JP2009120517A - Intestinal flora improver - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To find a new component that has an intestinal flora improving action and is safe. <P>SOLUTION: The intestinal flora improver comprises a xyloglucuronomannan composed of α-1,3-mannose as a main chain and D-xylose and D-glucuronic acid as side chains in which the constitution ratio [α-1,3-mannose: D-glucuronic acid] of the α-1,3-mannose in the xyloglucuronomannan to the D-glucuronic acid of 9:1-9:3.5 (molar). The xyloglucuronomannan is preferably extracted from species of heterobasidiomycetes classified as the genus Tremella. The intestinal flora improver is useful as a medicine, a food and beverage, a cosmetic etc. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an intestinal flora improving agent comprising a novel ingredient as an active ingredient. The present invention is widely used for pharmaceuticals, quasi drugs, foods, external preparations for skin, and the like.

  Various bacteria are resident in the intestinal tract of humans and animals, forming a complex intestinal flora. The bacterial species constituting the intestinal flora are classified into three bacterial groups according to the pattern of transition with age and the relationship established with the host (see Non-Patent Document 1 and Non-Patent Document 2).

Group I appears a little later after birth, but is the most prevalent group of normal intestinal flora. This group is highly animal specific and maintains a symbiotic relationship with the host throughout its lifetime without being eliminated or suppressed by the host defense mechanism. This group I includes Bifidobacterium (hereinafter abbreviated as Bifidobacterium), Eubacterium (hereinafter abbreviated as Eubacterium), Bacteroidaceae (hereinafter referred to as Bacteroidaceae: Bacteroides (abbreviated as Bacteroides), Peptococcus (hereinafter abbreviated as Peptococcus), Lactobacillus (hereinafter abbreviated as Lactobacillus), Escherichia (abbreviated as Escherichia) A group is included.
Among these, Bifidobacterium and Lactobacillus are useful enteric bacteria that have been scientifically proven useful for animals and humans.

  Group II appears shortly after birth and once becomes the most dominant fungal group, but it is replaced by the soon-to-be-exploited group I fungus group, and then remains at a moderate number of bacteria. Enterobacteriaceae (hereinafter abbreviated as Enterobacteriaceus) and Streptococcus (hereinafter abbreviated as Streptococcus), which tend to increase when it gets worse, are included. Group III belongs to the minority group in the intestinal flora and belongs to the category of pathogenic bacteria. It breaks the defense mechanism of the host and invades a place that did not exist so far, so-called spontaneous infection (also called opportunistic infection). ) Is a group of bacteria that may exert pathogenicity. This group III includes a group of bacteria belonging to the genus Clostridium (hereinafter abbreviated as Clostridium), pathogenic enterobacteria, Staphylococcus (hereinafter abbreviated as Staphylococcus). It is.

  Intestinal flora is fairly stable in healthy individuals, but varies with host physiology, disease, drugs, food, stress, aging, intestinal fungal interactions, and more. The pattern of abnormal flora is nonspecific, and the total number of bacteria increases significantly in the small intestine, especially various anaerobic bacteria, enterobacteria, or streptococcus. In the large intestine, a decrease or disappearance of bifidobacteria, a marked increase in aerobic bacteria, especially enterobacterial, streptococcus, and sometimes an increase in Clostridium perfringens are considered to be common fluctuations in abnormal flora (non-patent literature). 3). In this way, fungal groups belonging to Group II and Group III are involved in the formation of abnormal intestinal flora by intestinal growth, and may be pathogenic depending on the health condition of the host, and thus are considered enteric harmful bacteria. .

  Advances in research related to intestinal flora seem to be considered critical to maintaining good intestinal flora or improving deteriorated intestinal flora to maintain the health of the host Became. From such a background, as a food ingredient for maintaining and improving intestinal flora, so-called probiotics that are viable bacteria additives such as Bifidobacterium and Lactobacillus, or intestines such as Bifidobacterium So-called prebiotics such as lactulose and fructooligosaccharide, which have a function of increasing useful bacteria in the intestine, have been put into practical use.

  In order to improve intestinal flora, maintain or increase useful intestinal bacteria such as Bifidobacterium, suppress enterobacteria such as enterobacteriaceae, streptococcus, staphylococcus, clostridium, It is important that eschericia, one of the useful intestinal bacteria, tends to approach a predetermined reference value.

Classification and ecology of the intestinal flora, Tomoashi Mitsuoka, published by the Dietary Life Research Society, pages 51-52, 1986 Intestinal flora structure and function, Yoshiyuki Morishita, Asakura Shoten, 8-10, 1990 "Intestinal flora and health", Tomoashi Mitsuoka, published by Kansai Center, pages 157-158, 1998

  Under such a background, as a result of human tests, xyloglucuronomannan in which the composition ratio of α-1,3-mannose and D-glucuronic acid has a predetermined value, in particular, a heterobasily categorized as a genus Tremella The present invention finds that xyloglucuronomannan extracted from a bacterial species has an action of maintaining or increasing useful enteric bacteria such as Bifidobacterium and Lactobacillus and suppressing Clostridium, which is an enteric harmful bacteria. Was completed.

  This invention is made | formed in view of the said knowledge, ie, it aims at finding the novel component which has an intestinal flora improvement effect and is safe.

The present invention has the characteristics described below.
1. The main chain is composed of α-1,3-mannose and contains xyloglucuronomannan having D-xylose and D-glucuronic acid as side chains,
The composition ratio [α-1,3-mannose: D-glucuronic acid] of α-1,3-mannose and D-glucuronic acid in the xylog chronomannan is 9: 1 to 9: 3.5 (moler The intestinal flora improving agent characterized by these.
2. The intestinal flora improving agent characterized in that the xyloglucuronomannan has a weight average molecular weight of 800000 to 1600000 calculated by a viscosity method.
3. The intestinal flora-improving agent according to claim 1 or 2, wherein the xyloglucuronomannan is extracted from a hetero-basidiomycete species classified as a genus Tremella.
4). Above 1. ~ 3. The pharmaceutical which uses the intestinal flora improving agent of any one of these as an active ingredient.
5). Above 1. ~ 3. Food / beverage products which use the intestinal flora improving agent of any one of these as an active ingredient.
6). Above 1. ~ 3. Cosmetics which use the intestinal flora improving agent of any one of these as an active ingredient.

Hereinafter, the present invention will be described in detail.
The intestinal flora improving agent of the present invention is characterized by comprising xylog chronomannan.
The “xyloglucuronomannan” is a general term for heteropolysaccharides having a main chain composed of α-1,3-mannose and having D-xylose and D-glucuronic acid as side chains.
The intestinal flora improving agent of the present invention has a composition ratio [α-1,3-mannose: D-glucuronic acid] of α-1,3-mannose and D-glucuronic acid of 9: 1 to 9: 3.5. (Moler), preferably 9: 1 to 9: 3 (moler). When the ratio is less than 9: 1 (moler) or exceeds 9: 3.5 (moler), the function of improving intestinal flora is inferior, which is not preferable.

  Further, the composition ratio of α-1,3-mannose and D-xylose is not particularly limited in the intestinal flora improving agent of the present invention, but the composition ratio [α-1,3-mannose: D-xylose] is 9: It can be set to 3 to 9: 6 (moler), preferably 9: 3.5 to 9: 6 (moler). If it is less than 9: 3 (moler) or exceeds 9: 6 (moler), the intestinal flora improving function is inferior, which is not preferable.

Further, the side chain of the above xylog chronomannan may have other sugars in addition to D-xylose and D-glucuronic acid, such as D-fucose, D-mannuronic acid, L-guluronic acid, etc. It may have at least one or more of them, and is not limited to these.
When D-fucose is included as a side chain, the composition ratio of α-1,3-mannose and D-fucose is not particularly limited, but the composition ratio [α-1,3-mannose: D-fucose] is 9: 0. 5-9: 4 (moler), preferably 9: 1-9: 4 (moler). When the ratio is less than 9: 0.5 (moler) or exceeds 9: 4 (moler), the function of improving intestinal flora is inferior, which is not preferable.

  Although the molecular weight of the said xylog chronomannan is not specifically limited, It is preferable that it is 700000-1600000 by the weight average molecular weight measured by the viscosity method, Preferably it is 750000-1300000, More preferably, it is 800000-1100000. If less than 700,000 or more than 1600000, the intestinal flora improving action is inferior, which is not preferable.

  The method for obtaining the intestinal flora-improving agent of the present invention is not particularly limited, but in the present invention, it can be produced by subjecting a hetero-basidiomycete species classified as a genus Tremella to extraction with water.

At this time, the kind of hetero-basidiomycetes classified in the genus Tremella (T.) is not particularly limited. T. fuciformis, T. et al. Mecentrica (T.
mesenterica), T .; T. aurantia, T. T. cinnabarina, and T. cinnabarina encepuala etc. are included, but are not limited to these. These may be used alone or in combination of two or more. Of these, T. Fushiformis (T.
fuciformis) is preferred. This is because the intestinal flora improving function is excellent.

  The site | part of the hetero-basidiomycetes species classified into the Tremella genus used by this invention is not specifically limited, For example, all of a mycelium, a fruit body, and yeast can be used. In addition, these can also use only 1 type of site | parts, and can also use 2 or more types site | part together.

When using hetero-basidiomycetes categorized as Tremella genus, the so-called raw state, its crushed material, its ground product, its dry product or its dry pulverized product can be used. As the mycelium in this state, in addition to the mycelium separated from the mycelium culture, the mycelium culture itself can be used.
The intestinal flora improving agent of the present invention can be obtained by adding water to these for extraction treatment. At this time, it is preferable to perform extraction with hot water. This is because extraction with hot water can increase the yield of the target polysaccharide-containing composite component. At this time, extraction may be performed while heating after adding water, or extraction may be performed while keeping warm by adding hot water from the beginning.
Specifically, 10 to 100 times the weight of water is added to a dry pulverized product of the hetero-basidiomycetes classified as Tremella, heated to 60 to 95 ° C., and stirred under reflux as necessary. By extracting for 0.5 to 10 hours, filtering or centrifuging, an extract can be obtained and this extract can be used as an intestinal flora improving agent. Moreover, the same extraction process may be repeated several times on the extraction residue, the extract obtained by this extraction process may be mixed and concentrated, and this concentrate may be used as an intestinal flora improving agent. In the intestinal flora improving agent, such an extract, a concentrated solution thereof, a dried product thereof or a dried pulverized product thereof can be used as an active ingredient.

Moreover, you may use the separated liquid which carried out the solid-liquid separation process of the mycelium culture of the hetero-basidiomycete species classified into Tremella genus as an intestinal flora improving agent of this invention. In addition, the liquid medium is inoculated not only with mycelium cultured by inoculating and culturing a hetero-basidiomycete species classified as a Tremella genus, but also in the culture solution corresponding to the above-mentioned separated solution. It is because the polysaccharide containing complex component which has a flora improvement effect is contained.
In the above-mentioned intestinal flora improving agent, in addition to the separated liquid, the concentrate, the dried product, or the dried pulverized product can be used as an active ingredient.

  Extract of fruiting body of hetero-basidiomycetes classified as Tremella obtained by the method described above, its concentrate, or its dried product, or mycelium separation liquid, its concentrated product, or its dried product Simply “Tremella extract”) is precipitated with a polar organic solvent to obtain a precipitate (hereinafter simply “Tremella extract purified product”), and this Tremella extract purified product is improved in intestinal flora. It is preferable to use as an active ingredient of the agent. This is because the Tremella extract also contains a low-molecular-weight water-soluble substance that does not have an action to improve the intestinal flora of the extract, and these can be removed by performing the precipitation treatment. At this time, various alcohols, acetone, and the like can be used as the polar organic solvent, but it is preferable to use a lower alcohol, particularly ethyl alcohol or isopropyl alcohol. This is because the residual organic solvent is taken into consideration and the yield of the target polysaccharide-containing composite component is increased. Specifically, 1 to 5 times the weight of ethyl alcohol is added to a 1/2 to 1/10 concentrated solution of the above extract or separation solution, and the mixture is stirred and then centrifuged to obtain a precipitate. .

  The above-mentioned intestinal flora improving agent can be blended in food and drink. In this case, there are no particular restrictions on the type of food or drink, and these can be used as additive materials for juices such as vegetable juice and fruit juice, and as additive materials for seasonings such as tomato ketchup and wooster sauce, and baked products such as bread and cookies. As an additive material for fried foods and soups, edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummi, tablet confectionery, etc.), noodles (Soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), general foods including soups, health foods (tablets, capsules, etc.), nutrition It can be used as an additive material for food supplements (such as nutritional drinks) and foods for swallowing.

These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. Furthermore, intestinal flora improving agents with health maintenance functions include other antioxidants and health food ingredients such as antioxidants, reduced ascorbic acid (vitamin C), vitamin E, and reduced forms. Glutatin, tocotrienol, vitamin A derivative, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechins, isoflavones, chalcones, tannins, Flavonoids, coumarins, isocoumarins, blueberry extract, arbutin, tannin, anthocyanin, apple polyphenol, grape seed extract, ellagic acid, kojic acid, surge extract health food material, V. (vitamin) A, V.B1, V. B2, V.B6, V.B12, VC, VD, VE, VP Choline, niacin, pantothenic acid, calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium , Heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, rainbow trout extract, suppon, licorice, kokushi, keihi, hawthorn, ginger, ganoderma , Psyllium, chamomile, chamomile, dandelion, hibiscus, honey, bowlen, royal jelly, lime, lavender, rose hip, rosemary, sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil, Soybean oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DPA, green tea, cordyceps, garlic, bee, papaya, puer, propolis, Meguri Tree, Yabushake, Royal Jelly, Saw Palmetto, Hyaluronic Acid, Collagen, Gabba, Harp Seal Oil, Shark Cartilage, Glucosamine, Lecithin, Phosphatidylserine, Seven Carrots, Mulberry Leaf, Soybean Extract, Echinacea, Ezoukogi, Barley Extract , Olive leaf, olive fruit, Gymnema, Banaba, Salacia, Garcinia, Chitosan, St. John's wort, jujube, carrot, passion flower, broccoli, placenta, pearl barley, grape seeds, peanut seed coat, bilberry, black cohosh, maria Zami, laurel, sage, rosemary, raffma, black vinegar, bitter gourd, maca, safflower, flax, oolong tea, flower spine, caffeine, capsaicin, xylooligosaccharide, glucosamine, buckwheat, citrus, dietary fiber, protein, prunes, spirulina, Barley young leaves, nucleic acids, yeast, shiitake mushrooms, plum meat, amino acids, deep sea potato extract, noni, oyster meat, suppon, champignon, plantain, acerola, pineapple, banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, Meshimakobu, cranberry, chondroitin sulfate, zinc, iron, ceramide, silk peptide, glycine, niacin, chesttree, ceramide, L-cysteine, L-carnitine, red wine leaf, millet, horsetail, biotin, centella asiatica, hascup, Pycnogenou Lemon, Fuuki, Rhubarb, Clove, Rosemary, Catechin, Pual, Citric Acid, Beer Yeast, Merirot, Black Zinger, Ginger, Gajutsu, Nattokinase, Benikouji, Tocotrienol, Lactoferrin, Cinnamon, Persimmon Buckwheat, Cocoa, Yuzu Seed Extract, Perilla Berry extract, lychee seed extract, evening primrose extract, black rice extract, α-lipoic acid, gabba, fresh coffee bean extract, buffalo extract, kiwi seed extract, Unshu mandarin orange extract, red ginger extract, astaxanthin) and the like.

  As a specific production method, the intestinal flora improving agent of the present invention is spray-dried or freeze-dried as it is or together with powdered dextrin, and this is easily converted into a powder, granule, tableting or solution to produce food (instant food etc. ). If necessary, it can also be mixed with a binder such as gum arabic to form a powder or granules, and added to a solid food. Moreover, it is also possible to add to a beverage as it is or after being dispersed and dissolved in water, ethanol, glycerin or a mixture thereof.

  The intestinal flora improving agent of the present invention may be used as a raw material for drugs (including pharmaceuticals and quasi drugs). It can be produced by appropriately blending the intestinal flora improving agent of the present invention with a raw material for a pharmaceutical preparation. In addition, the said chemical | medical agent may be used for a human and may be used for mammals other than a human. Examples of the preparation raw material that can be blended in the intestinal flora improving agent of the present invention include, for example, excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin, Talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, carbonate) Sodium hydride, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (No. Quaternary ammonium base, sodium lauryl sulfate Etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

  The administration method of the intestinal flora improving agent according to the present invention can generally be orally administered in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules and the like. The water-soluble preparation can be administered orally as a liquid. Furthermore, parenteral administration may be used. When administered as a parenteral agent, the intestinal flora improving agent of the present invention is dispersed in an appropriate solubilizing agent such as ethanol or water, and then an agent such as a poultice, lotion, ointment, tincture or cream. Can be applied in form. Intestinal flora-improving agents should be applied as they are or in the form of poultices, lotions, ointments, tinctures, creams, etc. with the addition of dispersants, suspensions, stabilizers, etc. Can do.

  The dosage may vary depending on the administration method, medical condition, age of the patient, etc., but for adults, it can usually be administered as an active ingredient per day from 5 to 400 mg, and for children, usually from about 0.5 to 200 mg.

  The compounding ratio when the intestinal flora improving agent of the present invention is used as a drug can be appropriately changed depending on the dosage form, but is usually about 0.01 to 10 wt.% When administered orally or by mucosal absorption. It should be about%. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range. In addition to the intestinal flora-improving agent, the pharmaceutical composition may include other known compounds commonly used in the pharmaceutical field and compounds necessary for molding into a form suitable for oral administration. Examples of such compounds include lactose, starch, hydroxypropyl cellulose, kaolin, talc, calcium carbonate and the like.

Moreover, the intestinal flora improving agent of this invention can be contained in the animal feed for mammals. The animal feed can be contained in the same manner as the food and drink. The animal feed is not particularly limited. For example, it is used for domestic animals such as cattle and pigs, companion animals such as dogs, cats and hamsters (animals kept as pets). can do.
For example, flour, meat, etc. can be used as companion animal feed. At this time, examples of the flour include wheat flour, rice flour, rye flour, corn flour, millet flour, bubble flour, corn flour, and soybean flour, and these flours may be used in combination of two or more. By using flour, nutrients such as carbohydrates can be supplied to companion animals. Among the above flours, it is most preferable to use flour, and as flour, strong flour, medium flour, thin flour can be used alone or in combination, and even when such flour and other flour are used in combination Good.

Even if the intestinal flora improving agent of the present invention is used as an external preparation for skin (including cosmetics, pharmaceuticals and quasi drugs), an intestinal flora improving action can be expected.
This is because the intestinal flora improving agent of the present invention can exert an intestinal flora improving action in the presence of an acid by being used as a skin external preparation for cosmetics or the like. In addition, the said skin external preparation may be used for a human and may be used for mammals other than a human.
Examples of the external preparation for skin to which the intestinal flora improving agent of the present invention can be blended include, for example, emulsion, soap, face wash, bath preparation, cream, emulsion, lotion, eau de cologne, shave cream, shave lotion. , Cosmetic oil, tan / sunscreen lotion, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, Rinse, hair dye, dispersion liquid, cleaning agent and the like can be mentioned.
Examples of the form of a pharmaceutical or quasi-drug that can contain the intestinal flora improving agent of the present invention include ointments, creams, and liquids for external use.

In addition to the intestinal flora-improving agent according to the present invention, the external preparation for skin of the above-described form includes components, oils and the like that are blended in external preparations for skin such as cosmetics and quasi-drugs as long as the intestinal flora-improving action is not impaired. Higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, and the like.
Examples are listed below, but the present invention is not limited to these examples.

(1) Examples of oil components Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Isocetyl, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoaralkyl neopentanoate, glyceryl tri (capryl / capric acid), trimethylolpropane tri-2-ethylhexanoate, trimethylol triisostearate Propane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethyleneglycol dioctanoate , Ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, triiso Glyceryl palmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin olein Acid ester, polyglycerol isostearate, dipropyl carbonate, charcoal Dialkyl (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, olein Cholesteryl acid, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoyl hydroxystearate Cetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
Hydrocarbon oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, kiwi seed oil, apricot oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil , Perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil , Vegetable oils such as sunflower oil, grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened , Beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethyl Siloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic Examples include silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.

(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.

(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono-2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, diisopropyl diisopropylcinnamic acid Stealth mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, Butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole , Methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3, 3-diphenyl acrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) can Examples include full, isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2, 5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, polymer derivatives, silane derivatives, and the like.

(5) Examples of powders and pigments Red 104, Red 201, Yellow 4, Blue 1, Black 401 and other dyes, Yellow 4 AL lake, Yellow 203 BA lake and other lake dyes, nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, metal salts such as magnesium silicate, Inorganic powders such as Rica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite, boron nitride, etc. It is done. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.

(6) Examples of surfactants Anionic surfactant: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate , Alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetic acid Sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, etc. are mentioned.
Cationic surfactant: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like.
Amphoteric surfactants: carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant: Propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbit fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
Natural surfactant: lecithin, saponin, sugar surfactant and the like.

(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1, 3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.

(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , BASF), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubicol VAP, BASF), vinyl acetate / crotonic acid copolymer (Rubiset CA, BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (Rubiset CAP, manufactured by BASF), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BA SF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxy vinyl polymer (Carbopol, BF Goodrich), anionic polymer compounds such as acrylic acid / alkyl methacrylate copolymers (Pemulene, BF Goodrich) and acetic acid amphoteric compounds of dialkylaminoethyl methacrylate polymers (Yukaformer, Mitsubishi Chemical) Amphoteric polymer compounds such as octyl acrylate acrylate / hydroxypropyl acrylate / butyl aminoethyl methacrylate copolymer (AMPHOMER, NSC), quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP) , Methyl vinyl imidazolium chloride Cationic polymer compounds such as vinyl / vinylpyrrolidone copolymer (rubicoat, manufactured by BASF), polyvinylpyrrolidone (rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (rubiscol VA, manufactured by BASF) ), Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP), etc. There is. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.

(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, immunostimulants, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, A cooling sensation agent, a warming sensation agent, vitamins, an amino acid, a wound healing promoter, an irritation relaxation agent, an analgesic agent, a cell activator, an enzyme component, etc. are mentioned. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, Carrot extract, Kawara mugwort extract, Licorice extract, Calcade extract, Oyster extract, Kina extract, Cucumber extract, Guanosine, Gardenia extract, Kumazasa extract, Clarae Kiss, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, tea extract, yeast extract, burdock extract, fermented rice bran extract, rice germ oil, comfrey extract, collagen, bilberry extract, saicin extract, psycho extract Extract Kizuta extract, hawthorn extract, elderberry extract, yarrow extract, mint extract, sage extract, mallow extract, senki Sue extract, assembly extract, soybean extract, tiso extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, toshinsen extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, Novara extract, hibiscus extract, bacmond extract, parsley extract, honey, hamamelis extract, parietalia extract, toad extract, bisabolol, loquat extract, dandelion extract, burdock extract, bukuro extract, butcher bloom extract, grape extract, propolis, loofah extract, Safflower extract, peppermint extract, bodaige extract, button extract, hop extract, pine extract, marronnier extract, mizubasho Kiss, Mukuroji extract, Melissa extract, Peach extract, Cornflower extract, Eucalyptus extract, Yukinoshita extract, Yokuinin extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Lotus root extract, Rose extract, Rosemary extract, Roman chamomile extract And royal jelly extract.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Immunostimulants such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester Vitamins, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoid, flavonoid, tannin, lignan, saponin, α-hydroxy acid, β-hydroxy acid, etc. Cell activators, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione, etc. Whitening agent, cephalanthin, licorice extract, red pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pan Tothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, pyroctone Olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantalis tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, monostearic acid Examples include hair growth agents such as polyoxyethylene sorbitan, mint oil, and Sasanishiki extract.

(10) Examples of antioxidants Sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolyl biguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.

Example: Preparation of intestinal flora improving agent The fruiting body of T. fuciformis was washed, added with 50 times weight of water, heated to 60-95 ° C., and extracted for 5 hours while stirring under reflux as necessary. Then, it filtered or centrifuged and obtained the extract. Five times the weight of ethyl alcohol was added to this extract, stirred and then centrifuged to obtain an intestinal flora improving agent (Example). When this intestinal flora improving agent was analyzed by the IR spectrum method, the intestinal flora improving agent of this example was composed of α-1,3-mannose as the main chain, and D-fucose, D as side chains. -Heteroside having xylose and D-glucuronic acid, with a composition ratio [α-1,3-mannose: D-fucose: D-xylose: D-glucuronic acid] in a molar ratio of 9: 1: 4: 3 It was confirmed to be a polysaccharide, that is, xylog chronomannan. Furthermore, when the weight average molecular weight was computed by the viscosity method, it was confirmed that it was about 1000000.

Test example: Evaluation test method for intestinal flora improving action Six in-house male volunteers were allowed to freely take the intestinal flora improving agent (50 mg) of this example once a day for 3 weeks. Then, before ingestion of the intestinal flora improving agent and after free intake of this example for 3 weeks, stool Bifidobacterium spp., Lactobacillus spp., Clostridium (C.) perfringens)
E. coli (Escherichia coli), and bacteriological examination of lactose degradation coliforms (the dilution factor 10 ^more) was carried out by the following method. The results are shown in FIG. 1 (Bifidobacterium spp.), FIG. 2 (Lactobacillus spp), FIG. 3 (C. perfringens), FIG. 4 (Escherichia coli), and FIG. 5 (lactose-degrading coliforms).

1-1 Bacteria Test Method 1) Measurement of Bifidobacterium spp., Lactobacillus spp., Clostridium (C.) perfringens 1 g of sample (stool) was collected and 9 ml of physiological saline To obtain a physiological saline sample solution. This saline sample solution is diluted to 10 ² , 10 ³ , 10 ⁴ , 10 ⁵ , 10 ⁶ , 10 ⁷ , 10 ⁸ , and 10 ⁹ to prepare diluted solutions, and each diluted solution is transferred to an anaerobic agar medium. Was anaerobically cultured at 37 ° C. for 5 days.
Then, the number of bacteria was calculated based on the number of colonies generated in the medium after culturing each diluted solution. The results are shown in FIG. 1 (Bifidobacterium spp.), FIG. 2 (Lactobacillus spp), and FIG. 3 (C. perfringens).

2) Measurement of Escherichia coli and lactose-degrading coliforms 1 g of a sample (stool) was collected and mixed with 9 ml of physiological saline to obtain a physiological saline sample solution. This physiological saline sample solution is diluted to 10 ² , 10 ³ , 10 ⁴ , 10 ⁵ , 10 ⁶ , 10 ⁷ , 10 ⁸ , 10 ⁹ to prepare a diluted solution, and each diluted solution is transferred to an aerobic agar medium. Each was aerobically cultured at 37 ° C. for 5 days.
Then, the number of bacteria was calculated based on the number of colonies generated in the medium after culturing each diluted solution. The results are shown in FIG. 4 (Escherichia coli) and FIG. 5 (lactose-degrading coliforms).

Results and Effects of Examples According to FIGS. 1 to 3, the number of Bifidobacterium (Bifidobacterium spp., See FIG. 1) and Lactobacillus spp. (See FIG. 2) bacteria when this example is ingested for 3 weeks. It can be seen that there is a tendency for Clostridium (C.) perfringens to further decrease. Thereby, it turns out that it has the effect | action which increases intestinal useful bacteria and decreases pathogenic bacteria in a present Example. Thereby, it turns out that there exists an effect | action which improves an intestinal flora in a present Example.
Moreover, according to FIG. 4, by taking this example, Escherichia coli, which is one of the intestinal useful bacteria, has a value closer to the reference value (10 ¹¹ CFU / g) existing in the intestine. Show. Furthermore, according to FIG. 5, it can be seen that the total number of bacteria in the lactose-degrading coliform group is increasing. Thereby, it turns out that a present Example has a prebiotic action.

Formulation Examples Hereinafter, formulation examples of the intestinal flora improving agent of the present invention will be described, but the following formulation examples do not limit the present invention.
Formulation Example 1: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Intestinal flora improving agent 0.5
100.0wt%

Formulation Example 2: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Grape juice 4.0
Grape flavor 0.6
Dye 0.02
Intestinal flora improving agent 1.0
100.0wt%

Formulation Example 3: Candy
50.0 wt% sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Intestinal flora improving agent 0.4
100.0wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Intestinal flora improving agent 0.4
Perfume
Water residue
100.0wt%

Formulation Example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Intestinal flora improving agent 0.05
Perfume
Purified water residue
100.0wt%

Formulation Example 6: Tablets
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Intestinal flora improving agent 0.5
Purified water 3.9
100.0wt%

Formulation Example 7: Soft capsule
Grape seed oil 87.0wt%
Emulsifier 12.0
Intestinal flora improving agent 1.0
100.0wt%

Formulation Example 8: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Intestinal flora improving agent 1.0
100.0wt%

Formulation Example 9: Oral granules (pharmaceuticals)
Intestinal flora improving agent 1.0wt%
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 8.0
Polyvinylpyrrolidone 1.0
100.0wt%
Formulation Example 10: Food for swallowing
Intestinal flora improving agent 1.0wt%
Dextrin 60.0
Starch 39.0
100.0wt%

Formulation Example 11: Cosmetic cream
Squalane 20.0wt%
Beeswax 5.0
Refined jojoba oil 5.0
Glycerin 5.0
Glycerol monostearate 2.0
Polyoxyethylene (20) sorbitan-
Monostearate 2.0
Intestinal flora improving agent 2.0
Preservative appropriate amount
Perfume
Purified water residue
100.0wt%

Formulation Example 12: lotion
Ethanol 5.0wt%
Glycerin 2.0
1,3-butylene glycol 2.0
Polyethylene oleyl ether 0.5
Sodium citrate 0.1
Citric acid 0.1
Intestinal flora improving agent 0.1
Purified water residue
100.0wt%

Formulation Example 13: Body gel
Macadamia nut oil 2.0wt%
Octyldodecyl myristate 10.0
Methylphenylpolysiloxane 5.0
Behenyl alcohol 3.0
Stearic acid 3.0
Batyl alcohol 1.0
Glyceryl monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit 2.0
Hydrogenated soybean phospholipid 1.0
Ceramide 0.1
Retinol palmitate 0.1
Preservative appropriate amount
Centella extract 1.0
Intestinal flora improving agent 1.0
1,3-butylene glycol 5.0
Purified water residue
100.0wt%

Formulation Example 14: Emulsion
Squalane 4.0wt%
Vaseline 2.5
Cetanol 2.0
Glycerin 2.0
Lipophilic glyceryl monostearate 1.0
Stearic acid 1.0
L-Arginine 1.0
Intestinal flora improving agent 0.5
Potassium hydroxide 0.1
Perfume
Purified water residue
100.0wt%

Formulation Example 15: Bath agent (liquid)
Propylene glycol 50.0wt%
Ethanol 20.0
Sodium sulfate 5.0
Intestinal flora improving agent 0.5
Lanolin 0.5
Avocado oil 0.5
Dye 1.5
Fragrance 22.0
100.0wt%

Formulation Example 16: Cat food
Corn 34.0wt%
Flour 35.0
Meat meal 15.0
Beef tallow 8.9
Salt 1.0
Skipjack extract 4.0
Intestinal flora improving agent 1.0
Taurine 0.1
Vitamins 0.5
Minerals 0.5
100.0wt%

Formulation Example 17: Dog food
Corn 30.0wt%
Meat (chicken) 15.0
Defatted soybean 10.0
Flour 25.0
Mosses 5.0
Intestinal flora improving agent 5.0
Animal fats and oils 8.9
Oligosaccharide 0.1
Vitamin 0.5
Mineral 0.5
100.0wt%

  As described above, the present invention can provide an intestinal flora-improving agent comprising a safe novel ingredient as an active ingredient, and a pharmaceutical, a food or drink, and a skin external preparation using the same.

It is a graph which shows the change of the number of bifidobacterium (Bifidobacterium spp.) Before ingesting a present Example and after ingesting for 3 weeks. It is a graph which shows the change of the number of lactobacillus (Lactobacillus spp.) Before ingesting a present Example and after ingesting for 3 weeks. It is a graph which shows the change of the number of the Clostridium perfringens [Clostridium (C.) perfringens] before ingesting a present Example and after ingesting for 3 weeks. It is a graph which shows the change of the number of colon_bacillus | E._coli (Escherichia coli) before ingesting a present Example and after ingesting for 3 weeks. It is a graph which shows the change of the number of lactose-degrading coliform bacteria before ingesting this Example and after ingesting for 3 weeks.

Claims (6)

The main chain is composed of α-1,3-mannose and contains xyloglucuronomannan having D-xylose and D-glucuronic acid as side chains,
The composition ratio [α-1,3-mannose: D-glucuronic acid] of α-1,3-mannose and D-glucuronic acid in the xylog chronomannan is 9: 1 to 9: 3.5 (moler The intestinal flora improving agent characterized by these. The intestinal flora improving agent characterized in that the xyloglucuronomannan has a weight average molecular weight of 800000 to 1600000 calculated by a viscosity method.   The intestinal flora-improving agent according to claim 1 or 2, wherein the xyloglucuronomannan is extracted from a hetero-basidiomycete species classified as a genus Tremella.   The pharmaceutical which uses the intestinal flora improving agent of any one of Claims 1-3 as an active ingredient.   Food-drinks which use the intestinal flora improving agent of any one of Claims 1-3 as an active ingredient. Cosmetics which use the intestinal flora improving agent of any one of Claims 1-3 as an active ingredient.

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