JP2008131956A - Method for artificial culture of lyophyllum shimeji - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a Lyophyllum shimeji strain having excellent fruit body-forming activity on a culture base using an inexpensive medium material and commercially and artificially cultured, by which the necessary period after transferred to a generation chamber can be shortened. <P>SOLUTION: The Lyophyllum shimeji strain is a mutant strain of strains selected from the group consisting of Lyophyllum shimeji La 01-20 (FERM P-16841), Lyophyllum shimeji La 01-27 (FERM P-17455), and Lyophyllum shimeji La 01-45 (FERM P-17457), and has excellent fruit body-forming capability on a culture base containing corn grains and saw dust of broadleaf trees at a dry weight ratio of 2:1 relative to a culture base containing steamed and pressed barley and saw dust of broadleaf trees at a dry weight ratio of 2:1 weight ratio. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a strain of Lyophyllum shimeji and an artificial cultivation method.

  Honshimeji is a mushroom that grows on the ground of the Quercus Forest or Quercus serrata mixed forest in mid-October, and is the highest grade of edible mushrooms in Japan, along with Matsutake. It has been a mushroom.

  In recent years, edible mushrooms such as enokitake mushrooms, oyster mushrooms, sea cucumbers, beech shimeji mushrooms, maitake mushrooms, etc. have been established with an artificial cultivation method that mainly uses a culture medium that is mixed with sawdust, rice bran, bran and other nutrient sources, regardless of the season throughout the year Mushrooms can be harvested stably.

  Honshimeji is also a very delicious mushroom, so establishment of an artificial cultivation method is desired, but the above-mentioned enokitake mushrooms are wood decaying fungi, whereas Honshimeji is a mycorrhizal fungus and artificial cultivation is difficult. It had been.

  Artificial cultivation of this hon-shimeji has also been studied in part. For example, Patent Document 1 discloses a method for artificially cultivating hon-shimeji mushrooms using wheat, and the inventors use wheat in Non-Patent Document 1. We report the development experiment of Honshimeji fruiting body in the existing medium.

  Patent Document 2 discloses a mycorrhizal mycelium culture method using peat moss as a base material and added with starch or the like. Non-Patent Document 2 discloses a method for cultivating mycorrhizal mycelium. We report a fruit body development experiment of hon-shimeji mushroom with added culture medium.

However, in the method of the inventors of Patent Document 1, since the wheat used for the medium is expensive, the medium cost becomes high. Moreover, the yield of the fruiting body produced by the method described in Patent Document 2 is low, and has not yet reached the level of commercial production.
Japanese Patent Publication No. 8-4427 JP-A-6-153695 Bulletin of the Mycological Society of Japan, Vol. 39, pp. 13-20 (1998) Journal of the Mycological Society of Japan, 35, 192-195 (1994)

  In view of the above-described situation, the object of the present invention is a hon-shimeji mushroom that has a good fruiting body forming ability with a culture medium using an inexpensive medium raw material, and further enables commercial artificial cultivation that can reduce the number of days required after the generation chamber is moved. It is to provide a strain.

  In general, mushrooms are strains belonging to the same species, but it is known that the growth of mycelium and the ability to form fruit bodies differ significantly depending on the location where they are collected. It has also been reported in the Mycological Society of Japan, Vol. 39, pp. 13-20 (1998) that Honshimeji is a medium that uses barley and sawdust and the fruiting body generation amount varies greatly depending on the strain. Based on the idea that there must be a strain that is compatible with a medium using inexpensive millet subfamily plants, the present inventors have collected hon-shimeji mushroom strains from various locations, and as a result of earnest examination, We found Honshimeji strains with good fruiting ability in culture media containing legumes, and cultivated these strains in culture media containing millet fruits, using wheat It has been found that the number of days from the transfer to the generation chamber to the harvesting can be shortened compared to the case of cultivation with the culture medium, and the present invention has been completed.

  To outline the present invention, the first invention of the present invention relates to a strain of Honshimeji, characterized in that it is a strain that forms a good fruiting body in a culture medium containing the fruits of the millet family. A second invention of the present invention relates to a method for artificially cultivating hon-shimeji mushroom, wherein the strain of the first invention is used. A third invention of the present invention relates to a method for artificially cultivating hon-shimeji mushroom, wherein the strain of the first invention is cultivated using a culture medium containing the fruits of the millet family.

That is, the present invention is a group consisting of [1] Lyophyllum shimeji La 01-20 (FERM P-16841), Lyophyllum shimeji La 01-27 (FERM P-17455), and Lyophyllum shimeji La 01-45 (FERM P-17457). A hon-shimeji strain which is a mutant of a strain selected from more than 2% by weight of corn nuts and hardwood sawdust in a dry matter weight ratio compared to a culture medium containing oats and hardwood sawdust in a dry matter weight ratio of 2: 1. Honshimeji strain having a good fruiting body forming ability in the culture medium containing,
[2] A liquid inoculum for artificial cultivation of hon-shimeji mushroom obtained by inoculating and culturing the hon-shimeji strain described in [1] above in a liquid medium,
[3] A culture medium for artificial cultivation of hon-shimeji mushroom obtained by inoculating and cultivating the hon-shimeji strain according to [1] above on a solid culture medium containing corn fruit,
[4] A method for artificially cultivating hon-shimeji mushroom characterized in that the strain described in [1] is cultured in a culture medium containing corn nuts, and [5] the strain described in [1] above contains corn nuts. The present invention relates to a method for producing hon-shimeji mushroom characterized by culturing in a culture medium.

  According to the present invention, there is provided a hon-shimeji strain capable of harvesting a good fruiting body in a culture medium using an inexpensive medium raw material in a shorter number of days after moving to a development chamber. Cultivation became possible. In particular, the La01-20 strain, La01-27 strain, and La01-37 strain have a short required time after moving to the generation room and are suitable for commercial artificial cultivation.

The present invention will be specifically described below.
In the present invention, the fruit of the millet family plant refers to an unprocessed millet family fruit or a processed product of the millet family plant. In addition, the fruit of the millet family plant described in the text will be explained. In the present invention, the fruit of the millet family plant refers to a fruit that is scientifically referred to as fruit or cereal. According to the description of the 4th edition of the Iwanami Biology Dictionary, published by Iwanami Shoten in 1996, fruit is a kind of fruit that is dried and adheres closely to seeds after maturation. This is the fruit of a bamboo family plant.

  Examples of the above-mentioned fruits of the millet family include millet, millet, millet, sugar cane, sorghum, sorghum, corn, and the like, but the millet family that can be used in the present invention is limited to these. There is no such thing as long as it is a fruit of a plant belonging to the family Gramineae. The fruit of the millet family used in the present invention may be fresh or dried. Two or more kinds of millet subfamily plants may be used in combination. Further, in the present invention, the whole fruit of the millet family may be used, or a part of the fruit separated by processing may be used. Further, the separated parts may be mixed with each other, or the part separated from the actual whole may be mixed and used.

  Processed fruits of millet subfamily plants include those obtained by pulverizing millet subfamily plants, those obtained by pulverizing and adjusting the particle size by sieving, or those formed into granules or pellets. However, in the present invention, it can be used regardless of the processing method of millet subfamily plants, the shape, particle size, etc. of the processed product, and two or more types of processed products may be used in combination. May be used in combination.

  The specific example of the fruit of the millet family in this invention is demonstrated in the case of corn. Corn seeds are composed of scapula, epidermis, endosperm and germ, and endosperm further comprises parts called keratin gluten, keratin starch, and powder. In the present invention, the whole corn (whole grain) or only a part thereof can be used. Moreover, you may mix and use what was divided into parts. Although processing by dry milling will be described as an example of processing of corn seed, the processing method applied in the present invention is not limited to this. In the processing by dry milling of corn, first, impurities are removed in the selection process, and then the water is tempered for the purpose of facilitating separation of the germ. Next, the embryo is removed by using a de-embryo machine, and the pulverized product by a roll mill is sieved according to particle size and then dried to obtain corn grits mainly composed of horny portions and corn flour mainly composed of powdery portions. These are classified into several stages of particle sizes at the screening stage. In addition, by-products in dry milling include germs removed by de-embryo, dry corn germs and dried corn germs and corn fibers consisting of the epidermis, an aleurone layer (glue layer) between the epidermis and endosperm, and epidermis. There is a corn bran made of corn, and a hominy feed that combines all these by-products. In the present invention, any of the products and by-products obtained by the above processing can be used, and these may be mixed and used.

  As mentioned above, although the corn was demonstrated as an example about the fruit of the millet family of the present invention, the present invention is not limited to this example.

  Next, a method for selecting strains will be described.

1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The strain is inoculated and cultured at 25 ° C. for 7 to 14 days, preferably 10 days, to obtain a liquid inoculum.

  On the other hand, millet subfamily seeds, preferably corn nuts and hardwood sawdust, are mixed as a solid culture medium in a dry matter weight ratio of 1: 2 to 3: 1, preferably 2: 1. Thoroughly stirred and mixed with water to 55-65%, preferably 60%, and barley and hardwood sawdust in a dry matter weight ratio of 1: 2-3: 1, preferably 2: 1 (however, , Mix the ratio with the corn medium) and add water so that the water content of the culture medium is finally 55 to 65%, preferably 60% (but match the ratio with the corn medium). Two kinds of well-stirred and mixed ones are prepared and packed into 400 to 2300 ml, preferably 850 or 2300 ml polypropylene wide-mouth culture bottles. In the case of an 850 ml bottle, after filling the whole bottle, a hole with a diameter of about 3 cm is made in the center. In the case of a 2300 ml bottle, the culture medium of the same weight as that filled in the 850 ml bottle is filled to about half of the container, and then about 1 cm in diameter. Drill three holes. After the container is plugged, it is subjected to high-pressure steam sterilization at 101 to 125 ° C. for 30 to 90 minutes, preferably 118 ° C. for 60 minutes, and left to cool.

  The solid inoculum is inoculated with 5 to 30 ml, preferably about 15 ml, preferably about 100 ml, preferably about 50 ml in the case of 850 ml bottle, and in the dark at a temperature of 20 to 25 ° C., preferably in the dark. The mycelium is cultured for about 60 to 150 days, preferably about 100 days under conditions of 23 ° C. and humidity of 40 to 70%, preferably 60 to 70%, and the mycelium is spread throughout the medium. Next, the stopper may be removed and left as it is, but preferably the fungus floor is covered with an appropriate soil covering material, more preferably about 2 cm with red crust sterilized by autoclaving, and then the temperature is 10 to 20 ° C., preferably 15 ° C. It moves to the generation room controlled so that it may become 90-120%, preferably 115-120% by the display value of 100 [made by Kakinomiya Seisakusho Co., Ltd.] Under illumination of 10-1000 lux, preferably 50-500 lux Cover the bottle mouth with a corrugated plate to prevent wetting until the fruiting body primordium is generated, remove the corrugated sheet after the fruiting body primordium formation, continue the culture until the mature fruiting body is obtained, By investigating the fruiting body yield in the strain and the number of days required to move to the germination room, a hon-shimeji strain having good fruiting body formation ability is obtained in the culture medium containing the fruits of the millet family. Door can be. The results thus obtained are shown in Table 1.

  As is clear from Table 1, most of the tested fungi do not occur in the oat culture medium or the fruiting body yield is insufficient, but La 01-20, La 01-27, La 01-37, La 01 As for -45 and La 01-46, high yields are obtained with an inexpensive corn medium, and it can be said that these are excellent varieties suitable for a medium containing the fruits of the millet family. YG6L, which is a known strain described in the Journal of the Mycological Society of Japan, Vol. 39, pp. 13-20 (1998), has a high yield in the oat culture medium, but the corn culture medium has a reduced yield. Since the 01-26 and La 01-38 strains are generated only in the oat medium, the strain compatibility with the corn medium cannot be inferred from the results of the cultivation test on the oat medium alone.

Among the hon-shimeji strains shown in Table 1, the fruiting bodies and spores of those collected from the wild at the time of collection, and the morphological characteristics of the fruiting bodies and spores of each of the generated strains are as follows.
The umbrella has a diameter of 2 to 7 cm and starts from a hemispherical shape to a bun type. The surface of the umbrella is dark at first, and gradually becomes gray to light grayish brown, and the edge is initially strongly wound inside. The meat is white and dense, and the folds are white to light cream and slightly hang. The stem is white and the bottom is in the shape of a bottle of liquor, but some fruit bodies that have grown sufficiently are nearly the same size. The spores are spherical and have a diameter of 4 to 6 μm.
Comparing the above characteristics with that described by Rokuya Imeki and Tsujio Hongo, “Primary-color Japanese New Fungi Encyclopedia (I)”, Koikusha (published first edition on June 10, 1987), these strains are found to be hon-shimeji. It is clear.

  Among these test strains, five strains of La 01-20, La 01-27, La 01-37, La 01-45, and La 01-46 are Lyophyllum shimeji La 01-20, Lyophyllum shimeji La 01-27, Lyophyllum shimeji La 01-37, Lyophyllum shimeji La 01-45, Lyophyllum shimeji La 01-46 are displayed at the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology, FERM P-16541, FERM P-17455, FERM P-17456, It has been deposited as FERM P-17457 and FERM P-17458.

Next, the mycological properties of 5 strains of Honshimeji La 01-20, La 01-27, La 01-37, La 01-45, and La 01-46 are described below.
(1) Honshimeji La 01-20 strain A. Growth state on malt extract agar medium (25 ° C.) On the 7th day, the colony diameter is 30 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 46 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 69 mm, and white and dense hyphae and aerial hyphae are few. Mycelium extends radially.
B. Growth state in potato / glucose agar medium (25 ° C.) On day 7, colony diameter is 29 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 44 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 70 mm, and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially. The center of the back is slightly colored.
C. Growth state on Czapek Docs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, white and dilute mycelia and aerial hyphae are very few.
On the 10th day, the colony diameter was 10 mm.
On the 17th day, the colony diameter is 13 mm, the white and thin mycelia and dendrites grow, and the aerial hyphae are very few.
D. Growth condition on Sabouraud agar medium (25 ° C.) On the 7th day, the colony diameter is 11 mm, and white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 14 mm, and white and thin mycelia and aerial mycelia are few.
On the 17th day, the colony diameter is 23 mm, the peripheral part is white and dilute mycelia, the center part is slightly dense and slightly colored, and there are few aerial hyphae, and the colony shape is irregular.
E. Growth state on oatmeal agar medium (25 ° C.) On the 7th day, colony diameter is 19 mm, white and dense hyphae, and aerial hyphae are few.
On the 10th day, the colony diameter is 32 mm, white and dense hyphae and aerial hyphae are few.
On the 17th day, the colony diameter is 59 mm, and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially.
F. Growth state on synthetic mucor agar medium (25 ° C.) On the 7th day, colony diameter is 17 mm, white and thin hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 25 mm, and there are few white and thin mycelia and aerial hyphae.
On the 17th day, the colony diameter is 42 mm, and white and thin mycelia and aerial hyphae are few. The back side is slightly colored.
G. Growth state on YpSs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 10 mm, and there are few white, mat-like dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 17 mm, and there are few white, mat-like dense hyphae and aerial hyphae.
H. Growth state on sugar yeast agar medium (25 ° C.) On day 7, the colony diameter is 23 mm, white and dense mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 35 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 58 mm, white, the center is dense, and the peripheral edge is thin. Wrinkles occur in the center of the colony.
I. Growth state in phenol oxidase assay medium (25 ° C.) Use potato / glucose agar medium supplemented with 0.5% gallic acid. On the 7th day, there was no growth at all, and the diameter of the browning area was 18 mm.
J. et al. Optimal growth temperature PGY agar medium was inoculated with 6 mm diameter inoculum, cultured at 5, 10, 15, 20, 25, 30 and 35 ° C., and each colony diameter was measured after 12 days. It was around 25 ° C. Moreover, it did not grow at 35 degreeC.
K. Optimum growth pH
Prepare 40 ml of PGY liquid medium in a 100 ml Erlenmeyer flask, and after sterilization, aseptically with 1 N hydrochloric acid or 1 N sodium hydroxide solution, pH 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, inoculated with 6 mm diameter inoculum, When the dry weight was measured after standing for 15 days, the optimum growth pH was around 5.5. The growth range of this strain was in the range of pH 3.0 to 7.5.

(2) Honshimeji La 01-27 strain A. Growth state on malt extract agar medium (25 ° C.) On the 7th day, the colony diameter is 32 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 46 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 69 mm, and white and dense hyphae and aerial hyphae are few. Mycelium extends radially.
B. Growth condition on potato / glucose agar medium (25 ° C.) On day 7, colony diameter is 27 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 40 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 70 mm, and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially. Slight wrinkles in the center of the back side.
C. Growth condition on Czapek Docs agar medium (25 ° C.) On the 7th day, the colony diameter is 7 mm, white and dilute mycelia and aerial hyphae are very few.
On the 10th day, the colony diameter was 9 mm.
On the 17th day, the colony diameter is 10 mm, white and dilute mycelia and dendrites, and there are very few aerial mycelia.
D. Growth condition on Sabouraud agar medium (25 ° C.) On the 7th day, the colony diameter is 12 mm, and white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 16 mm, and there are few white and thin mycelia and aerial hyphae.
On day 17, the colony diameter is 32 mm, the peripheral edge is white and thin mycelia, the center is slightly dense and slightly colored, and there are few aerial mycelia.
E. Growth state on oatmeal agar medium (25 ° C.) On the 7th day, the colony diameter is 21 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 33 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 55 mm, white and dense hyphae and aerial hyphae are few.
F. Growth condition on synthetic mucor agar medium (25 ° C.) On the 7th day, colony diameter is 22 mm, white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 32 mm, and white and thin mycelium and aerial mycelia are few.
On the 17th day, the colony diameter is 50 mm, and white and thin mycelia and aerial mycelia are few.
G. Growth state on YpSs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 10 mm, and there are few white, mat-like dense hyphae and aerial hyphae.
On the 17th day, the colony diameter was 17 mm, white, mat-like dense hyphae, and the central part was slightly colored. There are few aerial hyphae.
H. Growth condition on sugar yeast agar medium (25 ° C.) On the 7th day, colony diameter is 24 mm, white and dense hyphae, aerial hyphae are few.
On the 10th day, the colony diameter is 36 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 62 mm, white, the center is dense, and the peripheral edge is thin. Wrinkles occur in the center of the colony.
I. Growth state in phenol oxidase assay medium (25 ° C.) Use potato / glucose agar medium supplemented with 0.5% gallic acid. On the 7th day, there was no growth at all, and the diameter of the browning area was 12 mm.
J. et al. Optimal growth temperature PGY agar medium was inoculated with an inoculum of 6 mm in diameter, cultured at each temperature described in (1) -J, and measured for each colony diameter after 12 days. The optimal growth temperature was around 25 ° C. there were. Moreover, it did not grow at 35 degreeC.
K. Optimum growth pH
40 ml of PGY liquid medium was prepared in a 100 ml Erlenmeyer flask, and after sterilization, the pH was adjusted in the same manner as in section (1) -K, inoculated with an inoculum of 6 mm in diameter, allowed to stand for 15 days, and each dry weight was measured. The optimum growth pH was around 5.0. The growth range of this strain was in the range of pH 3.0 to 8.0.

(3) Honshimeji La 01-37 strain A. Growth state on malt extract agar medium (25 ° C.) On day 7, the colony diameter is 28 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 40 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 67 mm, white and dense mycelia and aerial hyphae are few. Mycelium extends radially.
B. Growth state on potato / glucose agar medium (25 ° C.) On day 7, colony diameter is 28 mm, white and dense mycelia, and aerial hyphae are few.
On the 10th day, the colony diameter is 40 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 66 mm and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially.
C. Growth state on Czapek Docs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, white and dilute mycelia and aerial hyphae are very few.
On the 10th day, the colony diameter was 10 mm.
On the 17th day, the colony diameter is 13 mm, the white and thin mycelia and dendrites grow, and the aerial hyphae are very few.
D. Growth state on Sabouraud agar medium (25 ° C.) On the 7th day, the colony diameter is 19 mm, and white and thin mycelia and aerial mycelia are few.
On the 10th day, the colony diameter is 27 mm, and white and thin mycelia and aerial hyphae are few.
On the 17th day, the colony diameter is 40 mm, the peripheral edge is white and thin mycelia, the center is slightly dense and colored, and there are few aerial hyphae.
E. Growth state on oatmeal agar medium (25 ° C.) On the 7th day, the colony diameter is 20 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 29 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 54 mm, white and dense mycelia and aerial hyphae are few.
F. Growth state on synthetic mucor agar medium (25 ° C.) On the 7th day, colony diameter is 20 mm, white and thin hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 25 mm, and there are few white and thin mycelia and aerial hyphae.
On the 17th day, the colony diameter is 42 mm, and white and thin mycelia and aerial hyphae are few.
G. Growth state on YpSs agar medium (25 ° C.) On the 7th day, the colony diameter is 10 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 12 mm, and there are few white, mat-like dense hyphae and aerial hyphae.
On the 17th day, the colony diameter was 21 mm, white, mat-like dense hyphae, and the central part was slightly colored. There are few aerial hyphae.
H. Growth condition on sugar yeast agar medium (25 ° C.) On the 7th day, the colony diameter is 22 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 31 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 56 mm, white, the central part is dense, and the peripheral part is thin. Wrinkles occur in the center of the colony.
I. Growth state in phenol oxidase assay medium (25 ° C.) Use potato / glucose agar medium supplemented with 0.5% gallic acid. On the 7th day, there was no growth at all, and the diameter of the browning area was 12 mm.
J. et al. Optimal growth temperature PGY agar medium was inoculated with an inoculum of 6 mm in diameter, cultured at each temperature described in (1) -J, and measured for each colony diameter after 12 days. The optimal growth temperature was around 25 ° C. there were. Moreover, it did not grow at 35 degreeC.
K. Optimum growth pH
40 ml of PGY liquid medium was prepared in a 100 ml Erlenmeyer flask, and after sterilization, the pH was adjusted in the same manner as in section (1) -K, inoculated with an inoculum of 6 mm in diameter, allowed to stand for 15 days, and each dry weight was measured. The optimum growth pH was around 5.5. The growth range of this strain was in the range of pH 3.0 to 8.0.

(4) Honshimeji La 01-45 strain A. Growth condition on malt extract agar medium (25 ° C.) On the 7th day, the colony diameter is 27 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 40 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 69 mm, and white and dense hyphae and aerial hyphae are few. Mycelium extends radially.
B. Growth state in potato / glucose agar medium (25 ° C.) On day 7, colony diameter is 24 mm, white and dense hyphae, few aerial hyphae.
On the 10th day, the colony diameter is 36 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 66 mm and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially. The center of the back is slightly colored.
C. Growth state on Czapek Docs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, white and dilute mycelia and aerial hyphae are very few.
On the 10th day, the colony diameter was 10 mm.
On the 17th day, the colony diameter is 12 mm, white and dilute mycelia and dendrites, and there are very few aerial mycelia.
D. Growth state on Sabouraud agar medium (25 ° C.) On the 7th day, the colony diameter is 18 mm, and white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 24 mm, and white and thin mycelia and aerial mycelia are few.
On the 17th day, the colony diameter is 38 mm, the peripheral part is white and thin mycelia, the central part is dense, slightly colored, distorted colonies, and there are few aerial mycelia.
E. Growth state on oatmeal agar medium (25 ° C.) On the 7th day, the colony diameter is 14 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 30 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 57 mm, white and dense mycelia and aerial hyphae are few.
F. Growth state on synthetic mucor agar medium (25 ° C.) On day 7, colony diameter is 14 mm, white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 20 mm, and there are few white and thin mycelia and aerial mycelia.
On the 17th day, the colony diameter is 33 mm, the white and thin mycelium, the central mycelium is dense, and there are few aerial mycelia. The back center is slightly colored.
G. Growth state on YpSs agar medium (25 ° C.) On the 7th day, the colony diameter is 11 mm, and white and mat-like dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 16 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 17th day, the colony diameter is 36 mm, and white, mat-like dense hyphae and aerial hyphae are few.
H. Growth state on sugar yeast agar medium (25 ° C.) On the 7th day, colony diameter is 25 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 35 mm and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 63 mm, white, dense in the center and thin in the periphery, and there are few hyphae and aerial hyphae. Wrinkles occur in the center of the colony.
I. Growth state in phenol oxidase assay medium (25 ° C.) Use potato / glucose agar medium supplemented with 0.5% gallic acid. On the 7th day, there was no growth and the browning area was 22 mm in diameter.
J. et al. Optimal growth temperature PGY agar medium was inoculated with an inoculum of 6 mm in diameter, cultured at each temperature described in (1) -J, and measured for each colony diameter after 12 days. The optimal growth temperature was around 25 ° C. there were. Moreover, it did not grow at 35 degreeC.
K. Optimum growth pH
40 ml of PGY liquid medium was prepared in a 100 ml Erlenmeyer flask, and after sterilization, the pH was adjusted in the same manner as in section (1) -K, inoculated with an inoculum of 6 mm in diameter, allowed to stand for 15 days, and each dry weight was measured. The optimum growth pH was around 5.5. The growth range of this strain was in the range of pH 3.5 to 7.0.

(5) Honshimeji La 01-46 strain A. Growth state on malt extract agar medium (25 ° C.) On day 7, colony diameter is 29 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 41 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 56 mm and there are few white and dense hyphae and aerial hyphae. Mycelium extends radially.
B. Growth condition on potato / glucose agar medium (25 ° C.) On day 7, colony diameter is 27 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 40 mm, and there are few white and dense hyphae and aerial hyphae.
On day 17, the colony diameter is 54 mm, white and dense mycelia, the front and back surfaces are slightly colored, and there are few aerial mycelia. Mycelium extends radially.
C. Growth state on Czapek Docs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, white and dilute mycelia and aerial hyphae are very few.
On the 10th day, the colony diameter was 11 mm.
On the 17th day, the colony diameter is 12 mm, white and dilute mycelia and dendrites, and there are very few aerial mycelia.
D. Growth condition on Sabouraud agar medium (25 ° C.) On the 7th day, the colony diameter is 17 mm, white and dilute mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 25 mm, and there are few white and thin mycelia and aerial hyphae.
On the 17th day, the colony diameter is 36 mm, the peripheral part is white and thin mycelia, the central part is slightly dense and slightly colored, and there are few aerial mycelia.
E. Growth state on oatmeal agar medium (25 ° C.) On the 7th day, the colony diameter is 17 mm, white and dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 31 mm, and there are few white and dense hyphae and aerial hyphae.
On the 17th day, the colony diameter is 47 mm and there are few white and dense mycelia and aerial hyphae. Mycelium extends radially.
F. Growth state on synthetic mucor agar medium (25 ° C.) On the 7th day, colony diameter is 21 mm, white and thin hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 28 mm, and white and thin mycelia and aerial hyphae are few.
On the 17th day, the colony diameter is 36 mm, and white and thin mycelium and aerial mycelia are few.
G. Growth state on YpSs agar medium (25 ° C.) On the 7th day, the colony diameter is 8 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 10th day, the colony diameter is 11 mm, and white, mat-like dense hyphae and aerial hyphae are few.
On the 17th day, the colony diameter is 16 mm, and there are few white, mat-like dense hyphae and aerial hyphae.
H. Growth state on sugar yeast agar medium (25 ° C.) On day 7, the colony diameter is 23 mm, white and dense mycelia and aerial hyphae are few.
On the 10th day, the colony diameter is 32 mm, white and dense hyphae and aerial hyphae are few.
On the 17th day, the colony diameter is 44 mm, white, dense in the center and thin in the periphery, and there are few hyphae and aerial hyphae. Wrinkles occur in the center of the colony.
I. Growth state in phenol oxidase assay medium (25 ° C.) Use potato / glucose agar medium supplemented with 0.5% gallic acid. On the 7th day, there was no growth, and the browning area was 18 mm in diameter.
J. et al. Optimal growth temperature PGY agar medium was inoculated with 6 mm diameter inoculum, cultured at each temperature described in (1) -J, and after 12 days, each colony diameter was measured. The optimal growth temperature was around 25 ° C. Met. Moreover, it did not grow at 35 degreeC.
K. Optimum growth pH
40 ml of PGY liquid medium was prepared in a 100 ml Erlenmeyer flask, and after sterilization, the pH was adjusted in the same manner as in section (1) -K, inoculated with an inoculum of 6 mm in diameter, allowed to stand for 15 days, and each dry weight was measured. The optimum growth pH was around 5.0. The growth range of this strain was in the range of pH 3.0 to 7.5.

  Further, the difference between Honshimeji La 01-20, La 01-27, La 01-37, La 01-45, La 01-46 and other Honshimeji strains can be examined by conducting counterculture on an agar medium. it can. The mycelium of the test strain was cut out as a 3 mm x 3 mm x 3 mm block from the PGY agar medium, inoculated at 2 cm intervals in the center of the PGY agar plate medium, cultured at 25 ° C for 15 days, and the boundary between both colonies It is determined whether or not a band is generated. The results obtained by testing 20 Honshimeji strains in this manner are shown in Table 2 as + when the band is generated and-when the band is not generated. In addition, the thing of the antagonistic state which is not colored here is also included here.

  As shown in Table 2, Honshimeji La 01-20, La 01-27, La 01-37, La 01-45, and La 01-46 formed a band with all the strains tested. It is clear that it is a new strain.

  As cultivation methods of the Honshimeji strain of the present invention, bottle cultivation, bag cultivation, box cultivation and the like can be applied. When bottle cultivation is described as an example, the method includes media preparation, bottle filling, sterilization, inoculation, culture, sprouting, growth, and harvesting.

  Next, although these are demonstrated concretely, this invention is not limited to these.

  Medium preparation refers to a process in which various substrates used for artificial cultivation are weighed, stirred, hydrated to adjust water content. The culture medium for artificial cultivation of hon-shimeji mushroom used in the present invention comprises a combination of millet subfamily seeds, sawdust, and other nutrients. As an example, the mixing ratio of millet subfamily seeds and other medium base materials is preferably 1 part by weight or more by dry weight ratio of corn seeds to other base materials. However, the present invention is not limited to this ratio. Although the preparation of the medium in the present invention has been described with corn seeds, the present invention is not limited to this, and any millet subfamily can be used.

  The bottling is a process of filling the culture medium in a bottle, and usually the culture medium prepared in a 400 to 2300 ml heat-resistant wide-mouth culture bottle is filled with 500 to 800 g, preferably 600 to 750 g in the case of an 850 ml bottle, It refers to the process of punching one or more holes of about 1 to 3 cm in the center.

  The sterilization may be a step of killing all microorganisms in the culture medium by steam, and is usually 98-100 ° C. for 4-12 hours for normal pressure sterilization, 101-125 ° C., preferably 118 ° C., 30 for high pressure sterilization. Performed for ~ 90 minutes.

  Inoculation is a process of inoculating inoculum on a medium that has been allowed to cool after sterilization. As normal inoculum, hon-shimeji mycelium is cultured in PGY liquid medium, 1/2 PGY liquid medium, etc. at 25 ° C. for 10 to 15 days, Aseptically plant about 10-50 ml per bottle. Moreover, the culture medium inoculated with the liquid inoculum obtained in the steps described so far is cultured at 25 ° C. for 60 to 150 days, and the bacteria are used as a solid inoculum, and aseptically planted by about 15 g per bottle.

  Culture is a process of growing and aging mycelia, in which the inoculated culture medium is spread at a temperature of 20 to 25 ° C. and a humidity of 40 to 70%, and further aged. Aging can be omitted. In the case of an 850 ml bottle, the culture step is usually performed for 60 to 150 days, preferably about 100 days.

  Sprouting is a step of removing the stopper after the culturing process to form a fruiting body primordium, usually 10 to 20 ° C., preferably around 15 ° C., humidity 80% or more, and illuminance 1000 lux or less for 10 to 20 days. Do. At this time, the fungus floor may be covered with an appropriate material. Examples of soil covering materials include red ores, Kanuma soil, vermiculite, quartz, perlite, glass beads and other inorganic minerals, and soils such as field soil, forest soil, and mountain soil, which are fine particles. However, the present invention is not limited to the particle size of the covering material. Or humic materials such as Hyuga soil, heat-hardened red jade soil and other humic materials such as humus, bark compost, peat moss, or coniferous sawdust, hardwood sawdust, corn cob, cellulose pulp, cellulose sponge, rice husk, rice Plant materials such as straw, sphagnum, and agar residue produced as a by-product during agar production are also examples of the soil covering material. These covering materials may be used alone or in combination of two or more. Further, the soil covering material may be used after absorbing water in advance so as to have an appropriate moisture content.

  In addition, after removing the stopper, a fungus scraping operation may be performed to scrape off the inoculum portion and the culture medium surface. Further, after removing the stopper or scraping the fungus, water may be immediately added to the bottle mouth to feed the culture medium, and after 3 to 5 hours, a hydration operation may be performed to drain the excess water. In addition, during the sprouting process, dew condensation is likely to occur due to humidification, and therefore the fungus floor may be covered with a perforated polysheet or corrugated sheet for the purpose of preventing wetting.

  Growth is a process of forming mature fruit bodies from the fruit body primordium, and is performed for 5 to 15 days under substantially the same conditions as the normal sprouting process. In the growth process, it is difficult to be affected by wetting with dew condensation water, and therefore it is preferable not to coat with a porous polysheet or corrugated sheet.

  A mature fruit body can be obtained by the above process, harvesting is performed, and all the processes of cultivation are complete | finished.

  As mentioned above, although this invention was demonstrated by the bottle cultivation method, this invention is not limited to the said bottle cultivation.

  According to the present invention, it is possible to commercially artificially cultivate hon-shimeji mushrooms with good yield and shape using a culture medium using an inexpensive medium raw material. Examples of suitable Honshimeji strains used in the present invention include Honshimeji La 01-20, Honshimeji La 01-27, Honshimeji La 01-37, Honshimeji La 01-45, Honshimeji La 01-46, And strains that do not form a band by counter-culture with these strains as parent strains, but the present invention is not limited to these strains. Even if it is a strain that forms a line, it is a hon-shimeji strain that has a good fruiting ability in a culture medium containing seeds of millet subfamily plants, a strain that has been selected and isolated from nature, mating, mutation induction, protoplast Any Honshimeji strain that is bred by treatment, cell fusion, genetic manipulation, etc. and can be used for artificial cultivation is included in the present invention. It is.

  In addition, by cultivating these strains in a culture medium containing the seeds of millet subfamily plants according to the present invention, the number of days from moving to the harvesting room to harvesting can be shortened compared to when cultivating in a culture medium using wheat. It becomes possible to do.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited only to the scope of the following examples.

Example 1
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The mycelium of 01-20 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured for 108 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The corn medium was cultured for 26 days and the oat medium was cultured for 27 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the corn medium is 123.0 g, and the average yield per bottle of the mature fruit body obtained from the oat medium is 83.8 g. Corn media was superior in both days.

Example 2
In a wide-mouth culture bottle made of polypropylene (2300 ml), maize seeds (excluded from the pulverized feed for Ito Seiwa feed), or oats (Toyohashi Food Industry Co., Ltd.) and hardwood 850 ml of Example 1 was prepared by mixing the sawdust (Tomoe product) with a dry matter weight ratio of 2: 1, adding water so that the water content of the culture medium was finally 60%, and thoroughly stirring and mixing. After filling the bottle with the same weight as about half of the container, open three holes with a diameter of about 1cm, plug and sterilize by autoclaving at 118 ° C for 60 minutes. Prepared as culture medium.

  About 50 ml of the liquid inoculum described in Example 1 was inoculated into this solid culture medium, and the mycelium was cultured in a dark place at a temperature of 23 ° C. and a humidity of 60 to 70% for 81 days to spread the mycelium throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The corn medium was cultured for 26 days and the oat medium was continued for 29 days to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained from corn medium is 153.0 g, and the average yield per bottle of mature fruit bodies obtained from oat medium is 98.5 g. Corn media was superior in both days.

Example 3
In a wide-mouth culture bottle made of polypropylene (for 800 ml nameko), corn nuts (excluded from the pulverized feed for Ito barley), or oats (Toyohashi Food Industry Co., Ltd.) Hardwood sawdust [(Tomoe product)] is mixed at a dry matter weight ratio of 2: 1, and water is added so that the final moisture content of the culture medium is 60%. After filling about half of the volume, a hole having a diameter of about 1.5 cm was made in the center, and then plugged, and pasteurized at 118 ° C. for 60 minutes under high pressure steam and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 1 was inoculated into this solid culture medium, and mycelia were cultured for 63 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelia were spread throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Both corn medium and oat medium were cultured for 29 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained in the corn medium is 105.3 g, and the average yield per bottle of the mature fruit body obtained in the oat medium is 82.5 g. The corn medium is superior in yield. It was.

Example 4
In a wide-mouth culture bottle made of polypropylene (for 800ml nameko), heat-pressed corn and corn (Ito Seiya) or Oshigi (Toyohashi Shokuhin Kogyo Co., Ltd.) and hardwood sawdust (Tomoe Bussan Co., Ltd.) in a dry matter weight ratio of 2 1: Mix and mix well so that the water content of the culture medium finally becomes 60%. Stir and mix well, and fill about half of the bottle volume. After being opened, it was stoppered, sterilized by high-pressure steam at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 1 was inoculated into this solid culture medium, and mycelia were cultured for 63 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelia were spread throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The culture was continued for 29 days for both the hot-pressed corn medium and the oat medium to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained with the heated pressed corn medium was 87.5 g, and the average yield per bottle of the mature fruit body obtained with the oat medium was 82.5 g. The corn medium was superior.

Example 5
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The mycelium of 01-27 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 109 days, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 32 days to obtain mature fruiting bodies. The average yield per bottle of the mature fruit body obtained in the corn medium was 85.0 g. The oat culture medium showed no fruiting, and the corn culture medium was superior.

Example 6
In a wide-mouth culture bottle made of polypropylene (2300 ml), maize seeds (excluded from the pulverized feed for Ito Seiwa feed), or oats (Toyohashi Food Industry Co., Ltd.) and hardwood In Example 5, 850 ml of the sawdust (Tomoe product) was mixed at a dry matter weight ratio of 2: 1 and water was added so that the water content of the culture medium was finally 60%. After filling the bottle with the same weight as about half of the container, open three holes with a diameter of about 1cm, plug and sterilize by autoclaving at 118 ° C for 60 minutes. Prepared as culture medium.

  About 50 ml of the liquid inoculum described in Example 5 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 109 days, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culture was continued for 25 days to obtain mature fruiting bodies. The average yield per bottle of mature fruit bodies obtained on corn medium was 145.5 g. The oat culture medium showed no fruiting, and the corn culture medium was superior.

Example 7
In a wide-mouth culture bottle made of polypropylene (for 800 ml nameco), corn nuts (Ito barley) are mixed with feed pulverized food and usually mixed with fish meal, or oats (Toyohashi Food Industry Co., Ltd.) And hardwood sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. About half amount was packed, a hole having a diameter of about 1.5 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 5 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 112 days, and the mycelium was spread throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The corn medium was cultured for 20 days and the oat medium was cultured for 22 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the corn medium is 94.4 g, and the average yield per bottle of the mature fruit body obtained from the oat medium is 69.2 g. Corn media was superior in both days.

Example 8
In a wide-mouth culture bottle made of polypropylene (for 800ml nameko), heat-pressed corn and corn (Ito Seiya) or Oshigi (Toyohashi Shokuhin Kogyo Co., Ltd.) and hardwood sawdust (Tomoe Bussan Co., Ltd.) in a dry matter weight ratio of 2 1: Mix, mix well and mix well so that the water content of the culture medium finally becomes 60%. Pack about half of the bottle volume, and open a hole with a diameter of about 1.5 cm in the center. After that, it was stoppered, sterilized by high-pressure steam at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 5 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 112 days, and the mycelium was spread throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 21 days on a heated corn and corn medium and 22 days on an oat medium to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained with a heated pressed corn medium was 79.3 g, and the average yield per bottle of mature fruit bodies obtained with an oat medium was 69.2 g. The heated pressed pieces and the corn medium were superior in the later required days.

Example 9
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The mycelium of 01-37 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  In a wide-mouth culture bottle made of polypropylene (2300 ml), corn nuts (excluding ground fish meal mixed with Ito Seiyaku Co., Ltd.) or oats (Toyohashi Food Industry Co., Ltd.) and hardwood 850 ml of Example 1 was prepared by mixing the sawdust (Tomoe product) with a dry matter weight ratio of 2: 1, adding water so that the water content of the culture medium was finally 60%, and thoroughly stirring and mixing. After filling the bottle with the same weight as about half of the container, open three holes with a diameter of about 1cm, plug and sterilize by autoclaving at 118 ° C for 60 minutes. Prepared as culture medium.

  About 50 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured for 81 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 27 days to obtain mature fruiting bodies. The average yield per bottle of mature fruit bodies obtained in corn medium was 90.0 g. The oat culture medium showed no fruiting, and the corn culture medium was superior.

Example 10
In a wide-mouth culture bottle made of polypropylene (for 800 ml nameko), corn nuts (excluded from the pulverized feed for Ito barley), or oats (Toyohashi Food Industry Co., Ltd.) Hardwood sawdust [(Tomoe product)] is mixed at a dry matter weight ratio of 2: 1, and water is added so that the final moisture content of the culture medium is 60%. After filling about half of the volume, a hole having a diameter of about 1.5 cm was made in the center, and then plugged, and pasteurized at 118 ° C. for 60 minutes under high pressure steam and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 9 was inoculated into this solid culture medium, and the mycelium was cultured for 112 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclaving, the generation chamber was controlled so that the temperature was 15 ° C. and the humidity was 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). Cover the bottle mouth with corrugated plate to prevent wetting of the fruit body under the illumination of 50 to 500 lux until the fruit body primordium is generated. The corn medium was cultured for 25 days and the oat medium was cultured for 26 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the corn medium is 65.3 g, and the average yield per bottle of the mature fruit body obtained from the oat medium is 56.0 g. Corn media was superior in both days.

Example 11
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The mycelium of 01-45 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured for 112 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The culture was continued for 30 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained in corn medium was 79.0 g. In the oat culture medium, no fruiting body was observed, and the corn culture medium was superior in yield and the number of days required after moving to the generation room.

Example 12
In a wide-mouth culture bottle made of polypropylene (2300 ml), maize seeds (excluded from the pulverized feed for Ito Seiwa feed), or oats (Toyohashi Food Industry Co., Ltd.) and hardwood 850 ml of Example 1 was prepared by mixing the sawdust (Tomoe product) with a dry matter weight ratio of 2: 1, adding water so that the water content of the culture medium was finally 60%, and thoroughly stirring and mixing. After filling the bottle with the same weight as about half of the container, open three holes with a diameter of about 1cm, plug and sterilize by autoclaving at 118 ° C for 60 minutes. Prepared as culture medium.

  About 50 ml of the liquid inoculum described in Example 11 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 84 days to spread the mycelium throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 33 days on corn medium and 39 days on pressed oat medium to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the corn medium is 112.0 g, and the average yield per bottle of the mature fruit body obtained from the oat medium is 44.0 g. Corn media was superior in both days.

Example 13
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) A mycelium of 01-46 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured for 112 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 29 days to obtain mature fruiting bodies. The average yield per bottle of the mature fruit body obtained in the corn medium was 78.9 g. The oat culture medium showed no fruiting, and the corn culture medium was superior.

Example 14
In a wide-mouth culture bottle made of polypropylene (for 800 ml nameko), corn nuts (excluded from the pulverized feed for Ito barley), or oats (Toyohashi Food Industry Co., Ltd.) Hardwood sawdust [(Tomoe product)] is mixed at a dry matter weight ratio of 2: 1, and water is added so that the final moisture content of the culture medium is 60%. After filling about half of the volume, a hole having a diameter of about 1.5 cm was made in the center, and then plugged, and pasteurized at 118 ° C. for 60 minutes under high pressure steam and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 13 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 105 days to spread the mycelium throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclaving, the generation chamber was controlled so that the temperature was 15 ° C. and the humidity was 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). Cover the bottle mouth with corrugated plate to prevent wetting of the fruit body under the illumination of 50 to 500 lux until the fruit body primordium is generated. The corn medium was cultured for 26 days and the oat medium was continued for 29 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the corn medium is 62.5 g, and the average yield per bottle of the mature fruit body obtained from the oat medium is 27.8 g. Corn media was superior in both days.

Example 15
In a wide-mouth culture bottle made of polypropylene (for 800ml nameko), heat-pressed corn and corn (Ito Seiya) or Oshigi (Toyohashi Shokuhin Kogyo Co., Ltd.) and hardwood sawdust (Tomoe Bussan Co., Ltd.) in a dry matter weight ratio of 2 1: Mix, mix well and mix well so that the water content of the culture medium finally becomes 60%. Pack about half of the bottle volume, and open a hole with a diameter of about 1.5 cm in the center. After that, it was stoppered, sterilized by high-pressure steam at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 13 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 105 days to spread the mycelium throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The hot pressed corn medium and the oat medium were cultured for 34 days and 29 days, respectively, to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained with a heated pressed corn medium was 35.3 g, and the average yield per bottle of mature fruit bodies obtained with an oat medium was 27.8 g. The corn medium was superior.

Example 16
In a wide-mouth culture bottle made of polypropylene (for 800ml nameko), whole grain milo [Osaka Emerging Feed] or Oshigi [Toyohashi Foods Industry Co., Ltd.] and hardwood sawdust ((Tomoe) Co., Ltd.) in a dry weight ratio of 2: 1 After mixing, add water so that the water content of the culture medium finally becomes 60%, and stir and mix well. Pack about half of the bottle volume and punch a hole about 1.5cm in diameter in the center. The mixture was stoppered, subjected to high-pressure steam sterilization at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 13 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 105 days to spread the mycelium throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The whole grain mylo medium was cultured for 30 days, and the oat medium was continued for 29 days to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained with whole grain mylo medium is 31.3 g, and the average yield per bottle of mature fruit bodies obtained with oat medium is 27.8 g. Was better.

Example 17
In a wide-mouth culture bottle made of polypropylene (for 800ml nameko), millet seeds [Osaka Emerging Feed] or Oshiba [Toyohashi Foods Industry Co., Ltd.] and hardwood sawdust [(Yes) Tomoe Bussan] in a weight ratio of 2: 1 After mixing, add water so that the water content of the culture medium finally becomes 60%, and stir and mix well. Pack about half of the bottle volume and punch a hole about 1.5cm in diameter in the center. The mixture was stoppered, subjected to high-pressure steam sterilization at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 13 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 105 days to spread the mycelium throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The millet medium was cultured for 33 days and the oat medium was continued for 29 days to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained with millet medium is 29.0 g, and the average yield per bottle of mature fruit bodies obtained with oat medium is 27.8 g. It was.

Example 18
In a wide-mouth culture bottle made of polypropylene (for 800 ml nameko), millet (Osaka Emerging Feed) or Oshiba (Toyohashi Foods Industry Co., Ltd.) and hardwood sawdust ((Tomoe) Co., Ltd.) in a dry weight ratio of 2: 1 After mixing, add water so that the water content of the culture medium finally becomes 60%, and stir and mix well. Pack about half of the bottle volume and punch a hole about 1.5cm in diameter in the center. The mixture was stoppered, sterilized by high-pressure steam at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 20 ml of the liquid inoculum described in Example 13 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 105 days to spread the mycelium throughout the medium. . Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. The millet was continued for 27 days and the pressed barley medium for 29 days to obtain mature fruit bodies. The average yield per bottle of mature fruit bodies obtained with millet medium is 49.3 g, and the average yield per bottle of mature fruit bodies obtained with Oshimugi medium is 27.8 g. The millet culture medium was superior in both days.

Comparative Example 1
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) The mycelium of 01-26 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 109 days, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 27 days to obtain mature fruiting bodies. The average yield per bottle of the mature fruit body obtained from the oat medium was 42.0 g. In the corn medium, no fruiting body was observed, and the oat medium was superior.

Comparative Example 2
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) A mycelium of 01-38 strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 109 days, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 35 days to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained from the oat medium was 76.0 g. In the corn medium, no fruiting body was observed, and the oat medium was superior.

Comparative Example 3
1/2 PGY liquid medium (composition: glucose 1.0%, peptone 0.1%, yeast extract 0.1%, KH ₂ PO ₄ 0.025%, MgSO ₄ .7H ₂ O 0.025%) in 200 ml Hongshimeji YG6L The mycelium of the strain was inoculated and cultured at 25 ° C. for 10 days to obtain a liquid inoculum.

  On the other hand, in a wide-mouth culture bottle made of polypropylene (850 ml), corn nuts (excluded from the pulverized feed for Ito Seiyaku), or oats (Toyohashi Food Industry Co., Ltd.) And broad-leaved sawdust (Tomoe product) are mixed at a dry matter weight ratio of 2: 1, and water is added so that the water content of the culture medium is finally 60%. A hole having a diameter of about 3 cm was made in the center, and then plugged, and autoclaved at 118 ° C. for 60 minutes, and allowed to cool to prepare a solid culture medium.

  About 15 ml of the above liquid inoculum was inoculated into this solid culture medium, and the mycelium was cultured for 123 days in the dark at a temperature of 23 ° C. and a humidity of 60 to 70%, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 29 days in the corn medium and 37 days in the oat medium to obtain mature fruit bodies. The average yield per bottle of the mature fruit body obtained in the corn medium is 34.3 g, and the average yield per bottle of the mature fruit body obtained in the corn medium is 78.8 g. It was.

Comparative Example 4
In a wide-mouth culture bottle made of polypropylene (2300 ml), corn nuts (excluding ground fish meal mixed with Ito Seiyaku Co., Ltd.) or oats (Toyohashi Food Industry Co., Ltd.) and hardwood 850 ml of Comparative Example 3 was prepared by mixing sawdust [Tomoe product] (2) in a dry matter weight ratio of 2: 1, adding water so that the water content of the culture medium was finally 60%, and thoroughly stirring and mixing. After filling the bottle with the same weight as about half of the container, open three holes with a diameter of about 1cm, plug and sterilize by autoclaving at 118 ° C for 60 minutes. Prepared as culture medium.

  About 50 ml of the liquid inoculum described in Comparative Example 3 was inoculated into this solid culture medium, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 77 days, and the mycelium was spread throughout the medium. Next, after removing the stopper and covering the bottle mouth with red clay sterilized by autoclave, the temperature is 15 ° C., and the humidification is controlled to be 115 to 120% as indicated by Humiai 100 (manufactured by Kakinomiya Seisakusho). The bottle mouth is covered with a corrugated sheet to prevent water wetting until the fruit body primordium is generated under illumination of 50 to 500 lux. Culturing was continued for 28 days to obtain mature fruiting bodies. The average yield per bottle of the mature fruit body obtained in the corn medium is 74.1 g, and the average yield per bottle of the mature fruit body obtained in the pressed wheat medium is 145.1 g, and the yield is better in the pressed wheat medium. It was. The required days after moving to the generation room were similar.

  According to the selection method of the present invention, it is possible to select a hon-shimeji mushroom strain that can be harvested in a required number of days after the movement of the development chamber, which is good in a culture medium using a cheaper medium raw material. Artificial cultivation becomes possible.

Claims (5)

  A mutant strain selected from the group consisting of Lyophyllum shimeji La 01-20 (FERM P-16841), Lyophyllum shimeji La 01-27 (FERM P-17455), and Lyophyllum shimeji La 01-45 (FERM P-17457) A hon-shimeji strain that has better fruiting bodies in a culture medium containing corn nuts and hardwood sawdust in a dry matter weight ratio of 2: 1 compared to a culture medium containing oats and hardwood sawdust in a dry matter weight ratio of 2: 1 Honshimeji strain having the ability to form.   A liquid inoculum for artificial cultivation of hon-shimeji mushroom obtained by inoculating and culturing the hon-shimeji mushroom strain according to claim 1 in a liquid medium.   A culture medium for artificial cultivation of hon-shimeji mushroom obtained by inoculating and culturing the hon-shimeji mushroom strain according to claim 1 on a solid culture medium containing corn nuts.   A method for artificially cultivating hon-shimeji mushroom, wherein the strain according to claim 1 is cultured in a culture medium containing corn nuts.   A method for producing hon-shimeji mushroom, comprising culturing the strain according to claim 1 in a culture medium containing corn nuts.