JP2008120701A - Hypoglycemic agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hypoglycemic agent with a compound isolated from specific plant component(s) as an active ingredient, suitable as an antidiabetic having hypoglycemic activity and slight in side effects. <P>SOLUTION: The hypoglycemic agent comprises as the active ingredient a plant belonging to Meliaceae family (e.g. Melia azedarach L., Melia azedarach var. subtripinnata), momordica charantia tea, or extract therefrom. Specifically, the extract from a plant belonging to Meliaceae family is an aqueous or organic solvent extract from the plant's leaves, fruits, branches or bark, and the extract from momordica charantia tea is an aqueous or organic solvent extract from momordica charantia belonging to Cucurbitaceae family. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a blood sugar level-lowering agent suitable as a therapeutic agent for diabetes, and particularly to a blood sugar level-lowering agent containing a plant-derived component and having an excellent blood sugar level lowering effect.

In the process of civilization, diabetics are on the rise due to the emergence of high-quality calorie-quality foods and other lifestyle changes. According to the US CDC report, 22 million diabetics have been Predict that it will occur. Similarly, WHO expects there will be a 4% to 5.4% increase in patients from age 20 and older, and the increase in diabetes is a global problem. Therefore, synthetic drugs that lower blood sugar levels have been developed, and the ultimate therapeutic drug, insulin, has been used by many patients as a desired drug.
In such a situation, a natural plant-derived blood glucose level-lowering substance is required.
On the other hand, the present inventor has found that a plant belonging to the family Sendanidae or an extract thereof has an antitumor activity (Patent Document 1), but it is not yet known that these have a blood glucose level lowering effect.

JP 2004-256426 A

  An object of the present invention is to provide a blood sugar level-lowering agent having a blood sugar level-lowering action and having few side effects, comprising a compound isolated from plant components as an active ingredient.

In the process of studying the activity of plant components, the present invention contains a ginseng plant, bitter gourd tea or an extract thereof containing a component that has an effect of significantly lowering the blood glucose level. It was made based on the knowledge that it can be solved.
That is, the present invention provides a blood glucose level-lowering agent characterized by containing a Sendanid plant, bitter gourd tea or an extract thereof.

  ADVANTAGE OF THE INVENTION According to this invention, it has the outstanding blood glucose level reduction effect and can provide the blood glucose level lowering agent with few side effects.

  The Sendai family used in the present invention is a plant belonging to the family Medeliaceae (Meliaceae), which is mainly an evergreen or deciduous tall tree having a feathery compound leaf at a thick branch tip and having a conical inflorescence. There are also. Of these, it is particularly preferable to use Sendan (Melia Azedarach L. or Melia Azedarach var. Subtripinnata). In the present invention, leaves, stems, branches, bark, and fruits of the family Sendanidae can be used. A root bark can also be used. In the case of using the ginseng plant itself as an active ingredient of the influenza preventive / therapeutic agent, it is preferable to dry these and then finely pulverize them or extract them directly.

In the present invention, the leaves, stems, branches, bark and berries of the gypsidaceae plant, for example, after being dried and pulverized, or in an undried raw state, with water, such as distilled water or ion-exchanged water, or The liquid itself extracted with a hydrophilic or hydrophobic organic solvent or a dried product thereof can be used. Examples of the organic solvent used here include ethyl acetate, carbon tetrachloride, chloroform, dichloromethane, methanol, ethanol, (iso) propyl alcohol, butanol, acetone, or DMSO. Here, the hydrophilic solvent can also be used in a water-containing form. The amount of water or solvent to be used can be set arbitrarily, but it is preferably used in an amount of 1/5 to 10 times, particularly preferably in an equivalent amount. The extraction is preferably performed at a temperature of 60 ° C. or lower, more preferably at room temperature, and particularly with stirring by a mixer or the like.
It is assumed that the active ingredient in the extract of the Sendai family plant has a molecular weight of 10,000 or less.

The water or solvent extract can be used in the liquid state as it is, but it can also be dried and used in a solid form such as powder or granule.
In the present invention, goya tea or an extract thereof can also be used. Here, goya tea can be obtained by drying a bitter gourd plant which is a plant belonging to Cucurbitaceae and extracting it with 1,000 ml of hot water for 1 minute with respect to 4 g of the dry matter. An extract of bitter gourd tea can be prepared in the same manner as that for obtaining an extract of a plant of the family Sendanaceae.

  In addition, in the case of a blood glucose level-lowering agent containing a ginseng plant, bitter gourd tea or an extract thereof, in addition to these, various pharmaceutically acceptable substances for formulation, such as excipients, diluents, disintegrations Agents, binders, coating agents, lubricants, lubricants, lubricants, flavors, sweeteners, solubilizers, and the like can be included as adjuvants. Specific examples include magnesium carbonate, titanium dioxide, lactose, mannitol and other sugars, talc, milk protein, gelatin, starch, cellulose and derivatives thereof, animal and vegetable oils, polyethylene glycol, glycerol and the like.

The blood sugar level-lowering agent of the present invention is preferably administered orally, but is not limited thereto. The blood glucose level-lowering agent of the present invention is preferably used in an amount of about 25 ml per kg body weight for oral administration.
EXAMPLES Next, an Example demonstrates this invention in detail.

Example 1
Extraction Method of Sendan Liquid Sendan (Melia azedarach L.) leaves were collected, weighed in a dry state, and then dried overnight in a dryer at 60 to 65 ° C. The dried leaves were placed in a dipping bag, and 11 times hot water was added, and immersed for 3 hours. Thereafter, the bag was taken out and dehydrated with a commercially available washing machine to obtain an extract. This extract was dispensed into a plastic centrifuge tube, and centrifuged at 6,500 rpm for 30 minutes using a centrifuge, and the resulting supernatant was used as the total crude crude component.
In order to remove toxic components from the total components of the crude fraction obtained by this method, purification was performed using the difference in molecular weight. For purification, a Pellicon 2 mini-holder (MILLIPORE) is equipped with an ultrafiltration membrane (MILLIPORE) with a molecular weight of 10,000, and the total components of the crude fraction are adjusted with the peristaltic pump (MILLIPORE). The liquid was fed while being adjusted to (inlet pressure: 0.5-4 kg / cm ² , outlet pressure: 0.2-1 kg / cm ² ), and the liquid coming out from the permeate side outlet was collected. Further, the collected liquid was used as a test sample (RE110-d) in a test after sterilization by filtration with Sartorius mini-salt (0.22 μm). The molecular weight of the extracted component contained in the subject (RE110-d) is about 10,000 or less.
On the other hand, goya tea was extracted for 1 minute with 1,000 ml of hot water per 4 g of dry matter, sterilized by filtration with a 0.22 μm filter, and this was used as a specimen (goya tea).

  In order to evaluate the diabetes treatment effect of the partially purified extract of Sendan, an evaluation test of the diabetes treatment effect was performed using a type II diabetes model mouse (AKITA / Slc, 5 weeks old, male). Diabetic mice were purchased from Japan SLC. As the administration method, each subject was orally administered daily by 0.5 ml using an oral sonde. In addition, purified water was orally administered to the control group of mice. The blood glucose level was measured twice a week in the fasted state from the day before the measurement, and was measured using a simple blood glucose level measuring device (diameter).

  Table 1 shows blood glucose levels in diabetic mice treated with various subjects. From Table 1, the blood glucose level on Day 0 when three subjects are administered is 309.4 mg / dl in the RE110 component group of Sendang, 277.0 mg / dl in the Goya tea group, and 242.2 mg / dl in the untreated group. It became clear that there was considerable variation among diabetic mice. Using this as a starting point, the blood glucose level on the third day from the start of subject administration was examined, and it was 124.6 mg / dl for RE110, 142.2 mg / dl for Goya tea, and 156.8 mg / dl for the control group. The drop in blood glucose level until the 17th day was clearly higher for Sendang, followed by Goya tea, which revealed that the control group had the lowest value. In order to make this difference more easily understandable, the change rate of the blood glucose level calculated based on the blood glucose level before the start of the examination of the three groups is shown in Table 2 and FIG. From Table 2 and Fig. 1, the change rate of the blood glucose level is the smallest for the entire test schedule, with the RE110-d sendan component being 40.27 on the third day, 58.24 on the seventh day, 62.57 on the tenth day, and the 14th day It was shown to decrease to 79.83% on the 17th day at 64.06. A similar trend was seen in goya tea, with a relative blood glucose level of 51.34 on day 3, 64.98 on day 7, 65.20 on day 10, 127.00 on day 14, and 79.83% on day 17. It has become. On the 14th day, no decrease in blood glucose level was confirmed, but overall, the effect of improving diabetes after Sendan was observed.

Furthermore, in order to make the above results easier to understand, the relative change rates of the blood glucose levels of the RE110 group and goya tea when the blood glucose level of the control group is 100% are shown in Table 3 and FIG. By this comparison, the change in blood glucose level of the RE110 group appears more clearly, and a more significant therapeutic effect is also seen in the goya tea treatment group. As described above, it was confirmed that the effect of diabetes mellitus using the diabetic mouse was the highest in the component of Sendan and reached nearly 40% on the third day after the start of the treatment. Since then, the significant decrease in blood glucose level over the entire period from 7th, 10th, 14th and 17th indicates that the extract of Sendan can be used for the treatment of diabetes in humans.
From the above, it is considered that the blood sugar level-lowering agent of the present invention can greatly contribute to people's health as products of various forms such as various drinks. Furthermore, the blood glucose level-reducing activity based on the blood sugar level-lowering agent of the present invention is not inactivated even by heating at 100 ° C. for 30 minutes. In addition, since goya tea has such a blood glucose level reducing effect at the same time, goya tea or this extract alone can be used as an active ingredient, or it can be used as a hypoglycemic agent in combination with a Sendai plant or its extract. Can do.

Table 1 Blood glucose levels in diabetic mice treated with various subjects

Table 2 Change rate of blood glucose level in diabetic mice in each treatment group (%)

Table 3 Relative change rate of blood glucose level in diabetic mice in each treatment group (%)

It shows the rate of change of blood glucose level calculated based on blood glucose levels before the start of the test of the subject (RE110-d: hot water extract of Sendan), the subject (Goya tea) and the control group (purified water administration). For subjects (RE110-d: hot water extract of Sendan), subjects (goya tea) and control group (purified water administration), blood glucose of RE110 group and goya tea when the blood glucose level of the control group was 100% Indicates the relative rate of change of the value.

Claims (6)

  A blood glucose level-lowering agent characterized by containing a plant of Sendidae, bitter gourd tea or an extract thereof.   The blood sugar level-lowering agent according to claim 1, wherein the extract of the plant of the family Sendanaceae is an aqueous extract of leaves, fruits, branches or bark of the plant of the family Sendanidae.   The blood sugar level-lowering agent according to claim 1, wherein the extract of the plant family is an organic solvent extract of leaves, fruits, branches or bark of the plant family.   The blood sugar level-lowering agent according to claim 1, wherein the extract of bitter gourd tea is an aqueous extract of bitter gourd tea.   The blood sugar level-lowering agent according to claim 1, wherein the extract of bitter gourd tea is an organic solvent extract of bitter gourd tea.   The blood sugar level-lowering agent according to any one of claims 1 to 6, for use as a therapeutic agent for diabetes.

Images