JP2009035538A - Functional food containing laver - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a lipid absorption promoter, a food composition, a functional food or food kit for special uses, containing laver or a laver extract, and also the food promoting the lipid absorption. <P>SOLUTION: This lipid absorption promoter containing the laver, also the lipid absorption promoter in which the laver is powdered, the lipid absorption promoter further containing the lipid are provided. By containing the laver, the absorption of the lipid is promoted. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a lipid absorption promoter, food composition, functional food or special-purpose food kit containing laver or laver extract.

  Japan has a long history of using many seaweeds as food resources and industrial raw materials, and recently, the functionality of seaweeds such as seaweed and kombu has attracted attention. Seaweed contains vitamin B1, vitamin B2, vitamin K, folic acid, dietary fiber, potassium, calcium, iron, magnesium, and minerals. Among them, potassium, magnesium, and alginic acid contained in kombu all have blood pressure. In addition to being said to have a lowering effect, it has also been reported that it has a neutral fat absorption inhibitory effect and an anti-obesity effect (Non-patent Document 1). Furthermore, it has been reported that green algae (Caulerpa taxifolia) has an inhibitory action on pancreatic lipase activity (Patent Document 1).

  In addition, the nutritional effect of seaweed has been attracting attention. Nori contains a lot of dietary fiber, and nearly 40% is dietary fiber. It is known that dietary fiber has the effect of normalizing cholesterol metabolism and preventing blood cholesterol from rising. Furthermore, the main component of the cell walls of the seaweed is a water-soluble dietary fiber called porphyran, which is a galactose sulfate-based polysaccharide, and is known to improve innate immune activity (Patent Document 2). The seaweed is also rich in vitamins such as vitamin A, vitamin B1, vitamin B2, vitamin C, and vitamin B12. Furthermore, a lot of eicosapentaenoic acid (EPA) is contained, and about 50% of the fatty acids of nori are EPA. EPA is said to be effective in preventing thrombosis and in reducing neutral fat. Furthermore, the laver contains taurine at a high concentration of 1 to 2%, and 30 to 36 mg per laver. Taurine lowers cholesterol and is said to be effective for arteriosclerosis, stroke caused by vascular disorders, and myocardial infarction. It also contains abundant minerals such as calcium and iodine, protein, iron (Non-Patent Document 2).

  Furthermore, by adding oils and fats to 40% by weight or more of the total amount, the technology for improving the absorption rate of β-carotene contained in seaweed (Patent Document 3) and grinding the seaweed to a size of 20 μm or less A technique for enhancing innate immune activity and antioxidant action inherent in laver by pulverization is disclosed (Patent Document 4).

  On the other hand, the number of kidney disease patients is increasing year by year, and it is said that 30,000 new dialysis patients are increasing every year. In kidney disease, blood is not filtered well, water and sodium are likely to accumulate in the body, and waste that should be released into the urine increases in the body. As a result, nitrogen increases in the blood and causes uremia, and sodium increases and hypertension occurs (Non-patent Document 3).

  Therefore, diet therapy is very important in kidney disease. Dietary therapy requires maintaining proper salt, water, protein, and energy levels. Protein restriction is particularly important, and if the amount of protein to be consumed increases, the kidneys are considerably burdened.

  Adult men need about 2200 kcal of energy per day, and adult women need about 1800 kcal of energy per day. However, patients with kidney disease cannot get energy from protein due to protein intake restriction, and they tend to be short of energy. is there. Furthermore, the restriction on protein intake makes it difficult to ingest high-calorie foods mainly composed of protein, such as meat, fish, and soybeans, and the accompanying fats and oils cannot be ingested. Become. It has been found that patients with kidney disease who have become deficient in energy worsen their condition by further burdening the kidneys due to the breakdown of proteins in the body. Therefore, it is important to efficiently ingest energy from lipids and carbohydrates, particularly from lipids.

  In addition, patients with malabsorption syndrome and the elderly are obstructed by the absorption of various nutrients taken orally, especially lipids, and may become undernourished depending on the degree and duration of the disorder.

  The malabsorption syndrome includes various illnesses, but it is broadly divided into primary malabsorption syndrome based on abnormalities in nutrient absorption process itself and secondary malabsorption syndrome secondary to the causative illness. The Primary malabsorption syndrome includes sprue and intestinal enzyme deficiency. Secondary malabsorption syndrome is caused by intestinal lesions such as Crohn's disease, amyloidosis, digestive enzymes in pancreatic cancer and biliary tract cancer after intestinal resection. There are various diseases such as secretion disorders (Non-patent Document 4).

  There are methods to improve nutritional status by providing diet and nutrition therapy for patients with malabsorption syndrome and the elderly, but it is important to promote absorption of ingested nutrients and improve bioavailability It is believed that.

https://www.fujicco.co.jp/corp/press/pr_20060818_2.pdf http://www8.ocn.ne.jp/~isekan/newpage25.html http://www.gik.gr.jp/~skj/ht/ht-kidney.php3 http://health.goo.ne.jp/medical/search/10G31800.html Japanese Patent Laid-Open No. 10-203974 JP 2004-339161 A Japanese Patent Laid-Open No. 2002-300865 JP 2003-319757 A

  Kidney disease diets are protein-restricted, so patients with kidney disease need to get more energy from lipids, and if the absorption of lipids consumed by malabsorption syndrome patients or the elderly is reduced It is necessary to absorb ingested lipids efficiently. Currently, supplements containing medium chain fatty acids have been developed, but if there is a material that can efficiently absorb lipids ingested with meals, it is also very useful as a supplement to be taken with meals.

  This invention is made | formed in view of the said situation, and it aims at providing the technique which accelerates | stimulates absorption of the lipid which mammals including humans ingested.

  In the process leading to the present invention, experiments were conducted for the purpose of effective use of seaweed ingredients in foods and pharmaceuticals. The inventor initially searched for substances having anti-obesity action contained in seaweed-derived components because obesity tends to induce various diseases and is closely related to lifestyle-related diseases. Therefore, as shown in the examples below, the present inventor conducted a lipid tolerance test using rats, and contrary to expectation, the blood triglyceride concentration increased in the group administered laver compared to the control group. Results were obtained. Therefore, the present invention was completed by considering that nori is not used as an anti-obesity agent, but can be applied as a lipid absorption promoter from the aspect of promoting lipid absorption.

  That is, according to the present invention, a lipid absorption promoter containing nori is provided. The present invention promotes lipid absorption because laver has a function of promoting lipid absorption, as shown in the Examples below.

  Furthermore, according to this invention, the lipid absorption promoter containing a laver extract is provided. The present invention promotes the absorption of lipids because the laver extract has a function of promoting the absorption of lipids as shown in the examples described later.

  Moreover, according to this invention, the food composition for lipid absorption promotion containing nori is provided. The present invention promotes lipid absorption because laver has a function of promoting lipid absorption, as shown in the Examples below.

  Moreover, according to this invention, the functional food which displays that the absorption of a lipid is promoted including nori is provided. In the present invention, as shown in the examples described later, since nori has a function of promoting the absorption of lipid, the absorption of lipid is promoted.

  In addition, according to the present invention, there is provided a special-purpose food kit containing laver and promoting lipid absorption. Since the present invention includes nori, it promotes absorption of lipids that are ingested as special-purpose foods such as kidney disease foods and nursing foods.

  Moreover, according to this invention, the fat-soluble functional component absorption promoter containing nori is provided. The present invention promotes the absorption of fat-soluble nutritional functional components that coexist with lipids, because laver has the function of promoting the absorption of lipids, as shown in the Examples below.

  Moreover, according to this invention, the food composition for promoting absorption of a fat-soluble nutrient functional component containing laver is provided. The present invention promotes the absorption of fat-soluble nutrient functional components that coexist with lipids because the laver extract has the function of promoting the absorption of lipids, as shown in the Examples described later.

  Moreover, according to this invention, the functional food which displays that the absorption of a fat-soluble nutrient functional component is accelerated | stimulated including nori is provided. The present invention promotes the absorption of fat-soluble nutritional functional components that coexist with lipids, because laver has the function of promoting the absorption of lipids, as shown in the Examples below.

  In addition, according to the present invention, there is provided a special-purpose food kit for promoting absorption of fat-soluble nutritional functional components, including laver. Since the present invention includes nori, it promotes absorption of fat-soluble nutritional functional components that are ingested as special-purpose foods such as kidney disease foods and nursing foods.

  According to the present invention, as shown in Examples described later, the lipid absorption is promoted because it has a function of promoting lipid absorption. Moreover, according to this invention, as shown in the below-mentioned Example, since nori has the function to accelerate | stimulate absorption of a lipid, absorption of the fat-soluble nutrient functional component which coexists with a lipid is accelerated | stimulated.

Embodiments of the present invention will be described below.
<Background>
The inventor initially conducted experiments for the purpose of effectively using seaweed ingredients in foods and pharmaceuticals. In particular, various effects on obesity and lifestyle-related diseases have been studied using experimental animals. Obesity easily induces various diseases and is closely related to lifestyle-related diseases, and therefore, food materials that exhibit anti-obesity action have been widely searched. For example, it has been reported that yew ivy has a pancreatic lipase activity inhibitory action, and that kombu has a neutral fat absorption inhibitory effect and an anti-obesity action. The present inventor also initially searched for a substance having an anti-obesity action contained in seaweed-derived components. Therefore, as shown in the examples described later, the effect of seaweed on lipid absorption was tested using lipid-loaded rats. Contrary to expectation, the blood triglyceride concentration increased in the group to which nori was administered compared to the control group. The result of doing was obtained. Therefore, it was considered that it can be applied as a lipid absorption promoter from the aspect of promoting lipid absorption, not being used as an anti-obesity agent.

<Overview>
This embodiment is a lipid absorption promoter containing laver. As shown in the examples described later, laver promotes lipid absorption because it has a function of promoting lipid absorption.

  Furthermore, this embodiment is a lipid absorption enhancer containing a laver extract. As shown in Examples described later, the laver extract has a function of promoting lipid absorption, and therefore promotes lipid absorption.

  Moreover, this embodiment is a food composition for promoting lipid absorption, comprising nori. As shown in the Examples described later, since the seaweed has a function of promoting lipid absorption, this food composition also promotes lipid absorption.

  In addition, the present embodiment is a functional food that contains nori and displays that lipid absorption is promoted. As shown in the examples described later, since laver has a function of promoting lipid absorption, absorption of lipid is also promoted by this functional food.

  Moreover, this embodiment is a special purpose food kit for promoting lipid absorption, including nori. As shown in the examples below, laver has a function of promoting the absorption of lipids, and therefore has the effect of promoting the absorption of ingested lipids even when used as a special-purpose food such as a kidney disease food or a nursing food. Play.

  Moreover, this embodiment is a fat-soluble functional component absorption promoter containing laver. As shown in the examples described later, because laver has a function of promoting lipid absorption, it promotes absorption of fat-soluble nutrient functional components that coexist with lipid.

  Moreover, this embodiment is a food composition for promoting absorption of fat-soluble nutritional functional components, comprising nori. As shown in the Examples described later, since the laver extract has a function of promoting the absorption of lipids, it promotes the absorption of fat-soluble nutrient functional components that coexist with the lipids.

  Moreover, this embodiment is a functional food containing laver and displaying that absorption of fat-soluble nutritional functional components is promoted. As shown in the examples described later, because laver has a function of promoting lipid absorption, it promotes absorption of fat-soluble nutrient functional components that coexist with lipid.

  Moreover, this embodiment is a special-use food kit for promoting absorption of fat-soluble nutritional functional components, including laver. Because it contains nori, it promotes absorption of fat-soluble nutritional functional ingredients that are ingested as special-purpose foods such as kidney disease foods and nursing foods.

<Nori>
Seaweed is a general term for seaweed that is used for food of red algae, cyanobacteria and green algae. When the seaweed is further categorized, it is a dry and red seaweed called seaweed or rock seaweed (a seaweed of the genus Amanori, which is a primordial red algae such as Asakusanori), and a green seaweed that is used as a raw material for boiled or fried oysters. Seaweeds (green algae Aosa or Aonori seaweeds), kawanori, cyanobacteria suizenjinori.

  In the present invention, the seaweed is particularly dried. The dried seaweed includes dried seaweed, grilled seaweed, seasoned seaweed, rose dried seaweed, and round dried seaweed. Dry seaweed is chopped laver raised from the sea, washed with water and then dried to about 10%, but its manufacturing process includes plucking, foreign matter removal, shredding and washing, blending and papermaking, Dehydration, drying, and peeling are performed in this order. The baked seaweed is obtained by further heating the dried seaweed to reduce the moisture to about 4% and baking it. Rose dried seaweed and round dried seaweed are those obtained by plucking, removing foreign substances, washing, dehydrating and drying in the dry seaweed production method.

  The dried seaweed is black in color and has a unique scent of persimmon. In addition, the grilled seaweed has a stronger flavor and better crispness than the dry seaweed. However, dry seaweed and baked seaweed produced using discolored seaweed may differ in color and scent from normal seaweed dry seaweed and baked seaweed, but as shown in the examples below, lipid absorption promotion There is no change in that it has an effect. Here, discolored seaweed refers to a lighter color than normal seaweed. In addition, it has been reported that the discolored nori has a lower nutrient content compared to normal nori (http://www.affrc.go.jp/seika/data_kinki/h16/11_tikusan/ p435 / index.html).

<Powder>
The powder means a powder pulverized to an average particle size of 100 μm or less, preferably 50 μm or less, more preferably 20 μm or less. In the following examples, pulverization was performed using a stone mill type mill. As a raw material for the powder, dry nori may be used, but preferably grilled nori is used. When grilled laver is used, the cell wall is easily destroyed, which not only has the advantage of increasing the productivity of the crushing process. Absorbability can be improved.

  Generally, since the size of the nori cell is 20-30 μm, the cell walls of some nori cells are destroyed by grinding to 100 μm or less. Moreover, if it grind | pulverizes to 50 micrometers or less, the cell wall of many laver cells will be destroyed. Furthermore, if it grind | pulverizes to 20 micrometers or less, all the cell walls will be destroyed. As a result, various nutrients such as vitamins and EPA contained in the cytoplasm and nucleus of the seaweed are absorbed more quickly and easily in the body, and the immune activation action, intestinal regulation action, blood pressure lowering action, Antioxidant action, cholesterol lowering action, etc. are enhanced.

<Oil and fat>
Oils and fats mean oils and fats in a broad sense and refer to those based on lipids. For example, rapeseed oil (canola oil), salad oil, white squeeze oil, corn oil, soybean oil, sesame oil, rice bran oil, coconut oil, coconut oil, safflower oil (safflower oil), palm oil, palm shell oil, coconut oil, cottonseed oil, Sunflower oil, camellia oil, mustard oil, kapok oil, sea bream oil, castor oil, tung oil, olive oil, peanut oil, almond oil, avocado oil, hazelnut oil, walnut oil, grape seed oil, fish oil, liver oil, salmon oil , Lard, head (beef tallow), chicken oil, Schmalz, shortening, butter, margarine, cacao butter, hydrogenated oil and the like, preferably rapeseed oil. Rapeseed oil contains about 60% of the fatty acid contained in it, oleic acid, the smallest saturated fatty acid among oils and fats, and contains vitamin K that prevents osteoporosis, so that it can be expected not only to promote fat absorption but also to prevent osteoporosis. There is also an advantage that it is relatively difficult to oxidize.

<Lipid>
Lipid is a general term for fatty acids and fatty acid derivatives, and is the main component constituting fats and oils. Lipids are classified into simple lipids (neutral fats, etc.), complex lipids (phospholipids, etc.), and derived lipids (cholesterol, fatty acids, etc.) based on differences in chemical structure. The lipids used herein include structural lipids, which are lipids obtained by artificially modifying fatty acids, and diglycerides that are said to have an effect of reducing body fat accumulation and preventing obesity. Monoglyceride (monoacylglycerol) is one esterified with one of the three hydroxyl groups of glycerol, monoglyceride (monoacylglycerol), diglyceride (diacylglycerol) is esterified with two fatty acids, and three fatty acids are esterified These are called triglycerides (triacylglycerols), and these glycerides are contained in neutral fats.

<Fatty acid>
Fatty acid refers to a chain-like monovalent carboxylic acid and can be obtained by hydrolysis from simple lipids or complex lipids. Fatty acids that do not have double or triple bonds between carbon atoms are called saturated fatty acids (including palmitic acid), and those that have one or more double or triple bonds are unsaturated fatty acids (such as oleic acid). Is included). Further, it has been found that EPA and DHA, which are one of fatty acids, are contained in a large amount in fish oil, and conjugated EPA and DHA have a cell-killing effect on human cultured cancer cells. Conjugated linoleic acid is a fatty acid that is abundant in beef tallow and milk and is said to suppress the occurrence of cancer.

<Mixing with lipid>
Nori may be mixed with lipids. By mixing the laver and the lipid, not only the absorption of the lipid is promoted, but also the absorption of the fat-soluble nutritional functional component contained in the laver can be promoted.

<Mixing ratio>
If lipid absorption is promoted, the mixing ratio of the seaweed may be 10 parts by weight or more and 90 parts by weight or less, preferably 60 parts by weight on the 20 parts by weight when the total weight with the lipid is 100 parts by weight. Or less, more preferably 20 parts by weight or more and 40 parts by weight or less. In particular, in order to maintain a fluid state, the amount is preferably 40 parts by weight or less. In the examples described later, nori powder and fats and oils are mixed in a ratio of 3 to 7 (30 parts by weight when the total weight of the fats and oils is 100 parts by weight).

<Dosage form>
The dosage form of the fat absorption enhancer of this embodiment may be powder, pill, tablet, capsule, liquid for internal use, extract, suppository, or injection. Preferably, the dosage form is a powder, a tablet, a capsule, and more preferably a capsule. Capsules include hard capsules and soft capsules, and are characterized by being easy to carry and easy to drink. Specific examples of the powder include powders, granules, fine granules and the like. Specific examples of tablets include tablets, naked tablets, and sugar-coated tablets.

<Food form>
The form of the food of this embodiment is not particularly limited, for example, confectionery in the shape of a stick or biscuit, mixed with liquid food, sweetened and other drinks, medical food, The thing mixed in special purpose foods, such as nursing food, may be sufficient. Preferred are medical foods and nursing foods.

<Medical meal>
The medical diet of this embodiment is not particularly limited, but is a diet for adjusting the amount of nutrition according to the disease that the patient is suffering to prevent the progression of the disease or to improve the disease, It may also be a medical meal for patients who are required to promote lipid absorption such as patients with malabsorption syndrome. Specific examples of this medical treatment include those obtained by adding seaweed to general medical treatments, and those obtained by adding seaweed to staple foods or side dishes.

<Care meal>
The care food of this embodiment is not particularly limited, but is a meal that has been processed to be easy to eat so that it can cope with people who have decreased eating and swallowing functions. For elderly people who cannot sufficiently absorb nutrients from the ingested food, the diet may be processed so as to be easily ingested and swallowed to promote nutrient absorption. As a specific example of this nursing food, laver is added to a general nursing food, or laver is added to a staple food or a side dish, and the amount of water contained is increased compared to a normal food. And those that have been softly processed, those that have been better consolidated by a laver component, and the like.

<Effect>
Hereinafter, the effect of this embodiment is demonstrated.
In the present embodiment, experiments were conducted for the purpose of effectively using seaweed components for foods and pharmaceuticals. In particular, obesity easily induces various diseases and is closely related to lifestyle-related diseases. Therefore, at first, a substance having an anti-obesity action contained in seaweed-derived components was searched. For example, it has been reported that yew ivy has an inhibitory action on pancreatic lipase activity, and that kombu has a neutral fat absorption inhibitory effect and an anti-obesity action, and conventionally, seaweeds inhibit lipid absorption. It was thought that there were many things. Therefore, as shown in the Examples described later, when a lipid tolerance test was performed using rats, contrary to expectation, a result that blood triglyceride concentration increased in the group administered nori was higher than that in the control group. It was. Therefore, it was considered that it can be applied as a lipid absorption promoter from the aspect of promoting lipid absorption, not being used as an anti-obesity agent. That is, the lipid absorption promoter containing the laver of this embodiment has a function that the laver promotes the absorption of fat, so that the absorption of lipid is promoted as shown in the experimental data of the examples described later. An effect is obtained.

  Furthermore, this embodiment may be a lipid absorption promoter containing powdered nori. In this case, since the seaweed is powdered, various nutrients such as vitamins and EPA contained in the cytoplasm and nucleus of the seaweed are absorbed more quickly and easily in the body, resulting in immune activity. It has the effect of enhancing the action of rectification, intestinal regulation, blood pressure lowering, antioxidant, cholesterol lowering and the like.

  In addition, the present embodiment may be a lipid absorption enhancer further containing a lipid. In this case, since it contains lipids, even when only the lipid absorption enhancer of this embodiment is ingested, it can not only absorb nutrients from lipids but also promote absorption of fat-soluble nutrient functional components contained in laver Play.

  In addition, the present embodiment may be a lipid absorption promoter in which the amount of nori mixed is 20 to 40 parts by weight of the total weight of the fat and oil. In this case, absorption of lipid is promoted as shown in Examples described later.

  In addition, the present embodiment may be a lipid absorption promoter containing rapeseed oil-derived lipid. In this case, rapeseed oil is expected to have not only a fat absorption promoting effect but also an osteoporosis preventing effect since rapeseed oil contains oleic acid in about 60% of the fatty acid, and the fatty acid is the smallest in fatty acids and contains vitamin K that prevents osteoporosis. There is also an advantage that it is difficult to oxidize.

  Further, the present embodiment may be a lipid absorption promoter containing baked seaweed. In this case, roasted laver has the advantage that the cell wall is easily destroyed, so that the productivity of the crushing process is increased, and the nutrients in the laver are easily eluted, and the vitamins contained in the cytoplasm and nucleoplasm of the laver Various nutrients such as EPA and EPA are absorbed more quickly and easily in the body, and the immune activation action, bowel regulation action, blood pressure lowering action, antioxidant action, cholesterol lowering action, etc. are enhanced. Play.

  Moreover, this embodiment may be a lipid absorption promoter containing a laver extract. In this case, there is an advantage that there is a lipid absorption promoting action even when only the laver extract is used instead of the whole laver. The term “nori extract” as used herein refers to an extract of nori components, preferably obtained by hot water extraction, more preferably 60% to 90% of the hot water extract supernatant. This is a precipitate precipitated with ethanol. In the examples described later, it is shown that the 75% ethanol precipitate fraction of the supernatant of the laver hot water extract has a lipid absorption promoting action.

  Further, the present embodiment may be a lipid absorption promoter containing nori and processed into a capsule. In this case, since it is processed into a capsule, there is an advantage that it is easy to carry and easy to drink.

  Further, the present embodiment may be a food composition for promoting lipid absorption, comprising nori. In this case, since the seaweed has a function of promoting fat absorption, lipid absorption is promoted as shown in experimental data of Examples described later.

  In addition, the present embodiment may be a functional food containing laver and displaying that lipid absorption is promoted. In this case, since nori has a function of promoting fat absorption, the absorption of lipid is promoted as shown in the experimental data of Examples described later.

  In addition, the present embodiment is a kidney disease food kit that includes nori and promotes lipid absorption. Since laver has a function of promoting fat absorption, it has an effect of promoting absorption of lipids ingested as a kidney disease food, as shown in experimental data of Examples described later.

  As mentioned above, although embodiment of this invention was described, these are illustrations of this invention and various structures other than the above are also employable.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further, this invention is not limited to these.

Example 1 Effect of nori in lipid-loaded rats Test method (1) Test sample Grilled seaweed and rapeseed oil pulverized with natural powder (Chaya Sangyo Co., Ltd.) are mixed in a ratio of 3 to 7 (when the total weight of oil and fat is 100 parts by weight) 30 parts by weight), heated and pressurized, and sterilized were used as test substances. The lipid emulsion was prepared by ultrasonic treatment of 3 ml of rapeseed oil, 100 mg of bile acid, and 3 ml of pure water.

(2) Experimental animals 10-12 week old Kud: Wistar male rats were purchased from Kudo Co., Ltd. and reared in a light cycle of 23 ° C. and 12 hours. Preliminary breeding was carried out for one week, during which time the solid breeding feed CE-2 (Nippon Claire Co., Ltd.) was ad libitum and used as free drinking water.

(3) Treatment of animals Fasting male and female Kud: Wistar rats aged 11 to 13 weeks overnight and dividing them into a control group and a test group so that there is no significant difference in body weight. In the control group, 1 ml of lipid emulsion and 2 ml / 350 g body weight of pure water were added and orally administered under non-anaesthesia while adjusting so that there was no difference in the lipid intake of each group. Blood was collected from the tail vein under non-anaesthesia at 30, 60, 120, 180, 240, and 300 minutes after administration, with 0 minutes before administration.

(4) Measurement of blood lipid concentration After the collected blood was centrifuged at 3000 rpm, serum was fractionated. Serum triglyceride (TG) concentration was measured using Triglyceride-E-Test Wako (Wako Pure Chemical Industries, Ltd.).

(5) Statistical processing Before performing the significant difference test, we confirmed the equal variance of the data by Bartlett's equal variance test. A significant difference test was performed. Significant difference test was performed by Student's t-test. Moreover, it determined with P <0.01 having a significant difference.

2. Results Effect on blood triglyceride concentration The results are shown in FIG. Sixty minutes after administration, the blood triglyceride concentration in the test group showed a significantly higher value than that in the control group, and then showed a high tendency.

3. Discussion From the results of this test, it was shown that baked laver powder has the effect of promoting the absorption of coexisting lipids. In particular, an increase in blood triglyceride concentration is observed 60 minutes after ingestion, suggesting not only an increase in absorption efficiency but also an effect of increasing the absorption speed. The above results indicate that fat absorption promoters containing baked laver powder are very useful for improving the utilization efficiency of lipids ingested in meals, and improve the pathology of patients with kidney disease and malabsorption syndrome patients. It seems to be useful.

Example 2 Effect of seaweed extract on lipid absorption Test method (1) Sample As a test substance, Tosacori, Chrome and Nori are extracted with hot water, cooled, extracted for another 12 hours, and each supernatant is extracted with Tosakanori hot water extract supernatant (TE), A hot water extract supernatant (KE) and a laver hot water extract supernatant (NE) were used. As for laver, 75% ethanol was added to the supernatant of the obtained hot water extract and divided into a precipitated fraction and a non-precipitated fraction, and the 75% ethanol precipitated fraction ( NP) and 75% ethanol non-precipitated fraction (NS) of hot water extract supernatant of seaweed.

(2) Effect of seaweed extract on pancreatic lipase activity Uniform sonication of 9 mg of triolein 80 mg, lecithin 10 mg, and bile acid 5 mg in 9 ml of 0.1 M Tris buffer (pH 7.0) for 10 minutes A suspension was obtained, and this suspension was used as a substrate solution. As an experimental operation, 0.05 ml of pancreatic lipase solution (final concentration 1 μg / ml) and 0.1 ml of a test substance were added to 0.1 ml of a substrate solution, reacted at 37 ° C. for 30 minutes, and free fatty acid was quantified by a copper reagent method. did. The absorbance when the test substance was not added was taken as 100%, and the effect when the test substance was added was measured. The final concentration of the test substance to be added was 2 or 4 mg / ml, respectively.

(3) Effect of added amount of nori extract on pancreatic lipase activity Nori hot water extract (NE), 75% ethanol precipitate fraction (NP) of nori hot water extract supernatant, and nori hot water extraction Regarding the 75% ethanol non-precipitated fraction (NS) of the product supernatant, the influence of the amount of laver added on the pancreatic lipase activity was examined. Ultrasonic treatment of 80 mg of triolein, 10 mg of lecithin and 5 mg of bile acid in 9 ml of 0.1 M Tris buffer (pH 7.0) for 10 minutes makes a uniform suspension. This suspension is used as a substrate solution. As an experimental procedure, 0.05 ml of pancreatic lipase solution (final concentration 1 μg / ml) and 0.2, 1, 2 or 4 mg / ml of nori extract were added to 0.1 ml of the substrate solution, and reacted at 37 ° C. for 30 minutes. The free fatty acid was quantified by the copper reagent method. The effect of the amount added was measured with the absorbance when no seaweed was not added as 100%.

(4) Effects of seaweed extract on lipid-loaded rats (i) Experimental animals 16-22 week old Kud: Wistar male rats were purchased from Kudo Co., Ltd. and reared in a light cycle at room temperature 23 ° C for 12 hours. did. Preliminary breeding was carried out for one week, during which time the solid breeding feed CE-2 (Nippon Claire Co., Ltd.) was ad libitum and used as free drinking water.

(Ii) Treatment of animals 17-23 week old Kud: Wistar male rats are fasted overnight and divided into a test group and a control group so that there is no significant difference in body weight. In the experiment for examining the effect of the seaweed-derived component on the pancreatic lipase activity of), a 75% ethanol precipitate fraction (NP) of the hot water extract supernatant of seaweed that significantly inhibited the lipase activity was added to the lipid emulsion. In the control group, 1 ml of lipid emulsion and 4 ml / 350 g body weight of pure water were added and orally administered under non-anaesthesia while adjusting so that there was no difference in the lipid intake of each group. Blood was collected from the tail vein under non-anaesthesia at 30, 60, 120, and 180 minutes after administration, with 0 minutes before administration.

(Iii) Measurement of blood lipid concentration After the collected blood was centrifuged at 3000 rpm, serum was fractionated. Serum triglyceride concentration was measured using Triglyceride-E-Test Wako (Wako Pure Chemical Industries, Ltd.).

(5) Statistical processing Before performing the significant difference test, we confirmed the equal variance of the data by Bartlett's equal variance test. A significant difference test was performed. Significant difference test was performed by Student's t-test. Moreover, it determined with P <0.001 having a significant difference.

2. Results As a result of comparing the pancreatic lipase activity of the seaweed extract, the laver was the strongest and suppressed the pancreatic lipase activity compared to Tokasori and Kurome (FIG. 2). Among them, the 75% ethanol precipitation fraction of the seaweed hot water extract supernatant strongly suppressed pancreatic lipase activity (FIG. 3). Next, when the effect of lipids on intestinal absorption of this fraction was examined, the blood triglyceride concentration after lipid administration was significantly higher than that of the control group after 30 minutes (FIG. 4). Moreover, the effect of the seaweed shown in the present Example was similarly observed even when the discolored seaweed was used.

3. Discussion In general, when lipase activity is suppressed, it is considered that lipid is prevented from being broken down into monoglycerides, fatty acids or glycerol in the digestive tract, and lipid absorption in small intestinal epithelial cells is reduced. In the present study, in a test examining the effect of seaweed extract on pancreatic lipase activity, the result that laver extract suppresses lipase activity was obtained. From the fact that yew ivy, which is a green alga, also has a lipase activity inhibitory action (Patent Document 1) and that the kombu has an anti-obesity action (Non-Patent Document 1), the lipase activity inhibitory action is an action found in all seaweeds. It is predicted.

  However, in a study examining the effects of seaweed extract in lipid-loaded rats, the 75% ethanol precipitate fraction of the laver hot water extract supernatant has an action that promotes an increase in blood triglyceride concentration, that is, lipid absorption. It was found to have a promoting effect. This result is considered to be an effect that cannot be predicted by past literature and those skilled in the art.

  In addition, as shown in the present example, the 75% ethanol precipitate fraction of the seaweed hot water extract supernatant had a lipid absorption promoting action as in Example 1, so that the lipid absorption promoting action was This suggests the possibility of the action of components that can be extracted by the above extraction method.

  In general, the fraction purified by hot water extraction and 75% ethanol precipitation is thought to contain polysaccharides and proteins. It can be said that the polysaccharide has an effect as a surfactant in that it is adsorbed at the interface and reduces the interfacial energy. Therefore, it is possible that the action of polysaccharides as a surfactant contributed to the micellization of lipids in the gastrointestinal tract, and promoted lipid absorption in small intestinal epithelial cells. is necessary.

  From this test result, it was shown that the 75% ethanol-precipitated fraction of the seaweed hot water extract supernatant is very useful for promoting absorption of lipids ingested in the diet. In general, it has been reported that the nutritional components of discolored seaweed are rich in moisture and Ca and less in crude protein, coarse fat, coarse fiber and phosphorus compared to dry seaweed. Even in the case, it was shown that the lipid absorption promoting action can be obtained. From the above, it is considered that the laver hot water extract is useful for promoting the absorption of lipids in patients with kidney disease, patients with malabsorption syndrome and the elderly.

Example 3 Effect of laver in lipid-loaded vitamin-loaded rats Test method (1) Test sample Grilled seaweed and rapeseed oil pulverized with natural powder (Chaya Sangyo Co., Ltd.) are mixed in a ratio of 3 to 7 (when the total weight of oil and fat is 100 parts by weight) 30 parts by weight), heated and pressurized, and sterilized were used as test substances. Moreover, the lipid emulsion was prepared by adding vitamin E to 3 ml of rapeseed oil, 100 mg of bile acid, and 3 ml of pure water and subjecting it to ultrasonic treatment.

(2) Experimental animals 8 week old Kud: Wistar male rats were purchased from Kudo Co., Ltd. and reared in a light cycle at room temperature of 23 ° C. for 12 hours. Preliminary breeding was carried out for one week, during which time the solid breeding feed CE-2 (Nippon Claire Co., Ltd.) was ad libitum and used as free drinking water.

(3) Treatment of animals 9-week-old Kud: Wistar male rats are fasted overnight and divided into a control group and a test group so that there is no significant difference in body weight. A group to which 1 ml of lipid emulsion and 2 ml / 350 g body weight of pure water were added was orally administered to each group under non-anaesthesia while adjusting so that there was no difference in each group's lipid intake. Blood was collected from the tail vein under non-anaesthesia 1 hour after administration, with 0 minutes before administration.

(4) Measurement of blood vitamin E concentration The collected blood was centrifuged at 3000 rpm, and the serum was fractionated. The vitamin E concentration in serum was extracted by the method of Burton et al. And measured using high performance liquid chromatography.

(5) Statistical processing The significant difference test was performed by Welch's t-test. Moreover, it was determined that there was a significant difference when p <0.05.

2. Results The results are shown in the figure. The value was expressed as a transport amount by subtracting the value before administration from the value 60 minutes after administration. As a result, the blood vitamin E concentration in the test group was significantly higher than that in the control group (FIG. 5).

3. Discussion From the results of this test, it was shown that the burned laver powder has an action of promoting the absorption of coexisting vitamin E. This test result shows that absorption of other fat-soluble nutritional functional components is also promoted by ingesting together with laver powder.

  In general, fat-soluble nutrient functional components such as vitamin A, vitamin E, vitamin K, and ubiquinone are deeply involved in maintaining human health and preventing lifestyle-related diseases. Although bioavailability is an important factor for the functional expression of such nutritional functional ingredients, the digestibility and absorption of these fat-soluble nutritional functional ingredients are significantly lower than other nutritional ingredients and the bioavailability is low. It has been known.

  Digestion and absorption of fat-soluble nutritional functional components are the process of digestion until release from food, dispersion in the digestive tract, solubilization in mixed micelles, and uptake into small intestinal epithelial cells and transfer to lymph fluid It is a complex process consisting of In the above process, it is known that absorption of fat-soluble nutritional functional components is enhanced by ingesting lipids. According to the above examples, it was shown that seaweed assists the digestion and absorption of fat-soluble nutrient functional components by increasing the digestion and absorption rate of lipids, so it can be used as a food that assists in the absorption of fat-soluble nutrient functional components it can.

  In particular, fat-soluble nutritional functional components such as vitamin E contained in seaweed are not only efficiently absorbed, but by adding fat-soluble nutritional functional components to seaweed, absorption of desired fat-soluble nutritional functional components can be promoted. The possibility was shown.

  Here, the fat-soluble nutritional functional ingredient is fat-soluble and has a physiological activity that affects the maintenance of animal functions, and includes, for example, fat-soluble vitamins, fat-soluble antioxidant pigments, and the like. . The fat-soluble vitamin means a fat-soluble vitamin in a broad sense, and is a generic name for vitamins that are difficult to dissolve in water and easily soluble in oil (fat), including vitamin A (including provitamin A such as β-carotene), vitamin D, Vitamin E, vitamin K, vitamin Q (coenzyme Q10), vitamin F (essential fatty acid), vitamin P (quercetin, hesperidin, rutin) and the like are included. The fat-soluble antioxidant pigment means a fat-soluble antioxidant pigment in a broad sense, which means that it has an antioxidant action that is hardly soluble in water and easily soluble in oil (fat). For example, carotenes (α-carotene, β-carotene, lycopene, etc.), xanthophylls (lutein, zeaxanthin, etc.), polyphenols (flavonoids such as anthocyanins and catechins, curcumin, etc.) and the like. In addition, as fat-soluble nutritional functional components not belonging to fat-soluble vitamins or fat-soluble antioxidant pigments, α-lipoic acid, oryzanols, lignans (such as sesamin) and the like are also included in the fat-soluble nutritional functional components.

  In the above, this invention was demonstrated based on the Example. It is to be understood by those skilled in the art that this embodiment is merely an example, and that various modifications are possible and that such modifications are within the scope of the present invention.

FIG. 3 shows rat serum triglyceride concentration in Example 1. FIG. When there was a significant difference between each group, it was displayed using *. The value of the pancreatic lipase activity of the seaweed component in Example 2 is shown. The value of the pancreatic lipase activity of nori in Example 2 is shown. NE shows the value of group pancreatic lipase activity to which a hot water extract of seaweed was added. NP shows the value of the group pancreatic lipase activity to which 75% ethanol precipitation fraction of the hot water extract supernatant of seaweed was added. NS shows the value of pancreatic lipase activity of the group to which 75% ethanol non-precipitated fraction of the hot water extract supernatant of seaweed was added. FIG. 3 shows rat serum triglyceride concentration in Example 2. FIG. The control indicates the blood triglyceride concentration of the control group. NP indicates the blood triglyceride concentration of the group administered with the 75% ethanol precipitation fraction of the hot water extract supernatant of seaweed. When there was a significant difference between each group, it was displayed using *. The vitamin-E density | concentration in rat serum in Example 3 is shown. The control indicates the blood vitamin E concentration of the control group. The test substance group shows the blood vitamin E concentration of the group to which the burned laver powder was administered. * Indicates that there is a significant difference between the groups.

Claims (16)

  Lipid absorption promoter containing nori.   The lipid absorption promoter according to claim 1, wherein the laver is powdered.   The lipid absorption enhancer according to claim 1, further comprising a lipid.   The lipid absorption promoter according to claim 3, wherein when the total weight of the seaweed and the lipid is 100 parts by weight, the weight of the seaweed is mixed at a ratio of 20 parts by weight or more and 40 parts by weight or less.   The lipid absorption enhancer according to claim 3, wherein the lipid is derived from rapeseed oil.   The lipid absorption promoter according to claim 1, wherein the laver is grilled laver.   The lipid absorption enhancer according to claim 1, which is processed into a capsule.   The lipid absorption promoter according to claim 1, further comprising a fat-soluble nutritional functional ingredient.   Lipid absorption promoter containing nori extract.   A food composition for promoting lipid absorption, comprising nori.   A functional food containing nori and displaying that lipid absorption is promoted.   Special purpose food kit for promoting lipid absorption, including nori.   Fat-soluble functional ingredient absorption promoter containing laver.   A food composition for promoting absorption of fat-soluble nutritional functional ingredients, comprising nori.   A functional food containing nori and indicating that absorption of fat-soluble nutritional functional ingredients is promoted.   Special-purpose food kit for promoting absorption of fat-soluble nutritional functional ingredients, including nori.

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