JP2008274488A - Biological indigo dyeing method - Google Patents

Biological indigo dyeing method Download PDF

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JP2008274488A
JP2008274488A JP2007120087A JP2007120087A JP2008274488A JP 2008274488 A JP2008274488 A JP 2008274488A JP 2007120087 A JP2007120087 A JP 2007120087A JP 2007120087 A JP2007120087 A JP 2007120087A JP 2008274488 A JP2008274488 A JP 2008274488A
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indigo
solution
composition
dyeing
synthetic
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Yutaka Tokiwa
豊 常盤
Kenji Nakajima
健二 中島
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National Institute of Advanced Industrial Science and Technology AIST
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National Institute of Advanced Industrial Science and Technology AIST
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Abstract

<P>PROBLEM TO BE SOLVED: To provide an indigo dyeing method having a reduced smell characteristic of a fermentation vat liquid to be used in indigo dyeing. <P>SOLUTION: The simple indigo dyeing method having a reduced smell characteristic of a fermentation vat liquid useful in conventional indigo dyeing is achieved by bringing a composition having an indigo reduction ability such as (1) a fermentation vat liquid using "Sukumo" (indigo dye) derived from knotweed, (2) a fermentation vat liquid using "Doroai" (mud indigo) derived from Ryukyu indigo, "Doroai" prepared from Indian indigo etc., a synthetic indigo and an alkali buffer solution for pH 10 adjustment into contact with a yeast essence solution (or suspension), retaining the solution for several days and carrying out indigo dyeing using the solution. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、合成インジゴを用いた簡便な藍染めに関する。より詳細には、酵母エキスなどの溶液あるいは懸濁液に、伝統的な藍染に使用される発酵建液等インジゴ還元能を有する組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させ数日間保持後、この溶液を使用した簡便な藍染法に関する。   The present invention relates to a simple indigo dyeing using synthetic indigo. More specifically, a solution or suspension of yeast extract or the like is added to and brought into contact with a composition having indigo reduction ability, such as fermentation building fluid used in traditional indigo dyeing, synthetic indigo, and an alkaline buffer for pH 10. After holding for several days, it relates to a simple indigo dyeing method using this solution.

現在、インジゴによる染色は、(i) 天然藍、すなわち(i-1)北海道から鹿児島にかけて行われている植物の蓼藍（たであい）の葉を発酵させることによって調製した「すくも」に小麦ふすまや灰汁を入れた液（発酵建液）、あるいは(i-2)沖縄で行われている琉球藍やインド藍を発酵して得られた泥藍に小麦ふすまや灰汁を入れた液（発酵建液）を発酵させる方法（正藍建）、(ii) (i)の発酵建液に化学合成された合成インジゴを添加する方法（割建）、(iii) 蓼藍から調製された「すくも」や泥藍あるいは合成インジゴをハイドロサルファイトで還元する化学建（ハイドロ建）による方法によって調製した染液に布を浸漬することによって行なわれている。   Currently, indigo dyeing uses (i) natural indigo, (i-1) wheat bran on “sukumo”, which is prepared by fermenting leaves of plants from Hokkaido to Kagoshima. (I-2) Liquid containing wheat bran or lye in mud indigo obtained by fermenting Ryukyu indigo or Indian indigo in Okinawa (fermented building) (Ii) (i) the method of adding synthetic indigo chemically synthesized to the fermentation building fluid of (i) (wariken), (iii) "sukumo" prepared from sardine It is carried out by immersing the cloth in a dyeing solution prepared by a chemical construction method (hydro-building) that reduces hydrous sulphide or synthetic indigo with hydrosulfite.

このうち、(i)の発酵建による方法や、(ii)(i)の発酵建液に合成インジゴを加えた方法により染色した製品は、関与する微生物の作用により、より優美にして鮮明な色調が得られ、また、(iii)の化学建による方法に比較して耐水性が強い、変色・退色がないなど多くの利点を有している。しかしながら、これらの方法は管理が非常に難しく相当の経験がないと建てることができないこと、高アルカリ性でかつ有機物を発酵原料として用いるため特有の臭いが発生するこという欠点がある。   Of these, products dyed by the method of (i) fermented building and (ii) (i) added synthetic indigo to the fermented building fluid are more elegant and vivid colors due to the action of the microorganisms involved. In addition, it has many advantages such as strong water resistance and no discoloration or fading compared to the method of (iii) using chemical construction. However, these methods are disadvantageous in that they are very difficult to manage and cannot be built without considerable experience, and that they are highly alkaline and have a characteristic odor because organic substances are used as fermentation raw materials.

現在一般的に行なわれている発酵建法は、天然染料として「すくも」や「泥藍」からインジゴを可溶化した藍の発酵建液又はこの発酵建液に合成インジゴを添加したものに、繊維を漬けることにより染色を行なっているが、この方法では発酵建液を染色可能な状態にするには維持・管理の面でかなり手間がかかるし、また高いアルカリ性が必要なので発酵建液は特有の臭いが発生するこという欠点がある。
そこで、本発明では、手間がかからず、また従来の藍染めに使われる発酵建液特有の臭いを軽減が期待できる藍染法を開発した。 Fermentation construction methods currently in common use are indigo fermented solubilized indigo from “sukumo” and “mud indigo” as natural dyes, or synthetic indigo added to this fermented construction fluid. However, in this method, it takes a lot of work in terms of maintenance and management to make the fermented liquid ready to be dyed, and it requires high alkalinity. There is a disadvantage that odor is generated.
Therefore, in the present invention, an indigo dyeing method has been developed that is less time-consuming and can be expected to reduce the odor peculiar to fermentation building fluids used in conventional indigo dyeing.

本発明者らは、上記目的を達成すべく鋭意検討を加え、酵母エキスなどに、天然の蓼藍から調製された「すくも」の発酵建液、琉球藍やインド藍から調製された「泥藍」の発酵建液等インジゴ還元能を有する組成物、合成インジゴ及びpH 10調整用アルカリ緩衝液を添加・接触させた後、数日間保持した反応液を用いた藍染法を開発し、本発明を完成するに到った。
本発明者らは、酵母エキス、小麦ふすまなどの溶液あるいは懸濁液に、茨城県桜川市真壁町（真壁藍工房、蓼藍由来の「すくも」）、沖縄県名護市（やまあい工房、藍染茶房 藍風；琉球藍由来の「泥藍」）、沖縄県石垣島（島藍、インド藍由来の「泥藍」）から調製された発酵建液等のインジゴ還元能を有する組成物、合成インジゴ及びpH 10調整用アルカリ緩衝液を添加・接触させた後、当該溶液に数日間保持するだけで優れた藍染めが可能になることを見出した。また使用する物質によっては従来から使用されている発酵建液に比べ特有の臭気（不快臭）が少ないものも見出された。 The inventors of the present invention have made extensive studies to achieve the above object, and in addition to the yeast extract and the like, the “Sukumo” fermented building fluid prepared from natural vineyards, the “mud indigo” prepared from Ryukyu indigo and Indian indigo. `` Development of indigo dyeing method using a reaction solution kept for several days after adding and contacting indigo reducing composition such as fermented building fluid, synthetic indigo and alkaline buffer solution for pH 10 adjustment. It came to completion.
The present inventors applied a solution or suspension of yeast extract, wheat bran or the like to Makabe-cho, Sakuragawa City, Ibaraki Prefecture (Makabe Ai Kobo, “Sukumo” derived from Arai), Nago City, Okinawa Prefecture (Yamaai Kobo, Aizome). Indigo-reducing ability composition such as fermented building fluid prepared from Chabo Indigo (Ryukyu Indigo derived “Mud Ai”), Ishigaki Island, Okinawa (Island Ai, Indian Indigo derived “Mud Ai”), synthetic indigo And after adding and contacting the alkaline buffer for pH 10 adjustment, it was found that excellent indigo dyeing can be achieved simply by holding the solution for several days. In addition, depending on the substance used, some were found to have less specific odor (unpleasant odor) than the conventionally used fermentation building fluid.

本発明の要旨は次のとおりである。
すなわち、本発明は、
（１）酵母エキス溶液に、１）蓼藍由来「すくも」を用いた発酵建液、２）琉球藍由来の「泥藍」、３）インド藍から調製された「泥藍」を用いた発酵建液などのインジゴ還元能を有する組成物、合成インジゴ及びpH10用アルカリ緩衝液を添加・接触させた液を数日間保持し、この溶液を使用する藍染法である。
（２）ペプトン、コーンスチープリカー（CSL）、麦芽エキス、小麦ふすま（未処理）、小麦ふすま（脱脂）、小麦粉（強力粉）、小麦粉（薄力粉）、糖蜜、廃糖蜜など食品補助剤、家畜等の飼料、肥料として用いられている物質の溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させた後数日間保持し、この溶液を使用する藍染法である。 The gist of the present invention is as follows.
That is, the present invention
(1) Yeast extract solution: 1) Fermented building solution using “sukumo” derived from kumigo, 2) “Mudyan” derived from Ryukyu indigo, 3) Fermentation using “mud indigo” prepared from Indian indigo This is an indigo dyeing method in which a solution in which an indigo reducing ability composition such as a building fluid, a synthetic indigo and an alkaline buffer for pH 10 are added and contacted is retained for several days, and this solution is used.
(2) Food supplements such as peptone, corn steep liquor (CSL), malt extract, wheat bran (untreated), wheat bran (defatted), flour (strong flour), flour (soft flour), molasses, molasses, livestock, etc. A solution (or suspension) of a substance used as feed or fertilizer, a composition having indigo reduction ability such as 1), 2), or 3) of claim 1, synthetic indigo, and an alkaline buffer for pH 10 This is an indigo dyeing method in which this solution is kept for several days after adding and contacting.

（３）請求項１乃至請求項２の物質２つ以上を混ぜた溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させた後数日間保持し、この溶液を使用する藍染法である。
（４）請求項１乃至請求項３記載の物質の溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物と合成インジゴを添加・接触後、木灰（抽出液を含む）と石灰でpH 10〜11に調節しながら後数日間保持し、この溶液を使用する藍染法である。 (3) A composition or synthesis comprising an indigo reducing ability such as 1), 2), or 3) of claim 1 in a solution (or suspension) in which two or more substances of claims 1 to 2 are mixed. This is an indigo dyeing method in which indigo and an alkaline buffer for pH 10 are added and contacted and kept for several days and this solution is used.
(4) The composition (1), (2), (3), etc. having the ability to reduce indigo and synthetic indigo are added to the solution (or suspension) of the substance according to any one of claims 1 to 3. This is an indigo dyeing method in which the solution is used after being kept for several days while being adjusted to pH 10-11 with wood ash (including extract) and lime after contact.

本発明により、簡単な操作で染色が可能になり、かつ従来の発酵建法に比べ遜色ない染色が可能である。また従来（伝統的な）の発酵建液は、独特の臭い（不快臭）が生じているが、本方法によればかなり軽減されるなど従来の方法に比べ優れている点が多くその利用が期待できる。   According to the present invention, dyeing can be performed by a simple operation, and dyeing comparable to the conventional fermentation construction method is possible. In addition, the conventional (traditional) fermented building fluid has a unique odor (unpleasant odor), but it has many advantages over the conventional method, such as being considerably reduced by this method. I can expect.

以下、本発明について詳細に説明する。
先ず本発明は酵母エキスなどの溶液（あるいは懸濁液）に、インジゴ還元能を有する発酵建液等組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させ数日間保持することにより、藍染めを可能にした。
本発明に使用する発酵建液は、茨城県桜川市真壁町（真壁藍工房、蓼藍由来の「すくも」）、沖縄県名護市（やまあい工房、藍染茶房 藍風；琉球藍由来の「泥藍」）、沖縄県石垣島（島藍、インド藍由来の「泥藍」）であるが、これ以外の場所や地域で使用されている発酵建液ならなんでもよい。また偶然得られる液でもインジゴ還元能を有する組成物なら何でもよい。 Hereinafter, the present invention will be described in detail.
First, in the present invention, a solution (or suspension) such as yeast extract is added and brought into contact with a composition such as a fermented building fluid having indigo reduction ability, a synthetic indigo and an alkaline buffer for pH 10, and held for several days. Made indigo dyeing possible.
The fermented liquid used in the present invention is Makabe-cho, Sakuragawa City, Ibaraki Prefecture (Makabe Ai Kobo, “Sukumo” derived from Kanai), Okinawa Prefecture Nago City (Yamaai Kobo, Aizen Chabo Ai Kaze; Indigo ”), Ishigaki Island, Okinawa Prefecture (“ Mid Indigo ”derived from Indigo indigo and Indian indigo), but any fermented building fluid used in other places and regions may be used. In addition, the liquid obtained by chance may be any composition having indigo reduction ability.

本発明では合成インジゴを使用しているが、合成インジゴ以外の「すくも」、「泥藍」等インジゴ成分を含んだものなら何で使用できる。
また本発明では酵母エキス、ペプトン、コーンスチープリカー（CSL）、麦芽エキス、あるいは小麦ふすま（未処理）、小麦ふすま（脱脂）、小麦粉（強力粉）、小麦粉（薄力粉）などを使用しているが、糖蜜、廃糖蜜、食品補助剤、飼料、肥料等も使用でき、これらを単独あるいは数種類混合して使うことも可能である。これら物質の溶解（あるいは懸濁液）に使用するアルカリ緩衝液のpHは 10が好ましいが、それ以外のアルカリ側のpHでもよい。溶液のpH調整は、アルカリ緩衝（１ＭのNaHCO3／Na2CO3）を10 %加えることによりpH 10に調節できる。実施例には示していないが、溶液のpH調整に、木灰(抽出液を含む)または石灰あるいは両者を組み合わせてpHを10〜11に調整して使用することも可能である。木灰（抽出液を含む）と石灰を組み合わせてのpH調節は天然素材をベースとしている従来の発酵建法と同じ操作で行なうことになるので、同様の効果が期待できる。 In the present invention, synthetic indigo is used. However, any compound containing indigo components other than synthetic indigo such as “sukumo” and “mud indigo” can be used.
In the present invention, yeast extract, peptone, corn steep liquor (CSL), malt extract, or wheat bran (untreated), wheat bran (defatted), flour (strong flour), flour (weak flour), etc. are used. Molasses, molasses, food supplements, feed, fertilizer, etc. can be used, and these can be used alone or in combination. The pH of the alkaline buffer used for dissolving (or suspending) these substances is preferably 10, but other alkaline pH may be used. The pH of the solution can be adjusted to pH 10 by adding 10% alkaline buffer (1M NaHCO 3 / Na 2 CO 3 ). Although not shown in the examples, it is also possible to adjust the pH of the solution by adjusting the pH to 10 to 11 by using wood ash (including the extract) or lime or a combination of both. Since pH adjustment by combining wood ash (including extract) and lime is performed by the same operation as a conventional fermentation construction method based on natural materials, the same effect can be expected.

次に、本発明の藍染法について説明する。本発明の藍染法は、酵母エキスなどの溶液（あるいは懸濁液）に、発酵建液等のインジゴ還元能を有する組成物、合成インジゴ、pH 10調整用アルカリ緩衝液を添加・接触させることを特徴とする。
なお、反応液のpHは10が好ましいが、アルカリ性であればいずれのpHでもよい。本発明の溶液を用いることによって、合成インジゴだけでなく天然藍由来の「すくも」や「泥藍」から生地の染色が可能となる。 Next, the indigo dyeing method of the present invention will be described. The indigo dyeing method of the present invention involves adding and contacting a solution (or suspension) such as a yeast extract with a composition having indigo reduction ability such as a fermentation liquid, synthetic indigo, and an alkaline buffer for adjusting pH 10. Features.
The pH of the reaction solution is preferably 10, but any pH may be used as long as it is alkaline. By using the solution of the present invention, not only synthetic indigo but also “sukumo” and “mud indigo” derived from natural indigo can be used to dye the dough.

以下、実施例により本発明を具体的に説明する。但し、本発明はこれらの実施例にその技術範囲が限定されるものではない。なお、以下の実施例において%は、特に断りのない限り質量%を表す。
［実施例１］
茨城県桜川市真壁町の真壁藍工房で調製された「すくも」原料として用いた発酵建液を使用した。
まずRCM培地（Reinforced Clostridial Medium 培地、Difco社製：38 gを900 mlの蒸留水に溶解）9mlをオートクレ−ブで滅菌後、別滅菌したアルカリ緩衝液1ml｛（１ＭのNaHCO3／Na2CO3）を加えｐＨ10に調製するための液｝の入った（内径15 mmの）試験管に発酵建液を0.3 ml加えた後、30℃で5日間保持した。この液はインジゴ還元能を有しており、これを発酵建液組成物とした。 Hereinafter, the present invention will be described specifically by way of examples. However, the technical scope of the present invention is not limited to these examples. In the following examples, “%” represents “% by mass” unless otherwise specified.
[Example 1]
The fermented building fluid used as the raw material for “sukumo” prepared at Makabe Ai Kobo in Makabe-cho, Sakuragawa City, Ibaraki Prefecture was used.
First, 9 ml of RCM medium (Reinforced Clostridial Medium medium, manufactured by Difco: 38 g dissolved in 900 ml of distilled water) was sterilized by autoclaving and then separately sterilized alkaline buffer 1 ml {(1 M NaHCO 3 / Na 2 CO 3 ) was added, and 0.3 ml of the fermentation solution was added to a test tube (with an inner diameter of 15 mm) containing a solution for adjusting to pH 10}, and then kept at 30 ° C. for 5 days. This liquid has indigo reduction ability, and this was used as a fermentation building liquid composition.

次に（内径23 mmの）試験管に、酵母エキス（1%）、ペプトン（1%）、コーンスチープリカー｛CSL、2% (v/v）)、麦芽エキス（１％）、小麦ふすま（未処理、5%）、小麦ふすま（脱脂、5%）、小麦粉（強力粉、5%）、小麦粉（薄力粉、5%）、糖蜜（5％）、廃糖蜜（５％）を各々入れた後、蒸留水45 mlに溶解（あるいは懸濁）し、さらに合成インジゴ（0.2%）と液を均一にするための回転子（撹拌子、径15 mm）入れた後オートクレーブで滅菌した。これにアルカリ緩衝液5 ml（１ＭのNaHCO3／Na2CO3）を加えｐＨ10に調製するための液｝を加え50 mlにした。この液に発酵建液由来液1.5 mlを添加・接触させた後、30℃で5日間保持した。保持期間中毎日１度はスターラーで液を撹拌した。この溶液に生地を5分間及び2日間漬けて、空気に曝し可溶化したインジゴを元に戻してから水洗した。藍染めによる染色の程度を比較した結果、表１に示したように酵母エキス、ペプトン、CSL、麦芽エキス、小麦ふすま（未処理）を用いた溶液で染色した生地の染色度が優れていた。インジゴ染色が優れた酵母エキス、ペプトン、CSL、麦芽エキスなどは5分間の浸漬で十分比較できたが、それ以外の着色の弱い溶液の比較は2日間浸漬したもので補った。
溶液の臭気（不快臭）は、小麦粉（薄力粉）を用いたときが最も強く、小麦粉（強力粉）、小麦ふすま（脱脂）の順になっていた。一方酵母エキス、CSL、麦芽エキス、糖蜜、廃糖蜜を用いたときはかなり臭いも弱く、特に麦芽エキスではインジゴ還元能は若干劣っていたが、他の物質を使用したときと比べ不快臭がほとんど無かった。 Next, in a test tube (with an inner diameter of 23 mm), yeast extract (1%), peptone (1%), corn steep liquor (CSL, 2% (v / v)), malt extract (1%), wheat bran ( Untreated, 5%), wheat bran (defatted, 5%), flour (strong flour, 5%), flour (weak flour, 5%), molasses (5%), molasses (5%), After dissolving (or suspending) in 45 ml of distilled water, a synthetic indigo (0.2%) and a rotor (stirring bar, diameter 15 mm) for homogenizing the solution were added, and then sterilized by an autoclave. To this was added 5 ml of an alkaline buffer (1M NaHCO 3 / Na 2 CO 3 ) and a solution for adjusting to pH 10} to make 50 ml. After 1.5 ml of the fermentation building liquid-derived liquid was added to and contacted with this liquid, it was kept at 30 ° C. for 5 days. The liquid was stirred with a stirrer once a day during the holding period. The dough was dipped in this solution for 5 minutes and 2 days, and the indigo solubilized by exposure to air was returned to its original state and washed with water. As a result of comparing the degree of dyeing by indigo dyeing, as shown in Table 1, the dyeing degree of the dough dyed with a solution using yeast extract, peptone, CSL, malt extract, wheat bran (untreated) was excellent. Yeast extract, peptone, CSL, malt extract, etc., which were excellent in indigo dyeing, could be compared sufficiently by soaking for 5 minutes, but other weakly colored solutions were compared with those soaked for 2 days.
The odor (unpleasant odor) of the solution was strongest when flour (weak flour) was used, and was in the order of flour (strong flour) and wheat bran (defatted). On the other hand, when yeast extract, CSL, malt extract, molasses, and molasses were used, the odor was rather weak. Especially, malt extract had slightly indigo reduction ability, but there was almost no unpleasant odor compared with other substances. There was no.

［実施例２］
偶然得られたインジゴ還元能を有する液を用いた。
本来インジゴ還元能がない系で基準培地（blank）として用いた液、すなわち内径23 mmの大型試験管に合成インジゴ（0.01%と0.2%をそれぞれ添加）とRCM培地（Reinforced Clostridial Medium 培地、Difco社製：38 gを900 mlの蒸留水に溶解）45 mlを入れオートクレ−ブ滅菌後、別滅菌したアルカリ緩衝液5ml（１ＭのNaHCO3／Na2CO3）を加えｐＨ10に調整したもので、無菌操作を行った後、30℃で5日間（毎日一度は回転子を回すことにより液を均一化した）保持したところ、インジゴ還元能が認められた。これは外部から偶然得られたものと考えられ、インジゴ還元能を有する液とした。 [Example 2]
A liquid having the ability to reduce indigo obtained by chance was used.
Originally used as a reference medium (blank) in a system that does not have indigo reduction ability, that is, synthetic indigo (0.01% and 0.2% added respectively) and RCM medium (Reinforced Clostridial Medium medium, Difco) Manufactured: 38 g dissolved in 900 ml of distilled water) 45 ml was added and autoclaved, and then 5 ml of another sterilized alkaline buffer (1M NaHCO 3 / Na 2 CO 3 ) was added to adjust the pH to 10, After performing aseptic operation, the indigo reducing ability was recognized when it was kept at 30 ° C. for 5 days (the liquid was homogenized by rotating the rotor once a day). This was considered to have been obtained by chance from the outside, and a liquid having indigo reduction ability was obtained.

次に合成インジゴ（0.01%）の入った（内径15 mmの）試験管にRCM培地（Reinforced Clostridial Medium 培地、Difco社製：38 gを900 mlの蒸留水に溶解）9mlを入れた後オートクレ−ブで滅菌した。これに滅菌したアルカリ緩衝液1ml（１ＭのNaHCO3／Na2CO3）を加えｐＨ10に調製したもの｝を加えたものにインジゴ還元能を有する液0.3 mlを入れた後、30℃で5日間静置した。そしてこの液をインジゴ還元能を有する組成物とした。 Next, 9 ml of RCM medium (Reinforced Clostridial Medium medium, manufactured by Difco: 38 g dissolved in 900 ml of distilled water) was placed in a test tube containing synthetic indigo (0.01%) (with an inner diameter of 15 mm) and then autoclaved. Sterilized with To this was added 1 ml of sterilized alkaline buffer (1M NaHCO 3 / Na 2 CO 3 ) and adjusted to pH 10}, and 0.3 ml of an indigo reducing solution was added, and then at 30 ° C. for 5 days. Left to stand. This liquid was used as a composition having indigo reduction ability.

さらに実施例１でインジゴ還元力の優れた酵母エキスを用いて以下の検討を加えた。すなわち合成インジゴ（0.2%）の入った内径23 mmの大型試験管に、酵母エキス（45mlの蒸留水に酵母エキス0.5 gを溶かした）を入れオートクレーブ滅菌後、アルカリ緩衝液5ml（１ＭのNaHCO3／Na2CO3）を加えｐＨ10に調整した液にインジゴ還元力を有する液1.5 mlを加えた後、予め加えた回転子を回して毎日一度反応液を均一にしながら、30℃で5日間静置した。そしてこの反応液に生地を５分間浸漬後、空気に曝して可溶化したインジゴを元のインジゴに戻してから水洗した。その結果、表２に示すように実施例１と同様に生地が顕著に着色していることが認められた。 Furthermore, the following examination was added using the yeast extract excellent in indigo reduction power in Example 1. In other words, in a large test tube with an inner diameter of 23 mm containing synthetic indigo (0.2%), yeast extract (0.5 g of yeast extract was dissolved in 45 ml of distilled water) was sterilized by autoclave, and then 5 ml of alkaline buffer (1 M NaHCO 3). / Na 2 CO 3 ) and add 1.5 ml of indigo reducing solution to the solution adjusted to pH 10, and turn the rotor added in advance to make the reaction solution uniform once a day. I put it. The dough was immersed in this reaction solution for 5 minutes, and the indigo solubilized by exposure to air was returned to the original indigo and washed with water. As a result, as shown in Table 2, it was confirmed that the dough was remarkably colored as in Example 1.

［表１］

Figure 2008274488
酵母エキスなどの溶液（あるいは懸濁液）に、真壁藍工房で調製された発酵建液組成物、合成インジゴ及びpH 10調整用アルカリ緩衝液を添加・接触させた後30℃で静置した溶液に、生地を５分間と２日間浸漬することによるインジゴ還元能（染色度）を比較した示した表である。なお、インジゴ還元能（染色度）の強さを＋から＋＋＋の３段階で表した。＋は２日間浸漬により染色が見られたもので、＋＋＋は５分間の浸漬で染色が十分に見られた。なお＋（w）は２日間の浸漬でやっと染色が見られたものを示した。 [Table 1]
Figure 2008274488
A solution (or suspension) of yeast extract or the like added with and added to the fermented building fluid composition prepared at Makabe Ai Kobo, synthetic indigo, and an alkaline buffer for pH 10 adjustment, and then allowed to stand at 30 ° C. 2 is a table comparing indigo reducing ability (staining degree) by dipping the dough for 5 minutes and 2 days. The strength of indigo reduction ability (staining degree) was expressed in three stages from + to ++. + Was dyed by immersion for 2 days, and +++ was sufficiently dyed by immersion for 5 minutes. In addition, + (w) showed what was finally dye | stained by immersion for 2 days.

［表２］

Figure 2008274488
酵母エキス溶液に、インジゴ還元能を有する組成物、合成インジゴ（0.2%）及びpH 10調整用アルカリ緩衝液を添加・接触した後、30℃で数日間保持した反応液を用いて生地の着色程度をインジゴ還元能（染色度）として示した。なお、インジゴ還元（染色）の強さを＋から＋＋＋の３段階で表したが、＋＋＋は５分間の浸漬で染色が十分に見られたものを示した。 [Table 2]
Figure 2008274488
The degree of coloring of the dough using the reaction solution kept at 30 ° C for several days after adding and contacting the indigo reducing composition, synthetic indigo (0.2%) and alkaline buffer solution for pH 10 adjustment to the yeast extract solution Was shown as indigo reduction ability (staining degree). The intensity of indigo reduction (dyeing) was expressed in three stages from + to +++. +++ indicates that the dyeing was sufficiently observed after immersion for 5 minutes.

Claims (4)

酵母エキス溶液に、１）蓼藍由来「すくも」を用いた発酵建液、２）琉球藍由来の「泥藍」、３）インド藍から調製された「泥藍」を用いた発酵建液などのインジゴ還元能を有する組成物、合成インジゴ及びpH10用アルカリ緩衝液を添加・接触させた液を数日間保持し、この溶液を使用する藍染法。   Yeast extract solution: 1) Fermented building fluid using “Sukumo” derived from kyanai, 2) “Mudyan” derived from Ryukyu indigo, 3) Fermented building fluid using “mud indigo” prepared from Indian indigo, etc. Indigo dyeing method in which a solution in which an indigo reducing ability composition, synthetic indigo, and an alkaline buffer for pH 10 are added and contacted is retained for several days, and this solution is used. ペプトン、コーンスチープリカー（CSL）、麦芽エキス、小麦ふすま（未処理）、小麦ふすま（脱脂）、小麦粉（強力粉）、小麦粉（薄力粉）、糖蜜、廃糖蜜など食品補助剤、家畜等の飼料、肥料として用いられている物質の溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させた後数日間保持し、この溶液を使用する藍染法。   Food supplements such as peptone, corn steep liquor (CSL), malt extract, wheat bran (untreated), wheat bran (defatted), flour (strong flour), flour (weak flour), molasses, molasses, animal feed, fertilizer Add a composition (1), 2), 3), etc. having indigo reduction ability, synthetic indigo and alkaline buffer for pH 10 to the solution (or suspension) of the substance used as Indigo dyeing method using this solution for several days after contact. 請求項１乃至請求項２の物質２つ以上を混ぜた溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物、合成インジゴ及びpH 10用アルカリ緩衝液を添加・接触させた後数日間保持し、この溶液を使用する藍染法。   A composition (or suspension) in which two or more substances of claim 1 or 2 are mixed, a composition having indigo reduction ability such as 1), 2) and 3) of claim 1, a synthetic indigo and pH A method of indigo dyeing, in which an alkaline buffer solution for 10 is added and kept in contact for several days and then used. 請求項１乃至請求項３の物質の溶液（あるいは懸濁液）に、請求項１の１）、２）、３）などのインジゴ還元能を有する組成物と合成インジゴを添加・接触後、木灰（抽出液を含む）と石灰でpH 10〜11に調節しながら後数日間保持し、この溶液を使用する藍染法。   After adding / contacting the indigo-reducing composition such as 1), 2), 3) and the like to the solution (or suspension) of the substance of claims 1 to 3, wood ash Indigo dyeing using this solution, which is kept for several days while adjusting to pH 10-11 with lime (including extract).
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008274489A (en) * 2007-04-27 2008-11-13 National Institute Of Advanced Industrial & Technology Method for bleaching indigo-dyed fabric
KR101104820B1 (en) * 2011-07-11 2012-01-16 전남대학교산학협력단 Reduction method of indigo
KR101173638B1 (en) 2010-09-16 2012-08-13 허북구 Composition for fermentation and dyeing using indigo and package comprising the composition
KR101249252B1 (en) 2011-04-01 2013-04-01 재단법인나주시천연염색문화재단 Bacterial strain having indigo reduction ability and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10237341A (en) * 1997-02-24 1998-09-08 Sakamoto Denimu Kk Continuous indigo dyeing of textile material using natural indigo dye
JP2005270028A (en) * 2004-03-25 2005-10-06 National Institute Of Advanced Industrial & Technology Indigo-reducing microorganism and method for reducing indigo by using the same
JP2008274489A (en) * 2007-04-27 2008-11-13 National Institute Of Advanced Industrial & Technology Method for bleaching indigo-dyed fabric

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10237341A (en) * 1997-02-24 1998-09-08 Sakamoto Denimu Kk Continuous indigo dyeing of textile material using natural indigo dye
JP2005270028A (en) * 2004-03-25 2005-10-06 National Institute Of Advanced Industrial & Technology Indigo-reducing microorganism and method for reducing indigo by using the same
JP2008274489A (en) * 2007-04-27 2008-11-13 National Institute Of Advanced Industrial & Technology Method for bleaching indigo-dyed fabric

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008274489A (en) * 2007-04-27 2008-11-13 National Institute Of Advanced Industrial & Technology Method for bleaching indigo-dyed fabric
KR101173638B1 (en) 2010-09-16 2012-08-13 허북구 Composition for fermentation and dyeing using indigo and package comprising the composition
KR101249252B1 (en) 2011-04-01 2013-04-01 재단법인나주시천연염색문화재단 Bacterial strain having indigo reduction ability and uses thereof
KR101104820B1 (en) * 2011-07-11 2012-01-16 전남대학교산학협력단 Reduction method of indigo
WO2013009036A2 (en) * 2011-07-11 2013-01-17 전남대학교산학협력단 Method for reducing indigo
WO2013009036A3 (en) * 2011-07-11 2013-03-14 전남대학교산학협력단 Method for reducing indigo
CN103842448A (en) * 2011-07-11 2014-06-04 全南大学校产学协力团 Method for reducing indigo

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