JP2009534033A - 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置 - Google Patents

薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置 Download PDF

Info

Publication number
JP2009534033A
JP2009534033A JP2009506619A JP2009506619A JP2009534033A JP 2009534033 A JP2009534033 A JP 2009534033A JP 2009506619 A JP2009506619 A JP 2009506619A JP 2009506619 A JP2009506619 A JP 2009506619A JP 2009534033 A JP2009534033 A JP 2009534033A
Authority
JP
Japan
Prior art keywords
polymerase
target enzyme
dna
single molecule
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2009506619A
Other languages
English (en)
Japanese (ja)
Other versions
JP2009534033A5 (https=
Inventor
ゴエル，アニータ
Original Assignee
ナノバイオシン，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ナノバイオシン，インコーポレイテッド filed Critical ナノバイオシン，インコーポレイテッド
Publication of JP2009534033A publication Critical patent/JP2009534033A/ja
Publication of JP2009534033A5 publication Critical patent/JP2009534033A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2009506619A 2006-04-21 2007-04-23 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置 Pending JP2009534033A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79372006P 2006-04-21 2006-04-21
PCT/US2007/009747 WO2007124105A2 (en) 2006-04-21 2007-04-23 Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents

Publications (2)

Publication Number Publication Date
JP2009534033A true JP2009534033A (ja) 2009-09-24
JP2009534033A5 JP2009534033A5 (https=) 2011-06-30

Family

ID=38625640

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009506619A Pending JP2009534033A (ja) 2006-04-21 2007-04-23 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置

Country Status (6)

Country Link
US (5) US9862984B2 (https=)
EP (1) EP2016415B1 (https=)
JP (1) JP2009534033A (https=)
CN (1) CN101479605A (https=)
CA (1) CA2646309C (https=)
WO (1) WO2007124105A2 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ582558A (en) 2004-05-13 2012-06-29 Anita Goel Nano-PCR: Methods and devices for nucleic acid amplification and detection
CA2646309C (en) 2006-04-21 2016-07-26 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
EP2056849A4 (en) * 2006-08-04 2010-09-08 Univ Pennsylvania METHOD AND COMPOSITIONS FOR TREATING IGE-MEDIATED ILLNESSES
ES2387368B1 (es) * 2009-05-15 2013-08-08 Universidad De Barcelona Metodo y aparato de medida de las fuerzas opticas que actuan sobre una particula
EP2475787A2 (en) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
CN102565057B (zh) * 2011-12-15 2014-07-23 大连理工大学 一种基于左手材料的光镊
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
CN111505291B (zh) * 2020-04-14 2023-04-25 山东省千佛山医院 一种排除巨酶分子对血清酶浓度检测带来干扰的方法

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
DE4027616A1 (de) * 1990-08-31 1992-03-05 Boehringer Mannheim Gmbh Verfahren zur bestimmung von polymerase-aktivitaet
US6197508B1 (en) * 1990-09-12 2001-03-06 Affymetrix, Inc. Electrochemical denaturation and annealing of nucleic acid
US5527670A (en) * 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
AU8517391A (en) 1990-09-12 1992-03-30 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5824477A (en) * 1990-09-12 1998-10-20 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
AU690124B2 (en) * 1993-06-09 1998-04-23 Gamera Bioscience Corporation Magnetic cycle reaction
US6033850A (en) * 1994-03-15 2000-03-07 Affymetrix, Inc. Electrochemical denaturation of double-stranded nucleic acid
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
US5683875A (en) 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
WO1996041864A1 (en) 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
EP0846161B1 (en) 1995-08-25 2005-08-17 Affymetrix, Inc. (a Delaware Corporation) Release of intracellular material
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US5939291A (en) * 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9613980D0 (en) 1996-07-02 1996-09-04 Scient Generics Ltd Denaturation of double-stranded nucleic acid
GB9614544D0 (en) 1996-07-11 1996-09-04 Scient Generics Ltd Denaturation of double-stranded nucleic acid
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6074827A (en) 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
DE19710166C1 (de) 1997-03-12 1998-12-10 Bruker Franzen Analytik Gmbh Zwei-Schritt-Verfahren der DNA-Amplifikation für MALDI-TOF-Messungen
US7052650B2 (en) 1997-03-28 2006-05-30 Center National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
GB9706654D0 (en) * 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
WO1998045474A1 (en) * 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
AU7477398A (en) 1997-05-09 1998-11-27 Regents Of The University Of California, The Peltier-assisted microfabricated reaction chambers for thermal cycling
EP1003759A2 (en) 1997-08-13 2000-05-31 Cepheid Microstructures for the manipulation of fluid samples
EP1179585B1 (en) 1997-12-24 2008-07-09 Cepheid Device and method for lysis
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7601270B1 (en) * 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6929030B2 (en) * 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
IL148088A0 (en) 1999-08-13 2002-09-12 Us Genomics Inc Methods and apparatuses for stretching polymers
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6699713B2 (en) * 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES
US6793752B2 (en) * 2001-09-21 2004-09-21 The Goodyear Tire & Rubber Company Precision longitudinal registration of tire building drum to automated tire building system work station
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
US7013717B1 (en) 2001-12-06 2006-03-21 Veeco Instruments Inc. Manual control with force-feedback for probe microscopy-based force spectroscopy
WO2003061470A1 (en) 2002-01-18 2003-07-31 California Institute Of Technology Method and apparatus for nanomagnetic manipulation and sensing
MXPA04008180A (es) 2002-02-21 2005-05-16 Tokyo Gas Co Ltd Reactivo de diagnostico para evaluacion de efecto farmacologico de medicina, y metodo y reactivo para seleccionar agentes farmaceuticos que comprenden enzima, inhibidor de enzima o ligando receptor y/o promedicamentos del mismo para uno que tiene alt
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
GB2416030B (en) 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
NZ582558A (en) 2004-05-13 2012-06-29 Anita Goel Nano-PCR: Methods and devices for nucleic acid amplification and detection
CA2583498A1 (en) 2004-09-09 2006-03-16 Microfluidic Systems Inc. A handheld and portable microfluidic device to automatically prepare nucleic acids for analysis
US7315376B2 (en) 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
US7981696B2 (en) * 2005-02-18 2011-07-19 The United States of America, as represented by the Secretary of Commerce, The National Institute of Standards and Technology Microfluidic platform of arrayed switchable spin-valve elements for high-throughput sorting and manipulation of magnetic particles and biomolecules
WO2007038260A2 (en) * 2005-09-23 2007-04-05 Massachusetts Institute Of Technology Systems and methods for force-fluorescence microscopy
ES2393758T3 (es) 2006-03-15 2012-12-27 Micronics, Inc. Ensayos integrados de ácidos nucleicos
CA2646309C (en) 2006-04-21 2016-07-26 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
US7892816B2 (en) 2006-09-28 2011-02-22 Colorado State University Research Foundation Electrochemical detection of substrates
CN101558302B (zh) 2006-10-27 2013-11-06 蒙泰西托生物科学有限公司 用于改进的取样分析的便携设备
US8409877B2 (en) 2006-12-29 2013-04-02 Intel Corporation Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
US20120082985A1 (en) 2007-08-09 2012-04-05 Frederic Zenhausern Sensing And Identifying Biological Samples On Microfluidic Devices
CL2008003008A1 (es) 2007-10-12 2009-10-02 Bigtec Private Ltd Un micro dispositivo portatil de reaccion en cadena de polimerasa (pcr) basado en un micro chip de ceramica de coccion conjunta de baja temperatura (ltcc) que comprende camara de reaccion, calentador, control de temperatura del calentador, deteccion optica de interfaz de comunicacion, y el metodo para monitorearlo y controlarlo.
GB0812681D0 (en) 2008-07-10 2008-08-20 Sec Dep For Innovation Universities Apparatus and methods for effecting chemical assays
GB0818609D0 (en) 2008-10-10 2008-11-19 Univ Hull apparatus and method
DE112010002222B4 (de) 2009-06-04 2024-01-25 Leidos Innovations Technology, Inc. (n.d.Ges.d. Staates Delaware) Mehr-Proben-Mikrofluidchip fur DNA-Analyse
EP3586945A3 (en) 2009-06-05 2020-03-04 IntegenX Inc. Universal sample preparation system and use in an integrated analysis system
EP2475787A2 (en) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
US20110312537A1 (en) 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc device for amplifying and detecting target nucleic acid sequences using electrochemiluminescent resonant energy transfer, linear probes with covalently attached primers
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
HK1218096A1 (zh) 2013-03-15 2017-02-03 Nanobiosym, Inc. 用於移动设备分析核酸和蛋白质的系统和方法

Also Published As

Publication number Publication date
EP2016415A4 (en) 2009-12-16
CN101479605A (zh) 2009-07-08
US20070254279A1 (en) 2007-11-01
US11807892B2 (en) 2023-11-07
CA2646309C (en) 2016-07-26
WO2007124105A2 (en) 2007-11-01
WO2007124105A3 (en) 2008-11-27
US20250382654A1 (en) 2025-12-18
US12435351B2 (en) 2025-10-07
US9862984B2 (en) 2018-01-09
US20240309420A1 (en) 2024-09-19
US20200308618A1 (en) 2020-10-01
EP2016415B1 (en) 2013-08-14
US20180195104A1 (en) 2018-07-12
WO2007124105A4 (en) 2009-02-19
EP2016415A2 (en) 2009-01-21
CA2646309A1 (en) 2007-11-01

Similar Documents

Publication Publication Date Title
US12435351B2 (en) Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
AU773291B2 (en) UPA, a universal protein array system
US10914733B2 (en) High-throughput structure determination using nucleic acid calipers
CN109072297A (zh) 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统
CN105358709B (zh) 用于检测基因组拷贝数变化的系统和方法
JP4405371B2 (ja) 特異標的部位に結合するリガンドのスクリーニング
US20180030506A1 (en) Method for Constructing Nucleic Acid Molecule
Darcy et al. High-force application by a nanoscale DNA force spectrometer
EP2475787A2 (en) Sequence determination by use of opposing forces
JP2009534033A5 (https=)
US20240336964A1 (en) Methods and devices for detecting sars-cov-2
Kolbeck et al. HIV integrase compacts viral DNA into biphasic condensates
WO2022268932A1 (en) Systems and methods for single cell analysis
KR20230011903A (ko) Sars-cov-2를 검출하기 위한 방법 및 장치
Halvorsen et al. Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy
Qiao et al. Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules. Micromachines 2021, 12, 492
Pljevaljčić et al. Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly
Yu Development and Applications of High-Throughput Magnetic-Tweezers with Stable Temperature Control
Jia Novel Development of Super-Resolution Force Spectroscopy and Applications in Biological Systems
US20040229382A1 (en) Characterized arrangements of mixtures (CAM) using polymers
Kelso Biomedical applications of single molecule detection
Karymov et al. Effect of Single-Strand Break on Holliday Junction Migration Dynamics: A Single-Molecule Fluorescence Study

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100421

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20100421

A072 Dismissal of procedure [no reply to invitation to correct request for examination]

Free format text: JAPANESE INTERMEDIATE CODE: A073

Effective date: 20110830