JP2010018584A - COSMETIC COMPOSITION CONTAINING POLY-gamma-L-GLUTAMIC ACID AND/OR ITS SALT - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic and a skin preparation for external use blended with a poly-γ-L-glutamic acid and/or its salt and an antiaging agent, without having stickiness and excellent in refreshing feeling. <P>SOLUTION: This cosmetic composition without having the stickiness and excellent in the refreshing feeling contains the poly-γ-L-glutamic acid and/or its salt and the antiaging agent, while maintaining the effect of the antiaging agent. The antiaging agent may be an anti-inflammatory agent and/or an antioxidant. Further, the cosmetic composition may contain a humectant, an ultraviolet light absorbent and/or an anti-bacterial agent. The cosmetic composition can be utilized as a cosmetic, quasi-drug, medical supply or medicine. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof as an active ingredient, and particularly used for cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals. It is.

Although various aging phenomena appear in the skin, “anti-aging” is one of the major goals of skin care cosmetics. The skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin has a firm and glossy appearance and is kept fresh. However, aging, ultraviolet rays, drying, stress, etc. cause deterioration of the function of the extracellular matrix and fibroblasts in particular. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its tension and gloss and becomes rough. Aging symptoms such as wrinkles and dullness occur.

Currently, drugs with various anti-aging effects are being developed. For example, coenzyme Q10 is synthesized from acetyl CoA via mevalonic acid in living cells, and is distributed in large amounts in the heart, kidney, liver, muscle, pancreas, and thyroid gland. It has been reported that the amount of biosynthesis of coenzyme Q10 decreases with age, and the coenzyme Q10 concentration in human epidermis also decreases with age.
When coenzyme Q10 is absorbed into the body, most of it becomes reduced coenzyme Q10 and exhibits an antioxidant effect in cooperation with vitamin E. When applied continuously to human skin, the wrinkle is improved by suppressing oxidation. It is considered that the action mechanism is to suppress oxidative stress caused by ultraviolet rays and to suppress the expression of collagenase.

However, Patent Document 1 discloses water-solubilization of ubidecalenones with sucrose fatty acid esters as a method for producing a transparent aqueous solution containing ubidecalenones such as coenzyme Q10. However, the technique described in this example has failed to provide a cosmetic material that is satisfactory in terms of usability such as non-stickiness and freshness and in sustaining the effect.

On the other hand, biopolymers produced by microorganisms are promising. Among biopolymers, various functions have been found in a group of biopolymers called polyamino acids constituted by condensation polymerization of amino acids, and attention has been focused on their potential. Conventionally, three types of polyamino acids have been identified: poly-γ-glutamic acid (hereinafter referred to as “PGA”), poly-ε-lysine and cyanophysin.

  PGA is a polyamino acid in which an α-amino group and a γ-carboxyl group of glutamic acid are amide-bonded. PGA is a water-absorbing polyamino acid known as the main material of stringing of natto, which has been familiar to Japanese people for a long time, but as a background of this familiarity, it is due to its attractive functionality. large. As an attractive function of PGA, it is known that it has both biodegradability and high water absorption. Utilizing these functions, it is expected to be used in various fields and applications including the above-described cosmetics, medical products, foods and the like.

  Recently, it has been found that structural characteristics of polyamino acids (optical activity and type of constituent amino acids, molecular size, binding mode, etc.) are strongly reflected in their functionality. A well-known point is that it has both biodegradability and high water absorption. Utilizing these functions, it is expected to have various uses in many fields such as foods, cosmetics, and medical products. However, currently commercially available PGA is a chemically heterogeneous DL-PGA. Specifically, PGA is produced from Bacillus natto and its related bacteria, but D-glutamic acid and L-glutamic acid are irregularly bound, and the content ratio and sequence thereof are different for each culture of the producing bacteria. fluctuate. In general, the structural characteristics of polyamino acids (optical activity and type of constituent amino acids, molecular size, binding mode, etc.) strongly influence their functions. Since the DL-PGA has a different structure for each molecule, its property also differs for each molecule. This makes it difficult to stably produce DL-PGA having a desired quality.

  Bacteria that produce homopoly-γ-glutamic acid have also been reported. For example, Bacillus anthracis has been reported to produce poly-γ-D-glutamic acid (hereinafter sometimes referred to as D-PGA) consisting only of D-glutamic acid (Non-patent Document 1, Non-Patent Document 1, Patent Document 2, Non-Patent Document 3). However, since this bacterium is a bacterium having a strong pathogenicity, it is inappropriate as an industrial PGA-producing bacterium, and the molecular weight of D-PGA produced is small. In addition, it has been reported that the alkalophilic bacterium Bacillus halodurans produces poly-γ-L-glutamic acid and / or a salt thereof (hereinafter sometimes referred to as L-PGA) consisting only of L-glutamic acid (Non-Natural). Patent Document 3). However, L-PGA produced by this bacterium has an extremely small molecular weight, and is insufficient to obtain practical performance.

  On the other hand, it has been reported that the halophilic archaeon Natrialba aegyptiaca produces L-PGA having a molecular weight of about 100,000 to 1,000,000 as a high-molecular-weight homopoly-γ-glutamic acid-producing bacterium. However, since this bacterium has a molecular weight as small as about 100,000 under liquid culture conditions and hardly produces poly-γ-L-glutamic acid and / or a salt thereof, there is a problem as an industrially producing bacterium (non-patent) Document 4, Patent Document 2).

  In addition to the above, examples of organisms that produce L-PGA include hydra and the like, but hydra also has a problem that its molecular weight is extremely small. (Non Patent Literature 3)

    On the other hand, the present inventors made it possible to prepare a large amount of poly-γ-L-glutamic acid and / or a salt thereof with uniform optical purity and high molecular weight by liquid culture or the like. More specifically, poly-γ-L-glutamic acid and / or a salt thereof having a number average molecular weight of 1.3 million or more and uniform optical purity is obtained with a high productivity of 4.99 g or more per liter of culture solution. (Patent Document 3).

  Further, the method includes a cross-linking method and a cross-linked product of poly-γ-L-glutamic acid (Patent Document 3), and at least one of poly-γ-L-glutamic acid and poly-γ-L-glutamic acid cross-linked product. There is a report of an external preparation for skin (Patent Document 4).

Up to now, it has not been reported that L-PGA can be used in combination with an anti-aging agent in terms of usability such as non-stickiness and freshness to the skin without impairing its anti-aging effect.

JP 2004-115490 A Special Table 2002-517204 (announced June 18, 2002) JP2007-314434 JP2008-120725 Handy, W. E., and H.N.Rydon, Biochem J., 40, 297-309 (1946) Makino, S., I. Uchida, N. Terakado, C. Sasakawa, and M. Yoshikawa, J. Bacteriaol., 171, 722-730 (1989) Biology and Chemistry Vol.40, No.4, p212-214 (2002) Hezayen, F. F., B. H. A. Rehm, B. J. Tindall and A. Steinbuchel, Int. J. Syst. E., 51, 1133-1142 (2001)

  Since various aging phenomena appear in the skin, anti-aging agents are blended in various cosmetics as skin care cosmetics. However, cosmetics excellent in usability such as non-stickiness and freshness have been desired.

  An object of the present invention is to provide a cosmetic and a skin external preparation containing poly-γ-L-glutamic acid and / or a salt thereof and an anti-aging agent which are excellent in refreshing feeling and solve the above problems. .

Furthermore, the objective of this invention is providing the cosmetics, quasi-drug, medical supplies, hygiene articles, and a pharmaceutical containing the said cosmetic composition.

  As a result of diligent efforts to achieve the above object, the present inventors have found that poly-γ-L-glutamic acid and / or a salt thereof is effective.

  Furthermore, the present inventors have made extensive research efforts to obtain a skin external preparation that is non-sticky and has a refreshing feeling in an external preparation containing an anti-aging agent. -By using glutamic acid and / or a salt thereof, it was found that the stability of the anti-aging agent is improved and the effect thereof can be maintained, and it is not sticky and has a refreshing feeling, thereby completing the present invention.

That is, the present invention includes the following inventions (1) to (9).
(1) A cosmetic composition comprising poly-γ-L-glutamic acid and / or a salt thereof, and an anti-aging agent.
(2) The cosmetic composition according to 1, wherein the anti-aging agent is an anti-inflammatory agent and / or an antioxidant.
(3) The cosmetic composition according to 1 or 2, further comprising a humectant.
(4) The cosmetic composition according to any one of 1 to 3, further comprising an ultraviolet absorber.
(5) The cosmetic composition according to any one of 1 to 4, further comprising an antibacterial agent.
(6) The cosmetic composition according to 3, wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant.
(7) The group in which the UV absorber is composed of a benzoic acid UV absorber, an anthranilic acid UV absorber, a salicylic acid UV absorber, a cinnamic acid UV absorber, a triazine UV absorber, and a benzophenone UV absorber. 5. The cosmetic composition according to 4, wherein the cosmetic composition is at least one compound selected from the group consisting of:
(8) The cosmetic composition according to 5, wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent. (9) The cosmetic composition according to any one of 1 to 8, which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.

  By using a cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof and an anti-aging agent of the present invention, non-sticky cosmetics, quasi-drugs, medical products, hygiene products, and pharmaceuticals can be obtained. Can be offered. In particular, according to the present invention, it is possible to provide a skin external preparation excellent in a refreshing feeling without stickiness in a skin external preparation containing an anti-aging agent. In addition, poly-γ-L-glutamic acid and / or a salt thereof is a biologically-derived natural polymer and thus has excellent safety, and the cosmetic composition of the present invention can sufficiently withstand long-term use. Furthermore, since poly-γ-L-glutamic acid and / or a salt thereof can be produced from microorganisms, the raw material cost is low and mass production is possible.

  The cosmetic composition of the present invention contains poly-γ-L-glutamic acid and / or a salt thereof and an anti-aging agent as essential components.

  The “poly-γ-L-glutamic acid” of the present invention is a homopolymer consisting only of L-glutamic acid. Its structure is the structure represented by formula (I). The hydrogen of αCOOH may be hydrogen or another metal counter ion. For example, there is no need to limit the general materials such as sodium, potassium, magnesium, manganese, calcium, zinc and iron. Of these, sodium is preferred.

  The “molecular weight” of the present invention refers to the number average molecular weight (Mn) calculated in terms of the molecular weight of the pullulan standard substance.

  The poly-γ-L-glutamic acid of the present invention can be obtained by an existing method. For example, poly-γ-L-glutamic acid can be obtained by the method described in Patent Document 3 (Japanese Patent Application Laid-Open No. 2007-314434). Hereinafter, as an example, a method for producing poly-γ-L-glutamic acid with reference to Patent Document 3 will be described, but the present invention is not limited thereto.

  For example, the National Institute of Advanced Industrial Science and Technology (NIST) Patent Biological Depositary Center has Natrialba aegyptiaca 0830-82 shares (trusted organization: NPI Patent Biological Depositary Center), Date of Trust: Heisei April 4, 2006, accession number: FERM BP-20873), Natrialba aegyptiaca 0830-243 strain (trusted institution name: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary), accession date: 2006 April 4, 2000, accession number: FERM BP-20873), or Natrialba aegyptiaca 0831-264 strain (trusted institution: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary), accession date: 2006 Deposited as April 4, 2012, with accession number: FERM BP-20874) To obtain poly-gamma-L-glutamic acid using a with which strain is, it is possible to obtain poly-gamma-L-glutamic acid by liquid culture. Alternatively, a microorganism can be mutated by the method described in Patent Document 3 (Japanese Patent Application Laid-Open No. 2007-314434), a microorganism capable of producing poly-γ-L-glutamic acid by liquid culture is prepared, and poly-γ-L- Glutamic acid can also be produced. Moreover, it is also possible to produce poly-γ-L-glutamic acid by solid-phase culture of Natrialba aegyptiaca by a conventional method.

In the case of liquid culture, it is desirable to carry out under aerobic conditions such as shaking culture and aeration stirring culture. The culture temperature at that time is 30 to 50 ° C, preferably 35 to 45 ° C. The pH of the medium can be adjusted with sodium hydroxide, potassium hydroxide, ammonia, hydrochloric acid, sulfuric acid or an aqueous solution thereof, but is not limited as long as the pH can be adjusted. It is desirable to culture at a culture pH of 5.0 to 9.0, preferably at a pH of 6.0 to 8.5. In addition, the culture period is usually about 2 to 7 days. Further, it is desirable to culture at a NaCl concentration of 10 to 30%, preferably 15 to 25% during culture. Further, it is desirable to culture the yeast extract at a concentration of 0.1 to 10%, preferably 0.5 to 5.0%. In the case of solid culture, the culture temperature is 30 to 50 ° C., preferably 35 to 45 ° C., and the pH during the culture is 5.0 to 9.0, preferably pH 6.0, in the case of liquid culture and application. -8.5, NaCl concentration during culture is 10-30%, preferably 15-25%, Yeast Extract concentration is 0.1-10%, preferably 0.5-5%. When cultured in this manner, poly-γ-L-glutamic acid is mainly accumulated outside the cells and contained in the culture described above. Although not specifically limited, PGA production liquid medium-1 (22.5% NaCl, 2% MgSO ₄ .7H ₂ O, 0.2% KCl, 3% Trisodium Citrate, 1% Yeast Extract, 0.75% Casamino acid ) May be used, and the amount of each additive may be appropriately adjusted according to the strain.

  As a method for quantifying poly-γ-L-glutamic acid in a culture solution, precipitation is carried out from a sample containing poly-γ-L-glutamic acid using copper sulfate or ethanol, and the weight measurement of the precipitate and the Kijderder method (M. Bovarnick, J. Biol. Chem., 145, 415, 1942), a method for measuring the amount of glutamic acid after hydrochloric acid hydrolysis (RD Housewright, CB Thorne, J. Bacteriol., 60, 89, 1950) and a colorimetric method using quantitative binding with basic dyes (M. Bovarnick et al., J. Biol). Chem., 207, 593, 1954), preferably colorimetric using quantitative binding with basic dyes. It is.

  Examples of basic dyes include crystal violet, aniline blue, safranino, methylene blue, methyl violet, toluine nebula, congo red, azocarmine, thionine, and hematoxylin, with safranino being preferred.

  In order to separate and collect poly-γ-L-glutamic acid from this culture, the above-mentioned known methods such as precipitation using copper sulfate or ethanol may be used. As an example, for example, the culture solution is centrifuged to remove the cells. Subsequently, the obtained supernatant is diluted with 3 times the amount of water, and then the pH is adjusted to 3.0. After pH adjustment, the mixture was stirred at room temperature for 5 hours. Thereafter, 3 times the amount of ethanol was added, and poly-γ-L-glutamic acid was recovered as a precipitate. The precipitate is dissolved in 0.1 mM Tris-HCl buffer (pH 8.0), and low-molecular substances are removed by dialysis. After dialysis, the resulting solution may be treated with DNase or RNase for nucleic acid removal, and then with proteinase treatment for protein removal. After the proteinase treatment, low molecular substances may be removed by dialysis. After dialysis, dry poly-γ-L-glutamic acid may be obtained by freeze-drying or the like. Moreover, although refinement | purification using an anion exchange resin can be performed if necessary, it can refine | purify on general conditions.

The molecular weight of poly-γ-L-glutamic acid used in the present invention is not particularly limited, but is preferably 500,000 or more, more preferably 800,000 or more, still more preferably 1,000,000 or more, and particularly preferably 1.3 million or more.

The upper limit value of the molecular weight of L-PGA is not particularly limited, but according to the above-described L-PGA production method, for example, L-PGA having 6 million or 15 million at the maximum can be obtained.

  Since this poly-γ-L-glutamic acid and / or its salt is produced by archaea, it reduces the specific odor compared to poly-γ-L-glutamic acid and / or its salt produced by Bacillus natto Thus, the quality is not impaired even when used for cosmetics, quasi-drugs, medical supplies, hygiene products, or pharmaceutical applications.

  The amount of poly-γ-L-glutamic acid and / or salt thereof in the cosmetic composition may be added within a range that does not impair the action of poly-γ-L-glutamic acid and / or salt thereof. 001-50 mass% is preferable, 0.01-30 mass% is more preferable, 0.1-10 mass% is further more preferable, 0.5-5 mass% is especially preferable.

  The cosmetic composition of the present invention contains an anti-aging agent in addition to poly-γ-L-glutamic acid and / or a salt thereof. The anti-aging agent may be an anti-inflammatory agent and / or an antioxidant.

  Examples of anti-inflammatory agents include glycyrrhizic acid and salts thereof, glycyrrhetinic acid and salts thereof, isopropylaminocaproic acid and salts thereof, allantoin, lysozyme chloride, guaiazulene, methyl salicylate, and γ-oryzanol. Antioxidants include α-carotene. , Β-carotene, γ-carotene, lycopene, cryptoxanthine, lutein, zeaxanthin, isozeaxanthin, rhodoxanthine, capsanthin, crocetin, etc .; 1,4-diazacyclooctane, 2,5-dimethylfuran, 2-methylfuran, 2,5-diphenylfuran, 1,3-diphenylisobenzofuran, α-tocopherol, β-tocopherol, γ-tocopherol, d-tocopherol, histidine, tryptophan, methionine, alanine or its alkyl ester Tannins such as dibutylhydroxytoluene, butylhydroxyanisole, ascorbic acid, tannic acid, epicatechin, epicacatechin, epicatechin gallate, epicacatechin gallate, flavonoids such as rutin, other propyl gallates, astaxanthin, carotene, tocopherol , Ascorbic acid, tetrasodium edetate, erythorbic acid, tocopherol acetate, retinol acetate, dibitylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, methylsilanol dioletocopherol / silicic acid mixture, benzyl nicotinate, photosensitizer 401 , Aspartic acid, adenosine triphosphate 2Na, aminobutyric acid, fennel extract, Dutch mustard extract, caffeine, chlorella extract, safra Extract, ginger extract, soybean extract, tiso extract, folic acid, retinol, retinol palmitate, inositol, turmeric extract, oryzanol, carotene, carrot extract, wheat ointment extract, nematode extract, chimpi extract, touki extract, toki root extract, dodami extract, Tocopherol, tocopherol nicotinate, button extract, ergocalciferol, pyridoxine dicaprylate, batyl alcohol, glycyrrhetinyl stearate, glycyrrhizic acid, glycyrrhetinic acid, diphenhydramine hydrochloride, lysozyme, aminocaproic acid, litchi extract, yocuinine, merirot extract, button extract, Toki Extract, Toki Root Extract, Senkyu Extract, Genno Chocolate Extract, Allantoin, Arnica Extract Arnica flower extract, Ogon extract, Oren extract, Odorikosou extract, Catfish ear extract, Chamomile extract, Calamine, Kawaramugi extract, Licorice extract, Guaiazulene, Gardenia extract, Kumazasa extract, Glycyrrhizic acid 2K, Stearyl glycyrrhetic acid, Gentianana extract, Black tea extract, Comfrey Extract, Comfrey Leaf Extract, Tocopherol Acetate, Methyl Salicylate, Zinc Oxide, Shikon Extract, Murasaki Root Extract, Perilla Extract, Perilla Leaf Extract, Citrus Extract, Peonies Extract, Honeysuckle Extract, Sage Extract, Kizuta Extract, Elderflower Extract, Achillea millefolium extract, assembly extract, Sakuhakuhi extract, eucalyptus extract, pyridoxine HCl, loquat leaf extract, flyfish Scan, peach leaves, fruit extract, cornflower extract, saxifrage extract, mugwort extract, lettuce extract, Roman chamomile extract, burnet extract and Kakaroru, polyamines and the like.

  These anti-aging agents can be used individually by 1 type or in combination of 2 or more types.

Although the compounding quantity of the anti-aging agent used for this invention is not specifically limited, 15 weight% or less is good to the whole quantity of the composition in this invention, More preferably, 0.01 weight% or more and 10 weight% or less are good.

  The cosmetic composition of the present invention may contain a humectant in addition to poly-γ-L-glutamic acid and / or a salt thereof. The moisturizing agent may be a hydrophilic moisturizing agent and / or a lipophilic moisturizing agent. Examples of hydrophilic moisturizing agents include amino acids, peptides, proteins, higher alcohols and their derivatives, lipophilic moisturizing agents, phospholipids, glycolipids. Steroids may also be used.

  Examples of humectants include sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose, maltitol, sucrose, fructose, xylitol, sorbitol, maltotriose, threitol, erythritol, amylolytic sugar-reducing alcohol, sorbitol, ethylene glycol, 1 , 4-butylene glycol, diglycerin, triglycerin, tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanediol, other serine, glycine, threonine, alanine, collagen, Hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, Lyceroglycolipid, sphingophospholipid, glycosphingolipid, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, lychee mycelium culture or extraction thereof , Wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yakuinin extract, gallow extract, typhoid extract, diatomaceous earth extract, burdock extract, mannen wax extract, arnica extract, Wheat bran, pyrrolidone carboxylic acid, tomato extract, camellia oil, soybean phospholipid, hyaluronic acid, threonine, ammonium glycolate, methylsilanol alginate, yokuinin, toki extract, toki root extract, soybean extract, asparagus Gas extract, DNA-Na, PCA-Na, RNA-Na, ashitaba extract, aspartic acid, amachiya extract, alanine, arginine, sodium alginate, altea extract, aloe vera extract, oyster extract, barley leaf extract, oyster leaf, hydrolyzed keratin, Hydrolyzed collagen, hydrolyzed conchiolin, hydrolyzed eggshell membrane, hydrolyzed egg white, hydrolyzed silk, hydrolyzed soy protein, brown algae extract, karin extract, raspberry extract, xylitol, chitosan, cucumber extract, cucumber fruit extract, guava confection extract, Quince seed extract, glycine, glycerin, glucose, grapefruit extract, grapefruit fruit extract, clematis extract, burdock extract, rice fermentation broth, chondroitin sulfate Na, fish collagena, sun Shii extract, Shiitake extract, Giant extract, Glycerin, Cystine, Cysteine, Horsetail extract, Mallow extract, Serine, Sorbitol, Soy protein, Tomato extract, Lactic acid Na, Lactobacillus, Soybean fermented extract, Urea supine / Novara extract / Almond koji, Corn oil, Honey, hyaluronic acid Na, fukuno extract, betaine, loofah extract, maltitol, maltose, mannitol, lily extract, lactoferrin, lysine, apple extract, lotus extract, royal jelly, lanolin fatty acid polyethylene glycol 1000, lanolin fatty acid isopropyl, isopropyl myristate, myristic acid Isobutyl, hexyldecanol, myristyl lactate, lanolin fatty acid, tricaprylglyceryl, oleyl alcohol, oleic acid Chilled decyl, decyl oleate, reduced lanolin, octyl decanol, almond oil, avocado oil, olive oil, oleic acid, orange rough oil, cocoa butter, carrot extract, sesame oil, sasanqua oil, safflower oil, dihydrocholesterol, squalane, stearin Cholesteryl acid, ceramide 2, evening primrose oil, sunflower oil, sunflower oil, cefnide 3, sunflower seed oil, hybrid sunflower oil, phytosphingosine, grape seed oil, jojoba oil, jojoba seed oil, mineral oil, mink oil, macadamia nut oil, meadow Examples include foam oil, eucalyptus oil, eucalyptus leaf oil, lanolin, linoleic acid, rosehip oil, petrolatum and polyglutamic acid.

  These humectants can be used alone or in combination of two or more.

The blending amount of the humectant used in the present invention is not particularly limited, but is preferably 0.001% by weight or more and 50% by weight or less, preferably 0.002% by weight or more and 40% by weight based on the total amount of the composition in the present invention. % By weight or less is preferred, more preferably 0.005% by weight or more and 30% by weight or less.

  The cosmetic composition of the present invention may contain an ultraviolet absorber in addition to poly-γ-L-glutamic acid and / or a salt thereof. As UV absorbers, use benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, benzophenone UV absorbers, etc. Also good.

  Examples of the ultraviolet absorber include amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate, 2-ethoxyethyl paramethoxycinnamate, Octyl paramethoxycinnamate, glyceryl mono-2-ethylhexanoate diparamethoxycinnamate, isopropyl paramethoxycinnamate, diisopropyl diisopropylcinnamate ester mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxy Benzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydride Xylbenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4′-methoxy-dibenzoylmethane, 2,4,6-trianilino-p- (carbo-2′-ethylhexyl-1′-oxy) -1,3, 5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole, 2-ethylhexyl paradimethylaminobenzoate, ethyl paraaminobenzoate, topylmethoxydipenzoylmethane, oxybenzone-1, ethyl guaiazulenesulfonate, oxidation Examples thereof include zinc, sinoxate, and homomenthyl-N-acetylanthranilate.

  These ultraviolet absorbers can be used alone or in combination of two or more.

  The blending amount of the ultraviolet absorber used in the present invention is not particularly limited, but is preferably 0.01% by weight or more and 50% by weight or less, preferably 0.1% by weight or more, based on the total amount of the composition in the present invention. It is preferably 30% by weight or less, more preferably 0.5% by weight or more and 20% by weight or less.

  The cosmetic composition of the present invention may contain an antibacterial agent in addition to the poly-γ-L-glutamic acid and / or a salt thereof. The antibacterial agent may be an organic antibacterial agent and / or an inorganic antibacterial agent.

  Antibacterial agents include alum extract, halocarban, chlorophenesine, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexachlorophene, berberine, thioxolone, salicylic acid and their derivatives, benzoic acid, sodium benzoate , Paraoxybenzoic acid ester, parachlormetacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexachlorophene, chlorhexidine chloride, trichlorocarbanilide, photosensitizer, bis (2-pyridylthio) -1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, Bruno butoxyethanol, resorcinol, azulene, salicylic acid, zinc pyrithione, sodium mononitroguayacol, fennel extract, pepper extract, cetylpyridinium chloride, etc. benzethonium chloride and undecylenic acid derivatives.

  These antibacterial agents can be used alone or in combination of two or more.

  The blending amount of the antibacterial agent used in the present invention is not particularly limited, but is preferably 0.001% by weight or more and 10% by weight or less, and preferably 0.01% by weight or more and 8% or less, based on the total amount of the composition in the present invention. % By weight or less is preferable, more preferably 0.05% by weight or more and 5% by weight or less.

  The form of the cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof is not particularly limited. Therefore, it may take any state such as solid, liquid, paste, jelly, and powder. In order to form such a state, it can be solidified using, for example, a gelling agent, or can be dispersed using a liquid. Moreover, a solvent can be added to make a solution, or it can be spray-dried to form a powder.

  The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention has an excellent moisturizing effect, thickening action and hair improving action, and imparts appropriate moisture to the skin surface. It has been confirmed that it will remain firm and shiny.

  For example, a cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof of the present invention can be used as a cosmetic. Cosmetics include cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair dye, paste perfume, powder, pack, cleansing cream , Shaving cream, shaving lotion, tanning oil, sunscreen oil, tanning lotion, sunscreen lotion, tanning cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, beautiful nail enamel, beautiful nails Examples include enamel remover, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant, eau de cologne, hair nourishing agent, and hair restorer. The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention can also be used as an ointment or a poultice.

  The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention can be used by being blended with quasi-drugs, medical supplies, hygiene products, and pharmaceuticals in addition to cosmetics.

  When the cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention is implemented in the form of a quasi-drug, medical product, hygiene product, or pharmaceutical product, the dosage form is not limited, and an ampule , Capsules, powders, granules, pills, tablets, solids, solutions, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bath preparations, and the like.

  As quasi-drugs, medical supplies, hygiene products, and pharmaceuticals, for example, internal and external pharmaceutical preparations, skin lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, Hair cosmetics such as shampoo, rinse, hair treatment, hair cream, pomade, hair spray, hair conditioner, permanent agent, hair nick, hair dye, hair growth and hair restorer, foundation, white powder, funny, lipstick, blusher, eye shadow, Makeup cosmetics such as eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail care, perfumes, bath preparations, toothpastes, mouth fresheners, gargles, liquid odors and deodorants, Sanitary goods, sanitary cotton, wet tissue and the like. The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention can also be used as an ointment or a poultice.

  The cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof of the present invention is suitably applied to humans, but as long as each effect can be expected, animals other than humans It can also be applied to.

  The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention is a quasi-drug, medical product, hygiene product, pharmaceutical product as long as the effects of the present invention are not impaired as required. Ingredients and additives used in the above can be used in combination. These ingredients and additives include waxes, mineral oils, fatty acids, alcohols, esters, metal soaps, gums and water-soluble polymer compounds, surfactants, vitamins, amino acids, animals or plants, herbal medicines Extracts, extracts, microbial culture metabolites, α-hydroxy acids, inorganic pigments, astringents, bactericides / disinfectants, hair preparations, fragrances, pigments / colorants, sweeteners, nutrition enhancers and the like.

  For example, a cosmetic composition containing poly-γ-L-glutamic acid and / or a salt thereof, a nonionic surfactant, a lower alcohol, a polyhydric alcohol, an oil, a higher fatty acid, a higher alcohol, a synthetic ester, silicone, etc. May be used.

  For example, as the surfactant, anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant ( Carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester) Type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, and the like.

  Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate). Glycerol, penta-2-ethylhexyl diglycerol sorbitan, tetra-2-ethylhexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (eg mono cottonseed oil fatty acid glycerin, monoerucic acid glycerin, sesquioleate glycerin, glyceryl monostearate) , Α, α′-oleic acid pyroglutamate glycerin, monostearate glycerin malate, etc.); propylene glycol fatty acid esters (for example, monos Propylene glycol tea acrylate, etc.); hardened castor oil derivative; glycerin alkyl ether and the like.

  Examples of the POE-based hydrophilic nonionic surfactant include POE-sorbitan fatty acid esters (for example, POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate). POE sorbite fatty acid esters (eg, POE-sorbite monolaurate, POE-sorbite monooleate, POE-sorbite pentaoleate, POE-sorbite monostearate, etc.); POE-glycerin fatty acid esters (eg, POE-glycerol monostearate, POE-glycerin monoisostearate, POE-monooleate such as POE-glycerol triisostearate, etc.); POE-fatty acid esters (for example, POE-distearate) POE-alkyl ethers (for example, POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether), POE-monodiolate, ethylene glycol distearate, etc. POE-cholestanol ether, etc.); Pluronic type (for example, Pluronic, etc.); POE • POP-alkyl ethers (for example, POE • POP-cetyl ether, POE • POP-2-decyltetradecyl ether, POE • POP−) Monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether, etc.); tetra-POE / tetra-POP-ethylenediamine condensates (for example, Tetronic, etc.); POE-castor oil-cured castor Oil derivatives (eg POE-castor oil, POE-hardened castor oil, POE-hardened castor oil monoisostearate, POE-hardened castor oil triisostearate, POE-hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE -Hardened castor oil maleic acid, etc.); POE-beeswax lanolin derivatives (eg POE-sorbite beeswax etc.); alkanolamides (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide etc.); POE- Examples thereof include propylene glycol fatty acid ester; POE-alkylamine; POE-fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphate.

  Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol and the like.

Examples of the polyhydric alcohol include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, Pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, octylene glycol, etc.); trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6) Pentaerythritol such as hexanetriol, etc .; pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sorbitol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, dipropylene glycol, Tylene glycol, polypropylene glycol, tetraethylene glycol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol) Monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono 2-methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol Dibutyl Dihydric alcohol alkyl ethers (for example, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol) Monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol Dibutyl alcohol ether ester (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diazebate, ethylene glycol disuccine) Diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.); glycerin monoalkyl ether (example) Sugar alcohol (eg, sorbitol, maltitol, maltotriose, mannitol, sucrose, erythritol, glucose, fructose, amylolytic sugar, maltose, xylitol, starch decomposition) Sugar-reduced alcohol, etc.); glycolide; tetrahydrofurfuryl alcohol; POE-tetrahydrofurfuryl alcohol; POP-butyl ether; POP / POE-butyl ether; tripolyoxypropylene glycerin ether; POP-glycerin ether; POP · POE-pentane erythritol ether, polyglycerin and the like.
Examples of the higher alcohol include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathececinol, cholesterol, phytosterol, and isostearyl. Alcohol etc. are mentioned.
Examples of other alcohols include natural alcohols such as cholesterol and phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

  Oils include avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanca oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil , Beef tallow, sheep fat, lard, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane, etc. and its hardened oil, mineral oils such as liquid paraffin, petrolatum, synthesis of glycerin tripalmitate, etc. Triglycerin is mentioned.

    Examples of fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid and other natural fatty acids, isononanoic acid, caproic acid, 2- Synthetic fatty acids such as ethyl butanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid are exemplified. Examples of other higher fatty acids include linoleic acid, linolenic acid, isostearic acid, eicosapentaenoic acid, and docosahexaenoic acid.

For example, esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, lactic acid Myristyl, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate , Butyl stearate, dimethyloctanoic acid, and the like.
Examples of silicone include chain polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and three-dimensional network structures such as silicone resins.

  Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

  Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane, and pristane.

For example, examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylate.

  For example, gum and water-soluble polymer compounds include gum arabic, benzoin gum, danmar gum, guaiac oil, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, Carboxyalkylchitin, chitosan, hydroxyalkylchitin, low molecular chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose , Sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose Scan, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

  For example, as vitamins, retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (vitamin B1) ), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C, ascorbic acid and its derivatives, vitamin D In the group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group, phytonadione (vita) Emissions K1), menaquinone (vitamin K2), menadione (vitamin K3), and the like menadiol (vitamin K4).

  For example, amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, and the like, and sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

  For example, as an extract or extract of an animal or plant, herbal medicine, Asenyaku (Asen Yaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Achacha (Amacha), Aloe, Aloe Vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel (cage), turmeric (red gold), usba saishin (spicy), Japanese apricot (salmon plum), Japanese white beetle, walrus, green rose (fruit), hikikoshi (life-extending grass), Japanese oak (yellow cocoon), Koganebana (Ogon), Yamazakura ( Cherry bark), yellowfin, yellow orchid, ginseng, carrot, helicopter, licorice, holland garashi, orange, himehagi (distant), eel-brush (summer hay), tsurugokudami (what head-knot), enju (槐花）, mugwort (guy leaf), scallops (莪 朮), kudu (kazune), kanoko Cormorant (Valgarine), Chamomile, Kikarasuuri (Gurone), Kawara-momogi (Cervus chinensis), Licorice (licorice), Fukidan Popo (Cavity Winter Flower, Clover Winter Leaves), Raspberry, Kiwi Fruit, Japanese Clover (Kikkyo), Chrysanthemum (Chrysanthemum Flower) ), Catalpa (fruit), Citrus fruit (fruit), Tachibana (tachibana skin), cucumber, Udo or Shishido (living, solitary), apricot, wolfberry (bone skin, coconut, coconut leaf) , Clara, bittersweet, Kumazasa, grapefruit fruit, Nikkei (Kei-Kai), Keigai (Kai-Gai), Ebisugua (Keiko), Maruba morning glory or morning glory (Ken cow), safflower (Safflower), Gobaishi (Pyper), Confrey , Copaiba, gardenia, gentian, honoki (shoku), hinata taikozu (cow knee), goshuyu (burdock), burdock, butterfly Ngomushi (Gomiko), Rice, Rice bran, Wheat, Mishima Saiko (Shibahu), Saffron, Savonso, Hawthorn (Yamazako), Salamander (Samurai), Salvia, Sancynin carrot (Ginseng ginseng), Shiitake mushroom (Shiitake), Jiou (jihuang), Shikunshi (Mr. Kimiko), Murasaki (Shine), Perilla (Shiso leaf, Shiso), Oyster (柿蒂), Peonies (glaze), Psyllium (car forerunner, car front grass), Ginger (ginger), Shobu (Japanese cypress), Japanese hornworm (Sadako), Shimotsuke, Shiraba, Honeysuckle (Gin silver flower, Shinobu), Western ivy, Achillea millefolium, Elderberry, Redwood, Elderberry (Bone), Zeniaoi, Senkyu ), Assembly (this drug), mulberry (mulberry white skin, mulberry leaf), jujube (daegu), soybean, taranoki, chiketsu carrot (bamboo ginseng) ), Hanasuge (knowing mother), Waremokkou (earth), Dokudami (10 medicines), Fuyumushinatsusatake (Cycium Cordyceps), red pepper, physalis (Torone), Tachikakuso, Ryokucha (green tea), kocha (tea), clove (Clove), mandarin orange (Chen bark), camellia, camellia, capsicum (panju), crested sardine (Tochigi), red bellflower, daidai (orange peel), bituminous (ground), corn (southern fur), tochu (Tochu) , 杜 中葉), tomato, Nanten (Southern fruit), garlic (large sun), barley (malt), hakusen (white birch), janohi (barley winter), parsley, batata, mint (light load), hamamelis, rose , Loquat leaves, pine pods, grapes or their leaves, loofah, bodaiju, buttons (peony skin), hops, maikai , Matsuba, Maronie, Mannenrou, Mukuroji, Melissa, Merirot, Bokeh (Kiso), Palms, Peach (Momojin, Peach), Hiogi (Giraffe), Binroju (Mariko), Mejijiki (Menka), Yagurumaiku, Yukinoshita (Tiger ear) Grass), bayberry (Yellow Plum), Yashabushi (Yakuin), pearl barley (Yokuinin), mugwort, sagebrush, lavender, apple fruit, ganoderma biloba, lemon fruit, forsythia (rendense), forsythia, ginger , Hashiridokoro (roth root), chicken tosaka, cattle / human placenta extract, pig / cow stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, Elastin, elastin hydrolyzate, water-soluble elastin derivative, silk protein, sill Protein hydrolyzate, and the like cow blood cell protein hydrolyzate.

  Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder. .

  For example, examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

  For example, inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow oxide Examples include iron, bengara, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, and calamine.

  For example, astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid Etc.

  Examples of the bactericidal / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

  For example, selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thianthol, castari tincture, ginger tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate And thioglycolic acid.

  For example, as perfumes, natural animal perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, Iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil , Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, bell Vegetable fragrances such as Mott essential oil, Peruvian balsam essential oil, Boad Rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese seed mint essential oil, Other synthetic fragrances can be mentioned.

  For example, as a dye / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, an squid dye, a turmeric dye, an enju dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, a corn dye, Examples include onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment and rutin.

  Examples of sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.

  For example, examples of the nutrient fortifier include shell calcined calcium, cyanocolabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.

  Other ingredients and additives that can be incorporated into the cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof of the present invention include hormones, sequestering agents, pH adjusters, and chelating agents. , Antiseptic and antibacterial agents, fresheners, stabilizers, emulsifiers, animal / vegetable proteins and their degradation products, animal / plant polysaccharides and their degradation products, animal / plant glycoproteins and their degradation products, bloodstream Accelerators, anti-inflammatory agents / anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foaming agents, thickeners, oral preparations, deodorants / deodorants, bitters, seasonings, enzymes, etc. It is done.

  The cosmetic composition containing the poly-γ-L-glutamic acid and / or a salt thereof of the present invention can be widely used when a moisturizing effect, a thickening effect, a hair improving effect and the like are required. The amount to be used is determined within a range in which the desired moisturizing effect, thickening effect, hair improving effect and the like are sufficiently exhibited.

  Of course, the cosmetic composition of the present invention can be used as a cosmetic composition or a cosmetic because of the above-mentioned effects. In addition, the quasi-drug composition, the medical product composition, and the hygiene product composition are used. Products, pharmaceutical compositions, functional food compositions, quasi-drugs, medical supplies, hygiene products, pharmaceutical products, and premixed products.

  The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.

(Example 1) Poly-γ-L-glutamic acid production method (1) Production of poly-γ-L-glutamic acid
Natrialba aegyptiaca 0831-264 strain (accession number: obtained from Patent Document 2 (Japanese Patent Laid-Open No. 2007-314434)) and deposited at the National Institute of Advanced Industrial Science and Technology Patent Biological Deposit Center FERM BP-20874) single colony scraped with a platinum ear, 3 ml of PGA production liquid medium-1 (22.5% NaCl, 2.0% MgSO ₄ .7H ₂ O, 0.2% KCl, 3.0% Trisodium Citrate, 1.0% Yeast Extract, 0.75% Casamino acid) / 18 ml test tube x 5 were inoculated and cultured at 37 ° C. and 300 rpm for 3 days. 0.5 ml of the obtained culture broth was inoculated into 50 ml PGA production liquid medium-1 / 500 ml Sakaguchi flask × 10 and cultured at 37 ° C. for 5 days. The obtained culture broth was centrifuged to remove the cells. Subsequently, the obtained supernatant was diluted with 3 times the amount of water, and then the pH was adjusted to 3.0. After pH adjustment, the mixture was stirred at room temperature for 5 hours. Thereafter, 3 times the amount of ethanol was added and centrifuged, and poly-γ-L-glutamic acid was recovered as a precipitate. The precipitate was dissolved in 0.1 mM Tris-HCl buffer (pH 8.0) and dialyzed to remove low molecular weight material. After dialysis, the resulting solution was added to 1 mM MgCl ₂ , 10 U / ml DNase I (manufactured by TAKARA) and 20 μg / ml RNase I (manufactured by NIPPON GENE) and incubated at 37 ° C. for 2 hours. did. Subsequently, 3 U / ml Proteinase K (manufactured by TAKARA) treatment was incubated at 37 ° C. for 5 hours for protein removal. After proteinase K treatment, dialyzed against MilliQ water to remove low molecular weight substances. After dialysis, poly-γ-L-glutamic acid was adsorbed on an anion exchange resin, Q sepharose Fast Flow (manufactured by Amersham Biosciences), washed and eluted with 1M NaCl. The resulting solution was dialyzed against MilliQ water, and the solution after dialysis was freeze-dried to obtain poly-γ-L-glutamic acid / Na salt.
The H-NMR spectrum (500 MHz) of the obtained poly-γ-L-glutamic acid was measured using heavy water, and confirmed to be poly-γ-L-glutamic acid.

(2) GPC analysis and IR analysis of Na salt The average molecular weight of the obtained poly-γ-L-glutamic acid / Na salt was measured by GPC analysis. IR analysis was also performed.
As a result of GPC analysis, it was confirmed that Mw = 7,522,000, Mn = 3,704,000, Mw / Mn = 2.031 (in pullulan conversion).
The GPC analysis conditions are as follows.
Apparatus: HLC-8220GPC (manufactured by Tosoh Corporation)
Column: TSKgel α-M (manufactured by Tosoh Corporation)
Flow rate: 0.6ml / min
Eluent: 0.15M NaCl aqueous solution Column temperature: 40 ° C
Injection volume: 10 μl
Detector: Differential refractometer
IR analysis showed that it was a Na salt.

(3) Production of free form and GPC analysis / IR analysis In the poly-γ-L-glutamic acid purification step, it was adsorbed on an anion exchange resin, Q sepharose Fast Flow (manufactured by Amersham Biosciences), washed and eluted with 1M NaCl. The pH of the poly-γ-L-glutamic acid-containing solution was adjusted to pH 2.0 with 1N HCl. Then, it dialyzed with MilliQ water and further freeze-dried to obtain poly-γ-L-glutamic acid-free form. The average molecular weight of the resulting free form of poly-γ-L-glutamic acid was measured by GPC analysis. IR analysis was also performed.
As a result of GPC analysis, it was confirmed that Mw = 2,888,000, Mn = 1,327,000, Mw / Mn = 2.176 (in pullulan conversion).
The GPC analysis conditions are as follows.
Apparatus: HLC-8220GPC (manufactured by Tosoh Corporation)
Column: TSKgel α-M (manufactured by Tosoh Corporation)
Flow rate: 0.6ml / min
Eluent: 0.5M NaCl aqueous solution Column temperature: 40 ° C
Injection volume: 10 μl
Detector: Differential refractometer
The result of IR analysis showed that it was free.

(Example 2) Production of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent A cosmetic liquid having the composition shown below was produced by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 1), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 3) Manufacture of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent and moisturizer A cosmetic liquid having the composition shown below was manufactured by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 2), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Moisturizer: Hyaluronic acid 0.01
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 4) Production of a poly-γ-L-glutamic acid cosmetic liquid containing an anti-aging agent and an ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 3), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 5) Manufacture of poly-γ-L-glutamic acid serum containing anti-aging agent and antibacterial agent A serum having the composition shown below was manufactured by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 4), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 6) Production of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent, humectant and ultraviolet absorber A cosmetic liquid having the composition shown below was produced by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 5), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Moisturizer: Hyaluronic acid 0.01
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 7) Production of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent, moisturizing agent and antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 6), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Moisturizer: Hyaluronic acid 0.01
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 8) Production of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent, ultraviolet absorber and antibacterial agent A cosmetic liquid having the composition shown below was produced by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 7), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

(Example 9) Manufacture of poly-γ-L-glutamic acid cosmetic liquid containing anti-aging agent, moisturizer, ultraviolet absorber and antibacterial agent A cosmetic liquid having the composition shown below was manufactured by a conventional method. As poly-γ-L-glutamic acid, poly-γ-L-glutamic acid / Na salt described in Example 1 was used. As a control (Comparative Example 8), a serum containing no poly-γ-L-glutamic acid was also produced by a conventional method.
(Composition) (wt%)
Oil phase component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
Poly-γ-L-glutamic acid 2.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-aging agent: CoQ10 0.05
Moisturizer: Hyaluronic acid 0.01
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Evaluation of serum (control zone)
Using the cosmetic liquid of the comparative example, five panelists evaluated the spread, lack of tingling, moist feeling, and difficulty in sticking when using this, and judged according to the following criteria.
The results are shown in Table 1.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

Evaluation of serum (poly-γ-L-glutamic acid)
Using the serum of the composition shown in Example 8, five panelists evaluated the spread, no tingling, moist feeling, and difficulty in sticking when used, and determined according to the following criteria: did. The results are shown in Table 2. In both cases, the inclusion of poly-γ-L-glutamic acid has a good feeling of spreading and moistness, and the tingling sensation as an index of irritation is eliminated. The item was evaluated as good.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

  The cosmetic composition containing the poly-γ-L-glutamic acid and / or salt thereof and the anti-aging agent of the present invention is non-sticky and has a refreshing feeling. Cosmetics, quasi-drugs, medical products, hygiene products, pharmaceuticals Can be provided. Furthermore, since the raw material cost is low, mass production is possible, and it can sufficiently withstand long-term use, it is expected to contribute greatly to the industry.

Claims (9)

  A cosmetic composition comprising poly-γ-L-glutamic acid and / or a salt thereof, and an anti-aging agent.   The cosmetic composition according to claim 1, wherein the anti-aging agent is an anti-inflammatory agent and / or an antioxidant.   The cosmetic composition according to claim 1, further comprising a humectant.   Furthermore, an ultraviolet absorber is contained, The cosmetic composition in any one of Claims 1-3 characterized by the above-mentioned.   Furthermore, an antibacterial agent is contained, Cosmetic composition in any one of Claims 1-4 characterized by the above-mentioned.   The cosmetic composition according to claim 3, wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant.   The UV absorber is selected from the group consisting of benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, and benzophenone UV absorbers. The cosmetic composition according to claim 4, wherein the cosmetic composition is at least one compound.   6. The cosmetic composition according to claim 5, wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent.   The cosmetic composition according to any one of claims 1 to 8, wherein the cosmetic composition is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.