JP2009264787A - Optical image measuring device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an optical image measuring device, capable of also acquiring other types of images in an object to be measured, in addition to the OCT image of the object to be measured. <P>SOLUTION: The optical image measuring device 100 functions as a full-field type OCT device. Furthermore, the optical image measuring device 100 can acquire the spectral image of the object 1000 to be measured. For that purpose, a halogen lamp 1 and a filter 2b output photographic light, including a prescribed wavelength component. A drive mechanism 19 shuts off a reference optical path, by retracting a reflection mirror 4 from the reference optical path. A drive mechanism 26 retracts a polarization beam splitter 14 from an interference optical path, and arranges an optical path length correction member 15 in the interference optical path. The photographic light is applied to the object 1000 to be measured via a signal optical path, and is guided via the signal optical path and interference optical path. A CCD 18 detects the guided photographic light and forms the spectral image of the object 1000 to be measured. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention projects the signal light having a predetermined beam diameter onto the object to be measured, and detects the interference light between the reflected light or transmitted light and the reference light, thereby representing the surface aspect and the internal aspect of the object to be measured. The present invention relates to an optical image measurement device that forms an image.

  2. Description of the Related Art In recent years, an optical image measurement technique that forms an image of the surface or inside of an object to be measured using light attracts attention. Since the optical image measurement technique does not have invasiveness to the human body unlike conventional X-ray CT, it is expected to be applied particularly in the medical field.

  As a typical technique of optical image measurement technology, there is a technique called optical coherence tomography (OCT). For example, in Patent Document 1, a light beam is divided into signal light and reference light, and the interference light is generated and detected by superimposing the signal light passing through the object to be measured and the reference light passing through the reference object. Discloses a method of forming an image representing the surface of the object to be measured and the internal aspect thereof. An image acquired by applying the OCT technique may be referred to as an OCT image.

  Here, the signal light projected on the object to be measured has a predetermined beam diameter. Thereby, an image of a cross section substantially orthogonal to the traveling direction of the signal light is formed. Such an optical coherence tomographic imaging method is called a full-field type or an en-face type.

  The full-field type optical image measurement device is equipped with an interferometer and has a feature that an image with high magnification and high resolution can be acquired. For example, when applied to the medical field or the biological field, it is possible to acquire an image representing a cell form.

  By the way, in the medical field or the like, a plurality of types of images may be referred to in order to inspect an object to be measured from various angles. Assuming such a case, an apparatus capable of acquiring a plurality of types of images has been proposed. For example, the fundus oculi observation device described in Patent Document 2 has a function as an optical image measurement device and a function as a fundus camera.

  The fundus oculi observation device of Patent Document 2 is equipped with a spectral-domain type optical image measurement device that forms an image based on the spectral distribution of interference light. Spectral domain types include a Fourier-domain type that spectrally decomposes interference light, and a swept-source type that uses a light source that can output light of various wavelengths.

  In addition to general color photography, there are spectroscopic photography, fluorescence photography, and the like as a technique for photographing the object to be measured (see, for example, Patent Documents 3 and 4). Spectral imaging is a technique for capturing images at various depths by using the fact that the depth of light varies depending on the wavelength. Fluorescence imaging is a technique for imaging a distribution state of a fluorescent agent by applying a fluorescent agent to an object to be measured and using light having an excitation wavelength of the fluorescent agent. Fluorescence imaging is used when observing the leakage state of blood from blood vessels. For example, in the ophthalmic field, there are fluorescein fluorescence photography and indocyanine green fluorescence photography.

  There is also a technique called autofluorescence imaging (autofluorescence imaging) in which the distribution state of the target substance is imaged by using light of the excitation wavelength of the target substance without using a fluorescent agent (see, for example, Patent Document 4) ). Autofluorescence imaging is used, for example, for observing a substance (lipofustin) that accumulates in a living body due to aging, which is noted as an early symptom of age-related macular degeneration, which is an eye disease.

  In the fields of biochemistry and medicine, a site or substance of interest is identified by observing with a fluorescence microscope a cell containing a fluorescent substance as a marker.

JP 2007-212467 A JP 2007-275374 A JP 2006-61328 A Special table 2007-521920 gazette

  As described above, there are apparatuses that can acquire a plurality of types of images. However, conventional full-field optical image measurement apparatuses cannot acquire images other than OCT images.

  The present invention has been made to solve the above problems, and an optical image measurement device capable of acquiring not only an OCT image of an object to be measured but also other types of images of the object to be measured. The purpose is to provide.

  In order to achieve the above object, the invention according to claim 1 divides broadband light having a predetermined beam diameter into signal light and reference light, and passes the signal light through the object to be measured through the signal light path. And the reference light passing through the reference object through the reference light path are generated to generate interference light, and the interference light guided through the interference light path is detected to form an image of the object to be measured An optical image measurement device, wherein a blocking unit that blocks the reference optical path, an output unit that outputs photographing light in a state where the reference optical path is blocked, and a signal light path for photographing light output from the output unit An optical system that irradiates the object to be measured through the optical system and guides the imaging light passing through the object to be measured through the signal optical path and the interference optical path, and detects the imaging light through the optical system to detect an image. And image forming means for forming And wherein the door.

  The invention according to claim 2 is the optical image measurement device according to claim 1, wherein the output means outputs the photographing light including a predetermined wavelength component, and the image forming means An image is formed by detecting the predetermined wavelength component via the optical system.

  The invention according to claim 3 is the optical image measurement device according to claim 2, wherein the output means includes a light source that generates the broadband light and the predetermined wavelength of the generated broadband light. A filter that transmits a component, and the optical system irradiates the object to be measured with the wavelength component transmitted through the filter as the photographing light.

  According to a fourth aspect of the present invention, there is provided the optical image measurement device according to the third aspect, wherein the output means includes a plurality of the filters that transmit different wavelength components, and a broadband generated by the light source. Control means for selectively arranging the plurality of filters in an optical path of light.

  The invention according to claim 5 is the optical image measurement device according to claim 2, wherein the output means is a wavelength tunable light source capable of generating a plurality of imaging lights having different wavelengths, and the wavelength tunable light source. And control means for sequentially generating a plurality of imaging light beams having different wavelengths.

  The invention according to claim 6 is the optical image measurement device according to claim 2, wherein the output means is selected from a plurality of light sources that output photographing light of different wavelengths and the plurality of light sources. And a control means for outputting photographing light.

  The invention according to claim 7 is the optical image measurement device according to claim 1, wherein the output means includes a light source that generates the broadband light as the photographing light, and the optical system includes the optical system, Including extraction means for extracting a predetermined wavelength component from the broadband light passing through the object to be measured, wherein the image forming means detects the extracted predetermined wavelength component to form an image. .

  The invention according to claim 8 is the optical image measurement device according to claim 7, wherein the extraction means includes a filter that transmits a predetermined wavelength component of the broadband light that has passed through the object to be measured. And the image forming means detects the predetermined wavelength component transmitted through the filter to form an image.

  The invention according to claim 9 is the optical image measurement device according to claim 8, wherein the extraction means passes through the plurality of filters that transmit different wavelength components and the object to be measured. Control means for selectively arranging the plurality of filters in the optical path of the broadband light.

  The invention according to claim 10 is the optical image measurement device according to claim 7, wherein the extraction means disperses the broadband light passing through the object to be measured into a plurality of wavelength components. The image forming means forms any of the plurality of wavelength components to form an image.

  The invention according to claim 11 is the optical image measurement device according to claim 1, wherein the output means outputs photographing light including an excitation wavelength component of a fluorescent substance in the object to be measured, The optical system irradiates the object to be measured with imaging light including the excitation wavelength component, and guides fluorescence emitted from the fluorescent material upon receiving the imaging light through the signal optical path and the interference optical path. The image forming means detects fluorescence passing through the optical system and forms a distribution image of the fluorescent material on the object to be measured.

  The invention according to claim 12 is the optical image measurement device according to claim 11, wherein the output means includes a light source that generates the broadband light and the excitation wavelength component of the generated broadband light. An exciter filter that transmits light, the optical system irradiates the object to be measured with the excitation wavelength component that has passed through the filter as the imaging light, and the optical system is a barrier that blocks wavelength components other than the fluorescence. The image forming means includes a filter, and detects the fluorescence transmitted through the barrier filter to form the distribution image.

  The invention according to claim 13 is the optical image measurement device according to claim 11, wherein the output means includes a light source that generates the broadband light as the photographing light, and the optical system includes the optical system, The object to be measured is irradiated with broadband light generated by a light source, the optical system includes a barrier filter that blocks wavelength components other than the fluorescence, and the image forming means detects fluorescence that has passed through the barrier filter. Then, the distribution image is formed.

  The invention according to claim 14 is the optical image measurement device according to claim 1, wherein the optical system includes ring slits in both the signal light path and the interference light path, and the image forming means. Is characterized in that it detects photographic light passing through the optical system including both ring slits to form a phase difference image.

  The invention according to claim 15 is the optical image measurement device according to any one of claims 1 to 14, wherein the broadband light is divided into the signal light and the reference light. A reference mirror as the reference object, and the reference light divided from the broadband light by the dividing member toward the reference mirror, and the reference reflected by the reference mirror. A reflecting mirror that reflects light toward the dividing member is provided, and the blocking means includes a drive mechanism that moves the reflecting mirror.

  The invention described in claim 16 is the optical image measurement device according to any one of claims 1 to 14, wherein the blocking means includes a shutter that blocks the reference light. It is characterized by.

  The invention according to claim 17 is the optical image measurement device according to claim 16, wherein the shutter is provided obliquely with respect to the traveling direction of the reference light.

  The optical image measurement device according to the present invention divides broadband light having a predetermined beam diameter into signal light and reference light, and causes signal light passing through the object to be measured and reference light passing through the reference object to interfere with each other. It has a function of generating interference light and detecting the interference light to form an image (OCT image) of the object to be measured.

  Furthermore, the optical image measurement device according to the present invention outputs imaging light in a state where the reference optical path is blocked, irradiates the object to be measured with the imaging light through the signal optical path, and uses the imaging light via the object to be measured. Detection can be performed by guiding light through the signal light path and the interference light path, and an image can be formed based on the detection result.

  According to such an optical image measurement device, in addition to the OCT image of the object to be measured, other types of images of the object to be measured can be acquired. Examples of the other types of images include a spectral image, a fluorescence image, and a phase difference image.

  An example of an embodiment of an optical image measurement device according to the present invention will be described in detail with reference to the drawings.

  Hereinafter, two embodiments will be mainly described. In the first embodiment, an apparatus capable of acquiring an OCT image and a spectral image will be described. Here, the spectral image means an image obtained by spectral imaging. In the second embodiment, an apparatus capable of acquiring an OCT image and a fluorescence image will be described. Here, the fluorescence image means an image obtained by fluorescence imaging.

  Although not described in detail, the optical image measurement device according to the present invention may be configured to be able to acquire a type of image other than a spectral image or a fluorescence image. As an example, the optical image measurement device according to the present invention may be configured to be able to acquire a phase contrast image by a phase contrast microscope, a differential interference image by a differential interference microscope, and the like together with an OCT image.

<First Embodiment>
[Constitution]
The optical image measurement device according to this embodiment is a device that can acquire an OCT image and a spectral image. A configuration example of the optical image measurement device according to this embodiment is shown in FIG. The optical image measurement apparatus 100 functions as a full field type OCT apparatus.

  The object to be measured 1000 is arranged in a state suitable for measurement. For example, when the object to be measured 1000 is a living eye, jelly, liquid, or the like for reducing the change in the refractive index at the boundary can be applied to the object to be measured 1000. When the object to be measured 1000 is an extracted eye, the object to be measured 1000 can be placed in a liquid immersion state in order to reduce the change in the refractive index at the boundary. When the object to be measured 1000 is a cultured cell, it is possible to arrange the cultured cell in a petri dish in a liquid immersion state.

  The optical image measuring device 100 includes a halogen lamp 1. The halogen lamp 1 outputs, for example, non-polarized broadband light M. In addition, although illustration is abbreviate | omitted, the halogen lamp 1 contains the optical fiber bundle which guides output light with a normal halogen lamp, the Koehler illumination optical system for illuminating the irradiation field of output light uniformly, etc. Can be configured. The broadband light M output from the halogen lamp 1 has a predetermined beam diameter.

  The light source for acquiring the OCT image is not limited to the halogen lamp 1 and may be any light source that outputs non-polarized broadband light. For example, an arbitrary thermal light source (a light source based on black body radiation) such as a xenon lamp can be applied. The light source may be a laser light source that outputs broadband light with random polarization. Here, non-polarized light means a polarization state including linearly polarized light, circularly polarized light, and elliptically polarized light. Random polarization means a polarization state having two linearly polarized light components orthogonal to each other and the power of each linearly polarized light component changes randomly in time (see, for example, Japanese Patent Laid-Open No. 7-92656). Hereinafter, only the case of non-polarized light will be described in detail, but in the case of random polarized light, the same effect can be obtained with the same configuration.

  A turret 2 is provided in the optical path of the broadband light M. The turret 2 holds a plurality of filters 2a and 2b. Note that the number of filters held in the turret 2 is arbitrary. The turret 2 is rotated by a drive mechanism (see FIG. 2). Thereby, the plurality of filters 2a and 2b are alternatively arranged in the optical path.

  The configuration in which the plurality of filters 2a and 2b are alternatively arranged in the optical path is not limited to this. For example, it is possible to arrange a plurality of filters 2a and 2b along one direction and move them in that direction.

  The filter 2a is disposed in the optical path when acquiring an OCT image. The broadband light M output from the halogen lamp 1 includes light of various bands. The filter 2a is a filter that transmits only a predetermined band of the broadband light M. The band to be transmitted is determined according to the resolution, measurement depth, and the like, and is set to a band having a center wavelength of about 760 nm and a wavelength width of about 100 nm, for example. In this case, an image with a resolution of about 2 μm can be acquired in each of the depth direction of the object to be measured 1000 (z direction shown in FIG. 1) and the direction orthogonal to the depth direction (horizontal direction). The light transmitted through the filter 2a is also referred to as broadband light M.

  The filter 2b is disposed in the optical path during spectral imaging. The spectroscopic filter 2b transmits a predetermined wavelength component of the broadband light M. It is possible to provide other spectral imaging filters that transmit other wavelength components. The wavelength component that passes through the spectral imaging filter 2b and the like is a wavelength that reaches a desired depth of the object 1000 to be measured. Therefore, images of various depths of the measured object 1000 can be acquired by selectively arranging the spectral imaging filter 2b and the like in the optical path. The wavelength component transmitted through the spectroscopic filter 2b or the like may be light having a predetermined single wavelength, or may be light in a wavelength band having a predetermined width.

  First, the configuration of the optical image measurement device 100 will be described in consideration of the case where an OCT image is acquired. The non-polarized broadband light M transmitted through the filter 2a is split by a beam splitter 3 such as a half mirror. That is, the reflected light from the beam splitter 3 forms the signal light S, and the light transmitted through the beam splitter 3 forms the reference light R.

  The signal light S is focused on the object to be measured 1000 by the objective lens 11 while maintaining a non-polarized state. The signal light S is applied to the object to be measured 1000 with a predetermined beam diameter. The light reflected and scattered on the surface and inside of the measured object 1000 returns to the beam splitter 3 via the objective lens 11.

  On the other hand, the non-polarized reference light R generated by the beam splitter 3 is reflected by the reflection mirror 4 and passes through the wavelength plate (λ / 4 plate) 5 and the polarizing plate 6. Further, the reference light R passes through the glass plate 7 and is focused on the reflecting surface of the reference mirror 9 by the objective lens 8. The reference light R reflected by the reference mirror 9 returns to the beam splitter 3 via the same optical path in the reverse direction.

  At this time, the reference light R that was initially unpolarized is converted into circularly polarized light by passing through the wave plate 5 and the polarizing plate 6 twice. The glass plate 7 is a dispersion correction optical element that minimizes the influence of dispersion generated in the optical paths of the signal light S and the reference light R (both arms of the interferometer).

  The reference mirror 9 can be moved by the reference mirror moving mechanism 10 in the traveling direction of the reference light R, that is, in the direction orthogonal to the reflecting surface of the reference mirror 9 (in the direction of the double-sided arrow shown in FIG. The reference mirror moving mechanism 10 includes drive means such as a piezo element.

  By moving the reference mirror 9 in this way, the optical path length difference between the signal light S and the reference light R is changed. Here, the optical path length of the signal light S is a reciprocal distance between the beam splitter 3 and the surface of the object 1000 to be measured. The optical path length of the reference light R is a reciprocating distance between the beam splitter 3 and the reflecting surface of the reference mirror 9. By changing the optical path length difference between the signal light S and the reference light R, it is possible to selectively acquire images at various depth positions of the measured object 1000.

  In this embodiment, the optical path length difference is changed by changing the optical path length of the reference light R. However, the optical path length difference of the signal light S is changed to change the optical path length difference. It is also possible. In that case, a changing means for changing the distance between the apparatus optical system and the object 1000 to be measured is provided. For example, a stage that moves the apparatus optical system in the z direction, a stage that moves the measured object 1000 in the z direction, and the like can be applied.

  The signal light S that has passed through the measured object 1000 and the reference light R that has passed through the reference mirror 9 are superimposed by the beam splitter 3 to generate interference light L. The interference light L includes an S-polarized component and a P-polarized component.

  The interference light L generated by the beam splitter 3 passes through the aperture stop 12 and becomes focused light by the imaging lens (group) 13. The S-polarized component L1 of the interference light L that has become focused light is reflected by the polarization beam splitter 14 and detected by a CCD (image sensor) 17. On the other hand, the P-polarized component L2 of the interference light L is transmitted through the polarization beam splitter 14, reflected by the reflection mirror 16, and detected by a CCD (image sensor) 18.

  Each of the CCDs 17 and 18 has a two-dimensional light receiving surface. The S-polarized component L1 and the P-polarized component L2 are projected onto the light receiving surfaces of the CCDs 17 and 18 in a state having a predetermined beam diameter, respectively.

  The CCD 17 that has detected the S-polarized component L 1 sends a detection signal to the computer 20. Similarly, the CCD 18 that has detected the P-polarized component L 2 sends a detection signal to the computer 20.

Since the reference light R that is the source of the interference light L is circularly polarized and the signal light S is non-polarized, the S-polarized component L1 and the P-polarized component L2 have a phase difference of 90 degrees (π / 2). ing. Therefore, a detection signal _{C A} output from the CCD 17, the detection signal _{C B} outputted from the CCD18 has a phase difference of 90 degrees. Such detection signals C _A and C _B can be expressed as follows.

Here, I _s (x, y) represents the intensity of the signal light S, and I _r (x, y) represents the intensity of the reference light R. Φ (x, y) represents an initial phase difference. Each detection signal C _A , C _B includes a background light component (non-interference component, DC component) I _s (x, y) + I _r (x, y). Further, the detection signal C _A includes an interference component composed of a cos component, and the detection signal C _B includes an interference component composed of a sin component.

As can be seen from the equations (1) and (2), each of the detection signals C _A and C _B includes only space (x direction orthogonal to the z direction, y direction) as a variable, and does not include time as a variable. That is, the interference signal according to the present embodiment includes only a spatial change. Processing for forming an image based on the detection signals C _A and C _B will be described later.

  As described above, the polarization beam splitter 14 is disposed in the optical path (interference optical path) of the interference light L when acquiring an OCT image. On the other hand, at the time of spectral imaging, the polarization beam splitter 14 is retracted from the optical path, and an optical path length correction member 15 is disposed instead.

  The optical path length correction member 15 is a translucent member such as a glass block, for example, and corrects the optical path length of light projected on the CCD 18 via the object to be measured 1000. The optical path length extended by the optical path length correcting member 15 is equal to that of the polarizing beam splitter 14, for example. Instead of providing the optical path length correction member 15, the CCD 18 may be moved by a distance that compensates for the optical path length shortened by retracting the polarizing beam splitter 14. By correcting the optical path length with these configurations, the light passing through the object to be measured 1000 is appropriately projected onto the CCD 18. In the spectral imaging according to this embodiment, the CCD 17 is not used.

  Hereinafter, the configuration of the optical image measurement device 100 will be described in consideration of the case where a spectral image is acquired. In this embodiment, the broadband light M output from the halogen lamp 1 is used also in spectroscopic imaging. That is, the broadband light M is used as “photographing light” of the present invention. Note that the beam diameter of the broadband light M may be different between when an OCT image is acquired and when spectral imaging is performed. It is desirable that the beam diameter of the broadband light M can be arbitrarily changed by a known optical system.

  Spectral imaging can also be performed using light other than the band light M. As an example, it is possible to separately provide a broadband light source other than the halogen lamp 1 and selectively use them. The light source for spectroscopic imaging is not limited to a broadband light source, and it is sufficient that light including a spectral imaging wavelength band can be output.

  When performing spectroscopic imaging, the optical path (reference optical path) of the reference light R, that is, the optical path connecting the beam splitter 3 and the reference mirror 9 is blocked. In this embodiment, the reference optical path is blocked by moving the reflecting mirror 4. Here, “cut off the reference optical path” means that light that has passed through the beam splitter 3 and entered the reference optical path does not (almost) return to the beam splitter 3. In this embodiment, the optical path (signal optical path) of the signal light S is left as it is.

  In the optical image measurement device 100, the reference optical path is blocked by moving the reflection mirror 4. A specific example will be described. First, the reflecting mirror 4 is movably held by a holding member such as a rotating shaft member or a rail member (not shown). The drive mechanism 19 generates a driving force by an actuator such as a motor, and transmits the driving force to the reflection mirror 4, thereby rotating the reflection mirror 4 around the rotation shaft member or reflecting the reflection mirror along the rail member. 4 is retreated from the reference light path. Note that the reflection mirror 4 indicated by a dotted line in FIG. 1 represents a state where it is retracted from the reference optical path. In addition, the drive mechanism 19 moves the retracted reflection mirror 4 to place it in the reference optical path.

  The spectral imaging filter 2b (or other spectral imaging filter) transmits only a predetermined wavelength component of the broadband light M output from the halogen lamp 1. This wavelength component is divided into two by the beam splitter 3.

  The wavelength component that has passed through the beam splitter 3 and entered the reference optical path does not return to the beam splitter 3 because the reflecting mirror 4 is retracted.

  On the other hand, the wavelength component reflected by the beam splitter 3 is focused by the objective lens 11 and projected onto the object 1000 to be measured. This wavelength component is projected on the measurement object 1000 with a predetermined beam diameter, and reaches a depth corresponding to the wavelength. The wavelength component reflected at this depth returns to the beam splitter 3 via the objective lens 11.

  Further, the wavelength component that has passed through the object 1000 to be measured passes through the beam splitter 3, passes through the aperture stop 12, and becomes focused light by the imaging lens (group) 13. Further, this wavelength component is reflected by the reflection mirror 16 via the optical path length correction member 15 and projected onto the light receiving surface of the CCD 18.

[Control system configuration]
The configuration of the control system of the optical image measurement device 100 will be described. FIG. 2 illustrates a configuration example of a control system of the optical image measurement device 100.

  The computer 20 includes a control unit 21, a display unit 22, an operation unit 23, and a signal processing unit 24.

(Control part)
The control unit 21 controls each unit of the optical image measurement device 100. For example, the control unit 21 controls turning on / off of the halogen lamp 1, controlling the reference mirror moving mechanism 10, controlling the exposure time of the CCDs 17 and 18, controlling the driving mechanism 19, and controlling display processing by the display unit 22. Do.

  The optical image measurement device 100 is provided with drive mechanisms 25 and 26. The drive mechanism 25 rotates the turret 2 to selectively place a plurality of filters 2a, 2b, etc. in the optical path. The drive mechanism 26 moves the polarization beam splitter 14 and the optical path length correction member 15 and selectively arranges them in the optical path. These drive mechanisms 25 and 26 are configured to include an actuator such as a motor that generates a driving force.

  The control unit 21 includes a microprocessor such as a CPU. The control unit 21 includes a storage device such as a RAM, a ROM, and a hard disk drive. A computer program (not shown) for device control is stored in advance in the hard disk drive. Control by the control unit 21 is realized by the microprocessor operating in accordance with this computer program.

  The control unit 21 may include a communication device for performing data communication with an external device. Communication devices include LAN cards and modems. Thereby, the control part 21 can acquire various information from an external database, or can register information in a database. In addition, the control unit 21 can acquire information from an external device or transmit information to the external device.

(Display section)
The display unit 22 is controlled by the control unit 21 to display various information. The display unit 22 includes an arbitrary display device such as an LCD or a CRT display.

(Operation section)
The operation unit 23 is used by an operator to operate the optical image measurement device 100 and input various kinds of information. The operation unit 23 includes an arbitrary operation device and an input device such as a mouse, a keyboard, a joystick, a trackball, and a dedicated control panel.

(Signal processing part)
The signal processing unit 24 processes various signals. In particular, the signal processing unit 24 forms an image of the measured object 1000 based on the detection signals C _A and C _B output from the CCDs 17 and 18. This image forming process will be described later.

  The signal processing unit 24 includes a microprocessor such as a CPU, a RAM, a ROM, a hard disk drive, and the like.

[Mode of operation]
An operation mode of the optical image measurement device 100 will be described. The flowchart shown in FIG. 3 represents an operation example in the case of performing spectral imaging after acquiring an OCT image. In addition, when acquiring an OCT image after performing a spectroscopic imaging, the switching control contrary to FIG. 3 may be appropriately executed.

  First, the object to be measured 1000 is arranged at a predetermined measurement position, and alignment (alignment) of the apparatus optical system with respect to the object to be measured 1000 is performed (S1). For example, when the object 1000 to be measured is a living eye, the subject's face is supported by a forehead or a chin rest, and the eye to be examined is placed in front of the objective lens 11. Then, alignment is performed by moving the optical system of the optical image measurement apparatus 100 up and down, back and forth, and left and right. When the object to be measured 1000 is an object such as an extracted eye, this object is placed on a stage, and this stage is moved up and down, back and forth, and left and right to perform alignment. At this time, alignment may be performed by moving the optical system of the optical image measurement device 100.

  Next, a horizontal tomographic image (OCT image) of the measurement object 1000 is formed (S2). Hereinafter, an image forming operation will be described.

  When the operator performs a predetermined measurement start operation using the operation unit 23, the control unit 21 turns on the halogen lamp 1. In this operation mode, the continuous light of the broadband light M is output with the halogen lamp 1 turned on.

Next, the control unit 21 controls the reference mirror moving mechanism 10 to set the optical path length of the reference light R to the first optical path length. The first optical path length corresponds to the first depth position (z coordinate value) of the object 1000 to be measured. The control unit 21 controls the exposure time of each of the CCDs 17 and 18. The CCDs 17 and 18 output interference light detection signals C _A and C _B , respectively.

Next, the control unit 21 controls the reference mirror moving mechanism 10 to switch the optical path length of the reference light R to the second optical path length. The second optical path length corresponds to the second depth position of the measured object 1000. The control unit 21 controls the exposure times of the CCDs 17 and 18 to output new detection signals C _A ′ and C _B ′.

Here, the first optical path length and the second optical path length are such that the detection signal C _A and the detection signal C _A ′ have a phase difference of 180 degrees (π), and the detection signal C _B and the detection signal C _B. Is set in advance so as to be a distance interval having a phase difference of 180 degrees (π). Since the detection signals C _A and C _B have a phase difference of 90 degrees, four detection signals C _A , C _B , C _A ′, and C _B ′ are obtained for each phase difference of 90 degrees. .

The signal processing unit 24 adds the detection signals C _A and C _A ′ (phase difference 180 degrees), and divides the sum by 2 to obtain the background light component I _s (x, y) + I _r (x, y ) Is calculated. This calculation process may be performed using the detection signals C _B and C _B ′ (phase difference 180 degrees).

Further, the signal processing unit 24 subtracts the background light component I _s (x, y) + I _r (x, y) from each of the detection signals C _A and C _B to obtain an interference component (cos component, sin component). Then, the signal processing unit 24 forms an image in a cross section in the xy direction (horizontal direction) by calculating the square sum of the interference components of the detection signals C _A and C _B. This process may be performed using the detection signals C _A ′ and C _B ′ (phase difference 180 degrees).

  The controller 21 sequentially forms the xy cross-sectional images at various depth positions of the measured object 1000 by changing the optical path length of the reference light R and repeating the above processing. Thereby, for example, when acquiring an image of the cornea, images of various depth positions of the surface layer cell, the wing cell, the basal cell, the Bowman layer, and the corneal stroma are obtained.

  In this process, the control unit 21 controls the CCDs 17 and 18 to output detection signals at a predetermined frame rate and at the same timing. Further, the control unit 21 synchronizes the frame rate, the exposure timing of the CCDs 17 and 18, the movement timing of the reference mirror 9, and the change timing of the optical path length of the reference light R.

  At this time, the exposure time of each CCD 17 and 18 is set shorter than the frame rate. For example, the frame rate of the CCDs 17 and 18 can be set to 30 f / s, and the exposure time can be set to about 30 to 50 μs.

  Further, by using broadband light M having a center wavelength of about 760 nm and a wavelength width of about 100 nm, an image with a resolution of about several μm can be acquired. For example, assuming that the wavelength of the broadband light M is a Gaussian type and the refractive index of the measured object 1000 is n = 1.33, the theoretical value of the resolution is about 1.8 μm.

  The image of the measured object 1000 acquired in this way is stored in, for example, a storage device (described above) of the control unit 21.

  Further, the control unit 21 causes the display unit 22 to display a horizontal tomographic image of the measured object 1000 in response to an operation on the operation unit 23, for example.

  In addition, the signal processing unit 24 arranges a plurality of horizontal tomographic images along the z direction, or performs an interpolation process on the plurality of horizontal tomographic images to thereby obtain a three-dimensional image of the object 1000 to be measured. Can be formed. Furthermore, the signal processing unit 24 can form a tomographic image at an arbitrary cross section of the measured object 1000 based on a plurality of horizontal tomographic images or three-dimensional images. The control unit 21 can display these three-dimensional images and tomographic images on the display unit 22. This completes the process for forming the OCT image of the object 1000 to be measured.

  The operator requests switching from OCT measurement to spectral imaging by performing a predetermined operation using the operation unit 23 (S3).

  In response to this switching request, the control unit 21 controls the drive mechanism 19 to retract the reflecting mirror 4 from the reference optical path (S4), controls the drive mechanism 25 to retract the filter 2a from the optical path, and moves the filter 2b to the optical path. (S5), the drive mechanism 26 is controlled to retract the polarizing beam splitter 14 from the optical path, and the optical path length correction member 15 is disposed in the optical path (S6). This prepares for spectroscopic photography. In addition, the order which performs step 4-step 6 is arbitrary. It is also possible to execute any two or more of steps 4 to 6 at the same time.

  The operator operates the operation unit 23 to request the start of spectral imaging. In response to this request, the control unit 21 causes the subject 1000 to perform spectral imaging (S7). That is, the control unit 21 causes the halogen lamp 1 to output the broadband light M (imaging light) and controls the CCD 18 to detect the imaging light that has passed through the object 1000 to be measured. Then, the control unit 21 displays a spectral image of the measured object 1000 on the display unit 22 based on the signal from the CCD 18. In addition, the control unit 21 stores the spectral image in a storage device (described above). This is the end of this operation mode.

[Action / Effect]
The operation and effect of the optical image measurement device 100 will be described.

  The optical image measurement apparatus 100 functions as a full field type OCT apparatus. That is, the optical image measurement device 100 divides the broadband light M having a predetermined beam diameter into the signal light S and the reference light R, and the reference light via the signal light S and the reference mirror 9 passing through the measured object 1000. The interference light L is generated by causing interference with R, and the interference light L is detected to form an OCT image of the object 1000 to be measured. The formed OCT image is a tomographic image or a three-dimensional image of the object 1000 to be measured.

  Furthermore, the optical image measurement device 100 can also acquire other types of images of the object to be measured 1000 (types of images other than OCT images). In particular, the optical image measurement device 100 can capture a spectral image of the object 1000 to be measured. For this purpose, the optical image measurement apparatus 100 includes an output unit, a blocking unit, an optical system, and an image forming unit.

  The output means outputs photographing light for acquiring the other types of images. In this embodiment, the halogen lamp 1 and the filter 2b function as output means, and output photographing light including a predetermined wavelength component.

  The blocking means blocks the reference light path at least when photographing light is output. In this embodiment, a beam splitter 3 (dividing member) that divides the broadband light M into the signal light S and the reference light R is provided. Further, on the reference optical path, the reference mirror 9 as a reference object and the reference light R divided from the broadband light M by the beam splitter 3 are reflected toward the reference mirror 9, and the reference light R reflected by the reference mirror 9 is reflected. And a reflecting mirror 4 (reflecting mirror) for reflecting the beam toward the beam splitter 3. The drive mechanism 19 blocks the reference light path by moving the reflection mirror 4 and retracting the reflection mirror 4 from the reference light path. It is also possible to block the reference light path by changing the direction of the reflection mirror 4 so that the reference light R does not return to the beam splitter 3.

  The optical system irradiates the measured object 1000 with the output photographing light through the signal light path, and guides the photographing light through the measured object 1000 through the signal light path and the interference light path. In this embodiment, the beam splitter 3, the objective lens 11 (aperture stop 12), the imaging lens 13, the optical path length correction member 15, and the reflection mirror 16 function as an optical system.

  The image forming means detects photographing light guided by the optical system and forms an image of the object to be measured 1000. In this embodiment, the CCD 18 functions as image forming means. Various image processing can be performed by the signal processing unit 24 on the image formed by the CCD 18.

  According to such an optical image measurement apparatus 100, in addition to the OCT image of the object to be measured 1000, it is possible to acquire other types of images of the object to be measured, particularly spectral images. In particular, it is possible to acquire an OCT image and other types of images with a relatively simple configuration.

  In addition, the optical image measurement device 100 can capture images of various depths of the object to be measured 1000 by selectively arranging a plurality of filters 2b or the like that transmit different wavelength components in the optical path. is there. Here, the switching arrangement of the filter 2b and the like is executed by the control unit 21. The control unit 21 is an example of the “control unit” in the present invention.

[Modification]
A modification of the optical image measurement device 100 will be described.

  In the above embodiment, the photographing light for spectral photographing is output by a combination of a light source that generates broadband light and a filter, but the present invention is not limited to this. For example, an “output unit” that includes a wavelength tunable light source that can generate a plurality of imaging lights of different wavelengths and a control unit that controls the wavelength tunable light source to sequentially generate a plurality of wavelengths of imaging light is applied. Is possible. As a wavelength variable light source, for example, one disclosed in Japanese Patent Application Laid-Open No. 2008-47730 is known. Moreover, the control part 21 performs control of a wavelength variable light source, for example.

  It is also possible to apply an “output unit” including a plurality of light sources that output photographing light of different wavelengths and a control unit that selectively outputs the photographing light to these light sources. In addition, the control part 21 performs control of a several light source, for example.

  In the above embodiment, the reference optical path is blocked by moving the reflecting mirror 4, but the present invention is not limited to this. For example, it is possible to provide a shutter in the reference optical path, open the shutter during OCT measurement, and close the shutter during spectral imaging. The shutter is desirably arranged obliquely with respect to the traveling direction of the reference light (the optical axis of the reference light path). This is to prevent the reflected light from the surface of the shutter from returning to the beam splitter 3.

  In the above embodiment, spectral imaging is performed by using imaging light of a predetermined wavelength component, but the present invention is not limited to this. The optical image measurement device 200 shown in FIG. 4 has a configuration different from that of the above embodiment. The optical image measurement device 200 has a control system similar to that of the optical image measurement device 100 (see FIG. 2). In addition, the same code | symbol is used about the component similar to said embodiment.

  In the optical image measurement device 200, the filter 2a used for OCT measurement can be inserted into and removed from the optical path of the broadband light M. The insertion / removal operation of the filter 2a is executed by the same drive mechanism 25 as in the above embodiment. In addition, the drive mechanism 25 according to this modification may move the filter 2a linearly. For example, the filter 2a can be linearly moved using an actuator such as a solenoid or a motor.

  The optical image measurement device 200 includes the same drive mechanism 19 as in the above-described embodiment, and can retract the reflection mirror 4 from the reference light path.

  Further, the optical image measurement device 200 includes a filter 30. The filter 30 is a filter that transmits a predetermined wavelength component, like the filter 2b and the like of the above-described embodiment. That is, the filter 30 acts to extract a predetermined wavelength component from the broadband light M (imaging light) that has passed through the measured object 1000. The filter 30 is an example of the “extraction means” in the present invention.

  The drive mechanism 26 alternatively arranges the polarization beam splitter 14 and the filter 30 in the optical path of the interference light L. Here, it is possible to provide a plurality of filters 30 and the like that transmit different wavelength components. In this case, the drive mechanism 26 selectively arranges the polarization beam splitter 14 and the plurality of filters 30 in the optical path.

  The filter 30 acts to correct the optical path length of the light traveling toward the CCD 18 in the same manner as the optical path length correction member 15 of the above embodiment. An optical path length correction member can be provided separately from the filter 30 and can be arranged in the optical path during spectroscopic imaging.

  The operation mode of the optical image measurement apparatus 200 will be described. The flowchart shown in FIG. 5 represents an operation example when performing spectral imaging after acquiring an OCT image. In addition, what is necessary is just to perform switching control contrary to FIG. 5 suitably, when acquiring an OCT image after performing spectral imaging.

  First, similarly to the above-described embodiment, the measurement object 1000 is placed at a predetermined measurement position, alignment with the measurement object 1000 is performed (S21), and a horizontal tomographic image (OCT image) of the measurement object 1000 is obtained. Form (S22).

  When the OCT image is acquired, the operator performs a predetermined operation using the operation unit 23 to request switching from OCT measurement to spectral imaging (S23).

  Upon receiving this switching request, the control unit 21 controls the drive mechanism 19 to retract the reflection mirror 4 from the reference optical path (S24), and controls the drive mechanism 25 to retract the filter 2a from the optical path (S25). The mechanism 26 is controlled to retract the polarizing beam splitter 14 from the optical path and arrange the filter 30 in the optical path (S26). This prepares for spectroscopic photography. In addition, the order which performs step 24-step 26 is arbitrary. It is also possible to execute any two or more of steps 24 to 26 simultaneously.

  The operator operates the operation unit 23 to request the start of spectral imaging. In response to this request, the control unit 21 causes the subject 1000 to perform spectral imaging (S27).

  In this spectroscopic imaging, broadband light M is used as imaging light. When the control unit 21 controls the halogen lamp 1 to output the broadband light M, the broadband light M is split by the beam splitter 3. The broadband light M that has entered the reference optical path does not return to the beam splitter 3 because the reflecting mirror 4 is retracted. On the other hand, the broadband light M reflected by the beam splitter 3 is projected onto the measurement object 1000 by the objective lens 11. Various wavelength components included in the broadband light M reach a depth corresponding to the wavelength and are reflected. The broadband light M reflected by the object 1000 to be measured enters the filter 30 via the objective lens 11, the beam splitter 3, the aperture stop 12, and the imaging lens 13. The filter 30 transmits a predetermined wavelength component of the broadband light M. The wavelength component transmitted through the filter 30 is reflected by the reflection mirror 16 and detected by the CCD 18. The control unit 21 causes the display unit 22 to display a spectral image of the measured object 1000 based on the signal from the CCD 18. In addition, the control unit 21 stores the spectral image in a storage device (described above). This is the end of this operation mode.

  According to such an optical image measurement device 200, in addition to the OCT image of the object to be measured 1000, it is possible to acquire other types of images of the object to be measured, particularly spectral images. In particular, it is possible to acquire an OCT image and other types of images with a relatively simple configuration.

  Further, the optical image measurement device 200 can take images of various depths of the measured object 1000 by selectively arranging a plurality of filters 30 and the like that transmit different wavelength components in the optical path. is there. Here, the switching arrangement of the filter 30 and the like is executed by the control unit 21. The control unit 21 is an example of the “control unit” in the present invention.

  The optical image measurement device 200 includes an extraction unit that transmits a predetermined wavelength component included in the broadband light M (imaging light) that has passed through the object 1000 to be measured. However, an extraction unit that is different from this may be provided. It is.

  For example, a member (dispersing member) that disperses the broadband light M that has passed through the measured object 1000 into a plurality of wavelength components can be used as the extracting means. As the dispersion member, any optical member such as a diffraction grating, a prism, or a PGP (prizm gratting prism) can be used. In this modification, the optical system is configured so that the CCD 18 detects a predetermined wavelength component. Thereby, a spectral image based on a predetermined wavelength component can be acquired. Further, by configuring the wavelength component to be detected by the CCD 18 so as to be changeable, it is possible to selectively acquire spectral images of various depths of the measured object 1000.

  It is also possible to configure such that a plurality of wavelength components can be detected simultaneously. For example, when the CCD 18 is a 3CCD type color image sensor, it is possible to simultaneously detect the R (red) component, G (green) component, and B (blue) component of the broadband light M that has passed through the object 1000 to be measured. is there. Thereby, a plurality of spectral images corresponding to a plurality of depths of the measured object 1000 can be acquired simultaneously.

<Second Embodiment>
[Constitution]
The optical image measurement device according to this embodiment is a device that can acquire an OCT image and a fluorescence image. A configuration example of the optical image measurement device according to this embodiment is shown in FIG. The optical image measurement apparatus 300 functions as a full field type OCT apparatus. The optical image measurement device 300 has a control system similar to that of the first embodiment (see FIG. 2). In addition, the same code | symbol is used about the component similar to 1st Embodiment.

  The turret 2 of the optical image measuring device 300 holds an OCT measurement filter 2a and an exciter filter 2c for fluorescence imaging. The exciter filter 2 c transmits the excitation wavelength component of the broadband light M generated by the halogen lamp 1. The excitation wavelength component is light having a wavelength that excites a predetermined fluorescent substance in the object to be measured 1000 to generate fluorescence. The drive mechanism 25 drives the turret 2 to selectively place the filter 2a and the exciter filter 2c in the optical path.

  Here, a plurality of exciter filters 2c having different excitation wavelength components may be provided. For example, when the object to be measured 1000 is a fundus, it is possible to provide an exciter filter for photographing fluorescein (FAG) fluorescent fundus and an exciter filter for photographing indocyanine green (ICG) fluorescent fundus. When a plurality of exciter filters 2c and the like are provided, the drive mechanism 25 alternatively arranges the filter 2a and the plurality of exciter filters 2c and the like in the optical path.

  The optical image measurement device 300 is provided with a barrier filter 40. The barrier filter 40 is a filter that blocks wavelength components other than the fluorescence emitted from the fluorescent material excited by the excitation wavelength component of the broadband light M, that is, transmits the wavelength component corresponding to the fluorescence. The drive mechanism 26 selectively arranges the polarization beam splitter 14 and the barrier filter 40 in the optical path. The barrier filter 40 has the same function as the optical path length correction member 15 of the first embodiment. In addition, an optical path length correction member may be provided separately from the barrier filter 40 and arranged in the optical path during fluorescence imaging.

  When a plurality of exciter filters 2c and the like are provided, a plurality of barrier filters 40 and the like corresponding to the plurality of exciter filters 2c and the like are provided. The drive mechanism 26 selectively arranges the polarization beam splitter 14 and the plurality of barrier filters 40 in the optical path. At this time, the barrier filter 40 or the like corresponding to the type of the exciter filter 2c or the like is selectively disposed.

[Mode of operation]
The operation mode of the optical image measurement apparatus 300 will be described. The flowchart shown in FIG. 7 represents an operation example in the case of performing fluorescence imaging after acquiring an OCT image. In addition, what is necessary is just to perform switching control contrary to FIG. 7 suitably, when acquiring an OCT image after performing fluorescence imaging | photography.

  It is assumed that a fluorescent material is applied to the object to be measured 1000. For example, in fluorescent fundus photography, a fluorescent agent containing a predetermined fluorescent substance is intravenously injected to a subject.

  First, as in the first embodiment, the object to be measured 1000 is arranged at a predetermined measurement position, aligned with the object to be measured 1000 (S41), and a horizontal tomographic image (OCT image) of the object to be measured 1000 is obtained. Is formed (S42).

  When the OCT image is acquired, the operator performs a predetermined operation using the operation unit 23 to request switching from OCT measurement to fluorescence imaging (S43).

  In response to this switching request, the control unit 21 controls the drive mechanism 19 to retract the reflecting mirror 4 from the reference optical path (S44), and controls the drive mechanism 25 to retract the filter 2a from the optical path and to move the exciter filter 2c. It arrange | positions to an optical path (S45), the drive mechanism 26 is controlled, the polarizing beam splitter 14 is evacuated from an optical path, and the barrier filter 40 is arrange | positioned to an optical path (S46). This prepares for fluorescence photography. In addition, the order which performs step 44-step 46 is arbitrary. It is also possible to execute any two or more of steps 44 to 46 at the same time. Further, depending on the type of fluorescent photographing, the light source is switched to a xenon lamp or the like.

  The operator operates the operation unit 23 to request the start of fluorescence imaging. In response to this request, the control unit 21 causes the subject 1000 to perform fluorescence imaging (S47). When acquiring a plurality of images at appropriate timing as in fluorescent fundus imaging, the operator requests imaging at a desired timing, and the control unit 21 causes the light source to output imaging light accordingly.

  In this fluorescent photographing, the broadband light M is used as photographing light. When the control unit 21 controls the halogen lamp 1 (or another light source) to output the broadband light M, only the excitation wavelength component transmitted through the exciter filter 2 c reaches the beam splitter 3. The excitation wavelength component that has passed through the beam splitter 3 and entered the reference optical path does not return to the beam splitter 3 because the reflection mirror 4 is retracted. On the other hand, the excitation wavelength component reflected by the beam splitter 3 is projected onto the measurement object 1000 by the objective lens 11. The excitation wavelength component projected on the measurement object 1000 excites the fluorescent material applied to the measurement object 1000. The excited fluorescent material emits fluorescence. This fluorescence enters the barrier filter 40 via the objective lens 11, the beam splitter 3, the aperture stop 12, and the imaging lens 13. Note that light having a wavelength other than fluorescence (for example, a wavelength component) also reaches the barrier filter 40. The barrier filter 40 blocks light having a wavelength other than fluorescence. The fluorescence transmitted through the barrier filter 40 is reflected by the reflection mirror 16 and detected by the CCD 18. Based on the signal from the CCD 18, the control unit 21 forms an image (distribution image) representing the distribution of the fluorescent substance in the object 1000 to be measured. For example, in fluorescent fundus photography, this distribution image is an image representing the morphology of the fundus blood vessel. The control unit 21 displays the fluorescent image on the display unit 22. In addition, the control unit 21 stores this fluorescent image in a storage device (described above). This is the end of this operation mode.

  According to such an optical image measurement apparatus 200, in addition to the OCT image of the object to be measured 1000, it is possible to acquire other types of images of the object to be measured, particularly fluorescence images. In particular, it is possible to acquire an OCT image and other types of images with a relatively simple configuration.

  Further, the optical image measurement device 200 can deal with various fluorescent materials by arranging a plurality of exciter filters 2c and the like that transmit different excitation wavelength components and a plurality of barrier filters 40 and the like in the optical path. It is possible to take a distribution image. Here, the switching arrangement of the filter 2c and the like and the barrier filter 40 and the like is executed by the control unit 21.

[Modification]
As a modification of the optical image measurement device 300, a configuration applicable to spontaneous fluorescence imaging will be described (see, for example, Patent Document 4). In spontaneous fluorescence photography, it is not necessary to arrange the exciter filter 2c and the like in the optical path. Therefore, when switching from OCT measurement to spontaneous fluorescence imaging, (1) the reflection mirror 4 is retracted from the reference optical path, (2) the OCT measurement filter 2a is retracted from the optical path, and (3) the polarization beam splitter 14 is While being retracted from the optical path, an exciter filter 2c for spontaneous fluorescence photography is disposed in the optical path. According to such an optical image measurement device, it is possible to acquire a spontaneous fluorescence image in addition to the OCT image of the object to be measured 1000 (fundus, etc.). In particular, it is possible to acquire an OCT image and other types of images with a relatively simple configuration.

  In the optical image measurement device 300, the reference optical path is blocked by retracting the reflecting mirror 4 from the reference optical path. However, as in the modification of the first embodiment, the reference optical path is blocked using a shutter. It may be configured.

<Other variations>
In the above-described embodiment, the optical image measurement device capable of acquiring a spectral image or a fluorescence image as a type of image other than the OCT image has been described. However, it is also possible to apply a configuration for acquiring other types of images. is there. For example, the structure which can acquire the phase difference image by a phase contrast microscope with an OCT image is employable. At this time, if the apparatus is installed in an incubator for cell culture, it is possible to observe and evaluate the cell growth process.

  For example, in an optical image measurement device capable of acquiring a phase difference image, a mechanism for arranging ring slits in each of the optical path of the signal light S and the optical path of the interference light L is provided. When acquiring the phase difference image, these ring slits are arranged in the optical path, the reference optical path is blocked, and the OCT measurement filter 2a and the polarization beam splitter are retracted from the optical path, respectively.

  It is also possible to adopt a configuration that can acquire a differential interference image by a differential interference microscope together with an OCT image. When acquiring a differential interference image, a member for OCT measurement is retracted from the optical path, and a polarizing filter, a Wollaston prism (Wollaston prism), and the like are disposed in the optical path.

  Note that the phase contrast microscope and the differential interference microscope are suitably used for photographing a measurement object with high transparency such as cultured cells and cornea (for example, Japanese Patent Application Laid-Open No. 2007-219319 and Japanese Patent Application Laid-Open No. 2008-58852). See the publication).

  The type of image (image other than the OCT image) acquired by the optical image measurement device according to the present invention is not limited to the various examples described above, and may be any type of image.

  When the above-described wavelength tunable light source is used in OCT measurement, an OCT image suitable for a depth to be noticed (for example, an OCT in which the depth to be noticed is clearly depicted) in consideration of the fact that the depth of light varies depending on the wavelength. Image).

  In addition, since the resolution lc in the depth direction is expressed by the following equation (3), it is possible to maintain and adjust the resolution according to the change in wavelength by considering this relationship. In the following equation (3), λ0 represents the center wavelength of the light emitted from the light source, and Δλ represents the half width of the wavelength of the light emitted from the light source.

It is a schematic block diagram showing an example of a structure of embodiment of the optical image measuring device concerning this invention. It is a schematic block diagram showing an example of a structure of the control system of embodiment of the optical image measuring device which concerns on this invention. It is a flowchart showing an example of the operation | movement aspect of embodiment of the optical image measuring device concerning this invention. It is a schematic block diagram showing an example of a structure of the modification of embodiment of the optical image measuring device which concerns on this invention. It is a flowchart showing an example of the operation | movement aspect of the modification of embodiment of the optical image measuring device which concerns on this invention. It is a schematic block diagram showing an example of a structure of embodiment of the optical image measuring device concerning this invention. It is a flowchart showing an example of the operation | movement aspect of embodiment of the optical image measuring device which concerns on this invention.

Explanation of symbols

100, 200, 300 Optical image measuring device 1 Halogen lamp 2 Turrets 2a, 2b, 30 Filter 2c Exciter filter 3 Beam splitter 4, 16 Reflecting mirror 5 Wave plate 6 Polarizing plate 7 Glass plate 8, 11 Objective lens 9 Reference mirror 10 Mirror moving mechanism 12 Aperture stop 13 Imaging lens (group)
14 Polarizing beam splitter 15 Optical path length correction member 17, 18 CCD
19, 25, 26 Drive mechanism 20 Computer 21 Control unit 22 Display unit 23 Operation unit 24 Signal processing unit 40 Barrier filter

Claims (17)

Broadband light having a predetermined beam diameter is divided into signal light and reference light, and the signal light passing through the object to be measured via the signal light path interferes with the reference light passing through the reference object via the reference light path An optical image measurement device that generates interference light and detects the interference light guided through an interference optical path to form an image of the object to be measured,
Blocking means for blocking the reference optical path;
Output means for outputting photographing light in a state where the reference optical path is blocked;
An optical system that irradiates the object to be measured with the imaging light output from the output unit via the signal optical path, and guides the imaging light through the object to be measured through the signal optical path and the interference optical path; ,
Image forming means for forming an image by detecting photographing light via the optical system;
An optical image measurement device comprising: The output means outputs the photographing light including a predetermined wavelength component;
The image forming means detects the predetermined wavelength component via the optical system to form an image;
The optical image measuring device according to claim 1. The output means includes a light source that generates the broadband light, and a filter that transmits the predetermined wavelength component of the generated broadband light,
The optical system irradiates the object to be measured with the wavelength component transmitted through the filter as the imaging light,
The optical image measuring device according to claim 2. The output means includes a plurality of the filters that respectively transmit different wavelength components, and a control means that alternatively arranges the plurality of filters in an optical path of broadband light generated by the light source.
The optical image measuring device according to claim 3. The output means includes a wavelength tunable light source capable of generating a plurality of different wavelengths of imaging light, and a control means for controlling the wavelength variable light source to sequentially generate the plurality of different wavelengths of imaging light,
The optical image measuring device according to claim 2. The output means includes a plurality of light sources that output photographing light of different wavelengths, and a control means that selectively outputs the photographing light to the plurality of light sources.
The optical image measuring device according to claim 2. The output means includes a light source that generates the broadband light as the photographing light,
The optical system includes extraction means for extracting a predetermined wavelength component from the broadband light that has passed through the object to be measured,
The image forming means detects the extracted predetermined wavelength component to form an image;
The optical image measuring device according to claim 1. The extraction means includes a filter that transmits a predetermined wavelength component of the broadband light passing through the object to be measured,
The image forming unit forms an image by detecting a predetermined wavelength component transmitted through the filter;
The optical image measurement device according to claim 7. The extraction means includes a plurality of the filters that transmit different wavelength components, and a control means that alternatively arranges the plurality of filters in an optical path of the broadband light that passes through the object to be measured.
The optical image measuring device according to claim 8. The extraction means includes a dispersion member that disperses the broadband light passing through the object to be measured into a plurality of wavelength components,
The image forming unit detects one of the plurality of wavelength components to form an image;
The optical image measurement device according to claim 7. The output means outputs imaging light including an excitation wavelength component of a fluorescent substance in the object to be measured;
The optical system irradiates the object to be measured with imaging light including the excitation wavelength component, and guides fluorescence emitted from the fluorescent material upon receiving the imaging light through the signal optical path and the interference optical path. And
The image forming means detects fluorescence passing through the optical system and forms a distribution image of the fluorescent material on the object to be measured;
The optical image measuring device according to claim 1. The output means includes a light source that generates the broadband light, and an exciter filter that transmits the excitation wavelength component of the generated broadband light,
The optical system irradiates the object to be measured with the excitation wavelength component transmitted through the filter as the imaging light,
The optical system includes a barrier filter that blocks wavelength components other than the fluorescence,
The image forming means detects the fluorescence transmitted through the barrier filter and forms the distribution image;
The optical image measurement device according to claim 11. The output means includes a light source that generates the broadband light as the photographing light,
The optical system irradiates the object to be measured with broadband light generated by the light source,
The optical system includes a barrier filter that blocks wavelength components other than the fluorescence,
The image forming means detects the fluorescence transmitted through the barrier filter and forms the distribution image;
The optical image measurement device according to claim 11. The optical system includes ring slits in both the signal optical path and the interference optical path,
The image forming means detects imaging light passing through the optical system including both the ring slits to form a phase difference image;
The optical image measuring device according to claim 1. A splitting member that splits the broadband light into the signal light and the reference light;
In the reference light path, a reference mirror as the reference object, and the reference light divided from the broadband light by the dividing member is reflected toward the reference mirror, and the reference light reflected by the reference mirror is divided. And a reflecting mirror that reflects toward the member,
The blocking means includes a drive mechanism that moves the reflecting mirror,
The optical image measurement device according to claim 1, wherein the optical image measurement device is an optical image measurement device. The blocking means includes a shutter that blocks the reference light,
The optical image measurement device according to claim 1, wherein the optical image measurement device is an optical image measurement device. The shutter is provided obliquely with respect to the traveling direction of the reference light.
The optical image measurement device according to claim 16.

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