JP2008289805A - Biosensor measuring instrument - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor measuring instrument which integrally includes a blood sampling auxiliary mechanism using depressurization, thereby raise monitoring simplification and measurement success probability simultaneously. <P>SOLUTION: The biosensor measuring instrument includes: an airtight chamber 47 which is arranged inside a blood collection auxiliary mechanism storage body 45 and communicates with a puncture opening 81 of the blood collection auxiliary mechanism storage body 45, and where a mounting chamber for mounting a biosensor cartridge 43 is airtightly closed by a measuring instrument body; a pressure block 49 freely movably arranged in the airtight chamber 47 and pressurizing the biosensor cartridge 43 with energizing force from energizing means 89; a lever 51 freely slidably appearing to the outer part of the blood sampling auxiliary mechanism storage body 45 and displacing the energizing means 89 to an energizing force storage direction; a depressurization pump 53 arranged in communication with the airtight chamber 47, moving a piston 69 by the slide of the lever 51, and depressurizing the airtight chamber 47; and a puncture button for releasably restricting the movement of the lever 51 which is moved to the energizing force storage direction. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a biosensor measurement device, and more particularly to a biosensor measurement device that measures and analyzes chemical substances using a reagent contained in a hollow reaction part of a loaded biosensor cartridge.

  Conventionally, a biosensor in which a biosensor chip and a lancet are integrated has been disclosed (see, for example, Patent Document 1). As shown in FIG. 8, the lancet-integrated sensor 11 includes a chip body 13, a lancet 15, and a protective cover 17. The chip body 13 has a cover 19 and a substrate 21 that can be opened and closed. An internal space 23 is formed on the inner surface of the cover 19. The internal space 23 accommodates the lancet 15 so as to be movable.

  A puncture device (needle) 25 provided at the tip of the lancet 15 can be projected and retracted from an opening 27 formed at the front end of the internal space 23 of the tip body 13 as the lancet 15 moves. . The shape of the internal space 23 is curved so that the width of the inner space 23 is slightly narrower than that of the lancet 15 at the end where the protrusion 29 is located, and the lancet 15 is locked to the chip body 13 by mutual pressing force and frictional force. It is like that. The protective cover 17 has a tube portion 31 into which the needle 25 is inserted, and the tube portion 31 can be accommodated inside the chip body 13 as the needle 25 moves. In a state before use, the protective cover 17 is covered with the needle 25 to protect it, so that the user is not accidentally injured. The substrate 21 is provided with a pair of electrode terminals 33, which can be electrically connected to measuring means of a measuring device (not shown).

The lancet-integrated sensor 11 configured as described above removes the protective cover 17 and pushes the lancet 15 to protrude the needle 25 from the tip body 13 when in use. After puncturing the subject in this state, the needle 25 is housed inside the chip body 13, and the opening 27 provided at the front end is brought close to the puncture port of the subject to collect the outflowed blood.
International Publication No. 02/056769 Pamphlet

The above-described conventional lancet-integrated sensor enables blood components to be monitored by electrically connecting electrode terminals to a measuring device. For example, since a blood glucose measurement device used for early detection and prevention of deterioration of diabetes is used for measurement many times a day, it is preferable that the lancet integrated sensor and the measurement device are integrated. Moreover, in monitoring, in order to improve a measurement success probability, it is desirable to provide a decompression type blood collection assist mechanism. The decompression-type blood collection assisting mechanism has an airtight chamber and houses a puncture mechanism therein. The hermetic chamber is connected to a decompression pump, and sucks blood that has flowed out from the puncture port of the subject perforated by the puncture device in the hermetic chamber by the negative pressure obtained by the decompression pump.
However, in the airtight chamber, when a spring that presses the puncture device is installed, the cocking lever that contracts this is exposed to the outside of the device, so these members must be connected to each other, ensuring airtightness. However, it is difficult to provide an airtight chamber and a vacuum pump integrally. Further, since the lancet-integrated sensor loaded in the hermetic chamber must be electrically connected to the measuring means, an electric wire is connected to the movable sensor pressing means, and the electric wire is not degraded. It must be led out from the hermetic chamber, and it has become more difficult to realize a measuring device in which a lancet-integrated sensor, a measuring device, and a reduced-pressure blood collection assist mechanism are integrated.
The present invention has been made in view of the above situation, and provides a biosensor measurement device that can be integrally provided with a blood collection assist mechanism by decompression, thereby simultaneously improving the convenience of monitoring and the probability of success of measurement. Objective.

The above object of the present invention is achieved by the following configuration.
(1) A biosensor measurement device that loads a biosensor cartridge having a puncture device at the tip, punctures the subject with the puncture device of the biosensor cartridge, and measures a biological material in the sample Because
The biosensor measuring device has a measuring device main body and a blood collection assisting mechanism housing,
An airtight chamber that is provided in the blood collection assisting mechanism housing and communicates with a puncture opening of the blood collection assisting mechanism housing and in which a loading chamber for loading the biosensor cartridge can be hermetically sealed by the measurement device body;
A pressing block that is movably provided in the hermetic chamber and presses the biosensor cartridge with a biasing force from a biasing means;
A lever that is slidably exposed to the outside of the blood collection assisting mechanism housing and displaces the biasing means in the biasing force accumulation direction;
A decompression pump provided in communication with the hermetic chamber and moving a piston by sliding the lever to apply a negative pressure to the hermetic chamber;
A puncture button for releasably restricting movement of the lever moved in the biasing force accumulation direction;
Comprising
Furthermore, a measuring means for measuring a sample is provided in the blood collection assisting mechanism housing,
The pressing block is provided with a connection terminal that conducts to the biosensor chip of the biosensor cartridge,
The conduction means for connecting the measurement means and the connection terminal penetrates the wall of the hermetic chamber and is hermetically sealed with a resin material filled between the conduction means and the through hole. Biosensor measurement device.

According to this biosensor measurement device, when the lever is operated, the urging means is in the urging force accumulation state, and movement is restricted by the puncture button. When the puncture button is pressed in this state, the movement restriction of the lever is unlocked and the lever is slid. By sliding the lever, the vacuum pump is driven, the airtight chamber becomes negative pressure, the pressing block in the airtight chamber is moved, and the puncture device provided at the tip of the biosensor cartridge protrudes from the puncture opening. . As a result, blood flowing out from the puncture port of the subject is efficiently sucked into the biosensor cartridge by the negative pressure in the airtight chamber.
Further, a conduction means for connecting the connection terminal provided in the pressing block and the measurement means is inserted into a through hole formed in the wall of the hermetic chamber, and a resin material is used between the conduction means and the through hole. Since it is hermetically sealed, the detection signal from the biosensor cartridge can be measured by the measuring means without reducing the hermeticity of the hermetic chamber.

(2) The lever that is moved from the biasing force accumulation state in the biasing force release direction and moves to the end position is regulated to be releasable by the puncture button at an intermediate position,
The negative pressure is applied by pressing the puncture button in the biasing force accumulation state,
The puncture device is projected by pressing the puncture button at the midway position,
The biosensor measurement device according to (1), wherein the negative pressure is released by pressing the puncture button at the end position.

  According to this biosensor measurement device, the lever is temporarily stopped at the midway position in the process of moving the lever from the biasing force accumulation state to the end position. At this time, the pressure reducing pump is actuated by sliding the lever from the urging force accumulation state to the midway position, and negative pressure is preliminarily applied to the hermetic chamber. The negative pressure applied to the hermetic chamber acts on the subject pressed against the puncture opening, and the subject surrounded by the puncture opening rises (congests) in the suction state. In this state, when the puncture button is pressed again, the movement restriction of the lever is released again, and the lever is slid from the midway position to the end position. The slide of the lever from the midway position to the end position causes the puncture device to protrude, and further negative pressure is applied to puncture the subject that is raised and is in a state suitable for puncture. . In addition, by applying a negative pressure in advance before puncturing and placing the subject in a raised state, it is possible to create a feeling of adsorption, disperse the concentration of pain at the time of puncturing, and relieve tingling. After the lever slides to the end position, when the puncture button is further pressed, the negative pressure in the hermetic chamber is released.

  According to the biosensor measurement device of the present invention, an airtight chamber in which a biosensor cartridge is loaded, a pressing block that is movably provided in the airtight chamber, a lever that displaces the urging means in the urging force accumulation direction, and the airtight chamber Provided with a decompression pump that applies negative pressure to the lever and a puncture button that restricts movement of the lever so that the lever can be released, so that when the lever is operated, the biasing force is accumulated, and when the puncture button is pressed, the lever The pressure-reducing pump that is slid and is driven by the lever slide causes the hermetic chamber to become negative pressure, and the pressing block in the hermetic chamber is moved so that the puncture device provided at the tip of the biosensor cartridge protrudes from the puncture opening. The Therefore, airtightness is ensured, the negative pressure of the decompression pump can be applied, and blood flowing out from the puncture port of the subject is efficiently aspirated by the negative pressure in the airtight chamber. As a result, a configuration integrally including a blood collection assisting mechanism by decompression can be realized, and simplification of monitoring and improvement in measurement success probability can be achieved at the same time.

DESCRIPTION OF EXEMPLARY EMBODIMENTS Hereinafter, preferred embodiments of a biosensor measurement device according to the invention will be described with reference to the drawings. The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is defined by the terms of the claims, rather than the meanings described above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.
FIG. 1 is a plan view in which a part of the biosensor measuring apparatus according to the present invention is cut out, FIG. 2 is a longitudinal sectional view of the biosensor measuring apparatus shown in FIG. 1, and FIG. FIG. 4 is an enlarged view of the negative pressure release valve shown in FIG. 4B, FIG. 4 is an enlarged perspective view of the puncture device and the connection terminal shown in FIG. 1, and FIG. 4B is a sectional view of the airtight chamber through-hole. FIG.

  The biosensor measurement device 100 according to the present embodiment is loaded with a biosensor cartridge 43 having a puncture device 41 at the tip, and a sample (for example, a sample obtained by puncturing a subject not shown in the drawings) Blood) can be measured. This biosensor measurement device 100 can be suitably used as, for example, the blood glucose measurement device described above. The puncture device 41 includes a needle, a lancet needle, a cannula and the like.

  The biosensor measuring device 100 includes a blood collection assisting mechanism housing body 45, an airtight chamber 47, a pressing block 49, a cocking lever (lever) 51, decompression pumps 53 and 53, measuring means 55, a puncture button 57, The measuring device main body 85 is provided as a main constituent member. Slit holes 61, 61 are formed on both side surfaces of the blood collection assisting mechanism housing 45, and the slit holes 61, 61 penetrate the base shaft 59 of the lever 51 slidably. A knob plate 63 of the lever 51 is fixed to both ends of the base shaft 59, and the knob plate 63 is slid along the slit holes 61 and 61 so that the base shaft 59 can be moved rightward in FIG.

  The piston shafts 65 and 65 of the decompression pumps 53 and 53 and the pressing block 49 of the airtight chamber 47 are fixed to the base shaft 59. The piston shafts 65 and 65 and the pressing block 49 are integrated with the base shaft 59 in the left-right direction of FIG. Moved to. The piston shafts 65 and 65 are hermetically sealed with the through holes of the decompression pumps 53 and 53 by O-rings 67. Pistons 69, 69 are fixed to the tip ends of the piston shafts 65, 65, and the pistons 69, 69 can decompress the decompression chambers 71, 71 in the decompression pumps 53, 53. Each decompression chamber 71, 71 communicates with (in communication with) the internal space of the hermetic chamber 47 through a connecting pipe 73.

  The decompression pumps 53 and 53 are provided with an intake / exhaust chamber on the opposite side of the decompression chambers 71 and 71 with the pistons 69 and 69 interposed therebetween, and the intake and exhaust chambers absorb and exhaust air from the exhaust ports 75 and 75. 69, 69 can be moved smoothly. The decompression pumps 53, 53 can apply a negative pressure to the hermetic chamber 47 by moving the pistons 69, 69 by sliding the lever 51. In addition, coil springs 72 and 72 serving as piston urging means are provided between the pistons 69 and 69 and the inner walls of the decompression chambers 71 and 71.

  The pressing block 49 is formed in a rod shape, and is hermetically sealed with the through hole of the hermetic chamber 47 by an O-ring 77. The hermetic chamber 47 is formed with a loading chamber 79 composed of a pair of spaced apart walls, and the loading chamber 79 loads the biosensor cartridge 43 inward. A puncture opening 81 is continuously provided in the loading chamber 79, and the puncture opening 81 opens the front of the loading chamber 79 inward of the cylindrical protrusion. That is, in the biosensor cartridge 43 loaded in the loading chamber 79, the puncture device 41 projects from the puncture opening 81.

  A loading opening 83 is formed in the upper portion of the loading chamber 79, and the loading opening 83 enables loading of the biosensor cartridge 43 into the loading chamber 79. A measuring device main body 85 also serving as a lid member is attached to the loading opening 83 so as to be openable and closable. The measuring device main body 85 is in close contact with a packing or the like (not shown) provided around the loading opening 83 so that the loading opening 83 is hermetically sealed. It can be closed to the state. The measuring device main body 85 accommodates a control unit (not shown) inside, and the measuring means 55 is controlled by an operation signal generated from the control unit.

  A flange 87 is formed at the central portion of the pressing block 49 in the hermetic chamber 47, and the flange 87 is in contact with one end of a coil spring 89 that is an urging means that is externally attached to the pressing block 49. The other end of the coil spring 89 is in contact with the inner wall of the hermetic chamber 47. In the state shown in FIG. 1, the coil spring 89 is compressed. Therefore, when the lever 51 is slid to the right, the coil spring 89 is further compressed by the flange 87 and enters the urging force accumulation state.

  Two preload notches 91 and a puncture notch 93 are formed in the movement direction on the upper surface of the pressing block 49 led out from the hermetic chamber 47, and the preload notch 91 is arranged closer to the airtight chamber 47 than the puncture notch 93. It is installed.

  A restriction swing arm 95 is provided above the preload notch 91 and the puncture notch 93. The restriction swing arm 95 is supported by the blood collection assisting mechanism housing 45 around a central horizontal shaft 97, and both ends thereof are vertically oriented. Can swing freely. A locking claw 99 is vertically suspended from the tip of the regulating swing arm 95, and the locking claw 99 is locked to either the preload notch 91 or the puncture notch 93 so that the movement of the pressing block 49 can be restricted. .

  A pressing spring 101 is brought into contact with the tip of the regulation swing arm 95 from above, and the pressing spring 101 applies a biasing force in the locking direction of the locking claw 99. That is, the clockwise moment of FIG. The lower end of the puncture button 57 disposed above is brought into contact with the base end of the regulation swing arm 95, and the puncture button 57 can be moved in the vertical direction.

  When the pressing block 49 is moved to the urging force accumulation state (the right end in FIGS. 1 and 2), the restricting swing arm 95 is engaged with the preload notch 91 and restricts the movement of the pressing block 49 by the coil spring 89. . When the puncture button 57 is pressed, the locking claw 99 is detached from the preload notch 91, and the pressing block 49 can be moved by the coil spring 89.

  Then, since the locking claw 99 is urged downward by the pressing spring 101, the regulating swing arm 95 is again latched to the puncture notch 93 that is reached next, and the movement of the pushing block 49 is regulated. To do. In this state, when the puncture button 57 is pressed again, the locking claw 99 comes off from the puncture notch 93, and the pressing block 49 reaches the final movement position after the biosensor cartridge 43 protrudes and stops. The biosensor cartridge 43 is punctured by once protruding the puncture device 41 from the puncture opening 81, and then slightly retracted by an urging means (not shown), whereby the puncture device 41 is removed from the subject. The

  As shown in FIG. 3A, a pressure reducing arm 57 a is connected to the puncture button 57, and the pressure reducing arm 57 a penetrates an intake opening 47 a drilled in the upper wall of the airtight chamber 47 in the vertical direction. An O-ring 48 is fitted on the tip of the decompression arm 57a, and the O-ring 48 is in sliding contact with the inner periphery of the intake opening 47a. The O-ring 48 remains in contact with the inner periphery of the intake opening 47a when the pressing block 49 has reached the end position. When the puncture button 57 is further pressed from this state, as shown in FIG. 3B, the O-ring 48 is released into the pressing block 49, and the outside air flows into the airtight chamber 47 through the intake opening 47a. That is, the negative pressure is released when the puncture button 57 is finally pressed.

Here, the biosensor cartridge 43 will be described.
The biosensor cartridge 43 includes a puncture device 41 and a biosensor chip. In the biosensor chip, the chip body has two substrates facing each other and a spacer layer sandwiched between the substrates. A detection electrode is provided on the surface of at least one of the two substrates on the side of the spacer layer, and the tip is bent in a L-shape in a direction facing each other to maintain a predetermined interval. Yes. A hollow reaction part is formed by two substrates and a spacer layer from the tip part of the chip body to the part where the two detection electrodes face each other. A sample collection port for introducing blood as a sample collected by puncture is provided at the tip of the hollow reaction part. In the hollow reaction part, the detection electrode is exposed, and a reagent that generates an electric current by reacting with glucose in blood by immobilizing an enzyme and a mediator, for example, is provided immediately above or in the vicinity of the detection electrode in the hollow reaction part. It has been. That is, the hollow reaction part is a part where a sample such as blood collected from the sample collection port undergoes a biochemical reaction with the reagent.

  As the material for the substrate and the spacer layer, an insulating material film is selected, and as the insulating material, ceramics, glass, paper, biodegradable material (for example, polylactic acid microorganism-producing polyester), polyvinyl chloride, polypropylene Examples thereof include thermoplastic materials such as polystyrene, polycarbonate, acrylic resin, polybutylene terephthalate and polyethylene terephthalate (PET), thermosetting resins such as epoxy resins, and plastic materials such as UV curable resins. A plastic material such as polyethylene terephthalate is preferable because of its mechanical strength, flexibility, and ease of chip fabrication and processing. Typical PET resins include Melinex and Tetron (trade names, manufactured by Teijin DuPont Films, Inc.), Lumirror (trade names, manufactured by Toray Industries, Inc.), and the like.

  Examples of the reagent include glucose oxidase (GOD). In consideration of the blood sampling burden of the specimen, the volume of the hollow reaction part is preferably 1 μL (microliter) or less, particularly preferably 300 nL (nanoliter) or less. With such a minute hollow reaction part, it is possible to collect a sufficient blood volume of the specimen even if the diameter of the puncture device 41 is small. The diameter of the puncture device 41 is preferably 1000 μm or less.

  It is preferable to provide an elastic body 103 covering the periphery of the puncture device 41 at the tip of the chip body. The elastic body 103 can be formed in, for example, a cylindrical shape having a through hole for forming a sealed space in the center. Since the puncture device 41 is inserted, the through hole is made larger than the outer diameter of the puncture device 41. Moreover, it is preferable that the thickness of the elastic body 103 is such that the tip of the puncture device 41 protrudes, for example, by 0.1 mm. The material of the elastic body 103 is not particularly limited as long as it has elasticity, but rubber or sponge, polyethylene, and polypropylene made of a single polymer or a copolymerized polymer such as silicone, urethane, acrylic, ethylene, and styrene. Polyolefin such as polyethylene, polyester such as polyethylene terephthalate and polybutylene terephthalate, polytetrafluoroethylene, and fluororesin such as PFA which is a copolymer of perfluoroalkoxyethylene and polyfluoroethylene can be used. The rubber elastic body may be solid or hollow.

  The shape of the tip portion of the chip main body corresponds to the shape of the adhesive surface of the elastic body 103, and it is preferable that the tip portion has a planar shape. For example, when the cross-sectional shape of the chip body is flat, a widened portion (not shown) may be provided on the tip surface of the chip body so that the front surface of the elastic body 103 is bonded. In the biosensor chip configured as described above, the shape of the contact surface of the elastic body 103 provided at the tip of the chip body with the tip of the chip corresponds to the shape of the tip of the chip body. The elastic body 103 can be securely attached, and can be prevented from being detached or displaced when a pressing force is applied to the elastic body 103.

  Further, it is desirable that the distal end surface of the elastic body 103 that is in contact with the subject is coated with an adhesive or made of a material such as adhesive silicone or silicone gel. As a result, the adhesion between the elastic body 103 and the subject can be improved, the skidding on the surface of the elastic body 103 in contact with the subject can be suppressed, and the sample collection port and the puncture port of the subject can be prevented from shifting. Moreover, it is desirable that the inner peripheral surface of the through hole is made of a hydrophilic material, or at least the inner peripheral surface is subjected to a hydrophilic treatment. In addition, the elastic body 103 of the biosensor cartridge 43 is formed in a size that allows smooth movement in consideration of being loaded into the loading chamber 79 and protruding.

  A measuring means 55 is provided in the blood collection assisting mechanism housing 45, and the measuring means 55 measures a biological material in a sample collected from a detection signal obtained by the biosensor cartridge 43. Connection terminals 105a and 105b shown in FIG. 4 are provided at the tip 49a of the pressing block 49. The connection terminals 105a and 105b are detection electrodes 107a and 107b of the biosensor chip 107 protruding from the rear end of the biosensor cartridge 43. Contact with. In the blood collection assisting mechanism housing 45, an electric wire 109 serving as a conduction means for connecting the measuring means 55 and the connection terminals 105a and 105b passes through the wall 111 of the hermetic chamber 47. As shown in FIG. 4B, the electric wire 109 and the through hole 113 are hermetically sealed with a filled resin material 115. Note that the electric wire 109 may be inserted through a pipe 117 having an elastic body 118 externally inserted through the through hole 113 and hermetically sealed with a resin material 115 inside as shown in the figure. The pipe 117 is provided with an elastic body 118 to improve the airtightness between the pipe 117 and the through hole 113. However, the pipe 117 can be fitted into the through hole 113 in a press-fit state to obtain the airtightness. In this case, the elastic body 118 can be omitted. Further, the resin material 115 filled in the pipe 117 may not be filled in the entire area of the through hole 113 but may be filled only in the entrance / exit of the through hole 113 to obtain airtightness.

  Thus, in the biosensor measuring apparatus 100, the detection electrodes 107a and 107b of the biosensor cartridge 43 come into contact with the connection terminals 105a and 105b provided on the pressing block 49, and one end of the pressing block 49 is connected to one end. An electric wire 109 that connects and connects the other end to the measuring means 55 is inserted into a through hole 113 formed in the wall 111 of the hermetic chamber 47, and a resin material 115 filled between the electric wire 109 and the through hole 113 is inserted into the resin material 115. And hermetically sealed. As a result, the electric wire 109 is connected to the sensor pressing block 49 movably provided in the hermetic chamber 47 without reducing the hermeticity of the hermetic chamber 47, and the detection signal from the biosensor cartridge 43 is measured. Measurement at 55 is possible.

Next, the operation of the biosensor measurement apparatus 100 will be described.
5 is a longitudinal sectional view before loading the biosensor cartridge, FIG. 6 is a longitudinal sectional view after loading the biosensor cartridge, and FIG. 7 is a longitudinal sectional view when the first puncture button is pressed.
In the biosensor measuring device 100, when the lever 51 is operated in the right direction in FIG. 5 in advance in preparation for puncturing, the coil spring 89 is in a state of accumulating urging force, and the locking claw 99 of the regulating swing arm 95 is notched for the preload 91. The movement of the pressing block 49 is restricted.

  In this state, the pistons 69 and 69 are compressed by the coil springs 72 and 72 and moved to the positions indicated by broken lines in FIG. 1 (see the position of the piston 69A), and the volumes of the decompression chambers 71 and 71 are reduced. Here, as shown in FIG. 5, the measuring device main body 85 that also serves as a lid member is opened, and after loading the biosensor cartridge 43 into the loading chamber 79 from the loading opening 83, the measuring device main body 85 is mounted as shown in FIG. The airtight chamber 47 is closed in a closed state.

  Then, the puncture opening 81 is pressed against the subject. When the puncture button 57 is pressed in this state, as shown in FIG. 7, the pressing block 49 is restricted from moving by the locking claw 99 being locked to the puncture notch 93. That is, the lever 51 is temporarily stopped at the midway position in the process of moving from the biasing force accumulation state to the end position. At this time, the decompression chambers 71 and 71 are operated by the slide of the lever 51 from the biasing force accumulation state to the midway position, and a negative pressure is applied to the airtight chamber 47 in advance.

  The negative pressure applied to the hermetic chamber 47 acts on the subject pressed against the puncture opening 81, and the subject surrounded by the puncture opening 81 rises (congestes) to the suction state. When the puncture button 57 is pressed again in this state, the movement restriction of the lever 51 is released again, and the lever 51 is slid from the midway position to the end position.

  The slide of the lever 51 from the midway position to the end position causes the puncture instrument 41 to protrude, and as the urging force of the coil springs 72, 72 is released, the pistons 69, 69 move and the decompression chamber 71, As the volume of 71 increases, negative pressure is further applied, and puncture is performed on the subject that has been raised and is in a state suitable for puncture. As a result, blood can be allowed to flow out from the puncture port of the subject at the moment of puncture, and the blood that has flowed out is efficiently sucked into the hollow reaction part of the biosensor cartridge 43 by the negative pressure in the airtight chamber 47. In addition, by applying a negative pressure in advance before puncturing and placing the subject in a raised (congested) state, it is possible to cause a large amount of bleeding immediately after the puncture. Furthermore, it is possible to relieve the puncture pain caused by puncture by creating a feeling of adsorption and paralyzing around the puncture opening of the subject. Note that the biosensor measurement device may be configured to reduce the pressure after puncturing so that blood flows out from the puncture port of the subject.

  When blood is introduced into the biosensor cartridge 43, counting starts on a display screen (not shown). Once counting is started, the puncture button 57 is further pressed. When the puncture button 57 is finally pressed, as shown in FIG. 3B, the O-ring 48 is released into the pressing block 49, the negative pressure is released, and the puncture opening 81 may be separated from the subject. It becomes.

  Thereafter, when the blood flowing into the hollow reaction part reacts with the reagent, an electric current is generated, and this electric current passes through the detection electrodes 107a and 107b of the biosensor chip 107, the connection terminals 105a and 105b of the pressing block 49, and the electric wire 109. It is input to the measuring means 55 and the components in the blood are measured.

  Therefore, according to the biosensor measurement apparatus 100 configured as described above, the airtight chamber 47 in which the biosensor cartridge 43 provided in the blood collection assisting mechanism housing 45 is loaded, and the airtight chamber 47 are movably provided. A pressing block 49, a lever 51 for displacing the coil spring 89 in the urging force accumulation direction, decompression pumps 53 and 53 for applying a negative pressure to the hermetic chamber 47, and a puncture button 57 for restricting the movement of the lever 51 so as to be releasable. Therefore, when the coil spring 89 is in the accumulated state of urging force by the operation of the lever 51 and the puncture button 57 is pressed, the lever 51 is slid, and the pressure-reducing pumps 53 and 53 that follow the sliding of the lever 51 cause the airtight chamber 47 to be opened. At the same time, the pressure block 49 in the hermetic chamber 47 is moved and is provided at the tip of the biosensor cartridge 43. Puncture instrument 41 is projected from the puncture opening 81. Therefore, airtightness is ensured, the negative pressure of the decompression pumps 53 and 53 can act, and blood flowing out from the puncture port of the subject is efficiently aspirated by the negative pressure of the airtight chamber 47. As a result, a configuration integrally including a blood collection assisting mechanism by decompression can be realized, and simplification of monitoring and improvement in measurement success probability can be achieved at the same time.

  In the above-described embodiment, the negative pressure is applied to the hermetic chamber 47 as the coil spring 89 moves from the biasing force accumulation state to the end position. However, the biosensor measurement device according to the present invention is configured as follows. The negative pressure can be stored in the decompression pump in accordance with the lever operation when moving the coil spring 89 to the biasing force accumulation state. In this case, an open / close gate is provided between the decompression pump and the hermetic chamber, and a mechanism is provided that opens the open / close gate when the first puncture button 57 is pressed. A mechanism for canceling the movement of the decompression pump piston when the lever 51 is moved by the bias of the coil spring 89 may be provided.

It is the top view which notched a part of biosensor measuring device concerning the present invention. It is a longitudinal cross-sectional view of the biosensor measuring apparatus shown in FIG. It is an enlarged view of a negative pressure release valve in which (a) before release and (b) after release are shown. FIG. 2 is an enlarged explanatory view of a main part, showing an enlarged perspective view of the biosensor cartridge and the connection terminal shown in FIG. It is a longitudinal cross-sectional view of the biosensor measuring apparatus before loading a biosensor cartridge. It is a longitudinal cross-sectional view of the biosensor measuring apparatus after loading a biosensor cartridge. It is a longitudinal cross-sectional view when the first puncture button is pressed. It is structure explanatory drawing which represented the external view of the conventional lancet integrated sensor to (a), and represented the exploded perspective view to (b).

Explanation of symbols

DESCRIPTION OF SYMBOLS 41 Puncture instrument 43 Biosensor cartridge 45 Blood collection assistance mechanism accommodating body 47 Airtight chamber 49 Press block 51 Lever 53 Depressurization pump 55 Measuring means 57 Puncture button 69, 69 Piston 79 Loading chamber 81 Puncture opening 83 Loading opening 85 Measuring device main body 89 Coil spring (Biasing means)
100 Biosensor measuring device 105a, 105b Connection terminal 107 Biosensor chip 109 Electric wire (conduction means)
111 Wall of airtight chamber 113 Through hole 115 Resin material

Claims (2)

A biosensor measuring apparatus for loading a biosensor cartridge having a puncture device at the tip, collecting a sample by puncturing the subject with the puncture device of the biosensor cartridge, and measuring a biological material in the sample ,
The biosensor measuring device has a measuring device main body and a blood collection assisting mechanism housing,
An airtight chamber that is provided in the blood collection assisting mechanism housing and communicates with a puncture opening of the voting assisting mechanism housing and in which a loading chamber for loading the biosensor cartridge can be hermetically sealed by the measuring device main body;
A pressing block that is movably provided in the hermetic chamber and presses the biosensor cartridge with a biasing force from a biasing means;
A lever that is slidably exposed to the outside of the blood collection assisting mechanism housing and displaces the biasing means in the biasing force accumulation direction;
A decompression pump provided in communication with the hermetic chamber and moving a piston by sliding the lever to apply a negative pressure to the hermetic chamber;
A puncture button for releasably restricting movement of the lever moved in the biasing force accumulation direction;
Comprising
Furthermore, a measuring means for measuring a sample is provided in the blood collection assisting mechanism housing,
The pressing block is provided with a connection terminal that conducts to the biosensor chip of the biosensor cartridge,
The conduction means for connecting the measurement means and the connection terminal penetrates the wall of the hermetic chamber and is hermetically sealed with a resin material filled between the conduction means and the through hole. Biosensor measurement device. The lever that is moved in the biasing force release direction from the biasing force accumulation state and moves to the end position is restricted to be releasable by the puncture button at an intermediate position,
The negative pressure is applied by pressing the puncture button in the biasing force accumulation state,
The puncture device is projected by pressing the puncture button at the midway position,
The biosensor measurement device according to claim 1, wherein the negative pressure is released by pressing the puncture button at the end position.

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