JP2010270021A - Ucp gene expression-promoting agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a UCP gene expression-promoting agent containing a new ingredient as an active ingredient. <P>SOLUTION: This UCP gene expression-promoting agent contains fucoxanthin as an active ingredient. The UCP gene expression-promoting agent may contain the extract of at least one selected from algae containing fucoxanthin as an active ingredient. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

The present invention relates to an expression promoter for genes related to lipid metabolism.

The energy balance of the whole living body is maintained by a signal based on excess or deficiency of body energy being transmitted to the center (hypothalamus), and automatic adjustment of energy intake (feeding behavior) and consumption. If the regulatory mechanism fails, it becomes obese. For example, if you continue to eat voluntarily, energy consumption increases adaptively, and conversely decreases under fasting. Most obese animals have reduced energy consumption, and obesity cannot be resolved by restricting food intake to the same amount as normal animals. One of the promising candidate molecules involved in such autonomous regulation of energy consumption is mitochondrial uncoupling protein (UCP).
UCP
Uncouples oxidative phosphorylation in the inner mitochondrial membrane and dissipates energy as heat. For the most representative brown adipose tissue UCP (UCP1): 1. UCP1 function is reduced in obese animals. 2. UCP1 is increased in animals that are not obese even after multiple diets. It is known that mice that have artificially reduced the expression of UCP1 are obese and mice that are highly expressing can lose weight. Therefore, when UCP1 is activated, an anti-obesity effect can be expected. Therefore, drugs and foods for this purpose are being searched for. A typical example is an adipocyte-specific β3 adrenergic receptor agonist. In fact, β3 agonists promote lipolysis in white adipocytes and at the same time activate UCP1 to convert the released fatty acids into heat, ultimately reducing body fat.

  Under such a background, the present inventors have found that fucoxanthin, which is a type of carotenoid, and an algal extract containing the same have an action of promoting the expression of the UCP gene.

The present invention has been made to solve the above problems, and an object of the present invention is to provide a UCP gene expression promoter containing a novel component as an active ingredient.

The features of the present invention for solving the above-described problems are as follows.
1. UCP gene expression promoter containing fucoxanthin as an active ingredient.
2. A UCP gene expression promoter comprising as an active ingredient at least one extract selected from algae containing fucoxanthin.

It is a graph which shows the result of the mRNA expression promotion effect | action of UCP-1 of the fucoxanthin containing kelp extract of a present Example. It is a graph which shows the result of the mRNA expression promotion effect | action of UCP-2 of the fucoxanthin containing kelp extract of a present Example.

Hereinafter, the present invention will be described in detail.
The present invention is characterized by containing fucoxanthin as an active ingredient.
Fucoxanthin is a known substance whose chemical formula is known as follows, and a substance with high purity has an orange columnar crystal structure.

The method for obtaining the fucoxanthin is not particularly limited, but it is preferably extracted from a natural product. This is because more stable fucoxanthin can be obtained.
Moreover, when obtaining fucoxanthin from a natural product, the raw material is not specifically limited, For example, algae etc. are mentioned.

The “algae” is not particularly limited, and for example, brown algae, red algae, green algae, diatoms and the like can be used, and among these, brown algae are particularly preferable. This is because they contain a relatively large amount of fucoxanthin, and are abundant as resources and industrially contribute as raw materials. These may be used alone or in combination of two or more.

Examples of the “brown algae” include, for example, Laminariaceae, Chordaceae, Chamaceae (Laminariales), Fumaceae, Sargasaceae, etc. More preferred are, but not limited to, brown algae belonging to the order of Chlordiales, such as Chordaceae and Permatochaceae. Furthermore, among these, it is particularly preferable to use a comb. These may be used alone or in combination of two or more.
In addition, when using the kombu as a raw material, it is preferable to use a macaque of the family Kombu, onicomb, resilibu, hosocomb, honeycomb, nagacombu, gagome, wakame of the chigaiso family, and the like. This is because fucoxanthin is contained at a higher concentration. These may be used alone or in combination of two or more.
In addition, the said algae may use 1 type of extracts of these, and may use 2 or more types of extracts together.

The extraction method for obtaining fucoxanthin from the algae is not particularly limited, and examples thereof include polar solvent extraction and supercritical extraction. Only one of these may be performed, or both of them may be performed. Moreover, this extraction may be performed only once each method, or may be performed twice or more.

Here, when extracting by polar solvent extraction, the polar solvent to be used is not particularly limited. For example, water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, Examples include ethyl acetate, ether, hexane and the like. Of these, water, methanol, and ethanol are preferred. It is because an active ingredient can be extracted efficiently. These may be used alone or in combination of two or more.

When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C, preferably about 40 to 70 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used.

When hydrous alcohol is used as the extraction solvent, the alcohol concentration is preferably 30 wt% or more, and preferably 50 wt% or more. It is because it is difficult to obtain a high extraction amount of an active ingredient when it is less than 30 wt%. The extraction temperature is 0 to 95 ° C, preferably about 20 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  In addition, when extracting with a polar solvent, the extraction method is not particularly limited, and for example, any method such as continuous extraction, immersion extraction, countercurrent extraction, etc. can be employed, and any device can be used at room temperature or under reflux heating. Can be used. In addition, when extracting by the method mentioned above, only one of these may be performed and these methods may be combined. These extractions may be performed only once or may be performed twice or more.

As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or a hydrous alcohol is used as the extraction solvent, the extraction is performed for about 1 minute to 150 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying, and purification according to a conventional method to obtain an extract containing a high concentration of fucoxanthin.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like.

  Furthermore, when performing extraction by supercritical extraction, the supercritical fluid used at this time is not particularly limited, and examples thereof include carbon dioxide and nitrogen. In addition, these may use only 1 type and may use 2 or more types together. Of these, carbon dioxide is particularly preferred. It is because an active ingredient can be extracted more easily. The extraction method at this time may be performed by a known method. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying, and purification according to a conventional method to obtain an extract containing a high concentration of fucoxanthin.

  The present invention provides a food containing the UCP gene expression promoter. This food may contain the extract as an active ingredient in an amount of 100 to 1,000 mg, preferably 250 to 500 mg, for example, per adult dose. In the UCP gene expression promoter of the present invention, the content of the active ingredient may vary depending on age and the like.

  The UCP gene expression promoter of the present invention can be used as a material for various foods and drinks. Examples of food and drink include edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummy, tablet confection etc.), noodles (soba, udon, ramen etc.), dairy products ( Milk, ice cream, yogurt, etc.), boiled simmered (simmered in kombu simmered, paste simmered in sea bream, small fish simmered, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated beverages, sports General foods such as beverages), health foods (tablets, capsules, etc.), and nutritional supplements (nutrient drinks, etc.). The UCP gene expression promoter of the present invention may be appropriately blended with these foods and drinks.

These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. Furthermore, this UCP gene expression promoter with health maintenance function includes other antioxidants and health food ingredients such as antioxidants, reduced ascorbic acid (vitamin C), vitamin E, reduced Glutatin, tocotrienol, vitamin A derivative, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechins, isoflavones, chalcones, tannins , Flavonoids, coumarin, isocoumarins, blueberry extract, arbutin, tannin, anthocyanin, apple polyphenol, grape seed extract, ellagic acid, kojic acid, surge extract health food material, V. (vitamin) A, V.B1, V .B2, V.B6, V.B12, VC, VD VE, VP, choline, niacin, pantothenic acid, calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium , Selenium, potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kukoshi, kehi, hawthorn, ginger, ganoderma, swordfish extract, suppon, plantain, chamomile, chamomile, dandelion, Hibiscus, honey, Boren, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium chain fatty acids, agaricus, i Leopard leaf extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DPA, green tea, cordyceps, garlic, bee puppy, papaya, puer, propolis, measli tree, yabushitake, royal jelly, saw palmetto, Hyaluronic acid, collagen, gabba, harp seal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, paddy carrot, mulberry leaves, soy extract, echinacea, elegans, barley extract, olive leaf, olive fruit, gymnema, banaba, Salacia, Garcinia, Chitosan, St. John's wort, jujube, carrot, passion flower, broccoli, placenta, pearl barley, grape seed, peanut seed coat, bilberry, black cohosh, maria thistle, laurel, sage, rosemary, rafma , Black vinegar, bitter gourd, maca, safflower, flax, oolong tea, flower spine, caffeine, capsaicin, xylo-oligosaccharide, glucosamine, buckwheat, citrus, dietary fiber, protein, prunes, spirulina, barley young leaves, nucleic acid, yeast, shiitake mushroom, plum Meat, amino acid, deep sea bream extract, noni, oyster meat, suppon, champignon, plantain, acerola, pineapple, banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, mesimacob, cranberry, chondroitin sulfate, zinc, iron , Ceramide, Silk Peptide, Glycine, Niacin, Chest Tree, Ceramide, L-Cysteine, L-Carnitine, Red Wine Leaf, Millet, Horsetail, Biotin, Centella Asiatica, Hascup, Pycnogenol, Fuki, Rhubarb, Clove, Rosemary , Catechin, puer, citric acid, brewer's yeast, merirot, black zinger, ginger, gadget, nattokinase, benicouji, tocotrienol, lactoferrin, cinnamon, buckwheat, cocoa, yuzu seed extract, perilla seed extract, litchi seed extract, evening primrose extract , Black rice extract, α-lipoic acid, gabba, green coffee bean extract, burdock extract, kiwi seed extract, Unshu mandarin orange extract, red ginger extract, astaxanthin) and the like.

As a specific production method, the UCP gene expression promoter of the present invention is used as it is, and in the case of an extract, it is spray-dried or freeze-dried together with powdered dextrin, and this is made into a powder, granule, tablet, or solution for easy food production. (Instant food, etc.) If necessary, it can also be mixed with a binder such as gum arabic to form a powder or granules, and added to a solid food. In the case of an extract, it can also be added to a beverage as it is or after being dispersed and dissolved in water, ethanol, glycerin or a mixture thereof.

The UCP gene expression promoter of the present invention may be used as a raw material for drugs (including drugs and quasi drugs). It can be produced by appropriately blending the UCP gene expression promoter of the present invention with a raw material for a pharmaceutical preparation. In addition, the said chemical | medical agent may be used for a human and may be used for mammals other than a human. Examples of the pharmaceutical raw material that can be blended in the UCP gene expression promoter of the present invention include, for example, excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin, Talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, carbonate) Sodium hydride, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (No. Quaternary ammonium base, sodium lauryl sulfate Um, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

In general, the UCP gene expression promoter according to the present invention can be administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules and the like. The water-soluble preparation can be administered orally as a liquid. Furthermore, parenteral administration may be used. When administered as a parenteral agent, the UCP gene expression promoter of the present invention is dispersed in an appropriate solubilizing agent such as ethanol or water, and then an agent such as a poultice, lotion, ointment, tincture or cream. Can be applied in form. In addition, the water-soluble preparation of this UCP gene expression promoter is an agent such as a poultice, lotion, ointment, tincture, cream, etc., as it is or with a dispersant, suspension, stabilizer, etc. added. Can be applied in form.

The dose may vary depending on the administration method, medical condition, age of the patient, etc., but for adults, it can be generally administered in an amount of about 5 to 200 mg as an active ingredient per day and usually about 0.5 to 100 mg for children.

The compounding ratio when using the UCP gene expression promoter of the present invention as a drug can be appropriately changed depending on the dosage form, but is usually about 0.0001 to 30 wt% when administered orally or by mucosal absorption. %, In the case of parenteral administration, it may be about 0.0001 to 30 wt%. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range. In addition to the UCP gene expression promoter, the pharmaceutical composition may include other known compounds that are commonly used in the pharmaceutical field and compounds that are necessary for molding into a form suitable for oral administration. Examples of such compounds include lactose, starch, hydroxypropyl cellulose, kaolin, talc, calcium carbonate and the like.

Moreover, the UCP gene expression promoter of the present invention can be contained in mammalian animal feed. The animal feed can be contained in the same manner as the food and drink. The animal feed is not particularly limited. For example, it is used for domestic animals such as cattle and pigs, companion animals such as dogs, cats and hamsters (animals kept as pets). can do.
For example, flour, meat, etc. can be used as companion animal feed. At this time, examples of the flour include wheat flour, rice flour, rye flour, corn flour, millet flour, bubble flour, corn flour, and soybean flour, and these flours may be used in combination of two or more. By using flour, nutrients such as carbohydrates can be supplied to companion animals. Among the above flours, it is most preferable to use flour, and as flour, strong flour, medium flour, thin flour can be used alone or in combination, and even when such flour and other flour are used in combination Good. Furthermore, in order to adjust the elasticity of the animal feed after the heat treatment, wheat flour, wheat gluten, soybean protein and the like may be combined. In addition, the network structure derived from gluten contained in wheat flour can constitute a puffed tissue structure when heat-treated, which contributes to improving the texture.

  The meat used in the present invention is not particularly limited, and chicken, pork, beef, mutton, goat meat, salmon, turkey meat, horse meat, etc. can be used, but chicken is preferably used from the point of flavor. The The above meat is obtained by slaughtering and demolishing livestock in the usual way. In addition, since the deterioration of the quality of a product with intermediate moisture or low moisture is mainly caused by fat oxidation, the meat to be used is preferably red meat from which the fat content is low or fat is removed. In addition, the coexistence of meat can improve the palatability of companion animals as well as strengthening high-quality animal proteins.

Even if the UCP gene expression promoter of the present invention is used as an external preparation for skin (including cosmetics, pharmaceuticals and quasi drugs), it can be expected to promote UCP gene expression. In addition, the said skin external preparation may be used for a human and may be used for mammals other than a human.
Examples of the external preparation for skin to which the UCP gene expression promoting agent of the present invention can be formulated include, for example, emulsion, soap, facial cleanser, bath preparation, cream, emulsion, lotion, eau de cologne, shaving cream, shaving lotion. , Cosmetic oil, tan / sunscreen lotion, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, Rinse, hair dye, dispersion liquid, cleaning agent and the like can be mentioned.
In addition, examples of the form of a pharmaceutical or quasi-drug that can contain the UCP gene expression promoter of the present invention include ointments, creams, and liquids for external use.

In addition to the UCP gene expression promoting agent according to the present invention, the external preparation for skin of the above-described form includes ingredients, oil components, and the like blended in skin external preparations such as cosmetics and quasi-drugs as long as the UCP gene expression promoting effect is not impaired. Higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, and the like.
Examples are listed below, but the present invention is not limited to these examples.

(1) Examples of oil components Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Isocetyl, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoaralkyl neopentanoate, glyceryl tri (capryl / caprate), trimethylolpropane tri-2-ethylhexanoate, trimethylol triisostearate Propane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethyleneglycol dioctanoate , Ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, triisopalmitine Glyceryl acid, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl decyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleic acid Ester, polyglycerol isostearate, dipropyl carbonate, charcoal Dialkyl (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, olein Cholesteryl acid, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoyl hydroxystearate Cetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
Hydrocarbon oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, kiwi seed oil, apricot oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil , Perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil , Vegetable oils such as sunflower oil, grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened , Beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethyl Siloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic Examples include silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.

(2) Examples of higher alcohols include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.

(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono-2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, diisopropyl diisopropylcinnamic acid Stealth mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, Butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole , Methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3, 3-diphenyl acrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) can Le, isopropyl dibenzoylmethane, 4- (3,4-dimethoxyphenyl methylene) 2-ethylhexyl-2,5-dioxo-1-imidazolidin propionate,, and polymer derivatives and silane derivatives thereof.

(5) Examples of powders and pigments Red 104, Red 201, Yellow 4, Blue 1, Black 401 and other dyes, Yellow 4 AL lake, Yellow 203 BA lake and other lake dyes, nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, metal salts such as magnesium silicate, Inorganic powders such as Rica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite, boron nitride, etc. It is done. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.

(6) Examples of surfactants Anionic surfactants: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate , Alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetic acid Sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, etc. are mentioned.
Cationic surfactant: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like.
Amphoteric surfactants: carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant: propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
Natural surfactant: lecithin, saponin, sugar surfactant and the like.

(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1, 3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.

(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , BASF), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubicol VAP, BASF), vinyl acetate / crotonic acid copolymer (Rubiset CA, BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (Rubiset CAP, manufactured by BASF), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BAS) F), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BF Goodrich), anionic polymer compounds such as acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Goodrich), and acetic acid amphoteric products of dialkylaminoethyl methacrylate polymers (Yukaformer, Mitsubishi Chemical) Amphoteric polymer compounds such as octyl acrylate acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, NSC), quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP) , Methyl vinyl imidazolium chloride / Cationic polymer compounds such as vinylpyrrolidone copolymer (rubicoat, manufactured by BASF), polyvinylpyrrolidone (rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (rubiscol VA, manufactured by BASF) Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP), etc. is there. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.

(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, immunostimulants, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, A cooling sensation agent, a warming sensation agent, vitamins, an amino acid, a wound healing promoter, an irritation relaxation agent, an analgesic agent, a cell activator, an enzyme component, etc. are mentioned. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, Carrot extract, Kawara mugwort extract, Licorice extract, Calcade extract, Oyster extract, Kina extract, Cucumber extract, Guanosine, Gardenia extract, Kumazasa extract, Clarae Kiss, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, tea extract, yeast extract, burdock extract, fermented rice bran extract, rice germ oil, comfrey extract, collagen, bilberry extract, saicin extract, psycho extract Extract Kizuta extract, hawthorn extract, elderberry extract, yarrow extract, mint extract, sage extract, mallow extract, senki Sue extract, assembly extract, soybean extract, tiso extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, toshinsen extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, Novara extract, hibiscus extract, bacmond extract, parsley extract, honey, hamamelis extract, parietalia extract, toad extract, bisabolol, loquat extract, dandelion extract, burdock extract, bukuro extract, butcher bloom extract, grape extract, propolis, loofah extract, Safflower extract, peppermint extract, body sage extract, button extract, hop extract, pine extract, maroonnier extract, mizubasho Kiss, Mukuroji extract, Melissa extract, Peach extract, Cornflower extract, Eucalyptus extract, Yukinoshita extract, Yokuinin extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Lotus root extract, Rose extract, Rosemary extract, Roman chamomile extract And royal jelly extract.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Immunostimulants such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester Vitamins, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherols, carotenoids, flavonoids, tannins, lignans, saponins, α-hydroxy acids, β-hydroxy acids, etc. Cell activators, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione, etc. Whitening agent, cephalanthin, licorice extract, red pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pan Tothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, pyroctone Olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantalis tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, monostearic acid Examples include hair growth agents such as polyoxyethylene sorbitan, mint oil, and Sasanishiki extract.

(10) Examples of antioxidants: sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.

(11) Examples of solvents Examples include purified water, ethanol, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, and next-generation fluorocarbon.

〔Example〕
Production of Fucoxanthin-Containing Kelp Extract After 21 parts by weight of raw kelp (macomb) was immersed in water for 48 hours, 50.8 parts by weight of 99 wt% ethanol was added, and extraction was performed at 40 degrees for 1 hour. Thereafter, filtration was performed to separate the residue from 64 parts by weight of the extract (primary extract). The residue was further extracted with 50.8 parts by weight of 99 wt% ethanol at 50 ° C. for 1 hour, and then filtered to separate the residue from 49.0 parts by weight of the extract (secondary extract). .
64 parts by weight of the primary extract and 19 parts by weight of the secondary extract were mixed to prepare a mixed solution. The mixed solution was subjected to column treatment to obtain a fucoxanthin-containing kelp extract. In addition, three types of fucoxanthin-containing kelp extracts (Examples) were obtained in the same manner.

[Test example]
1. Test method 2 x 10 ⁴ cultured brown adipocytes from 3rd generation
Prepared to cells / mL and seeded 500 μL each in a 24-well plate. The next day, the medium was replaced with a differentiation-inducing medium and cultured for 48 hours. Next, the culture medium was replaced with a maintenance medium, cultured for 48 hours, and then replaced with a maintenance medium containing the fucoxanthin-containing kelp extract of the above example. After culturing for 24 hours, the cells were collected and examined for the expression of various UCP1 and UCP2) mRNAs.
The results are shown in FIG. 1 (UCP1) and FIG. 2 (UCP2).

Results and Effects of Examples As shown in FIG. 1, in the expression of UCP-1 mRNA, expression was promoted by treatment with a low concentration of fucoxan. Thereby, it was confirmed that fucoxanthin and the kombu extract containing the same promote the expression of the UCP-1 gene at a low concentration, thereby having an obesity preventing effect.
Further, according to FIG. 2, the expression of UCP-2 mRNA was promoted at a high concentration. Thereby, it was confirmed that it has an obesity preventing effect even at a high concentration.

[Example of formulation]
Although the compounding example of the UCP gene expression promoter (fucoxanthin containing kelp extract) of this invention is given to the following, the following compounding example does not limit this invention.
Formulation Example 1: Chewing gum sugar 53.0 wt%
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Fucoxanthin-containing kelp extract 0.5
100.0wt%

Formulation Example 2: Gummy reduced starch syrup 40.0 wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Grape juice 4.0
Grape flavor 0.6
Dye 0.02
Fucoxanthin-containing kelp extract 1.0
100.0wt%

Formulation Example 3: Candy sugar 50.0 wt%
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Fucoxanthin-containing kelp extract 0.4
100.0wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Fucoxanthin-containing kelp extract 0.4
Perfume
Water residue
100.0wt%

Formulation Example 5: Soft Drink Fructose Glucose Liquid Sugar 30.0wt%
Emulsifier 0.5
Fucoxanthin-containing kelp extract 0.05
Perfume
Purified water residue
100.0wt%

Formulation Example 6: Soft capsule grape seed oil 87.0 wt%
Emulsifier 12.0
Fucoxanthin-containing kelp extract 1.0
100.0wt%

Formulation Example 7: Tablet Lactose 54.0 wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Fucoxanthin-containing kelp extract 1.0
100.0wt%

Formulation Example 8: Oral granules (pharmaceuticals)
Fucoxanthin-containing kelp extract 1.0 wt%
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 8.0
Polyvinylpyrrolidone 1.0
100.0wt%

Formulation Example 9: Tablet confectionery sugar 76.4 wt%
Glucose 19.0
Sucrose fatty acid ester 0.2
Fucoxanthin-containing kelp extract 0.5
Purified water 3.9
100.0wt%

Formulation Example 10: Cosmetic cream squalane 20.0 wt%
Beeswax 5.0
Refined jojoba oil 5.0
Glycerin 5.0
Glycerol monostearate 2.0
Polyoxyethylene (20) sorbitan-
Monostearate 2.0
Fucoxanthin-containing kelp extract 2.0
Antiseptic agent
Purified water residue
100.0wt%

Formulation Example 11: Lotion ethanol 5.0 wt%
Glycerin 2.0
1,3-butylene glycol 2.0
Polyethylene oleyl ether 0.5
Sodium citrate 0.1
Citric acid 0.1
Fucoxanthin-containing kelp extract 0.1
Purified water residue
100.0wt%

Formulation Example 12: Body gel macadamia nut oil 2.0 wt%
Octyldodecyl myristate 10.0
Methylphenylpolysiloxane 5.0
Behenyl alcohol 3.0
Stearic acid 3.0
Batyl alcohol 1.0
Glyceryl monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit 2.0
Hydrogenated soybean phospholipid 1.0
Ceramide 0.1
Retinol palmitate 0.1
Preservative Appropriate amount Clover extract 1.0
Fucoxanthin-containing kelp extract 1.0
1,3-butylene glycol 5.0
Purified water residue
100.0wt%

Formulation Example 13: Latex squalane 4.0 wt%
Vaseline 2.5
Cetanol 2.0
Glycerin 2.0
Lipophilic glyceryl monostearate 1.0
Stearic acid 1.0
L-Arginine 1.0
Fucoxanthin-containing kelp extract 0.5
Potassium hydroxide 0.1
Perfume
Purified water residue
100.0wt%

Formulation Example 14: Bath agent (liquid)
Propylene glycol 50.0wt%
Ethanol 20.0
Sodium sulfate 5.0
Fucoxanthin-containing kelp extract 0.5
Lanolin 0.5
Avocado oil 0.5
Dye 1.5
Fragrance 22.0
100.0wt%

Formulation Example 15: Cat food corn 34.0 wt%
Flour 35.0
Meat meal 15.0
Beef tallow 8.9
Salt 1.0
Skipjack extract 4.0
Fucoxanthin-containing kelp extract 1.0
Taurine 0.1
Vitamins 0.5
Minerals 0.5
100.0wt%

Formulation Example 16: Dog food corn 30.0 wt%
Meat (chicken) 15.0
Defatted soybean 10.0
Flour 25.0
Mosses 5.0
Fucoxanthin-containing kelp extract 5.0
Animal fats and oils 8.9
Oligosaccharide 0.1
Vitamin 0.5
Mineral 0.5
100.0wt%

  As described above, the present invention provides a safe UCP gene expression promoter comprising a novel ingredient as an active ingredient.

Claims (2)

UCP gene expression promoter containing fucoxanthin as an active ingredient. A UCP gene expression promoter comprising as an active ingredient at least one extract selected from algae containing fucoxanthin.