JP2008516637A - 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み - Google Patents

真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Download PDF

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Publication number
JP2008516637A
JP2008516637A JP2007538145A JP2007538145A JP2008516637A JP 2008516637 A JP2008516637 A JP 2008516637A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2008516637 A JP2008516637 A JP 2008516637A
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Prior art keywords
trna
amino acid
protein
unnatural amino
amino acids
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Abandoned
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JP2007538145A
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Japanese (ja)
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JP2008516637A5 (enExample
Inventor
ピーター・ジー．・シュルツ
サラ・アール．・ハンソン
ラン・スー
ツィーウェン・ツァング
カイ−ヒューイ・ウォン
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Scripps Research Institute
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Scripps Research Institute
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Publication of JP2008516637A publication Critical patent/JP2008516637A/ja
Publication of JP2008516637A5 publication Critical patent/JP2008516637A5/ja
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
JP2007538145A 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Abandoned JP2008516637A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62089804P 2004-10-20 2004-10-20
PCT/US2005/038250 WO2006045116A2 (en) 2004-10-20 2005-10-20 In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria

Publications (2)

Publication Number Publication Date
JP2008516637A true JP2008516637A (ja) 2008-05-22
JP2008516637A5 JP2008516637A5 (enExample) 2008-12-11

Family

ID=36203749

Family Applications (1)

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JP2007538145A Abandoned JP2008516637A (ja) 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み

Country Status (4)

Country Link
US (3) US20080064059A1 (enExample)
EP (1) EP1809758A4 (enExample)
JP (1) JP2008516637A (enExample)
WO (1) WO2006045116A2 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
US20050287639A1 (en) 2004-05-17 2005-12-29 California Institute Of Technology Methods of incorporating amino acid analogs into proteins
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2624290A1 (en) * 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
ATE509027T1 (de) * 2006-01-13 2011-05-15 Pasteur Institut Enzymatische synthese von grossen mengen von mucin glyconjugaten und deren immunogenische anwendungen
JP5808882B2 (ja) 2006-03-03 2015-11-10 カリフォルニア インスティテュート オブ テクノロジー 分子へのアミノ酸の部位特異的な組み込み
CA2638763A1 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現
BRPI0711811A2 (pt) * 2006-05-23 2011-12-06 Scripps Research Inst aminoácidos de cumarina fluorescente geneticamente codificados
CA2663052A1 (en) * 2006-09-21 2008-03-27 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
US20080166766A1 (en) 2006-10-18 2008-07-10 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
GB0716719D0 (en) * 2007-08-29 2007-10-10 Chromatide Ltd Process for carrying out a chemical reaction
KR20100088618A (ko) * 2007-10-25 2010-08-09 더 스크립스 리서치 인스티튜트 리덕타제 내로의 3-아미노티로신의 유전적 도입
US20090148887A1 (en) * 2007-11-02 2009-06-11 The Scripps Research Institute Genetically encoded boronate amino acid
BRPI0819092A2 (pt) 2007-11-02 2014-10-14 Scripps Research Inst Evolução direcionada usando proteínas que compreendem aminoácidos não naturais.
CN105647824A (zh) * 2007-12-11 2016-06-08 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
KR20100125290A (ko) * 2008-02-08 2010-11-30 더 스크립스 리서치 인스티튜트 유전적으로 코딩된 비천연 아미노산에 의한 면역 내성의 파괴
US8637306B2 (en) * 2008-12-10 2014-01-28 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US9051593B2 (en) 2009-12-21 2015-06-09 Trustees Of Dartmouth College Recombinant prokaryotes and use thereof for production of O-glycosylated proteins
WO2015100277A2 (en) * 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
US12359192B2 (en) * 2013-12-23 2025-07-15 University Of Rochester Methods and compositions for display of macrocyclic peptides
WO2017085718A1 (en) * 2015-11-17 2017-05-26 Anima Biotech Inc. FUNCTIONAL TRANSFER tRNA-APTAMER MOLECULES
WO2019074833A1 (en) * 2017-10-09 2019-04-18 Indiana University Research And Technology Corporation SUGAR-RELATED AMINO ACIDS FOR THE SYNTHESIS OF SOLID-STATE PEPTIDES
WO2021041507A1 (en) * 2019-08-26 2021-03-04 The Johns Hopkins University Methods for identifying o-linked glycosylation sites in proteins
JP2024501288A (ja) * 2020-12-23 2024-01-11 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー 修飾ｔｒｅｍの組成物及びその使用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0481038B1 (en) 1990-04-16 2002-10-02 The Trustees Of The University Of Pennsylvania Saccharide compositions, methods and apparatus for their synthesis
US5369017A (en) * 1994-02-04 1994-11-29 The Scripps Research Institute Process for solid phase glycopeptide synthesis
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
ES2561985T3 (es) 2001-10-10 2016-03-01 Ratiopharm Gmbh Remodelación y glicoconjugación de anticuerpos
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
JP5319863B2 (ja) 2002-12-26 2013-10-16 独立行政法人理化学研究所 体細胞相同組換えの誘発方法
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof

Also Published As

Publication number Publication date
US20080064059A1 (en) 2008-03-13
EP1809758A4 (en) 2009-04-01
WO2006045116A2 (en) 2006-04-27
US7378263B2 (en) 2008-05-27
US20060110796A1 (en) 2006-05-25
EP1809758A2 (en) 2007-07-25
US20080241903A1 (en) 2008-10-02
WO2006045116A3 (en) 2006-12-28

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