JP2008167942A - Biosensor cartridge - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor cartridge capable of reducing a burden of a user by reducing a collection quantity of a sample necessary for a measurement and easily collecting and measuring the sample at a puncture hole while dispensing with a movement bringing a sample collection hole closer to the puncture hole. <P>SOLUTION: A puncture instrument 16 for the puncture is coveredly fixed to the outside face 12a of a substrate 12 with a resin 18 so as to be easily mounted on a chip body afterward. The distal end 16a of the puncture instrument 16 is projected near a sample collection hole 17 in the substrate 12, so that the sample is quickly collected from the sample collection hole 17 by puncturing it with the biosensor cartridge 10. This constitution can reduce the burden of the user by reducing the collection quantity of the sample necessary for the measurement and easily collect and measure the sample at the puncture hole while dispensing with the movement bringing the sample collection hole 17 closer to the puncture hole. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a biosensor cartridge for measuring and analyzing a chemical substance using a reagent accommodated in a hollow reaction part of a chip, for example.

Conventionally, for example, biosensors that detect the concentration of glucose in blood are known (see, for example, FIG. 1 of Patent Document 1).
FIG. 12 is an exploded perspective view showing the glucose sensor described as FIG. As shown in FIG. 12, a glucose sensor 100 that is a biosensor has a counter electrode 101 and a working electrode 102. The counter electrode 101 has a hollow puncture device shape cut in half in the length direction, and its distal end portion 103 is obliquely cut into an injection puncture device shape so as to facilitate puncturing. In addition, insulating layers 104 and 104 ′ that also serve as adhesive layers, such as epoxy resin adhesives, silicone adhesives, or glass, are generally applied to the cut surfaces that have been cut into half, and these insulating layers 104 and 104 ′ are applied. The working electrode 102 is attached via The working electrode 102 is a flat plate member on which glucose oxidase enzyme (GOD) is immobilized, and is adhered to the counter electrode 101 with the surface on which GOD is immobilized facing inward.
Therefore, blood is collected by puncturing the tip 103 of the puncturing instrument-like counter electrode 101 into the subject, and the reaction between the collected blood and the immobilized GOD 105 is detected by the working electrode 102 to determine glucose.

In addition, a biosensor in which a biosensor chip and a lancet are integrated is disclosed (see, for example, Patent Document 2).
13A is a perspective view of the sensor described as FIG. 1A of Patent Document 2, and FIG. 13B is an exploded view of the sensor described as FIG. 1B of Patent Document 2. It is a perspective view. As shown in FIG. 13, the lancet-integrated sensor 110 includes a chip body 111, a lancet 113, and a protective cover 115. The chip body 111 has a cover 111a and a substrate 111b that can be opened and closed, and an internal space 112 is formed on the inner surface of the cover 111a. The internal space 112 has a shape capable of movably storing the lancet 113, and the lancet 113 can be exchanged by opening the cover 111a.

  The puncture instrument 114 provided at the tip of the lancet 113 can be projected and retracted from an opening 112 a formed at the front end of the internal space 112 of the tip body 111 as the lancet 113 moves. The lancet 113 can be fixed to the chip body 111 by pressing both sides of the chip body 111 with a finger and pressing the projection 113a of the lancet 113, and puncture is performed in this fixed state. The protective cover 115 has a tube portion 115 a into which the puncture device 114 is inserted, and the tube portion 115 a can be accommodated inside the chip body 111 as the puncture device 114 moves. Therefore, in a state before use, the protective cover 115 is put on the puncture device 114 to protect the puncture device 114 and prevent accidental injury to the user. Note that the substrate 111b is provided with a pair of electrode terminals 116 so that the substrate 111b can be electrically connected to a measuring apparatus (not shown).

Therefore, in use, the protective cover 115 is removed, the lancet 113 is pushed, the puncture device 114 is protruded from the tip body 111, and both sides of the tip body 111 are pressed with fingers to fix the puncture device 114. After puncturing the subject in this state, the puncture instrument 114 is housed inside the chip body 111, and the opening 112a provided at the front end of the chip body 111 is brought close to the puncture port to collect the outflowed blood.
Japanese Patent Laid-Open No. 2-120655 (FIG. 1) International Publication No. 02/056769 Pamphlet (Figure 1)

However, in the glucose sensor 100 described in Patent Document 1, since the puncture instrument-like counter electrode 101 and the working electrode 102 are bonded together, the diameter of the puncture puncture instrument is approximately the same as the width of the glucose sensor 100 and is large. Become. For this reason, there is a problem in that the amount of blood collected increases, pain during puncture increases, and the burden on the user increases.
In addition, the lancet integrated sensor 110 described in Patent Document 2 does not have a structure that absorbs blood that has flowed out from the puncture opening from the opening 112a, so that a large amount of blood is flowed out in order to reliably collect blood. There is a problem that the burden on the user is increased.
In addition, the operation of bringing the measuring instrument close to the puncture opening after puncturing by the puncture device is troublesome, and there is also a problem that it is particularly burdensome for a user whose visual acuity has deteriorated due to age or diabetes.

  The purpose of the present invention is to reduce the burden on the user by reducing the amount of sample collected for measurement and to easily collect the sample at the puncture port without requiring the operation of bringing the sample collection port close to the puncture port. An object of the present invention is to provide a biosensor cartridge that can be measured.

  In order to achieve the above-described object, a biosensor cartridge according to the present invention includes two substrates facing each other, a spacer layer sandwiched between the two substrates, and at least one of the two substrates. A detection electrode provided on the surface of the substrate on the spacer layer side, a hollow reaction part formed by the two substrates and the spacer layer, and a sample collected by puncturing a specimen with a puncture device A biosensor cartridge having a sample collection port to be introduced into a hollow reaction part, wherein the puncture device is fixed to an outer surface of one of the two substrates, and the tip of the puncture device Projecting from the vicinity of the sampling port on the one substrate.

  In the biosensor cartridge configured as described above, the puncture device for puncture is fixed to the outer surface of the substrate, so that the puncture device can be easily attached to the chip body later, and the fixing material and the substrate Since the fixed area is increased, the mounting strength is increased as compared with the case where a bare puncture device is mounted using a tape or an adhesive. In addition, since the tip of the puncture device protrudes from the vicinity of the sample collection port in the substrate, it is possible to puncture with the biosensor cartridge and immediately collect the sample from the sample collection port and introduce it into the hollow reaction part. This reduces the burden on the user by reducing the amount of sample collected for measurement, and easily collects the sample from the puncture port without the need to move the sample collection port closer to the puncture port. Therefore, it is possible to reduce the burden on the user whose visual acuity has deteriorated. In the present invention, a needle, a lancet needle, a cannula and the like are collectively referred to as a puncture device.

  In addition, the biosensor cartridge according to the present invention has a puncture device holder on the outer surface of one of the two substrates, and the puncture device is provided at least in part of the puncture device holder. It is desirable that a slit corresponding to the shape is formed, and the puncture instrument is fixed in the slit.

  In the biosensor cartridge configured as described above, the puncture device is fitted into the slit provided in the puncture device holding portion, and the puncture device and the puncture device holding portion are integrated with a resin, for example, with a substrate. When it is fixed to the outer surface of the puncture device, it can be prevented from being retracted even if a pressing force is applied to the puncture device. Thereby, it can puncture reliably with the instrument for puncture.

  In the biosensor cartridge according to the present invention, it is preferable that a groove is formed in at least a part of one of the two substrates, and the puncture instrument is provided in the groove.

  In the biosensor cartridge configured as described above, since the puncture device is fitted and fixed in the groove provided on the substrate, the puncture device can be fixed near the sample collection port. For this reason, the distance between the puncture position by the puncture device and the sample collection port can be reduced, and even a small amount of sample can be reliably collected without performing an operation for adjusting the sample collection port to the puncture position.

  In the biosensor cartridge according to the present invention, it is preferable that a through hole is formed in at least a part of the two substrates, and the puncture device is provided in the through hole.

  In the biosensor cartridge configured as described above, the puncture device can be securely fixed at the through hole, so that the puncture device can be prevented from wobbling during puncture.

  In the biosensor cartridge according to the present invention, it is desirable that the tip of the puncture device is inclined toward the sample collection port provided at the tip of the hollow reaction part.

  In the biosensor cartridge configured as described above, since the tip of the puncture device is inclined toward the sample collection port, the distance between the puncture position by the puncture device and the sample collection port can be further reduced. Thus, even a small amount of sample can be reliably collected without performing an operation for adjusting the sample collection port to the puncture position.

  According to the present invention, the puncture device for puncture protrudes from the vicinity of the sampling port on the outer surface of the substrate and is fixed, so that the puncture device can be easily attached to the cartridge body later and the attachment strength is increased. can do. In addition, the sample can be collected from the sample collection port immediately after puncturing with the biosensor cartridge. For this reason, the amount of sample collected for measurement is reduced to reduce the burden on the user, and the sample at the puncture port is easily collected and measured without the need to move the sample collection port closer to the puncture port. The effect that it can do is acquired.

DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.
1A is an explanatory view of the first embodiment of the biosensor cartridge according to the present invention as viewed from the front, FIG. 1B is a side view of the biosensor cartridge as viewed from the direction B in FIG. 1A, and FIG. ) Is an end view seen from the direction C in FIG.

  As shown in FIGS. 1A to 1C, a biosensor cartridge 10A according to an embodiment of the present invention includes two substrates 11 and 12 facing each other, and the two substrates 11 and 12 sandwiched therebetween. A spacer layer 13 to be mounted, a detection electrode 14 provided on the surface of at least one of the two substrates 11, 12 on the spacer layer 13 side, the two substrates 11, 12, and the spacer layer 13. And a sample collection port 17 through which, for example, blood, which is a sample collected by puncturing a specimen with a puncture device 16, is introduced into the hollow reaction unit 15. Then, the puncture device 16 comes into contact with the outer surface 12a of one of the two substrates 11 and 12 and is covered and fixed by the resin 18, and the tip 16a of the puncture device 16 is fixed to the one substrate 11. Projecting from the vicinity of the sampling port 17 in FIG. Note that the fixing method is not limited to the above-described resin-fixed fixing method, and a fixing method other than the resin coating method may be employed.

  As shown in FIGS. 1A to 1C, the substrates 11 and 12 have an overall rectangular shape, and can be made of, for example, polyethylene terephthalate (PET). On the lower substrate 11 (left side in FIG. 1B), a pair of detection electrodes 14a and 14b are formed by printing on the substrate 11 with a material mainly composed of carbon, for example. ing. As shown by broken lines in FIG. 1A, the detection electrodes 14a and 14b are provided in a straight line in parallel with a predetermined interval, and the upper ends of both the detection electrodes 14a and 14b (FIG. 1A). In the middle upper end), only the upper substrate 12 and the substrate 11 below the spacer layer 13 are extended, and the upper surfaces of the detection electrodes 14a and 14b are exposed. Further, the lower end portions of the detection electrodes 14c and 14b are bent so as to face each other in an L shape, and are arranged at predetermined intervals in the hollow reaction portion 15.

  The spacer layer 13 can be formed using the same material as the substrates 11 and 12. The spacer layer 13 may be a single sheet, or may be formed by laminating a plurality of sheets. In this case, an adhesive that joins the plurality of spacer layers 13 also forms the spacer layer 13.

  The hollow reaction portion 15 is formed by the substrates 11 and 12 and the detection electrodes 14a and 14b on both upper and lower surfaces, and a rectangular space is formed with the spacer layer 13 cut into a predetermined shape as a side wall. For this reason, in the hollow reaction part 15, the detection electrodes 14a and 14b are exposed, and the reagent 19 is provided immediately above or in the vicinity of the detection electrodes 14a and 14b in the hollow reaction part 15. Therefore, the hollow reaction part 15 is a part where the sample B such as blood introduced from the sample collection port 17 undergoes a biochemical reaction with the reagent 19. For example, in the case of the biosensor chip 10A that measures the amount of glucose in blood, the reagent 19 is glucose oxidase, a glucose oxidase-electron acceptor (mediator) mixture layer, a glucose oxidase-albumin mixture layer, or a glucose oxidase-electron. A receptor-albumin mixture or the like is used. Enzymes other than glucose oxidase, such as glucose dehydrogenase, may be used, and a buffer, a hydrophilic polymer, or the like may be included in the reagent as an additive.

  A puncture device 16 is provided in contact with the outer surface 12a of the substrate 12, and the tip 16a of the puncture device 16 is fixed by a resin 18 so as to protrude from the tip of the biosensor cartridge 10A. The puncture device 16 is positioned in the vicinity of the sample collection port 17 (upward in FIG. 1C), which is the tip of the hollow reaction portion 15, in the cross section of the biosensor cartridge 10A, and is covered with the resin 18 and covered with the substrate. 12 is fixed. For this reason, it is desirable that the substrate 12 is formed as thin as possible and the puncture device 16 is as close as possible to the sample collection port 17. Thereby, after puncturing with the puncture device 16, blood flowing out from the puncture port can be collected without repositioning the biosensor cartridge 10A to the puncture port.

  When the biosensor cartridge 10A described above is manufactured, the puncture device 16 can be retrofitted to the biosensor cartridge 10A via the resin 18. In this case, the puncture device 16 is coated with the resin 18 in advance, and a flat surface 18a is formed on the coating on the side in contact with the outer surface 12a of the substrate 12, and the flat surface 18a is fixed to the substrate 12 with an adhesive or the like. For this reason, the adhesion area between the resin 18 and the substrate 12 can be increased, and the puncture device 16 can be firmly fixed. Note that the back of the puncture device 16 (upward in FIG. 1A) is firmly covered with a resin 18 so that it can resist the pressing force acting on the puncture device 16 during puncture, so that the puncture device 16 is capable of being punctured. Do not be pushed in and pushed in.

  According to the biosensor cartridge 10A described above, the puncture device 12 for puncture is covered and fixed to the outer surface 12a of the substrate 12 with the resin 18, so that the puncture device 16 can be easily attached to the biosensor cartridge 10A later. Since the bonding area is increased, the mounting strength is increased as compared with the case where the naked puncture device is attached with a tape or an adhesive. Further, it is possible to prevent the biosensor cartridge 10A from being crushed by pressurization or the reagent 19 from being altered by heating, as in the case where a naked puncture instrument is pressure-bonded by pressurization or heating. Further, since the tip 16a of the puncture device 16 protrudes from the vicinity of the sample collection port 17 in the substrate 12, puncture is performed by the biosensor cartridge 10A, and blood is immediately collected from the sample collection port 17 and introduced into the hollow reaction unit 15. can do. This reduces the burden on the user by reducing the amount of blood sampled for measurement, and easily collects blood after puncture without requiring the operation of bringing the sample collection port 17 close to the puncture port after puncture. Therefore, even a user whose visual acuity has deteriorated can be used easily.

Next, a biosensor cartridge according to a second embodiment of the present invention will be described.
FIG. 2 (A) is an explanatory view of a second embodiment of the biosensor cartridge according to the present invention as seen from the front, FIG. 2 (B) is a side view as seen from the B direction in FIG. 2 (A), and FIG. ) Is an end view seen from the direction C in FIG. 2A, FIG. 3A is a plan view of the film, and FIG. 3B is an end view of the film. In addition, the same code | symbol is attached | subjected to the site | part which is common in the site | part mentioned in the 1st Embodiment, and the overlapping description is abbreviate | omitted.

  As shown in FIGS. 2 (A) to (C), the biosensor cartridge 10B according to the present invention has a puncture device holder 21 on the outer surface 12a of one of the substrates 11 and 12, and A slit 22 corresponding to the shape of the puncture device 16 is formed at the distal end of the puncture device holding section 21, and the puncture device 16 is fixed in the slit 22. As shown in FIG. 3, a flat and rectangular puncture device holding part 21 is provided with a slit 22, and the whole is formed in a (reverse) U-shape. The width and length of the slit 22 are formed in accordance with the size and shape of the puncture device 16, and when the puncture device 16 is fitted into the slit 22, at least after the puncture device 16. The end face 16 b is brought into contact with the rear wall 22 a of the slit 22.

  Therefore, when the biosensor cartridge 10B described above is manufactured, the resin 18 does not adhere to the one surface 21a of 21 after the puncture device 16 is fitted into the slit 22 of the puncture device holding portion 21. The puncture device 16 and the puncture device holder 21 are integrated with the resin 18, and one surface 21 a of the puncture device holder 21 is fixed to the outer surface 12 a of the substrate 12 with an adhesive. Thereby, the puncture device 16 can be easily attached to the biosensor cartridge 10B.

  As described above, according to the biosensor cartridge 10B described above, it is possible to obtain the same operations and effects as those of the biosensor cartridge 10A according to the first embodiment described above. Furthermore, since the bonding surface between the puncture device holding part 21 for fixing the puncture device 16 and the substrate 12 can be increased, the attachment strength can be increased. In addition, since the puncture device 16 is fitted into the slit 22 provided in the puncture device holding section 21 to integrate the puncture apparatus 16 and the puncture device holding section 21, the force applied to the puncture apparatus 16 It is possible to prevent retraction even when acting, and to prevent the shaft center of the puncture device 16 from shaking. Thereby, it is possible to reliably puncture a desired puncture position with the puncture device 16. In addition, a plate-like film can be illustrated as an example of the material of the puncture device holding part, but is not limited thereto.

Next, a biosensor cartridge according to a third embodiment of the present invention will be described.
FIG. 4 (A) is an explanatory view of the third embodiment of the biosensor cartridge according to the present invention as seen from the front, FIG. 4 (B) is a side view as seen from the B direction in FIG. 4 (A), and FIG. ) Is an end view seen from the direction C in FIG. In addition, the same code | symbol is attached | subjected to the site | part which is common in the site | part mentioned above in 1st Embodiment or 2nd Embodiment, and the overlapping description is abbreviate | omitted.

  As shown in FIGS. 4A to 4C, in the biosensor cartridge 10C according to the present invention, a groove 12b is formed on the outer surface 12a of one of the substrates 11 and 12, and this groove The puncture device 16 is fixed to the resin 12b by 12b. The cross-sectional shape of the groove 12b can be exemplified by a V-shape, but may be U-shaped or rectangular. In addition, the length of the groove 12b may be a length that can accommodate the puncture device 16, but may be provided over the entire length of the substrate 12 from the viewpoint of workability. In the present embodiment, the V-shaped groove is formed, but other shapes are applicable, and may be, for example, a U-shape or a rectangular shape. Such a shape can be manufactured by processing with a laser or the like.

  Therefore, when manufacturing the above-described biosensor cartridge 10C, the groove 12b is formed in advance at least at the tip of the outer surface 12b of the substrate 12, and the puncture device 18 covered with the resin 18 is fitted into the groove 12b. The resin 18 is bonded to the substrate 12 in the combined state. It should be noted that it is desirable to form a flat surface on the bonding surface side of the resin 18 to ensure adhesion with the substrate 12.

  As described above, according to the biosensor cartridge 10C described above, it is possible to obtain the same operations and effects as those of the biosensor cartridge 10A according to the first embodiment described above. Further, since the groove 12b for fixing the puncture device 16 is provided, the distance between the puncture position by the puncture device 16 and the sample collection port 17 can be reduced, and the biosensor cartridge 10C performs the puncture. After that, blood can be immediately collected from the sample collection port 17 and introduced into the hollow reaction part 15. For this reason, the amount of blood collected for measurement is further reduced to reduce the burden on the user, and blood from the puncture port can be easily collected without requiring the operation of bringing the sample collection port 17 close to the puncture port. Therefore, it can be used easily even by a user with weak vision. In addition, since the puncture device 16 is fitted in the groove 12b, the puncture device 16 can be reliably punctured at a desired puncture position without being shaken during the puncture.

Next, a biosensor cartridge according to a fourth embodiment of the invention will be described.
5A is an explanatory view seen from the front showing a fourth embodiment of the biosensor cartridge according to the present invention, FIG. 5B is a side view seen from the direction B in FIG. 5A, and FIG. C) is an end view seen from the direction C in FIG. 5A, FIG. 6A is an explanatory view seen from the front of another example of the fourth embodiment of the biosensor cartridge according to the present invention, and FIG. FIG. 6B is a side view seen from the direction B in FIG. 6A, and FIG. 6C is an end view seen from the direction C in FIG. In addition, the same code | symbol is attached | subjected to the site | part which is common in the site | part mentioned above in 1st-3rd embodiment, and the overlapping description is abbreviate | omitted.

  As shown in FIGS. 5A to 5B and FIGS. 6A to 6C, in the biosensor cartridge 10D according to the present invention, the tip 16a of the puncture device 16 is provided at the tip of the hollow reaction portion 15. It is inclined toward the sample collection port 17. As a method of tilting and fixing the puncture device 16, the puncture device 16 can be tilted within the range of the thickness of the resin 18, as shown in FIGS. Alternatively, as shown in FIGS. 6A to 6C, an inclined groove 12c is provided on the outer surface 12a of the substrate 12, and the puncture device 16 is fitted into the groove 12c to be inclined. .

  Therefore, when the biosensor cartridge 10D described above is manufactured, the puncture device 16 is covered with the resin 18 in an inclined state, and the resin 18 is bonded to the outer side surface 12b of the substrate 12. At this time, if the inclined groove 12 c is provided in the substrate 12, the puncture device 16 is fitted into the groove 12 c and the resin 18 is bonded to the outer surface 12 a of the substrate 12. In addition, when providing the inclined groove | channel 12c, it is desirable to form the groove | channel 12c in the board | substrate 12 previously.

  As described above, according to the biosensor cartridge 10D described above, it is possible to obtain the same operations and effects as the biosensor cartridge 10A according to the first embodiment described above. Furthermore, since the tip of the puncture device 16 is tilted and fixed so as to approach the sample collection port 17 side, the distance between the puncture position by the puncture device 16 and the sample collection port 17 can be further reduced. Therefore, the blood sampling amount required for the measurement is further reduced to reduce the burden on the user, and blood from the puncture port can be easily collected without requiring the operation of bringing the sample sampling port 17 close to the puncture port. Can be measured. In particular, as shown in FIGS. 6A to 6C, when the inclined groove 12c is provided in the substrate 12, the distance between the puncture position and the sample collection port 17 can be further reduced. it can.

  In each of the embodiments described above, the substrate 12 on the side where the detection electrodes 14a and 14b are not provided is employed as the substrate 12 for fixing the puncture device 16, but as a modification, the detection electrodes 14a and 14b are provided. The puncture device 16 can be fixed to the provided substrate 11. In each of the above-described embodiments, the case where the puncture device 16 is previously coated with the resin 18 and the puncture device 16 is fixed by adhering the resin 18 to the substrate 12 has been described. It is also possible to position the puncture device 16 on the outer surface 12 a of the substrate 12 and fix the puncture device 16 with the resin 18. In this case, it is desirable to use a resin that is not pressurized or heated.

  In the embodiment described above, a groove is provided and a puncture device is provided in the groove. However, as another embodiment, a configuration in which a through hole is provided and the through hole is provided with a puncture device may be employed. it can. FIG. 7 shows an embodiment of a biosensor cartridge having a through hole. This biosensor cartridge 10G is provided with a spacer layer 13 and a detection electrode 14 between upper and lower substrates 11 and 12, a hollow reaction portion 15 is provided at the spacer layer 13, and a reagent 19 is accommodated therein. Yes. Two through holes 50 and 51 are formed so as to penetrate from the upper substrate 12 to the lower substrate 11. The leg portions 52 and 53 of the resin body 54 are inserted and fixed in these through holes 50 and 51. The resin body 54 has a substantially semicircular shape on the upper surface of the upper substrate 12. On the lower side of the semicircular resin body 54, that is, on the upper surface of the substrate 12, the puncture device 16 is embedded and fixed in the resin body 54, and the tip of the puncture device 16 is sampled. Projects forward from the mouth.

  FIG. 8 shows still another embodiment of the biosensor cartridge according to the present invention. In the biosensor cartridge 10E, a detection electrode 14E and a spacer layer 13E are disposed between two opposing substrates 11E and 12E. A hollow reaction portion 15E is formed at the spacer layer 13E, and a reagent 19E is accommodated in the hollow reaction portion 15E. Of the two substrates 11E and 12E, the puncture device 16E is fixed to the outer surface of the upper substrate, and these are entirely covered with the resin J. The biosensor cartridge 10E covered with the resin J has a circular cross-sectional shape and a substantially cylindrical shape. If it is the biosensor cartridge of such a shape, since it is covered with resin, the puncture device can be fixed securely. Furthermore, even when the biosensor cartridge is attached to the drive device of the puncture device and moved, the movement can be performed with good balance.

  FIG. 9 shows a modification of the biosensor cartridge shown in FIG. The biosensor cartridge 10F includes a detection electrode 14F, a spacer layer 13F, a hollow reaction portion 15F, and a reagent 19F between two substrates 11F and 12F, and a puncture device 16F is provided on the outer surface of the substrate 12F. These are covered with resin R. Protrusions 30 and 30 are formed on the distal end side of the biosensor cartridge 10F in a direction perpendicular to the longitudinal direction of the biosensor cartridge 10F, and the protrusions 30 and 30 are integrally formed with the resin R. The tip shapes of the protrusions 30 and 30 are corrugated with a depression 31 and 31 formed in the center. The depression 31 and 31 is easily gripped by a worker with a finger when the biosensor cartridge 10F is attached to the drive device. It is a shape that can be.

  FIG. 10 shows still another embodiment of the biosensor cartridge according to the present invention. In FIG. 10A, a puncture device 36 is fixed to a semicircular substrate 35, and this substrate 35 is integrally formed of resin by disposing the puncture device 36 in a mold (not shown). Created. As an example, detection electrodes 36 and 37 are formed on the upper surface of the semicircular substrate 35 by printing. Then, as shown in FIG. 10B, a spacer 38 is disposed on the electrodes 36 and 37 to form a hollow reaction part 41 containing a reagent 39, and a semicircular substrate 42 is further arranged. It is the biosensor cartridge 40 of the embodiment. The biosensor cartridge 40 has a substantially circular cross section, and can be stably moved when the biosensor cartridge 40 is attached to a driving device (not shown) and the cartridge is moved. Further, as shown in FIG. 10C, a biosensor cartridge 44 using a flat plate-like substrate 43 may be used as the upper substrate. In the biosensor chips 40 and 44 shown in FIGS. 10B and 10C, the puncture device 45 is disposed in the mold and integrally formed with resin, so that the puncture device can be securely fixed. it can. Furthermore, since the puncture device 45 is in the vicinity of the hollow reaction part 41, the biosensor cartridges 40, 44 are moved by the drive mechanism and puncture is performed by the puncture device 45, so that a test sample such as blood can be quickly and reliably hollow-reacted. Part 41 can be introduced.

  Furthermore, another embodiment of the biosensor cartridge according to the present invention is shown in FIG. This biosensor cartridge uses a biosensor chip similar to the biosensor chip shown in FIGS. 1 and 2 and a puncture device fixed on the substrate. That is, the detection electrode 14 and the spacer layer 13 are disposed between the upper and lower substrates 11 and 12, and the hollow reaction part 15 containing the reagent 19 is formed at the spacer layer 13. On the upper substrate 12 of the biosensor chip, there is a puncture device 51 covered with resin, and the bottom surface of the resin 50 and the upper surface of the substrate 12 are fixed with an adhesive as an example. The shape of the resin 50 is substantially semicircular or substantially semi-elliptical, and the width is substantially the same as the width of the substrate 12. Therefore, since the area which applies an adhesive agent can be taken large, the resin 50 and the board | substrate 12 can be fixed firmly. Therefore, a biosensor cartridge in which a puncture device is easily attached to an existing biosensor chip can be produced.

(A) is explanatory drawing seen from the front which shows 1st Embodiment of the biosensor cartridge which concerns on this invention. (B) is the side view seen from the B direction in FIG. 1 (A). (C) is the end view seen from the C direction in FIG. 1 (A). (A) is explanatory drawing seen from the front which shows 2nd Embodiment of the biosensor cartridge which concerns on this invention. (B) is the side view seen from the B direction in FIG. 2 (A). (C) is the end view seen from the C direction in FIG. 2 (A). (A) is a top view of a film. (B) is an end view of the film. (A) is explanatory drawing seen from the front which shows 3rd Embodiment of the biosensor cartridge which concerns on this invention. (B) is the side view seen from the B direction in FIG. 4 (A). FIG. 4C is an end view seen from the direction C in FIG. (A) is explanatory drawing seen from the front which shows 4th Embodiment of the biosensor cartridge which concerns on this invention. (B) is the side view seen from the B direction in FIG. 5 (A). FIG. 5C is an end view seen from the direction C in FIG. (A) is explanatory drawing seen from the front which shows another example of 4th Embodiment of the biosensor cartridge which concerns on this invention. (B) is the side view seen from the B direction in FIG. 6 (A). (C) is the end elevation seen from the C direction in FIG. 6 (A). It is a figure which shows another embodiment of the biosensor cartridge concerning this invention. It is a figure which shows another embodiment of the biosensor cartridge concerning this invention. It is a figure which shows the modification of the biosensor cartridge shown in FIG. It is a figure which shows another embodiment of the biosensor cartridge concerning this invention. It is a figure which shows another embodiment of the biosensor cartridge concerning this invention. It is a disassembled perspective view which shows the conventional biosensor cartridge. (A) is a perspective view which shows the conventional biosensor cartridge. (B) is an exploded perspective view showing a conventional biosensor cartridge.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Biosensor cartridge 11 Substrate 12 Substrate 12a Outer side surface 12b Groove 13 Spacer layer 14a, 14b Detection electrode 15 Hollow reaction part 16 Puncture device 16a Tip 17 Sampling port 18 Resin 21 Film 22 Slit

Claims (5)

Two substrates facing each other, a spacer layer sandwiched between the two substrates, a detection electrode provided on the surface of the at least one substrate of the two substrates on the spacer layer side, A biosensor cartridge having a hollow reaction portion formed by the two substrates and the spacer layer, and a sample collection port for introducing a sample collected by puncturing a specimen with a puncture device into the hollow reaction portion. ,
The puncture device is fixed to the outer surface of one of the two substrates, and the tip of the puncture device protrudes from the vicinity of the sampling port on the one substrate. A biosensor cartridge.   A puncture device holder is provided on the outer surface of one of the two substrates, and a slit corresponding to the shape of the puncture device is formed in at least a part of the puncture device holder. The biosensor cartridge according to claim 1, wherein the puncture device is fixed in a slit.   The biosensor cartridge according to claim 1, wherein a groove is formed in at least a part of one of the two substrates, and the puncture device is provided in the groove.   The biosensor cartridge according to claim 1, wherein a through hole is formed in at least a part of the two substrates, and the puncture device is provided in the through hole.   The biosensor cartridge according to any one of claims 1 to 3, wherein a tip of the puncture device is inclined toward a sample collection port provided at a tip of the hollow reaction part.

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