JP2008212020A - Device and method for decomposing living body tissue - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a device and method for decomposing a living body tissue, capable of recognizing the progressing state of the decomposition at an early period, regardless of the individual differences of the living body tissues and efficiently obtaining ample number of healthy cellular groups. <P>SOLUTION: This device 1 for decomposing the living body tissue is equipped with: a container 2 for storing a digestive liquid B containing a digestive enzyme for decomposing the living body tissue A by forming a liquid surface B<SB>1</SB>; a supporting member 3, arranged capable of lifting and lowering vertically in the container 2 and supporting the living body tissue A which is to be decomposed in the vertical direction; a vertical lifting and lowering mechanism 4 for lifting and lowering the supporting member 3; a measuring part 5, arranged in between the lifting and lowering mechanism 4 and supporting member 3 and measuring the force acting on the supporting member 3 in a perpendicular direction; and a decomposition state-determining part 6 for determining the progressing state of decomposition of the living body tissue, based on the change of the force measured by the measuring part 5 during the process of the lifting up and down of the supporting member 3 by the lifting and lowering mechanism 4. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a biological tissue decomposing apparatus and a biological tissue decomposing method for decomposing a biological tissue such as adipose tissue to obtain a group of cells such as adipose-derived stem cells.

Conventionally, a technique for separating adipose-derived cells contained in adipose tissue by collecting human adipose tissue and stirring it with digestive enzymes and physiological saline is known (see, for example, Patent Document 1). .
In this technique, digestion progresses with agitation, adipose tissue is decomposed, and adipose-derived cell groups are separated, and the end of digestion is determined by counting the time required for digestion.

International Publication No. 2005/012480 Pamphlet

  However, since digestion of adipose tissue is greatly different depending on individual differences, there is a problem in that a fat-derived cell group having a certain quality cannot be obtained when the end of digestion of adipose tissue is determined based on elapsed time. That is, if the digestion time is too short, a sufficient amount of cell groups cannot be obtained, and if the digestion time is too long, the soundness of the cell groups is impaired by the action of the enzyme. In addition, in order to obtain sufficient adipose-derived cell groups regardless of individual differences in adipose tissue, it is necessary to perform digestion for a long time in accordance with the treatment time required for adipose tissue that is slowly digested, thus shortening the treatment time. There is an inconvenience that can not be done.

  The present invention has been made in view of the above-described circumstances, and provides a biological tissue decomposing apparatus and a biological tissue decomposing method that make it possible to obtain a cell group of constant quality regardless of individual differences in adipose tissue. It is an object.

In order to achieve the above object, the present invention provides the following means.
The present invention provides a container for storing digestive fluid containing digestive enzymes that decompose biological tissue in a liquid surface, and a support that is disposed in the container so as to be movable up and down and supports the biological tissue to be decomposed in the vertical direction. A member, an elevating mechanism for elevating and lowering the support member, a measuring unit disposed between the elevating mechanism and the support member and measuring a vertical force acting on the support member, and the support by the elevating mechanism There is provided a biological tissue decomposing apparatus including a decomposition status determining unit that determines a progress status of decomposition of a biological tissue based on a change in force measured by the measuring unit in the process of raising and lowering a member.

  According to the present invention, when a living tissue is put into the digestive fluid stored in the container and stirred, the biological tissue is decomposed by the digestive enzyme contained in the digestive fluid, and the cells are taken out. The removed cells are separated from the living tissue and float in the digestive juice to become a cell suspension. Therefore, the volume of the living tissue is reduced as the decomposition of the living tissue progresses.

  By measuring the force acting on the support member with the measuring unit while raising and lowering the support member that supports the living tissue in the container by the operation of the lifting mechanism, the relationship between the living tissue and the supporting member, The magnitude of the force measured from the relationship with the digestive fluid level changes.

  For example, when the living tissue is an adipose tissue, the adipose tissue floats near the liquid surface of the digestive juice. Therefore, when the adipose tissue is not in contact with the support member, the force acting on the support member is the weight of the support member. If only a part of the support member is exposed from the liquid surface of the digestive juice during the ascending process, the buoyancy is weakened correspondingly, and the measured value of the force measured by the measuring unit increases.

  After the adipose tissue comes into contact with the support member, the support member's own weight, buoyancy, and the weight and buoyancy of the adipose tissue act on the support member. Therefore, when a part of the adipose tissue is exposed from the liquid surface of the digestive fluid during the ascent process, the buoyancy is weakened accordingly, and the measured value of the force measured by the measurement unit increases.

  Thus, in the process of raising or lowering the support member, the value of the force measured by the measurement unit changes depending on the living tissue from the liquid level of the digestive juice or the exposure amount of the support member, and the manner of the change is also Change. Therefore, by detecting the change, it is possible to detect where the living tissue is arranged with respect to the liquid level of the digestive juice, and to calculate the volume of the remaining living tissue without being decomposed.

  As a result, the decomposition state determination unit determines the progress of decomposition of the living tissue based on the change in force measured by the measurement unit during the lifting / lowering process of the support member by the lifting mechanism, thereby making a comparison necessary for the end of the decomposition. Without waiting for an elapse of a long time, it can be determined whether the decomposition has started or whether the decomposition has proceeded normally. Therefore, it is possible to eliminate the waste of time without being decomposed and to decompose the living tissue while preventing degradation of the soundness of the cell group due to being immersed in the digestive fluid for a long time. A group of cells can be obtained.

In the above-described invention, a position detection unit that detects the position of the support member is provided, and the disassembly state determination unit is based on the position of the support member and the measurement unit at the time when the rate of change in force measured by the measurement unit changes. The volume of the living tissue may be calculated based on the measured force, and the progress of decomposition of the living tissue may be determined based on the calculated volume.
By doing in this way, it can be judged that the time when the rate of change of the force measured by the measuring unit changes is the time when the living tissue starts to be exposed from the liquid level of the digestive fluid, and the position of the support member detected by the position detecting unit Based on the above, it is possible to easily grasp the volume of the living tissue remaining in the digestive juice without being decomposed.

  Further, the present invention is a container for storing a digestive fluid containing a digestive enzyme that decomposes a biological tissue, and a support member that is disposed in a state immersed in the digestive fluid of the container and suppresses the floating of the biological tissue in the digestive fluid. A biological tissue decomposition comprising: a measurement unit that measures a vertical force acting on the support member; and a decomposition state determination unit that determines a progress of the decomposition of the biological tissue based on the force measured by the measurement unit Providing the device.

  According to the present invention, when decomposing a living tissue that floats in the digestive juice, the supporting member is restrained from rising by the supporting member that is placed in a state immersed in the digestive juice. The dead weight and buoyancy, and the weight and buoyancy of the living tissue act in the vertical direction. As the decomposition of the living tissue progresses, the volume of the living tissue decreases, so that the weight and buoyancy of the living tissue decrease. Therefore, by measuring these resultant forces by the measuring unit, it is possible to measure a decrease in the volume of the living tissue, and the decomposition state determining unit can easily determine the progress of decomposition. As a result, the living tissue can be decomposed so that a cell group of a certain quality can be obtained.

  The present invention also includes a decomposition step in which the biological tissue is introduced into digestive fluid containing a digestive enzyme that decomposes the biological tissue and the decomposition proceeds, and a support member disposed below the biological tissue is pulled up during the decomposition step. There is provided a biological tissue decomposition method including a measurement step of measuring the weight and a determination step of determining the progress of decomposition of the biological tissue based on a change in the weight measured by the measurement step.

  According to the present invention, the measurement step performed in the middle of the decomposition step for decomposing the living tissue in the digestive juice measures the weight while pulling up the support member from below the living tissue, and determines based on the change in the weight Since the step determines the progress of decomposition, the volume of the living tissue remaining in the digestive juice can be confirmed when the living tissue is exposed from the liquid level of the digestive juice. Therefore, it is possible to determine the progress status easily and quickly without completely extracting the living tissue from the digestive juice.

In the above invention, the measurement step measures the weight of the support member in association with the position of the support member, and the determination step determines the support member at the time when the rate of change of the weight with respect to the position changes. The volume of the living tissue may be calculated based on the position and the weight, and the progress of decomposition of the living tissue may be determined based on the calculated volume.
In this way, the time when the rate of change of the weight with respect to the position changes can be determined as the time when the living tissue starts to be exposed from the liquid level of the digestive fluid, and it is not decomposed based on the detected position of the support member. The volume of living tissue remaining in the digestive juice can be easily grasped.

  The present invention also includes a decomposition step in which the biological tissue is introduced into digestive fluid containing a digestive enzyme that decomposes the biological tissue and the decomposition proceeds, and a supporting member disposed above the biological tissue suppresses the floating of the biological tissue. There is provided a biological tissue decomposition method including a measurement step for measuring weight, and a determination step for determining a progress state of decomposition of the biological tissue based on the weight measured by the measurement step.

  According to the present invention, when decomposing a living tissue that floats in the digestive juice, the supporting member is restrained from being lifted by the supporting member placed in the digestive juice so that The dead weight and buoyancy, and the weight and buoyancy of the living tissue act in the vertical direction. As the decomposition of the living tissue progresses, the volume of the living tissue decreases, so that the weight and buoyancy of the living tissue decrease. Therefore, by measuring these resultant forces by the measuring unit, it is possible to measure a decrease in the volume of the living tissue, and the decomposition state determining unit can easily determine the progress of decomposition. As a result, the living tissue can be decomposed so that a cell group of a certain quality can be obtained.

  According to the present invention, the progress of degradation can be confirmed at an early stage regardless of individual differences in living tissue, and a sufficient number of healthy cell groups can be efficiently obtained.

A biological tissue decomposing apparatus 1 according to a first embodiment of the present invention will be described below with reference to FIGS. 1 and 2.
The biological tissue decomposing apparatus 1 according to the present embodiment is an apparatus for decomposing adipose tissue A. As shown in FIG. 1, a proteolytic enzyme (digestive enzyme) such as trypsin that decomposes adipose tissue A and physiological saline. A first container 2 for storing a digestive juice B obtained by mixing water, a solvent such as Lactated Ringer's solution or a buffer solution, and a second container (supporting member) disposed in the first container 2 so as to be movable up and down ) 3, a lifting mechanism 4 that lifts and lowers the second container 3, and a measurement that measures the vertical force acting on the second container 3 that is disposed between the lifting mechanism 4 and the second container 3. An apparatus (measurement unit) 5 and a control unit 6 that determines the progress of decomposition based on the measurement result are provided.

The first container 2 is opened to the atmosphere the upper opening 2a, it is made to form a free liquid surface B ₁ so as to store the digestive juices B.
Said 2nd container 3 has the accommodating part S which accommodates the adipose tissue A, The magnitude | size which passes the digestive liquid B and the decomposed cell group on the upper surface and the lower surface, and prohibits the passage of the adipose tissue A. A filter 7 having a through hole (not shown) is arranged. Thereby, when the 2nd container 3 is raised / lowered in the 1st container 2, the fat tissue A is hold | maintained in the accommodating part S in the accommodating part S. As shown in FIG. Further, when the second container 3 is moved up and down in the first container 2, since the digestive juice B passes through the filter 7, the digestion liquid B hinders the lifting and lowering operation of the second container 3. There is no such thing.

The elevating mechanism 4 is, for example, a linear moving mechanism including a slider 9 that is moved up and down by a motor 8. The position of the slider 9 is detected by an arbitrary position detection device (not shown) such as an encoder of the motor 8.
The measuring device 5 is a weighing scale that is fixed to the slider 9 and suspends the second container 3.

A biological tissue decomposing method using the fabric tissue decomposing apparatus 1 according to this embodiment configured as described above will be described below.
In the biological tissue decomposition method according to this embodiment, first, the volume of the adipose tissue A before the start of digestion is measured and stored. Then, as shown in FIG. 2, the biological tissue A is accommodated in the second container 3 (step S1), and the biological tissue A is accommodated in the digestive fluid B stored in the first container 2. The second container 3 is immersed (step S2).

  Then, the adipose tissue A is digested while stirring the digested liquid B in the first container 2 for a predetermined time (step S3), and the progress of decomposition is measured in the process (step S4). In the process of measuring the progress of degradation, the progress of degradation is confirmed by calculating the rate of decrease in the volume of adipose tissue A remaining in the digestive juice B. Then, it is determined whether or not the volume reduction rate has exceeded a predetermined threshold value (step S5). If it exceeds, the digestion process of the adipose tissue A is terminated. repeat.

Here, the process of measuring the progress of decomposition will be described with reference to FIGS.
In order to measure the progress, first, the second container 3 containing the adipose tissue A in the containing part S is raised at a constant speed in the digestive fluid B of the first container 2 by operating the lifting mechanism 4. (Step S41). Thus, as shown in FIG. 4, the positional relationship between the liquid level B ₁ and adipose tissue A digestion solution B is changed.

Figure 4 (a) is a first state of being immersed in the liquid level B ₁ below the digestive fluid B to the upper surface of the second container 3. At this time, the adipose tissue A in the second container 3 floats in the housing part S, and is disposed at a position in close contact with the filter 7 on the upper surface, which is the upper limit of the housing part S. That is, the second container 3 has its own weight W, the buoyancy w of the second container 3, the weight WA of the fat tissue A, and the buoyancy wa of the fat tissue A.
F = (W−w) + (WA−wa)
The force F acts. Then, the force F, as shown in FIG. 5, as long as the upper surface of the second container 3 is maintained in the first state under the liquid surface B ₁ of the digestive juices B, the second container 3 It will not change even if it is raised or lowered.

FIG. 4 (b) is a second state in which the upper surface of the second container 3 is exposed on the liquid surface B ₁ of the digestive juices B. At this time, adipose tissue A, because its upper surface reaches the liquid surface B ₁ of the digestive liquid B, stops at that position in balance and the weight WA and buoyancy wa, only the second container 3 is raised. Therefore, the force F acting on the second container 3 is
F = (W−w)
It becomes. Buoyancy w varies according to the exposure amount to the second digestion solution B of the container 3 of the liquid level B ₁ above. Therefore, in the second state, as the second container 3 is raised, the buoyancy w decreases, and the force F acting on the second container 3 increases monotonously as shown in FIG.

FIG. 4 (c), the lower surface of the second container 3, shows a time (third state) in contact with the adipose tissue A has emerged on the liquid surface B ₁ position of digestive juices B.
Then, FIG. 4 (d), adipose tissue A in contact with the lower surface of the second container 3, begins to rise with increasing second container 3, the upper part of the liquid surface B ₁ of the digested slurry B Shows a fourth state exposed.

At this time, similarly to the first state, the following force F acts on the second container 3 again.
F = (W−w) + (WA−wa)
However, unlike the first state, the buoyancy of the adipose tissue A decreases, the weight increases, and the force F increases. Therefore, as shown in FIG. 5, the slope value, which is the rate of change of the force F with respect to the position of the second container 3, changes between the second state and the fourth state across the third state. To do.

That is, the force F acting on the second container 3 is measured by the measuring device 5 while detecting the position of the slider 9 by the position detecting device while raising the second container 3 at a constant speed (step S41). The third state in which the rate of change of the force F with respect to the position changes is detected (step S42). Thus, it is possible to detect the time when the adipose tissue A begins exposed above the liquid surface B ₁ of the digestive juices B. Specifically, by determining whether or not the rate of change has exceeded a predetermined threshold value (step S43), if it has exceeded, the time point is specified and the process proceeds to the next step S43. Then, the ascending operation of the second container 3 is continued (step S41).

Then, the liquid surface B ₁ position, the position of the lower surface of the second container 3 and the crossing of the second container 3 when the rate of change of the force F with respect to the position of the second container 3 exceeds a predetermined threshold value. The volume of the adipose tissue A is calculated using the area (step S44). Thereafter, using the stored volume of adipose tissue A before the start of digestion, the volume reduction rate at the time of measurement can be easily calculated (step S45), and the progress of decomposition can be confirmed. it can.

  Then, it is determined whether or not the time change of the volume decrease rate is smaller than a predetermined threshold value (step S46). If the volume has not decreased, an alarm is issued (step S47), and the volume decreases. If so, the digestion process continues.

  Thus, according to the biological tissue decomposing apparatus 1 and the biological tissue decomposing method according to the present embodiment, in the middle of the digestion process without waiting for sufficient time for the adipose tissue A to be completely decomposed. By performing the above measurement, the progress of decomposition can be easily confirmed. Therefore, when the disassembly is not performed for some reason, it is possible to confirm the fact at an early stage by notifying this and deal with it. Thereby, it is possible to prevent the occurrence of waste that a long time elapses without being disassembled.

  Moreover, according to the biological tissue decomposing apparatus 1 and the biological tissue decomposing method according to the present embodiment, the adipose tissue A remaining in the digestive fluid B is not completely lifted from the digestive fluid B, but most of the digestive fluid B is concentrated. The progress of decomposition can be confirmed in the state of being immersed. Therefore, the progress status can be confirmed without interrupting the digestion process, and the digestion process can be made more efficient.

  In this case, when the adipose tissue A is pulled up from the digestive juice B and the weight or volume thereof is measured, the adipose tissue A is pulled up from the digestive fluid B and waits until the digestive fluid B comes out of the adipose tissue A. Although much time is required to re-inject the adipose tissue A into the digestive juice B after the measurement, according to the present embodiment, the progress of decomposition can be confirmed easily and quickly.

  Furthermore, according to the biological tissue decomposing apparatus 1 and the biological tissue decomposing method according to the present embodiment, the progress of the decomposition of the adipose tissue A can be easily and quickly confirmed, and the adipose tissue A can be obtained by performing a confirmation operation multiple times. It is possible to estimate the end of the decomposition of. Then, the progress of decomposition is confirmed again at the estimated time, and when it is determined that the volume of the adipose tissue A has decreased to a predetermined decrease rate, the digestion process can be immediately terminated. Therefore, it is possible to prevent the occurrence of inconvenience that the soundness is lowered by the proteolytic enzyme when the degraded fat-derived cell group is continuously immersed in the digestive fluid for a long time.

In the biological tissue decomposing apparatus 1 and the biological tissue decomposing method according to this embodiment, the volume of the adipose tissue A before the start of the decomposition is stored, and the progress of the decomposition is calculated by calculating the volume reduction rate relative thereto. Although the situation has been confirmed, the following method may be adopted instead.
For example, when the decomposition of the adipose tissue A is started, the above measurement is performed, and the position of the slider 9 in the third state at that time is stored. Then, in the middle of the subsequent digestion process, the progress of the decomposition of the adipose tissue A can be easily determined by comparing with the position of the slider 9 in the third state detected by performing the measurement again. Can do.

  Further, in the present embodiment, the second container for containing the adipose tissue A has been described as an example. However, instead of this, if the adipose tissue A can be supported in the vertical direction so as not to fall off, a plate shape is used. The thing of a thing and a bag shape may be sufficient.

Next, a biological tissue decomposing apparatus 10 and a biological tissue decomposing method according to the second embodiment of the present invention will be described below with reference to FIGS. 6 and 7.
As shown in FIG. 6, the biological tissue decomposing apparatus 10 according to this embodiment includes a first container 11 that stores digestive fluid B containing a digestive enzyme that decomposes adipose tissue A, and the first container 11. A second container 12 that is disposed so as to be immersed in the digestive juice B and suppresses the floating of the adipose tissue A in the digestive juice B, and a measuring device that measures the vertical force acting on the second container 12 13 and a control unit 14 that determines the progress of the decomposition of the adipose tissue A based on the force measured by the measuring device 13. In the figure, reference numeral 15 is a support base for supporting the measuring device 13, and reference numeral 16 has a through-hole (not shown) having a size that allows the digestive juice B and the decomposed cell group to pass therethrough and prohibits passage of the adipose tissue A. It is a filter.

In a state where the adipose tissue A is not accommodated in the second container 12, the force F measured by the measuring device 13 uses the own weight W of the second container 12 and the buoyancy w of the second container 12. ,
F = W−w
It is expressed.

In a state where the adipose tissue A is injected into the second container 12, the adipose tissue A floats in the digestive fluid B, and always generates a force that pushes the second container 12 upward. When the weight of the fat tissue A is WA and the buoyancy of the fat tissue A is wa, the force F measured by the measuring device 13 is
F = (W−w) + (WA−wa)
It becomes. Here, since the 2nd container 12 is maintained in the state immersed in the digestive liquid B, the 1st term (W-w) does not change, and the 2nd term (WA-) with progress of decomposition | disassembly. wa) changes.

FIG. 7 shows the time change of the force F measured by the measuring device 13.
According to FIG. 7, when the adipose tissue A is injected into the second container 12, the force F for pulling up the second container 12 at time t <b> 1 is reduced by the buoyancy wa of the adipose tissue A. In the subsequent digestion process, the buoyancy wa is weakened as the volume of the adipose tissue A decreases, so that the force for pulling up the second container 12 increases.

At time t2, since the fat tissue A is sufficiently decomposed, the force F for pulling up the second container 12 becomes substantially equal to the state before the fat tissue A is injected into the second container. The control unit 14 can end the digestion process at this point.
As described above, according to the biological tissue decomposing apparatus 10 and the biological tissue decomposing method according to the present embodiment, the adipose tissue A is decomposed while being immersed in the digestive fluid B using the buoyancy wa of the adipose tissue A. It is possible to determine the progress status of. Therefore, similarly to the first embodiment, there is an advantage that a sufficient number of healthy cell groups can be acquired efficiently without interrupting the digestion process without wasting time.

Moreover, according to the biological tissue decomposing apparatus according to the present embodiment, unlike the first embodiment, since the lifting mechanism 4 for pulling up the second container 12 is not necessary, the apparatus can be configured simply and used durablely. There is also an advantage that can be achieved.
In each of the above-described embodiments, the fatty tissue is exemplified and described as the biological tissue A, but instead, it is applied to the decomposition of another biological tissue, in particular, the biological tissue that floats in the digestive juice B. It is good as well.

1 is an overall configuration diagram showing a biological tissue decomposing apparatus according to a first embodiment of the present invention. It is a flowchart which shows the biological tissue decomposition | disassembly method which concerns on the 2nd Embodiment of this invention. FIG. 3 is a flowchart for explaining a progress measurement process of decomposition in the biological tissue decomposition method of FIG. 2. FIG. 4 is an explanatory diagram for explaining a progress measurement process of decomposition in FIG. 3. FIG. 4 is a graph showing the relationship between the force measured in the disassembly progress measurement step of FIG. 3 and the position of the slider. FIG. It is a whole block diagram which shows the biological tissue decomposition | disassembly apparatus which concerns on the 2nd Embodiment of this invention. It is a graph which shows the time change of the force measured in the progress measurement process of decomposition | disassembly in the biological tissue decomposition | disassembly method using the biological tissue decomposition | disassembly apparatus of FIG.

Explanation of symbols

A Adipose tissue (living tissue)
B digestive fluid B ₁ liquid surface S3 decomposition step S4 measurement step S44 volume calculation step S46, S5 determination step 1,10 biological tissue decomposition apparatus 2,11 first container (container)
3,12 Second container (support member)
4 Lifting mechanism 5,13 Measuring device (measuring unit)
6,14 Control unit (disassembly status determination unit)

Claims (6)

A container for storing a digestion solution containing digestive enzymes that decompose biological tissue, forming a liquid surface;
A support member disposed in the container so as to be movable up and down, and supporting a biological tissue to be decomposed in a vertical direction;
An elevating mechanism for elevating the support member;
A measuring unit that is disposed between the lifting mechanism and the support member and measures a vertical force acting on the support member;
A biological tissue decomposing apparatus comprising: a decomposition state determining unit that determines a progress state of decomposition of a biological tissue based on a change in force measured by the measuring unit in the process of moving up and down the support member by the lifting mechanism. A position detector for detecting the position of the support member;
The decomposition state determination unit calculates the volume of the living tissue based on the position of the support member at the time when the change rate of the force measured by the measurement unit changes and the force measured by the measurement unit. The biological tissue decomposing apparatus according to claim 1, wherein the progress of the decomposition of the biological tissue is determined based on the volume. A container for storing a digestive juice containing digestive enzymes that decompose biological tissue;
A support member that is placed in a state of being immersed in the digestive fluid of the container, and that suppresses the floating of biological tissue in the digestive fluid;
A measurement unit for measuring a vertical force acting on the support member;
A biological tissue decomposition apparatus comprising: a decomposition state determination unit that determines a progress state of decomposition of a biological tissue based on a force measured by the measurement unit. A decomposition step in which the biological tissue is introduced into a digestive fluid containing a digestive enzyme that decomposes the biological tissue and the decomposition proceeds;
In the middle of the disassembling step, a measuring step for measuring the weight while pulling up the supporting member disposed below the living tissue,
A biological tissue decomposition method including a determination step of determining a progress state of the decomposition of the biological tissue based on a change in weight measured by the measurement step. The measuring step measures the weight of the support member in association with the position of the support member;
The determination step calculates the volume of the living tissue based on the position and weight of the support member at the time when the rate of change of the weight with respect to the position changes, and the progress of the decomposition of the living tissue based on the calculated volume The biological tissue decomposition | disassembly method of Claim 4 which determines. A decomposition step in which the biological tissue is introduced into a digestive fluid containing a digestive enzyme that decomposes the biological tissue and the decomposition proceeds;
A measuring step for measuring the weight of the living tissue while suppressing the floating of the living tissue by a support member disposed above the living tissue;
A biological tissue decomposition method including a determination step of determining a progress state of biological tissue decomposition based on the weight measured in the measurement step.

Images