JP2009207394A - Microorganism-culturing sheet - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microorganism-culturing sheet usable for inspection or the like of the number of bacteria, having high performance, and facilitating the operation. <P>SOLUTION: The microorganism-culturing sheet comprises a base material sheet, a medium layer formed on the base material sheet, and a cover sheet for covering the medium layer. The medium layer is formed by coating a dispersion comprising a water-absorbing resin having ≤120 μm particle diameter and a water-soluble polymer in a mass ratio (water-absorbing resin/water-soluble polymer) within the range of 0.05-3, and a polar solvent except the water on the base material sheet. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a microorganism culture sheet that is used for testing the number of bacteria and the like, is excellent in water absorption amount and water absorption speed, is compact and easy to operate.

  As a method for confirming the presence of microorganisms or measuring the number of microorganisms, there is an agar plate pour method, and an agar medium formed in a petri dish previously sterilized is used. This method requires an autoclave for high-pressure steam sterilization of the agar medium and a laboratory that can perform microbiological tests aseptically. From microbial sampling to sample liquid preparation, dispensing, and mixing with the medium. Skillfulness is required for the operation of the microbiological examination from the culture to the counting. Therefore, a dry culture medium has been developed as a microorganism culture sheet that allows simple microorganism testing without requiring a high degree of skill.

  For example, a cold water-soluble powder containing a water-based adhesive composition layer formed on an upper surface of a support, a gelling agent attached to the adhesive composition layer, and the cold water-soluble powder are coated. There is a culture substrate device comprising a cover sheet (Patent Document 1). When the cover sheet is peeled off and the aqueous test sample is placed on a cold water soluble powder layer, the gelling agent quickly hydrates to form a reconstitution medium that maintains microbial growth, and covers the cover sheet for a specified time. When incubated, colonies can be counted through a transparent cover sheet. In Patent Document 1, xanthan gum, guar gum, caustic bean gum, carboxymethyl cellulose, hydroxyethyl cellulose, algin and the like are used as gelling agents.

  There is also a sheet-like culture container comprising a base material having one or more water-absorbing portions, and a portion other than the water-absorbing portions being water-repellent, and a film that seals the water-absorbing portions (Patent Document 2). ). It consists of a sheet-like base material coated with a medium and a water-absorbing resin, and a transparent film with a water-absorbing resin formed on the side of the base material. Since it can be cultured by sticking, it has the advantage of being lighter and less bulky than conventional Petri dishes and petri dishes. When the container is immersed in a liquid medium so that all the water absorption parts are peeled off and pulled up, the liquid medium is absorbed only in the water absorption part, and the liquid medium does not adhere to the water repellent parts other than the water absorption part. An independent culture medium can be obtained easily and reliably, and culture of microorganisms can be observed in this state if the water-absorbing part is sealed with a film after inoculating a desired microorganism into the medium.

In addition, in the microorganism culture sheet of a microorganism having a waterproof base sheet and a cover sheet placed on the base sheet, and at least a layer of the medium mixture is provided on the base sheet, the medium mixture There is also a microorganism culture sheet characterized in that an outer frame layer made of hydrophobic ink is provided on the layer to make the spread of the test solution to be inoculated constant (Patent Document 3). In the examples, a transparent biaxially stretched polyester film having a thickness of 100 μm is used as a base sheet, and a coating solution for the medium mixture layer is applied thereon with a comma coater so that the coating amount when dried is 30 g / m ^2. On the medium mixture layer, a donut-shaped pattern having an inner diameter of 50 mm and an outer diameter of 70 mm is screen-printed with a hydrophobic ink RAM (Seiko Advance Co., Ltd.) to form an outer frame layer.

Furthermore, there is a microorganism culture device in which a circular porous matrix layer and a water-soluble polymer compound layer are laminated on a square pressure-sensitive adhesive sheet, and a square transparent film is disposed on the top (Patent Document 4). When the sample solution is added onto the porous matrix layer, the sample solution is retained in the porous matrix layer, and the retained water dissolves the water-soluble polymer compound layer in contact with the porous matrix layer, resulting in water solubility. An environment in which microorganisms can grow is provided by the polymer compound aqueous solution and the growth nutrients. In this microorganism incubator, since the microorganisms do not penetrate into the water-soluble polymer compound layer and are further pushed up to the surface of the porous matrix layer, colonies are formed on or near the surface of the porous matrix layer, so that a transparent film It can be used as a sheet-shaped microorganism incubator by adhering to a pressure-sensitive adhesive sheet, and is lightweight, low in volume and easy to handle.
Japanese Patent Publication No. 3383304 JP-A-7-289235 JP-A-8-280377 International Publication No. WO01 / 044437

  The microorganism culture sheet is manufactured by forming a medium layer on a base sheet, but it is preferable that the manufacturing process is simple. However, although the said patent document 1 forms a water-based adhesive composition layer, since this adhesive composition layer contains water with a high boiling point, an excessive amount of drying energy is required, and the manufacturing time is extended. It contributes. Therefore, it is desired to develop a microorganism culture sheet that uses less water and a method for producing the same.

  In addition, a cold water-soluble powder containing a gelling agent is adhered onto the adhesive composition layer, but fine cold water-soluble powder is scattered in the bonding process, and is uniformly formed on the adhesive layer. It may be difficult to bond the cold water soluble powder. Therefore, it is desired to develop a microorganism culture sheet in which a medium layer is uniformly formed without scattering the gelling agent.

  In addition, since it is necessary to process a large number of specimens in the microbial test, it is preferable that they can be easily operated. However, the culture medium device described in Patent Document 1 is a device in which a cold water-soluble powder containing a gelling agent is attached to the entire surface of a water-based adhesive composition layer on a support. It is used by spreading the liquid. Therefore, a spreader for each specimen is required, and an operation for spreading the specimen on the cold water-soluble powder by the spreader is also necessary. Therefore, a microorganism culture sheet that can be used more quickly and easily is desired.

  On the other hand, Patent Document 2 uses a water-absorbing resin excellent in water-absorbing property. However, since a circular water-absorbing portion is formed on a water-repellent substrate so that the water-absorbing portion adheres to the water-repellent substrate. It is necessary to use something that can. In the examples, aqua coke dissolved in sodium polyacrylate, cyclohexanone or the like is used, but cyclohexanone has a higher boiling point than water and requires an excessive amount of energy for removing the solvent. Therefore, development of a microorganism culture sheet that is easy to remove the solvent and has excellent water absorption is desired.

  Furthermore, although the culture apparatus of Patent Document 3 forms a frame on the medium layer and makes the spread of the test solution to be inoculated constant, the medium layer is formed on the entire surface of the base sheet, The medium layer is wasted.

  The microorganism culture sheet is characterized in that it can be used easily, and it is preferable that the microorganism culture sheet can be manufactured at low cost. The microorganism incubator of Patent Document 4 requires at least two layers of a water-soluble polymer compound layer and a porous matrix layer. In addition, when the bacterial cells enter the porous matrix and the growth proceeds, it is difficult to see the generated colonies. In particular, when the bacterial cells proliferate close to each other, counting may be difficult.

  In view of the above problems, the present invention is excellent in water absorption magnification, and therefore can absorb the test solution uniformly without using a spreader or the like, and the test solution leaks from the end of the microorganism culture sheet. It aims at providing the microorganism culture sheet which does not have.

  Moreover, it aims at providing the manufacturing method of the microorganism culture sheet which can be manufactured with a simple manufacturing method, without using water.

  As a result of examining the microorganism culture sheet in detail, when a water-absorbent resin is used as a medium base material, the medium can be absorbed in the medium layer without using a spreader. The medium layer in which water-absorbing resin such as polyacrylic acid is uniformly dispersed can be prevented from leaking from the edge of the sheet. It can be formed by coating, and when a polar solvent other than water is used as the specific solvent, the solvent removal step can be facilitated while maintaining the water absorbing power of the water absorbent resin, and the specific water-soluble polymer is water-soluble and the above-mentioned Using a material that can be dissolved in a specific solvent makes it possible to form a uniform medium layer with excellent dispersibility of the water-absorbent resin, and the water-soluble polymer acts as a binder. Since the medium layer containing the water-absorbing resin gels when it absorbs water, it covers the medium layer when covered with a cover sheet, and the cover sheet is placed on the base sheet side without using other fixing members. It was found that when the frame layer was formed with UV foaming ink on the base material sheet, it was sufficient to form a medium layer only in the frame layer, and waste of the medium layer could be avoided, and the present invention was completed. .

  That is, the present invention is a microorganism culture sheet comprising a base sheet, a medium layer formed on the base sheet, and a cover sheet covering the medium layer, the medium layer having a particle diameter of 120 μm. A dispersion comprising the following water-absorbent resin and water-soluble polymer (water-absorbent resin / water-soluble polymer) in the range of 0.05 to 3, and a polar solvent other than water is used as the base sheet. The present invention provides a microorganism culture sheet characterized in that it is formed by coating on a microorganism.

  Further, a method for producing a microbial culture sheet comprising a base sheet, a medium layer formed on the base sheet, and a cover sheet covering the medium layer, the particle diameter on the base sheet A dispersion composed of a polar solvent other than water having a mass ratio of the water-absorbing resin of 120 μm or less to the water-soluble polymer (water-absorbing resin / water-soluble polymer) in the range of 0.05 to 3 is described above. The present invention provides a method for producing a microorganism culture sheet, comprising a step of coating a sheet, and then a step of removing the polar solvent contained in the dispersion.

  Furthermore, the present invention is a microbial culture sheet comprising a substrate sheet, a medium layer formed on the substrate sheet, and a cover sheet covering the medium layer, on the substrate sheet, A method for producing a microorganism culture sheet in which a frame layer made of UV foaming ink is formed, and the medium layer is formed in the frame layer, wherein a water-absorbing resin having a particle diameter of 120 μm or less and a water-soluble material are disposed inside the frame layer. A mass ratio (water-absorbent resin / water-soluble polymer) with a functional polymer is in the range of 0.05 to 3, and a step of applying a dispersion composed of a polar solvent other than water, and included in the dispersion Manufacturing a microorganism culture sheet, comprising: removing the polar solvent; forming a frame layer made of UV foamed ink on the base sheet; and irradiating the UV foamed ink with UV to foam A method is provided.

  According to the present invention, a water-absorbing resin is used as a medium base material, and a dispersion liquid in which the water-absorbing resin is dispersed in a characteristic solvent is used. The water absorbent resin can be dispersed in Since this dispersion contains a specific water-soluble polymer, it has excellent adhesion between the substrate sheet and the medium substrate, and the medium layer can be formed only by applying the dispersion.

The water-absorbing resin has a high water absorption amount and a high water absorption speed, and can quickly absorb the test liquid, so that leakage of the test liquid from the end of the microorganism culture sheet can be prevented.
In addition, since the microorganism culture sheet of the present invention has a cover sheet that covers the medium layer, it can prevent contamination due to falling bacteria during the culture operation, and at the same time, can prevent evaporation of moisture in the medium layer. .

  Furthermore, if a medium layer is formed on the inner side of the cover sheet at a position facing the medium layer, or an adhesive layer is formed at a position opposite to the frame layer, the cover sheet and the evaporation of moisture can be more reliably prevented. Culture accuracy can be improved.

  The first of the present invention is a microorganism culture sheet comprising a base sheet, a medium layer formed on the base sheet, and a cover sheet covering the medium layer, the medium layer having a particle size A dispersion composed of a polar solvent other than water having a mass ratio of the water-absorbing resin of 120 μm or less to the water-soluble polymer (water-absorbing resin / water-soluble polymer) in the range of 0.05 to 3 is described above. A microorganism culture sheet characterized by being formed by coating on a sheet. As the water-soluble polymer, polyvinyl pyrrolidone, polyethylene oxide, or hydroxypropyl cellulose can be suitably used. Moreover, as said polar solvent, ethyl alcohol, methyl alcohol, isopropyl alcohol, etc. can be used conveniently.

In the microorganism culture sheet of the present invention, a frame layer may be formed on a base material sheet, and a culture medium layer may be formed inside the frame layer.
In the microorganism culture sheet of the present invention, an adhesive layer may be formed on the cover sheet so as to face the frame layer formed on the base sheet. Furthermore, the cover sheet may be fixed to the base sheet.

(1) Structure of microorganism culture sheet An example of a preferred embodiment of the microorganism culture sheet of the present invention is shown in FIGS. FIG. 1A is a plan view, and FIG. 1B is a cross-sectional view taken along line AA ′ of FIG. FIG. 1 shows a mode in which a medium layer (30) is formed on the entire surface of a square base sheet (10) and a cover sheet (40) is provided so as to cover the medium layer (30). Has been. In the present invention, the shape of the base sheet (10) is not limited to a square, and may be any shape such as a circle, an ellipse, a polygon, and an indefinite shape.

  In the microorganism culture sheet of the present invention, a culture medium layer (30 ') may be formed on the cover sheet (40) as shown in the cross-sectional view of FIG. Since the culture medium layer (30) on the base sheet and the culture medium layer (30 ′) provided on the cover sheet (40) are arranged to face each other, for example, on the base sheet (10) When the culture medium layer (30) is inoculated with the cover sheet (40) and the culture medium layer (30) is covered with the cover sheet (40), the test liquid is absorbed into the culture medium layer (30, 30 ′) and both medium layers ( 30, 30 ') can grow microorganisms. Moreover, the culture medium base material which comprises a culture medium layer (30, 30 ') contains a water absorbing resin and a water-soluble polymer, and a culture medium layer (30, 30') exhibits adhesiveness by gelatinization. Since the medium layers (30, 30 ′) are in close contact with each other, the cover sheet (40) can be fixed to the base sheet (10) side without forming an adhesive layer or the like. Thereby, drying of a culture medium layer (30, 30 ') can be prevented, and by culture | cultivating microorganisms in the culture medium layer (30') on a cover sheet (40), from a cover sheet (40) side It is possible to facilitate the detection of microorganisms.

  Furthermore, as shown in FIG. 3, a circular frame layer (20) is formed on a square base sheet (10), a medium layer (30) is formed in the frame layer (20), and at least the medium layer The cover sheet layer (40) may be laminated so as to cover (30). A medium layer can be formed only on the frame layer, and waste of an expensive medium layer can be avoided. 3A is a partially transparent plan view, and FIG. 3B is a cross-sectional view taken along line A-A ′ of FIG. At this time, as shown in the cross-sectional view of FIG. 4, a medium layer (30) is formed on the cover sheet (40) so as to face the medium layer (30) formed on the base sheet (10). May be. Further, as shown in the cross-sectional view of FIG. 5, an adhesive layer (50) is formed on the cover sheet (40) so as to face the frame layer (30) formed on the base sheet (10). Also good. Since the frame layer (20) and the cover sheet (40) are bonded via the adhesive layer (50), the medium layer (30, 30) is more reliably covered with the cover sheet (40). ') Drying and contamination can be prevented more efficiently.

  In the microorganism culture sheet of the present invention, “on the base sheet” means that the base sheet is not directly contacted with the base sheet, but the base sheet is down and the cover sheet is up. The upper part of is also included. Therefore, another layer may be included between the base sheet (10) and the frame layer (20). For example, as shown in the cross-sectional view of FIG. 6, an adhesive layer (50) and a protective layer (60) are sequentially laminated on the base sheet (10), and a frame layer (20) and a culture medium are laminated on the protective layer (60). A layer (30) may be formed. When the culture medium layer (30) is inoculated with the test solution and the culture medium layer (30) is covered with the cover sheet (40), the cover sheet (40) is fixed to the base sheet (10) side by the adhesive layer (50). Thus, drying and contamination of the medium layer (30) can be efficiently prevented. Moreover, since the protective layer (60) is laminated on the lower layer of the culture medium layer (30), it is possible to prevent the sample liquid from leaching into the adhesive layer (50), and at the same time, the culture medium layer of the components contained in the adhesive layer. Elution into (30) can be prevented and microorganisms can be grown. FIG. 6 shows a mode in which a lattice printed layer (70) is further laminated below the base sheet (10).

  The shapes of the base sheet (10) and the frame layer (20) are not limited to the above. For example, the frame is formed so that the square culture medium layer (30) is formed on the square base sheet (10). A layer (20) may be formed. Both the base material sheet (19) and the culture medium layer (30) are rectangular, and a mode in which a plurality of culture medium layers (30) are formed on the base material sheet (10) is shown in FIGS. ). FIG. 7A is a plan view, and FIG. 7B is a cross-sectional view taken along the line B-B ′ of FIG. In FIG. 7, the cover sheet (40) has two corners (C1, C2) so that the cover sheet (40) is fixed to the end of the base sheet (10) and can be in close contact with the frame layer (20). ) Is formed. Although different from FIG. 7, the culture medium layer (30) and the adhesive layer (50) are arranged on the cover sheet (40) so as to face the frame layer (30) formed on the base sheet (10). May be formed.

(2) Substrate sheet The substrate sheet used in the present invention needs to have waterproofness, and preferably has heat resistance to withstand a drying treatment when forming a culture medium layer. For example, polyester, polyethylene Plastic sheets such as polypropylene, polystyrene, polycarbonate, and polyvinyl chloride can be used. The base sheet may be uncolored and transparent, but those exhibiting white color by foaming or those colored white are preferable in terms of easy counting of grown colonies. Other than the plastic sheet, paper subjected to water-resistant processing can also be used. For example, a paper base coated or synthetically coated with a synthetic resin, or a plastic film laminated can be used.

The base sheet is preferably flat without curling, and the thickness is not particularly limited, but is usually 25 to 500 μm, more preferably 50 to 250 μm.
The base sheet may be preprinted with a square print with ink that is insoluble in water and does not affect the growth of microorganisms. This is because counting of colonies can be facilitated by the square print pattern. The printing plate type is not particularly limited and may be anything, but gravure printing or the like is preferable in terms of a wide selection range of colorant, resin, solvent and the like. The size of the mesh is about 1 cm square.

  The microorganism culture sheet of the present invention is for observing and counting colonies grown in the medium layer, and can be observed and counted from the cover sheet side, but may be observed and counted from the base sheet side. In this case, the substrate sheet is preferably transparent.

(3) Medium layer In the present invention, the mass ratio of the water-absorbent resin having a particle diameter of 120 μm or less to the water-soluble polymer (water-absorbent resin / water-soluble polymer) is in the range of 0.05 to 3, and other than water. A culture medium layer is formed by applying a dispersion liquid comprising a polar solvent to the substrate sheet. The water-absorbent resin absorbs 400 to 700 times its own weight of water to form a gel, and therefore, in consideration of the shape after swelling due to water absorption, the water-absorbent resin is uniformly dispersed in the medium layer while ensuring a predetermined interval. Preferably present. Further, once the water-absorbing resin absorbs water, it cannot return to its original shape even if it is subsequently dried, and the water absorption performance is reduced. Considering such characteristics, as a method for uniformly dispersing a water-absorbing gelling agent without dissolving it in water, a method in which an organic solvent is used and dispersed together with a hydrophobic binder is generally used. However, when a hydrophobic binder is included in the culture medium layer of the microorganism culture sheet, the test solution cannot be uniformly diffused into the culture medium layer of the microorganism culture sheet. Therefore, in the present invention, a water-soluble polymer is used, and a polar solvent other than water is used as a solvent in which the water-soluble polymer can be dissolved in order to uniformly disperse the water-absorbing resin without dissolving it in water. The water-absorbent resin was uniformly dispersed. When the obtained dispersion is applied onto a base sheet and the solvent is removed, a medium layer in which particulate water-absorbing resin is uniformly dispersed in the polymer can be obtained.

  Examples of the water-absorbing resin used in the present invention include polyacrylate cross-linked products, polyethylene oxide cross-linked products, acrylate / vinyl alcohol copolymers, carboxymethyl cellulose cross-linked products, and starch / acrylonitrile graft copolymer hydrolysates. And what has water absorption can be used conveniently. In general, a (meth) acrylic acid polymer having an internal cross-linked structure or a surface cross-linked structure can be suitably used.

  The average particle size of the water absorbent resin is 120 μm or less, preferably 5 to 120 μm, more preferably 10 to 80 μm. It may be a powder form in which no particle diameter is recognized. On the other hand, when it exceeds 120 μm, dispersion into the water-soluble polymer becomes difficult, and when the test solution is dropped, the particles may swell and become a large gel, which may hinder the detection of microbial colonies.

  The water-soluble polymer used in the present invention is limited to those that are water-soluble and can be dissolved in polar solvents other than water. Among these, polyvinylpyrrolidone and polyethylene oxide can be preferably used, and cellulose derivatives such as hydroxypropylcellulose can also be preferably used. In particular, polyvinyl pyrrolidone and hydroxypropyl cellulose have an appropriate viscosity as a binder, can uniformly disperse the water-absorbent resin, and are excellent in adhesion to the substrate sheet. This is preferable in that the medium layer can be formed by applying the dispersion without performing surface treatment for the purpose.

  The solvent used in the present invention is a polar solvent other than water that can dissolve the water-soluble polymer, and examples thereof include ethyl alcohol, methyl alcohol, propyl alcohol, and butyl alcohol.

  As for these blending ratios, the mass ratio of the water-absorbent resin to the water-soluble polymer (water-absorbent resin / water-soluble polymer) is 0.05 to 3, more preferably 0.1 to 2.0. If it exceeds 3, the amount of water-absorbing resin is relatively large, and therefore, when the test solution is administered, local water absorption occurs, and a sufficient observation area may not be ensured. On the other hand, if it is less than 0.03, the water absorption is not sufficient, so that the test solution may diffuse in the lateral direction due to the cover sheet covering and leak from the microorganism culture sheet.

  In the culture medium layer of the present invention, nutrient components of microorganisms may be added to the dispersion. As a nutrient component of such microorganisms, for example, for general bacterial testing, yeast extract / peptone / glucose mixture, meat extract / pepton mixture, peptone / soybean peptone / glucose mixture, etc., and dipotassium phosphate and A mixture of sodium chloride is used for testing Escherichia coli / Coliform bacilli. Sodium desoxycholate / peptone / ammonium iron citrate / sodium chloride / dipotassium phosphate / lactose / neutral red, peptone / lactose / phosphate Dipotassium / eosin Y / methylene blue mixture for staphylococci, meat extract / peptone / sodium chloride / mannite / phenol red / yolk mixture, peptone / meat extract / yeast extract / sodium pyruvate / glycine / lithium chloride・ Tellurite eggs Liquid mixtures etc. are for yeast extract, peptone, sucrose, sodium thiosulfate, sodium citrate, sodium cholate, ferric citrate, sodium chloride, beef bile, bromthymol blue, thymol blue, etc. For enterococci, bovine brain extract, heart extract, peptone, glucose, dipotassium phosphate, sodium nitride, bromthymol blue, 2,3,5-triphenyltetrazolium chloride mixture, etc. for fungi, peptone, A glucose mixture, a yeast extract / glucose mixture, a potato extract / glucose mixture and the like are used. One or more can be selected from these according to the microorganism to be grown and mixed for use. Since the nutrient component is supplied as a powder, it can be mixed with a water-absorbing resin or a water-soluble polymer. In addition, it is good also as a culture medium in which microorganisms can grow by adding the water containing the growth nutrient component of microorganisms to the test liquid containing microorganisms.

  Furthermore, a dye that can be metabolized by microorganisms can be added to the medium layer. Such dyes are metabolized by microorganisms that grow during the culturing process, and the colonies are colored, so that they have the effect of facilitating counting of the number of colonies. Specific examples of such dyes include tetrazolim salts such as triphenyltetrazolium chloride (hereinafter referred to as TTC), p-tolyltetrazolium red, tetrazolium violet, and petetryltetrazolium blue, and pH indicators. As the microorganism grows, the color develops or changes color so that the growth of the microorganism becomes easy to see, and the specific microorganism can be detected by adding the color developed or fluorescent enzyme substrate of the enzyme possessed by the particular microorganism.

  Furthermore, a selective agent that suppresses the growth of microorganisms other than the detection purpose may be added, and a color developing or fluorescent enzyme substrate may be added to detect a specific microorganism. As such a selective agent, antibiotics such as antibiotics and synthetic antibacterial agents, dyes, surfactants, inorganic salts and the like are used. Examples of antibiotics include methicillin, ceftamezole, cefixime, ceftazidime, cefsulosin, bacitracin, polymyxin B, rifampicin, novobiocin, colistin, lincomycin, chloramphenicol, tetracycline, streptomycin, etc. Examples thereof include nalidixic acid and olaquindox. Examples of the pigment include bacteriostatic or bactericidal crystal violet, brilliant green, malachite green, and methylene blue. Examples of the surfactant include Tergitol 7, dodecyl sulfate, lauryl sulfate and the like. Examples of inorganic salts include selenite, tellurite, sulfite, sodium nitride, lithium chloride, oxalate, and high-concentration sodium chloride. Besides these, taurocholate, glycine, bile powder, bile salt, deoxycholate, and the like can be used.

  The medium layer can be formed by applying the above dispersion on a base sheet. In the present specification, “coating” refers to an operation of attaching the above dispersion liquid on a base sheet, a method of applying with a brush or a roll, a method of applying on a base sheet by screen printing, etc. Including methods. The coating method can be appropriately selected according to the viscosity of the dispersion, the thickness of the medium layer, and the like. In the present invention, the medium layer can be formed by coating with a roll coater or the like, and the medium layer can be formed into a thin layer by screen printing or the like.

  In this invention, it is not limited to a base material sheet, A culture medium layer can be formed in a cover sheet by the same method. Under the present circumstances, the thickness of the culture medium layer formed on a base material sheet and a cover sheet can be suitably selected by the amount of water absorption of the culture medium base material which comprises a culture medium layer, a water absorption speed, the area of a culture medium layer, etc. In addition, the composition of the culture medium layer formed on the base sheet and the cover sheet may be the same or different, and the thickness may be the same or different.

  In addition, when a frame layer is formed on the base sheet as described later, and a medium layer is formed only inside the frame layer, the frame layer is arranged so as to be equal to the height of the frame layer after absorbing the test solution. It is preferable to install. Although the medium base material is expensive, in the present application, since the medium layer can be formed limited to the inside of the frame layer, it is possible to avoid the formation of an unnecessary range of the medium layer and to eliminate waste. .

(4) Frame layer In this invention, the frame layer which consists of UV foaming ink is formed on a base material sheet, and, thereby, the spread of the test liquid to inoculate can be made constant. In the present invention, the water absorption resin is used for the culture medium layer, so the test liquid is excellent in water absorption, but by further forming a frame layer on the base sheet, the spread range of the test liquid is adjusted to be constant. can do. Specifically, since the frame layer is formed of a hydrophobic UV foaming ink, a predetermined amount of a test solution is dropped on the medium layer surrounded by the frame layer by lifting the cover sheet during culture, and the cover sheet The test solution can be spread evenly over the entire area surrounded by the frame layer without using a special instrument. The medium layer absorbs the moisture of the test solution, swells and gels, the pre-contained nutrients elute, and the microorganisms in the test solution are evenly dispersed and trapped in the gel. Incubation can be performed, and colonies that can be easily counted can be formed by culturing.

  In the microorganism culture sheet of the present invention, the frame layer may be formed on the medium layer formed on the entire surface of the base sheet, but if it is formed directly on the base sheet, the medium layer can be wasted. . In addition to forming directly on the base sheet, as shown in FIG. 6, when the adhesive layer (50) and the protective layer (60) are formed on the base sheet (10), the protective layer (60 ) May form a frame layer (20). Although different from FIG. 6, a frame layer (20) is formed on the adhesive layer (50), and a protective layer (60) and a medium layer (30) are formed inside the frame layer (20). May be.

  In the present invention, the frame layer limits the area of the culture medium layer (30) inoculated with the test liquid to a predetermined range, thereby easily and accurately measuring the number of microorganisms contained in the predetermined liquid volume. sell. Therefore, the width of the frame layer is not limited to a uniform width as long as the above-described object can be achieved, but the most detailed width is preferably 0.5 to 5.0 mm, more preferably 1.0 to 3.0 mm. If it is the said range, even when a culture-medium layer (30) absorbs water, the shape of a culture-medium layer (30) can be maintained and it can function as a division part of the inner side of a frame layer, and an outer side. Moreover, generally the height of a frame layer is 30-500 micrometers, More preferably, it is 50-200 micrometers. If it is less than 30 μm, when the test solution is spread out, it tends to protrude from the frame layer, or the area of the medium layer (30) necessary to absorb the test solution becomes large, making it difficult to count the number of microorganisms There is. On the other hand, if it exceeds 500 μm, a space is generated in the upper part of the medium layer (30) after water absorption, which may make it difficult to count the number of microorganisms with the cover sheet, which is disadvantageous in terms of manufacturing cost. Become. Further, when the shape of the frame layer is a circle, the size of the circle is not limited, but the diameter is preferably 3 to 7 cm.

  The UV foaming ink to be used is provided to keep the spread area of the test solution inoculated at the time of culture in a certain area surrounded by a frame layer. The test solution is water-based and hydrophobic. Ink is used. Accordingly, as the ink used for the frame layer, as long as it is UV foaming, most inks can be used and colored as long as they are not water-soluble inks or hydrophilic inks. What contains the hardening | curing agent can be used conveniently. The UV foamed ink contains one or more ordinary ink vehicles as a main component and contains a UV foaming agent and a curing agent. If necessary, a plasticizer, a stabilizer, an antioxidant, a light stabilizer, an ultraviolet ray Add one or more additives such as absorbents, crosslinking agents, lubricants, antistatic agents, fillers, etc., and add colorants such as dyes and pigments, solvents, diluents It is possible to use an ink composition obtained by sufficiently kneading and the like to prepare an ink composition. As such an ink vehicle, known ones such as sesame oil, drill oil, soybean oil, hydrocarbon oil, rosin, rosin ester, rosin modified resin, shellac, alkyd resin, phenolic resin, maleic resin, Natural resin, hydrocarbon resin, polyvinyl chloride resin, polyacetic acid resin, polystyrene resin, polyvinyl butyral resin, acrylic or methacrylic resin, polyamide resin, polyester resin, polyurethane resin, epoxy resin, urea resin , Melamine resin, amino alkyd resin, nitrocellulose, ethyl cellulose, chlorinated rubber, cyclized rubber, etc. can be used alone or in combination.

  There are various known foaming agents such as an inorganic foaming agent, an organic foaming agent, and a liquid foaming agent. Among them, a foaming agent-containing copolymer, for example, a polymer shell containing a liquid gas is produced. Thermally expandable microcapsules that can be suitably foamed by heat generated during UV irradiation (about 90 to 200 ° C.) can be suitably used. For example, EXPANCEL DU-461 manufactured by Nippon Philite Co., Ltd. Can give. Moreover, it is preferable that a foaming agent mixes in UV foaming ink in the range of about 5-45 mass%. If it is less than 5% by mass, foaming is not sufficient, and if it exceeds 45%, it may be difficult to obtain a printed product in line with the original application, such as an increase in ink viscosity and a decrease in printability.

  In addition, as the UV foaming ink of the present invention, a composition obtained by blending the above UV foaming agent with a composition having a cured coating film physical property containing a reactive diluent, a photopolymerizable oligomer, and a photopolymerization initiator may be suitably used. it can. As the reactive diluent, an acrylate reactive diluent or a methacrylate reactive diluent can be used alone or in combination, and more specifically, 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, 4-hydroxybutyl. Acrylate, phenoxyethyl acrylate, 2-acryloyloxyethyl-succinic acid, dimethylaminoethyl acrylate, ECH modified phenoxy acrylate, lauryl acrylate, lauryl methacrylate, benzyl methacrylate, butoxydiethylene glycol methacrylate, acryloylmorpholine, glycerin monomethacrylate, 1,9 -Nonanediol diacrylate, tripropylene glycol diacrylate, neopentyl glycol diacrylate, 1 A combination of one or more of 6-hexanediol diacrylate, polyethylene glycol diacrylate, ethylene glycol dimethacrylate, 1,6-ethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, and trimethylolpropane triacrylate Can be used. As the photopolymerizable oligomer, one or two of urethane acrylate photopolymerizable oligomer, polyester acrylate photopolymerizable oligomer, epoxy acrylate photopolymerizable oligomer, acrylic photopolymerizable oligomer, and special photopolymerizable oligomer A combination of the above can be used. Moreover, as a photoinitiator, if it is a commercially available photoinitiator normally used for ultraviolet curable ink, it will not specifically limit. For example, 2-hydroxy-2-methyl-1-phenyl-propan-1-one, 1-hydroxy-cyclohexyl-phenyl-ketone, and the like can be used. The case of coloring the ultraviolet curable ink is not particularly limited, but in this case, it is preferable to use 2,4,6-trimethylbenzoyl-diphenyl-phosphine oxide or the like. An antifoaming agent, a polymerization inhibitor, a pigment dispersant and the like may be added to the UV foamed ink as additives. Moreover, since the coloring pigment used normally may be contained in the range of less than 15 mass% and exceeds 15 mass%, since there exists a possibility of inhibiting foaming, it is unpreferable.

In addition to screen printing, printing methods such as gravure printing, letterpress printing, transfer printing, flexographic printing, and the like may be used.
Foaming due to UV irradiation varies depending on the type of foaming agent and the like, and can be appropriately selected. However, as an ultraviolet (UV) irradiation condition, a UV lamp of 80 to 82 W / cm and a line speed of 15 to 25 M / min. Illustrates irradiation with a dose of. As the ultraviolet irradiation lamp, a metal halide lamp, a mercury lamp, or the like can be used.

  In the present invention, if the frame layer is screen-printed on the substrate sheet using the UV foaming ink and then irradiated with ultraviolet rays under the above conditions, the foaming agent foams as the ink is dried. As a result, a foamed printing layer (ultraviolet curable coating film) is formed on the surface of the base material to form a thick frame layer.

In the present invention, since UV foamed ink is used, it can be instantly cured by UV irradiation.
(5) Cover sheet In this invention, a cover sheet has the effect | action which prevents the evaporation by the water | moisture content of a culture medium layer while preventing the contamination by the falling bacteria etc. during culture | cultivation. The cover sheet is waterproof and water vapor impermeable, and at the same time is preferably transparent so that colonies can be observed and counted through the cover sheet after culturing microorganisms, for example, as mentioned in the base sheet. Polyolefins such as polyethylene and polypropylene, and plastic films such as polyester, polyamide, polystyrene, polycarbonate, and polyvinyl chloride can be used. Furthermore, when the cover sheet is lifted from the base sheet in order to inoculate the test liquid, it is preferable that moderate flexibility is secured. Therefore, the thickness of the cover sheet is preferably about 10 to 200 μm, and more preferably about 20 to 70 μm. In view of workability such as opening and closing of the cover sheet, a polyester film, a polyolefin film or an easily peelable polyolefin film is particularly preferable. The cover sheet may have any shape, but it is necessary to have a size larger than the medium layer so that the medium layer can be covered in order to prevent invasion of various bacteria. Furthermore, when a culture medium layer (30 ′) or an adhesive layer is formed on the cover sheet, they need to be appropriately bonded, and in order to ensure the adhesion of each layer, a corona discharge treatment or the like is applied to the coated surface of the cover sheet. Can be pre-processed. The cover sheet preferably has suitable gas permeability, mainly oxygen permeability, depending on the type of microorganism to be cultivated, and is selected in consideration of this point.

  In the present invention, the cover sheet only needs to be able to cover the medium layer, and is not limited to the case where the cover sheet is fixed to the microorganism culture sheet. Therefore, the medium layer inoculated with the test solution may be covered with a separately easy cover sheet, and as shown in FIG. 7, the cover sheet is previously fixed to the base sheet. Also good.

(6) Adhesive layer In this invention, an adhesive layer can be formed in the outer side etc. of the frame layer of a base material sheet. Such an adhesive is used for fixing the cover sheet coated with the culture medium layer and for the purpose of preventing evaporation and contamination of the culture medium layer, and any kind of material can be used as long as the base sheet can be sealed. Considering workability, a pressure-sensitive adhesive having a weak adhesive strength is preferable to the extent that the pressure-sensitive adhesive sheet and the transparent film can be re-adhered. For example, a rubber adhesive and an acrylic adhesive are preferable, and a re-peeling type and a slightly adhesive type acrylic adhesive are particularly preferably used. Specifically, 2 to 15 parts by mass of a monomer containing a functional group such as a carboxyl group, a hydroxyl group or an amino group is added to 100 parts by mass of an acrylate having 2 to 12 carbon atoms such as butyl acrylate or 2-ethylhexyl acrylate. A polymer obtained by adding rosin, xylene resin, phenol resin or the like as a tackifier to the polymerized polymer and crosslinking with melamines, isocyanates, epoxy, or the like can be used. Further, the pressure-sensitive adhesive layer may have any shape, but it is preferable that the pressure-sensitive adhesive layer is disposed so that there is no gap between the pressure-sensitive adhesive sheet and the transparent film.

  In the present invention, the cover sheet and the base sheet are adhered to each other with the adhesive layer. However, the medium is not limited to the adhesive layer as long as the medium layer can be sealed with the cover sheet. It may be.

(7) Protective layer When an adhesive layer is formed on a base material sheet, it is preferable to form a protective layer on the adhesive layer and form a culture medium layer on the protective layer. This is for preventing the test liquid from being absorbed into the adhesive layer after the medium layer has absorbed the test liquid and preventing the growth of microorganisms from being inhibited by the components contained in the adhesive layer.

  Therefore, as the protective layer, a material having waterproofness and water vapor impermeability can be suitably used as in the case of the substrate sheet and the cover sheet. If the protective layer is transparent, colonies can be observed and counted from the base sheet side.

  The protective layer may be formed by laminating a previously formed film or sheet on the adhesive layer, or may be formed by applying a resin capable of forming the base sheet or cover sheet. The thickness of the protective layer is not particularly limited as long as the above object can be achieved, and generally 25 to 100 μm is preferable.

(8) Lattice Printed Layer A lattice printed layer may be formed on the microorganism culture sheet used in the present invention. Such a lattice printing layer may be an embodiment in which a resin film or a paper substrate on which a grid is printed is laminated on the lower layer of the substrate sheet, but the substrate film is subjected to lattice printing, and the lattice printing layer May be formed. In addition, lattice printing may be formed on the cover sheet.

(9) Production method The microorganism culture sheet of the present invention is a microorganism culture sheet comprising a substrate sheet, a medium layer formed on the substrate sheet, and a cover sheet covering the medium layer, The medium layer has a mass ratio of a water-absorbing resin having a particle size of 120 μm or less to a water-soluble polymer (water-absorbing resin / water-soluble polymer) in the range of 0.05 to 3, and is composed of a polar solvent other than water. It is formed by applying a dispersion. It is preferable that the medium layer is free of bubbles and the water-absorbing resin is uniformly dispersed. In the present invention, as described above, a water-soluble polymer that can be dissolved in a polar solvent such as polyvinylpyrrolidone is used after being dissolved in a polar solvent such as alcohol. Polyvinylpyrrolidone is excellent in water absorption, does not generate bubbles when dissolved in a polar solvent, and does not increase in the viscosity of the solution even when dissolved at a high concentration, making it easy to perform coating and drying operations. be able to. A dispersion can be prepared by dispersing a water-absorbing resin in this water-soluble polymer solution. The dispersion used in the present invention can improve adhesion to a base sheet or cover sheet by blending a water-soluble polymer, so that the surface treatment is not performed on the base sheet or cover sheet. A medium layer can be formed by applying the dispersion.

In the present invention, when the culture medium dispersion is applied, it can be applied by a known method such as roll coating, bar coating, air knife coating, comma coating, or gravure coating. The medium layer is applied such that the coating amount when dried is 3 to 20 g / m ² , more preferably 5 to 15 g / m ² . If it is less than 3 g / m ² , sufficient water absorption may not be ensured. On the other hand, if it exceeds 20 g / m ² , the drying time becomes longer and the productivity may be lowered. Although depending on the drying capacity of the coating apparatus, when coating cannot be performed once by coating, the coating may be performed in several times. The advantages of the coating method are that the coating amount can be stabilized and that the productivity is low with little material loss. Subsequently, the medium layer can be formed by heating and drying.

  In forming the medium layer, the medium dispersion liquid may be printed on the base sheet or the cover sheet. As a printing method, in addition to screen printing, printing methods such as gravure printing, letterpress printing, transfer printing, flexographic printing, and the like can be used.

  When the microorganism culture sheet of the present invention is formed by laminating an adhesive layer, a protective layer and a culture medium layer on the base material sheet, and forming a frame layer on the culture medium layer, the adhesive layer, the protective layer and the culture medium layer are formed on the base material sheet. The protective layer may be formed in a predetermined range of the base sheet on which the adhesive layer has been formed in advance, and then the medium layer may be applied, and then the frame layer may be printed. As a printing method for the frame layer, in addition to screen printing, printing methods such as gravure printing, letterpress printing, transfer printing, flexographic printing, and the like may be used.

  Moreover, when forming the frame layer which consists of UV foaming ink on a base material sheet, for example, (i) On the base material sheet, a dispersion liquid is applied in a predetermined shape, and the dispersion liquid is dried to form a medium layer (Ii) a step of printing a frame mold with UV foaming ink on the outer periphery of the medium layer, and (iii) a step of foaming the foamed ink by UV irradiation to form a frame layer. be able to. In addition, when the medium layer is formed in the pre-formed frame layer, (iv) a step of printing a frame mold with UV foaming ink on the substrate sheet, (v) foaming the foamed ink by UV irradiation And (vi) applying a dispersion liquid in the frame layer and drying the dispersion liquid to form a medium layer. In this case, the step of irradiating the UV foamed ink with UV may be before or after the formation of the medium layer. Therefore, following the step (iv), (v) applying the dispersion into the frame mold, You may perform the process of drying a dispersion liquid and forming a culture medium layer, and performing the said (v) process after that. In the case where the microorganism culture sheet of the present invention has a cover sheet fixed thereto, (iv), (v) is followed by (vii) a step of fixing the cover sheet to the substrate sheet, and thereafter The frame layer may be formed by irradiating the frame mold with UV from the upper part of the cover sheet to foam the foamed ink.

  In the present invention, in order to apply the dispersion in a predetermined shape, the dispersion can be formed by printing on a substrate sheet. As a printing method, in addition to screen printing, printing methods such as gravure printing, letterpress printing, transfer printing, flexographic printing, and the like may be used. Subsequently, the medium layer can be formed by heating and drying.

  Moreover, the culture medium layer (30) of a predetermined shape can be provided on the cover sheet (40) together with the base sheet (10) by the same method. The amount of the dispersion applied may be the same as that for forming the medium layer on the base sheet.

  In addition, when forming a dispersion liquid by printing in the case of formation of a frame layer and a culture medium layer, both a frame layer and a culture medium layer can be formed by printing. Printing of the frame layer and the culture medium layer may be performed at the same time, or one of them may be performed first. When the medium layer is formed by screen printing, it is preferable to print the medium base material first to form the medium layer, and then form the frame layer.

  When the microorganism culture sheet of the present invention is such that an adhesive layer and a protective layer are laminated on a base sheet, a frame layer is formed on the adhesive layer or the protective layer, and a culture medium layer is formed on the protective layer May be formed in the order of an adhesive layer, a protective layer, a medium layer, and a frame layer on the base sheet, and a protective layer is formed in a predetermined range of the base sheet on which the adhesive layer has been formed in advance, The layers may be formed sequentially. Such an adhesive layer can be formed by a printing method. As a printing method, in addition to screen printing, printing methods such as gravure printing, letterpress printing, transfer printing, flexographic printing, and the like may be used.

  In the microorganism culture sheet of the present invention, the medium layer is inoculated with the cover sheet after the test solution is inoculated, and the cover sheet is cultured, so that a part of the cover sheet is fixed to the base sheet. It is simple in operation. However, in the case of a direct stamp or a wiping test, when the work efficiency is improved by the absence of the cover sheet, the cover sheet may be removed and used.

  When the cover sheet and the base sheet are composed of the same member, the cover sheet prepared separately may be fixed to the base sheet by heat sealing, laminating, or the like. The same material is used for the sheet, and the base sheet (10) and the cover sheet (40) are connected continuously as shown in the plan view of FIG. 8 (a) and the sectional view of FIG. 8 (b). A frame layer (20) and a medium layer (30) may be formed on the material sheet (10), and the cover sheet (40) may be bent to form a microorganism culture sheet. FIG. 8C shows a sectional view thereof. According to this method, even when the medium layer (30 ′) or the adhesive layer (50) is formed on the cover sheet, the base sheet (10) and the cover sheet (40) are continuously provided. The medium layer (30 ′) can be formed accurately and simply at a position facing the medium layer (30) above (10).

The microorganism culture sheet of the present invention is sterilized by gamma ray irradiation or ethylene oxide gas sterilization to obtain a product of the microorganism culture sheet of the present invention.
The microorganism culture sheet of the present invention can cultivate various microorganisms according to the type of microbial nutrients contained, the base sheet used, the air permeability of the cover sheet, and others.

EXAMPLES Next, although an Example is given and this invention is demonstrated concretely, these Examples do not restrict | limit this invention at all.
Example 1
20 parts by mass of polyvinylpyrrolidone (made by Nippon Shokubai Co., Ltd., trade name “PVP K-90”, K value 88 to 96) is dissolved in 100 parts by mass of methanol, and a water-absorbent resin (manufactured by Sanyo Chemical Industries, Ltd.) is dissolved in this solution. , Trade name “500 MPSA”: 8 parts by mass of a center particle diameter of 20 to 50 μm, water absorption of 400 g / g (deionized water) was uniformly dispersed to obtain a dispersion.

This dispersion was screen-printed on a transparent polyester film having a thickness of 100 μm (E5100 manufactured by Toyobo Co., Ltd.) so that the coating amount after drying was 7.9 g / m ² . Cut to a side of 75 mm.

On the other hand, a 25 μm-thick polypropylene film (trade name “OP U-1” manufactured by Tosello Co., Ltd.) was used as the cover sheet, and the dispersion was coated so that the coating amount after drying was 7.9 g / m ^2. A medium layer was formed by screen printing, and after drying, the medium layer was cut into 95 mm length and 75 mm width.

The substrate sheet and the cover sheet were each sterilized with γ rays to obtain the microorganism culture sheet of Example 1.
Pipette 1 ml of purified water onto the culture medium layer of the microorganism culture sheet, place the cover sheet on top of the medium layer with the medium layer side down, and gently press the test sheet over the medium layer. The gel was allowed to stand for about 1 minute to form a gel, and the presence or absence of liquid leakage from the end of the microorganism culture sheet was evaluated. The results are shown in Table 1.

(Examples 2 and 3)
A microorganism culture sheet was prepared in the same manner as in Example 1 except that the composition of the dispersion was changed to the composition shown in Table 1, and the presence or absence of liquid leakage was evaluated in the same manner as in Example 1. The results are shown in Table 1.

In Table 1, PVP K-30 is made by Nippon Shokubai Co., Ltd., polyvinyl pyrrolidone, K value 27-33.
(Comparative Examples 1 and 2)
A microorganism culture sheet was prepared in the same manner as in Example 1 except that the composition of the dispersion was changed to the composition shown in Table 1, and the presence or absence of liquid leakage was evaluated in the same manner as in Example 1. The results are shown in Table 1.

  In Table 1, 500D is a water-absorbent resin (manufactured by Sanyo Chemical Industries, Ltd., center particle diameter 150 to 710 μm, water absorption 400 g / g (deionized water)).

（実施例４）
菌株として大腸菌を使用し、液体培地（NUTRIENT BROTH）にて３７℃で２４時間振盪培養を行った菌液を滅菌水で菌数が１０²／ｍｌとなるように希釈して、培養試験に用いる菌液を作成した。上記の菌液を、実施例１の微生物培養シートの培地層の上に滅菌済みのピペットで各１ｍｌ接種し、その上にカバーシートを培地層を設けた面を下にして乗せ、その上から軽く押さえて被検液を培地層全体に広げ、約１分間静置してゲルを形成させた後、孵卵器に入れ、３７℃で４８時間培養した。

Example 4
Escherichia coli is used as a strain, and a bacterial solution obtained by shaking culture at 37 ° C. for 24 hours in a liquid medium (NUTRIENT BROTH) is diluted with sterilized water so that the number of bacteria becomes 10 ² / ml and used for a culture test. A bacterial solution was prepared. Inoculate each 1 ml of the above bacterial solution with a sterilized pipette on the culture medium layer of the microorganism culture sheet of Example 1, and place the cover sheet thereon with the surface provided with the culture medium layer facing down. The test solution was spread lightly over the entire culture medium layer and allowed to stand for about 1 minute to form a gel, which was then placed in an incubator and cultured at 37 ° C. for 48 hours.

The number of colonies generated after the culture was counted. Five culture samples were prepared. The results are shown in Table 2.
(Comparative Example 3)
A culture sample for comparison was prepared by mixing 1 ml of the bacterial solution of Example 4 with 20 ml of a standard agar medium prepared in a Petri dish by a conventional pour method. This culture sample was placed in an incubator and cultured at 37 ° C. for 48 hours in the same manner as in the Examples.

  The number of colonies generated after the culture was counted. Five culture samples were prepared. The results are shown in Table 2.

  The microorganism culture sheet of the present invention can be used for testing the number of bacteria and has excellent water absorption, so that liquid leakage from the end can be prevented, and the operation is simple and useful.

It is the top view (a) and sectional drawing (b) which show an example of the microorganisms culture sheet which consists of a base material sheet, a culture medium layer, and a cover sheet based on this invention. It is a microorganism culture sheet which consists of a base material sheet, a culture medium layer, and a cover sheet based on this invention, Comprising: It is sectional drawing of the aspect by which the culture medium layer was formed in the cover sheet. It is the top view (a) and sectional drawing (b) which show an example of the microorganisms culture sheet which consists of a base material sheet, a frame layer, a culture medium layer, and a cover sheet based on this invention. It is a microorganism culture sheet which consists of a base material sheet, a frame layer, a culture medium layer, and a cover sheet based on this invention, Comprising: It is sectional drawing of the aspect by which the culture medium layer was formed in the cover sheet. It is a microorganism culture sheet which consists of a base material sheet, a frame layer, a culture medium layer, and a cover sheet based on this invention, Comprising: It is sectional drawing of the aspect by which the culture medium layer and the adhesion layer were formed in the cover sheet. It is a microorganism culture sheet which consists of a base material sheet, a frame layer, a culture medium layer, and a cover sheet based on this invention, Comprising: It is sectional drawing of the aspect by which an adhesion layer and a protective layer are formed in a base material sheet. The top view (a) and cross section which are the microorganism culture sheets which consist of a base material sheet, a frame layer, a culture medium layer, and a cover sheet based on this invention, Comprising: A several culture medium layer is formed on a base material sheet FIG. It is a figure explaining the manufacturing method of the microorganisms culture sheet which consists of a base material sheet, a frame layer, a culture medium layer, and a cover sheet concerning the present invention, Comprising: It is a top view (a) and sectional drawing (b), (c) which show the aspect which forms a frame layer and a culture medium layer on top and bends a cover sheet to form a microorganism culture sheet.

Explanation of symbols

10 ... base material sheet,
20 ... Frame layer,
30 ... medium layer,
40 ... cover sheet,
50: Adhesive layer,
60 ... Protective layer,
70 ... Lattice printing layer

Claims (8)

A microorganism culture sheet comprising a substrate sheet, a medium layer formed on the substrate sheet, and a cover sheet covering the medium layer,
The medium layer has a mass ratio of a water-absorbing resin having a particle size of 120 μm or less to a water-soluble polymer (water-absorbing resin / water-soluble polymer) in the range of 0.05 to 3, and from a polar solvent other than water. A microorganism culture sheet, which is formed by applying the dispersion liquid to the substrate sheet.   The microbial culture sheet according to claim 1, wherein the water-soluble polymer is at least one selected from the group consisting of polyvinylpyrrolidone, polyethylene oxide, and cellulose derivatives.   The microorganism culture sheet according to claim 1 or 2, wherein the polar solvent is at least one selected from the group consisting of ethyl alcohol, methyl alcohol, propyl alcohol, and butyl alcohol. A frame layer made of UV foam ink is formed on the base sheet,
The microorganism culture sheet according to any one of claims 1 to 3, wherein the medium layer is formed in the frame layer.   The said cover sheet | seat is formed in the surface facing the culture medium layer formed on the said base material sheet, The culture medium layer formed by apply | coating the said dispersion liquid is formed, The characterized by the above-mentioned. The microorganism culture sheet in any one.   The microorganism culture sheet according to claim 1, wherein the cover sheet is fixed to the base sheet. A method for producing a microorganism culture sheet comprising a substrate sheet, a medium layer formed on the substrate sheet, and a cover sheet covering the medium layer,
On the base sheet, the mass ratio of the water-absorbent resin having a particle diameter of 120 μm or less to the water-soluble polymer (water-absorbent resin / water-soluble polymer) is in the range of 0.05 to 3, and a polar solvent other than water A step of applying a dispersion liquid comprising:
Next, the method for producing a microorganism culture sheet, comprising the step of removing the polar solvent contained in the dispersion. A microorganism culture sheet comprising a base material sheet, a culture medium layer formed on the base material sheet, and a cover sheet covering the culture medium layer, the frame made of UV foaming ink on the base material sheet A method for producing a microorganism culture sheet, wherein a layer is formed and the medium layer is formed in the frame layer,
On the inner side of the frame layer, the mass ratio of the water-absorbent resin having a particle diameter of 120 μm or less to the water-soluble polymer (water-absorbent resin / water-soluble polymer) is in the range of 0.05 to 3, and a polar solvent other than water Applying a dispersion consisting of:
Removing the polar solvent contained in the dispersion;
Forming a frame layer made of UV foamed ink on the base sheet;
A method for producing a microorganism culture sheet, comprising a step of foaming the UV foamed ink by irradiating UV.

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