JP2009155333A - Anti-fatigue agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-fatigue agent using, as an active ingredient, a wasabi extract which is known to have functions such as a uric acid value-lowering action, an anti-stress action, a fat accumulation-inhibiting action, and a hyperglycemia-inhibiting action, and is furthermore effective for reducing fatigues. <P>SOLUTION: Provided is an anti-fatigue agent comprising the wasabi extract as an active ingredient. The fatigue is a phenomenon that a living body exhibits a function and consequently deteriorates the function. The anti-fatigue effect includes an action for reducing the fatigue state, an action for recovering the fatigue state, or an action for preventing the fatigue state. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an anti-fatigue agent comprising a horseradish extract as an active ingredient.

Wasabi extract has known functions such as a uric acid level lowering action (Patent Document 1), an anti-stress action (Patent Document 2), a fat accumulation inhibiting action (Patent Document 3), and a hyperglycemia disorder inhibiting action (Patent Document 4). It has been.
However, it is not known that the horseradish extract has the anti-fatigue action described below.

JP 2007-217366 A JP 2008-230975 A JP 2008-50373 A JP-A-2005-47822

  An object of the present invention is to provide an anti-fatigue agent having an excellent anti-fatigue action.

  The present invention provides an anti-fatigue agent comprising horseradish extract as an active ingredient.

ADVANTAGE OF THE INVENTION According to this invention, the anti-fatigue agent which has the outstanding anti-fatigue action can be provided. In addition, the fatigue in this invention means the phenomenon in which the function falls as a result of exhibiting a certain function. For example, “physical fatigue after swimming”, “mental fatigue after prolonged intellectual work”, “combined physical and mental fatigue that accumulates in everyday life”, etc. it can.
The anti-fatigue effect referred to in the present invention includes an action of reducing the fatigue state as described above, an action of recovering the fatigue state, an action of preventing the fatigue state, and the like.

  Hereinafter, the present invention will be described in more detail.

Wasabi used in the present invention is a plant belonging to the cross Plants, Sawa wasabi or field wasabi (Wasabiajaponica Matsum.), Horseradish (Armoraciarusticana Gaertn.), Yuriwasabi (Wasabiatenuis Matsum.), Cited Ezowasabi (Cardamineyezoensis Maxim.) It is done. Among them, the wasabi or field wasabi is preferable.

  Wasabi can utilize the whole plant or a part (for example, leaf, petiole, stem, rhizome, flower, etc.), but preferably stem and / or leaf.

A method for preparing the wasabi extract will be described.
As a pre-stage for preparing the horseradish extract, the portion of the horseradish used to obtain the extract (hereinafter referred to as sample) is preferably dried and pulverized.
The prepared sample is subjected to contact with an extraction solvent.
Examples of usable solvents include water, hot water; aqueous solvents containing lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, and propanol; lower alkyl esters such as ethyl acetate; ethylene glycol, propylene glycol, Examples include glycols such as glycerin; polar solvents such as acetone and acetic acid; hydrocarbons; known extraction solvents such as nonpolar solvents, preferably aqueous solvents, more preferably water or hot water (generally 50- 100 ° C.). Two or more of these solvents may be combined.
Note that the spicy component may be removed from the sample.

  The extraction can be carried out with or without stirring for a time during which sufficiently soluble components are extracted from the sample. The extraction time is appropriately determined depending on the type or temperature of the extraction solvent, but is typically 30 minutes to 48 hours.

  Specifically, in a preferred form, a freeze-dried sample is pulverized, extracted with 10 to 20 volumes of water or hot water, and centrifuged to obtain a wasabi extract. Moreover, as described in Patent Document 1, the supernatant obtained by centrifugation may be brought into contact with several volumes of ethanol to perform an ethanol precipitation operation. After this operation, filtration is performed, and the filtrate is concentrated under reduced pressure to obtain a wasabi extract.

In a more preferred form of the invention, the horseradish extract is a water-soluble, non-volatile component that does not precipitate by ethanol precipitation.
Examples of the ethanol precipitate include nucleic acids, polysaccharides, proteins, and the like, but these components may or may not be included in the anti-fatigue agent of the present invention.

  The dose of the horseradish extract varies depending on the age, body weight, indication symptoms, etc. of the patient. For example, as a lyophilized powder, about 1 mg to 50 g, preferably 1 g to 10 g, per day for an adult. It is better to administer to some extent.

  The anti-fatigue agent of the present invention can be appropriately prepared by a known method in the form of a solid or solution (hereinafter also referred to as a preparation) such as tablets, pills, capsules, granules, powders, powders, and liquids. That is, the solid preparation or liquid preparation useful in the present invention is produced by using a well-known preparation method that has been well established. Examples of additives include excipients, pH adjusting agents, cooling agents, suspending agents, diluents, antifoaming agents, thickeners, solubilizers, disintegrating agents, binders, lubricants, antioxidants. Agents, coating agents, coloring agents, flavoring agents, surfactants, plasticizers or fragrances.

  The anti-fatigue agent of the present invention can be taken as various health foods and functional foods. Examples of these include various products, but for the production of health foods and functional foods, in addition to commonly used food materials and food additives, excipients, extenders, binders, It can be used in the form of food and drink preparations using adjuvants such as disintegrants, lubricants, dispersants, preservatives, wetting agents, solubilizers, preservatives, stabilizers, capsule bases and the like. Specific examples of the adjuvant include lactose, fructose, glucose, starch, gelatin, magnesium carbonate, synthetic magnesium silicate, talc, magnesium stearate, calcium carbonate, methylcellulose, carboxymethylcellulose, or a salt thereof, gum arabic , Polyethylene glycol, syrup, petrolatum, glycerin, ethanol, propylene glycol, citric acid, sodium chloride, sodium sulfite, sodium phosphate, pullulan, carrageenan, dextrin, reduced palatinose, sorbitol, xylitol, stevia, synthetic sweetener, citric acid Ascorbic acid, acidulant, baking soda, sucrose ester, hardened vegetable oil, potassium chloride, safflower oil, beeswax, soybean lecithin, flavor, and the like can be blended. Regarding the production of such health foods and functional foods, reference books for pharmaceutical preparations such as “Japanese Pharmacopoeia Manual (General Rules for Preparations)” (Yodogawa Shoten) can be referred to.

  In addition to the above, the anti-fatigue agent of the present invention can be taken as a food or drink. Specifically, natto, thick fried, tofu, konjac, dumplings, pickles, boiled, croquettes, sandwiches, pizzas, hamburgers, dumplings, shumai, salads, etc., various powders (beef, pork, chicken and other livestock products) Marine products such as shrimp, scallops, sea bream, kelp, vegetables / fruits, plants, yeast, algae, etc.), pudding, cookies, crackers, bread, cakes, chocolate, potato chips, biscuits, donuts, jellies and other confectionery, Japanese rice crackers such as rice crackers, sheep crab, daifuku, ohagi, other buns, Japanese confectionery such as castella, frozen confectionery (rice cake etc.), bread and confectionery such as chewing gum, noodles such as udon, buckwheat and kishimen, kamaboko, ham, fish sausage, etc. Fish paste products, meat products such as ham, sausage, hamburger, corn beef, salt, pepper, miso, soy sauce, sauce, dressy Seasonings such as gyu, mayonnaise, ketchup, sweeteners, spices, teppanyaki foods such as Akashi-yaki, takoyaki, monja-yaki, okonomiyaki, yakisoba, fried udon, dairy products such as cheese, hard-type yogurt, and fats and oils Various foods such as powdered solids of flavors and fragrances (vanilla, citrus fruits, bonito etc.) and powdered foods and drinks (instant coffee, instant tea, instant milk, instant soup, miso soup, etc.) These are not particularly limited.

  Furthermore, in the present invention, for example, royal jelly, propolis, vitamins (A, C, D, E, K, folic acid, pantothenic acid, biotin, derivatives thereof, etc.), minerals (iron, magnesium, calcium, zinc, etc.), Selenium, lecithin, carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), saponins (gymnemic acid, soybean saponin, ginseng saponins, etc.), fatty acids, proteins (collagen, elastin, etc.), oligosaccharides (isomalto-oligosaccharides, cyclic oligosaccharides, etc.) ), Phospholipids and derivatives thereof (phosphatidylcholine, sphingomyelin, ceramide, etc.), sulfur-containing compounds (eg, alliin, sepaene, taurine, glutathione, methylsulfonylmethane), sugar alcohols, lignans (sesamin, etc.), Contains animal and plant extracts Root vegetables (turmeric, ginger, etc.), may be used in combination and the like.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further, this invention is not limited to these.

Example 1
Wasabi extract was prepared according to the method described in Patent Document 1.
Saw wasabi stems (produced in Izu City, Shizuoka Prefecture) were freeze-dried and ground. To 100 g of the pulverized product, 1.5 L of water was added, followed by extraction by shaking for 30 minutes, followed by centrifugation, and 2.3 times the volume of ethanol was added to and mixed with the supernatant. For more than 5 minutes. The precipitate was removed by filtration and then concentrated under reduced pressure to obtain a wasabi extract having a solid content of 18%. Hereinafter, this is called extract 1.
Separately, 1.5 L boiling water is added to 100 g of the pulverized product, and after 30 minutes of stirring and extraction, centrifugation is performed, and 2.3 times the volume of ethanol is added to the supernatant.・ Mixed and left at room temperature for more than 5 minutes. The precipitate was removed by filtration and then concentrated under reduced pressure to obtain a wasabi extract having a solid content of 18%. Hereinafter, this is called extract 2.

  STD DDY male mice (5 weeks old: each group n = 3 to 4) were orally ingested with the above extract 1. The intake is 100 mg / kg body weight. Ingestion was performed using a solution in which the extract 1 was dissolved in pure water. The control group mice were tested by ingesting pure water only.

Thirty minutes after the ingestion, the mouse was placed in an 80 cm deep water tank, and the time until immobility was measured. As an average value of time until the mice of each test group (n = 3 to 4) reached immobility, the control group was about 100 seconds, while the extract 1 administration group was about 390 seconds. Met.
From the above, it was confirmed that Extract 1 has a high anti-fatigue effect.

Example 2
Twelve volunteer men (age 34-37) were grouped into a test meal group (Example) and a placebo group (Comparative Example). The test meal group (Example) is a group in which Extract 1 is processed into a soft capsule based on olive oil and 1 g of Extract 1 is ingested daily. The placebo group (comparative example) is a group in which soft capsules containing olive oil not containing extract 1 are ingested daily.
Volunteer men were exercised with a bicycle ergometer at 5 and 9 weeks after ingestion. The exercise continues for 30 minutes at an exercise intensity of 80% load of the maximum heart rate.
The blood lactate levels of volunteer men immediately before and after exercise were measured with a commercially available simple blood lactate analyzer, and the amount of blood lactate increased was examined.
As a result, the increase after 5 weeks from the start of ingestion was about 6.25 mmol / l in the comparative example compared to about 6 mmol / l in the comparative example.
The increase after 9 weeks from the start of ingestion was about 6 mmol / l in the comparative example, whereas it was about 4.7 mmol / l in the example.
From the above, the anti-fatigue action of Extract 1 was clarified.

In each of the above examples, the same results as above were obtained when leaves were used instead of wasabi stems and when extract 2 was used instead of extract 1. Further, when the extract 1 of Example 1 was prepared, the same results as above were obtained even when the stem of horseradish ( Armorciaciaticicana Gaertn.) Was used as a raw material.

The compounding example of the anti-fatigue agent of this invention is shown below.
Candy sugar 50.0wt%
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Anti-fatigue agent of the present invention Total remaining amount 100.0 wt%

Yogurt milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Anti-fatigue agent of the present invention 1.0
Perfume Trace water Residue
Total 100.0wt%

Soft drink fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Anti-fatigue agent of the present invention 1.0
Perfume Appropriate amount of water Residual
Total 100.0wt%

Claims (1)

  Anti-fatigue agent containing horseradish extract as an active ingredient.