JP2008214299A - Composition for prevention, treatment, or amelioration of cancer containing water-soluble extract constituents of carica papaya and hedyotis diffusa - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition useful for the prevention, treatment, or amelioration of cancers which produces little side effects and is highly safe. <P>SOLUTION: The composition comprises a combination of a water-soluble extract constituent from parts (e.g. leaves) of Carica papaya with a water-soluble extract constituent from parts (e.g. leaves) of Hedyotis Diffusa. This combination can effectively achieve prevention, treatment, or amelioration of solid cancers such as stomach cancer, lung cancer, pancreatic cancer, and colon cancer, produces little side effects, is highly safe, and is therefore of great significance. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a composition for preventing, treating or ameliorating cancer comprising a water-soluble extract component of papaya and white flower serpentine. More specifically, it contains components extracted by roasting the leaves of papaya and white serpentine grass in boiling water, maintaining the immunostimulatory action of both components, and exhibiting a synergistic effect in antitumor activity, The present invention relates to a composition for prevention, treatment or amelioration, which can be used for medicine and food.

In particular, in the case of malignant tumors, chemotherapy is indispensable when it is impossible to remove the tumor by surgery alone or until the surgery itself is difficult to perform, as well as obtaining a guarantee of postoperative remission. However, it is difficult to increase tumor specificity in chemotherapy, and the present situation is that side effects on self-normal cells are always accompanied. Faced with side effects with exhausted physical strength is easily imagined to be a great deal of physical and mental damage, but no matter how painful the treatment is, the affected and the surrounding people will face all pain and difficulties, It is natural to connect the desire for a good way.
On the other hand, information on various alternative therapies is flooded in society. Among them, there are many things with poor credibility, those that impose an economic burden, those that are difficult to continuously ingest, etc., and even if it is unrealistic information, it is necessary to believe It is also true. However, the original alternative therapy is aimed at the treatment of diseases by a mechanism that cannot be elucidated by so-called modern medicine, and if scientific considerations do not always exist on the basis of empirical rules, the significance is recognized. Is difficult and cannot be hope for recovery.

It has been empirically known that plant-derived extracts that have been passed down since ancient times are effective for certain diseases. White flower snake tongue grass (Hedyotis Diffusa, Japanese name: Futabamugur), which has a long history as a Chinese herbal medicine, is a plant that grows in Japan as an annual plant of the Akane family, and there is relatively much information as a folk remedy. Antitumor effects, antiviral effects, IgE production suppression, antiparasitic effects, and the like have been reported by oral administration to mice (Non-patent Documents 1, 2, 3, and 4). In addition, Haneda lotus (Hancalen, Lamiaceae, Scutellaria Barbatae) is known as a Chinese medicine that exerts an anti-tumor effect, and the anti-tumor effect when white flower serpentine grass and Haneda lotus are combined is also examined. (Non-Patent Documents 5 and 6).
On the other hand, papaya (Carica Papaya) is now distributed throughout the tropics, and its leaves contain antioxidant vitamin C, tannin, vitamin A, E, K, saponin, iron, etc. In addition, an anti-inflammatory effect, a parasite-control effect and the like have been reported (Non-patent Document 7). Furthermore, it has been reported that the extract component of papaya leaves has an antitumor effect (Patent Document 1).
Yoshida Y, Wang MQ, Liu JN, Shan BE, Yamashita U. Immunomodulating activity of chinese medicinal herbs and oldenlandia diffusa in particular.Int J Immunopharmacol 1997; 19 (7): 359-70 Shan BE, Yoshida Y, Sugiura T, Yamashita U. Stimulating activity of chinese medicinal herbs on human lymphocytes in vitro. Int J Immunopharmacol 1999; 21 (3): 149-59 Lin CC, Ng LT, Yang JJ, Hsu YF. Anti-inflammatory and hepatoprotective activity of peh-hue-juwa-chi-cao in male rats. Am J Chin Med 2002; 30 (2-3): 225-34 Lin CC, Ng LT, Yang JJ. Antioxidant activity of extracts of peh-hue-juwa-chi-cao in a cell free system. Am J Chin Med 2004; 32 (3): 339-49 Wong BY, Lau BH, Wan CP, Oldenlandia diffusa and Scutellaria barbata augment macrophage oxidative burst and inhibit tumor growth, Cancer Biother Radiopharm 1996; 11 (1): 51-56 Yoshida Y, Wang MQ, Liu JN, Sha Yamashita U., Imunomodulating activity of Chinese medicinal herbs and Oldenlandia diffusa in particular, Int J Immunopharmacol 1997: 19 (7): 359-370 Hsu S. Green tea and the skin.J Am Acad Dermatol 2005; 52 (6): 1049-59 International Publication No. WO2006 / 004226A1 Pamphlet

It has been empirically known that plant-derived extracts that have been passed down since ancient times are effective for certain diseases, but the scientific basis is poor, and the mechanism of the effects is unclear. There are many parts. Plant-derived extracts that have been ingested by humans over a long period of time should have not only empirical wisdom but also have great potential for future drug development. In addition, if an extract obtained from white flower snake tongue grass, half-branching lotus, papaya, etc. exhibits effective medicinal effects, these plants are originally edible, so there are few side effects and a highly safe therapeutic agent. Can be expected.
Accordingly, an object of the present invention is to provide a highly safe composition having a high cancer prevention, treatment or amelioration effect and having few side effects, comprising a plant-derived extract as an active ingredient.

The present inventors focused on water-soluble extract components derived from white flower serpentine grass and papaya native to tropical America, which have a long history as Chinese herbal medicines, and conducted immunological analysis on these extracts. In comparison, the immunostimulatory effect was mainly shown on the water-soluble extract component of white flower serpentine, and the cytotoxic activity could be significantly enhanced on the water-soluble extract component of papaya. Furthermore, after examining the details of this possibility, it was hoped that a complementary or synergistic effect would be obtained by mixing the two, and as a result of analysis, the mixing of both maintained the immunostimulatory effect. However, it was confirmed that synergistic damage activity against tumor cell lines was induced. The present invention has been completed based on such findings.
Accordingly, the present invention is a composition for preventing, treating or improving cancer, comprising as an active ingredient a water-soluble extract component of papaya (Carica papaya) and a water-soluble extract component of Hedyotis Diffusa. is there.

  A composition comprising both a water-soluble extract component of papaya and a water-soluble extract component of white flower serpentine grass as an active ingredient maintains an immunostimulatory action and exhibits a synergistic damage activity against tumor cell lines. Therefore, the composition of the present invention is useful as a highly safe pharmaceutical, health food, etc. that has a high effect of preventing, treating or improving cancer and has few side effects. In addition, the water-soluble extract obtained by boiling is generally accepted as a “decoction” and can be ingested relatively easily regardless of the degree of the disease state. In addition, it is highly useful in that it has excellent safety, economic efficiency, and convenience because of its continuous consumption.

The present invention is described in detail below.
In the present invention, both the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine are used as active ingredients. The tissue of papaya and white flower serpentine used for obtaining the extract component may be any of leaves, roots, stems and fruits, but leaves are particularly preferable. The tissue of these papaya and white flower serpentine is dried, the resulting dried product is added to water or hot water and boiled for a long time, and the resulting decoction is used as an active ingredient. Moreover, it is also possible to obtain a decoction liquid by boiling it by adding it to water or hot water as it is without drying tissues such as leaves of papaya and white flower serpentine grass. More specifically, for example, the leaves of papaya and white flower serpentine grass are usually left to stand for 1 to 2 days under sunlight to obtain a dried product. A quantity of about 5 to 100 g of this dried leaf is usually added to 50 to 1000 ml, preferably 100 to 500 ml of water or hot water, and usually boiled while boiling for 30 minutes to 5 hours, preferably 1 to 2 hours. Is preferred. The container used for boiling is preferably a glass, wooden or plastic container that is not a metal container. After boiling, it is preferable to keep the temperature at about 60 ° C. to 70 ° C. and to concentrate to about 50 ml to 250 ml. Next, after filtering and removing the leaves, it is preferable to maintain the temperature at about 60 ° C. to 70 ° C. and to concentrate to about 25 ml to 125 ml. Next, the mixture is centrifuged with a high-speed centrifuge, and the supernatant is collected and passed through a plurality of sterile filtration filters having different pore diameters. Ingredients can be preferably obtained.

  The composition of the present invention can be obtained by mixing both the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine that are thus obtained. The mixing ratio of the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine is preferably 1: 4 to 4: 1, more preferably 2: 1 to 4: 1, especially 3 1: 1 to 4: 1 are preferred. This volume ratio is based on the water-soluble extract components extracted from papaya water-soluble extract components and white flower serpentine grass water-soluble extract components under the same conditions using equal amounts of water from equal amounts of leaves. This is the volume ratio.

  A mixture of both the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine can be taken as it is and used as a medicine for preventing, treating or improving cancer. Moreover, this mixture can also be mixed with the appropriate component normally used for drinks, and can be taken as a drink. Alternatively, the mixture may be dried as necessary, and then taken in the form of a powder, a tablet or the like by an ordinary method in addition to excipients ordinarily used for powders, tablets or the like.

  A mixture of the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine can also be used for health food, functional food, food for specified health use, nutritional supplement, enteral nutrition food, and the like. To a mixture of both the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine, a commonly used compound, for example, a stabilizer, a preservative, a colorant, a flavor, a vitamin, etc. It can be processed into drinks, powders, liquids, tablets, powders, etc. by ordinary methods and used as health foods, functional foods, foods for specified health use, dietary supplements, enteral nutritional foods, etc. .

  The composition of the present invention comprising both the water-soluble extract component of papaya and the water-soluble extract component of white flower serpentine is particularly effective for preventing, treating or improving solid cancer. For example, in lung large cell cancer, lung adenocarcinoma, lung cancer, breast cancer, cervical cancer, lung mesothelial cancer, pancreatic cancer and the like. The dosage for the prevention, treatment or improvement of these cancers is a mixture of a water-soluble extract of papaya and a water-soluble extract of white serpentine, usually from 100 to 750 ml per day for 1 to 3 months. It is preferable to take it daily. Similarly, when edible as health foods, functional foods, foods for specified health use, dietary supplements, enteral nutritional foods, etc., water-soluble extract components of papaya and white flower snake tongue are also included. It is preferred that the mixture be eaten daily in an amount of usually 100 ml to 750 ml per day for 1 to 3 months.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited to these Examples and Test Examples.
Example 1
Preparation of water-soluble extract from leaves of papaya and white flower serpentine grass The water-soluble extract from the leaves of papaya and white flower serpentine grass was prepared by the following method.
Step 1: 20 g of tea leaves obtained by drying the leaves of papaya and white flower serpentine were placed in 400 ml of water and boiled for 1 hour.
Step 2: The temperature was kept at 60-70 ° C. and concentrated to about 100 ml over about 3 hours.
Step 3: After filtering through sterilized gauze to remove large tea leaves, the solution was concentrated to about 50 ml at 60-70 ° C.
Step 4: Centrifugation was performed at 9000 rpm for 20 minutes in a high-speed centrifuge, and the supernatant was collected.
Step 5: Sequentially passed through a sterile filtration filter having a pore size of 0.8, 0.45, and 0.22 · m to prepare a final liquid volume of 20 ml.
Step 6: 1 ml of each extract was dispensed into a sterilized 1.5 ml tube and stored at 4 ° C.
In order to prevent endotoxin contamination throughout the process, all disposable sterilization instruments were used. In addition, a 20 ml concentrated solution derived from 20 g of tea leaves was used as a standard concentration for the following studies.

Test example 1
1. Isolation of human peripheral blood mononuclear cells and measurement of cytokine production Method Fresh peripheral blood derived from healthy subjects was separated by specific gravity separation method using Lymphoprep ^™ . PBMC (2 × 10 ⁵ / well) was seeded on a 96-well flat bottom plate on which an anti-human CD3 antibody (OKT3, 1 μg / ml) was immobilized. Furthermore, solubilized anti-human CD28 antibody (4B10) was added at a final concentration of 5 μg / ml. As the culture solution, RPMI 1640 (Sigma) containing 10% FCS (Biowest) was used. The tea leaf extract was diluted in the range of 200 to 800 times the final concentration and added to the medium.
The supernatant after 24 hours of culture was collected, and cytokine production was measured by ELISA (OptEIA ^™ , BD). The cell viability after culture was analyzed by flow cytometry after double-staining the collected cells with FITC-Annexin V (BD) and 7-AAD (Promega).

2. result
Immunoregulatory effect of extracts on peripheral blood-derived lymphocytes (1) Presence or absence of induction of cell death in healthy human PBMC In order to exclude direct cytotoxicity of tea leaf-derived components, cell viability after culture was examined. . The results are shown in FIG. In FIG. D. Is a water-soluble extract component from the leaves of white flower serpentine grass, C.I. P. Refers to a water-soluble extractable component from papaya leaves. Hereinafter, this abbreviation may be used in the present specification and drawings.
Annexin V positive cells at the point of addition of 200-fold to 800-fold extract do not add 65.4%, and added cells do not drop 65%. If this concentration is within 24 hours, the survival rate is directly affected. This condition was determined in the subsequent analysis because it was considered that there was no effect.

(2) Analysis of cytokine production IL-2 and IL-4 production decreased in a concentration-dependent manner from the 800-fold point, and was suppressed by 70% or more at 200-fold (FIG. 2). Although the phenomenon of cell death has not yet occurred due to the decrease in the production of these cell growth factors, it is very early for activation via the CD3 / T cell receptor (TCR) complex in PBMC. It is possible that suppression was induced from this stage.
IL-12p40 showed the most enhanced production at the 800-fold point for both extracts, and thereafter decreased in a concentration-dependent manner (FIG. 3). IL-12p70 is similar, but C.I. P. Than H. D. The addition has a slightly higher induction ability. The same tendency was observed for IFN-γ, TNF-α, and IL-10 (FIG. 4). Thereby, it was considered that some substance derived from the extracted component may have an immunostimulatory effect to enhance cell function even though it does not contribute to the enhancement of proliferation. These results are shown in H.C. D. And C.I. P. Both extracts were observed either alone or in a mixed state.

Test example 2
Measurement of human tumor cell line culture and proliferation ability Methods As solid tumor lines, Lu99 (Lung large cell carcinoma), A549, PC14 (lung adenocarcinoma) HepG2, Huh-7 (liver cancer), MCF-7 (breast cancer), Hela (cervical cancer), H2452, JMN, MESO-4 (pulmonary mesothelioma), CaPan1, Panc1 (pancreatic cancer) were used.
Each cell line was cultured in RPMI 1640 containing 10% FCS or DMEM (Sigma).
Each tumor cell line was seeded on a 96-well flat-bottom plate at 1 × 10 ⁴ / Well, and adherent cells were sufficiently attached to the bottom surface. Thereafter, an extract diluted 50 to 400 times was added and cultured for 24 hours. The proliferation ability was measured by labeling with [ ³ H] -thymidine for 18 hours until the end of the culture to measure the radioactivity.

2. Results Regarding A549 and Lu99, [ ³ H] -thymidine incorporation was low, and no significant results were obtained. Therefore, this analysis method was considered unsuitable. On the other hand, other cell lines with high uptake (cervical cancer, breast cancer, lung adenocarcinoma, pulmonary mesothelioma, liver cancer, pancreatic cancer) all inhibit cell growth at a high concentration (50 times). Of course, H.P. is used up to 400 times the concentration. D. Or C.I. P. In both cases, a growth inhibitory effect was observed (FIG. 5). In any cell line, H. D. Than C. P. The suppression effect tended to be higher.
Addition of the mixture showed growth inhibition as well as addition of a single substance. When the single addition and the mixed addition at several ratios were compared at the highest dilution point of 400 times, by mixing, cervical cancer (FIG. 6A), breast cancer (FIG. 6B), liver cancer (FIG. 6C) ), Lung adenocarcinoma (FIG. 6D), lung mesothelioma (FIG. 6E), and pancreatic cancer (FIG. 6F), synergistically antitumor effects were observed. Regarding pancreatic cancer, the same tendency was observed for another cell line called Panc-1, but to mention the presence or absence of synergistic effect, the uptake of [ ³ H] -Thymidine itself compared to other cell lines. Low (FIG. 5), judged not appropriate.
The synergistic effect of mixing is as follows. D. : C.I. P. The highest ratio was observed when used at a ratio of 1: 3-1: 4.

1. Regarding the immunostimulatory action of the extract, the point of 800-fold to 400-fold dilution where suppression of the growth factor of activated PBMC and enhancement of cell function were observed was considered to be a concentration slightly below the threshold value for the development of cytotoxicity. Exceeding that concentration will likely cause direct cell damage. However, these extracts are based on ingestion and cannot be present in the blood at physiologically high concentrations, so the results obtained here are consistent and very interesting. It is.
P40, which is a subunit of IL-12p70, is a cytokine produced mainly by antigen-presenting cells (APC), and it is known that production is dramatically enhanced by the activation of APC (Brombacher F, Kastelein RA, Alber G. Novel il-12 family members shed light on the orchestration of th1 responses. Trends Immunol 2003; 24 (4): 207-12). On the other hand, p35 is reported to be produced from a wide range of lymphocytes (Watford WT, Moriguchi M, Morinobu A, O'Shea JJ. The biology of il-12: Coordinating innate and adaptive immune responses. Cytokine Growth Factor Rev 2003; 14 (5): 361-8), CD40L-deficient mice report that stimulation via CD40-40L is essential for these subunits to form dimers and function as p70. (Cella M, Scheidegger D, Palmer-Lehmann K, Lane P, Lanzavecchia A, Alber G. Ligation of cd40 on dendritic cells triggers production of high levels of interleukin-12 and enhances t cell stimulatory capacity: Tt help via apc activation. J Exp Med 1996; 184 (2): 747-52).
However, the extract-derived component in the “unpurified” state includes various naturally-derived substances, and the possibility of being recognized as a mere “foreign substance” in the innate immune response is difficult to discard. As a result of stimulating APC directly as “foreign matter”, it is considered that the production of p40 is increased, and in order to eliminate the possibility, sufficient consideration was given to the purification method or the dilution factor. As a result, p40 production and p70 production are correlated and increased, and may be involved not only in the activation of APC but also in the modification of the acquired immune response between T-APC and T-B cells. Shown here. In addition, inflammatory cytokines such as TNF-α and IFN-γ are important factors that modify the subsequent immune response. In particular, I would like to expect the antitumor effects of these cytokines. Further, since IFN-γ and TNF-α are induced in correlation with IL-12p70, the involvement of substances having Th1 inducibility could be predicted.

2. Regarding the anti-tumor effect of the extract, the growth suppression observed in solid tumor cell lines was judged to be due to the induction of cell death by microscopic examination, although there was no appropriate analysis method. And when these cell lines were used, as shown in FIG. 8, the synergistic effect by a liquid mixture was recognized, and the possibility that it might be enhanced by mixing some factor which induces cell damage was considered.
In the case of adherent cells, it was essential to add the extract after completely adhering to prevent the extract from directly inhibiting the adhesion to the bottom of the culture plate. The adhesion-inhibiting effect of the extract itself also contributes to the anti-tumor effect that suppresses cell-to-cell or cell-to-extracellular matrix adhesion and suppresses tumor growth and metastasis expected in vivo. The possibility of doing was also considered.

3. Summary According to this test example, D. And C.I. It was clearly shown that P alone has a cell growth inhibitory effect and an immunostimulatory effect, and that the effect functions synergistically without being offset when mixed. In the analysis of this test example, the usefulness of mixing two liquids was confirmed for the cytotoxic activity against solid tumor cell lines.

PBMCs were seeded at a concentration of 2 × 10 ⁵ cells / well in a 96-well flat-bottom plate on which an anti-CD3 antibody (1 μg / ml) had been immobilized, and solubilized anti-CD28 antibody (5 μg / ml) was added. H. at the start of culture. D. , C.I. P. Alone or H.C. D. + C. P. (HD: C.P. = 1: 1, 1: 4, 4: 1) was added. Cells were collected 24 hours later, double-stained with FITC-annexin V and 7-AAD, and analyzed by flow cytometry. The X axis shows the dilution concentration of each extract (no addition and 800 to 200 times), and the Y axis shows the percentage of FITC-annexin V positive cells. H. D. Or C.I. P. Alternatively, cytokines in the supernatant in the presence of the mixture were examined by ELISA for (A) IL-2 and (B) IL-4. The X-axis shows the dilution concentration of each extract (no addition and 800-fold to 200-fold dilution), and the Y-axis shows the cytokine concentration. Data showed results for two representative donors. H. D. Or C.I. P. Alternatively, the cytokines in the supernatant in the presence of the mixed solution were examined by ELISA for (A) IL-12p40 and (B) IL-12p70. The upper data shown in A is unstimulated PBMC, and the lower data is in the presence of CD3 + CD28 stimulation and the extract is added. The X-axis shows the dilution concentration of each extract (no addition and 800-fold to 200-fold dilution), and the Y-axis shows the cytokine concentration. Data showed results for two representative donors. H. D. Or C.I. P. Alternatively, cytokines in the supernatant in the presence of the mixed solution were examined by ELISA for (A) IFN-γ and (B) TNF-α (C) IL-10. The upper data shown in B and C is unstimulated PBMC, and the lower data is in the presence of CD3 + CD28 stimulation and the extract is added. The X axis shows the dilution concentration of each extract (no addition and 800 to 200 times dilution), and the Y axis shows the cytokine concentration. Data showed results for two representative donors. H. D. Or C.I. P. The uptake of ³ [H] -thymidine after 24-hour culture of a tissue-derived tumor cell line in the presence of the extract was examined. The X axis shows the dilution concentration of each extract (no addition and 400 to 50 times dilution), and the Y axis shows the radioactivity. Data showed the result about a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. A tumor cell line of cervical cancer (Hela) was used. The data showed the results for a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. A breast cancer (MCF-7) tumor cell line was used. The data showed the results for a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. Liver cancer (HepG2, Huh-7) tumor cell lines were used. The data showed the results for a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. A tumor cell line of lung adenocarcinoma (PC-14) was used. The data showed the results for a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. A lung mesothelioma (JMN, H2452) tumor cell line was used. The data showed the results for a representative example from three experiments. H. D. Or C.I. P. ³ [H] -thymidine uptake after 24 hours of culture of tissue-derived tumor cell lines in the presence of 400-fold diluted extract was compared with no addition, single addition, and mixed addition. The numerical values in the graph indicate radioactivity. A tumor cell line of pancreatic cancer (CaPan-1) was used. The data showed the results for a representative example from three experiments.

Claims (9)

  A composition for preventing, treating or ameliorating cancer, comprising a water-soluble extract component of papaya (Carica papaya) and a water-soluble extract component of Hedyotis Diffusa as active ingredients.   The composition according to claim 1, wherein the water-soluble extractive component of papaya is a component extracted by roasting papaya leaves with hot water.   The composition according to claim 1 or 2, wherein the water-soluble extractive component of white flower serpentine is a component extracted by decocting the leaves of white flower serpentine with boiling water.   The composition according to any one of claims 1 to 3, for preventing, treating or ameliorating solid cancer.   The composition according to claim 4, wherein the solid cancer is lung large cell cancer, lung adenocarcinoma, lung cancer, breast cancer, cervical cancer, lung mesothelioma or pancreatic cancer.   The composition according to any one of claims 1 to 5, which is a pharmaceutical composition.   The composition according to any one of claims 1 to 5, which is a food composition.   The composition according to claim 7, which is a health food, a functional food, a food for specified health use, a dietary supplement or an enteral nutrition food.   9. A composition according to claim 7 or 8 in the form of a drink, powder or tablet.