JP2009136250A5 - Biological sample reaction apparatus and biological sample reaction method - Google Patents
Biological sample reaction apparatus and biological sample reaction method Download PDFInfo
- Publication number
- JP2009136250A5 JP2009136250A5 JP2007318627A JP2007318627A JP2009136250A5 JP 2009136250 A5 JP2009136250 A5 JP 2009136250A5 JP 2007318627 A JP2007318627 A JP 2007318627A JP 2007318627 A JP2007318627 A JP 2007318627A JP 2009136250 A5 JP2009136250 A5 JP 2009136250A5
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- reaction
- sample reaction
- chip
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012472 biological sample Substances 0.000 title claims description 35
- 239000012295 chemical reaction liquid Substances 0.000 claims 6
- 230000005484 gravity Effects 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 239000011810 insulating material Substances 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 230000002093 peripheral Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 1
Description
以下、本発明の実施の形態について図面を参照して説明する。
実施の形態１．
図１は、本発明の実施の形態１によるＰＣＲ用チップ（生体試料反応用チップ）１０と、ＰＣＲ反応装置（生体試料反応装置）２０の構成を示す斜視図である。図に示すように、ＰＣＲ用チップ１０は、透明基板１０１，１０２を貼り合わせて構成されており、第１のウェル（第１の反応部）１０３、第２のウェル（第２の反応部）１０４、第３のウェル（第３の反応部）１０５、第１の流路１０６、第２の流路１０７が形成されている。透明基板１０１，１０２は例えばガラス基板とすることができる。各々１つの第１のウェル１０３、第２のウェル１０４、第３のウェル１０５、第１の流路１０６、及び第２の流路１０７によって１つの反応系が形成される。本実施形態では、ＰＣＲ用チップ１０は１つの基板上に７個の反応系を有している。
Hereinafter, embodiments of the present invention will be described with reference to the drawings.
Embodiment 1 FIG.
FIG. 1 is a perspective view showing the configuration of a PCR chip (biological sample reaction chip) 10 and a PCR reaction apparatus (biological sample reaction apparatus) 20 according to Embodiment 1 of the present invention. As shown in the figure, the PCR chip 10 is formed by bonding transparent substrates 101 and 102, and includes a first well (first reaction unit) 103 and a second well (second reaction unit). 104, a third well (third reaction portion) 105, a first flow path 106, and a second flow path 107 are formed. The transparent substrates 101 and 102 can be glass substrates, for example. One reaction system is formed by each of the first well 103, the second well 104, the third well 105, the first flow path 106, and the second flow path 107. In this embodiment, the PCR chip 10 has seven reaction systems on one substrate.
Claims (9)
水平方向の回転軸と、
前記収容部に前記生体試料反応用チップを収容した場合に、前記回転軸から外側へ向かうにつれて温度が低くなる温度勾配が形成されるように前記生体試料反応用チップを加熱する温度調節手段と、を含み、
前記収容部に前記生体試料反応用チップを収容した場合に、前記第１の反応部と前記回転軸との距離が、前記第２の反応部と前記回転軸との距離よりも小さい、
生体試料反応装置。 A first reaction part, a second reaction part, a first flow path connecting the first reaction part and the second reaction part, and the reaction liquid and the reaction liquid are mixed with each other And a storage unit for storing a biological sample reaction chip filled with a liquid having a specific gravity smaller than that of the reaction liquid,
A horizontal axis of rotation;
A temperature adjusting means for heating the biological sample reaction chip so as to form a temperature gradient in which the temperature decreases toward the outside from the rotating shaft when the biological sample reaction chip is stored in the storage unit; Including
When the biological sample reaction chip is accommodated in the accommodating portion, a distance between the first reaction portion and the rotation shaft is smaller than a distance between the second reaction portion and the rotation shaft.
Biological sample reaction device.
前記温度調節手段は、
前記回転軸に設けられた第１のヒータと、
前記生体試料反応装置の外周面近傍に設けられ、前記第１のヒータよりも温度が低い第２のヒータと、
を含む、
生体試料反応装置。 The biological sample reaction apparatus according to claim 1,
The temperature adjusting means includes
A first heater provided on the rotating shaft;
A second heater provided in the vicinity of the outer peripheral surface of the biological sample reaction device and having a temperature lower than that of the first heater;
including,
Biological sample reaction device.
前記第１のヒータと前記第２のヒータとの間に設けられた断熱材をさらに含む、
生体試料反応装置。 The biological sample reaction apparatus according to claim 2,
A heat insulating material provided between the first heater and the second heater;
Biological sample reaction device.
前記収容部を複数含む、
生体試料反応装置。 The biological sample reaction device according to any one of claims 1 to 3,
Including a plurality of the accommodating portions,
Biological sample reaction device.
複数の前記収容部は、前記回転軸に対して放射状に配置されている、
生体試料反応装置。 The biological sample reaction apparatus according to claim 4,
The plurality of accommodating portions are arranged radially with respect to the rotation axis.
Biological sample reaction device.
前記生体試料反応用チップを収容した場合に前記生体試料反応用チップの内部を測定する測定用ポートをさらに含む、
生体試料反応装置。 The biological sample reaction apparatus according to any one of claims 1 to 5,
A measurement port for measuring the inside of the biological sample reaction chip when the biological sample reaction chip is accommodated;
Biological sample reaction device.
前記生体試料反応用チップは、
第３の反応部と、前記第２の反応部と前記第３の反応部とを接続する第２の流路と、をさらに含み、
前記収容部に前記生体試料反応用チップを収容した場合に、前記第２の反応部と前記回転軸との距離が、前記第３の反応部と前記回転軸との距離よりも小さい、
生体試料反応装置。 The biological sample reaction apparatus according to claim 1,
The biological sample reaction chip is:
A third reaction part, and a second flow path connecting the second reaction part and the third reaction part,
When the biological sample reaction chip is accommodated in the accommodating portion, the distance between the second reaction portion and the rotation shaft is smaller than the distance between the third reaction portion and the rotation shaft.
Biological sample reaction device.
前記収容部はスリットである、
生体試料反応装置。 The biological sample reaction device according to any one of claims 1 to 7,
The accommodating portion is a slit;
Biological sample reaction device.
第１の反応部と、第２の反応部と、前記第１の反応部と前記第２の反応部とを接続する第１の流路とを含み、反応液と、前記反応液とは混和せず、かつ、前記反応液よりも比重の小さい液体とが充填された生体試料反応用チップを収容部に収容することと、
前記収容部に前記生体試料反応用チップを収容した場合に、回転軸から外側へ向かうにつれて温度が低くなる温度勾配が形成されるように前記生体試料反応用チップを加熱することと、
前記生体試料反応装置を水平方向の前記回転軸の周りに回転させることと、
を含む、
生体試料反応方法。 A biological sample reaction method using a biological sample reaction device,
A first reaction part, a second reaction part, a first flow path connecting the first reaction part and the second reaction part, and the reaction liquid and the reaction liquid are mixed with each other And storing a biological sample reaction chip filled with a liquid having a specific gravity smaller than that of the reaction liquid in the storage part;
Heating the biological sample reaction chip so as to form a temperature gradient in which the temperature decreases toward the outside from the rotation axis when the biological sample reaction chip is stored in the storage unit;
Rotating the biological sample reactor around the horizontal axis of rotation;
including,
Biological sample reaction method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007318627A JP5196126B2 (en) | 2007-12-10 | 2007-12-10 | Biological sample reaction apparatus and biological sample reaction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007318627A JP5196126B2 (en) | 2007-12-10 | 2007-12-10 | Biological sample reaction apparatus and biological sample reaction method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009136250A JP2009136250A (en) | 2009-06-25 |
| JP2009136250A5 true JP2009136250A5 (en) | 2011-01-13 |
| JP5196126B2 JP5196126B2 (en) | 2013-05-15 |
Family
ID=40867574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007318627A Active JP5196126B2 (en) | 2007-12-10 | 2007-12-10 | Biological sample reaction apparatus and biological sample reaction method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5196126B2 (en) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630056B (en) * | 2010-01-12 | 2017-11-07 | 阿赫姆生物系统公司 | Two benches thermal convection current device and application thereof |
| JP2011147411A (en) * | 2010-01-25 | 2011-08-04 | Seiko Epson Corp | Method and apparatus for amplifying nucleic acid, and chip for amplifying nucleic acid |
| JP2011155921A (en) * | 2010-02-02 | 2011-08-18 | Seiko Epson Corp | Biochip, specimen reactor, and method for specimen reaction |
| JP5764870B2 (en) | 2010-04-14 | 2015-08-19 | セイコーエプソン株式会社 | Biochip, reaction apparatus and reaction method |
| JP5862006B2 (en) * | 2010-11-17 | 2016-02-16 | セイコーエプソン株式会社 | Thermal cycling apparatus and thermal cycling method |
| JP5867668B2 (en) * | 2010-12-01 | 2016-02-24 | セイコーエプソン株式会社 | Thermal cycling apparatus and thermal cycling method |
| JP5773119B2 (en) * | 2010-12-14 | 2015-09-02 | セイコーエプソン株式会社 | Biochip |
| JP5899624B2 (en) * | 2011-02-18 | 2016-04-06 | セイコーエプソン株式会社 | Reaction vessel |
| JP5896100B2 (en) * | 2011-03-01 | 2016-03-30 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP2012239441A (en) | 2011-05-23 | 2012-12-10 | Seiko Epson Corp | Reaction vessel |
| JP5849443B2 (en) * | 2011-06-07 | 2016-01-27 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP5842391B2 (en) * | 2011-06-07 | 2016-01-13 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP5853494B2 (en) * | 2011-08-25 | 2016-02-09 | セイコーエプソン株式会社 | Thermal cycle apparatus and abnormality determination method |
| JP5896110B2 (en) * | 2011-09-21 | 2016-03-30 | セイコーエプソン株式会社 | Thermal cycle device and control method for thermal cycle device |
| JP5935981B2 (en) * | 2012-03-28 | 2016-06-15 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP5935980B2 (en) * | 2012-03-28 | 2016-06-15 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP5971463B2 (en) | 2012-03-30 | 2016-08-17 | セイコーエプソン株式会社 | Thermal cycle device and control method for thermal cycle device |
| JP6216494B2 (en) * | 2012-03-30 | 2017-10-18 | セイコーエプソン株式会社 | Thermal cycle device and control method for thermal cycle device |
| JP2013208066A (en) | 2012-03-30 | 2013-10-10 | Seiko Epson Corp | Thermal cycler and control method of thermal cycler |
| JP5935985B2 (en) * | 2012-03-30 | 2016-06-15 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP5910304B2 (en) * | 2012-05-22 | 2016-04-27 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP6090554B2 (en) * | 2012-05-30 | 2017-03-08 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP6011036B2 (en) | 2012-06-06 | 2016-10-19 | セイコーエプソン株式会社 | device |
| JP2013252091A (en) * | 2012-06-06 | 2013-12-19 | Seiko Epson Corp | Thermal cycler |
| JP5967361B2 (en) | 2012-06-06 | 2016-08-10 | セイコーエプソン株式会社 | Heat cycle equipment |
| MX350425B (en) * | 2012-06-28 | 2017-09-05 | Fluoresentric Inc | A chemical indicator device. |
| JP5939392B2 (en) * | 2012-07-18 | 2016-06-22 | セイコーエプソン株式会社 | Container and thermal cycle equipment |
| WO2014035124A1 (en) * | 2012-08-30 | 2014-03-06 | (주) 메디센서 | Rotary pcr device and pcr chip |
| JP2014135941A (en) * | 2013-01-18 | 2014-07-28 | Seiko Epson Corp | Thermal cycler and thermal cycling method |
| JP2014176302A (en) | 2013-03-13 | 2014-09-25 | Seiko Epson Corp | Nucleic acid amplification reaction device and nucleic acid amplification method |
| JP2015023844A (en) | 2013-07-29 | 2015-02-05 | セイコーエプソン株式会社 | Nucleic acid amplification reaction apparatus, and nucleic acid amplification method |
| JP2015073485A (en) | 2013-10-09 | 2015-04-20 | セイコーエプソン株式会社 | Nucleic acid amplification method, device for nucleic acid extraction, cartridge for nucleic acid amplification reaction, and kit for nucleic acid amplification reaction |
| JP2015104364A (en) | 2013-11-29 | 2015-06-08 | セイコーエプソン株式会社 | Container for nucleic acid amplification reaction, cartridge for nucleic acid amplification reaction, and cartridge kit for nucleic acid amplification reaction |
| JP2015154722A (en) | 2014-02-20 | 2015-08-27 | セイコーエプソン株式会社 | Nucleic acid amplification reaction vessel and nucleic acid amplification reaction device |
| JP2015181458A (en) | 2014-03-26 | 2015-10-22 | セイコーエプソン株式会社 | Nucleic acid amplification reaction device and nucleic acid amplification method |
| JP2015181457A (en) | 2014-03-26 | 2015-10-22 | セイコーエプソン株式会社 | Nucleic acid amplification reaction device and nucleic acid amplification method |
| JP2015188343A (en) * | 2014-03-27 | 2015-11-02 | セイコーエプソン株式会社 | Biochip |
| JP2015188378A (en) * | 2014-03-28 | 2015-11-02 | セイコーエプソン株式会社 | Nucleic acid analysis apparatus and nucleic acid analysis method |
| JP2015188377A (en) | 2014-03-28 | 2015-11-02 | セイコーエプソン株式会社 | Cartridge for nucleic acid amplification reaction and nucleic acid amplification device |
| JP2015223083A (en) * | 2014-05-26 | 2015-12-14 | セイコーエプソン株式会社 | Control method of nucleic acid amplification reaction device |
| JP6020860B2 (en) * | 2015-07-02 | 2016-11-02 | セイコーエプソン株式会社 | Biochip |
| JP6120030B2 (en) * | 2016-01-08 | 2017-04-26 | セイコーエプソン株式会社 | Heat cycle equipment |
| JP6206688B2 (en) * | 2016-05-11 | 2017-10-04 | セイコーエプソン株式会社 | Heat cycle equipment |
| CN108796038B (en) * | 2018-06-26 | 2019-10-18 | 杭州优思达生物技术有限公司 | A kind of nucleic acid integrated detection method and detection reagent pipe |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4095886B2 (en) * | 2002-05-08 | 2008-06-04 | 株式会社日立ハイテクノロジーズ | Chemical analysis device and genetic diagnosis device |
| EP1628754A2 (en) * | 2003-06-05 | 2006-03-01 | Bioprocessors Corporation | Apparatus and method for manipulating substrates |
| EP1754785A1 (en) * | 2004-05-27 | 2007-02-21 | Universal Bio Research Co., Ltd. | Reaction vessel, reaction measuring device, and liquid rotating treatment device |
| CA2611955A1 (en) * | 2005-06-16 | 2006-12-28 | Stratagene California | Heat blocks and heating |
| KR20080005269A (en) * | 2006-03-24 | 2008-01-10 | 가부시끼가이샤 도시바 | Nucleic acid detection cassette and nucleic acid detection apparatus |
| JP3980045B2 (en) * | 2006-05-01 | 2007-09-19 | 技術研究組合医療福祉機器研究所 | DNA amplification equipment |
-
2007
- 2007-12-10 JP JP2007318627A patent/JP5196126B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2009136250A5 (en) | Biological sample reaction apparatus and biological sample reaction method | |
| TWI797120B (en) | Fluidic test cassette | |
| JP7167102B2 (en) | Fluid inspection cassette | |
| US9895690B2 (en) | Microfluidic chip and application thereof | |
| US10023897B2 (en) | Biosensor chip, and biosensor device equipped with same | |
| JP2011523698A5 (en) | ||
| US20110183378A1 (en) | Nucleic acid amplification method, nucleic acid amplification apparatus, and chip used in nucleic acid amplification | |
| TW200938841A (en) | Reaction chip and reaction method, temperature adjustment mechanism for gene processing device and gene processing device | |
| ES2913464T3 (en) | biochemical reaction system | |
| CN110191759A (en) | Micro-fluidic sample chip uses the analysis system of the chip and the PCR method for detecting DNA sequence dna | |
| JP2011227083A5 (en) | ||
| JP6800510B2 (en) | Bioreactor system with temperature sensor | |
| KR101513273B1 (en) | A rotary type PCR machine and a PCR chip | |
| CN102237261A (en) | Method and device for loosening polymer coating from substrate surface | |
| JP2012501950A5 (en) | ||
| US20070243109A1 (en) | Micro-scale heating module | |
| TW201910551A (en) | Chemical vapor deposition system | |
| CN215220674U (en) | Wafer boat and diffusion equipment | |
| CN112912721A (en) | High sample flux differential scanning calorimeter | |
| JP5407445B2 (en) | Temperature control device | |
| Morganti et al. | A micro polymerase chain reaction module for integrated and portable DNA analysis systems | |
| JP4454317B2 (en) | Substrate processing equipment | |
| JP2011217659A (en) | Biochip and temperature controller | |
| JP5919710B2 (en) | Heat cycle equipment | |
| JP6081071B2 (en) | Nucleic acid detection device |