JP2010001267A - Plant extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sesame extract as a new food material, cosmetic, and medical material which have a component derived from a natural substance as an active ingredient, accordingly has no concern for safety, exhibits an excellent effect on the improvement of skin roughness and the prevention of skin aging, and furthermore shows a never-known-before effect on the skin or the cutis. <P>SOLUTION: The sesame extract comprises, as active ingredients, sesame lignans of antioxidants, saturated fatty acids, unsaturated fatty acids, and enzymes which are derived from sesame seeds and present in sesame seeds as active ingredients. The sesame extract comprises not less than 0.9 wt.% sesamins in the extract of sesame seeds. The sesame extract is provided in the form of a sesame oil obtained by pressing sesame seeds at a low temperature of not higher than 30°C. The sesame extract is provided in the form of an extract of the sesame oil obtained by pressing sesame seeds at low temperatures. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

The present invention relates to a plant extract, and more particularly to a sesame seed-derived sesame lignan having an excellent effect in improving rough skin and preventing skin aging, and a plant extract containing an active ingredient of an unsaturated fatty acid and an enzyme. .

Conventionally, for the purpose of improving rough skin, preventing skin aging, and skin medical materials, foods such as beverages, dietary supplements, and edible oils, as well as various emulsions, creams, lotions, packs, cleaning agents, ointments, etc. Cosmetics are used. As a medicinal component of such cosmetics, a wide variety of medicinal components such as synthetic products, extracts from natural products, derivatives thereof and the like have been proposed, and in particular, there are many extracts from natural products in terms of safety. It has been proposed (see Patent Document 1).

However, extracts from these natural products have the disadvantage that the effects of medicinal ingredients are insufficient and the effects vary (see Patent Documents 1 to 3).
The first step is to promote clinical trial programs that are safe to apply and are not toxic after long-term use. Based on the report, histopathological, hematological, and immunological examinations are performed on the phenomena related to the occurrence of pruritus caused by long-term hemodialysis patients and long-term atopic patients. As a result, the results have been published (see Patent Documents 4 to 5).

JP 2000-119155 A, JP-A-11-180885 JP 10-45615 A Career Oil Dictionary (Author) Ren Price (Tokyodo Publishing) Dangerous oil causes disease (Author) Dr. S. Finnegan (Central Art Publisher)

  The problem to be solved by the present invention is an extract from natural special sesame, food such as beverages, health supplements, edible oils, and emulsions, creams, lotions, packs, cleaning agents, ointments, etc. Adds to various cosmetics to improve rough skin, prevent skin aging, and prevent itchiness. It is in the place to provide things.

  These fatty acids must be ingested because the body does not produce several types of fatty acids necessary to stay healthy. These fatty acids are said to be essential because they are very important. Among them, two unsaturated fatty acids, linoleic acid and linolenic acid, are contained in essential fatty acids. Each essential fatty acid is supplemented with prostaglandins that keep blood constant in the body and increase blood flow to internal organs to prevent blood clotting.

  However, the current production process of vegetable oil is accompanied by high heat and chemicals, and many natural properties are lost. Highly refined oils, in particular, have been pretreated with phosphorus, refined with alkali and vacuum bleached and deodorized using activated carbon, but those that have undergone these treatments are not adequate.

In addition, vegetable oil added with hydrogen produces a fatty acid called a trans type in the process of making some unsaturated fatty acids. In natural vegetable oils, one hydrogen atom of a carbon double bond is bonded to each other. However, when this becomes a partially hydrogenated fatty acid, the hydrogen atom on one side moves to the opposite side, so that part of the fatty acid molecule resembles a saturated fatty acid.

  The trans-bonded fatty acids that are produced during the refinery process by modern heating are the same as those produced by used fried oil. Such a trans fatty acid oil that does not exist in nature is not very desirable to use in the food and cosmetic fields.

  Moreover, it is a component extracted from a natural product that is sesame, and it can be extracted effectively without destroying the components of the plant contained therein, in particular, the enzyme, directly from the plant at a temperature of 30.degree. There is a need to extract oxidants. Furthermore, it is desired that such a stock solution is used in the fields of food materials, cosmetics, and medical materials.

It is a plant extract containing active ingredients of antioxidant sesame lignans, saturated fatty acids, unsaturated fatty acids and enzymes as food materials, cosmetics and medical materials.
It is a natural antioxidant containing sesamorin, sesamol, sesaminol, sesamin, sesamorinol, etc. in the form of a high concentration containing 0.9% by weight or more of sesame lignans as antioxidants.

Fatty acids include 20% by weight or less of the saturated fatty acids palmitic acid and stearic acid, 30% by weight or more of the unsaturated fatty acid oleic acid, 40% by weight of linoleic acid, and 1% by weight of α-linolenic acid. Thus, the total amount of unsaturated fatty acids is 80% or more. Preferably, the unsaturated fatty acid is 80-90%.
Enzymes are catalase, protease, and peptidase, and contain any one or more of these enzymes. It is effective to contain mainly protease.

  The active ingredients of antioxidant sesame lignans, saturated fatty acids, unsaturated fatty acids, and enzymes are separated from sesame oil obtained by squeezing sesame seeds produced in the savanna zone at a low temperature of 30 ° C. or lower. The pressing temperature is preferably 20 ° C to 10 ° C.

As a result of studies conducted by the present inventors to solve the above problems, it was found that sesame oil is a very stable oil due to the natural antioxidant effect of sasamol, sesamin and sesamolinol formed from sesamolin. It was also found that komagrinans crystallize in oil (content 0.98 g / 100 g) by reducing water as much as possible at high temperature.
It has been found that sesame seeds derived from sesame seeds and unsaturated fatty acids are correctly suitable as medicinal ingredients.

  At present, there is no method for squeezing raw sesame at a low temperature of 30 ° C. or less in a general production method. Sesame essential oil will not come out unless heat above 80 ℃ is applied. Although sesame oil is squeezed at such a high temperature, sesame lignans and unsaturated fatty acids are obtained, but their contents are also reduced and no enzyme components are collected. Therefore, such sesame oil does not provide a sufficient skin effect.

  It is also known that vegetable oils rich in essential fatty acids are effective for drying the skin. The stratum corneum of the skin is usually rich in saturated and unsaturated fatty acids, but the deficiency of these essential fatty acids increases transepidermal water loss (TEWL), resulting in dry skin. Since the enzyme converts linoleic acid (LA) to γ-linolenic acid (GLA), the lack of the necessary enzyme results in a low GLA content in the epidermis, and low linoleic acid LA in dry skin It has become.

  In addition, this sesame oil has an excellent antibacterial action, and when added to a culture group, it has been shown that the bacteria that grow is reduced by nearly 99%, and the bactericidal power of Staphylococcus aureus has also been recognized.

  INDUSTRIAL APPLICABILITY The present invention is a commercial product that is recognized to have an effect on itching related to food materials, cosmetics, and medical materials containing sesame seeds derived from sesame seeds, unsaturated fatty acids and enzymes as active ingredients.

Each of the 60 trillion cells that make up the human body is enveloped by a membrane, and each cell operates in its own social form and lives in its life sphere. Is maintained. That is, one cell is formed by a nuclear membrane that wraps the inner nucleus, a cell membrane that wraps the whole cell, and a cytoplasm between the nuclear membrane and the cell membrane.

  In the nucleus enveloped in the nuclear membrane, there are 23 pairs (64) of chromosomes in humans, and the genes that are active on life to maintain (design) human life on those chromosomes. (Tack four kinds of bases, namely adenine (A) cytosine (C) agnin (G) thymine (T) are linked like a long string, and sugar and phosphate form a support to form DNA is doing.

  The cell membrane that forms a single cell consists of a double lipid molecular layer, in which proteins (including enzymes) are interspersed, most of the cell membrane is phospholipids, cholesterol Is also included. Proteins that exist outside the cell membrane are selectors (receptors) that bind to microorganisms such as toxins, bacteria, and viruses, as well as hormones and chemicals necessary for cells.

  The skin that wraps the human body protects the substances necessary for the body from being broken and prevents harmful invasion from the outside. This skin is structurally formed by the epidermis and the dermis below it. Under the dermis, there is a subcutaneous tissue, and it has a function to adjust to shocks from outside and temperature changes. The epidermis has no blood vessels and is made up of several cell layers. The cell layer at the bottom of the epidermis is called the basal layer, and the cells are in contact with the dermis, and are supplied with nutrients that diffuse from there. ing.

  The cells of the basal layer of the epidermal cells repeat cell division, and millions of cells are newly made every day. A capillary network is developed in the epidermis, from which nutrients and enzymes are supplied to the epidermis. The vascular network abundantly distributed in the dermis is involved in the regulation of body temperature. When the body temperature rises, the capillaries of the skin expand, releasing heat from the body surface, and when the outside temperature falls, Do not let go.

  That is, there are receptors for cold nuclei, warm sensation, tactile sensation, pressure sensation, and pain sensation. As an important physiological aspect of the skin, many sensory nerves are distributed in the skin and receive external sensory stimuli (eg, temperature, pressure, pain), which are transmitted as information to the central nervous system centered on the brain. . We know that living organisms react to this information.

  There are individual differences in the occurrence and intensity of itch. Itching dries the skin due to lack of water in the body including blood. Among sweat glands, eccrine sweat glands play an important role in regulating body temperature and are susceptible to sympathetic nerves, and secrete a lot of sweat when the temperature outside the body is high and when the body temperature is high, but through the nervous system It is said to be easily affected by pain and stress, and is particularly related to the occurrence of itching.

It has become cosmetics and medical products that improve the quality of life (QOL) by improving the mental pain of pruritus associated with hemodialysis and the mental distress of pruritus associated with atopy.
There are many theories about the cause of the itching of the skin, but 1) the development of itching due to the oxidative phenomenon that occurs in the skin due to the retention substance locally due to lack of blood, 2) the abnormal reaction of the nervous system due to mental stress, 3 It has been proposed to use mast cells (mast cells) by applying some kind of stimulation to the skin.

  A cell that is not detected in the blood and is one of the basic spheres in the connective or mucosal tissue under the skin. The intracellular granules contain many kinds of scientific mediators. In particular, since there are selectors (receptors) that bind to IgE molecules (Fc part) among immune probrins on the membrane surface of mast cells, for example, IgE molecules are produced by substances that cause allergies (allergens). IgE cell molecules bind to the membrane surface. Mast cell degranulation reaction occurs when the previous allergen binds to 2 molecules of IgE on the membrane of mast cell, and allergic reaction occurs by releasing many kinds of scientific mediators including histamine It is said that.

  These reactions are thought to cause itch. This phenomenon may cause the release of substance P (SP) from the end of sensory nerve fibers. SP may cause histamine release from mast cells, vasodilation, crystal leakage, and may be involved in allergic reactions such as itching. It is considered.

  In addition to histamine, cetronin, neuropeptide, substance P, bradykinin, neurothecin, VIP, endorphins and the like are known as factors that cause itch. It is said that these substances bind to a receptor expressed on the cell membrane of mast cells and release histamine from the mast cells, so that itching is expressed.

Analysis of the sesame extract extracted by the present inventor reveals that the saturated fatty acids palmitic acid (C15H31CO2H) 9.4%, stearic acid (C17H35CO2H) 4.9%, hebenic acid (C12H43CO2H) 0.1%, glycerin (C23H47CO2H) 0.2%, further palmitoleic acid as unsaturated fatty acids (C16H30O ^2), oleic acid (C16H43O2) 39.9%, linoleic acid (C18H43O2) 44.3%, linolenic acid (C18H30O2) 1.0%, arachidonic acid (C20H32O2) 0.6%, Eikosein acid (C24H46O2) 0.2 % Is detected.

  Among the tissues of this sesame extract, saturated fatty acids palmitic acid, stearic acid or unsaturated fatty acids oleic acid, and linoleic acid suppress the expression of μ (mu) opioid peptides that induce itch in blood. On the other hand, it is considered that there is any action that promotes secretion of κ (kappa) opioid peptides that suppress itch. It is also considered that the antioxidant action by promoting the secretion of κ (kappa) opioid has the effect of suppressing itching.

Substance P (SP) is involved in the transmission of both pain and itch because it eliminates both pain and itching, similar to capsancin.
Fatty acids are constituents of cell membranes, but if the linear form of trans fatty acid molecules are mixed into the cell membrane, the cells become weak, resulting in various problems. We succeeded in the extraction of horseshoe-shaped cis-type fatty acid molecules, which are natural forms.

  Excellent in the effect of medicinal ingredients for improving rough skin and preventing skin aging, natural ingredient-rich fatty acids extracted with (sesamesis type fatty acid under 30 ° C raw squeezed) are a source of itching and pigmentation in hemodialysis patients and atopic patients Effective for erasure

In the present invention, it is a natural antioxidant containing sesamorin, sesamin, sesamol, sesamorinol, etc. in the form of a high concentration containing 0.9% by weight or more of the antioxidant sesame lignans. In general, sesame seeds contain about 0.1 to 0.9% by weight of sesame lignans.
In the present invention, sesame seeds were grown under severe conditions in a very dry zone, thereby succeeding in containing a high concentration of 0.9% by weight or more of sesame glycans.

Superoxide dismutase (SOD enzyme) and catalase activity are said to be extremely powerful active oxygen scavenging enzymes (antioxidants) that scavenge their active oxygen. In the body, harmful superoxide radicals generated as a result of metabolism must be removed. As a result, hydrogen peroxide is always produced in the cell (2 · O ₂ ⁻ + 2H ⁺ → O ₂ + H ₂ O ₂ ). At high concentrations, hydrogen peroxide is also harmful to cells.

The enzymes of the present invention are catalase, protease, and peptidase. Protease is a general term for enzymes that catalyze the hydrolysis of peptide bonds (-CO-NH-). Proteinase, peptidase, It is divided into.
Proteinase is an enzyme that hydrolyzes the peptide bond of protein molecules, and is also called endopepti-dase. When it acts on high molecular weight proteins, rapid depolymerization occurs, and proteins are peptone.

  Peptidase is an enzyme that hydrolyzes the peptide bond at the amino-terminus or carboxy-terminus of the peptide chain. It is also called exopeptidase and releases amino acids sequentially from the end of the peptide chain. In general, when referring to a protease, it often means a proteinase. An aspartic acid residue and an aspartic acid carboxylate ion are involved in the aspartic proteinase activity center, and include pepsin and an acidic protease (Orientase 20A) originating from Aspergillus niger.

  The essential oil of the sesame plant extract according to the present invention includes, in addition to foods such as beverages, health supplements, and edible oils, as basic cosmetics such as emulsions, creams, lotions, packs, and oils, And whole body cleaning, shampoo, rinse, hair treatment, hair cream, hair spray, hair tonic, hair styling, hair growth, hair restoration, etc., foundation, white powder, lipstick, mascara, eye shadow, eyeliner, eyebrow, Examples include makeup cosmetics such as beauty nails, odor control agents, and ointments. Examples of the form include solid, paste, mousse, gel, powder, solution, solubilization, emulsification, powder dispersion, and multilayer.

In the present invention, sesame glycans derived from sesame seeds, which are natural products, enzymes and fatty acids are used as active ingredients, so there is no concern about safety, and it has excellent effects in improving rough skin and preventing skin aging. There is an effect that it can be provided to food materials, cosmetics, and medical materials containing sesame essential oil that does not vary in effect.
Extracts from natural sesame special sesame are added to foods such as beverages, dietary supplements, edible oils, and various cosmetics such as emulsions, creams, lotions, packs, cleaning agents, ointments, etc. It was possible to provide medicinal ingredients for improvement, prevention of skin aging and prevention of itching.

Examples of the cosmetic according to the present invention include the following.

A method for producing a plant extract which is an essential oil of sesame is described.
300 parts of Sri Lankan sesame seeds were sun-dried and dried with a 1m diameter and 1m high mortar, and using a very hard wooden bar to squeeze sesame into the mortar at a low temperature of 15 The wooden stick was slowly rotated around the mortar with the drive power used, and the sesame sandwiched between the mortar and the stick was squeezed to obtain 100 parts of the squeezed extract.

  The temperature of the oil extracted from the die was measured by a platinum temperature sensor, and the rotational speed of the drive system was automatically controlled while monitoring this temperature. It was always possible to squeeze the oil at a constant temperature. Even when there was a change in the ambient temperature, oil at a constant temperature was extracted by controlling the rotation speed. The extracted oil was allowed to stand for 3 days in a stationary tank made of SUS. 60 parts of the golden extracted oil at the upper part of the sedimentation layer of the stationary tank were taken out by a pump.

    The oil-resistant polypropylene bag filled with the extracted oil component was filled, and nitrogen gas was sealed in the head space portion to replace oxygen. This prevents the oil from being oxidized by oxygen. The oil-resistant polypropylene bag container filled with the extracted oil was packed in a double-layer cardboard and stored with light and oxygen blocked to prevent oxidation.

  This 60 parts extracted oil is about 1% as antioxidant sesame lignans, 14.3% as saturated fatty acids of palmitic acid and stearic acid, and 84.2% as unsaturated fatty acids of oleic acid and reelic acid. A plant extract containing catalase and protease enzymes was obtained.

  The present inventor used 100% sesame essential oil (sesame oil) obtained in Example 1 for 20 people complaining of chronic persistent pruritus in hemodialysis patients (sesamesis fatty acid 15 ° C freshly squeezed omega 6). As a result, the vegetable oil containing abundant cis-type essential unsaturated fatty acids is effective for drying the skin, so that the affected area was spread once or twice a day. The increase in essential unsaturated fatty acids in the skin reduced transepidermal water loss (TEWL) and therefore caused dry skin.

  GLA content in the epidermis is high due to the enzyme required to convert linoleic acid (LA) to γ-linolenic acid (GLA), and in the case of dialysis patient's skin, the LA value is high, improving dry skin pruritus • Improvement of pigmentation was observed with itching, and most patients showed good results for reducing itching.

As in Example 2, a plant extract of sesame oil in skin disorders of dialysis patients was used.
Pruritus associated with dialysis is a frequent complication in about 60-80% of patients. In about half of them, pruritus is persistent and systemic, which leads to a decline in quality of life (QOL) due to difficulties in daily life such as discomfort, irritation, and insomnia and mental distress. The cause of pruritus is not a single one, but is one of various factors such as skin abnormalities and systemic abnormalities. This time, we tried to improve skin symptoms using the sesame oil of Example 1, which has antibacterial, antioxidant, moisturizing and relaxing effects.

The subjects were 20 hemodialysis patients (6 males and 14 females) complaining of chronic and persistent pruritus, the average age was 60.5 years, and the average dialysis history was 72.3 months.
The sesame oil of Example 1 which contains active ingredients such as vitamins and minerals and has an excellent antibacterial and antioxidative action was applied once or twice a day. Furthermore, in order to infiltrate the active ingredients, daily life guidance such as massage and avoiding skin cleanliness, avoiding irritation, and not excessively raising the skin temperature is used in combination. Etc. were observed.

  Evaluations were made in five stages using the “pruritus severity criteria table” by Akira Shiratori, asking about the degree of pruritus before and after use. Furthermore, the patient's reaction was described for each intermediate judgment, and the usability after 4 weeks was investigated.

1. Itching Score The itchiness score 4 weeks after use from the use of sesame oil is shown in FIG. The oil before and after use was 3.65 ± 0.83, and at the 4th week it was 1.05 ± 0.99, indicating a significant decrease.
2. Case presentation ・ Case 1: 70-year-old male A clear improvement in dry skin and reduction in pruritus were observed. (Fig. 4)
・ Case 2: 54 years old, female Improvement of pigmentation was observed with itching. (Fig. 5)
3. Patient response The patient's general response regarding the feeling of use of sesame oil is 15 (good for itchiness and good feeling) and 3 for slightly good (not lasting but temporary itchiness). There were two people who could not say (the grudge does not change). There were many opinions that other impressions were moist and less sticky. There was no redness of the compresses, no hot spots, no rashes, etc., and with the exception of two unchanged patients, all cases could be used continuously.
In addition, the feeling of use was slightly sticky, but there was no discontinuation due to discomfort and it is thought that continuous use is possible.

  As a result of using sesame oil, which has excellent active ingredients and moisturizing ingredients, for hemodialysis patients with dry skin, 90% of the sensation oil was found to be good and slightly good for reducing itching. Was remarkable.

  The sesame oil of sesame of Example 1 was used for 10 people, and daily massage was used as an oil massage on each person's foot for 1 week, so that the whole body was spread to about 30 mg. As a result, the situation of the representative person became as shown in FIG. 6, and each person led to mild mental stress and improved self-care awareness, and the cosmetic problem was solved.

The plant extract of Example 1 was evaluated by preparing an emulsion as follows and using it in 20 women. As a preparation method, 4 parts of sesamin oil, 1 part of beeswax, 0.1 part of royal jelly, 0.1 part of honey pollen, 0.1 part of honey and 0.1 part of procolis were heated and mixed at 80 ° C. to obtain a mixture. Further, the mixture was cooled to 30 ° C. with stirring and mixing for about 3 hours to prepare an emulsion.
Evaluation was carried out by pouring the skin of the feet of 20 women 3 times a day for 3 days.

Evaluation 1: The skin crevice was unclear, exfoliation of keratin was observed, and the skin crevice was slightly unclear. 0 evaluation 2: Although the skin groove is recognized, it is shallow or strong in one direction. Although it was mesh-like, it was slightly unclear. Evaluation by two people 3: Skin gaps were clearly recognized, and the skin was clean. 18 people As a comprehensive evaluation, by applying a poultry to women, the skin became smoother.

Manufacturing process diagram of plant extract from sesame Schematic of stone mill press Transition chart of itching score of pruritus caused by dry skin Changes in skin condition in dry skin patients (male) Changes in skin condition in patients with dry skin (female) State diagram of the skin of the foot of a woman who has been massaged for 3 days Properties of sesame seeds from Sri Lanka

Explanation of symbols

1. Motor 2. Gear 3. Squeezing stick 4. Stone mill 5. Pump 6. Sesame seeds7. Oil (plant extract)
8). Temperature sensor

10. Affected part 11. Complete cure department

Claims (5)

A plant extract comprising sesame lignans as antioxidants, an active ingredient of a saturated fatty acid, an unsaturated fatty acid, and an enzyme. The plant extract according to claim 1, which is a natural antioxidant containing sesamin, sesamorin, episesamin, sesamol, sesaminol, sesamolinol in the form of a high-concentration content containing 0.9% by weight or more of the antioxidant sesame lignans. . The total amount of saturated fatty acids palmitic acid and stearic acid is 20% by weight or less, unsaturated fatty acid oleic acid is 30% by weight or more, linoleic acid is 40% by weight or more, and the total amount of unsaturated fatty acids is 80% or more. The plant extract according to claim 1, which contains The plant extract according to claim 1, wherein the enzyme is catalase, protease, or peptidase, and contains at least one of these enzymes. The plant according to claim 1, wherein the active ingredients of the antioxidants sesame lignans, saturated fatty acids, unsaturated fatty acids and enzymes are sesame essential oils obtained by squeezing sesame seeds from the savanna zone at a low temperature of 30 ° C or lower. Extract.