JP2008516637A - 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み - Google Patents

真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Download PDF

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Publication number
JP2008516637A
JP2008516637A JP2007538145A JP2007538145A JP2008516637A JP 2008516637 A JP2008516637 A JP 2008516637A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2007538145 A JP2007538145 A JP 2007538145A JP 2008516637 A JP2008516637 A JP 2008516637A
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Prior art keywords
trna
amino acid
protein
unnatural amino
amino acids
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Abandoned
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JP2007538145A
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Japanese (ja)
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JP2008516637A5 (enExample
Inventor
ピーター・ジー．・シュルツ
サラ・アール．・ハンソン
ラン・スー
ツィーウェン・ツァング
カイ−ヒューイ・ウォン
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Scripps Research Institute
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Scripps Research Institute
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Publication of JP2008516637A publication Critical patent/JP2008516637A/ja
Publication of JP2008516637A5 publication Critical patent/JP2008516637A5/ja
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
JP2007538145A 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み Abandoned JP2008516637A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62089804P 2004-10-20 2004-10-20
PCT/US2005/038250 WO2006045116A2 (en) 2004-10-20 2005-10-20 In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria

Publications (2)

Publication Number Publication Date
JP2008516637A true JP2008516637A (ja) 2008-05-22
JP2008516637A5 JP2008516637A5 (enExample) 2008-12-11

Family

ID=36203749

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007538145A Abandoned JP2008516637A (ja) 2004-10-20 2005-10-20 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み

Country Status (4)

Country Link
US (3) US7378263B2 (enExample)
EP (1) EP1809758A4 (enExample)
JP (1) JP2008516637A (enExample)
WO (1) WO2006045116A2 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL158418A0 (en) * 2001-04-19 2004-05-12 Scripps Research Inst In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
EP1666604B1 (en) * 2003-07-07 2008-02-13 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
US20050287639A1 (en) 2004-05-17 2005-12-29 California Institute Of Technology Methods of incorporating amino acid analogs into proteins
EP2383340B1 (en) * 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) * 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
EP1808442B1 (en) * 2006-01-13 2011-05-11 Institut Pasteur Enzymatic large-scale synthesis of mucin glyconjugates, and immunogenic applications thereof
EP1999259B1 (en) 2006-03-03 2014-06-25 California Institute of Technology Site-specific incorporation of amino acids into molecules
WO2007103490A2 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
BRPI0709603A2 (pt) * 2006-03-16 2011-07-19 Scripps Research Inst expressão de proteìnas geneticamente programada contendo o aminoácido não natural fenilselenocisteìna
BRPI0711811A2 (pt) * 2006-05-23 2011-12-06 Scripps Research Inst aminoácidos de cumarina fluorescente geneticamente codificados
RU2009110152A (ru) * 2006-09-21 2010-10-27 Зе Скрипс Ресеч Инститьют (Us) Генетически закодированная формула селективно-сульфатных белков в эубактерии
KR20090094812A (ko) 2006-10-18 2009-09-08 더 스크립스 리서치 인스티튜트 포유동물 세포에서 비천연 아미노산의 단백질 내로의 유전적 도입
GB0716719D0 (en) * 2007-08-29 2007-10-10 Chromatide Ltd Process for carrying out a chemical reaction
EP2217707B1 (en) 2007-10-25 2014-10-15 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
EP2220218A4 (en) * 2007-11-02 2010-12-08 Scripps Research Inst GENETICALLY ENCODED BORONATE AMINO ACID
US9249408B2 (en) * 2007-11-02 2016-02-02 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
CA2708760A1 (en) * 2007-12-11 2009-06-18 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
BRPI0906388A2 (pt) * 2008-02-08 2015-07-07 Scripps Research Inst Quebra de tolerância imunológica com um aminoácido não natural geneticamente codificado
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US9051593B2 (en) 2009-12-21 2015-06-09 Trustees Of Dartmouth College Recombinant prokaryotes and use thereof for production of O-glycosylated proteins
US12359192B2 (en) * 2013-12-23 2025-07-15 University Of Rochester Methods and compositions for display of macrocyclic peptides
EP3087088B1 (en) * 2013-12-23 2022-05-25 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
WO2017085718A1 (en) * 2015-11-17 2017-05-26 Anima Biotech Inc. FUNCTIONAL TRANSFER tRNA-APTAMER MOLECULES
EP3694525A4 (en) * 2017-10-09 2021-07-07 Indiana University Research & Technology Corporation SUGAR-BOUND AMINO ACIDS FOR THE SYNTHESIS OF PEPTIDES IN THE SOLID PHASE
US20220299522A1 (en) * 2019-08-26 2022-09-22 The Johns Hopkins University Compositions and methods for identifying o-linked glycosylation sites in proteins
EP4107258A4 (en) * 2020-02-20 2024-04-17 B.G. Negev Technologies and Applications Ltd., at Ben-Gurion University MUTANT AMINOACYL-ARNT SYNTHETASES
BR112023012377A2 (pt) * 2020-12-23 2023-10-24 Flagship Pioneering Innovations Vi Llc Composições de trems modificadas e usos das mesmas

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016449A1 (en) * 1990-04-16 1991-10-31 The Trustees Of The University Of Pennsylvania Saccharide compositions, methods and apparatus for their synthesis
US5369017A (en) * 1994-02-04 1994-11-29 The Scripps Research Institute Process for solid phase glycopeptide synthesis
IL158418A0 (en) 2001-04-19 2004-05-12 Scripps Research Inst In vivo incorporation of unnatural amino acids
EP2042196B1 (en) 2001-10-10 2016-07-13 ratiopharm GmbH Remodelling and glycoconjugation of Granulocyte Colony Stimulating Factor (G-CSF)
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004035743A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins
JP5319863B2 (ja) 2002-12-26 2013-10-16 独立行政法人理化学研究所 体細胞相同組換えの誘発方法
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
EP1666604B1 (en) 2003-07-07 2008-02-13 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Also Published As

Publication number Publication date
WO2006045116A2 (en) 2006-04-27
EP1809758A4 (en) 2009-04-01
EP1809758A2 (en) 2007-07-25
WO2006045116A3 (en) 2006-12-28
US20080241903A1 (en) 2008-10-02
US20060110796A1 (en) 2006-05-25
US20080064059A1 (en) 2008-03-13
US7378263B2 (en) 2008-05-27

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