JP2010030951A - Toxic substance removing material and toxic substance removing method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a toxic substance removing material efficiently trapping a toxic substance derived from microorganisms such as bacteria and viruses and promptly inactivating the same to minimize the effect to a human body, as well as having good preservability of antibodies, and a long life time. <P>SOLUTION: The toxic substance removing material includes a carrier having an antibody and an antioxidative substance carried thereon. The antioxidative substance preferably contains at least one antioxidative vitamin. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a harmful substance removing material capable of selectively inactivating bacteria or viruses, and a harmful substance removing method using the same.

  In recent years, infectious diseases caused by bacteria, molds, viruses, and the like have become a social problem. For example, there are concerns about mass infection in hospitals and places where a large number of unspecified people gather such as public facilities. In particular, infection in hospitals may cause the occurrence of MRSA (methicillin-resistant Staphylococcus aureus) and the like due to the abuse of antibiotics.

  In this regard, in recent buildings, ducts are provided in all rooms, and air is circulated through these ducts to adjust the room temperature etc. of the entire building, so that the inside of the facility floats through this air conditioner. Bacteria, molds, viruses, and the like often diffuse throughout the facility, and it has been considered that it is particularly effective to block infection routes using such air as a medium. In other words, bacteria, mold, viruses, or fine suspended matters in the air (dust, etc.) are adsorbed on fine filters as air media such as air conditioners and air purifiers, and titanium oxide or strong acidity. The sterilization zone is provided to inactivate and remove bacteria, molds, viruses, and the like that pass through the sterilization zone.

  However, in the removal by adsorption, if harmful substances are bacteria, viruses, etc., the bacteria once collected by the filter may be detached and activated again, affecting the human body. In addition, in the method of inactivation by passing through titanium oxide or a strongly acidic sterilization zone, the inactivation takes a certain amount of time, and the effect is not necessarily sufficient.

  Patent Document 1 discloses a method for removing harmful substances in a gas phase atmosphere using a harmful substance removing material in which an antibody is supported on a carrier, wherein the carrier is a fiber having an official moisture content of 7% or more. A method for removing harmful substances, characterized in that it is formed by the method described above. However, the harmful substance removing material described in Patent Document 1 has a problem that the preservability of antibodies is not sufficient.

  On the other hand, in Patent Document 2, two or more materials selected from a material having antiallergenic properties, a material having antibacterial properties, a material having antiviral properties, and a material having antifungal properties are attached. A characteristic air purifying filter is described, and the above antibacterial materials include inorganic compounds that elute metal ions such as silver, copper, and zinc, fine metal particles of silver, copper, and zinc, iodine compounds, and phenols. , Quaternary ammonium salts, imidazole compounds, benzoic acids, hydrogen peroxide, cresol, chlorhexidine, irgasan, aldehydes, sorbic acid, and other drugs, lysozyme, cellulase, protease and other enzyme preparations, catechins, bamboo extract, Natural ingredient extracts such as hinoki extract, wasabi extract, mustard extract, etc. can be used, and the above anti-virus As a material having a gender, an inorganic compound which elutes the metal ions such as silver, copper, zinc, silver, copper and zinc metal particles, a lower alcohol, catechins, it is described that such a use hinokitiol.

Japanese Patent No. 3642340 JP 2005-7346 A

  This invention made it the subject which should be solved to eliminate the problem of the conventional hazardous | toxic substance removal material. That is, the present invention efficiently captures harmful substances derived from microorganisms such as bacteria and viruses, quickly inactivates them to minimize the effects on the human body, and has good antibody storage and a long life. Providing a toxic substance removal material possessed was an issue to be solved. Moreover, this invention made it the problem which should be solved to provide the efficient harmful substance removal method using the said hazardous substance removal material.

  As a result of intensive studies to solve the above problems, the present inventors have captured the harmful substance efficiently by supporting the antibody and the antioxidant substance on the carrier, quickly inactivating them, and affecting the human body. The inventors have found that it is possible to provide a harmful substance removing material that has a long life and has a good antistorability and light resistance of antibodies, and has completed the present invention.

That is, according to the present invention, there is provided a harmful substance removing material comprising a carrier on which an antibody and an antioxidant substance are supported.
Preferably, the antioxidant comprises at least one antioxidant vitamin.

Preferably, the antioxidant comprises at least one polyphenol.
Preferably, the antioxidant comprises at least one carotenoid.
Preferably, the antioxidant comprises at least either lycopene or astaxanthin.
Preferably, the antioxidant substance is carried on the carrier as an emulsion.
Preferably, the average particle size of the emulsion is 500 nm or less.

Preferably, the antioxidant emulsion and the antibody are mixed and dispersed in the same layer on the carrier.
Preferably, at least one hydrophilic polymer is further supported on the carrier.
Preferably, the antibody is an ostrich-derived antibody.

According to the present invention, there is further provided a method for removing harmful substances, which comprises removing harmful substances in the gas phase or liquid phase using the above-mentioned hazardous substance removing material of the present invention.

  According to the present invention, it is possible to remarkably improve storage stability and light resistance by supporting an antibody and an antioxidant on a carrier, and to provide a harmful substance removing material having a long life. became. According to the method of the present invention, an air cleaner or a liquid cleaner that can efficiently remove harmful substances in a gas phase or a liquid phase can be produced, which is very useful in the industry.

Hereinafter, the present invention will be described in more detail.
The hazardous substance removing material of the present invention is characterized by comprising a carrier on which an antibody and an antioxidant substance are supported.

(1) Carrier The main material forming the carrier used in the present invention is preferably a fiber mainly composed of at least one of cellulose ester, vinylon, acrylic and polyurethane. Further, as a main material for forming the carrier, fibers mainly composed of polyamide are also preferable. The main component as used in the field of this invention refers to the component which comprises 25% or more in the mass fraction in all the fibers.

  The cellulose ester in the present invention refers to a cellulose derivative in which the hydroxyl group of cellulose is esterified with an organic acid. Examples of organic acids used for esterification include fatty carboxylic acids such as acetic acid, propionic acid and butyric acid, and aromatic carboxylic acids such as benzoic acid and salicylic acid. It may be used alone or in combination. Although there is no restriction | limiting in particular about the ester group substitution rate of the hydroxyl group of a cellulose, It is preferable that it is 60% or more.

  Among the main group of materials forming the carrier in the present invention, cellulose acylate fibers are desirable. Cellulose acylate refers to a cellulose ester in which some or all of the hydrogen atoms constituting the hydroxyl group of cellulose are substituted with acyl groups. Examples of the acyl group include an acetyl group, a propionyl group, and a butyryl group. These groups may be constituted by replacing only one kind, or two or more kinds of acyl groups may be mixed and substituted. The total acyl group substitution degree is preferably 2.0 to 3.0, more preferably 2.1 to 2.8, and particularly preferably 2.2 to 2.7. Among these, cellulose acetate, cellulose acetate propionate, or cellulose acetate butyrate that satisfies this degree of substitution is preferable, and cellulose acetate is most preferable. Cellulose acylate is generally known to have different solvents depending on the degree of esterification, but after preparing a carrier with cellulose acylate having a high esterification rate in advance, the surface is hydrophilized by alkali hydrolysis treatment or the like. May be.

  Although it is possible to form a sufficiently practical harmful substance removal material using only cellulose acylate fibers, polyester fibers, polyolefin fibers, polyamides are used for the purpose of further improving strength and dimensional stability. The carrier may be formed of a blended fiber such as a fiber or an acrylic fiber. When blended fiber is used, the mass fraction of the cellulose acylate fiber is preferably 50% or more, and more preferably 70% or more.

  Of the main group of materials forming the carrier in the present invention, it is also desirable to be a polyamide fiber.

  The polyamide in the present invention refers to a fiber made of a linear polymer having an amide bond in a chemical structural unit.

  Among polyamides, a linear chain that is a combination of an aliphatic diamine such as ethylenediamine, 1-methylethylenediamine, 1,3-propylenediamine, and hexamethylenediamine and an aliphatic dicarboxylic acid such as malonic acid, succinic acid, and adipic acid. Type aliphatic polyamides are preferred. Nylon 66 is particularly preferable.

  Fats using lactams such as ε-caprolactam and laurolactam, aminocarboxylic acids such as aminocaproic acid and aminoundecanoic acid, para-aminomethylbenzoic acid and the like alone or as a copolymer component in addition to the diamine and dicarboxylic acid. A group polyamide can also be used. In particular, nylon 6 produced by using ε-caprolactam alone is preferable.

  In addition to these, some or all of the aliphatic diamines used as raw materials are alicyclic diamines such as cyclohexanediamine, 1,3-bis (aminomethyl) cyclohexane, and 1,4-bis (aminomethyl) cyclohexane. An aliphatic polyamide using an alicyclic dicarboxylic acid such as 1,4-cyclohexanedicarboxylic acid, hexahydroterephthalic acid, hexahydroisophthalic acid, etc. may be used as an aromatic polyamide and / or a part or all of the dicarboxylic acid. .

  Furthermore, by using aromatic diamines such as aliphatic paraxylylenediamine (PXDA) and metaxylylenediamine (MXDA), and aromatic dicarboxylic acids such as terephthalic acid as partial raw materials, water absorption is reduced and elastic modulus is improved. Also included is a polyamide that achieves the above. Further, it may be a polymer having an amide bond in a side chain such as polyacrylic acid amide, poly (N-methylacrylic acid amide), poly (N, N-dimethylacrylic acid amide) and the like.

Most preferred of the polyamides is nylon 66 or nylon 6. Appropriate hygroscopicity derived from amide bonds, easy to orient the molecular chain consisting of long chain fatty acids of appropriate length, relatively high stretchability, high heat of fusion and large heat capacity It is difficult to melt in terms of kinetics (melt resistance), the flexibility of molecular chains consisting of long-chain fatty chains, and the property that fibrillation and kink bands do not easily occur due to the formation of hydrogen bonds between amide bonds. This is because the properties preferable for the carrier of the present invention such as flexibility can be utilized.
A polyamide having an amide bond in the chemical structural unit in the side chain instead of the main chain can also be preferably used. Examples thereof include polyacrylamides such as poly (N-isopropylacrylamide), poly (N, N′-dimethylacrylamide), and poly (N-hexylacrylamide). In general, polymers with amide bonds in the side chains are highly hydrophilic and easily swell and deform, so they are hydrophobized by methods such as forming physical crosslinks or introducing alkyl groups using gelation. It is desirable.

  Similarly, for the purpose of improving strength and dimensional stability, the carrier may be reinforced with other appropriate structural materials such as metal, polymer material, ceramics and the like. These reinforcing materials are desirably used for portions other than the substantially outermost surface of the side surface to which the harmful substance removing material is supplied (for example, used on the opposite surface of the side surface or a core material).

  The vinylon in the present invention is a fiber composed of a linear polymer containing 65% by mass or more of vinyl alcohol units, and having a moisture content of less than 7% after being left for 1 week or longer in an environment of temperature 20 ° C. and humidity 65%. . It may be a formalized hydroxyl group of vinyl alcohol, but it is a non-formalized fiber that has been subjected to water resistance treatment by a polymer such as a boric acid-crosslinked hydroxyl group or a known alkali spinning method or cooling gel spinning method. It may be. Components other than vinyl alcohol units may include ethylene chains, vinyl acetate chains, etc., but fibers formed from vinyl alcohol carriers are preferred. Furthermore, it is most desirable to be a non-formalized fiber by cooling gel spinning in that it is homogeneous and has a high degree of orientation and crystallinity, so that excellent mechanical properties and reliability can be obtained.

  Vinylon generally has high strength, high elastic modulus, moderate hydrophilicity, weather resistance, chemical resistance, adhesion and the like with respect to other fibers, and utilizes these preferable performances as a carrier of the present invention. be able to.

  The acrylic system in the present invention refers to a fiber containing 40% or more of acrylonitrile group repeating units by mass ratio, for example, a homopolymer of acrylonitrile, a nonionic monomer such as an acrylate ester, a methacrylate ester, or vinyl acetate; Examples include copolymers of acrylonitrile, copolymers of anionic monomers such as vinylbenzene sulfonic acid and allyl sulfonic acid and acrylonitrile, and copolymers of cationic monomers such as vinyl pyridine and methyl vinyl pyridine and acrylonitrile. So-called promix fibers formed from acrylonitrile and milk casein are also included in this category.

  In general, acrylic fibers are often produced by an organic wet spinning method. In this method, when the spinning stock solution forms a coagulated yarn in the coagulation bath, water as a coagulant enters the spinning stock solution spun from the nozzle, while the spinning solvent diffuses from the spun stock solution to the outside. At this time, the water and the organic solvent (DMF, DMAc, etc.) are mutually diffused, so that a polymer is precipitated and a coagulated yarn having a structure in which innumerable cavities are connected in a network form is formed. In addition, it is characterized by deformation of the fiber cross section formed by volume shrinkage due to diffusion of the solvent into the coagulation bath during the coagulation process and formation of irregularities by forming a macrofibril structure on the surface. These fine structures are preferable as the structure of the carrier used in the present invention in terms of improving the specific surface area and ease of carrying the antibody.

  The acrylic fiber used in the present invention varies depending on the composition of the raw material polymer, the spinning method, the post-treatment conditions in the production process, etc., but generally, it is easy to obtain a bulky fiber with moderate hydrophilicity and high weather resistance. There are advantages.

  The polyurethane used in the present invention refers to a fiber composed of a linear synthetic polymer in which the bonding portion between monomers or the bonding portion between basic base polymers is mainly a urethane bond. It is desirable that the polyurethane segment contains 85% or more by mass ratio. It is desirable to be a block copolymer of a segmented polyurethane composed of a soft segment having a low melting point and a soft molecular weight of up to several thousand, and a hard segment having a high melting point and rigidity and high cohesion. Polyethers such as polypropylene glycol and polytetramethylene glycol can be used as the soft segment, and urethane groups formed from 4,4'-diphenylmethane diisocyanate, m-xylene diisocyanate, and the like can be used as the hard segment. Polyurethane is generally characterized by high elasticity. It varies depending on the temporary structure of the polymer chain, such as the chemical structure and distribution of both segments, and on the difference in secondary structure resulting from differences in the spinning conditions, but it stretches well. It has characteristics such as high resilience, resistance to aging compared to rubber materials, and thin fibers can be obtained, and these properties can be utilized even when used as a carrier of the present invention.

  Regarding the mechanical properties and dimensional stability of the fibers constituting the carrier, it is desirable that the elongation at drying is 25% or more. Here, the elongation at drying refers to the breaking elongation in a tensile test at 20 ° C. of the fiber dried over a sufficiently long time. In general, it is preferable that the elongation at drying is 10% or more and that it is suitable for processing such as cloth making is 25% or more in order to prevent filter processing and breakage during practical use (leading to a decrease in filtration efficiency). % Or more is more preferable, and 35% or more is most preferable.

  The official moisture content of the fibers constituting the carrier is preferably 1.0% or more and less than 7.0%, more preferably 3.0% or more and less than 6.5%, and more preferably 5.0% or more and 6% or less. Most preferably, it is less than 5%. At the official moisture content in this area, the activity of the supported antibody can be expressed, and the carrier's mechanical strength, rigidity, and dimensional stability with respect to the environment (especially humidity) should be obtained. Can do.

  The moisture content referred to here is the official moisture content, and the official moisture content is the moisture content contained in the fiber when the fiber is left in an environment of 20 ° C. and a relative humidity of 65% for a long time. Point to. In the case of a blended fiber with other fibers, the official moisture content of the entire blended fiber is indicated.

  The surface of the fiber constituting the carrier preferably has a fine concavo-convex structure on the scale of several tens of nanometers to several micrometers. The shape of the irregularities may be a three-dimensional shape such as a groove or streak formed in a direction parallel to the fiber direction, or a groove or streak formed perpendicular to the fiber direction, that is, concentrically with respect to the axis. Three-dimensional shapes may be used, and these three-dimensional shapes formed at an arbitrary angle from the direction parallel to the fiber direction to the vertical direction may exist at an arbitrary ratio and density. Samples obtained by the known cellulose acetate fiber spinning method are known to form a chrysanthemum shape with an indefinite fiber cross section due to the formation of a skin layer on the surface layer and the depression of the skin layer accompanying solvent drying. This unevenness is also a preferred form in the present invention.

  The fine concavo-convex structure on the nanometer to micrometer scale may be a hole and / or a protrusion. The average diameter is preferably 50 nm to 1 μm of holes or protrusions. These vacancies and protrusions are formed in a spinning process by a method such as using a solution in which cavitation of a solution or a fine dispersoid is dispersed (for example, mixing with a slurry in which barium sulfate particles are dispersed), It can be formed in a subsequent step by a method such as hydrolysis of the group or surface oxidation treatment (for example, the surface of the fiber is celluloseized with an aqueous alkali solution and then the microcrater is expressed on the fiber surface by enzyme treatment).

  The average fiber diameter of the fibers used in the hazardous substance removing material of the present invention is preferably 50 μm or less, more preferably 10 μm or less, particularly preferably 1 μm or less, and most preferably 100 nm or less. preferable. In addition, the average fiber diameter of this invention is a numerical value calculated | required by measuring the diameter in the fiber in arbitrary 300 places from the observation image of a scanning electron microscope (SEM), and arithmetically averaging it.

  The fiber used in the present invention can be produced by a general production method such as melt spinning, wet spinning, dry spinning, wet drying spinning, or physical treatment (for example, strong mechanical shearing treatment using an ultra-high pressure homogenizer). In order to ensure stable quality, it is preferable to use dry spinning or wet dry spinning. In order to produce uniform fibers with an average fiber diameter of 100 nm or less, further processing techniques, 2005, 40, No. 2, 101, and 167; Polymer International, 1995, 36, 195- 201; Polymer Preprints, 2000, 41 (2), 1193; Journal of Macromolecular Science: Physics, 1997, B36, 169, etc. It is preferable to employ the electrospinning method.

  Solvents used for spinning include chlorinated solvents such as methylene chloride, chloroform and dichloroethane, amide solvents such as dimethylformamide, dimethylacetamide and N-methylpyrrolidone, and ketones such as acetone, ethyl methyl ketone, methyl isopropyl ketone and cyclohexanone. Any solvent that dissolves the resin used for the synthetic resin fiber, such as a solvent, an ether solvent such as THF or diethyl ether, or an alcohol solvent such as methanol, ethanol, or isopropanol can be used. These solvents may be used alone or in combination of two or more.

  When the electrospinning method is employed, a salt such as lithium chloride, lithium bromide, potassium chloride, or sodium chloride may be further added to the resin solution.

It is desirable that the fibers constituting the carrier of the harmful substance removing material of the present invention have a structure in which a three-dimensional network is formed by partial adhesion. By adopting such a structure, it is possible to improve processing and practical mechanical resistance, and to improve the reliability of the hazardous substance removing material. In addition, the retention characteristics of the antibody of the present invention can be improved. The adhesion between fibers can be observed by a method such as SEM. The density of the bonding points between the fibers is preferably 10 or more per 1 mm square with respect to the projected surface area of the harmful substance removing material, and more preferably 100 or more.

  As a method for forming an adhesion point, it may be formed by an adhesion formed by a dry spinning method or a fusion point formed by a melt spinning method, or after spinning, addition of an adhesive, a plasticizing solvent, etc. You may perform the adhesion point formation process by. From the viewpoint of production cost, it is preferable to form adhesion points by a dry spinning method using an appropriate solution formulation.

(2) Antioxidant As an example of the antioxidant used in the present invention, antioxidant vitamins can be used. Antioxidant vitamins refer to vitamins that have the ability to scavenge active oxygen. Some are fat-soluble and others are water-soluble.

  Fat-soluble vitamins include vitamin E, vitamin A, vitamin K, vitamin D, and their homologues, isomers, derivatives, precursors and the like. The origin may be natural or synthetic. Specifically, in vitamin E, α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol homologs, and RRR-α-tocopherol Isomers such as 2-epi-α-tocopherol and 2-ambo-α-tocopherol, vitamin E derivatives such as tocopherol acetate, tocopherol calcium succinate, and dl-α-tocopherol acetate. In vitamin A, retinol, retinal, retinoic acid, 3-dehydroretinol, 3-dehydroretinal, 3-dehydroretinoic acid, or provitamin A, β-carotene, α-carotene, γ-carotene, β- Examples include cryptoxanthin, ecainenone, β-apo-12′-carotenal, β-apo-12′-carotene acid and the like. As for vitamin K, in addition to phylloquinone (K1), menaquinone (K2) homologue, etc., menadione (K3), menadiol (K4), 4-amino-2-methyl-1-naphthol (K5) combined by synthesis ), 2-methyl-1,4-naphthalenediamine (K6), 4-amino-3-methyl-1-naphthol (K7) and the like. For vitamin D, vitamin D2, vitamin D3, vitamin D4, vitamin D5, vitamin D6, vitamin D7, provitamin D such as ergosterol and 7-DHC, previtamin D, pyrovitamin D, isopyrovitamin D, etc. Isomers and the like. The form is not limited to any liquid, paste, granule, powder or the like. Among these, vitamin E or a derivative thereof, or a combination thereof is particularly preferable. Further, α-tocopherol or a derivative thereof, or a combination thereof is more preferable.

  In the present invention, these can be mixed and used, but at least one is preferably vitamin E. Vitamin E includes α, β, γ, δ, each tocopherol or each tocotrienol, optical isomers thereof, or tocopherol derivatives such as tocopherol acetate, and preferably includes one or more of these. The origin is not limited to animal raw materials, plant raw materials or the like, and the form is not limited to liquid, paste, granule, powder or the like.

  Examples of the water-soluble vitamin include ascorbic acid or a derivative thereof, erythorbic acid or a derivative thereof. Ascorbic acid or ascorbic acid derivatives or salts thereof include L-ascorbic acid, L-ascorbic acid Na, L-ascorbic acid K, L-ascorbic acid Ca, L-ascorbic acid phosphate, L-ascorbic acid phosphate Magnesium salt, L-ascorbic acid sulfate, L-ascorbic acid sulfate disodium salt, L-ascorbic acid stearate, L-ascorbic acid 2-glucoside, L-ascorbyl palmitate, tetraisopalmitate L -Ascorbyl and the like. Among these, L-ascorbic acid, L-ascorbic acid Na, L-ascorbic acid stearate, L-ascorbic acid 2-glucoside, L-ascorbyl palmitate, magnesium salt of L-ascorbic acid phosphate, L-ascorbic acid sulfate disodium salt and L-ascorbyl tetraisopalmitate are particularly preferred. As erythorbic acid or erythorbic acid derivatives or salts thereof, erythorbic acid, erythorbic acid Na, erythorbic acid K, erythorbic acid Ca, erythorbic acid phosphate ester, erythorbic acid sulfate ester, erythorbic acid palmitic acid ester, erythorbyl tetraisopalmitate, etc. Is mentioned. Of these, erythorbic acid and erythorbic acid Na are particularly preferred.

  As an example of the antioxidant used in the present invention, carotenoids can be used. Examples of carotenoids that can be used in the present invention include astaxanthin, α-carotene, β-carotene, γ-carotene, rutin, cryptoxanthine, zeaxanthin, crocetin, paprika pigment, anato, gardenia yellow pigment, lycopene pigment, and the like. More preferred are esters thereof. Astaxanthin is widely distributed in the animal and plant kingdoms in nature, and is mainly used as a coloring agent for farmed fish and poultry. Astaxanthin is known to have an antioxidant effect, an anti-inflammatory effect, a skin aging prevention effect, a whitening effect, and the like.

Astaxanthin is a red pigment having an absorption maximum at 476 nm (ethanol) and 468 nm (hexane) and belongs to a kind of carotenoid xanthophylls. The chemical structure of astaxanthin is 3,3′-dihydroxy-β, β-carotene-4,4′-dione (C ₄ OH ₅₂ O ₄ , molecular weight 596.82), and the chemical formula is represented by the following general formula (1). It is.

  Astaxanthin used in the present invention is preferably a pigment derived from Haematococcus alga. It is known that a pigment derived from Haematococcus alga is different from a pigment derived from krill and synthesized astaxanthin.

  Specific examples of the raw material for the Haematococcus alga pigment used in the present invention include Haematococcus pluviaris, Haematococcus lacustris, Haematococcus capescoscato, (Haematococcus drobakensis), Haematococcus zimbabiensis and the like.

  As a method for culturing Haematococcus alga used in the present invention, various methods disclosed in literature [Japanese Patent Laid-Open No. 8-103288 (Claims, paragraphs [0001] to [0008])] and the like are adopted. However, it is not particularly limited as long as the morphological change is made from a vegetative cell to a cyst cell that is a dormant cell.

  As for the pigment derived from Haematococcus alga used in the present invention, the above-mentioned raw materials can be used, for example, in literature [Japanese Patent Laid-Open No. 5-68585 (Claims, paragraphs [0001] to [0005])] and the like. The cell wall is crushed by the disclosed method and obtained by adding an organic solvent such as acetone, ether, chloroform and alcohol (ethanol, methanol, etc.) or an extraction solvent such as supercritical carbon dioxide. Moreover, what is marketed widely can be used, for example, ASTOTS-S, -2.5 O, -5 O, -10 O, etc. made by Takeda Shiki Co., Ltd., Fuji Chemical Industry Co., Ltd. And Asteryl Oil 50F, 5F, etc. manufactured by Toyo Enzyme Chemical Co., Ltd. and the like.

  As an example of the antioxidant used in the present invention, polyphenol can be used. Polyphenols include flavonoids (catechin, anthocyanin, flavone, isoflavone, flavan, flavanone, rutin), phenolic acids (chlorogenic acid, ellagic acid, gallic acid, propyl gallate), lignans, curcumins, coumarins, etc. be able to. Moreover, since these compounds are contained in a large amount in the following natural product-derived extracts, they can be used in the form of extracts. For example, licorice extract, cucumber extract, quette extract, gentian (gentian) extract, Gentian extract, cholesterol and its derivatives, hawthorn extract, peonies extract, ginkgo biloba extract, sorghum (ogon) extract, carrot Extract, Maika (Maika, Hamanasu) extract, Sunpens (Kawara-Ketsumei) extract, Tormentilla extract, Parsley extract, Button (buttonpi) extract, Mokka (bokeh) extract, Melissa extract, Yashajitsu (Yasha) extract , Yukinoshita extract, Rosemary (mannenrou) extract, lettuce extract, tea extract (Oolong tea, black tea, green tea, etc.), microbial fermentation metabolites, Rakan fruit extract, etc. (in parentheses are plant aliases) , Herbal medicine name etc. were described.) Among these polyphenols, catechin, rosemary extract, glucosyl rutin, ellagic acid, and gallic acid are particularly preferable.

  As the polyphenol, a commercially available product can be appropriately used. For example, ellagic acid (Wako Pure Chemicals, etc.), rosemary extract (trade names RM-21A, RM-21E: Mitsubishi Chemical Foods, etc.), catechin (trade names Sancatol W-5, No. 1: Taiyo Kagaku, etc.) , Na gallate (trade name: Sancatol: Taiyo Kagaku, etc.), rutin, glucosylrutin, enzymatically-degraded rutin (trade names: Rutin K-2, P-10: Kiriya Chemical, trade name: αG rutin: Hayashibara Biochemical Research Institute, etc. ) Etc.

  In the present invention, the antioxidant substance is preferably carried on a carrier as an emulsion. The average particle size of the emulsion is generally preferably 1 nm to 1000 nm, more preferably 1 nm to 500 nm, further preferably 10 nm to 500 nm, further preferably 10 nm to 300 nm, and more preferably 10 nm to 200 nm. Particularly preferred. The particle size of the emulsion can be measured with a particle size distribution meter or the like.

  Further, the content of the antioxidant substance in the emulsion is not particularly limited as long as the effect of the present invention can be achieved, but is generally 0.01% by weight or more and 10% by weight or less, preferably 0.1% by weight. % To 5% by weight.

  The emulsion in the present invention can be prepared by a known emulsion preparation method. Details are described in "Emulsification / Solubilization Technology" (Tatsumi, published by Kogyosho Co., Ltd.), pages 65-66 and 92-105, and both the aggregation method and the dispersion method are preferably used. . In addition, as described on pages 88-90 of "Emulsifier for Foods 2nd Edition" (published by Hidaka, published by Sachishobo), (1) self-emulsification method, (2) soap production method, (3) simple emulsification method, Although any method classified as (4) transfer emulsification method and (5) surfactant method emulsification method is preferable, the simple emulsification method or surfactant method emulsification method is particularly suitable for the preparation of high-concentration emulsions. The surfactant method emulsification method is preferable.

The emulsion in the present invention can be prepared using an emulsifier, and a water-soluble emulsifier is preferred as the emulsifier. The water-soluble emulsifier is not particularly limited as long as it is an emulsifier that dissolves in an aqueous medium. For example, a nonionic surfactant having an HLB of 10 or more, preferably 12 or more is preferable. If the HLB is too low, the emulsifying power may be insufficient. Here, HLB is a hydrophilic-hydrophobic balance usually used in the field of surfactants, and a commonly used calculation formula such as the following formula can be used.
HLB = 7 + 11.7log (Mw / M0)
Here, Mw is the molecular weight of the hydrophilic group, and M0 is the molecular weight of the hydrophobic group. Moreover, you may use the numerical value of HLB described in the catalog etc. Furthermore, as can be seen from the above formula, an emulsifier having an arbitrary HLB value can be obtained by utilizing the additivity of HLB.

  The emulsifier that can be used in the present invention is not particularly limited, but a nonionic emulsifier is preferable. Examples of the nonionic emulsifier include glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleic acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, and lecithin. More preferred are polyglycerin fatty acid esters, sorbitan fatty acid esters, and sucrose fatty acid esters. The emulsifier is not necessarily highly purified by distillation or the like, and may be a reaction mixture.

  The polyglycerin fatty acid ester used in the present invention has an average degree of polymerization of 2 or more, preferably 6 to 15, more preferably 8 to 10 and fatty acid having 8 to 18 carbon atoms, such as caprylic acid and caprin. Esters with acids, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, and linoleic acid. Preferred examples of polyglycerol fatty acid esters include hexaglycerol monooleate, hexaglycerol monostearate, hexaglycerol monopalmitate, hexaglycerol monomyristate, hexaglycerol monolaurate, decaglycerol monooleate , Decaglycerin monostearic acid ester, decaglycerin monopalmitic acid ester, decaglycerin monomyristic acid ester, decaglycerin monolauric acid ester and the like. These polyglycerin fatty acid esters can be used alone or in combination.

  Examples of commercially available products include Nikko Chemicals, Inc., NIKKOL DGMS, NIKKOL DGMO-CV, NIKKOL DGMO-90V, NIKKOL DGDO, NIKKOL DGMIS, NIKKOL DGTI, NIKOL DGTIS, NIKKOL DGTI Tetraglyn 3-S, NIKKOL Tetraglyn 5-S, NIKKOL Tetraglyn 5-O, NIKKOL Hexaglyn 1-L, NIKKOL Hexaglyn 1-M, NIKKOL Hexaglyn 1-SV, NIKKOL Hexaglyn 1-O, NIKKOL Hexaglyn 3-S, NIKKOL Hexaglyn 4 -B, NIKKOL Hexaglyn 5-S, NIKKOL Hexaglyn 5-O, NIKKOL Hexaglyn PR-15, NIKKOL Decaglyn 1-L, NIKKOL Decaglyn 1-M, NIKKOL Decaglyn 1-SV, NIKKOL Decaglyn 1-50SV, NIKKOL Decaglyn 1-ISV, NIKKOL Decaglyn 1-O, NIKKOL Decaglyn 1 -OV, NIKKOL Decaglyn 1-LN, NIKKOL Decaglyn 2-SV, NIKKOL Decaglyn 2-ISV, NIKKOL Decaglyn 3-SV, NIKKOL Decaglyn 3-OV, NIKKOL Decaglyn 5-SV, NIKKOL Decaglyn 5-HS, NIKKOL Decaglyn 5-IS , NIKK L Decaglyn 5-OV, NIKKOL Decaglyn 5-O-R, NIKKOL Decaglyn 7-S, NIKKOL Decaglyn 7-O, NIKKOL Decaglyn 10-SV, NIKKOL Decaglyn 10-IS, NIKKOL Decaglyn 10-OV, NIKKOL Decaglyn 10-MAC, NIKKOL Decaglyn PR-20, Ryoto polyglycerate L-10D, L-7D, M-10D, M-7D, P-8D, S-28D, S-24D, SWA-20D, manufactured by Mitsubishi Chemical Foods, Inc. SWA-15D, SWA-10D, O-50D, O-15D, B-100D, B-70D, ER-60D, Taiyo Chemical Co., Ltd. Sunsoft Q-17UL, Sunsoft Q-14S, Sanso Preparative A-141C, manufactured by Riken Vitamin Co., Ltd. Poem DO-100, and the like Poem J-0021.

  The sorbitan fatty acid ester used in the present invention preferably has a fatty acid having 8 or more carbon atoms, more preferably 12 or more. Preferred examples of the sorbitan fatty acid ester include sorbitan monocaprylate, sorbitan monolaurate, sorbitan monostearate, sorbitan tristearate, sorbitan tristearate, sorbitan isostearate, sorbitan sesquiisostearate, sorbitan oleate, sorbitan sesquioleate And sorbitan trioleate. These sorbitan fatty acid esters can be used alone or in combination. As a commercial item, Nikko Chemicals Co., Ltd. make, NIKKOL SL-10, SP-10V, SS-10V, SS-10MV, SS-15V, SS-30V, SI-10RV, SI-15RV, SO-15 10V, SO-15MV, SO-15V, SO-30V, SO-10R, SO-15R, SO-30R, SO-15EX, manufactured by Daiichi Kogyo Seiyaku Co., Ltd., Sorgen 30V, 40V, 50V, 90, 110 or the like.

  The sucrose fatty acid ester used in the present invention preferably has a fatty acid having 12 or more carbon atoms, more preferably 12-20. Preferred examples of sucrose fatty acid esters include sucrose dioleate, sucrose distearate, sucrose dipalmitate, sucrose dimyristic ester, sucrose dilaurate, sucrose monooleate, sucrose Examples thereof include sugar monostearate, sucrose monopalmitate, sucrose monomyristate, and sucrose monolaurate. In the present invention, these sucrose fatty acid esters can be used alone or in combination. Examples of commercially available products include Ryoto Sugar Esters S-070, S-170, S-270, S-370, S-370F, S-570, S-770, and S-970 manufactured by Mitsubishi Chemical Foods Corporation. , S-1170, S-1170F, S-1570, S-1670, P-070, P-170, P-1570, P-1670, M-1695, O-170, O-1570, OWA-1570, L -195, L-595, L-1695, LWA-1570, B-370, B-370F, ER-190, ER-290, POS-135, DK ester SS manufactured by Daiichi Kogyo Seiyaku Co., Ltd., F160, F140, F110, F90, F70, F50, F-A50, F-20W, F-10, F-A10E, Cosmelike B-30, S-10, S-50, S-70, S -110, S-160, S-190, SA-10, SA-50, P-10, P-160, M-160, L-10, L-50, L-160, L-150A, L-160A , R-10, R-20, O-10, O-150 and the like.

  The content of the emulsifier in the emulsion of the present invention is generally 0.001 to 20% by mass, preferably 0.01 to 10% by mass, more preferably 0.1 to 5.0% by mass.

(3) Hydrophilic polymer In the present invention, a hydrophilic polymer can be held on a carrier together with an antioxidant. The hydrophilic polymer that can be used in the present invention means a polymer having a hydrophilic functional group in the structure. The hydrophilic functional group is not particularly limited, but a polymer containing at least one of a hydroxyl group, amino group, amide group, carboxylic acid group, and quaternary amino group is preferable. Amino group, amide group, and quaternary amino group are preferable. Most preferred is a polymer having Examples of the polymer having a hydroxyl group include polyvinyl alcohol, polyethylene-polyvinyl alcohol copolymer, partially hydrolyzed polyvinyl acetate, partially substituted cellulose derivatives such as diacetyl cellulose, ethyl cellulose, and carboxymethyl cellulose. Further, it may be a natural product such as guar gum, pectin, starch, carrageenan, glucomannan, sialyl lactose, or a synthetic product thereof. Of these, polyvinyl alcohol is preferred. Examples of the polymer having an amino group include polyvinylamine and polyaminocaproic acid methacrylate. It may be a natural product such as chitosan or a synthetic product thereof. Of these, polyvinylamine is preferred.

  Examples of the polymer having an amide group include polyacrylamide and polyvinylpyrrolidone alone or a copolymer with a vinyl monomer such as (meth) acrylate or vinyl acetate. Further, it may be a natural product such as collagen, gelatin, fibroin, casein, keratin, casein or a synthetic product thereof (the amide group of the present invention includes an amide group constituting a peptide bond). Of these, polyacrylamide, polyvinylpyrrolidone and gelatin are preferred.

  Examples of the polymer having a polycarboxylic acid group include polyacrylic acid, carboxymethyl cellulose, and polylactic acid. Further, it may be a natural product such as alginic acid or hyaluronic acid or a synthetic product thereof. Of these, polyacrylic acid is preferred. Even if part or all of the carboxylic acid group is in a non-dissociated state, it may form a salt such as sodium, potassium or ammonium.

  Cationic polymers are also preferably used. A quaternary ammonium base is obtained by adding an alkyl halide or the like to an alkylamino group. Examples of specific monomers for deriving a structural unit having a quaternary ammonium group include N, N-dimethylaminoethyl (meth) acrylate / methyl chloride quaternized product, N, N-dimethylaminopropyl (meth) acrylamide / There are methyl chloride quaternized compounds and N, N-diallylmethylamine methyl chloride quaternized compounds. Examples of other cationic polymers include polydiallyldimethylammonium chloride, polyethyleneimine, polyvinylpyridine quaternary salt, and polymers having a quaternary phosphonium group. Further, cationic polymers such as these copolymers, dicyandiamide and formalin, and condensation products of alkylenediamine and epichlorohydrin are exemplified. Moreover, the betaine polymer | macromolecule which has these cationic groups and anionic groups, such as carboxylic acid, a sulfonic acid, and phosphonic acid, may be sufficient.

  The molecular weight of the hydrophilic polymer that can be used in the present invention can be arbitrarily set according to the type, purpose, type of supported antibody, etc., but generally the weight average molecular weight is preferably from 5,000 to 1,000,000. It is more preferably 10,000 or more and 500,000 or less, and most preferably 30,000 or more and 300,000 or less. The hydrophilic group content of the hydrophilic polymer of the present invention is preferably arbitrarily set depending on the type, purpose, supported antibody type, etc., and is preferably between 0.1 and 3 groups per monomer unit. More preferably, it is between 0.3 and 1.5, most preferably between 0.5 and 1. The polymer used for the coating may be used alone, or a plurality of polymers may be mixed or used as a copolymer with an arbitrary monomer. It is obvious to those skilled in the art that the hydrophilic polymer of the present invention should be selected from the viewpoint of affinity with not only the antibody but also the base material. That is, the preferred hydrophilic polymer varies depending on what is selected as the substrate. From this point of view, the hydrophilic polymer may be used by mixing a compound having a high affinity with the base material at an arbitrary ratio, or may be used after being copolymerized. The mixing ratio of the antioxidant and the hydrophilic polymer is in a mass ratio of 1: 1 to 1: 100, preferably 1: 1 to 1:20, and most preferably 1: 2 to 1:10.

(4) Antibody The antibody used in the hazardous substance removing material of the present invention is a protein that specifically reacts (antigen-antibody reaction) with a specific harmful substance (antigen), and has a molecular size of 7 to 8 nm. , Has a Y-shaped molecular form. Of the Y-shaped molecular structure of an antibody, a pair of branch parts is called Fab and a trunk part is called Fc, and among these, a toxic substance is captured at the Fab part.

  The type of the antibody corresponds to the type of harmful substance that can be captured. Examples of harmful substances captured by antibodies include bacteria, molds, viruses, allergens, and mycoplasmas. Specifically, examples of bacteria include gram-positive bacteria, Staphylococcus (S. aureus and Staphylococcus epidermidis), micrococcus, anthrax, cereus, Bacillus subtilis, acne, and gram-negative bacteria. And Pseudomonas aeruginosa, Serratia, Sephacia, Streptococcus pneumoniae, Legionella, and Mycobacterium tuberculosis. Examples of molds include Aspergillus, Penicillius, and Cladosporium. Examples of the virus include influenza virus, coronavirus (SARS virus), adenovirus, rhinovirus and the like. Examples of allergens include pollen, mite allergen, cat allergen and the like.

  In particular, in the present invention, an influenza antibody which is mainly a droplet infection and is a target of a harmful substance removal filter can be preferably used. As antigens used for preparing antibodies for influenza, various antigens of H1N1, H3N2, and B types, three mixed antigens, and H5 recombinant protein derived from avian influenza H5N1 can be used. Since H5 recombinant protein kills chickens, antibodies cannot be obtained in chicken eggs, but immunity is possible in ostriches.

  Examples of the method for producing the antibody include, for example, a method in which an antigen is administered to an animal such as a goat, horse, sheep, or rabbit, and a polyclonal antibody is purified from the blood, and a spleen cell and a cultured cancer cell of the animal to which the antigen is administered. Methods for purifying monoclonal antibodies from cell cultures and body fluids (such as ascites) of animals that have been transplanted with the cultures, purified antibodies from genetically modified bacteria, plant cells, or animal cell cultures into which antibody-producing genes have been introduced And a method of purifying ostrich egg antibodies or chicken egg antibodies from egg yolk powder obtained by administering antigens to ostriches or chickens to produce immunized eggs and sterilizing and spray drying egg yolk liquid. Among these, the method of obtaining an antibody from an ostrich or chicken egg can easily obtain a large amount of the antibody and can reduce the cost of the harmful substance removing material.

  The antibody used in the harmful substance removing material of the present invention is preferably an antibody produced from an ostrich or chicken egg. In particular, the ostrich antibody has a higher lipid content than the chicken antibody prepared under the same purification process, and is advantageous for co-loading with the antioxidant of the present invention.

  As an antibody produced from an ostrich egg, for example, those described in International Publication No. WO2007 / 026689 can be used. According to the method using ostrich eggs, it is possible to easily produce an antibody specific to a protein, which was difficult to produce by the conventional method, and to produce a uniform and large amount of antibody without lot difference. Ostrich means birds belonging to the order of Struthioniformes, and it is preferable to use ostriches (Struthio camelus) belonging to the family Struthionidae. An antibody produced from an ostrich egg can be produced according to the method described in paragraph numbers 0007 to 0034 of International Publication WO2007 / 026689.

  The carrier constituting the hazardous substance removing material of the present invention is subjected to antibacterial processing such as coating containing an antibacterial agent and / or antifungal processing such as coating containing a fungicide. desirable. Antibodies are basically proteins, especially ostrich egg antibodies are food, and may be accompanied by proteins other than antibodies, which are good food for bacteria and fungi to grow, If antibacterial processing and / or antifungal processing is applied, the growth of such bacteria and fungi is suppressed, and long-term storage can be performed.

  Examples of antibacterial / antifungal agents include organic silicon quaternary ammonium salts, organic quaternary ammonium salts, biguanides, polyphenols, chitosan, silver-supporting colloidal silica, and zeolite-supporting silver. The processing method includes impregnating or applying an antibacterial / antifungal agent to a fiber carrier, or a raw yarn raw cotton modified with an antibacterial / antifungal agent in the synthesis stage of the fibers constituting the carrier. There is a quality law.

  As a method for immobilizing an antibody on the carrier, the carrier is silanized using γ-aminopropyltriethoxysilane or the like, and then an aldehyde group is introduced onto the surface of the carrier with glutaraldehyde or the like. A method of covalent bonding, a method of immersing an untreated carrier in an antibody aqueous solution and immobilizing the antibody on the carrier by ionic bonding, an aldehyde group introduced into a carrier having a specific functional group, and the aldehyde group and the antibody A method of covalently bonding, a method of ionically binding an antibody to a carrier having a specific functional group, a method of introducing an aldehyde group after coating the carrier with a polymer having a specific functional group, and a method of covalently binding the aldehyde group and the antibody In the present invention, the antibody is simply supported on the carrier by spraying the solution in which the antibody is dissolved on the carrier. It can be.

The hazardous substance removing material of the present invention can be used for filters, masks, wipes and the like for air cleaners.
When used as a filter for an air purifier, any known filter such as a prefilter for removing coarse dust, a dust removal filter, a photocatalytic filter exhibiting a deodorizing effect, an antibacterial filter for removing other harmful substances, a VOC adsorption filter, etc. It may be used in combination with a filter.

  The features of the present invention will be described more specifically with reference to examples and comparative examples. The materials, amounts used, ratios, processing details, processing procedures, and the like shown in the following examples can be changed as appropriate without departing from the spirit of the present invention. Therefore, the scope of the present invention should not be construed as being limited by the specific examples shown below.

(Production of carrier)
Acetone: water (97: 3) solution (25% by mass) of cellulose acetate (manufactured by Aldrich, total substitution degree 2.4, number average molecular weight 30,000) is heated to 60 ° C., and from a nozzle having a diameter of 0.1 mm, A nonwoven fabric was formed by jetting with air at a spinning speed of 500 m / m to obtain a nonwoven fabric N-1 having a thickness of 85 μm. The spinning cylinder was heated to 100 ° C. with a heater. It was 8 micrometers when the average fiber diameter was measured by SEM.

(Antibody production)
Inactivated influenza virus (100 μg) antigen solution (0.5 mL) and complete adjuvant (0.5 mL) were mixed and inoculated into the ostrich chest muscle as the first immunization. From the second time, 0.5 mL of the same antigen solution and 0.5 mL of incomplete adjuvant were mixed and inoculated into the ostrich neck muscle every other week until the fourth week. Only egg yolk was collected from the egg laid by this ostrich and stirred. The egg yolk juice (10 mL) and TBS (20 mM Tris-HCL (pH 7.5), 0.15 M NaCL, 0.5% NaN) were mixed, and 5 mL of 10% dextran sulfate / TBS was added and stirred for 30 minutes. After adding 10 mL of 1M CaCl / TBS and stirring, the mixture was allowed to stand for 2 hours or more. The supernatant was collected by centrifugation at 10,000 rpm for 30 minutes. Ammonium sulfate was added to the supernatant to a final concentration of 40%, and the mixture was allowed to stand for 12 hours or more. Centrifugation was performed at 10,000 rpm, and the precipitate was recovered. This precipitate was resuspended in 10 mL of TBS and dialyzed with TBS.

(Emulsion preparation)
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose oleate (HLB = 15) 13g
・ Decaglyceryl monooleate (HLB = 12) 25g
・ Gelatin (MP Biomedical, derived from bovine skin) 20g
・ Pure water 802g

The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an oil phase composition.
・ Hematococcus alga extract (astaxanthin content 20% by mass) 40g
・ Lecithin (from soybean) 100g

  While maintaining the aqueous phase at 70 ° C., the mixture was stirred with a homogenizer (manufactured by SMT) (10000 rpm, 4 minutes), and the oil phase was added thereto to obtain an emulsion. The obtained emulsion was subjected to high-pressure emulsification at 200 MPa using an optimizer HJP-25005 (manufactured by Sugino Machine). Thereafter, the mixture was filtered through a microfilter having an average pore diameter of 1 μm to obtain an astaxanthin-containing emulsion E-1.

In addition, except that the Haematococcus alga extract was changed to the composition shown in Table 1, an antioxidant-containing emulsion E-2 to 9 and E-10 from which the antioxidant was removed were produced.
The average particle size of the emulsion was measured with a particle size distribution meter (LB-550: manufactured by HORIBA, Ltd.).

  Furthermore, antioxidant-containing emulsions E-11 to 13 were prepared in the same manner except that the rotation speed of the homogenizer was set to Table 2 and the high-pressure emulsification step was omitted.

(Filter sample preparation)
The dialysate is diluted with water to prepare an antibody concentration of 100 ppm. After uniformly mixing with the above emulsion 0.01% aqueous solution, 1 mL of the mixture is uniformly spread on a 10 cm ² non-woven fabric and allowed to stand at room temperature for 1 hour. Then, the filter sample was obtained by air drying at 40 ° C. for 2 hours (samples F-1 to 13).

  Sample F-14 prepared by the same method except that an astaxanthin suspension having an equal concentration was used instead of the above emulsion, and F-15 not using the emulsion were also prepared.

(Comparison with chicken-derived antibody)
Filter sample F-16 was prepared in the same manner as F-1, except that chickens were used instead of ostriches as immunized animals.

[Evaluation of virus inactivation efficiency]
The test virus solution was a purified influenza virus diluted 10-fold with PBS (200,000 plaques / mL). Each sample was cut into a 5 cm square and attached and fixed in the center of the virus spray test apparatus. The test virus solution was put in a nebulizer installed on the upstream side, and a virus recovery device was attached on the downstream side. Compressed air was sent from an air compressor, and the test virus was sprayed from the nebulizer spray port. A gelatin filter was installed on the downstream side of the sample, and air in the test apparatus was sucked at a suction flow rate of 10 L / min for 5 minutes to collect passing virus mist. After the test, the gelatin filter collecting the virus was collected, and the virus infectivity after passing through the sample was determined by the TCID50 method (50% cell infectious dose measurement method) using MDCK cells. From the comparison of the virus infectivity of the gelatin filter with and without the sample, the transient removal rate of the virus of each sample was calculated.

[High temperature and humidity preservability test]
The above virus spray test was performed before and after allowing F-1 to 13 to stand in an environment of 50% relative humidity 90% for one week. The results are shown in Table 3.

[Light resistance test]
For F-1, 9, 13, 14, and 15, the transient removal rate of the virus after exposure to a commercially available fluorescent lamp for 1 week at room temperature (100 mW / cm 2; 365 nm) was determined.

  About F-16: Since ostrich-derived antibodies have a characteristic of having more lipid components than chicken-derived antibodies, it is estimated that the effects of fat-soluble antioxidants can be easily obtained. Since the filter of the present invention also has improved light resistance, it is advantageous for use in combination with a photocatalyst using a UV lamp or a germicidal lamp, or for outdoor use (mobile type).

Claims (11)

A harmful substance removing material comprising a carrier on which an antibody and an antioxidant are supported. The hazardous substance removing material according to claim 1, wherein the antioxidant substance contains at least one antioxidant vitamin. The hazardous substance removing material according to claim 1, wherein the antioxidant substance contains at least one polyphenol. The hazardous substance removing material according to claim 1, wherein the antioxidant substance includes at least one kind of carotenoid. The hazardous substance removing material according to claim 1 or 4, wherein the antioxidant substance contains at least either lycopene or astaxanthin. The hazardous substance removing material according to any one of claims 1 to 5, wherein the antioxidant substance is supported on the carrier as an emulsion. The hazardous substance removing material according to claim 6, wherein the average particle size of the emulsion is 500 nm or less. The harmful substance removing material according to any one of claims 1 to 7, wherein the antioxidant emulsion and the antibody are mixed and dispersed in the same layer on the carrier. The hazardous substance removing material according to any one of claims 1 to 8, wherein at least one kind of hydrophilic polymer is further supported on a carrier. The hazardous substance removing material according to any one of claims 1 to 9, wherein the antibody is an ostrich-derived antibody. A method for removing harmful substances, comprising removing harmful substances in a gas phase or a liquid phase using the hazardous substance removing material according to claim 1.