JP2009297021A5 - - Google Patents

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JP2009297021A5
JP2009297021A5 JP2009115312A JP2009115312A JP2009297021A5 JP 2009297021 A5 JP2009297021 A5 JP 2009297021A5 JP 2009115312 A JP2009115312 A JP 2009115312A JP 2009115312 A JP2009115312 A JP 2009115312A JP 2009297021 A5 JP2009297021 A5 JP 2009297021A5
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dna
juvenile hormone
activity
methoprene
tolerant
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JP2009115312A
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JP2009297021A (en
JP5754681B2 (en
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Claims (22)

配列番号：42またはその1塩基を置換した配列を含み、幼若ホルモンに応答して転写を上昇させる活性またはMet（Methoprene-tolerant）蛋白質に結合する活性を有するDNA。 A DNA comprising SEQ ID NO: 42 or a sequence in which one base thereof is substituted and having an activity of increasing transcription in response to juvenile hormone or an activity of binding to a Met (Methoprene-tolerant) protein. 請求項１に記載のDNAであって、下記（ａ）〜（ｄ）のいずれかに記載のDNA。
（ａ）幼若ホルモン応答遺伝子の転写調節領域おいて、配列番号：42またはその1塩基を置換した配列を含むように切り出された配列であって、幼若ホルモンに応答して転写を上昇させる活性またはMet（Methoprene-tolerant）蛋白質に結合する活性を有する配列を含むDNA。
（ｂ）上記（ａ）のDNAにおいて、１または複数の塩基を置換、欠失、および/または付加した配列を含むDNAであって、幼若ホルモンに応答して転写を上昇させる活性またはMet（Methoprene-tolerant）蛋白質に結合する活性を有するDNA。
（ｃ）上記（ａ）のDNAの塩基配列と90％以上の同一性を有し、幼若ホルモンに応答して転写を上昇させる活性またはMet（Methoprene-tolerant）蛋白質に結合する活性を有するDNA。
（ｄ）上記（ａ）のDNAから調製したプローブとストリンジェントな条件下でハイブリダイズするDNAであって、幼若ホルモンに応答して転写を上昇させる活性またはMet（Methoprene-tolerant）蛋白質に結合する活性を有するDNA。 The DNA according to claim 1, wherein the DNA is any one of the following (a) to (d).
(A) In a transcriptional regulatory region of a juvenile hormone responsive gene, this is a sequence excised so as to include a sequence in which SEQ ID NO: 42 or one base thereof is substituted, and increases transcription in response to juvenile hormone. DNA comprising a sequence having activity or activity to bind to a Met (Methoprene-tolerant) protein.
(B) a DNA comprising a sequence obtained by substituting, deleting, and / or adding one or more bases in the DNA of (a), wherein the activity or Met ( Methoprene-tolerant) DNA that has the activity of binding to proteins.
(C) DNA having 90% or more identity with the DNA sequence of (a) above and having an activity of increasing transcription in response to juvenile hormone or binding to a Met (Methoprene-tolerant) protein .
(D) DNA that hybridizes with the probe prepared from the DNA of (a) above under stringent conditions, and binds to an activity that increases transcription in response to juvenile hormone or a Met (Methoprene-tolerant) protein DNA having the activity of 該遺伝子がKr-h1（Kruppel homolog 1）またはその相同遺伝子である、請求項１または２に記載のDNA。 The DNA according to claim 1 or 2, wherein the gene is Kr-h1 (Kruppel homolog 1) or a homologous gene thereof. 配列番号：9、10、11、12、31、34、35、36、37、38、39、または幼若ホルモン応答性を有するそれらの断片を含む、請求項１から３のいずれかに記載のDNA。 SEQ ID NO: 9, 10, 11, 12, 31, 34, 35, 36, 37, 38, 39, or a fragment thereof having juvenile hormone responsiveness, according to any of claims 1 to 3. DNA. 異種プロモーターおよび/または異種遺伝子が機能的に連結されている、請求項１から４のいずれかに記載のDNA。 The DNA according to any one of claims 1 to 4, wherein a heterologous promoter and / or a heterologous gene is operably linked. 請求項１から５のいずれかに記載のDNAを含むベクター。 A vector comprising the DNA according to any one of claims 1 to 5. 請求項６に記載のベクターを含む、幼若ホルモン応答発現用組成物。 A composition for expression of juvenile hormone response, comprising the vector according to claim 6. 請求項１から５のいずれかに記載のDNAまたは該DNAを含むベクターを含む形質転換体。 A transformant comprising the DNA according to any one of claims 1 to 5 or a vector containing the DNA. Met（Methoprene-tolerant）遺伝子が導入されている、請求項８に記載の形質転換体。 The transformant according to claim 8, wherein a Met (Methoprene-tolerant) gene is introduced. 昆虫細胞または哺乳動物細胞である、請求項８または９に記載の形質転換体。 The transformant according to claim 8 or 9, which is an insect cell or a mammalian cell. 請求項１から５のいずれかに記載のDNAを含む幼若ホルモン応答性転写エンハンサー。 A juvenile hormone responsive transcription enhancer comprising the DNA according to any one of claims 1 to 5. 請求項１から５のいずれかに記載のDNAの、幼若ホルモン応答エレメントとしての使用。 Use of the DNA according to any one of claims 1 to 5 as a juvenile hormone response element. 請求項１から５のいずれかに記載のDNAの、Met（Methoprene-tolerant）蛋白質に結合させるための使用。 Use of the DNA according to any one of claims 1 to 5 for binding to a Met (Methoprene-tolerant) protein. 幼若ホルモン、そのアゴニスト、またはアンタゴニストによる発現制御における請求項１から５のいずれかに記載のDNAの使用。 Use of the DNA according to any one of claims 1 to 5 in expression control by juvenile hormone, an agonist or antagonist thereof. 請求項１から５のいずれかに記載のDNAが導入された細胞に、幼若ホルモン、そのアゴニスト、またはアンタゴニストを接触させる工程を含む、発現制御方法。 An expression control method comprising the step of bringing a juvenile hormone, an agonist or an antagonist thereof into contact with a cell into which the DNA according to any one of claims 1 to 5 has been introduced. （ａ）請求項１から５のいずれかに記載のDNA、および（ｂ）(i)幼若ホルモン、そのアゴニスト、またはアンタゴニスト、および/または (ii) Met（Methoprene-tolerant）をコードする核酸または該核酸が導入された細胞、を含むキット。 (A) DNA according to any one of claims 1 to 5, and (b) (i) juvenile hormone, an agonist or antagonist thereof, and / or (ii) a nucleic acid encoding Met (Methoprene-tolerant) or A kit comprising a cell into which the nucleic acid has been introduced. 請求項１から５のいずれかに記載のDNAの転写活性を検出する工程を含む、幼若ホルモン作用のアッセイ方法。 A method for assaying juvenile hormone action, comprising a step of detecting the transcriptional activity of the DNA according to any one of claims 1 to 5. 請求項１７に記載の方法であって、該工程が被験化合物の存在下で行われ、それにより幼若ホルモンの作用に及ぼす被験化合物の促進または抑制効果を検出する方法。 18. The method according to claim 17, wherein the step is performed in the presence of a test compound, thereby detecting a test compound's promoting or inhibiting effect on the action of juvenile hormone. 請求項１８に記載の方法であって、該方法が幼若ホルモンの作用を上昇させる化合物のスクリーニングにおいて実施され、該DNAの転写活性を上昇させる化合物を選択する工程をさらに含む方法。 19. The method according to claim 18, further comprising the step of selecting a compound that increases the transcriptional activity of the DNA, wherein the method is carried out in screening for a compound that increases the action of juvenile hormone. 請求項１８に記載の方法であって、該工程が幼若ホルモンまたはそのアゴニストのさらなる存在下で行われ、それにより幼若ホルモンの作用を低下させる被験化合物の活性を検出する方法。 19. The method according to claim 18, wherein the step is performed in the further presence of juvenile hormone or an agonist thereof, thereby detecting the activity of a test compound that reduces the action of juvenile hormone. 請求項２０に記載の方法であって、該方法が幼若ホルモンの作用を低下させる化合物のスクリーニングにおいて実施され、該DNAの転写活性を低下させる化合物を選択する工程をさらに含む方法。 21. The method of claim 20, further comprising the step of selecting a compound that reduces the transcriptional activity of the DNA, wherein the method is carried out in screening for a compound that decreases the action of juvenile hormone. 殺虫性化合物のアッセイまたはスクリーニングにおいて実施される、請求項１７から２１のいずれかに記載の方法。 22. A method according to any of claims 17 to 21, wherein the method is performed in an assay or screening for insecticidal compounds.
JP2009115312A 2008-05-14 2009-05-12 Juvenile hormone response element Active JP5754681B2 (en)

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KR20230002766A (en) * 2020-04-21 2023-01-05 구미아이 가가쿠 고교 가부시키가이샤 Nucleic acids that interact with endocrine disruptor receptors and their uses
WO2022092170A1 (en) * 2020-10-27 2022-05-05 国立大学法人 東京大学 Method for regulating growth of decapoda crustaceans
CN113150099B (en) * 2021-02-08 2022-05-31 中国农业科学院植物保护研究所 Nanmei tomato leaf miner juvenile hormone signal pathway transcription factor Kr-h1 gene and application thereof
CN115418354B (en) * 2022-08-29 2023-06-06 江南大学 Hybridoma cell strain secreting fenoxycarb monoclonal antibody and application thereof

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