having an indicator layer that is colored and reacted with ammonia on a support, and the indicator layer is a specific coating agent. It is coated with a film formed with an aqueous solution.

  Here, the aqueous coating agent solution used in the present invention is an aqueous solution containing polyvinylpyrrolidone and a surfactant, and is adjusted to pH 2.0 to 5.0 (25 ° C.).

Polyvinylpyrrolidone is a polymer substance as a film-forming component, and preferably has an average molecular weight of 10,000 to 360,000, particularly 10,000 to 160,000. If the average molecular weight is less than 10,000, the viscosity stability is low, If it exceeds 360,000, the viscosity stability is high, but quality problems such as precipitation may occur.
The average molecular weight was measured by applying the relative viscosity value (25 ° C.) measured by a capillary viscometer to the following Fikentscher formula using the viscosity characteristic value (K) correlated with the molecular weight. Value.

  A commercially available product can be used for polyvinylpyrrolidone, for example, Rubiscol K15 (average molecular weight 10,000), K30 (average molecular weight 40,000), K60 (average molecular weight 160,000), K90 (average molecular weight 360,000) (all of which are BASF Japan Ltd.) These may be used singly or in combination of two or more.

  The blending amount of polyvinyl pyrrolidone is preferably 10 to 40%, particularly 25 to 40% (mass%, the same shall apply hereinafter), particularly 26 to 38%, based on the total aqueous coating agent solution. If it is less than 10%, it is difficult to see the difference in the concentration of saliva in ammonia, and if it exceeds 40%, the reaction between the indicator layer of the test paper and the ammonia takes 3 minutes or more, and quick determination may not be possible.

A surfactant is further blended in the coating agent. By adding a surfactant, it is easy to coat the indicator layer of the ammonia test paper, which is advantageous.
As the surfactant, an anionic surfactant, a nonionic surfactant, a cationic surfactant, or the like can be used. For example, alkyl sulfates such as lauryl sulfate, lauroyl sarcosinate, alkylaryl polyethylene glycol, amino acid sugar ester compounds, fatty acid sugar esters, cetylpyridinium chloride and the like can be mentioned.

  Of these, anionic surfactants such as alkyl sulfates and ralloyl sarcosinates, and nonionic surfactants such as alkylaryl polyethylene glycols are preferred for maintaining the stability of the coating agent and surfactant. .

  The compounding amount of the surfactant is preferably 0.3 to 1.2%, particularly preferably 0.5 to 1.0% of the whole coating solution. If it is less than 0.3%, it is difficult to form a film by the coating agent, and if it exceeds 1.2%, a problem may occur in the stability of the coating agent.

  If necessary, a known component such as Sephadex G-25, G-50, or G-100 (manufactured by GE Healthcare Biosciences), which is a gel filtration agent, may be added to the coating agent as necessary. Good.

In the present invention, the pH of the aqueous coating solution solution is set in the range of 2.0 to 5.0, preferably 2.0 to 4.6. By adjusting the pH to this range, the color change of the reagent contained in the indicator layer can be effectively suppressed. If the pH exceeds 5.0, discoloration occurs due to a decrease in the stability of the indicator layer. If the pH is less than 2.0, the color development degree of the indicator layer may be reduced, and ammonia determination may be difficult.
In addition, a pH adjuster can be used for pH adjustment as needed. As the pH adjuster, an acid such as acetic acid, phosphoric acid, hydrochloric acid, citric acid, lactic acid, gluconic acid or a salt thereof can be used.

  In the ammonia test tool of the present invention, the indicator layer of the ammonia test tool having an indicator layer that reacts with color by ammonia is coated on a support with a film formed of the aqueous coating solution, and the dry mass of the film is It is 25 to 50% of the dry mass of the indicator layer.

  Here, as an ammonia tester having an indicator layer that reacts with color by ammonia on the support, if it has a layer containing an indicator on part or the entire surface of the support such as paper, plastic, etc. There are no particular restrictions on the material, components, structure, etc., and it may be an ammonia tester having an indicator layer on a support and a carrier containing an alkaline buffer that gasifies ammonia in saliva, and is a known ammonia test. Ammonia testing instruments such as paper can be used that have no safety or environmental problems.

  Specifically, a commercially available product can be used as the above-described ammonia test tool, such as Amicheck (trade name, manufactured by Kyoto Daiichi Kagaku Co., Ltd.), Aqua Check A (trade name, manufactured by Bayer Medical Co., Ltd.), Test Stick Ammonia. (Trade name, manufactured by Nisso Corporation).

  In addition, the shape of the support of the ammonia test tool is preferably a stick-like shape with a width of 3 to 7 mm, a length of 50 to 100 mm, and a thickness of 0.2 to 0.4 mm from the viewpoint of usability. Those having a length of 82 mm and a thickness of 0.25 mm are preferred.

As the indicator contained in the indicator layer, for example, bromthymol blue, thymol blue, bromophenollet, phenol red, bromocresol green and the like can be used, and an indicator used in a commercially available ammonia test device can be used.
Are preferable shape of the total area 9～49Mm ² of the indicator layer, it is suitable in particular those of 25 mm ^2.

  In the present invention, the method of coating the indicator layer on the support with the coating formed with the aqueous coating solution is not particularly limited. After the indicator layer is immersed in the aqueous coating solution for 60 to 600 seconds and pulled up, A method of air-drying for 24 to 72 hours at room temperature, or after dripping an aqueous coating solution onto the indicator layer and confirming that the entire surface of the indicator layer has been coated, leave it at room temperature for 5 to 60 minutes, Thereafter, a method of air-drying at room temperature for 24-72 hours can be employed.

The coating film formed by the aqueous coating solution has a dry mass in the range of 25 to 50%, preferably 28 to 47%, of the indicator layer, thereby diluting ammonia in saliva in a short time of about 1 minute. Judgment without operation If the dry mass is less than 25%, the color tone of the blank (indicator layer) changes from yellow to light yellowish green, making visual judgment difficult, and if it exceeds 50%, the judgment takes a long time and judgment takes a short time. Becomes difficult.
Further, the dry mass of the coating film is preferably 1 to 3 mg, particularly preferably 1.3 to 2.6 mg.
In addition, the said dry mass is a mass after drying an indicator layer or a coating film for 2 days at 40 degreeC.
A film having a dry mass within the above range can be obtained by forming a film using an aqueous coating solution containing 10 to 38% of a coating agent (polyvinylpyrrolidone).

  Specific examples of the ammonia testing tool of the present invention are shown in FIGS. 1 and 2 show an ammonia test paper (commercially available ammonia test paper, Aqua Check A (trade name, manufactured by Bayer Medical Co., Ltd.) having an indicator layer 2 and a carrier (alkali layer) 3 on a stick-like support 1. 1) is coated with a coating 4 formed of the aqueous coating solution according to the present invention, Fig. 1 is a schematic sectional view, Fig. 2 is an exploded perspective view, and Figs. As shown in the exploded perspective view of FIG. 3, the ammonia test paper shown in FIG. 3 may be coated with a saliva intrusion prevention cover 5 to limit the saliva infiltration site.

  The ammonia test device of the present invention drops the sample saliva onto the indicator layer (determination site) coated with the coating film, and determines the color tone of the indicator layer after a certain period of time, whereby the presence of ammonia in the saliva The presence or absence of ammonia or the concentration of ammonia can be quantified.

  The saliva used for the specimen may be human saliva or animal saliva, resting saliva, stimulated saliva (saliva collected within a certain period of time after chewing gum or wax), discharged saliva (a certain amount) Any of the above-mentioned distilled water contained in the oral cavity and discharged after a predetermined time can be used.

  Although the test conditions using saliva are not particularly limited, the ammonia test tool of the present invention and 2 mL of saliva are stirred for 30 seconds and then left for 30 to 120 seconds, and the commercially available ammonia test tool used (for example, Aqua Check A) is used. The determination can be made based on the color sample. Determination can be performed in 30 seconds to 1 minute after contact reaction with saliva.

  EXAMPLES Hereinafter, although an experimental example, an Example, and a comparative example are given and this invention is demonstrated in detail, this invention is not restrict | limited to these Examples. In the following examples, “%” means “% by mass” unless otherwise specified.

[Experimental example]
As components used for the coating agent, the solubility of the polymer compounds carboxymethyl cellulose and polyvinyl pyrrolidone shown in Table 2 and the pH of the solution were examined by the following methods. The results are shown in Table 2.

Method for evaluating solubility;
The coating agent is an aqueous solution of a polymer compound (polyvinyl pyrrolidone or carboxymethyl cellulose sodium), and a surfactant and a pH adjuster can be mixed during preparation. After stirring with a stirrer for 12 hours, the solubility was visually determined and evaluated according to the following criteria.
○: Completely dissolved ×; Method for measuring pH of insoluble matter remaining;
For pH, a pH electrode (pH meter D-51, manufactured by HORIBA) was placed in the aqueous coating solution, and the pH value at room temperature was measured.

  From the results in Table 2, it is necessary to form an appropriate film in order to determine ammonia in saliva. As a result of evaluating the concentration of the coating agent adjusted to 20%, sodium carboxymethylcellulose is dissolved compared to polyvinylpyrrolidone. It was found that polyvinyl pyrrolidone was suitable as a polymer material used for the coating material of the ammonia test device because of its poor properties.

Examples and comparative examples
A commercially available ammonia test paper, Aquacheck A (trade name, manufactured by Bayer Medical Co., Ltd.) is used as an indicator layer (area: 5.2 mm × 5.2 mm = 27 mm ² , weight: 5.2 mg), and coating agent As ingredients, polyvinylpyrrolidone (BASF Japan Co., Ltd., Rubiscol K30, abbreviated as PVP in the table), sodium lauryl sulfate (Toho Chemical Industries, Ltd., abbreviated as SDS in the table), pH adjuster Using a 10% aqueous acetic acid solution or a 10% aqueous phosphoric acid solution, a coating aqueous solution having the composition shown in Table 3 was prepared, and an ammonia test paper treated with the coating by the following method was prepared and evaluated. The results are shown in Tables 4 and 5.

Preparation of coating-treated ammonia test paper;
The components shown in Table 3 were dissolved in water, and the aqueous solution was prepared as it was or by adjusting the pH with a 10% acetic acid aqueous solution or a 10% phosphoric acid aqueous solution.
The indicator layer portion of Aquacheck A was immersed in the resulting aqueous coating solution at room temperature for 60 seconds, and then dried at room temperature for 2 days to prepare a coating-treated inspection paper.
Table 3 summarizes the coating composition, pH, total dry mass, coating mass, and coverage of the prepared test papers (Examples 1-4, Comparative Examples 1-5). In addition, the measuring method of pH, the total dry mass and coating material mass of a determination part, and the calculation method of a coverage are as follows.

pH measurement method;
For pH, a pH electrode (pH meter D-51, manufactured by HORIBA) was placed in the aqueous coating solution, and the pH value at room temperature was measured.
Total dry mass (B);
Dry mass coating material mass (A) of the entire test paper judgment site (indicator layer on which the coating film is formed);
The dry mass coverage of the coating film;
From the above total dry mass (B) and coating mass (A), the coverage of the test paper (ratio (mass ratio) of the dry mass of the coating to the indicator layer) was determined.
Coverage (%) = (A / (BA)) × 100

Examination of reaction time in ammonia detection with ammonia test paper using saliva specimens;
Resting saliva collected from three adult subjects was used as a specimen.
Next, using control ammonia test paper and ammonia test paper treated with a coating agent (Example 3), 2.0 mL of sample saliva (resting or stimulating saliva) was dropped onto the ammonia test paper. The change in the color of the ammonia test paper 1 minute and 3 minutes after the dropping was visually judged. The visual judgment was performed according to the following visual evaluation criteria in accordance with the criteria for Aqua Check A. The results are shown in Table 4.
In the present invention, ammonium ions of ammonium chloride are alkaline, pass through the coating layer treated with the gasification by gasification, and develop color in the indicator layer. At that time, it is considered that the pH of the coating layer also acts on gasification. In the indicator layer, the pH indicator contained in the aqua check A reacts with ammonia gas, and the color change after 1 minute is visually judged, whereby the ammonia in the saliva can be measured without diluting operation.

Visual evaluation criteria (ammonia concentration value in each criterion, μg / mL);
The determination of the ammonia concentration was visually determined in the following 6 stages according to the standard color of Aqua Check A, the color change after the ammonia test paper and ammonia standard solution or saliva were reacted for 1 minute.
−: Yellow (0 μg / mL) ±: Light yellowish green (25 μg / mL)
+: Yellowish green (50 μg / mL) ++: Light green (100 μg / mL)
+++: Green (400 μg / mL) +++++: Dark green (900 μg / mL)

  From the results in Table 4, the test paper treated with the coating agent showed no color change in the difference between the ammonia concentration of 400 μg / mL and 900 μg / mL in the determination after 3 minutes (all were +++++), and was visually However, it was found that when 1 minute later was set as the determination time, a color change was recognized from 100 μg / mL to 900 μg / mL, and the ammonia concentration could be visually determined accurately in a short time.

Detection of ammonia in saliva samples using ammonia test paper;
For the ammonia test papers (Examples 1 to 4 and Comparative Examples 1 to 5) having the compositions shown in Table 3, first, the visual judgment of the judgment site (indicator layer) of the ammonia test paper before judgment evaluation is performed based on the same criteria as above. went. Next, ammonia was detected by the same method using the same sample as described above, with a determination time of 1 minute, and evaluated in the same manner. Moreover, it evaluated similarly using water as a test substance, and showed the determination result as a blank value. The results are shown in Table 5.

  From the results in Table 5, the control test paper (without the coating treatment) reacted instantaneously with ammonia in saliva, and after 1 minute the ammonia concentration exceeded 100 μg / mL, and the visual evaluation of all concentrations was +++++ ( Dark green) and could not be identified. In Comparative Examples 1 to 5, a color change was observed due to the difference in ammonia concentration due to the delay effect due to the coating treatment, but no difference was observed between the ammonia concentrations of 400 μg / mL and 900 μg / mL. could not. In the test papers of Comparative Examples 1, 2, and 4, the test paper is already colored before the test, and an increase is also observed in the blank value when water is used as a specimen, which may be inferior in accuracy of ammonia concentration determination. all right.

  On the other hand, in the products of the present invention (Examples), it is possible to detect ammonia in saliva and determine its concentration quantitatively in a short time of 1 minute after contact with a saliva sample, and an increase in the blank value is also observed. There wasn't. Therefore, it is confirmed that the ammonia test paper coated with the aqueous coating agent solution according to the present invention can be used as a specimen without diluting the saliva, and can detect ammonia in the saliva easily or in a short time by measuring or determining its concentration. did it.

  Next, another embodiment of the ammonia test tool of the present invention will be shown. As with the above-described example, all the inspection tools were able to quickly and easily measure the presence or concentration of ammonia in saliva.

Example 5
Coating aqueous solution composition Polyvinylpyrrolidone (Rubicol K30) 26.0 g
Sodium lauryl sulfate 0.7g
pH adjuster (10% phosphoric acid) 0.3mL
Appropriate amount of water
Total 100g
(PH 2.0)
Indicator layer of commercially available ammonia test paper (Bayer Medical Ltd.) (area 27 mm ^2, weight 5.2 mg), immersed for one minute in the coating, and dried at room temperature for 2 days. The total dry mass (B) at this time was 6.70 mg, the coating agent mass (A) was 1.50 mg, and the coating rate when the indicator layer was coated and dried at room temperature for 2 days was 28.8%. there were.

Example 6
Coating agent aqueous solution composition Polyvinylpyrrolidone (rubiscol K30) 31.0 g
Sodium lauryl sulfate 0.7g
pH adjuster (10% acetic acid) 0.3mL
Appropriate amount of water
Total 100g
(PH 3.5)
An indicator layer (area 27 mm ² , weight 5.2 mg) of a commercially available ammonia test paper (Bayer Medical Co., Ltd.) was immersed in the coating agent for 1 minute and dried at room temperature for 2 days. The total dry mass (B) at this time was 7.10 mg, the coating agent mass (A) was 1.90 mg, and the coating rate when the indicator layer was coated and dried at room temperature for 2 days was 36.5%. there were.

Example 7
Coating agent aqueous solution composition Polyvinylpyrrolidone (rubiscol K30) 32.0 g
Sodium lauryl sulfate 1.0g
pH adjuster (10% acetic acid) 0.4mL
Appropriate amount of water
Total 100g
(PH 2.8)
An indicator layer (area 27 mm ² , weight 5.2 mg) of a commercially available ammonia test paper (Bayer Medical Co., Ltd.) was immersed in the coating agent for 1 minute and dried at room temperature for 2 days. The total dry mass (B) at this time was 7.10 mg, the coating agent mass (A) was 1.90 mg, and the coating rate when the indicator layer was coated and dried at room temperature for 2 days was 36.5%. there were.

Example 8
Coating agent aqueous solution composition Polyvinylpyrrolidone (Rubicol K30) 38.0 g
Sodium lauryl sulfate 0.7g
pH adjuster (10% acetic acid) 0.1mL
Appropriate amount of water
Total 100g
(PH 4.6)
Indicator layer of commercially available ammonia test paper (Bayer Medical Ltd.) (area 27 mm ^2, weight 5.2 mg), immersed for one minute in the coating, and dried at room temperature for 2 days. The total dry mass (B) at this time was 7.60 mg, the coating agent mass (A) was 2.40 mg, and the coating rate when the indicator layer was coated and dried at room temperature for 2 days was 46.2%. there were.

Example 9
Coating agent aqueous solution composition Polyvinylpyrrolidone (rubiscol K30) 18.0 g
Polyvinylpyrrolidone (Rubicol K60) 12.0g
Sodium lauryl sulfate 1.0g
pH adjuster (10% phosphoric acid) 0.2mL
Appropriate amount of water
Total 100g
(PH 4.1)
An indicator layer (area 27 mm ² , weight 5.2 mg) of a commercially available ammonia test paper (Bayer Medical Co., Ltd.) was immersed in the coating agent for 1 minute and dried at room temperature for 2 days. The total dry mass (B) at this time was 7.11 mg and the coating agent mass (A) was 1.91 mg. The coating rate when the indicator layer was coated and dried for 2 days at room temperature was 36.7%. there were.

Example 10
Coating aqueous solution composition Polyvinylpyrrolidone (rubiscol K90) 28.0 g
0.8g sodium lauryl sulfate
pH adjuster (10% acetic acid) 0.3mL
Appropriate amount of water
Total 100g
(PH 3.5)
An indicator layer (area 27 mm ² , weight 5.2 mg) of a commercially available ammonia test paper (Bayer Medical Co., Ltd.) was immersed in the coating agent for 1 minute and dried at room temperature for 2 days. The total dry mass (B) at this time was 6.80 mg, and the coating agent mass (A) was 1.60 mg. The coating rate when the indicator layer was coated and dried at room temperature for 2 days was 30.7%. there were.

Example 11
Coating agent aqueous solution composition Polyvinylpyrrolidone (rubiscol K60) 28.0 g
0.8g sodium lauryl sulfate
Sephadex G-25 2.0g
pH adjuster (10% acetic acid) 0.3mL
Appropriate amount of water
Total 100g
(PH 3.5)
An indicator layer (area 27 mm ² , weight 5.2 mg) of a commercially available ammonia test paper (Bayer Medical Co., Ltd.) was immersed in the coating agent for 1 minute and dried at room temperature for 2 days. The total dry mass (B) at this time is 7.51 mg, the coating agent mass (A) is 2.31 mg, and the coating rate is 44.5% when the indicator layer is coated and dried at room temperature for 2 days. there were.

It is sectional drawing which shows one Example of the ammonia test | inspection tool of this invention. It is a disassembled perspective view which shows one Example of the ammonia test | inspection tool of this invention. It is a disassembled perspective view which shows another Example of the ammonia test | inspection tool of this invention.

Explanation of symbols

1 Support 2 Indicator Layer 3 Carrier (Alkali Layer)
4 Film (coating material)
5 Saliva infiltration prevention cover

Claims (3)

  An ammonia tester having an indicator layer that reacts with ammonia on a support, wherein the indicator layer contains polyvinylpyrrolidone and a surfactant and is adjusted to pH 2.0 to 5.0 (25 ° C.). It is coated with a film formed with the aqueous coating agent solution, the dry mass of the film is 25 to 50% by mass of the indicator layer, and is for detecting or measuring ammonia in saliva Ammonia testing tool.   The ammonia test tool according to claim 1, which is for detecting or measuring ammonia in saliva and estimating cleanliness in the oral cavity.   The said test substance layer of the ammonia test device which has the indicator layer which carries out a color reaction with ammonia on a support body contains polyvinylpyrrolidone and a surfactant and is adjusted to pH 2.0 to 5.0 (25 ° C.) Ammonia test for detecting or measuring ammonia in saliva, characterized by adjusting the sensitivity by dipping in an aqueous solution of the agent and then drying and coating with a film having a dry mass of 25 to 50% by mass of the indicator layer How to adjust the sensitivity of the tool.

Images