JP2009149567A - Cosmetic composition containing biosurfactant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic composition which contains a biosurfactant having a moisture-retaining action, a collagen production-promoting action, a cell-activating action, an anti-aging action, a hair-growing action and a chapped skin-improving action, in other words, to provide a moisture-retaining agent, a collagen production-promoting agent, a cell activating agent, an anti-aging agent, a hair-growing agent and a chapped skin-improving agent which each contains the cosmetic composition containing the biosurfactant, to provide a cosmetic composition, a quasi drug composition, a medical supply composition, a sanitary good composition, and a medicine composition which each contains the cosmetic composition, and to provide a cosmetic, a quasi drug, a medical supply, a sanitary good and a medicine which each contains the composition. <P>SOLUTION: This cosmetic composition contains at least a biosurfactant and a moisture-retaining agent. The cosmetic composition preferably further contains a UV ray-absorbing agent, and an antibacterial agent. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a cosmetic composition containing a biosurfactant as an active ingredient, and the cosmetic composition acts as a moisturizer, collagen production promoter, cell activator, anti-aging agent, ultraviolet absorber and rough skin improving agent. In particular, the present invention relates to cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals containing biosurfactants that are effective in preventing aging and preventing hair growth and hair loss. More specifically, the biosurfactant includes mannosyl erythritol lipid (MEL) or mannosyl alditol lipid (MAL) containing an alditol other than erythritol (MAL includes mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid ( MAraL) and mannosyl ribitol lipid (MRL)).

  Individual aging and the various diseases that accompany this are closely related to the aging of all dividing cells (decrease in division rate and cell function). For example, the skin is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that support these extracellular skin structures. In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin has a firm and glossy appearance and is kept fresh. However, aging, ultraviolet rays, drying, stress, etc. cause deterioration of the function of the extracellular matrix and fibroblasts in particular. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its tension and gloss and becomes rough. Aging symptoms such as wrinkles and dullness occur.

  Searching for an activator and an anti-aging agent has been carried out for the purpose of preventing or suppressing aging at the cellular level. For example, connective tissue hydrolysates (Patent Document 1), thymus / spleen-derived water-soluble protein (Patent Document 2), bovine placenta extract (Patent Document 3) and the like are known as animal-derived activators. Examples of plant-derived activators include sesame seeds, sayaks, capsicum, toki, dokudami, bakumondo (patent document 4), almonds, dandelion, elderberry, cucumber, assembly, perch, carrots, carrots, hops, mokuge, and yokoinin (patents) Document 5, Patent Document 6), extracts of Ginger family Turmeric (Patent Document 7), Hanayoriaceae Hanayasuri (Patent Document 8) and the like are known. Some of these are used as activating agents and anti-aging agents in quasi-drugs and cosmetics, but no activating agents or anti-aging agents that exhibit satisfactory effects have been obtained.

  In addition, studies have been made to prevent or cure rough skin. Rough skin refers to dry skin in which exfoliation of keratinocytes is generally observed. Such rough skin is caused by elution of interstitial lipids such as cholesterol, ceramide, fatty acids, etc., and formation of a stratum corneum permeation barrier due to degeneration of keratinocytes caused by ultraviolet rays, detergents, etc., and disruption of proliferation / keratinization balance of epidermal cells. Etc. For the purpose of preventing or healing this rough skin, studies have been made on supplying a keratinocyte lipid component or a synthetic keratinocyte lipid similar thereto.

  This stratum corneum intercellular lipid is a lamellar granule that is biosynthesized by cells in the spinous layer and the granule layer, and is released between the cells just below the stratum corneum and extends, taking a lamellar (lamellar) structure. It has spread to. The lamellar granule is composed of glucosylceramide, cholesterol, ceramide, phospholipid, etc., but the horny layer intercellular lipid hardly contains glucosylceramide. That is, glucosylceramide in lamellar granules is hydrolyzed by β-glucocerebrosidase and converted to ceramide. As a result of this ceramide having a lamellar structure, formation of a stratum corneum permeation barrier is improved as a stratum corneum intercellular lipid. Therefore, it is considered to have a barrier function for preventing rough skin. It has been reported that ceramide supplementation is effective for rough skin caused by a cleaning agent, and shows a high effect in improving rough skin (Non-patent Document 1).

  However, a plant extract containing glucosylceramide as a main component has not yet obtained satisfactory results as an alternative to ceramide, and the synthesis of ceramide is costly to obtain a large number of reaction steps.

  Mannosyl erythritol lipid (MEL) is a natural surfactant produced by yeast, and various physiological actions have been reported (Non-patent Document 1). Recently, mannosyl mannitol lipid (MML) in which erythritol replaces mannitol has been found (Patent Document 9). Uses as external preparations and cosmetics are useful as anti-inflammatory agents and antiallergic agents (Patent Document 10), hair nourishing and hair-restoring agents (Patent Document 11), antibacterial action (Patent Document 12), and surface tension reduction. The action (Patent Document 13) is known. Moreover, the effect (patent document 14) which MEL acts as a substitute of ceramide and improves rough skin has also been reported.

However, it has not been reported that the moisturizing effect is improved by coexisting MEL with a humectant.
JP-A-62-84024 Japanese Patent Laid-Open No. 63-188867 Japanese Patent Laid-Open No. 03-141299 Japanese Patent Laid-Open No. 10-45615 Japanese Patent Laid-Open No. 10-036279 Japanese Patent Laid-Open No. 10-36279 JP 2004-75632 A JP 2005-89375 A JP 2005-104837 A JP 2005-68015 A JP 2003-261424 A JP-A-57-145896 JP-A-61-205450 WO2007 / 060956 Journal of Bioscience and Bioengineering, 94,187 (2002)

  Giving activation and anti-aging to mammals, especially humans, is an extremely important issue, and various animal-derived and plant-derived activators and anti-aging agents have been found, but they can actually be used industrially. A sufficient and stable effect has not been obtained to date, and new activators and anti-aging agents are currently being searched. Ceramide is used in cosmetics as a useful component for rough skin, but synthetic products or plant extracts are expensive and can be used only in small amounts.

  It is an object of the present invention to provide a cosmetic composition containing a biosurfactant having improved moisturizing effect, collagen production promoting effect, cell activating effect, anti-aging effect, hair-restoring effect, rough skin improving effect, etc., which has improved the above problems. . In other words, the object is to provide a moisturizer, a collagen production promoter, a cell activator, an anti-aging agent, a hair-restoring agent, a rough skin improving agent and the like including a cosmetic composition containing a biosurfactant. Further, a cosmetic composition containing the cosmetic composition, a quasi-drug composition, a medical product composition, a hygiene product composition, a pharmaceutical composition, and a cosmetic, quasi-drug, medical product, hygiene containing the composition. It is to provide supplies and medicines.

  As a result of diligent efforts to achieve the above object, the present inventors have made MAL containing alditols other than biosurfactants, particularly MEL and erythritol (MAL includes mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabi By including a cosmetic composition containing tall lipid (MAraL), mannosyl ribitol lipid (MRL), triacyl MEL and triacyl MAL together with a moisturizer, the skin permeability of the moisturizer is improved and its effect is enhanced. At the same time, the inventors have found that the characteristic that the stimulation can be reduced can be exhibited, and have completed the invention. That is, this invention relates to the technique shown below.

1. A cosmetic composition comprising at least a biosurfactant and a humectant.
2. 2. The cosmetic composition according to 1, wherein the biosurfactant has a mannose skeleton.
3. 3. The cosmetic composition according to 1 or 2, wherein the biosurfactant is a glycoside bond of a sugar alcohol to the hydroxyl group at the 1-position of the mannose skeleton.
4). The biosurfactant having a mannose skeleton is mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and / or mannosyl ribitol lipid (MRL) 3. The cosmetic composition according to 3.
5. MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), a triacyl derivative of MEL-A, 5. The cosmetic composition according to 4, wherein the cosmetic composition is at least one compound selected from the group consisting of a triacyl form of MEL-B, a triacyl form of MEL-C, and a triacyl form of MEL-D.
6). The cosmetic composition according to any one of 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid.
7). The cosmetic composition according to any one of 1 to 6, wherein the biosurfactant forms a lamellar structure and / or a liposome.
8). 8. The cosmetic composition according to 7, wherein a coexisting active ingredient is embedded in a lamellar structure formed by a biosurfactant and / or a liposome.
9. The cosmetic composition according to any one of 1 to 10, wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant.
10. Furthermore, the cosmetic composition in any one of 1-9 characterized by containing a ultraviolet absorber.
11. Furthermore, an antibacterial agent is contained, Cosmetic composition in any one of 1-10 characterized by the above-mentioned.
12 The UV absorber is selected from the group consisting of benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, and benzophenone UV absorbers. 11. The cosmetic composition according to 10, wherein the cosmetic composition is one or more compounds.
13. 12. The cosmetic composition according to 11, wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent.
14 The cosmetic composition according to any one of 1 to 13, which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.

The cosmetic composition containing the biosurfactant of the present invention has effects such as moisturizing action, collagen production promoting action, cell activation action, anti-aging action, hair growth action and rough skin improving action, etc., cosmetics, quasi drugs, medical supplies Can provide hygiene products, medicines.
In addition, since biosurfactant is a biologically derived natural surfactant, it is excellent in safety, and the cosmetic composition according to the present invention has an effect that it can sufficiently withstand long-term use. Furthermore, since biosurfactants can be produced by culturing microorganisms, raw material costs are low, and mass production is possible. Therefore, the cosmetic composition according to the present invention has an effect that it can be provided at a low price. The biosurfactant may be used as a premix product.

  A cosmetic composition containing a biosurfactant has both cleansing properties, moisturizing effect, collagen production promoting effect, cell activation effect, anti-aging effect, hair growth effect and rough skin improving effect, etc. Useful.

(Activator)
In this specification, “activation” is intended to maintain or enhance cell function or cell activity. As a result, a decrease in cell function or cell activity can be suppressed, that is, cell aging can be suppressed. Therefore, “activator” is synonymous with “cell activator” and has utility as “anti-aging agent”.

  For example, “activation, anti-aging” in skin cells means skin wrinkles, sagging, hardening, etc. by reducing the decrease in skin cell function associated with the accumulation of structural changes in the underlying membrane due to aging and photoaging. This refers to maintaining a youthful, healthy skin condition with prevention and improvement. In addition, for example, in dermal papilla cells or hair matrix cells, it is possible to suppress hair loss by maintaining the hair cycle by reducing the functional degradation of dermal papilla cells or hair matrix cells due to aging, stress, hormone balance, etc. pointing.

(Biosurfactant)
“Biosurfactant” is a general term for substances having surface-active ability and emulsifying ability produced by living organisms, and not only exhibits excellent surface activity and high biodegradability, but also has various physiological functions. Therefore, there is a possibility of developing different behaviors and functions from synthetic surfactants. Biosurfactants can be produced in large quantities by culturing microorganisms and may be used as premixed products.

  A “premix product” is a product obtained by adding a dispersing agent or the like in addition to a functional material, or diluting with a solvent so that it can be easily used during cosmetic production.

  Biosurfactants include mannosyl erythritol lipid (MEL), mannosyl alditol lipid (MAL) (for MAL, mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), mannosyl ribitol lipid (MAL). MRL)), trehalose lipids, rhamnolipids, sophorolipids, surfactins, spiculsporic acids, emalzanes, etc., and any of them can be used as the activator according to the present invention. Among these, it is preferable to use a biosurfactant that forms a lamellar structure, and MEL and MAL are particularly preferable.

(MEL)
Four types of MELs are known, MEL-A, MEL-B, MEL-C and MEL-D, depending on the presence or absence of acetyl groups at the 4th and 6th positions of mannose. Formula (I) shows the structure of MEL-A. In formula (I), R1 and R2 represent a hydrocarbon group. That is, MEL-A is a compound having an alkanoyl group having 5 to 19 carbon atoms at the 2nd and 3rd positions of mannose and the acetyl group at the 4th and 6th positions of mannose in the formula (I). In MEL-B, mannose 4-position acetyl group (CH ₃ CO) is H in formula (I), and MEL-C is mannose 6-position acetyl group (CH ₃ CO) in formula (I). H and MEL-D is H in the acetyl group (CH ₃ CO) at positions 4 and 6 of mannose in formula (I).

[R ¹ and R ² are the same or different and each represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, preferably 1 to 16 carbon atoms, more preferably a straight chain or branched chain having 1 to 14 carbon atoms, or An alkenyl group, alkadienyl group or alkatrienyl group having a straight chain or branched chain having 2 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 2 to 14 carbon atoms. n represents an integer of 6 to 10, and m represents an integer of 6 to 10, respectively. ]

According to the formula (I), MEL is a saturated or unsaturated straight chain having 1 to 19, preferably 1 to 17, more preferably 1 to 15 carbon atoms at the 2nd and 3rd positions of mannose. Alternatively, it is a compound having a branched alkanoyl group and the mannose 4th and 6th positions are acetyl groups (one of the mannose 4th and 6th acetyl groups, or both are hydroxyl groups). May be good). The total number of carbon atoms of the 2nd and 3rd alkanoyl groups is preferably 10 to 38, more preferably 14 to 30.

Preferable groups for R ¹ and R ² include C _{7 to} C ₁₁ alkyl groups such as CH ₃ (CH ₂ ) ₆ , CH ₃ (CH ₂ ) ₈ , and CH ₃ (CH ₂ ) ₁₀ . MEL-A of the formula (I) or (IA) in which R ¹ and R ² are C _{7 to} C ₁₁ alkyl groups is myristic acid, palmitic acid, stearic acid, oleic acid or esters thereof (monoalkyl esters, mono -, Di-, tri-glycerides, or fats and oils containing these saturated or monounsaturated fatty acids) can be obtained by adding them to the medium. Similarly, MEL-A of the formula (I) in which R ¹ and R ² are alkenyl group, alkadienyl group or alkatrienyl group is linoleic acid, linolenic acid, arachidonic acid having two or more double bonds as raw materials. It can be obtained by adding raw materials such as esters (monoalkyl esters, mono-, di-, tri-glycerides, oils and fats containing these highly unsaturated fatty acids, etc.) to the medium. Preferred alkanoyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkenyl groups such as C ₇ H ₁₄ , C ₉ H ₁₈ , and C ₁₁ H ₂₂ . Preferable alkadienyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkadienyl groups such as C ₇ H ₁₂ , C ₉ H ₁₆ , and C ₁₁ H ₂₀ . Preferable alkatrienyl groups represented by R ¹ and R ² include C _{7 to} C ₁₁ alkatrienyl groups such as C ₇ H ₁₀ , C ₉ H ₁₄ , and C ₁₁ H ₁₈ .

(MML)
The structure of MML is shown in the formula (II), and the preferred MML is shown in the formula (IIA) (wherein either one of the acetyl groups at the 4-position and 6-position of mannose, or both may be a hydroxyl group). ). According to the formula (II), MML is a saturated or unsaturated straight chain having 1 to 19, preferably 1 to 17, more preferably 1 to 15 carbon atoms at the 2nd and 3rd positions of mannose. Alternatively, it is a compound having a branched alkanoyl group and the mannose 4th and 6th positions are acetyl groups (wherein either one of the mannose 4th and 6th acetyl groups, or both are hydroxyl groups) Note that the total number of carbon atoms in the 2nd and 3rd alkanoyl groups is preferably 10 to 38, more preferably 14 to 30.

[R ¹ and R ² are the same or different and each represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, preferably 1 to 16 carbon atoms, more preferably a straight chain or branched chain having 1 to 14 carbon atoms, or An alkenyl group, alkadienyl group or alkatrienyl group having a straight chain or branched chain having 2 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 2 to 14 carbon atoms. n represents an integer of 6 to 10, and m represents an integer of 6 to 10, respectively. ]

(MAL)
The structure of MAL is shown in the formula (III) (wherein either one of the acetyl groups at the 4th and 6th positions of mannose, or both may be a hydroxyl group). According to the formula (III), MAL is a saturated or unsaturated linear chain having 1 to 19, preferably 1 to 17, more preferably 1 to 15 carbon atoms at the 2nd and 3rd positions of mannose. Alternatively, it is a compound having a branched alkanoyl group and the mannose 4th and 6th positions are acetyl groups (wherein either one of the mannose 4th and 6th acetyl groups, or both are hydroxyl groups) Note that the total number of carbon atoms in the 2nd and 3rd alkanoyl groups is preferably 10 to 38, more preferably 14 to 30.

[R ¹ and R ² are the same or different and each represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, preferably 1 to 16 carbon atoms, more preferably a straight chain or branched chain having 1 to 14 carbon atoms, or An alkenyl group, alkadienyl group or alkatrienyl group having a straight chain or branched chain having 2 to 18 carbon atoms, preferably 2 to 16 carbon atoms, more preferably 2 to 14 carbon atoms. n represents an integer of 6 to 10, and m represents an integer of 6 to 10, respectively. ]

(Triacyl derivative)
The biosurfactant used in the activator according to the present invention may be a MEL triacyl or MML triacyl. The triacyl biosurfactant is a biosurfactant having a novel structure having higher hydrophobicity than MEL and MML. For example, when a large amount is obtained from other than the culture solution of MEL-producing bacteria, it can be produced by reacting MEL with various vegetable oils using enzymes.

  The triacyl form of MEL, that is, triacyl mannosyl erythritol lipid (sometimes referred to as triacyl MEL) has a structure of the formula (IV).

  In formula (IV), R 1, R 2 and R 3 each independently represent a hydrocarbon group or an oxygen atom-containing hydrocarbon group, and one or both of the hydroxyl groups at the 4-position and 6-position of mannose are acetylated. It may be substituted with a group. The hydrocarbon group may have only a saturated bond or may have an unsaturated bond. When it has an unsaturated bond, it may have a plurality of double bonds. The carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not limited.

  In the formula (IV), R1 and R2 preferably have 6 to 20 carbon atoms, and have an ester bond with the hydroxyl groups at the 2nd and 3rd positions of mannose as the aliphatic acyl group (RCO-), An acetyl group may be ester-bonded to the hydroxyl group. R3 preferably has 6 to 20 carbon atoms and has an ester bond with a primary hydroxyl group of erythritol as an aliphatic acyl group (RCO-).

  Triacyl MEL has a structure in which a fatty acid ester is added and has high hydrophobicity, and therefore is superior as an emollient in that it can be easily combined with various oil components as compared with conventional MEL.

  These biosurfactants may be used alone, or two or more biosurfactants can be used in combination.

(Biosurfactant production method)
The method for producing the biosurfactant is not particularly limited, and a fermentation method using microorganisms may be arbitrarily selected. For example, culture production of MEL (MEL-A, MEL-B, MEL-C) can be produced by Pseudozyma antarctica NBRC 10736 according to a conventional method, and Pseudozyma antarctica, Pseudozyma sp., Etc. can be used as microorganisms. . It is a well-known fact that an MEL mixture can be easily obtained with any microorganism. The MEL mixture can be purified using silica gel column chromatography to isolate MEL-A, MEL-B and MEL-C. Moreover, as bacteria which produce MEL-B, Pseudozyma antarctica and Pseudozyma tsukubaensis are known, and these bacteria may be used. Pseudozyma hubeiensis is known as a bacterium that produces MEL-C, and the bacterium may be used. The microorganism having the ability to produce MEL is not particularly limited, and can be appropriately used depending on the purpose.

  Fermentation media for producing biosurfactants are generally composed of N sources such as yeast extract and peptone, C sources such as glucose and fructose, and inorganic salts such as sodium nitrate, dipotassium hydrogen phosphate and magnesium sulfate heptahydrate. A medium with a typical composition can be used, including oils and fats such as olive oil, soybean oil, sunflower oil, corn oil, canola oil and coconut oil, and water-insoluble substrates such as hydrocarbons such as liquid paraffin and tetradecane. These can be used alone or in combination of two or more.

  Fermentation conditions such as pH and temperature, culture time, and the like can be arbitrarily set, and the culture solution after fermentation can be used as it is as the biosurfactant of the present invention. In addition, it is possible to appropriately add any operation such as filtration, centrifugation, extraction, purification, sterilization and the like to the culture solution after fermentation, and the obtained extract can be diluted, concentrated and dried. .

  As oils and fats used as raw materials, vegetable oils and fats are preferable. Vegetable fats and oils are not particularly limited, and can be appropriately selected according to the purpose. For example, soybean oil, rapeseed oil, corn oil, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, palm oil, etc. Among them, soybean oil and olive oil are biosurfactants (especially MEL) production efficiency (production amount) , Production rate, and yield) are particularly preferable. These may be used alone or in combination of two or more.

  There is no restriction | limiting in particular as an inorganic nitrogen source, Although it can select suitably according to the objective, For example, ammonium nitrate, urea, sodium nitrate, ammonium chloride, ammonium sulfate, etc. are mentioned.

  There are no particular limitations on the method for recovering and purifying the biosurfactant, and it can be appropriately selected according to the purpose. For example, it can be recovered by centrifuging the culture solution to recover the oil, and extracting and concentrating with an organic solvent such as ethyl acetate.

Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, diethyl ether, and dioxane. , Esters such as acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in any combination of two or more kinds, and each solvent extract is combined. Can also be used.

  There are no particular limitations on the extraction method, but usually it may be in the range of the boiling point of the solvent from room temperature to normal pressure. After extraction, it is filtered or adsorbed, decolored and purified using an ion exchange resin to form a solution. , Paste, gel, and powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. As a purification treatment means such as deodorization and decolorization, an activated carbon column or the like may be used, and a normal means generally applied depending on the extracted substance may be arbitrarily selected. If necessary, a high-purity biosurfactant can be obtained by purification using a silica gel column.

(Triacyl production method)
A method for obtaining a biosurfactant triacyl form will be described by taking a method for producing a MEL triacyl form as an example, but the biosurfactant triacyl form used in the present invention is not limited to a triacyl MEL.

  For example, in order to obtain a triacyl MEL, a triacyl MEL fraction can be purified from a culture solution produced by fermenting a microorganism as described above. In order to obtain a large amount, MEL is dissolved in an organic solvent, a fatty acid derivative such as vegetable oil is added, and esterification or transesterification is performed in the presence of a hydrolase.

  The fatty acid introduced into the erythritol part of MEL may be a long-chain hydrocarbon monovalent carboxylic acid. Further, it may be a saturated fatty acid or an unsaturated fatty acid. In the case of an unsaturated fatty acid, it may have a plurality of double bonds. The carbon chain may be linear or branched. Furthermore, a fatty acid derivative that is a derivative of a fatty acid may be used in the present invention, or a mixture of a fatty acid and a fatty acid derivative may be used in the present invention. The fatty acid or fatty acid derivative introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids, and synthetic esters.

  The “oils” may be vegetable oils, animal oils, mineral oils, and hardened oils thereof. Specifically, avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanqua oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil Animal and vegetable oils such as beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane and their hardened oils. Examples thereof include mineral oil such as liquid paraffin and petrolatum, and synthetic triglycerin such as glycerin tripalmitate. Preferably avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamia nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, southern oil, castor oil, flaxseed oil , Safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, and more preferably olive oil and soybean oil.

  Examples of the “higher fatty acid” include caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, Examples include undecic acid, tolic acid, eicosapentaenoic acid, docosahexaenoic acid, and the like. Preferred are lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid and undecylenic acid, and more preferred are oleic acid, linoleic acid and undecylenic acid.

  Examples of the “synthetic ester” include methyl caproate, methyl caprylate, methyl caprate, methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, undecine. Methyl acid, ethyl caproate, ethyl caprylate, ethyl caprate, ethyl laurate, ethyl myristate, ethyl palmitate, ethyl oleate, ethyl linoleate, ethyl linolenate, ethyl stearate, ethyl undecinate, vinyl caproate , Vinyl caprylate, vinyl caprate, vinyl laurate, vinyl myristate, vinyl palmitate, vinyl oleate, vinyl linoleate, vinyl linolenate, vinyl stearate, vinyl undecinate, cetyl octanoate , Octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, Oren'i acid decyl dimethyl octanoate, cetyl lactate, myristyl lactate, and the like. Preferred are methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, methyl undecylate, more preferably methyl oleate, methyl linoleate, and methyl undecylate. .

  Triacyl MEL can be produced by dissolving MEL in an organic solvent and reacting as described above. The organic solvent is not limited as long as it can solubilize MEL. What is necessary is just to be able to solubilize a part even if not all can be solubilized. The organic solvent may be a mixture of a plurality of organic solvents. Specifically, for example, methanol, ethanol, propanol, butanol, acetone, propanone, butanone, pentan-2-one, 1,2-ethanediol, 2,3-butanediol, dioxane, acetonitrile, 2-methyl-butane -2-ol, tertiary butanol, 2-methylpropanol, 4-hydroxy-2-methylpentanone, tetrahydrofuran, hexane, DMF, DMSO, pyridine, methyl ethyl ketone and the like. Acetone, tetrahydrofuran, tertiary butanol, acetonitrile and dioxane are preferable, and acetone is more preferable.

  Examples of hydrolases include lipases, proteases, and esterases. It is preferable to use at least one selected from these, and a plurality of hydrolases may be used. Lipase and esterase are preferable, and lipase is more preferable.

  Specifically, for example, MEL purified from a culture solution of MEL-producing microorganisms is dissolved in an organic solvent (for example, acetone), and commercially available lipase (for example, Novozyme 435 (manufactured by Novozymes) etc.) and vegetable oils and fats are dissolved therein. Added.

  In the case of this production method, the reaction temperature is 10 to 100 ° C., preferably 20 to 50 ° C., more preferably 25 to 40 ° C., and stirring may be performed for 1 to 7 days. Further, molecular sieves may be added to the reaction solution. By this manufacturing method, MEL added as a material becomes a triacyl body almost quantitatively.

  The purification of triacyl MEL can be performed according to the above-described purification of MEL.

(Method for producing MEL having saturated side chain)
MEL having a saturated side chain can be produced in accordance with the above-described method for producing biosurfactant. At that time, as the fats and oils used as a raw material, it is carried out by adding one or more water-insoluble substrates such as olive oil, saturated fatty acids or esters thereof, hydrocarbons such as liquid paraffin and tetradecane.
The MEL having a saturated side chain can be purified according to the above-described MEL purification.

(Manufacturing method of MAL such as MML)
The production of MAL having alditol other than erythritol, such as MML, can be carried out according to the above-described method for producing biosurfactant. In that case, as a raw material, it carries out by adding alditol, such as mannitol, arabitol, ribitol, sorbitol, alone or in combination.
Purification of MAL having an alditol other than erythritol such as MML can be performed according to the above-described purification of MEL.

(Method for obtaining liposome)
Biosurfactants may be dissolved in oil-soluble bases or oil-soluble ingredients and blended in cosmetics, and in the form of liposomes, are preferably blended in cosmetics, particularly water-based cosmetics such as lotions and moisturizing liquids. . Biosurfactants blended in liposomes are preferable because they are fused with skin cells to improve absorbability. As a method for preparing the liposome, any known preparation method may be adopted, and it is not particularly limited. Examples thereof include an ethanol injection method and a bangham method.

For example, the preparation of the MEL-B liposome solution by the ethanol injection method may be performed as follows. That is, 10 mg of MEL-B is dissolved in 0.5 ml of ethanol, 1 mL of distilled water warmed to about 70 ° C. in advance is added and shaken gently, and then the remaining ethanol is distilled off with a rotary evaporator. This can be sonicated for about 5 minutes using a water bath type ultrasonic device (W-220R, Honda Electronics Co., Ltd.) and then diluted to 1 ml with distilled water.

For example, the preparation of the MEL-B liposome solution by the Bangham method may be performed as follows. That is, 10 mg of MEL-B may be dissolved in 1 ml of chloroform, and the solvent may be distilled off with a rotary evaporator to form a thin film. What is necessary is just to add 1 ml of distilled water there and sonicate for about 5 minutes using a water bath type ultrasonic device (W-220R, Honda Electronics Co., Ltd.).

(Method for producing cosmetic composition containing biosurfactant)
The addition amount of the biosurfactant in the cosmetic composition may be added within a range that does not impair the action of the biosurfactant, and is usually preferably 0.001 to 50% by mass, more preferably 0.1 to 20% by mass. -15% by mass is more preferable, and 3-10% by mass is particularly preferable. Here, the use form of the biosurfactant added to the cosmetic composition is arbitrary. For example, the biosurfactant may be used as it is as an extract from the culture solution, or after being purified, purified or suspended in water, or dissolved in an organic solvent such as ethanol.

(Humectant)
The moisturizing agent may be a hydrophilic moisturizing agent and / or a lipophilic moisturizing agent. As the hydrophilic moisturizing agent, amino acids, peptides, proteins, higher alcohols and derivatives thereof, and as the lipophilic moisturizing agent, phospholipid, sugar. Lipids and steroids may be used.

  As moisturizer, sodium pyridonecarboxylate, glycol, glycerin, glucose, maltose, maltitol, sucrose, fructose, xylitol, sorbitol, maltotriose, threitol, erythritol, amylolytic sugar-reducing alcohol, sorbitol, polyhydric alcohols Is ethylene glycol, 1,4-butylene glycol, diglycerin, triglycerin, tetraglycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1,3-propanediol, other serine, glycine, Threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate hepa Glycerophospholipid, glyceroglycolipid, sphingophospholipid, sphingoglycolipid, linoleic acid or its esters, eicosapentaenoic acid or its esters, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium Culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yokuinin extract, siamese extract, typhoid extract, diatomaceous earth extract, kidachi aloe extract, burdock extract, mannenrou Extract, Arnica extract, wheat bran, pyrrolidone carboxylic acid, tomato extract, camellia oil, soybean phospholipid, hyaluronic acid, threonine, ammonium glycolate, methylsilanol alginate, yocuinine, toki extract, toki root extract , Soybean extract, asparagus extract, DNA-Na, PCA-Na, RNA-Na, ashitaba extract, aspartic acid, amatya extract, alanine, arginine, alginate sodium, altea extract, aloe vera extract, oyster extract, barley extract, oyster leaf, Hydrolyzed keratin, hydrolyzed collagen, hydrolyzed conchiolin, hydrolyzed eggshell membrane, hydrolyzed egg white, hydrolyzed silk, hydrolyzed soy protein, brown algae extract, karin extract, raspberry extract, xylitol, chitosan, cucumber extract, cucumber fruit extract, Guava confectionery extract, quince seed extract, glycine, glycerin, glucose, grapefruit extract, grapefruit fruit extract, clematis extract, burdock extract, rice fermented liquor, chondroitin sulfate Na, fish colageno, sanza Extract, Shiitake extract, Giant extract, Glycerin, Cystine, Cysteine, Horsetail extract, Mallow extract, Serine, Sorbitol, Soy protein, Tomato extract, Lactic acid Na, Lactobacillus, Soybean fermented extract, Urea supine / Novara extract / Almond koji, Corn oil, Honey, hyaluronic acid Na, fukuno extract, betaine, loofah extract, maltitol, maltose, mannitol, lily extract, lactoferrin, lysine, apple extract, lotus extract, royal jelly, lanolin fatty acid polyethylene glycol 1000, lanolin fatty acid isopropyl, isopropyl myristate, myristic acid Isobutyl, hexyldecanol, myristyl lactate, lanolin fatty acid, tricaprylglyceryl, oleyl alcohol, octyldodecyl oleate Decyl oleate, reduced lanolin, octyldodecanol, almond oil, avocado oil, olive oil, oleic acid, orange rough oil, cocoa butter, carrot extract, sesame oil, sasanqua oil, safflower oil, dihydrocholesterol, squalane, cholesteryl stearate , Ceramide 2, evening primrose oil, sunflower oil, sunflower oil, cefnide 3, sunflower seed oil hybrid sunflower oil, phytosphingosine, grape seed oil, jojoba oil, jojoba seed oil, mineral oil, mink oil, macadamia nut oil, meadow foam oil, Examples include yuri oil, eucalyptus leaf oil, lanolin, linoleic acid, rosehip oil, petrolatum, and polyglutamic acid.

These humectants can be used alone or in combination of two or more.

(UV absorber)
As UV absorbers, use benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, benzophenone UV absorbers, etc. Also good.

For example, as an ultraviolet absorber, amyl benzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, benzyl cinnamate, paramethoxycinnamate 2 -Ethoxyethyl, octyl paramethoxycinnamate, glyceryl mono-2-ethyl hexanoate diparamethoxycinnamate, isopropyl paramethoxycinnamate, diisopropyl diisopropyl cinnamate ester, urocanic acid, ethyl urocanate, hydroxymethoxy Benzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonic acid Sodium, dihydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4′-methoxy-dibenzoylmethane, 2,4,6-trianilino-p- (carbo-2′-ethylhexyl-1′-oxy) -1, 3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole, 2-ethylhexyl paradimethylaminobenzoate, ethyl paraaminobenzoate, topylmethoxydipenzoylmethane, oxybenzone-1, ethyl guaiazulenesulfonate Examples of zinc oxide, sinoxate, and anthranilic acid-based ultraviolet absorbers include homomenthyl-N-acetylanthranylate.

These ultraviolet absorbers can be used alone or in combination of two or more.

(Antimicrobial agent)
The antibacterial agent may be an organic antibacterial agent and / or an inorganic antibacterial agent.

For example, as an antibacterial agent, buckwheat extract, halocarban, chlorophenesin, lysozyme chloride, alkyldiaminoethylglycine hydrochloride, isopropylmethylphenol, thymol, hexachlorophene, berberine, thioxolone, salicylic acid and their derivatives, benzoic acid, sodium benzoate , Paraoxybenzoic acid ester, parachlormetacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, carboxylic acid, sorbic acid, potassium sorbate, hexachlorophene, chlorhexidine chloride, trichlorocarbanilide, photosensitizer, bis (2-pyridylthio) -1-oxide) zinc, thianthol, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate Phenoxyethanol, resorcin, azulene, salicylic acid, zinc pyrithione, sodium mononitroguayacol, fennel extract, pepper extract, cetylpyridinium chloride, etc. benzethonium chloride and undecylenic acid derivatives.

These antibacterial agents can be used alone or in combination of two or more.

(Method of using cosmetic composition containing biosurfactant)
The form of the cosmetic composition containing the biosurfactant is not particularly limited. Therefore, it may take any state such as solid, liquid, paste, jelly, and powder. In order to form such a state, it can be solidified using, for example, a gelling agent, or can be dispersed using a liquid. Moreover, a solvent can be added to make a solution, or it can be spray-dried to form a powder.

The cosmetic composition containing the biosurfactant of the present invention has an excellent moisturizing effect and skin roughening preventing effect, and it has been confirmed that the skin surface is moderately moisturized and smooth. That is, the cosmetic composition containing the biosurfactant of the present invention can retain the moisture of the skin for a long time. Therefore, the cosmetic composition containing the biosurfactant of the present invention can be used very effectively when it is necessary to improve rough skin, improve keratin or protect the skin.

For example, a cosmetic composition containing the biosurfactant of the present invention can be used as a cosmetic. For example, cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair coloring, perfume, powder, pack, cleansing cream, shaving Cream, whisk, lotion, suntan oil, sunscreen oil, sunscreen lotion, sunscreen lotion, sunscreen cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, nail enamel, beautiful nail enamel It can be used as a remover, hair wash, bath cosmetic, lipstick, lip balm, eyeliner, toothpaste, deodorant, eau de cologne, hair nourishing agent, and hair restorer. The cosmetic composition containing the biosurfactant of the present invention can also be used as an ointment or a poultice.

Various components other than biosurfactants, moisturizers, ultraviolet absorbers and antibacterial agents can be further added to the cosmetic composition containing the biosurfactant of the present invention depending on the purpose of use.

For example, in a cosmetic composition containing a biosurfactant, the biosurfactant is highly hydrophobic, so it is dissolved in a nonionic surfactant, a lower alcohol, a polyhydric alcohol, or a natural oil such as olive oil, squalane, or a fatty acid. May be.

  Nonionic surfactants include, for example, sorbitan fatty acid esters (for example, sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate). Glycerol, penta-2-ethylhexyl diglycerol sorbitan, tetra-2-ethylhexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (eg mono cottonseed oil fatty acid glycerin, monoerucic acid glycerin, sesquioleate glycerin, glyceryl monostearate) , Α, α′-oleic acid pyroglutamate glycerin, monostearate glycerin malate, etc.); propylene glycol fatty acid esters (for example, monos Propylene glycol tea acrylate, etc.); hardened castor oil derivative; glycerin alkyl ether and the like.

  Examples of the POE-based hydrophilic nonionic surfactant include POE-sorbitan fatty acid esters (for example, POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate). POE sorbite fatty acid esters (eg, POE-sorbite monolaurate, POE-sorbite monooleate, POE-sorbite pentaoleate, POE-sorbite monostearate, etc.); POE-glycerin fatty acid esters (eg, POE-glycerol monostearate, POE-glycerin monoisostearate, POE-monooleate such as POE-glycerol triisostearate, etc.); POE-fatty acid esters (for example, POE-distearate) POE-alkyl ethers (for example, POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether), POE-monodiolate, ethylene glycol distearate, etc. POE-cholestanol ether, etc.); Pluronic type (for example, Pluronic, etc.); POE • POP-alkyl ethers (for example, POE • POP-cetyl ether, POE • POP-2-decyltetradecyl ether, POE • POP−) Monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether, etc.); tetra-POE / tetra-POP-ethylenediamine condensates (for example, Tetronic, etc.); POE-castor oil-cured castor Oil derivatives (eg POE-castor oil, POE-hardened castor oil, POE-hardened castor oil monoisostearate, POE-hardened castor oil triisostearate, POE-hardened castor oil monopyroglutamic acid monoisostearic acid diester, POE -Hardened castor oil maleic acid, etc.); POE-beeswax lanolin derivatives (eg POE-sorbite beeswax etc.); alkanolamides (eg coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide etc.); POE- Examples thereof include propylene glycol fatty acid ester; POE-alkylamine; POE-fatty acid amide; sucrose fatty acid ester; alkylethoxydimethylamine oxide; trioleyl phosphate.

  Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutyl alcohol, t-butyl alcohol and the like.

  Examples of the polyhydric alcohol include divalent alcohols (for example, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, Pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, octylene glycol, etc.); trivalent alcohol (eg, glycerin, trimethylolpropane, etc.); tetravalent alcohol (eg, 1, 2, 6) Pentaerythritol such as hexanetriol, etc .; pentavalent alcohol (eg, xylitol, etc.); hexavalent alcohol (eg, sorbitol, mannitol, etc.); polyhydric alcohol polymer (eg, diethylene glycol, dipropylene glycol, Tylene glycol, polypropylene glycol, tetraethylene glycol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol) Monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono 2-methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol Dibutyl Dihydric alcohol alkyl ethers (for example, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol) Monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol Dibutyl alcohol ether ester (for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diazebate, ethylene glycol disuccine) , Diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.); glycerin monoalkyl ether (example) Sugar alcohol (eg, sorbitol, maltitol, maltotriose, mannitol, sucrose, erythritol, glucose, fructose, starch-degrading sugar, maltose, xylitol, starch degradation) Sugar-reduced alcohol, etc.); Glysolid; Tetrahydrofurfuryl alcohol; POE-Tetrahydrofurfuryl alcohol; POP-Butyl ether; POP / POE-Butyl ether; Tripolyoxypropylene glycerin ether; POP-glycerin ether; POP-glycerin ether phosphate POP · POE-pentane erythritol ether, polyglycerin and the like.

  Oils include avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanca oil, castor oil , Linseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil Animal and vegetable oils such as beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane and their hardened oils. There are mineral oils such as liquid paraffin and petrolatum, and synthetic triglycerins such as glycerin tripalmitate.

  Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecylic acid, tallic acid, eicosapentaenoic acid, docosahexaene. There are acids. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathececinol, cholesterol, phytosterol, and isostearyl. There is alcohol. Synthetic esters include, for example, cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl orenate, dimethyl octanoate, cetyl lactate, myristyl lactate, etc. There is. Examples of silicone include linear polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and three-dimensional network structures such as silicone resins.

  As described above, the cosmetic composition containing the biosurfactant according to the present invention is preferably incorporated into a quasi-drug, a medical product, a hygiene product, and a pharmaceutical product.

  When the cosmetic composition containing the biosurfactant according to the present invention is implemented in the form of a quasi-drug, medical product, hygiene product, or pharmaceutical product, the dosage form is not limited, and ampoules, capsules, powders, granules, pills , Tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, mousses, bathing agents, and the like.

  Specifically, quasi-drugs, medical supplies, sanitary products, and pharmaceuticals include basic cosmetics such as internal and external pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, and facial cleansing agents. And skin cleansers, shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling agents, permanents, hair nickings, hair dyes, hair growth and hair restorations, foundations, white powder, white hair, lipstick, Makeup cosmetics such as blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, cosmetics for finishing such as nail care, perfumes, bath preparations, toothpastes, mouth fresheners, gargles, liquid odors -Examples include deodorants, sanitary products, sanitary cotton, and wet tissue. More specifically, cosmetic soap, shampoo, facial cleanser, rinse, eye cream, eye shadow, cream / milky lotion, lotion, perfume, funny, cosmetic oil, hair cosmetics, hair dye, paste perfume, powder, pack, Cleansing cream, shaving cream, bearded lotion, suntan oil, sunscreen oil, sunscreen lotion, sunscreen lotion, sunscreen cream, sunscreen cream, foundation, powdered perfume, blusher, mascara, eyebrow, nail cream, beauty nail enamel , Nail enamel remover, hair wash, cosmetics for bath, lipstick, lip balm, eyeliner, toothpaste, deodorant agent, cologne, hair nourishing agent and hair restorer. The cosmetic composition containing the biosurfactant of the present invention can also be used as an ointment or a poultice.

  The cosmetic composition containing the biosurfactant according to the present invention is suitably applied to humans, but can also be applied to animals other than humans as long as each effect can be expected.

  The cosmetic composition containing the biosurfactant according to the present invention contains ingredients and additives used in quasi-drugs, medical products, hygiene products, and pharmaceuticals as long as the effects of the present invention are not impaired as necessary. It can mix | blend together.

  Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

  Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane, and pristane.

For example, fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil,
Examples include natural fatty acids such as lanolin fatty acid, and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

  For example, as alcohols, natural alcohols such as ethanol, isopropylanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol, etc. , Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene Glycol, glycerin Batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, polyhydric alcohols such as sucrose and the like.

  For example, esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, lactic acid Examples include myristyl, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

  For example, examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylate.

  For example, gum and water-soluble polymer compounds include gum arabic, benzoin gum, danmar gum, guaiac oil, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, Carboxyalkylchitin, chitosan, hydroxyalkylchitin, low molecular chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose , Sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose Scan, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

  For example, as the surfactant, anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant ( Carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester) Type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, and the like.

  For example, vitamins such as retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (vitamin B1) ), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C, ascorbic acid and its derivatives, vitamin D In the group, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, in vitamin E group, tocopherol and its derivatives, ubiquinones, in vitamin K group, phytonadione (vitamin K1), me Naquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4) and the like.

  For example, amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, and the like, and sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

  For example, as an extract or extract of an animal or plant, herbal medicine, Asenyaku (Asen Yaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Achacha (Amacha), Aloe, Aloe Vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel (cage), turmeric (red gold), usba saishin (spicy), Japanese apricot (salmon plum), Japanese white beetle, waurusushi, green rose (fruit), hikiokoshi (life-prolonging grass), Japanese oak (yellow cocoon), Koganebana (Ogon), Yamazakura ( Cherry bark), yellowfin, yellow orchid, ginseng, carrot, helicopter, licorice, Dutch pepper, orange, sagebrush (long-distance), crabs (summer hay), tsurudokudami (what neck shark), enju (槐花）, mugwort (guy leaf), scallops (莪 朮), kudu (kazune), kanoko Cormorant (Valgarine), Chamomile, Kikarasuuri (Gurone), Kawara-momogi (Cervus chinensis), Licorice (licorice), Fukidan Popo (Cavity Winter Flower, Clover Winter Leaves), Raspberry, Kiwi Fruit, Japanese Clover (Kikkyo), Chrysanthemum (Chrysanthemum Flower) ), Catalpa (fruit), Citrus fruit (fruit), Tachibana (tachibana skin), Cucumber, Udo or Shishido (fruitful, solitary), apricot, wolfberry (bone crust, coconut, coconut leaf) , Clara, bittersweet, Kumazasa, grapefruit fruit, Nikkei (Kei-Kai), Keigai (Kai-Gai), Ebisugua (Keiko), Maruba morning glory or morning glory (Ken cow), safflower (Safflower), Gobaishi (Pyper), Confrey , Copaiba, gardenia, gentian, honoki (shock), hinata kozuchi (cow knee), goshuyu (burdock), burdock, kosen Garbage, rice, rice bran, wheat, Mishima Saiko (Saiko), saffron, savonso, hawthorn (Yamazako), salamander, salvia, sanchinin ginseng (37 ginseng), shiitake (shiitake), Jiou (geo yellow), Sikunshi (messenger), Murasaki (purple root), perilla (purple leaves, purple soko), oysters (柿蒂), peonies (glaze), psyllium (car forerunner, car front grass), ginger (ginger), Shobu (Japanese cypress), Japanese horned potato (Sadako), Shimotsuke, Shiraba, Honeysuckle (Gin and silver flowers, Shinobu), Ivy, Achillea millefolium, Elderberry, Azuki (Red Azuki), Elderberry (Bone), Zeniaoi, Senkyu ), Assembly (this medicine), mulberry (mulberry white skin, mulberry leaf), jujube (daegu), soybean, taranoki, chiketsu carrot (bamboo ginseng), Eggplant (Welcome mother), Warmoko (earth), Dokudami (10 medicines), Fuyumushinatsusatake (Cycium caterpillar), Pepper, Japanese physalis (Torone), Tachikakuso, Ryokucha (green tea), Kocha (tea), Choji (chome) ), Citrus unshiu (Chen bark), camellia, camellia, capsicum (Sanban), Touki (Tochigi), red bellflower, Daidai (Orange bark), Warmoko (dianthus), corn (Southern fur), Tochu (Tochu, Tochu) Leaf), tomato, Nanten (Southern fruit), garlic (large sun), barley (malt), hakusen (white birch), janohi (barley winter), parsley, grasshopper, mint (light load), hamamelis, rose, loquat Leaves (bamboo leaves), Matsuhodo (bamboo leopard), grapes or their leaves, loofah, bodaiju, buttons (peony skin), hops, maikai (my buds), pine Leaves, Marronnier, Mannenrou, Mukuroji, Melissa, Merirot, Bokeh (Kiso), Palms, Peach (Momojin, Peach), Hagi (Giraffe), Binroju (Mariko), Mejijiki (Masame), Cornflower, Yukinoshita ), Bayberry (Yellow Plum), Yashabushi (Yagari), pearl barley (Yokuinin), mugwort, sagebrush, lavender, apple fruit, garlic mushroom (reishi), lemon fruit, forsythia (ream), forsythia, ginkgo biloba Hasidorokoro (roth root), chicken tosaka, bovine / human placenta extract, pig / cow stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin , Elastin hydrolyzate, water-soluble elastin derivative, silk protein, silk protein Hydrolyzate, and the like bovine hemocyte protein hydrolyzate.

  Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder. .

  For example, examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

  For example, inorganic pigments include anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow oxide Examples include iron, bengara, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, and calamine.

  For example, astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid Etc.

  Examples of the bactericidal / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

  For example, selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thianthol, castari tincture, ginger tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate And thioglycolic acid.

  For example, as perfumes, natural animal perfumes such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, Iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil , Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, bell Vegetable fragrances such as Mott essential oil, Peruvian balsam essential oil, Boad Rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese seed mint essential oil, Other synthetic fragrances can be mentioned.

  For example, as a dye / colorant, a red cabbage dye, a red rice dye, an akane dye, an anato dye, an squid dye, a turmeric dye, an enju dye, a krill dye, an amber dye, a caramel, a gold, a silver, a gardenia dye, a corn dye, Examples include onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment and rutin.

  Examples of sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.

  For example, examples of the nutrient fortifier include shell calcined calcium, cyanocolabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.

  Others, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, refreshing agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides and their Degradation product, animal / plant glycoprotein and its degradation product, blood flow promoter, anti-inflammatory agent / antiallergic agent, cell activator, keratolytic agent, wound treatment agent, foam enhancer, thickener, oral preparation, Deodorizing / deodorizing agents, bittering agents, seasonings, enzymes and the like.

[Anti-aging method]
By using the cosmetic composition containing the biosurfactant according to the present invention, an anti-aging method can be provided. That is, the present invention includes an anti-aging method using a cosmetic composition containing a biosurfactant.

  The anti-aging method according to the present invention is characterized by including (i) a step of bringing a biosurfactant into contact with an animal. Biosurfactants include mannosyl erythritol lipid (MEL), mannosyl alditol lipid (MAL) (for MAL, mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), mannosyl ribitol lipid (MAL). MRL), at least one selected from the group consisting of a triacyl form of mannosyl erythritol lipid (MEL) and a triacyl form of mannosyl alditol lipid (MAL).

  The animal is not particularly limited as long as the anti-aging effect of a cosmetic composition containing a biosurfactant can be expected, but mammals are preferable, and humans are particularly preferable.

  “Contacting an animal” is intended to contact a cosmetic composition containing a biosurfactant to the extent to which an external preparation is applied, such as the skin and mucous membrane of an animal.

  The anti-aging method according to the present invention is characterized in that it further comprises (ii) a step of activating cells with a cosmetic composition containing a biosurfactant. By activating cells with a cosmetic composition containing a biosurfactant that has a cell activation effect, for example, preventing and improving skin wrinkles, sagging, hardening, etc., and maintaining an elastic, youthful and healthy skin state It is possible to exert anti-aging effects such as.

The cosmetic composition containing the biosurfactant of the present invention can be widely used when a moisturizing effect, a collagen production promoting effect, a cell activation effect, an anti-aging effect, a hair-growth effect and a rough skin improving effect are required. The amount to be used is determined within a range in which the desired wet effect, collagen production promoting effect, cell activation effect, anti-aging effect, hair-growth effect, and rough skin improving effect are sufficiently exhibited.

Since the cosmetic composition according to the present invention is used in anticipation of the above-described effects, it is of course used as a cosmetic composition or a cosmetic product. It can be used in the form of medical supplies, hygiene supplies, pharmaceutical compositions, functional food compositions, quasi-drugs, medical supplies, hygiene supplies, pharmaceuticals, and premixed products. .

  It should be noted that the specific embodiments and the following examples made in the section of the best mode for carrying out the invention are intended to clarify the technical contents of the present invention, and to such specific examples. It is not to be construed as limiting in any way whatsoever, and those skilled in the art can implement the invention within the spirit of the invention and the scope of the appended claims.

The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.
(Method for evaluating rough skin improvement)
Test tissue (Toyobo Co., Ltd.) LSE-002 or 003 kit is taken out according to the instructions of the instructions attached to the 003 kit. A ring that secures the drug exposure site is adhered to the surface of the LSE tissue, and a 1% aqueous solution of sodium dodecyl sulfate (SDS) is added to the ring and left at room temperature for 5 minutes. Thereafter, SDS was removed with an aspirator, and 3 ml of the assay medium was sprayed with a pipette and washed. By this operation, the moisturizing component of the stratum corneum was eluted and a dry skin was created.

Next, the test substance solution was added to the surface of LSE (Living Skin Equivalent) tissue with each 70 μl micropipette. Subsequently, the LSE tissue was placed in an assay tray containing assay medium in a CO ₂ incubator adjusted to a temperature of 37 ° C. and a relative humidity of 15% RH to 20% RH, and incubated for 24 hours. Thereafter, the LSE tissue is removed from the CO ₂ incubator, and 0.6 ml of the assay medium mixture containing 0.333 g / ml of tetrazolium salt (MTT) reagent is added to the assay tray in accordance with the instructions attached to the LSE-002 kit. And incubated in a CO ₂ incubator adjusted to a temperature of 37 ° C. and a relative humidity of 15% RH to 20% RH for 3 hours.

  After the MTT treatment, the central portion of the LSE tissue was cut out together with the polycarbonate film using an 8 mmφ biopsy punch, the section was transferred to a small test tube, 700 μl of 0.04N hydrochloric acid-isopropanol was added, and the mixture was extracted in the dark for 2 hours. After completion of extraction, the mixture was stirred and thoroughly mixed, and the absorbance of the extracted blue-violet formazan was measured at 562 nm. The absorbance obtained by this method and the rough skin improvement effect are closely related, and it is an effective rough skin improvement evaluation method capable of quantitatively, simply and economically evaluating the rough skin condition of humans.

Example 1 Production of MEL-A and Triacyl MEL-A Inoculum culture was performed by inoculating Pseudozyma antarctica NBRC 10736 in a seed medium (20 ml / 500 ml Sakaguchi flask) for 1 loop. Cultured overnight at 30 ° C. The obtained culture broth was used as an inoculum. The composition of the seed medium was 4% Glucose, 0.3% NaNO3, 0.02% MgSO4 · H2O, 0.02% KH2PO4, 0.1% yeast extract. For the culture, 75 ml of the above inoculum was inoculated into 1.5 L (5 L-jar) of the production medium and cultured using 5 L-jar under the conditions of 30 ° C., 300 rpm (stirring rotation), 0.5 L / min0 (Air). The composition of the production medium was 3% soybean oil, 0.02% MgSO4 · H2O, 0.02% KH2PO4, and 0.1% yeast extract. 250 ml of the culture solution was centrifuged (6500 rpm, 30 min), the supernatant was removed, and the precipitate (bacteria) was collected. 50 ml of ethyl acetate was added to the precipitate, and after sufficient stirring, the mixture was centrifuged (8500 rpm, 30 min), separated into a precipitate and a supernatant, and the supernatant was concentrated with an evaporator. Elution with hexane: acetone = 5: 1 and hexane: acetone = 1: 2 was performed using silica gel to obtain MEL fractions (MEL-A, MEL-B, MEL-C) and triacyl MEL-A fractions.

Example 2 Preparation of MEL-B and triacyl MEL-B
0.2 ml of P. tsukubaensis frozen stock was inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C., 180 rpm, and used as a seed seed. 0.2 ml of the inoculum was again inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to obtain an inoculum. 20 ml of inoculum was inoculated into 2 L of YM medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture was centrifuged at 7,900 rpm for 60 min at 4 ° C. to separate the cells (including MEL-B) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-B crude purified product was eluted with hexane: acetone using a silica gel column to obtain MEL-B and triacyl MEL-B fraction purified products.

Example 3 Preparation of MEL-C and triacyl MEL-C
0.2 ml of P. hubeiensis frozen stock was inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C., 180 rpm, and used as a seed seed. 0.2 ml of the inoculum was again inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to obtain an inoculum. 20 ml of inoculum was inoculated into 2 L of YM medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture was centrifuged at 7,900 rpm for 60 min at 4 ° C. to separate the cells (including MEL-C) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-C crude purified product was eluted with hexane: acetone using a silica gel column to obtain MEL-C and triacyl MEL-C fraction purified products.

Example 4
In the production of MEL in Example 1, soybean oil was used as a raw material for production. Instead, olive oil was used and cultured in the same manner as in Example 1 to isolate MEL-A, MEL-B, and MEL-C. Purify. The MEL fraction obtained at this time is called MEL-A (OL), MEL-B (OL), and MEL-C (OL) in order to distinguish from the MEL of Example 1.

Example 5 Mannosyl mannitol lipid (MML) production Pseudozyma parantarctica JCM 11752 strain was cultured. That is, Pseudozyma parantarctica JCM 11752 strain preserved in a preservation medium (malt extract 3 g / L, yeast extract 3 g / L, peptone 5 g / L glucose 10 g / L, agar 30 g / L) In a test tube containing 2 mL of liquid medium having a composition of glucose 20 g / L, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L, and magnesium sulfate 0.3 g / L. One platinum loop was inoculated and cultured with shaking at 30 ° C. for 1 day. Subsequently, the obtained bacterial cell culture solution was mixed with olive oil 50 g / L as a vegetable oil, mannitol 100 g / L as a sugar alcohol, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L. And a Sakaguchi flask containing 30 mL of a liquid medium having a composition of magnesium sulfate 0.3 g / L, and cultured at 34 ° C. for 7 days. The above culture broth was collected, the mannosyl alditol lipids in the broth were extracted with ethyl acetate, purified by silica gel chromatography, and the structure of the product was analyzed by ¹³ C NMR and ¹ H NMR.

Example 6 Production of mannosyl arabitol lipid (MAraL) MAraL was produced by culturing in the same manner as in Example 5 except that culture in Example 5 was carried out in a medium containing 100 g / L of arabitol as a sugar alcohol.

Example 7 Production of Mannosyl Sorbitol Lipid (MSL) MSL was produced by culturing in the same manner as in Example 5 except that the culture of Example 5 was carried out in a medium containing 100 g / L of sorbitol as a sugar alcohol.

Example 8 Production of mannosyl ribitol lipid (MRL) MRL was produced by culturing in the same manner as in Example 5 except that cultivation of Example 5 was carried out in a medium containing 100 g / L of ribitol as a sugar alcohol.

Example 9: Manufacture of MEL-A serum containing moisturizer A serum having the composition shown below was manufactured by a conventional method. As a control, a serum containing no MEL-A was also produced by a conventional method.
(Composition) (wt%)
Oil layer component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
MEL-A 5.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 10: Production of MEL-A beauty essence containing ultraviolet absorber and humectant A beauty essence having the composition shown below was produced in a conventional manner. As a control, a serum containing no MEL-A was also produced by a conventional method.
(Composition) (wt%)
Oil layer component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
MEL-A 5.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Ultraviolet absorber: Octyl paraaminobenzoate 4.0
Moisturizer: Polyglutamic acid 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 11: Production of MEL-A serum containing anti-humectant and antibacterial agent A serum having the composition shown below was produced by a conventional method. As a control, a serum containing no MEL-A was also produced by a conventional method.
(Composition) (wt%)
Oil layer component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
MEL-A 5.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Anti-humectant: polyglutamic acid 4.0
Antibacterial agent: Octyl paraaminobenzoate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 12: Manufacture of MEL-A serum containing ultraviolet absorber, moisturizer, and antibacterial agent A serum having the composition shown below was prepared by a conventional method. As a control, a serum containing no MEL-A was also produced by a conventional method.
(Composition) (wt%)
Oil layer component: stearic acid 3.0
Squalane 5.0
Olive oil 5.0
MEL-A 5.0
Emulsifier: POE sorbitan monolaurate 5.0
Sorbitan monostearate 5.0
Alkali: Triethanolamine 1.0
Moisturizer: Polyglutamic acid 4.0
UV absorber: CoQ10 4.0
Antibacterial agent: zinc undecylenate 4.0
Aqueous phase component: propylene glycol 5.0
Total amount of purified water is 100

Example 13 Production of MEL-B cosmetic liquid MEL-B was prepared in the same manner as in Examples 9-12 except that MEL-B was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12. A serum was produced.

Example 14 Production of MEL-C cosmetic liquid MEL-C was prepared in the same manner as in Examples 9-12 except that MEL-C was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12. A serum was produced.

Example 15 Production of MML Cosmetic Liquid MML cosmetic liquid was produced in the same manner as in Examples 9-12 except that MML was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12.

Example 16 Manufacture of MAraL essence In the manufacture of essences of Examples 9-12, MAraL essence was prepared in the same manner as in Examples 9-12, except that MAraL was used instead of MEL-A.

Example 17 Manufacture of MSL serum liquid MSL serum liquid was manufactured in the same manner as in Examples 9-12 except that MSL was used instead of MEL-A in the manufacture of the serum liquids of Examples 9-12.

Example 18 Production of MRL serum A MRL serum was produced in the same manner as in Examples 9 to 12 except that MRL was used instead of MEL-A in the production of serums of Examples 9 to 12.

Example 19 Production of Triacyl MEL-A Cosmetic Liquid Triacyl MEL-A was prepared in the same manner as in Examples 9-12 except that triacyl MEL-A was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12. A MEL-A serum was prepared.

Example 20 Production of Triacyl MEL-B Cosmetic Liquid Triacyl MEL-B was prepared in the same manner as in Examples 9-12 except that triacyl MEL-B was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12. A MEL-B serum was prepared.

Example 21 Production of Triacyl MEL-C Cosmetic Liquid Triacyl MEL-C was prepared in the same manner as in Examples 9-12 except that triacyl MEL-C was used instead of MEL-A in the production of the cosmetic liquids of Examples 9-12. A MEL-C serum was prepared.

Comparative Example 1
Using a serum that does not contain MEL prepared with the compositions shown in Examples 9 to 12, five panelists evaluated the spread, stickiness, moist feeling, and the difficulty of being bulky when using this. Judgment was made according to the following criteria. The results are shown in Table 1.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

Example 22
Using the serums of the compositions shown in Examples 9 to 12, five panelists evaluated the spread, stickiness, moist feeling, and hardness when used, and determined according to the following criteria: . The results are shown in Table 2. In any case, the moisturizing effect was evaluated as good.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

Example 23
Using the cosmetic liquids of the compositions shown in Examples 13 to 21, five panelists evaluated the spread, stickiness, moist feeling, and the difficulty of sticking when used, and determined according to the following criteria: . The results are shown in Tables 3 to 11. In any case, the moisturizing effect was evaluated as good.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

As is apparent from Tables 1 to 11, the products of the present invention were good in all of stretch, stickiness, moist feeling, and hardness.

Example 24
About the moisturizing effect when five panelists of the essence containing the MEL shown in Examples 9 to 12 use this, the essence containing no MEL prepared with the composition shown in Examples 9 to 12 was used. It evaluated as a comparative example. As apparent from Table 12, the product of the present invention was evaluated as having a good moisturizing effect.
Judgment criteria:
A: 4 or more out of 5 evaluated as good.
A: 3 out of 5 people evaluated it as good.
(Triangle | delta): Two persons evaluated that 5 persons were favorable.
X: 1 or less out of 5 evaluated as good.

The cosmetic composition containing the biosurfactant of the present invention has effects such as moisturizing action, collagen production promoting action, cell activating action, anti-aging action, hair growth action and rough skin improving action, and the like, cosmetics, quasi drugs, medical supplies Sanitary goods and pharmaceuticals can be provided, and since the raw material cost is low, mass production is possible, and it can sufficiently withstand long-term use, it is expected to contribute greatly to the industry.

Claims (14)

  A cosmetic composition comprising at least a biosurfactant and a humectant.   The cosmetic composition according to claim 1, wherein the biosurfactant has a mannose skeleton.   The cosmetic composition according to claim 1 or 2, wherein the biosurfactant has a sugar alcohol linked to a glycoside bond to the hydroxyl group at the 1-position of the mannose skeleton. The biosurfactant having a mannose skeleton is mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and / or mannosyl ribitol lipid (MRL) The cosmetic composition according to claim 3. MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), a triacyl derivative of MEL-A, The cosmetic composition according to claim 4, wherein the cosmetic composition is at least one compound selected from the group consisting of a triacyl form of MEL-B, a triacyl form of MEL-C, and a triacyl form of MEL-D. The cosmetic composition according to any one of claims 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid. The cosmetic composition according to any one of claims 1 to 6, wherein the biosurfactant forms a lamellar structure and / or a liposome. The cosmetic composition according to claim 7, wherein a coexisting active ingredient is embedded in a lamellar structure and / or liposome formed by a biosurfactant. The cosmetic composition according to any one of claims 1 to 10, wherein the humectant is a hydrophilic humectant and / or a lipophilic humectant. Furthermore, an ultraviolet absorber is contained, The cosmetic composition in any one of Claims 1-9 characterized by the above-mentioned. Furthermore, an antibacterial agent is contained, The cosmetic composition in any one of Claims 1-10 characterized by the above-mentioned. The UV absorber is selected from the group consisting of benzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, triazine UV absorbers, and benzophenone UV absorbers. The cosmetic composition according to claim 10, wherein the cosmetic composition is one or more compounds. The cosmetic composition according to claim 11, wherein the antibacterial agent is an organic antibacterial agent and / or an inorganic antibacterial agent. The cosmetic composition according to any one of claims 1 to 13, which is used as a cosmetic, a quasi-drug, a medical product, a hygiene product, or a pharmaceutical product.