JP2008263946A - Addition to food of nobiletin contained in skin of fruit of citrus depressa by quantification - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To add nobiletin contained in citrus depressa to food products other than the citrus depressa with controlling its quantity, because existing products are not products to which a controlled quantity of nobiletin is added but are 100% citrus depressa fruit, while a fundamental study reports actions of nobiletin contained in the citrus depressa Hayata of cancer prevention/cancer metastasis prevention/anti-inflammation/osteoporosis or periodontitis prevention/blood sugar level and blood pressure suppression and the like as a functional component and the commercial value of existing juice of citrus depressa, flavonoid extract tablets of citrus depressa Hayata and the like is improved by indicating nobiletin. <P>SOLUTION: The skin of fruit of citrus depressa having a high nobiletin content is dried and nobiletin is extracted by Awamori. The amount of nobiletin in the extraction liquid is measured and the extraction liquid as a stock solution is added to different food products. (Case 1) Addition to Awamori: Since the extraction solution is Awamori, addition to Awamori is performed by a method in which the stock solution is diluted with Awamori not containing nobiletin. (Case 2): Addition to rice porridge/tea: The Awamori solution containing nobiletin is boiled for removing ethyl alcohol of Awamori and is added to rice porridge or tea. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to the production of a nobiletin-containing food, characterized in that nobiletin contained in the seeker skin is extracted with awamori, the amount of nobiletin in the extract is manipulated, and a prescribed amount of nobiletin is added to the food.

  It has been reported that citrus fruits contain various functional ingredients. Nobiletin, one of the functional ingredients, has been applied for patents (2001-39660) and patents together with basic research such as cancer prevention, cancer transfer inhibition action, anti-inflammatory action, prevention of osteoporosis and periodontitis, blood glucose level and blood pressure suppression action (No. 3010210) has been reported, and product development has attracted attention.

  It is confirmed that Shikuwasa native to Okinawa contains more nobiletin than other citrus fruits. In the product “SEIKUSAR flavonoid extract granules”, it is introduced that the fruit skin contains more than 400 times the fruit juice, and the product name is “NOBILETIN-CONTAINING FOOD”, which is expected to increase the added value of SEIKU-SAA.

  Seek juicer juice products on the market appear to be similar and are judged by the amount of nobiletin to determine. Since nobiletin has been researched for its effectiveness in preventing, inhibiting, suppressing and preventing various modern diseases, the product value is enhanced by displaying the amount of nobiletin on the product together with the name of the seeker.

Nobiletin-containing foods and contents of ready-made Okinawa-made foods for Shikuwasa (1) Shikuwasa flavonoid extract granules
Ingredients ... Ogimi Village Seaquaser Skin
Nobiletin content: 0.05 mg (per 200 mg per capsule)
(2) Katsuyama seeker
Ingredients ... Sikhwasa juice (straight)
Nobiletin content ... 31mg / 100ml
(3) Gitto seeker 500ml
Ingredients ... Sikhwasa juice (straight)
Nobiletin content ... 8.4mg / 100ml
The above-mentioned ready-made product is a seeker fruit itself (100%) and not an additive.

  The title of the patent publication 2005-145824 invention “Method for producing nobiletin” is to extract only nobiletin by extracting nobiletin with ethanol and eluting nobiletin adsorbed on the porous adsorption resin.

The ready-made product is the product of the squeak juicer juice or the remaining residue after squeezing the juice, and the product made from the squeak juicer necessarily contains nobiletin.
The problem to be solved is to extract nobiletin contained in the seeker skin, so that the nobiletin can be quantitatively added to food.

  Nobiletin in the pericarp is extracted when the shikwasa pericarp is dried and soaked in Awamori. When 100 g of dried fruit skin is soaked in Awamori at 30 degrees, the immersion liquid will not rot even for a long time. In addition, 30-degree Awamori is a general Awamori frequency and is inexpensive.

  About 1 month after soaking the dried pericarp in awamori, the amount of nobiletin in the soaking solution is quantitatively analyzed by liquid chromatography. This quantitative analysis is in the liver and kidneys and asks a specialized institution to obtain accurate values. The amounts of nobiletin of the present invention are all the results of analysis requested to specialized institutions.

  The analysis results will arrive within 2 weeks from the specialized institution. Details [0015] [Table 2] showing the amount of nobiletin extracted from 100 g of dried pericarp to 1800 ml of quantitative awamori. Addition to food is calculated by dilution and proportional distribution based on the prescribed amount, and blended to make a food that clearly specifies the amount of nobiletin [0017] [0018] [Example].

  Knowing the amount of nobiletin extracted from 100 g of dried pericarp to 1800 l of quantitative Awamori, the amount of raw pericarp that can form 100 g of dried pericarp is known, so the amount of nobiletin per 1 g of raw pericarp can also be calculated. According to this, the amount of nobiletin in the method of extracting from quasar dried pericarp at 30 degrees awamori is larger than the amount published by research institutions so far [Details] [Table 2].

As a result of boiling a nobiletin-containing awamori solution in which the amount of nobiletin is known, measuring the amount of nobiletin again, and comparing the amount of nobiletin before boiling, it was found that the amount of nobiletin did not change. This showed that nobiletin was not destroyed by heat.
Details [0020] [Table 4].

  Since nobiletin is not broken by heat, if the nobiletin-containing awamori solution is heated, alcohol can be removed while preserving the amount of nobiletin. That is, the nobiletin-containing awamori solution can be made into a nobiletin-containing aqueous solution. If this nobiletin-containing aqueous solution is used, it can be added to other types of foods that do not require alcohol. For example, rice cake and tea.

Nobiletin content in shikwasa peel

As can be seen from the measurement results [Table 1], it can be seen that a large amount of nobiletin in Shikwasa fruit is contained in the peel.

Item A in [Table 2] is the measurement result. The B term is calculated from the measurement result of the A term.
Shikuwasa blooms in March and April, and the fruits begin to turn in October, and most fruits turn to ripe in December. The amount of nobiletin to be extracted is a fruit at the time of full maturity, and the work of peeling the peel is easier than the young fruit of June.
By the way, the amount of nobiletin in 1 g of raw pericarp contains a lot of immature in June, and it seems that the amount of no ripeness in December is less due to the water content of the fruit. In other words, the nobiletin amount of Sikhwasa fruit may not change much.
In addition, according to the data of the Okinawa Prefectural Agricultural Experiment Station Nago Branch Fruit Tree Laboratory, the nobiletin content of “Ogimi Kugani”, which contains the most amount of nobiletin, is 2.2 mg / g, whereas the fruit of this sample in December However, the extraction amount is 3.48 mg / g, which is about 1.5 times higher. This is an interesting topic whether it depends on the variety of the seeker, the extraction method or the analysis method.

Immersion period and extraction amount

[Table 3] The results are obtained by immersing 100 g of dried pericarp in 1800 ml of whipping volume and measuring the amount of extracted nobiletin in immersion periods of 2 weeks and 4 weeks. The difference is small at 2.79 mg / 100 ml, so it is considered that it is almost extracted in 4 weeks.

Nobiletin added to food

The commercialized sukuwaser flavonoid extract granules are ingesting 5 to 10 grains daily in anticipation of the blood sugar level of the bioactive action of nobiletin and the antihypertensive action and antihypertensive action. When the amount of nobiletin is converted from the instructions, it becomes 0.25 mg to 0.5 g.
In addition, nobiletin is taken up on the homepage of the Dietary Information Service Center “Fruit Component with High Carcinogenic Inhibition Effect”, and the effective concentration of carcinogenesis suppression is about 100 ppm = 10 mg / 100 ml by dietary administration.
The amount and efficacy of ready-made nobiletin are not found. Efficacy is expected as a minor component.

How to make "Awamori with nobiletin"

In the concept of diluting 87.73mg / 100ml of stock solution extracted in 4 weeks in December skin peel to 1.0mg / 100ml, if you add how many awamori to 87.73mg / 100ml, it will be diluted to 1.0mg / 100ml Calculate with the idea of whether or not.
If the amount X of awamori is added, the calculation formula is 87.73 / (100 + X) = 1/100, and when X is obtained, X = 8673. When 8673 ml of awamori is added to 100 ml of the stock solution, 8773 ml of 1.0 mg / 100 ml of nobiletin-containing awamori is produced.
Also, if the stock solution can be made from 100 ml to 8773 ml, in order to make 1000 ml of 1.0 mg / 100 ml, the stock solution and awamori can be obtained by calculating the proportional distribution of how many ml each.

(1) Problem: Using the stock solution 103.1mg / 100ml, make 1100ml of nobiletin 2mg / 100ml.
{Circle around (2)} Calculation: Formula 103.1 / (100 + X) = 2/100 When Awamori X = 5055 ml to be added and 100 ml of the stock solution are mixed together, 5155 ml of a concentration of 2 mg / 100 ml can be obtained.
If 5155 ml can be obtained from 100 ml of the stock solution, the stock solution X for making 1100 ml of 2 mg / 100 ml is X = 1100/5155 × 100 = 21.3 ml.
Moreover, the amount Y of foaming to add becomes Y = 1100-21.3 = 1078.7 ml.
(3) Verification example: With reference to the calculation in (2), 20 ml of the stock solution and 990 ml of Awamori were mixed to make 1100 ml, and the amount of nobiletin was measured. As a result, a result of 1.89 mg / 100 ml was obtained. The calculation results in 1.87 mg / 100 ml.

Summary of the process of making nobiletin awamori

Manufacture of nobiletin-containing foods containing seeker

Nobiletin is stable without being broken by heat. [Table 4]

Prototype “Nobiletin 5 mg / 100 ml porridge” In order to make 5 times the koji of nobiletin 5 mg / 100 ml, rice 1 cup = 150 g, water 5 cup = 800 g, nobiletin-containing aqueous solution 103 mg / 100 ml = 50 ml, that is, nobiletin 51. Put 5mg and cook. The weight before cooking was about 1000g, and the weight after cooking was 820g.
30 g of this porridge was soaked in 540 ml of Awamori for 2 weeks, and the amount of nobiletin in this soaking solution was measured. The result is 0.28 mg / 100 ml, 570/100 × 0.28 = 1.596 mg in bowl 30 g 100/30 × 1.596 = 1.32 mg / 100 g in bowl 100 g, whole bowl Of 820 g is 5.32 mg × 8.2 = 43.6 mg. This is 7.9 mg less than the initial 51.5 mg, but it has been verified that nobiletin is preserved even during heating in the process of making porridge.

Securing materials

100 g of dried shikwasa peel is made from about 400 g of full-ripe raw peel, and there are about 53 fruits for this purpose. In addition, it is easy to secure awamori as a special product of Okinawa Prefecture, and the amount of nobiletin in the awamori extract can be preserved [Table 5], and a large amount of nobiletin-containing awamori solution can be prepared in the season when fruits are harvested. By the way, the amount of nobiletin when stored for a long period of time in the dry peel decreases (Table 6).

Production volume

  The concentration of nobiletin extract in the skin of October and December is about 81 mg / 100 ml on average. From 100 ml of this stock solution, 8100 ml of “Nobiletin 1.0 mg / 100 ml Awamori” can be made. If this is packed in 1800ml bottle, it will be 4.5 bottles. If 1400 ml of the stock solution made for each bottle is taken, 4.5 × 14 = 63, that is, 63 bottles. If the dried skin is 1 kg, the stock solution can be increased 10 times, so there are 630 bottles per bottle. If the concentration of nobiletin is halved to 0.5 mg / 100 ml, the number of bottles will be doubled to 1260 bottles.

  Addition to foods other than awamori requires a device such as using a nobiletin-containing awamori solution (stock solution) in advance by removing alcohol and using it as a nobiletin-containing aqueous solution.

Since nobiletin is stable to heat, it can be added to most foods.
For example, awamori (shochu), rice cake, drinking water such as tea, and cakes.
By the way, even when the nobiletin-containing awamori solution is added to a trace amount of food having a nobiletin concentration of 1 mg / 100 ml, the taste and aroma of the original food are lost, so a device to eat deliciously is necessary. In Awamori with nobiletin, it is delicious to make a cocktail with lemon water.

Demand aspect

Experiment

Nobiletin passes through the semipermeable membrane.
Materials: Semi-permeable membrane uses cellophane, aqueous solution containing nobiletin, water, petri dish

result

  Petri dish A was placed in contact with 100 ml of water and 100 ml of nobiletin-containing aqueous solution with cellophane as the boundary. Petri dish B was in the state 24 hours later, and the water portion of petri dish A was 89 ml, and nobiletin was contained at a concentration of 11 mg / 100 ml. The portion of the nobiletin-containing aqueous solution increased to 110 ml.

Consideration

  The results indicate that nobiletin is the size of a molecule that can pass through cellophane. Nobiletin taken from the mouth is thought to be absorbed into the body through the intestine, which is a semipermeable membrane. Therefore, the method of adding nobiletin to food and taking it by mouth makes sense.

The purpose of adding nobiletin to foods using a nobiletin-containing awamori solution as a raw material is because it expects the efficacy of the basic research results of nobiletin so far.
Currently, the amount of nobiletin intake is very small, but the appropriate amount is unknown. The characteristic of nobiletin-containing awamori solution is that the amount of nobiletin can be adjusted, so that nobiletin-added food can be made to find the appropriate amount for ingestion, and animal experiments and clinical tests can be conducted.

This nobiletin amount can be adjusted and distributed even with dried pericarp.
There are also 12.60 mg to 15.79 mg per gram of dried pericarp [0015] Item B (e), which can be distributed to foods that it is desirable to add in dry pericarp by adjusting the amount of nobiletin.

Claims (2)

About nobiletin containing squeaker,
A method for producing a nobiletin-containing awamori solution characterized by extracting the contained nobiletin by immersing the dried peel of sequoccer in awamori.   A method for producing a nobiletin-added food specifying the amount of nobiletin, characterized in that the nobiletin amount of the nobiletin-containing awamori solution is used as an index, the nobiletin is distributed and added to the food.