JP2008076208A - Plastic microchip, biochip using it or microanalyzing chip - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a plastic microchip reinforced by an adhesive to be especially enhanced in the adhesive strength of an arbitrary part, and a plastic biochip or a microanalyzing chip obtained thereby. <P>SOLUTION: In the plastic microchip constituted by joining a plastic substrate having a fine flow channel formed on its surface and a plastic film through an adhesive A, the adhesive A or an adhesive B is injected in the groove and/or through-hole provided on the arbitrary part of the plastic substrate and/or the plastic film to join the plastic substrate and the plastic film. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a plastic microchip in which a plastic film for a lid is bonded to a plastic substrate having a fine channel on the surface with an adhesive, and relates to a biochip or a micro analysis chip using the plastic microchip.

  In recent years, biochips, which are devices in which a physiologically active substance is immobilized on a solid substrate, have attracted attention as means for achieving high throughput in drug discovery research and clinical tests. Typical examples of the physiologically active substance to be immobilized include nucleic acids, proteins, antibodies, sugar chains, glycoproteins, aptamers, etc. Nucleic acid microarrays, which are biochips on which nucleic acids are immobilized, are already available in many products. It is on the market. The form of the chip is a form in which various physiologically active substances are spotted and immobilized on a flat substrate, and are mainly used for research analysis in research institutions.

  In recent years, research on miniaturization of chemical reaction, separation, and analysis system using microfabrication technology called micro analysis chip, μTAS (micro total analytical system), or lab-on-chip has become active. Various chemical reactions, particularly physiological reactions, can be performed on the (fine channel). In this system, since a very small amount of sample can be analyzed quickly, it is expected to be commercialized as a next-generation biochip that takes advantage of this feature, particularly as a diagnostic biochip in a medical institution ( Hereinafter, these systems are referred to as micro-analysis chips).

  Currently, these biochips and microanalysis chips are mainly made of glass. In order to produce a micro-analysis chip with a glass substrate, for example, there is a method in which a substrate is coated with a metal or a photoresist resin, and a microchannel pattern is baked, followed by an etching process. However, since glass is not suitable for mass production and is very expensive, plasticization is desired.

    Plastic biochips and microanalysis chips can be manufactured using various plastics by various molding methods such as injection molding. In injection molding, a molten thermoplastic material is introduced into a mold cavity, the cavity is cooled, and the resin is cured, so that a chip substrate can be manufactured efficiently and economically, which is suitable for mass production. However, plastic biochips or microanalytical chips still have many technical drawbacks and have not gained recognition to replace glass. In particular, the biochip or microanalysis chip collectively called microfluidics is an analysis chip characterized by a micro flow path provided inside the chip. There are many drawbacks, and it is still in the research stage. The problem with plastic microfluidics is that it is necessary to attach another plastic plate on the plastic plate that has been processed into a fine flow path, and to cover the fine flow path. The fact that inexpensive, simple, and reliable methods have not yet been found seems to be one of the major factors hindering their practical application.

    In the bonding process in a plastic biochip or microanalysis chip, bonding is mainly performed by a method such as heating, thermocompression bonding with ultrasonic waves or laser, or a method using an organic adhesive. The fusion by heating is likely to cause a deactivation problem of the physiologically active substance due to overheating described later. In the case of an immunoassay using an antigen-antibody reaction, the presence or concentration of a trace amount of an analyte in a sample can be determined by measuring using a method such as thermal lens microscopy. The heating tends to cause deformation in the fine channel and deterioration of the surface properties of the channel surface, which may make measurement difficult. Although heat welding by ultrasonic waves can join surfaces of several millimeters square, it is unsuitable for heat welding of surfaces of several centimeters square, and insufficient welding tends to occur. In the laser irradiation, there is a problem that heating only the central part of the plastic such as the two plastic bonding surfaces is very difficult and the cost of the apparatus is very expensive.

  In addition, when considering application to analysis chips, particularly biochips, various substances such as nucleic acids, proteins, antibodies, sugar chains, glycoproteins, and aptamers are often coated or immobilized on detection sites. Since these physiologically active substances are vulnerable to heating and can be chemically deactivated, the bonding process exposed to high temperatures is unsuitable for the production of biochips and microanalytical chips.

  In addition, if you look at the sticking of plastic products regardless of biochips or microanalysis chips, as a joining method other than the above, a part of the joint surface of the part you are going to join is made with a protrusion, There is a proposal of a method in which a part is bonded to another surface to be joined and the part is bonded by thermal fusion by ultrasonic vibration (see Patent Document 1). However, this method can be used only for relatively small molded products that are not very fine, and the method is not intended for biochips and microanalysis chips having a fine structure.

As a pasting method using an organic solvent, there is means for adhering a solvent by impregnating an organic solvent into a resin as in Patent Document 2, but since the inside of the flow channel is exposed to the organic solvent, If a very small amount of coated material is present, the coating material may be dissolved and removed or decomposed, which is a problem. Another problem is that the bioactive substance tends to be deactivated because it is not a process at room temperature.

Japanese Patent Laid-Open No. 5-16241 JP 2003-118000 A

  The present invention provides a plastic microchip in which the adhesive strength of an arbitrary part is particularly improved by reinforcing with an adhesive, and also provides a plastic biochip or microanalysis chip obtained thereby. It is the purpose.

  As a result of intensive studies to achieve the above-mentioned problems, the present inventors have found that in a plastic microchip formed by joining a plastic substrate having a fine channel on the surface and a plastic film with an adhesive, the plastic substrate and / or A plastic micro with a particularly improved adhesive strength at any part by adopting a method of injecting, bonding, and reinforcing an adhesive inside a groove and / or through-hole provided in an arbitrary part of the plastic film I found that I can get a chip. Moreover, it was confirmed that a plastic biochip and a plastic microanalysis chip processed using the same could be realized, and the present invention was achieved.

That is, the present invention
(1) In a plastic microchip formed by joining a plastic substrate having a fine channel on the surface and a plastic film via an adhesive A, a groove provided in an arbitrary portion of the plastic substrate and / or plastic film And / or a plastic microchip which is reinforced by injecting and bonding the adhesive A or the adhesive B into the through-hole,
(2) The plastic microchip according to (1), wherein the plastic substrate and the plastic film have a 90 ° peel adhesive strength at room temperature of 2 N / 25 mm or more,
(3) The plastic microchip according to (1) or (2), wherein the plastic film has a thickness of 0.01 to 1 mm,
(4) The plastic microchip according to any one of (1) to (3), wherein the plastic is any one of acrylic resin, saturated cyclic polyolefin, polymethyl methacrylate, polycarbonate, polystyrene, and polyethylene terephthalate,
(5) The plastic microchip according to any one of (1) to (4), wherein the plastic film has a flexural modulus of 500 to 15000 MPa.
(6) The plastic microchip according to any one of (1) to (5), wherein the adhesive A has a thickness of 0.1 to 20 μm.
(7) The plastic microchip according to any one of (1) to (6), wherein the adhesive A and the adhesive B contain an acrylic resin,
(8) The plastic microchip according to any one of (1) to (7), wherein the adhesive A and the adhesive B are ultraviolet curable adhesives,
(9) The plastic microchip according to any one of (1) to (8), wherein the joint portion is not damaged when water is passed through the fine channel portion at a pressure of 300 kPa.
(10) In the plastic microchip according to any one of (1) to (9), a biochip that is at least one selected from a nucleic acid chip, a protein chip, an antibody chip, an aptamer chip, and a glycoprotein chip, or various types Micro analysis chip for chemical analysis,
It is.

According to the present invention, when a plastic biochip or microanalysis chip having a fine flow path is bonded, it can be quickly bonded with sufficient bonding strength and without blocking the fine flow path. In particular, it is possible to selectively improve the strength where a bonding strength is required, and this is a particularly effective technique for manufacturing plastic biochips and microanalysis chips.

Hereinafter, embodiments of the present invention will be described in detail.
The present invention provides a plastic microchip in which a plastic substrate having a fine channel on the surface and a plastic film are bonded with an adhesive to particularly improve the adhesive strength of an arbitrary portion, and uses the same. Biochip or microanalysis chip.

Examples of the plastic used for the plastic substrate or plastic film used in the present invention include high-density polyethylene, low-density polyethylene, polypropylene, polystyrene, various cyclic polyolefins, polymethyl methacrylate, polynorbornene, polyphenylene oxide, polyethylene terephthalate, Like polyethylene naphthalate, polycarbonate, polyamide, polyimide, polyester, semi-cured phenol resin, semi-cured epoxy resin, Teflon (registered trademark), polyvinylidene chloride, polyvinyl chloride, and other various thermoplastics, A polymer substance having a melting point and Tg is shown, but there is no particular limitation on the physical properties such as the type, degree of polymerization, melting point, Tg, and elastic modulus. Further, there is no problem even if only one type of material is used, but it is more preferable that a plurality of materials are combined in an appropriate place.
Among these, acrylic resin, saturated cyclic polyolefin, polymethyl methacrylate, polycarbonate, polystyrene, or polyethylene terephthalate is preferable.

  The present invention is mainly based on the premise that plastic members are joined, but it is considered that this technique can be applied not only to plastics but also to plastics and non-plastics. Non-plastic here refers to copper, aluminum, iron, silicon, nickel and other various metals and alloys thereof, metal oxides such as silica, alumina, zirconia, titania, mixtures thereof, glass materials thereof, Various ceramics such as silicon carbide and boron nitride, linear wiring and foil-shaped wiring and various sensors made of these ceramics, various sensors, and other materials that do not fall under plastics, such as paper and wood, are targeted. Alternatively, fully cured phenolic resins and fully cured epoxy resins also fall into the category.

  The main object of the present invention is a plastic microchip or microanalysis chip having a fine flow path. The fine flow path has a width of 1000 μm or less and a depth of 500 μm or less. It is preferable that there is a capped groove formed on the premise of flowing a liquid substance such as water or an organic solvent, that is, a flow path. There is no limitation on the length of the fine channel and the ratio to the entire surface. There is no limitation on the surface roughness and surface energy of the inner wall of the fine channel.

Although the thickness of the plastic film used for this invention is not specifically limited, It is preferable that it is 0.01-1 mm. When the thickness of the plastic film exceeds 1 mm, the plastic film does not sufficiently follow the unevenness of the plastic substrate when the plastic member is bonded, and the plastic film may not adhere to the adhesive or may be peeled off. If the thickness is less than 0.01 mm, the plastic film itself may be destroyed when a liquid material such as water is flowed into the fine flow path portion. There is a risk that it cannot be sealed.

Although the bending elastic modulus of the plastic film used for this invention is not specifically limited, It is preferable that it is 500-15000 MPa. If the flexural modulus of the plastic film exceeds 15000 MPa, the plastic film may not sufficiently follow the unevenness of the plastic substrate when the plastic member is bonded, and the plastic film may not adhere to the adhesive or may be peeled off. is there. If it is less than 500 MPa, the plastic film is likely to wrinkle at the time of bonding, and the flow path may not be sufficiently sealed. The flexural modulus of the plastic film can be measured by, for example, the test method ASTM D790.

The adhesive A and the adhesive B used in the present invention are not particularly limited as long as they are adhesives that satisfactorily bond the target plastic. Further, the adhesive A and the adhesive B may be the same or different. As an adhesive, an acrylic adhesive, an epoxy adhesive, a urethane adhesive, a polyester adhesive, an ultraviolet curable adhesive or a thermosetting adhesive, or a hot melt adhesive is generally used. Any adhesive used can be used. However, it is natural that a certain degree of adhesion to the resin to be bonded is necessary as a condition for selecting the adhesive. In addition, since it can be bonded at a low temperature and has high adhesive strength, an ultraviolet curable adhesive, particularly an acrylic resin-based ultraviolet curable resin is preferably used. Furthermore, there is no particular limitation on the surface treatment of the adhesion surface before adhesion, for example, plasma treatment, ultraviolet treatment, corona discharge treatment, excimer treatment, various primer treatments, for example, coupling agent treatment. However, for the curing system, the curing conditions corresponding to the adhesive are selected.

  Regarding the viscosity of the adhesive used in the present invention, the viscosity at 25 ° C. is preferably in the range of 0.5 to 100 Pa · s, more preferably 1 to 50 Pa · s. If it is less than the lower limit value, the resin tends to flow into the fine channel when the adhesive is applied to the plastic substrate and bonded, and the channel is likely to be blocked. On the other hand, if the upper limit is exceeded, the adhesive does not have fluidity, so that the unevenness of the plastic substrate cannot be sufficiently filled. In addition, since entrainment of bubbles and the like is likely to occur, problems such as non-uniform adhesion strength near the flow path and deterioration of aesthetics due to entrainment of bubbles are likely to occur. Regarding the viscosity of the adhesive, it is necessary to select an optimum one depending on the structure and surface state of the adhesive. The viscosity of the adhesive can be determined with a general viscometer. If a specific example is given, a viscosity can be calculated | required with the Brookfield BH type | mold or BL type | mold rotational viscometer.

  The method for applying the adhesive A is not particularly limited, but a bar coater, a spin coater, a spray coater, a roll coater, a gravure coater, a knife coater, and other various coating apparatuses can be used. For methods that do not stuff the adhesive into the fine channel, either a method of coating after covering only the fine channel part with a mask, or a method of uniformly coating only a plastic plate (so-called lid part) that does not process the fine channel Examples of the method include, but are not particularly limited to, a method in which a structure that has been coated with an adhesive on another support and then subjected to micro-channel processing is pressed to transfer the adhesive only to a necessary portion. However, the method disclosed in Japanese Patent Application No. 2006-66725 is most suitable.

In the present invention, the shape of the groove and / or the through hole provided in the plastic substrate and / or the plastic film can be proposed as shown in FIGS. There are no particular restrictions on the size and depth, but it is necessary to design an optimum one depending on the size of the chip, the shape of the flow path, the arrangement, and the like.
The method for injecting the adhesive is not particularly limited, but a quantitative injection method using a dispenser or the like can be used. The adhesive B may be completely filled in the through holes or grooves, or there may be voids.

By injecting and bonding an adhesive into the groove and / or through hole provided in any part of the plastic substrate and / or plastic film, stress from the outside is applied to the adhesive part filled in the groove or through hole. It is presumed that the adhesion strength is improved as a result.

  The thickness of the adhesive A is preferably 0.1 to 20 μm. If the thickness of the adhesive is less than the lower limit, not only a sufficient adhesive strength cannot be obtained, but bubbles are likely to enter during bonding, which is not preferable. On the other hand, exceeding the upper limit is not preferable because the resin easily flows into the fine flow path when the adhesive is applied to and bonded to the plastic substrate, and the flow path is easily blocked.

In the microchip of this patent, it is possible to incorporate a mechanism such as a one-way valve, a switching valve mechanism, a filter, various functional group-containing beads, and a dam structure.
The one-way valve is a valve for flowing a liquid only in one direction, and indicates a mechanism that can prevent back flow of fluid by incorporating it. Also called check valve.
The switching valve mechanism is a method for mechanically controlling whether or not liquid is allowed to flow through a flow path. Fluid is allowed to flow from a plurality of flow paths to only one flow path, or fluid is allowed to flow only to one flow path. The choice of not is also applicable.
The filter indicates a network structure capable of sorting solids and liquids, or sorting particles having a fine particle size and particles having a rough particle size.
The various functional group-bearing beads indicate beads smaller than the fine channel in which the functional groups are embedded, and have a certain functional group that expects a chemical reaction on the surface thereof. When using beads, the dam structure is often constructed so that the beads do not flow through the flow path.

  In the microchip of the present invention, an electric sensor can be incorporated. A method of confirming the result of the reaction by electric conductivity is very commonly performed. It can also be used as an electrode for fluid control such as electrophoresis. There are no particular limitations on the electrode arrangement structure and the purpose of use, but it is advantageous to incorporate a metal foil or metal plating having a thickness of 0.01 to 50 μm, both in terms of manufacturing process, measurement and adult fish.

  In the present invention, it is preferable that there is no undesignated blockage in the fine channel portion, and that the joint portion is not damaged at all even if water with a pressure of 300 kPa flows through the fine channel portion. In a biochip or microanalysis chip, liquid or gas is allowed to flow through the microchannel, but unlike the intention designed when these fluids are joined to the chip, the microchannel must not be blocked by an adhesive. Of course, this is because it is necessary that the liquid and gas components are sufficiently sealed from practical use so as not to leak from the fine channel portion. Using a plunger pump, etc., flow 300 kPa of water through the flow path of the biochip or microchemical chip, and observe whether the water passes through the fine flow path part as designed or whether the fine flow path part breaks and water does not leak. This can be confirmed by observation.

  In the present invention, 90 ° at room temperature of a portion reinforced by injecting adhesive A or B into a groove and / or through-hole provided in an arbitrary portion of a plastic substrate and / or a plastic film and joining them. The peel adhesion strength is preferably at least 2 N / 25 mm. This is because, when the 90 ° peel adhesive strength at room temperature is less than 2 N / 25 mm, the plastics are easily peeled off by an external force, for example. The 90 ° peel adhesive strength can be measured by the test method JIS-K-6854-1.

  The microchip using the plastic bonding method of the present invention is characterized in that it can be used as a plastic biochip or a microanalysis chip which is firmly bonded to a relatively large area without contamination and has good performance. In particular, it can be suitably used for products subjected to fine processing such as microfluidics. In particular, a product group in which a physiologically active substance such as a nucleic acid chip, protein chip, antibody chip, aptamer chip, glycoprotein chip, etc. is immobilized on the surface or inside of the chip can be mentioned. Or the analysis chip | tip for chemical analysis is mentioned.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

(Example 1)
Using an acrylic resin as a raw material, a molded product shown in FIG. 5 was obtained by molding and cutting. This molded product has a through hole 1 (diameter 2 mm), a through hole 2 (diameter 1 mm), and a fine channel 3 (length 25 mm, cross-sectional shape is rectangular, depth 100 μm, width 200 μm). A film obtained by extrusion molding using an acrylic resin as a raw material was cut into the shape shown in FIG. In addition, the bending elastic modulus of this resin film is 2940 MPa. An ultraviolet curable adhesive A (manufactured by ThreeBond Co., Ltd., 3003, acrylic resin, viscosity at 25 ° C .: 1.5 Pa · s) is applied to one side by a bar coater on the processed surface side of the molded product of FIG. However, the adhesive was not attached to the through holes 1 and 2 and the fine flow path 3 by applying a masking tape previously cut into the shape and masking. After removing the masking tapes of the through holes 1 and 2 and the fine flow path 3, the molded product and the film were bonded together while removing bubbles. Thereafter, an ultraviolet curable adhesive B (manufactured by ThreeBond Co., Ltd. 3027, acrylic resin, viscosity at 25 ° C .: 2.0 Pa · s) was injected into the through-hole 2 using a dispenser. Thereafter, the adhesive was cured by an ultraviolet curing device. The amount of ultraviolet irradiation was 3000 mJ / cm ² . The thickness of the adhesive A was 10 μm as a result of observation by cross-sectional observation of the obtained bonded sample. The evaluation results are shown in Table 1.

(Example 2)
Using an acrylic resin as a raw material, a molded product shown in FIG. 6 was obtained by molding and cutting. The molded product has a through-hole 1 (diameter 2 mm), a fine channel 3 (length 25 mm, a cross-sectional shape is rectangular, a depth 100 μm, a width 200 μm), and a circular groove 4 (diameter 1 mm, depth 500 μm). An ultraviolet curable adhesive A (manufactured by ThreeBond Co., Ltd., 3003, acrylic resin, viscosity at 25 ° C .: 1.5 Pa · s) is applied to the processed surface side of the molded product of FIG. However, the adhesive was prevented from adhering to the through hole 1, the fine flow path 3, and the circular groove 4 by applying a masking tape previously cut into the shape and performing masking. After removing the masking tape of the through-hole 1, the fine flow path 3, and the circular groove 4, an ultraviolet curable adhesive B (manufactured by ThreeBond Co., Ltd. 3027, acrylic resin system, viscosity at 25 ° C .: 2.0 Pa is applied to the circular groove 4. • s) was injected with a dispenser. Then, this molded product and the acrylic film of FIG. 8 were bonded together while removing bubbles. Thereafter, the adhesive was cured under the same conditions as in Example 1 using an ultraviolet curing device. The thickness of the adhesive A was 7 μm as a result of observation by cross-sectional observation of the obtained bonded sample. The evaluation results are shown in Table 1.

(Example 3)
Using an acrylic resin as a raw material, a molded product shown in FIG. 7 was obtained by molding and cutting. This molded product has a through-hole 1 (diameter 2 mm), a fine flow path 3 (length 25 mm, cross-sectional shape is rectangular, depth 100 μm, width 200 μm), rectangular groove 5 (length 38 mm, cross-sectional shape is rectangular, depth) 500 μm, width 1 mm). An ultraviolet curable adhesive A (3003 manufactured by Three Bond Co., Ltd., acrylic resin system, viscosity at 25 ° C .: 1.5 Pa · s) is applied to one side by a bar coater on the processed surface side of the molded product of FIG. However, the adhesive was not attached to the through hole 1, the fine flow path 3, and the rectangular groove 5 by applying a masking tape previously cut into the shape and masking. After removing the masking tape of the through hole 1, the fine flow path 3, and the rectangular groove 5, an ultraviolet curable adhesive B (manufactured by ThreeBond Co., Ltd. 3027, acrylic resin, viscosity at 25 ° C .: 2.0 Pa in the circular groove 5. • s) was injected with a dispenser. Then, this molded product and the acrylic film of FIG. 8 were bonded together while removing bubbles. Thereafter, the adhesive was cured under the same conditions as in Example 1 using an ultraviolet curing device. The thickness of the adhesive A was 1 μm as a result of observation by cross-sectional observation of the obtained bonded sample. The evaluation results are shown in Table 1.

Example 4
The molded product of FIG. 5 made of acrylic resin and the film of FIG. 8 made of acrylic resin were joined. An ultraviolet curable adhesive A (3003 manufactured by ThreeBond Co., Ltd., acrylic resin system, viscosity at 25 ° C .: 1.5 Pa · s) is applied on one surface using a bar coater 7 on the rubber roll 6 shown in FIG. As shown in FIG. 11, the ultraviolet curable adhesive A applied to the rubber roll 6 was transferred to the processed surface side of the molded product of FIG. Then, as shown in FIG. 12, this molded product and the film 9 were laminated. Thereafter, an ultraviolet curable adhesive B (manufactured by ThreeBond Co., Ltd. 3027, acrylic resin, viscosity at 25 ° C .: 2.0 Pa · s) was injected into the through-hole 2 using a dispenser. Thereafter, the adhesive was cured under the same conditions as in Example 1 using an ultraviolet curing device. The thickness of the adhesive was 10 μm as a result of observation by cross-sectional observation of the obtained bonded sample. The evaluation results are shown in Table 1.

(Comparative Example 1)
Using an acrylic resin as a raw material, a molded product shown in FIG. 9 was obtained by molding and cutting. This molded product has a through-hole 1 (diameter 2 mm) and a fine channel 3 (length 25 mm, cross-sectional shape is rectangular, depth 100 μm, width 200 μm). An ultraviolet curable adhesive A (manufactured by ThreeBond Co., Ltd., 3003, acrylic resin, viscosity at 25 ° C .: 1.5 Pa · s) is applied to the processed surface side of the molded product of FIG. However, the adhesive was not attached to the through hole 1 and the fine flow path 3 by applying a masking tape previously cut into a shape and masking. After removing the masking tape of the through-hole 1 and the fine flow path 3, this molded product and the acrylic film of FIG. 8 were bonded together while removing bubbles. Thereafter, the adhesive was cured under the same conditions as in Example 1 using an ultraviolet curing device. The thickness of the adhesive A was 10 μm as a result of observation by cross-sectional observation of the obtained bonded sample. The evaluation results are shown in Table 1.

Evaluation Method (1) Observation of Adhesive Layer The bonded adhesive layer was visually or optically observed, and the number of bubbles having a diameter of 100 μm or more was counted. It was judged that a bubble with more than 5 bubbles was unsuitable and a bubble with less than 5 bubbles was suitable.
(2) Strength experiment of fine flow path Using a plunger pump, water is allowed to flow through the fine flow path portion with the water pressure set to 300 kPa while observing the fine flow path portion with an optical microscope. Confirm by observation that there is no leakage of water from the flow path or blockage of the flow path.
(3) Evaluation by thermal lens microscopy The sample was poured into the fine flow path of the joined plastic microchip, and the presence or concentration of the analyte in the sample was evaluated by thermal lens microscopy. Details of the thermal lens microscope are described in, for example, Japanese Patent Laid-Open No. 2000-356611. In this evaluation, an argon laser was used as an excitation light source, a helium neon laser was used as a detection light source, and the spot diameter was 1 μm. The sample flowing in the flow path was water, and whether or not the excitation and detection lasers were focused was used as a criterion for this evaluation. There was no problem when the laser focused and there was no problem in measurement, and there was a problem when measurement was difficult because the laser was not focused.
(4) Adhesive strength evaluation of chip edge part The corner | angular part of the joined plastic microchip was flicked with the finger, and the peeling condition of the film in the chip edge part was observed. Evaluation was performed with 10 microchips, and the number of peeled sheets was measured.

It is a schematic diagram of the plane and cross section which show the example of a through-hole. It is a schematic diagram of a plane and a cross section showing an example of a circular groove. It is a schematic diagram of the plane and cross section which show the example of a rectangular penetration channel. It is a schematic diagram of a plane and a cross section showing an example of a rectangular groove. It is a schematic diagram of a plane and a section showing a finely processed molded product used in Examples 1 and 4. It is a schematic diagram of a plane and a section showing a finely processed molded product used in Example 2. FIG. 6 is a schematic diagram of a plane and a cross section showing a microfabricated molded product used in Example 3. It is a schematic diagram of the plane and cross section of the acrylic film used for the Example and the comparative example. It is a schematic diagram of a plane and a section showing a finely processed molded product used in Comparative Example 1. It is a schematic diagram of the process of apply | coating the ultraviolet curable adhesive A used for Example 4 to a rubber roll. It is a schematic diagram of the process of transcribe | transferring the ultraviolet curable adhesive apply | coated to the rubber roll used for Example 4 to the processed surface of a molded article. It is a schematic diagram of the process of laminating the molded article and the film which apply | coated the ultraviolet curable adhesive used for the Example and the comparative example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Through hole 2 Through hole 3 Micro flow path 4 Circular groove 5 Rectangular groove 6 Rubber roll 7 Bar coater 8 Roll 9 Film 10 Roll 11 Roll

Claims (10)

In a plastic microchip formed by joining a plastic substrate having a fine channel on the surface and a plastic film via an adhesive A, grooves and / or provided in any part of the plastic substrate and / or plastic film A plastic microchip which is reinforced by injecting and bonding adhesive A or adhesive B into a through hole. 2. The plastic microchip according to claim 1, wherein a 90 ° peel adhesive strength at room temperature between the plastic substrate and the plastic film is 2 N / 25 mm or more. The plastic microchip according to claim 1 or 2, wherein the plastic film has a thickness of 0.01 to 1 mm. The plastic microchip according to any one of claims 1 to 3, wherein the plastic is any one of acrylic resin, saturated cyclic polyolefin, polymethyl methacrylate, polycarbonate, polystyrene, and polyethylene terephthalate. The plastic microchip according to any one of claims 1 to 4, wherein the plastic film has a flexural modulus of 500 to 15000 MPa. The plastic microchip according to claim 1, wherein the adhesive A has a thickness of 0.1 to 20 μm. The plastic microchip according to claim 1, wherein the adhesive A and the adhesive B contain an acrylic resin. The plastic microchip according to claim 1, wherein the adhesive A and the adhesive B are ultraviolet curable adhesives. The plastic microchip according to any one of claims 1 to 8, wherein the joint portion is not damaged when water is passed through the fine channel portion at a pressure of 300 kPa. 10. The plastic microchip according to claim 1, wherein the biochip is at least one selected from a nucleic acid chip, a protein chip, an antibody chip, an aptamer chip, and a glycoprotein chip, or various chemical analysis microchips. Analysis chip.

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