JP2008505071A - 細胞を遺伝学的に活性化する方法、および、該細胞の使用 - Google Patents
細胞を遺伝学的に活性化する方法、および、該細胞の使用 Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/41—Vertebrate antigens
- A61K40/42—Cancer antigens
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2510/00—Genetically modified cells
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- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
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| US58485904P | 2004-07-02 | 2004-07-02 | |
| PCT/SE2005/001049 WO2006004518A1 (en) | 2004-07-02 | 2005-06-30 | Method for the genetic activation of cells and uses of said cells |
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| JP2008505071A true JP2008505071A (ja) | 2008-02-21 |
| JP2008505071A5 JP2008505071A5 (cg-RX-API-DMAC7.html) | 2008-08-14 |
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| EP (1) | EP1781777A1 (cg-RX-API-DMAC7.html) |
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| WO (1) | WO2006004518A1 (cg-RX-API-DMAC7.html) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016539105A (ja) * | 2013-11-01 | 2016-12-15 | ナントクエスト インコーポレイテッド | 殺腫瘍性および抗菌性の組成物ならびに方法 |
| KR20170131569A (ko) * | 2015-03-27 | 2017-11-29 | 난트케이웨스트, 인크. | 암의 치료를 위한 유전적으로 변형된 nk-92 세포 및 모노클로날 항체 |
| JP2018517415A (ja) * | 2015-06-10 | 2018-07-05 | ナントクエスト インコーポレイテッド | がんを処置するための改変ｎｋ−９２細胞 |
| JP2018522592A (ja) * | 2015-07-29 | 2018-08-16 | オーエヌケーイミューン リミテッド | 細胞傷害性が増加した改変ナチュラルキラー細胞及びナチュラルキラー細胞株 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120220549A1 (en) | 2009-09-03 | 2012-08-30 | Michael Stephen Starch | Pituitous Silicone Fluids |
| KR102727338B1 (ko) | 2018-10-31 | 2024-11-06 | 난트퀘스트, 인크. | Pd-l1 키메라 항원 수용체-발현 nk 세포에 의한 pd-l1-양성 악성종양의 제거 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998049268A1 (en) * | 1997-04-30 | 1998-11-05 | Hans Klingemann | Natural killer cell lines and methods of use |
| WO2003044183A1 (en) * | 2001-11-22 | 2003-05-30 | Medigenes | Interleukin-2 gene transferred lymphokine activated killer cells |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ295673A (en) * | 1994-11-01 | 1999-03-29 | Wels Wilfried | Nucleic acid transfer system using recombinant multi-domain proteins each domain having a different function |
| US6034072A (en) * | 1997-02-10 | 2000-03-07 | Genemedicine, Inc. | IL-2 gene expression and delivery systems and uses |
| GB0116318D0 (en) * | 2001-07-03 | 2001-08-29 | 4 The Environment Ltd | Processing of waste materials |
| GB0318096D0 (en) * | 2003-08-01 | 2003-09-03 | Queen Mary & Westfield College | Vaccine |
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2005
- 2005-06-30 EP EP05754800A patent/EP1781777A1/en not_active Withdrawn
- 2005-06-30 JP JP2007519168A patent/JP2008505071A/ja active Pending
- 2005-06-30 WO PCT/SE2005/001049 patent/WO2006004518A1/en not_active Ceased
- 2005-07-01 US US11/171,397 patent/US20060029583A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998049268A1 (en) * | 1997-04-30 | 1998-11-05 | Hans Klingemann | Natural killer cell lines and methods of use |
| WO2003044183A1 (en) * | 2001-11-22 | 2003-05-30 | Medigenes | Interleukin-2 gene transferred lymphokine activated killer cells |
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| US11304977B2 (en) | 2013-11-01 | 2022-04-19 | ImmunityBio. Inc. | Tumoricidal and antimicrobial compositions and methods |
| JP2016539105A (ja) * | 2013-11-01 | 2016-12-15 | ナントクエスト インコーポレイテッド | 殺腫瘍性および抗菌性の組成物ならびに方法 |
| US10258649B2 (en) | 2013-11-01 | 2019-04-16 | Nantkwest, Inc. | Tumoricidal and antimicrobial compositions and methods |
| US10517895B2 (en) | 2013-11-01 | 2019-12-31 | Nantkwest, Inc. | Tumoricidal and antimicrobial compositions and methods |
| US10646516B2 (en) | 2013-11-01 | 2020-05-12 | Nantkwest, Inc. | Tumoricidal and antimicrobial compositions and methods |
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2006004518A1 (en) | 2006-01-12 |
| US20060029583A1 (en) | 2006-02-09 |
| EP1781777A1 (en) | 2007-05-09 |
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