JP2009274972A - Allergen deactivator and allergen-deactivating material - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an allergen deactivator by discovering a material for deactivating the allergen from a highly safe natural product, and utilizing the material, and to provide an allergen-deactivating material. <P>SOLUTION: The allergen deactivator contains an extract from tamarind and/or persimmon tannin as an active ingredient. The allergen-deactivating material contains a carrier and the extract from tamarind and/or the persimmon tannin carried by the carrier. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an allergen inactivating agent and an allergen inactivating material, and more particularly to an allergen inactivating agent and an allergen inactivating material capable of inactivating allergens that cause type I allergic diseases.

  In recent years, with changes in the living environment, allergens that cause allergic symptoms have been present in all seasons. In particular, the number of hay fever patients in early spring due to pollen such as cedar pollen and cypress pollen has been increasing year by year, reaching 10% of the population, and the majority of them are type I against cedar pollen. Indicates an allergic reaction.

  Measures against hay fever are based on avoiding contact with pollen, and methods that avoid contact with pollen by using a mask or glasses are the mainstream, but contact with pollen is completely avoided. It is difficult. Therefore, there is a need for a technique that can fundamentally eliminate pollen allergens in people's living environment.

  Various techniques for inactivating pollen allergens have been proposed as techniques that can fundamentally eliminate pollen allergens. For example, pollen allergen inactivation spray (see Patent Document 1), filter that adsorbs and inactivates pollen allergen (see Patent Document 2), maintaining pollen allergen in the presence of heat, alkali, acid or protease Have proposed a method for inactivating pollen allergens (see Patent Documents 3 and 4), a house dust treatment agent containing a soot extract (see Patent Document 5), and the like.

  In recent years, the mite breeding has been promoted in exchange for the comfort of the indoor environment. Allergic diseases with allergens belonging to the ticks are a problem. These mites are considered to contribute to type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis.

  As a countermeasure against allergic diseases in which mites are allergens, it is conceivable to eliminate mites, which are allergens, from the living environment. However, even if the ticks are exterminated, mite allergens cannot be fundamentally eliminated due to the release of powerful allergens from the mite carcasses and mite feces into the living environment. It is difficult to solve. Therefore, there is a need for a technique that fundamentally eliminates mite allergens (ticks, mite carcasses and feces) from the living environment.

Conventionally, as a technique for fundamentally eliminating mite allergens, there is a technique in which a porous adsorbent impregnated with rosemary extract is sprayed indoors and sucked with a vacuum cleaner after several hours (see Patent Document 6). Proposed.
JP 2002-128659 A JP 2000-5553 A JP 2003-180865 A JP 2004-89673 A JP 2002-128680 A JP-A-6-256128

  An object of the present invention is to find a substance that inactivates pollen allergens or mite allergens from highly safe natural products, and to provide an allergen inactivating agent and an allergen inactivating material using the substance.

  In order to solve the above-mentioned problems, first, the allergen inactivating agent of the present invention is characterized by containing an extract from tamarind and / or salmon tannin as an active ingredient. The inactivation target of the allergen inactivating agent is preferably pollen allergen and / or mite allergen.

  Secondly, the allergen inactivating material of the present invention is characterized in that it contains a carrier, an extract from tamarind and / or tannins held in the carrier. The inactivation target of the allergen inactivating material is preferably pollen allergen and / or mite allergen.

  The allergen inactivating agent and allergen inactivating material of the present invention can inactivate pollen allergens, thereby preventing, treating or improving hay fever caused by pollen allergens. Allergens can be inactivated, thereby preventing, treating or ameliorating type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis caused by mite allergens.

The present invention will be described below.
[Allergen deactivator]
The allergen inactivating agent of the present invention contains an extract from tamarind and / or tannin as an active ingredient. In addition, examples of the inactivation target of the allergen inactivating agent of the present invention include pollen allergen, mite allergen, and the like, but are not limited thereto.

  In the present invention, the “extract from tamarind” includes an extract obtained using tamarind as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product thereof. Both products and purified products are included.

  An extract from tamarind which is an active ingredient of the allergen inactivating agent of the present invention can be obtained by an extraction treatment using tamarind (scientific name: Tamarindus indica L.) which is a plant as an extraction raw material.

  Tamarindus indica L. is a semi-evergreen tree belonging to the genus Tamarindus, which is native to tropical Africa and widely cultivated in the tropics, and can be easily obtained from these regions. Examples of the constituent parts of tamarind that can be used as an extraction raw material include leaf parts, trunk parts, bark parts, branch parts, fruit parts, seed parts, seed coat parts, root parts, etc., preferably seed coat parts (also known as tamarind). Husque). Tamarind husk is a polysaccharide obtained from the endosperm portion of tamarind seeds, and is a hard seed coat that is discarded when producing tamarind gum widely used in the food field as a thickener. It is used as a raw material for tannin production.

  The details of the allergen-inactivating substance contained in the extract from tamarind are unknown, but an extract having an allergen-inactivating action is obtained from tamarind by an extraction method generally used for plant extraction. be able to.

  For example, an extract having an allergen inactivating effect can be obtained by drying a plant as an extraction raw material, and then pulverizing the plant as it is or using a pulverizer and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction with a polar solvent can be performed efficiently.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by volume of a lower aliphatic alcohol with respect to 10 parts by volume of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by volume of a lower aliphatic ketone with 10 parts by volume of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 1 to 90 parts by volume of a polyhydric alcohol with respect to the volume part.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent in an amount 5 to 15 times (mass ratio) of the extraction raw material, the soluble component is extracted at room temperature or under reflux heating, and then filtered to remove the extraction residue. Can be obtained. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of an allergen inactivating agent, but a concentrated solution or a dried product is easier to use.

  The persimmon tannin which is an active ingredient of the allergen inactivating agent of the present invention can be obtained by subjecting persimmon astringent to purification treatment. In addition, since persimmon astringent containing persimmon tannin also has an allergen inactivating action, it can be used as an active ingredient of the allergen inactivating agent of the present invention, but by subjecting persimmon astringent to purification treatment to increase the purity of persimmon tannin The allergen inactivating action of the allergen inactivating agent can be further improved.

  柿 Shibu is a squeezed fruit of the persimmon (Diospyros kaki L.) belonging to the genus Oysteraceae, a deciduous tree that is widely distributed in Honshu, Shikoku, Kyushu, etc. Although it can obtain as a liquid or squeezed juice, what is obtained by pressing or squeezing the fruit of astringent persimmon with much content of persimmon astringent is preferable. The persimmon astringent subjected to the refining treatment may be a pressed liquid or a squeezed liquid, or a roughly purified product thereof.

  In refining persimmon astringent, first, persimmon astringent and a synthetic polymer adsorbing resin are brought into contact with each other, and persimmon tannin contained in persimmon astringent is adsorbed on the synthetic polymer adsorbing resin.

  The synthetic polymer adsorbing resin is preferably a nonpolar or intermediate polar resin, such as Diaion HP-10, Diaion HP-20, Diaion HP-30, Diaion HP-40, or Diaion HP. Styrene / divinylbenzene series such as -50 (all manufactured by Mitsubishi Kasei Co., Ltd.), Amberlite XAD-2, Amberlite XAD-4 (all manufactured by Rohm and Haas), Lebatit OC-1031 (manufactured by Bayer) Examples thereof include those having a copolymer as a resin matrix; or those having a polyacrylic acid ester such as Amberlite XAD-7 and Amberlite XAD-8 (both manufactured by Rohm and Haas) as the resin matrix.

  The amount of resin to be brought into contact with the persimmon astringent is preferably 2 to 20 times (by mass) the dry solid content of the persimmon. If the amount of resin is less than twice the amount, tannin may leak without being sufficiently adsorbed to the resin, and if the amount of resin exceeds 20 times the amount of impurities adsorbed increases, purity There is a risk that it will be difficult to obtain high-grade tannins. For this reason, by setting the amount of resin within the above range, it is possible to efficiently collect persimmon tannin contained in persimmon astringency.

  The method for bringing the persimmon astringent and the resin into contact is not particularly limited, and examples thereof include a batch method and a column method, but the column method is preferable from the viewpoint of operability and processing efficiency.

  Next, soot tannin is eluted from the resin adsorbed with soot tannin. The method for eluting soot tannin from the resin is not particularly limited. For example, water and alcohol are passed in that order through a column packed with resin adsorbing soot tannin to elute soot tannin. Let Thereby, a persimmon tannin is obtained as an alcohol fraction.

  Examples of the alcohol that can be used as the eluent include, but are not limited to, lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol, or aqueous solutions thereof. is not. When an aqueous solution of a lower aliphatic alcohol is used as the eluent, the water content is preferably 50% by volume or less, and more preferably 30% by volume or less. If the water content of the eluate exceeds 50% by volume, the yield of soot tannin may be reduced.

  Furthermore, the low molecular weight contained in the alcohol fraction is obtained by bringing the alcohol fraction obtained as described above into contact with activated carbon having a solid content of 10 to 100% under a temperature condition of 10 to 30 ° C. for 1 to 10 hours. Impurities such as polyphenol can be adsorbed and removed to obtain a decolorizing solution containing tannin. The concentrated decolorizing solution thus obtained is concentrated, and if necessary, spray-dried, freeze-dried, or the like, whereby a purified liquid or powdered purified koji tannin can be obtained.

  Since the extract from tamarind or salmon tannin obtained as described above has an allergen inactivating action, it can be used as an active ingredient of an allergen inactivating agent by utilizing the action.

  The allergen inactivating agent of the present invention may consist only of an extract from tamarind and / or salmon tannin, or may be an extract of tamarind and / or salmon tannin. Good.

  The extract from tamarind and / or salmon tannin is in the form of powder, granule, tablet, liquid, etc. according to a conventional method using a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and any other auxiliary agent. It can be formulated into any dosage form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring agent and the like can be used. Extracts from tamarind and / or salmon tannin can be used by blending with other compositions (cosmetic compositions, etc.), and also used as ointments, solutions for external use, eye drops, nasal drops, etc. You can also.

  The allergen inactivating agent of the present invention may contain any one of an extract from tamarind and koji tannin as an active ingredient, or may contain these in combination as an active ingredient. When these are combined and contained as an active ingredient in the allergen inactivating agent, the compounding ratio is not particularly limited, and can be appropriately adjusted according to the strength of the physiological activity.

  The concentration of the extract from tamarind or the tannin concentration in the allergen inactivating agent of the present invention is not particularly limited, but is preferably 0.001 to 0.1% by mass. In particular, when the inactivation target in the allergen inactivating agent of the present invention is a pollen allergen, a high concentration of 0.01 to 0.1% by mass, particularly 0.1% by mass or more, as shown in Examples described later If so, a more excellent pollen allergen inactivating effect can be exhibited, and a sufficient pollen allergen inactivating effect can be exhibited even at a low concentration of 0.001 to 0.01% by mass. . In addition, when the inactivation target in the allergen inactivating agent of the present invention is mite allergen, it is more preferably 0.01% by mass or more, as shown in Examples described later, and 0.1% by mass. % Is particularly preferred, but a sufficient mite allergen inactivating action can be exhibited even at a low concentration (about 0.001% by mass).

  The allergen inactivating agent of the present invention inactivates the pollen allergen that causes type I allergic disease through the pollen allergen inactivating action of the extract from tamarind and / or tannin, and pollen caused by the pollen allergen Can be prevented, treated or ameliorated, and the mite allergen that causes type I allergic disease is inactivated through the mite allergen inactivating action of the extract from tamarind and / or sputum tannin. Type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis that are caused can be prevented or improved.

  The pollen allergen is not particularly limited as long as it is a pollen having an action of causing type I allergic diseases, and examples thereof include cedar pollen, cypress pollen, ragweed pollen, camodia pollen, etc. The activator is particularly effective against cedar pollen.

  The mite allergen is not particularly limited as long as it is a mite having an action causing type I allergic disease. For example, the mite allergen belongs to the genus Lepidoptera, Dermatophagoides pteronyssinus, such as Dermatophagoides farinae Examples include ticks, feces or dead bodies of these ticks.

  Allergen inactivation means that allergens (pollen allergen, mite allergen, etc.) lose the action of causing allergic symptoms. The application target of the allergen inactivating agent of the present invention is, for example, hay fever causing allergic symptoms by pollen allergens, type I allergic diseases causing allergic symptoms by mite allergens, etc. Examples include rhinitis, sneezing, asthma and conjunctivitis.

  In addition, although the allergen inactivating agent of the present invention is suitably applied to humans, it can also be applied to animals other than humans as long as their effects are exhibited.

[Allergen inactivating material]
The allergen inactivating material of the present invention contains a carrier and an extract from tamarind and / or salmon tannin retained in the carrier. In the allergen-inactivating material of the present invention, an extract from tamarind and / or an allergen-inactivating agent formulated with tannins may be held in a carrier.

  As the extract from tamarind and / or salmon tannin held in the carrier, the same extract as tamarind and / or salmon tannin contained as an active ingredient in the allergen inactivating agent described above may be used. .

  The carrier is not particularly limited as long as it can retain the extract from tamarind and / or tannin on the surface, and may be a porous body or a non-porous body. However, it is preferable that the porous body has a large surface area capable of holding a larger amount of an extract from tamarind and / or tannin.

  Examples of the porous body include fiber assemblies such as knitted fabrics, woven fabrics, nonwoven fabrics, and papers, porous resin molded bodies such as urethane foam, activated carbon, and porous ceramic materials. Examples of the non-porous body include a non-porous resin molded body, a metal material, a ceramic material, and a stone material.

  The shape of the carrier is not particularly limited, and may be, for example, a sheet shape or a granular shape. Furthermore, filters such as air conditioner filters, air purifier filters, ventilation device filters, masks, clothes, furniture, walls (wall materials), wallpaper, posters, seals, and the like can be used as carriers.

  The extract from tamarind and / or salmon tannin may be held on the surface of the carrier so that these extracts and allergens scattered in the air can come into contact with each other. There are no particular limitations on the method used for holding. Here, the surface of the carrier means a part of the carrier where the carrier and the allergen can come into contact, and the surface of the carrier is not limited to the outer surface of the carrier but is a part existing inside the carrier and is in contact with the allergen. Including possible sites.

  Examples of a method for holding the extract from tamarind and / or tannin in the carrier include, for example, (1) a method in which the carrier is impregnated with an extract from tamarind and / or an aqueous solution of tannin and dried. (2) A method of blending an extract from tamarind and / or tannin in the carrier material in the production process of the carrier so that an extract from tamarind and / or tannin appears on the surface of the carrier, (3) There are a method of spraying an extract of tamarind and / or an aqueous solution of tannin on the surface of the carrier, (4) a method of coating an extract of tamarind and / or tannin on the surface of the carrier via a binder, etc. Can be mentioned.

  When the carrier is impregnated with an extract of tamarind and / or an aqueous solution of salmon tannin, the impregnation time is not particularly limited as long as the carrier can retain the extract of tamarind and / or salmon tannin. And usually 0.1 to 3 hours, preferably 0.5 to 2 hours.

The amount of the extract from tamarind and / or salmon tannin held in the carrier is particularly an amount that can inactivate the allergen by contacting the allergen with the extract from tamarind and / or salmon tannin. but are not limited to, usually 0.01 to 10 g / ^{m 2,} preferably 0.1-5 g / ^{m 2.}

  The allergen inactivating material described above can be used as filters such as air conditioner filters, air purifier filters, and ventilator filters, masks, clothes, and the like. If the allergen inactivating material is used as a filter, it is possible to prevent the allergen from re-scattering while having allergic activity. If an allergen inactivating material is used as a mask, fine allergens that pass through the mask can be inactivated. Furthermore, if an allergen inactivating material is used as clothing, it is possible to prevent the allergen adhering to the clothing from entering the room or the like while having allergic activity.

  When the allergen-inactivating material of the present invention is held in the air, the pollen allergen scattered in the air contacts with the extract from tamarind and / or tannin held in the carrier, and the extraction from tamarind The pollen allergen inactivation of the product and / or tannin can inactivate the pollen allergen, and the mite allergen inactivation of the extract from tamarind and / or tannin Can be inactivated.

  Therefore, according to the allergen inactivating material of the present invention, it is possible to prevent, treat or ameliorate hay fever, which is a type I allergic disease caused by pollen allergens, as well as Dermatophagoides farinae, Dermatophagoides pteronyssinus ) Prevents, treats or improves type I allergic diseases such as allergic asthma, rhinitis, conjunctivitis, and atopic dermatitis caused by mite belonging to the genus Lepidoptera, Mite, and mite allergens such as feces and carcasses of these mites can do.

  Hereinafter, although a manufacture example and a test example are shown and this invention is demonstrated concretely, this invention is not restrict | limited at all to the following manufacture example and test example. In this test example, tamarind 50% by volume ethanol extract (manufactured by Maruzen Pharmaceutical Co., Ltd., trade name: Tamarind Husk, Example 1) and salmon tannin produced in Production Example 1 below (Example 2) were used as samples. ), Green tea extract (manufactured by Maruzen Pharmaceutical Co., Ltd., trade name: green tea extract MF, Comparative Example 1), Keihi extract (manufactured by Maruzen Pharmaceutical Co., Ltd., trade name: Keihi extract W-LA, Comparative Example 2), tannic acid (Wako Pure Chemical Industries, trade name: tannic acid, Comparative Example 3), gallic acid hydrate (Tokyo Chemical Industry Co., Ltd., trade name: gallic acid monohydrate, Comparative Example 4) and mimosa tannin (Mimosa) Product name: Mimosa ME powder, Comparative Example 5) manufactured by Central Co., Ltd. was used.

[Production Example 1] Production of persimmon tannin A space velocity SV = 0.5 / in a column packed with 400 g of Diaion HP-20 (manufactured by Mitsubishi Kasei) with 1 L (solid content: 10%) of pressed fruit of astringent fruit The solution was passed through hr. Thereafter, 2 L of water was passed through the column, and then 2 L of 70 vol% ethanol was passed at a space velocity of SV = 2 / hr to elute soot tannin. The obtained eluate was brought into contact with activated carbon at 25 ° C. for 5 hours, and then concentrated and dried to obtain 25.6 g of soot tannin powder (Example 2).

[Test Example 1] Cedar pollen allergen inactivation test Each sample (Examples 1 and 2 and Comparative Examples 1 to 5) was tested for its inactivation effect on cedar pollen allergens as follows.

(1) Cedar pollen allergen inactivation reaction Each sample (Examples 1 and 2 and Comparative Examples 1 to 5) was dissolved in a PBS solution containing 10% DMSO at the concentrations shown in Table 1 to prepare each sample solution. did. 100 μL of each sample solution was added to each 96-well microplate, and cedar pollen allergen (product name: purified cedar pollen antigen Cry j 1, manufactured by Seikagaku Corporation) was dissolved in 0.1% bovine serum albumin-containing PBS solution. A 2 ng / mL cedar pollen allergen solution was added at 100 μL per well and shaken at room temperature for 2 hours. In addition, as a control, a solution obtained by adding a cedar pollen allergen solution to a PBS solution containing 10% DMSO to which no sample was added was similarly shaken.

  After shaking, 100 μL of cedar pollen allergen solution was collected from each hole of the microplate, and the concentration of cedar pollen allergen (ng / mL) present in the solution was measured by sandwich ELISA as shown below.

(2) Quantification of cedar pollen allergen concentration (sandwich ELISA method)
100 μL of a 10 μg / mL coating solution (product name: anti-Cry j 1 monoclonal antibody 013, manufactured by Seikagaku Corporation) was added to each hole of the ELISA plate and allowed to stand at room temperature for 2 hours. Thereafter, the coating solution was removed, 250 μL of 0.1% bovine serum albumin-containing PBS solution was added, and the mixture was allowed to stand at 4 ° C. overnight. Thereafter, the PBS solution containing 0.1% bovine serum albumin was removed, 100 μL of the cedar pollen allergen solution shaken for 2 hours was added, and the mixture was shaken at room temperature for 2 hours.

  As a standard, cedar pollen allergen (product name: purified cedar pollen antigen Cry j 1, manufactured by Seikagaku Corporation) was dissolved in a PBS solution containing 0.1% bovine serum albumin, 4 ng / mL, 2 ng / mL, 1 ng. Standard solutions for calibration curves of / mL, 0.5 ng / mL, and 0.25 ng / mL were prepared. 100 μL of standard solution for calibration curve at each concentration was added to the ELISA plate and shaken at room temperature for 2 hours. After shaking, the ELISA plate was washed three times with 300 μL of a PBS solution containing 0.05% Tween 20, and then diluted 1000 times to a cedar pollen allergen monoclonal antibody (product name: horseradish peroxidase-labeled anti-Cry j 1 monoclonal antibody 053). 100 μL of Seikagaku Corporation) was added and shaken at room temperature for 2 hours. The ELISA plate was washed 3 times with 300 μL of PBS solution containing 0.05% Tween 20 and then 120 μL of 0.3 mg / mL ABTS solution was added to develop color at room temperature, reacted for 10 to 20 minutes, and then mixed. The absorbance at 405 nm was measured with a microplate reader.

  Using the calibration curve obtained from the absorbance of the standard solution for the calibration curve, the cedar pollen allergen concentration in the sample-added cedar pollen allergen solution and the cedar pollen allergen concentration in the sample-free cedar pollen allergen solution were quantified. The cedar pollen allergen inactivation rate (%) was calculated based on the following formula using the quantitative results.

Cedar pollen allergen inactivation rate (%) = (B−A) / B × 100
In the formula, A represents “the cedar pollen allergen concentration in the sample-added cedar pollen allergen solution (ng / mL)”, and B represents “the cedar pollen allergen concentration in the sample-free cedar pollen allergen solution (ng / mL)”. To express.
The results of the above test are shown in Table 1.

  As shown in Table 1, it was confirmed that the tamarind extract of Example 1 and the persimmon tannin of Example 2 have excellent cedar pollen allergen inactivating action, and particularly high concentration (0.01% by mass or more, It was confirmed that the cedar pollen allergen inactivating effect was preferably 0.1% by mass or more. On the other hand, the hydrolyzable tannins of Comparative Examples 1 to 4 showed almost no cedar pollen allergen inactivating action. Therefore, tamarind extract (Example 1) and salmon tannin (Example 2) are compared to hydrolyzed tannins (Comparative Examples 1 to 4) known to have an allergen inactivating action. It is considered that the cedar pollen allergen inactivation action is significant.

  Moreover, it was confirmed that the tamarind extract of Example 1 and the persimmon tannin of Example 2 have the cedar pollen allergen inactivation effect superior to the condensed tannin (Mimosatannin) of Comparative Example 5. From this, the tamarind extract (Example 1) and persimmon tannin (Example 2) are excellent cedar pollen allergens, although they are generally condensed tannins known to have a weak allergen inactivating effect. It is thought to have an inactivating action.

[Test Example 2] Mite allergen inactivation test The mite allergen inactivation action of each sample (Examples 1-2 and Comparative Examples 1-5) was tested as follows.

  50 μL of a 2000 ng / mL coating solution (product name: anti-Der fII monoclonal antibody 15E11, manufactured by Seikagaku Corporation) was added to each well of the ELISA plate and allowed to stand at 4 ° C. overnight. The ELISA plate was washed three times with 300 μL of a PBS solution containing 0.05% Tween 20, and then 200 μL of a 1% bovine serum albumin-containing PBS solution was added and allowed to stand at room temperature for 1 hour for blocking. After blocking, the plate was washed three times with 300 μL of PBS solution containing 0.05% Tween 20, and mite allergen inactivation reaction was performed as shown below.

(1) Mite allergen inactivation reaction Each sample (Examples 1 and 2 and Comparative Examples 1 to 5) is dissolved in a PBS solution containing 10% DMSO so as to have the concentrations shown in Table 2, and each sample solution Was prepared. After completion of blocking, each sample solution was added to the ELISA plate after washing at 25 μL per well, and mite allergen (product name: purified mite antigen Der fII, manufactured by Seikagaku Corporation) was dissolved in PBS solution at 200 ng / 25 mL of mite allergen solution was added per well and shaken at room temperature for 2 hours. As a control, a PBS solution containing 10% DMSO was added to an ELISA plate, and then a mite allergen solution was added and shaken in the same manner.

  Also, as a standard, mite allergen is dissolved in a PBS solution to prepare standard solutions for calibration curves of 300 ng / mL, 150 ng / mL, 75 ng / mL, 37.5 ng / mL, 18.8 ng / mL, 9.4 ng / mL. did. 50 μL of standard solution for calibration curve at each concentration was added to the ELISA plate and similarly shaken at room temperature for 2 hours.

(2) Determination of mite allergen concentration (sandwich ELISA method)
After shaking, the ELISA plate was washed 3 times with 300 μL of PBS solution containing 0.05% Tween 20, and then diluted 2000 times. Mite allergen monoclonal antibody (product name: horseradish peroxidase labeled anti-Der fII monoclonal antibody 13A4PO, biochemistry (Manufactured by Kogyo) was added and shaken at room temperature for 2 hours. Then, the ELISA plate was washed three times with 300 μL of PBS solution containing 0.05% Tween 20, and then 100 μL of 0.3 mg / mL ABTS solution was added to cause color development at room temperature, followed by mixing for 10 to 20 minutes, Absorbance at 405 nm was measured with a microplate reader.

  Using the calibration curve obtained from the absorbance of the standard curve standard solution, the mite allergen concentration in the sample-added mite allergen solution and the mite allergen concentration in the sample-free mite allergen solution were quantified. The mite allergen inactivation rate (%) was calculated based on the following formula using the quantitative result.

Mite allergen inactivation rate (%) = (B−A) / B × 100
In the formula, A represents “the mite allergen concentration in the sample-added mite allergen solution (ng / mL)”, and B represents “the mite allergen concentration in the sample-free mite allergen solution (ng / mL)”.
The results of the above test are shown in Table 2.

  As shown in Table 2, the tamarind extract of Example 1 and the persimmon tannin of Example 2 have a mite allergen inactivating effect superior to the hydrolyzable tannins of Comparative Examples 1 to 4, In particular, it was confirmed that even a low concentration (0.001% by mass) has an excellent mite allergen inactivating action.

  In addition, it was confirmed that the tamarind extract of Example 1 and the salmon tannin of Example 2 have an excellent mite allergen inactivating action as compared with the condensed tannin (Mimosatannin) of Comparative Example 5. From this, it can be considered that the tamarind extract (Example 1) and strawberry tannin (Example 2) have particularly excellent mite allergen inactivating action among the condensed tannins.

  The allergen inactivating agent and the allergen inactivating material of the present invention are hay fever caused by pollen allergen, type I such as allergic asthma, rhinitis, conjunctivitis, atopic dermatitis caused by mite, mite carcasses and feces mite allergen It is useful for prevention, treatment or improvement of allergic diseases.

Claims (4)

  An allergen inactivating agent comprising an extract from tamarind and / or tannin as an active ingredient.   The allergen inactivating agent according to claim 1, wherein the allergen is a pollen allergen and / or a mite allergen. A carrier;
An allergen-inactivating material comprising an extract from tamarind and / or salmon tannin held in the carrier.   The allergen inactivating material according to claim 3, wherein the allergen is a pollen allergen and / or a mite allergen.