JP2009171988A - Bio sensor chip - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a bio sensor chip where the amount of a sample to be collected and required for measurement is decreased to reduce the labor of the user and where a sample at a puncture opening is easily collected and measured. <P>SOLUTION: The bio sensor chip has a chip body 11 which has at its forward end 11a a sample collection opening 12a for collecting a sample B, and a slider 20 which has a piercing instrument 21 at its forward end 20a and is slidable along the chip body 11 toward the forward end of the chip body 11. That is, since the slider 20 having the piercing instrument 21 at the forward end 20a is slid along the chip body 11 of the bio sensor chip 10 toward its forward end where the sample collection opening 12a is provided, a subject can be easily pierced, and the sample, such as blood B, flowing out of the puncture opening can be reliably collected from the sample collection opening 12a. Also, the bio sensor chip does not erroneously hurt the user during use because the piercing instrument 21 is not projected from the chip body 11 before piercing and after piercing. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a biosensor chip for measuring and analyzing chemical substances.

Conventionally, for example, a biosensor chip that detects the concentration of glucose in blood is known (see, for example, Patent Document 1).
FIG. 6 is an exploded perspective view showing the glucose sensor described in Patent Document 1. As shown in FIG. As shown in FIG. 6, a glucose sensor 100 that is a biosensor has a counter electrode 101 and a working electrode 102. The counter electrode 101 has a hollow needle shape half cut in the length direction, and a tip portion 103 thereof is obliquely cut into an injection needle shape so as to be easily punctured. The cut surfaces that have been cut are generally coated with insulating layers 104 and 104 'that also serve as adhesive layers, such as epoxy resin adhesives, silicone adhesives, or glass. The insulating layers 104 and 104' The working electrode 102 is attached via The working electrode 102 is a flat plate member on which glucose oxidase (GOD) is immobilized, and is adhered to the counter electrode 101 with the surface on which the GOD is immobilized facing inward.
Therefore, blood is collected by puncturing the subject 103 with the tip 103 of the needle-shaped counter electrode 101, and the reaction between the collected blood and the immobilized GOD 105 is detected by the working electrode 102 to determine glucose.

In addition, a biosensor in which a biosensor chip and a lancet are integrated is disclosed (see, for example, Patent Document 2).
FIG. 7A is a perspective view of the sensor described in Patent Document 2, and FIG. 7B is an exploded perspective view of the sensor. As shown in FIG. 7, the lancet-integrated sensor 110 includes a chip body 111, a lancet 113, and a protective cover 115. The chip body 111 has a cover 111a and a substrate 111b that can be opened and closed, and an internal space 112 is formed on the inner surface of the cover 111a. The internal space 112 has a shape that can accommodate the lancet 113 in a movable manner.

  The needle 114 provided at the tip of the lancet 113 can be projected and retracted from an opening 112 a formed at the front end of the internal space 112 of the chip body 111 as the lancet 113 moves. The shape of the internal space 111a is curved so that the width thereof is slightly narrower than that of the lancet 113 at the end where the protrusion 113a is located, and the lancet 113 is locked to the chip body 111 by mutual pressing force and frictional force. It is like that. The protective cover 115 has a tube portion 115 a into which the needle 114 is inserted, and the tube portion 115 a can be accommodated inside the chip body 111 as the needle 114 moves. Therefore, in a state before use, the protective cover 115 is put on the needle 114 to protect the needle 114 and prevent the user from being accidentally injured. Note that the substrate 111b is provided with a pair of electrode terminals 116 so that the substrate 111b can be electrically connected to a measuring apparatus (not shown).

In use, the protective cover 115 is removed and the lancet 113 is pushed to cause the needle 114 to protrude from the tip body 111. After the subject is punctured in this state, the needle 114 is housed inside the chip body 111, the opening 112a provided at the front end of the chip body 111 is brought close to the puncture port, and the outflowed blood is collected.
JP-A-2-120655 International Publication No. 02-056769 Pamphlet

However, in the glucose sensor 100 described in Patent Document 1, since the needle-like counter electrode 101 and the working electrode 102 are bonded together, the diameter of the puncture needle is approximately the same as the width of the glucose sensor 100 and increases. For this reason, there is a problem that the amount of blood collected increases and the pain during puncture increases, increasing the burden on the user.
In addition, the lancet-integrated sensor 110 described in Patent Document 2 has a structure that absorbs blood flowing out from the puncture port from the opening 112a, but the structure is complicated.

  The present invention has been made in view of the above-described problems, and its purpose is to reduce the burden on the user by reducing the amount of sample collected for measurement and to easily collect a sample of the puncture port. An object of the present invention is to provide a biosensor chip that can be measured.

In order to achieve the above-described object, a first feature of the biosensor chip according to the present invention is that a chip body having a sample collection port for collecting a sample at one end, and a puncture device at one end are provided in the chip body. And a slider slidable toward the tip of the chip body.
In the present invention, a needle, a lancet needle, a cannula and the like are referred to as a puncture device.

  In the biosensor chip configured in this way, the slider having a puncture instrument at one end slides toward one end where the sample collection port is provided along the chip body of the biosensor chip. A sample such as blood flowing out from the puncture port can be reliably collected from the sample collection port without positioning the sample collection port at the puncture port. For this reason, analysis can be performed with a small amount of sample, and the burden on the subject can be reduced. In addition, since the puncture device does not protrude from the chip body before and after puncturing, it is possible to prevent a user or the like from being injured accidentally during handling.

  Further, a second feature of the biosensor chip according to the present invention is that, in the first feature of the present invention, the sample collection port is located in the vicinity of a puncture position by the puncture device.

  In the biosensor chip configured as described above, the sample collection port provided at the tip of the chip body is located in the vicinity of the puncture port that is punctured by the puncture device of the slider. Puncture and sample collection can be performed, and after puncturing by the puncture device, the sample flowing out to the puncture port can be collected easily and reliably without positioning the sample collection port at the puncture port. For this reason, even a user with reduced visual acuity can easily measure, and the burden can be reduced.

  A third feature of the biosensor chip according to the present invention is that the slider and the chip are fitted in the first or second feature of the present invention.

  In the biosensor chip configured as described above, when the slide and the chip are fitted, the needle can be smoothly slid and punctured at a predetermined position.

  According to a fourth aspect of the present invention, in the biosensor system, the biosensor chip according to any one of the first to third aspects of the present invention is connected to the detection electrode of the biosensor chip. And a measuring instrument for analyzing the collected sample.

  In the biosensor system configured as described above, since the slider having a puncture device at the tip slides toward the tip provided with the sample collection port along the chip body of the biosensor chip, the target can be easily obtained. It is possible to puncture a person and, after puncturing, the sample collection port can be reliably collected from the sample collection port without positioning the sample collection port at the puncture port. Can be reduced. In addition, in the state before and after puncturing, the puncture device does not protrude from the chip body, so that it is possible to prevent a user or the like from being accidentally damaged during handling.

  According to the present invention, the slider having the puncture device at the tip slides toward the tip provided with the sample collection port along the chip body of the biosensor chip, so that the subject can be punctured easily. In addition, after puncturing, a sample such as blood flowing out from the puncture port can be reliably collected from the sample collection port without positioning the sample collection port at the puncture port. For this reason, analysis can be performed with a small amount of sample, and the burden on the subject can be reduced. In addition, since the puncture device does not protrude from the chip body in the state before puncture, it is possible to prevent the user or the like from being accidentally damaged during handling.

Hereinafter, a first embodiment according to the present invention will be described in detail with reference to the drawings.
FIG. 1 is a perspective view showing a first embodiment of a biosensor chip according to the present invention, FIG. 2A is a plan view of the biosensor chip, FIG. 2B is a front view of the biosensor chip, and FIG. 4 is a plan view showing an embodiment of a biosensor system according to the invention, and FIGS. 4A to 4C are explanatory views showing an operation of measuring a blood glucose level using the biosensor system. FIG.

  As shown in FIGS. 1 and 2, the biosensor chip 10 according to the first embodiment of the present invention includes a chip body 11 having a sample collection port 12a for collecting a sample at one end 11a, and a puncture device 21 at one end 20a. And a slider 20 slidable toward the one end 11 a of the chip body 11 along the chip body 11.

  The chip body 11 has a substrate 15 that is a flat plate member having a rectangular cross section, and an upper surface 11b of the substrate 15 has a groove-shaped hollow reaction portion 12 having a rectangular cross section, for example. The hollow reaction part 12 is always provided obliquely with respect to the chip body 11, and the tip of the hollow reaction part 12 is exposed to the tip surface 11 a at a corner near the puncture device 21 of the chip body 11 to be sampled. 12a is formed. Thereby, the sample collection port 12a can be provided in the vicinity of the puncture position by the puncture device 21, and after the puncture by the puncture device 21, the sample can be collected without positioning again.

  A pair of detection electrodes 13a and 13b are arranged in parallel at a predetermined interval on the upper surface 11b. The chip body 11 has a cover layer 14, and a spacer layer 25 exists between the cover layer 14 and the electrodes 13 a and 13 b. The spacer layer 25 is cut off, and the hollow reaction part 12 is formed obliquely with respect to the electrode. In the hollow reaction part 12, the detection electrodes 13a and 13b are exposed, and a reagent (not shown) for reacting the collected sample is provided immediately above or in the vicinity of the detection electrode 13.

 As the material of the substrate 15 and the cover layer 14, an insulating material film is selected. As the insulating material, ceramics, glass, paper, biodegradable material (for example, polylactic acid microorganism-producing polyester), polyvinyl chloride, and the like. Examples thereof include thermoplastic materials such as polypropylene, polystyrene, polycarbonate, acrylic resin, polybutylene terephthalate and polyethylene terephthalate (PET), thermosetting resins such as epoxy resins, and plastic materials such as UV curable resins. A plastic material such as polyethylene terephthalate is preferable because of its mechanical strength, flexibility, and ease of chip fabrication and processing. Representative PET resins include Melinex and Tetron (trade names, manufactured by Teijin DuPont Films, Inc.), Lumirror (trade names, manufactured by Toray Industries, Inc.), and the like. The same material can be exemplified as the material of the spacer layer 25.

  The slider 20 has a partially cylindrical curved portion 24, and an upper member 22 and a lower member 23 (see FIG. 2B) are provided at the front end portion and the rear end portion of the curved portion 24, respectively. The section is U-shaped. The chip body 11 is sandwiched between the upper member 22 and the lower member 23 so as to be relatively movable. The slider 20 can move along the chip body 11 without dropping off. A puncture device 21 is fixed to the inner surface of the curved portion 24 of the slider 20, and the puncture device 21 projects forward (from the left front side in FIG. 1) from the tip 20a of the slider 20. Therefore, when the slider 20 is moved forward, the tip of the puncture device 21 protrudes from the tip 11 a of the chip body 11.

  Examples of the material of the slider 20 are the same as those of the substrate 15 and the cover layer 14.

  In consideration of operating with one hand, the puncture device 21 is preferably a lancet needle. That is, the slider 20 is moved by the driving means. By using a lancet needle, it is possible to puncture quickly and relieve pain during puncturing. Although it does not specifically limit as a drive means, For example, an automatic motor, a spring, etc. are mentioned. At this time, it is desirable that the puncture device 21 is covered with resin so that it can be easily slid along the groove 14.

Next, the biosensor system according to the present invention will be described. FIG. 3 shows a configuration of a biosensor system 30 using the biosensor chip 10 described above.
As shown in FIG. 3, the biosensor system 30 includes the above-described biosensor chip 10 and a measuring device 31 that obtains information on a sample collected by connecting to the detection electrodes 13 a and 13 b of the biosensor chip 10. Have. Note that the configuration of the biosensor chip 10 is as described above, and the same reference numerals are given to the portions common to the biosensor chip 10 described above, and the description thereof is omitted here.

  The measuring device 31 includes a power supply 32, a control device 33, a terminal insertion unit 34, and a display unit 35, which are connected to each other. The rear end portion 11c of the chip body 11 of the biosensor chip 10 is inserted and fixed to the terminal insertion portion 34, and the detection electrodes 13a and 13b exposed at the rear end portion 11c of the chip body 11 are electrically connected. To be connected to. The biosensor system 30 is small in size, for example, a handy type that can be held by a person to be inspected with one hand.

Next, with reference to FIGS. 4A to 4C, the usage method will be described by taking as an example a case where a blood glucose level is measured using the biosensor system 30.
First, the rear end portion 11c of the main body 11 of the biosensor chip 10 is inserted and fixed in the terminal insertion portion 34 of the measuring instrument 31, and is electrically connected. The power source 32 of the biosensor system 30 is turned on and it is confirmed whether or not it is normally activated. The biosensor system 30 is held, the protective cap 36 is pressed against the subject, the puncture site is congested, and the tip 11a of the biosensor chip 10 is brought into contact with the blood collection site of the subject M (see FIG. 4A). The slider 20 is moved and puncture is performed with the puncture device 21 (see FIG. 4B).

  As shown in FIG. 4C, when the puncture device 21 is pulled out from the subject M so that the tip 11a of the tip body 11 does not move away from the subject M, the blood B that has flowed out to the puncture port becomes blood B The surface tension and the capillary action of the hollow reaction part 12 are collected and introduced into the hollow reaction part 12. At this time, since the sample collection port 12a is located in the vicinity of the puncture port formed by the puncture device 21, the blood B can be collected easily and reliably without moving the biosensor chip 10. For this reason, it can be used even by the subject M with reduced visual acuity, and can be measured with a small amount of blood, so the burden on the subject at the time of blood collection can be reduced.

  When a predetermined amount of blood is collected, the biosensor system 30 is separated from the subject M, and the measurement result is displayed on the display unit 35. The blood B introduced into the hollow reaction unit 12 reacts with the reagent, and the current value or the charge value (charge amount) data measured by the detection electrodes 13 a and 13 b is sent to the control device 33. A calibration curve data table is stored in the control device 33, and the blood sugar level is calculated based on the measured current value (charge value). When the calculation is completed, the measurement result is displayed on the display unit 35. For example, the blood glucose level can be expressed as a numerical value. Finally, the biosensor chip 10 is removed from the measuring instrument 31. At this time, the slider 20 is returned to its original position so that the puncture device 21 does not protrude from the tip 11a of the chip body 11. Thereby, the user can appropriately dispose of the used biosensor chip 10 without being damaged by the puncture device 21.

In consideration of the blood collection burden of the subject to be examined, the volume of the hollow reaction part is preferably 1 μL (microliter) or less, and particularly preferably 300 nL (nanoliter) or less. With such a small hollow reaction part 14, even if the diameter of the puncture device 21 is small, it is possible to collect a sufficient blood volume of the subject to be examined. Preferably, the diameter is 1000 μm or less.
The needle is preferably biodegradable.

  As described above, according to the biosensor chip 10 and the biosensor system 30 described above, the slider 20 having the puncture device 21 for puncture at the tip 20a is provided along the chip body 11 of the biosensor chip 10 and the sample collection port 12a is provided. Since it slides toward the provided tip 11a, the subject M can be easily punctured, and the puncture port and the sample collection port 12a are located at substantially the same position. A sample such as blood B flowing out from the puncture port can be reliably collected from the sample collection port 12a without positioning the mouth 12a at the puncture port. For this reason, analysis can be performed with a small amount of sample, and the burden on the subject can be reduced. In addition, since the puncture device 21 does not protrude from the chip body 11 before puncture or after puncture, it is possible to prevent a user or the like from being accidentally damaged during handling.

Next, a second embodiment of the biosensor chip according to the present invention will be described.
FIG. 5A is a plan view of the biosensor chip according to the second embodiment, and FIG. 5B is a front view of the biosensor chip according to the second embodiment. In addition, the same code | symbol is attached | subjected to the site | part which is common in the biosensor chip 10 concerning 1st Embodiment mentioned above, and the overlapping description is abbreviate | omitted.

In the first embodiment described above, the case where the puncture instrument 21 moves along the side surface 11d of the chip body 11 is illustrated, but as shown in FIG. 5, the biosensor chip 10B according to the second embodiment includes: The puncture device 21 moves along the upper surface 11b (or the lower surface) of the chip body 11. The slider 20B moves along the upper surface 11b of the chip body 11. The bending portion 24 that fixes the puncture device 21 is located at the center of the upper surface of the chip body 11, and the upper member 22 is provided on both the left and right sides. And the lower member 23 is connected via the left and right side plates 26. Further, the sample collection port 12 a is provided to be exposed at the center of the tip surface 11 a of the chip body 11, and the hollow reaction part 12 can be provided straight below the groove part 14.
Even if comprised in this way, the effect | action and effect similar to the biosensor chip 10 concerning 1st Embodiment mentioned above can be acquired.

The biosensor chip of the present invention is not limited to the above-described embodiments, and appropriate modifications and improvements can be made.
For example, the shape of the sliders 20 and 20B is not limited to the shape described above. Any shape that can move along the chip body 11 without dropping off may be used. Further, the shape of the biosensor chip 10 is not limited. Conventionally used rectangular biosensor chips can be used.

  Moreover, it is also possible to provide a groove part in which a part of the puncture device 21 is fitted and slid on the side surface 11d which is one surface of the chip body 11. For example, the groove portion preferably has a partial arc-shaped cross section having substantially the same size as the outer diameter of the puncture device 21. Thereby, the puncture device 21 can be fitted into the chip body 11 and smoothly slide along the chip body 11.

  As described above, the biosensor chip according to the present invention is easy because the slider having a puncture device at the tip slides toward the tip where the sample collection port is provided along the tip body of the biosensor chip. It is possible to puncture the subject at the same time, and after the puncture, the sample collection port can be reliably collected from the sample collection port without positioning the sample collection port at the puncture port. It is useful as a biosensor chip or the like for measuring and analyzing chemical substances using reagents contained in the hollow reaction part of the chip.

1 is a perspective view showing a first embodiment of a biosensor chip according to the present invention. (A) is a top view of a biosensor chip. (B) is a front view of a biosensor chip. The top view which shows embodiment of the biosensor system which concerns on this invention (A)-(C) are explanatory drawings which show the operation | movement which measures a blood glucose level using the biosensor system concerning this invention. (A) is a top view which shows 2nd Embodiment of the biosensor chip based on this invention. (B) is a front view showing a second embodiment of the biosensor chip according to the present invention. It is a disassembled perspective view which shows the conventional biosensor chip. (A) is a perspective view which shows the conventional biosensor chip. (B) is an exploded perspective view showing a conventional biosensor chip.

Explanation of symbols

10 Biosensor chip 11 Chip body 11a Tip 11d Side surface (one side)
12a Sample collection port 13a, 13b Detection electrode 14 Cover layer 20 Slider 20a Tip 21 Puncture device 25 Spacer layer 30 Biosensor system 31 Measuring instrument B Blood (sample)

Claims (4)

  A chip body having a sample collection port for collecting a sample at one end, and a slider provided with a puncture device at one end and slidable toward the tip of the chip body along the chip body Biosensor chip.   The biosensor chip according to claim 1, wherein the sampling port is located in the vicinity of a puncture position by the puncture device.   The biosensor chip according to claim 1, wherein the slider and the chip are fitted.   A biosensor system comprising: the biosensor chip according to any one of claims 1 to 3; and a measuring instrument that analyzes a sample collected by being connected to a detection electrode of the biosensor chip. .

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