JP2008064463A - Structure analyzing method of soft material due to beam irradiation and soft material holding device used therein - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a structure analyzing method of soft material due to beam irradiation capable of simply fixing a sample of the soft material subjected to beam irradiation to a measuring instrument so as not to move the same and simply keeping a state that a solution is allowed to uniformly flow to the sample, and a soft material holding device. <P>SOLUTION: The skin 1 of the soft material is inserted in the sample housing hole 23 of a plate shaped sample housing member 22 having solution permeability, the sample housing member 22 is held in the holding hole 27a of holding metal fittings 25, both sides of the holding metal fittings 25 as covered with a thin-walled cover material 38 and a pair of case metal fittings 41 and 42 are fixed to the holding metal fittings 25 to fix the skin 1 in an immovable state in the sample housing hole 23. Further, the solution is allowed to flow through the holding hole in a predetermined ratio by a Perista pump device 55 to allow the solution to uniformly flow to the skin 1 through the sample housing member 22. The skin 1 is irradiated with X rays through a beam passing hole 47 to perform measurement by an X-ray diffraction device. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a soft material using a measuring device using a beam structure such as X-ray diffraction / scattering or infrared absorption for the structure of a soft material such as a fiber structure such as cellulose, a film structure such as skin, or a three-dimensional structure such as food. The present invention relates to a structural analysis method and a soft material holding device used therefor.

  As a soft material of this kind, for example, intercellular lipids in the stratum corneum of animal skin play an important role in the barrier function of the skin, and it has been a challenge to clarify the mechanism at the molecular level. It has become. In this case, structural analysis using an X-ray diffractometer is employed, but there is a problem that individual differences cannot be avoided in experiments using the stratum corneum collected from the skin of animals. For example, when glycerol is used as a solution to affect the stratum corneum by observing structural changes in intercellular lipids, the difference in structure observed by X-ray diffraction is due to the contribution of glycerol, or It is difficult to distinguish between individual differences due to large variations in structure among individuals, and it is even more difficult to detect structural changes when the structural change due to glycerol is not large. In order to solve such difficult problems, it is necessary to fix the sample of the skin stratum corneum so that it does not move. It is necessary to be in the state.

Conventionally, for example, a method of inserting a sample using a glass capillary as shown in Patent Document 1 has been used. However, it is difficult to fix the sample so that it does not move with a glass capillary. Furthermore, since the solution cannot be uniformly supplied into the glass capillary, it was necessary to individually analyze the data by inserting the sample without the solution and the sample with the solution into the capillary. . For this reason, measurement without individual differences in the samples was impossible.
JP 2002-277356 A

  The present invention is intended to solve the above-described problems. A soft material sample to be irradiated with a beam can be easily fixed so as not to move to a measuring apparatus, and the solution can be flowed uniformly on the sample. It is an object of the present invention to provide a structure analysis method for a soft material by beam irradiation and a soft material holding device used therefor, which can perform measurement while maintaining a simple state.

  In order to achieve the above object, a structural feature of the present invention is that a sample of a soft material is inserted into a sample storage hole of a plate-shaped sample storage member having a sample storage hole and has a holding hole. At the same time, the sample storage member is inserted into the holding holes of the plate-shaped holding metal fittings having the solution flow holes connected to the holding holes at two locations on the outer periphery, and the sample holding holes are made liquid-tight by covering the both sides of the holding metal fittings with thin coating materials. The holding metal fittings are clamped from both sides with a pair of case metal fittings provided with a beam passage hole arranged so as to surround the sample accommodation hole, and fixed to the holding metal fittings by tightening means. The measurement is performed by circulating the solution through the solution flow hole into the sample accommodation hole by the flow means and irradiating the sample with the measurement beam through the beam passage hole by the beam measuring device. In addition, as a beam measuring device, those using X-ray diffraction / scattering, neutron diffraction / scattering, infrared absorption, ultraviolet-visible absorption spectrum, circular dichroism, Raman spectrum, electron spin resonance, nuclear magnetic resonance, fluorescence spectroscopy, etc. is there. Soft materials include cellulose, keratin, collagen, polymer, fiber, etc. in the fiber structure, skin, nerve, intestinal wall, etc. in the film structure, starch, polysaccharide, powder as the three-dimensional structure , Cells, living tissues, foods, etc.

  In the present invention, a soft material sample is inserted into a sample accommodation hole of a plate-like sample accommodation member having a solution permeability, the sample accommodation member is held in a holding hole of a holding fitting, and both sides of the holding fitting are covered with a thin wall In addition, a pair of case fittings are clamped from both sides by a pair of case fittings, and are clamped by fastening means to be fixed to the holding fixture, so that the sample does not move into the sample receiving hole. It is fixed with. Furthermore, the solution flows through the solution circulation hole into the holding hole at a predetermined rate by the solution circulation means, so that the solution flows through the sample-containing member having the solution permeability, and the soft sample stored in the sample-receiving hole is made uniform. The solution flows through. In this state, the measurement can be performed in a stable state by irradiating the sample with a measurement beam through the beam passage hole by the beam measurement device. As a result, in the present invention, even when a minute change such as a structural change of the intercellular lipids in the skin is observed while flowing the solution, the observed difference in structure is due to the contribution of the solution or due to individual differences. It can be distinguished whether it is a thing.

  In the present invention, a filter paper can be used as the sample storage member. As a result, the filter paper is crushed in the sample-receiving hole, and the soft sample is reliably supported so as not to move.

  Moreover, in this invention, the difference of the measurement result by the beam measuring device in the state which does not introduce | transduce a solution in a sample accommodation hole, and the state which introduce | transduced can be obtained. Thereby, even when the structural change due to the solution is not large, the structural change can be analyzed in detail by taking the difference between the measurement results in the state where the solution is not circulated and the state where the solution is circulated.

  Another feature of the present invention is a plate-like sample accommodation member having a solution permeability provided with a sample accommodation hole into which a sample of a soft material is inserted as a soft material holding device used for structural analysis of the soft material, A plate-shaped holding metal fitting having a holding hole for holding the sample holding member and having a solution flow hole connected to the holding hole at two positions on the outer periphery, and a sample holding hole arranged in close contact with both sides of the holding metal fitting A pair of thin coating materials that are coated in a liquid-tight state and a beam passage hole that is disposed on both sides of the holding fixture via the coating material and surrounds the sample receiving hole are provided, and the holding fixture is sandwiched and tightened. A pair of case fittings fastened and fixed to the holding fitting by the attaching means and a solution circulation means for circulating the solution through the solution circulation hole and into the sample accommodation hole are provided. By this soft material holding device, the sample is securely fixed in a state where it does not move into the sample accommodation hole, and the solution is uniformly supplied to the soft sample accommodated in the sample accommodation hole by the solution circulation means.

  According to the present invention, a soft material sample is inserted into a sample accommodation hole of a plate-like sample accommodation member having a solution permeability, and is fixed in a sample accommodation hole by a holding metal fitting, a covering material, and a pair of case metal fittings. In this state, even when a minute structural change is observed while flowing the solution through the sample, it is possible to distinguish whether the observed difference in structure is due to the contribution of the solution or due to individual differences. Moreover, it is preferable to use a filter paper as the sample storage member, and the sample storage member is simply and reliably supported so that the sample does not move by the filter paper. Moreover, in this invention, the difference of the measurement result by the beam measuring device in the state which did not distribute | circulate the solution in the sample accommodation hole, and the state distribute | circulated can be obtained. Thereby, even when the structural change due to the solution is not large, the structural change can be analyzed in detail by taking the difference between the measurement results in the state where the solution is not circulated and the state where the solution is circulated.

  Embodiments of the present invention will be described below. 1 and 2 are a plan view and a partially cutaway front view of a soft material holding device used for structural analysis of skin, which is a soft material using an X-ray diffractometer as an embodiment. 3 is an enlarged partially cutaway front view of the apparatus main body of the soft material holding apparatus. The soft material holding device 10 includes a case portion 11, a device main body 21 attached thereto, and a peristaltic pump device 55 that is a solution circulation means.

  The case portion 11 is formed by combining rectangular metal plates, and is a rectangular flat substrate portion 12 and a pair of side plates fixed vertically in the same direction on both short sides 12a and one long side 12b. A portion 13 and a horizontal plate portion 14 are provided. The substrate portion 12 has a transmission hole 12d at the center through which X-rays that penetrate the plate pass. Each side plate 13 is provided with a through screw hole 13a on the side of the substrate portion 12 at the center in the longitudinal direction, and an adjustment screw 15 is screwed into each of the through screw holes 13a. The adjustment screw 15 presses a pair of positioning pieces 16 placed on the substrate portion 12 on the distal end side to position the apparatus main body 21 sandwiched between the positioning pieces 16. Further, at two locations on both ends of the other long side 12c of the substrate part 12, block-like connecting parts 17 are arranged so as to form a central angle of 90 ° toward the center of the substrate part 12, respectively. It is fixed to the part 12. Each connecting portion 17 is provided with a connecting small hole 17 a that is a through hole extending horizontally toward the center of the substrate portion 12. A mounting rod 18 is fixed and extends vertically at the center of the outer surface of the horizontal plate portion 14, and a rectangular mounting plate 19 is fixed vertically to the mounting rod 18 at the tip of the mounting rod 18. At the four corners of the mounting plate 19, mounting holes 19a are provided.

  The apparatus main body 21 has a plate-like sample accommodation member 22 having solution permeability provided with a sample accommodation hole 23 into which a soft sample is inserted at the center, and a holding hole 27a in which the sample accommodation member 22 is accommodated in the center. The plate-like holding metal fitting 25 having solution flow holes 29 and 36 connected to the holding hole 27a at two places on the outer periphery, and the sample containing hole 23 covered in a liquid-tight state are disposed in close contact with both sides of the holding metal fitting 25. A pair of thin-walled covering members 38 and a pair of case fittings 41 and 42 in which the holding fitting 25 is disposed on both sides via the covering member 38 are provided.

  The sample accommodation member 22 is a disc-like disk made of filter paper having solution permeability, and is provided with a sample accommodation hole 23 which is a circular small hole into which a skin made of a soft material is inserted in the center. In addition to the filter paper, it is also possible to use a porous inorganic material having solution permeability, a thick cloth such as wool or Rasha, a non-woven fabric, or the like. The holding metal fitting 25 is composed of a central metal fitting portion 26 made of a thin plate and an annular outer annular fitting portion 31 surrounding the central fitting 26. The central metal part 26 is integrally provided with a flat circular central part 27 and an annular ring part 28 projecting on both sides of the central part 27 along the outer peripheral edge of the central part 27. A holding hole 27 a into which the sample storage member 22 is inserted is provided at the center of the central portion 27. Furthermore, a pair of solution flow holes 29 connected to the holding holes 27a are formed at two locations 90 ° apart on the outer peripheral side of the central metal fitting 26.

  The outer annular metal part 31 is provided with a pair of upper and lower annular parts 32 and 33 that can be separated from each other. The upper and lower portions 32 and 33 are provided with annular recesses 34 and 35 cut out in a rectangular cross section along the entire circumference at the boundary between the inner peripheral surface and the opposing surfaces. In addition, a pair of solution circulation holes 36 connected to the pair of solution circulation holes 29 are formed across the upper and lower parts 32 and 33 at two positions 90 ° apart on the outer peripheral side of the upper and lower parts 32 and 33. Furthermore, the outer annular metal part 31 is provided with mounting holes 37 that penetrate the plate at eight equally spaced positions in the circumferential direction near the outer periphery. The outer peripheral side of the central metal part 26 is sandwiched between the upper and lower parts 32 and 33, the ring part 28 is sandwiched between the concave parts 34 and 35, and the outer annular metal part 31 and the central metal part 26 are integrated. The thin covering material 38 is disposed in close contact with both sides of the central portion 27 of the holding metal fitting 25 to cover the holding hole 27a in a liquid-tight state, and is flexible and has insulation and corrosion resistance, for example, polyimide A membrane is used.

  The pair of upper and lower case metal parts 41 and 42 are provided with center convex parts 43 and 44 projecting in a circular shape at the center part on the overlapping surface with respect to the holding metal part 25. The central protrusions 43 and 44 have an outer diameter slightly smaller than the inner diameters of the upper and lower parts 32 and 33 and can be inserted into the central space of the upper and lower parts 32 and 33. In the vicinity of the outer periphery of the flat surface of the central protrusions 43 and 44, annular recesses 43a and 44a are coaxially provided, and O-rings 45 and 46 are mounted in the recesses 43a and 44a. The upper case fitting 42 is provided with a beam passage hole 47 into which X-rays inclined in a conical shape are incident so as to have a small diameter on the central convex portion 43 side at the center. The opening diameter of the beam passage hole 47 on the central convex portion 43 side is larger than the diameter of the sample accommodation hole 23. Further, the lower case fitting 43 is provided with a beam passage hole 48 through which X-rays, which are straight circular through holes, are transmitted at the center. The diameter of the beam passage hole 48 is substantially the same as the small diameter portion of the beam passage hole 47. The case metal fittings 41 and 42 are provided with attachment holes 49 penetrating the plate at eight positions equally spaced in the circumferential direction corresponding to the attachment holes 37 of the outer annular metal fitting part 31.

  The apparatus main body 21 inserts the skin 1 which is a soft sample into the sample accommodation hole 23 of the sample accommodation member 22, inserts the sample accommodation member 22 into the holding hole 29 of the central fitting portion 26 of the holding fitting 25, and the central fitting portion 26. The outer annular metal fitting 31 is sandwiched between the two surfaces. Upper and lower case metal fittings in which a covering material 38 made of a polyimide film is covered on both surfaces of the central portion 27 of the holding metal fitting 25, and O-rings 45 and 46 are mounted on the recesses 43 a and 44 a on both surface sides of the holding metal fitting 25. 41 and 42 are clamped with the center convex portions 43 and 44 side aligned. Then, the bolts and nuts 51 are tightened and fixed through the attachment holes 49 of the case fittings 41 and 42 and the attachment holes 37 of the outer annular fitting 31. Thereby, the skin 1 is supported simply and reliably so as not to move due to the flaking of the filter paper by using the filter paper as the sample storage member 22.

  Furthermore, the apparatus main body 21 is obtained by inserting the solution supply / discharge pipe 56 of the peristaltic pump device 55 as the solution circulation means into the connection small hole 17a of the connection portion 17 in a liquid-tight state. A pair of syringes can be used in place of the peristaltic pump device 55. The apparatus main body 21 is placed on the substrate part 12 of the case part 11, aligned with the connecting part 17, and aligned by pressing the pair of positioning pieces 16 placed on the substrate part 12 with the adjusting screw 15. Tighten and fix. The case portion 11 to which the apparatus main body 21 is fixed is fixed to the prescribed sample mounting portion B of the X-ray diffractometer by the mounting plate 19 with bolts 19b.

  In this state, the peristaltic pump device 55 causes the solution to flow in the holding hole 27a through the solution flow holes 29 and 36 at a predetermined ratio, so that the solution flows through the sample storage member 22 having solution permeability and the sample storage hole. The solution flows uniformly through the skin 1 which is a soft sample accommodated in 23. Therefore, the concentration of the chemical substance dissolved in the solution is kept constant in the sample accommodation hole 23, and the lipid extraction from the skin 1 is performed under a constant condition. As described above, the skin 1 is fixed so as not to move into the sample accommodation hole 23 by the holding metal fitting 25, the covering material 38, and the pair of case metal fittings 41 and 42, and the solution is allowed to flow into the sample accommodation hole 23. By irradiating the skin 1 with X-rays through the beam passage hole 47 by the line diffractometer, measurement can be performed under stable measurement conditions. As a result, in this example, even when a minute change such as the structural change of the intercellular lipid of the skin 1 is observed while flowing a solution such as glycerol, the difference in the observed structure is due to the contribution of glycerol. It is possible to distinguish whether it is due to individual differences of the skin 1 or not.

Next, specific examples 1 and 2 of the above embodiment will be described. In X-ray diffraction, the wavelength of X-ray was 0.1 nm, the camera length was 400 mm, and the scattering vector S = (2 / λ) sin θ was observed in the range of ⁰ to 3 nm ⁻¹ . However, 2θ is a scattering angle. The sample uses a hairless mouse stratum corneum, the sample is confined in the apparatus main body 21, the sample is filled with the solution, and the solution is injected. Then, the time change of the X-ray diffraction image is changed to 300 seconds over 2 to 3 hours. Observation was performed at an exposure time of 30 seconds every time. Within the observed S range, a diffraction image by the lamellar structure of the intercellular lipid in each layer and a diffraction image by the lattice constant of the hydrocarbon chain filling could be obtained simultaneously.

Specific Example 1 Fig. 4-1 and Fig. 4-2 are graphs showing the time changes of small-angle and wide-angle diffraction images when chloroform / methanol is applied to the skin. is there. In the figure, the passage of time changes from the upper curve to the lower curve. Furthermore, Figure 5-1, Figure 5-2, the wide-angle diffraction ^2.44Nm -1 when the action of chloroform-methanol to the skin, is a graph showing the time variation of the integrated intensity of 2.70 nm ^-1.

Figure 4-1 and FIG. 4-2, scattering vector S is small-angle diffraction image and the scattering vector S of 0～0.40Nm ^-1 are both wide-angle diffraction pattern of 2.0～3.0Nm ^-1, time It decreases with. The time variation of the integrated intensity of the lattice constant (reciprocal of the scattering vector S) of 0.41 nm of the wide-angle diffraction image changes as shown in FIG. Further, the change over time in the integrated intensity of the lattice constant of 0.37 nm in the wide-angle diffraction image changes as shown in FIG. 5-1 and 2 show that both decay with a relaxation time of about 2000 seconds. This gives time to extract lipids from the skin. As can be seen from the diffraction image in FIG. 4-2, the 0.41 nm and 0.37 nm diffraction peak widths hardly change, so that the 0.41 nm domain and the 0.37 nm domain have a structure within the domain over time. It can be considered that the domain region has shrunk without significantly changing. The packing structure of the hydrocarbon chain includes hexagonal crystal and orthorhombic crystal, the lattice constant of hexagonal crystal is 0.41 nm, and the lattice constant of orthorhombic crystal is 0.41 nm and 0.37 nm. At 41 nm, the diffraction peaks of the two structures are superimposed, and the two cannot be distinguished. Since the small-angle diffraction intensity in the range of 0.12 to 0.24 nm ⁻¹ in FIG. 4-1 is almost uniformly attenuated, lipids are extracted almost in the same manner in the packing structure of both hydrocarbon chains. Can be considered.

Specific Example 2 The amount of water in each layer and the effect of the glycerol solution FIGS. 6-1, 6-2 and 6-3 show the time change and the small angle diffraction of the small angle diffraction image when the glycerol solution is applied to the horny layer having the water content of 10% by weight. It is a graph which shows the time of the difference of an image, and the time change of wide angle diffraction (2.44nm < ^-1 >). FIGS. 7-1, 7-2, and 7-3 show the time change of the small-angle diffraction image, the time difference of the small-angle diffraction image, and the wide-angle diffraction when the glycerol solution is applied to the stratum corneum having a water content of 30% by weight. It is a graph which shows the time change of (2.44nm < ^-1 >). In FIGS. 6A and 6B, the passage of time changes from the lower curve to the upper curve, and in FIGS. 7A and 7B, the passage of time changes from the upper curve to the lower curve.

  A glycerol solution having a concentration of 10% was allowed to act on the stratum corneum having a water content of 10% by weight and 30% by weight, and the structural change of the intercellular lipid was observed. It is known that when the water content in the stratum corneum is about 20% by weight, the structure is almost natural, and the structure formed by intercellular lipids becomes unstable even if the water content is smaller or larger. Here, the effect of glycerol was examined by observing the structural change when glycerol, which is said to have a moisturizing action, was applied to the stratum corneum of the moisture content before and after that. The change in the diffraction pattern in the small-angle region when the water content is 10% by weight after adding the glycerol solution is not large as shown in FIG. 6-1, but from the change in the difference from the initial diffraction image in FIG. It can be seen that the intensity of the small angle diffraction image is increased. On the other hand, the change in the diffraction pattern in the small-angle region when the water content is 30% by weight after the glycerol solution is added is not large as shown in FIG. 7-1, but the difference from the initial diffraction image in FIG. From the change, it can be seen that the intensity of the small-angle diffraction image is reduced or almost unchanged. The wide-angle diffraction image was the same change.

  Furthermore, with respect to the change over time of the integrated intensity of the wide-angle diffraction image with a lattice constant of 0.41 nm, the integrated intensity increases with time when the water content is 10% by weight as shown in FIG. As shown in -3, when the water content is 30% by weight, the integrated intensity decreases with time or hardly changes. For this reason, when glycerol was allowed to act on the stratum corneum with a small amount of water, the structure of the intercellular lipid was stabilized, and the state in the case where the water content was 20% by weight was tried to approach. In some cases, the structure of intercellular lipids does not change so much, and it is considered that the state in the case of 20% by weight of water is maintained. Therefore, from these structural change experiments, it was found that glycerol plays an important role in water retention in the stratum corneum at the molecular level.

  In the above specific examples 1 and 2, typical measurement examples have been shown. By using the present invention, the X-ray diffraction experiment of the stratum corneum with various solutions is carried out with high sensitivity for cosmetic materials and transdermal absorption. It is possible to examine the effect of the accelerator at the molecular level. In addition, in the said Example, although the structure of skin was analyzed with the X-ray-diffraction apparatus, it is not restricted to this, Fiber structures, such as cellulose, and film structures, such as skin, using beam measuring devices, such as infrared rays absorption The present invention can also be applied when analyzing the structure of a soft material such as a three-dimensional structure of food. In addition, what was shown in the said Example is an example, It is also possible to implement in various changes in the range which does not deviate from the meaning of this invention.

  In the present invention, a soft material sample is inserted into a sample accommodation hole of a plate-shaped sample accommodation member having solution permeability, and fixed in a sample accommodation hole by a holding metal fitting, a covering material, and a pair of case metal fittings. Even when observing minute structural changes while flowing the solution through the sample in the state, it is possible to distinguish whether the observed structural difference is due to the contribution of the solution or due to individual differences. is there.

It is a top view which shows the soft material holding | maintenance apparatus used for the structural analysis of the skin which is one Example of this invention. FIG. 2 is a partially cutaway front view in the II-II line direction of FIG. 1 showing a soft material holding device. It is a partially broken front view which expands and shows the apparatus main body of a soft material holding | maintenance apparatus. It is a graph which shows the time change of a small angle diffraction image when chloroform methanol is acted on skin. It is a graph which shows the time change of the wide-angle diffraction image when chloroform methanol acts on skin. It is a graph which shows the time change of the integrated intensity | strength of the wide angle diffraction 2.44nm- ¹ when chloroform methanol acts on skin. It is a graph which shows the time change of the integrated intensity | strength of wide-angle diffraction 2.70nm- ¹ when chloroform methanol acts on skin. It is a graph which shows the time change of a small angle diffraction image when a glycerol solution is made to act on a stratum corneum with a moisture amount of 10 weight%. It is a graph which shows the time change of the difference of a small angle diffraction image when a glycerol solution acts on the stratum corneum of 10 weight% of water | moisture contents. It is a graph which shows the time change of wide angle diffraction (2.44nm < ^-1 >) when a glycerol solution acts on the stratum corneum of 10 weight% of moisture. It is a graph which shows the time change of a small angle diffraction image when a glycerol solution is made to act on a stratum corneum with a moisture amount of 30 weight%. It is a graph which shows the time change of the difference of a small angle diffraction image when a glycerol solution is made to act on a stratum corneum with a moisture amount of 30 weight%. It is a graph which shows the time change of wide angle diffraction (2.44 nm < ^-1 >) when a glycerol solution is made to act on a stratum corneum with a moisture amount of 30 weight%.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 ... Soft material holding device, 11 ... Case part, 12 ... Board | substrate part, 17 ... Connection part, 21 ... Apparatus main body, 22 ... Sample accommodation member, 23 ... Sample accommodation hole, 25 ... Holding metal fitting, 26 ... Central metal fitting part, 27a ... Holding hole, 29 ... Solution flow hole, 31 ... Outer ring metal fitting part, 32, 33 ... Upper and lower part, 36 ... Solution flow hole, 38 ... Cover material, 41, 42 ... Case metal fitting, 43, 44 ... Central convex part , 47, 48 ... beam passage holes, 55 ... perista pump device, 56 ... solution supply / discharge pipe.

Claims (5)

A soft material sample is inserted into the sample-containing sample-receiving member of the plate-like sample-containing member provided with the sample-containing hole, and has a holding hole, and a solution circulation hole connected to the holding hole at two locations on the outer periphery The sample holding member is inserted into the holding hole of the plate-shaped holding metal fitting having a thin coating material on both surfaces of the holding metal fitting to cover the sample holding hole in a liquid-tight state, and the sample holding hole is formed. A pair of case fittings provided with a beam passage hole arranged so as to be enclosed are sandwiched from both sides of the holding fixture via the covering material and are fixed to the holding fixture by fastening means. A structure analysis method for a soft material by beam irradiation, wherein a solution is circulated through the sample receiving hole through the beam and measurement is performed by irradiating the sample with a measurement beam through the beam passage hole by a beam measuring device. The structure analysis method for a soft material by beam irradiation according to claim 1, wherein a filter paper is used as the sample storage member. The structural analysis method for a soft material by beam irradiation according to claim 1 or 2, wherein a difference between measurement results obtained by the beam measurement device in a state where the solution is not introduced into the sample accommodation hole and in a state where the solution is introduced is obtained. . A plate-shaped sample accommodating member having a solution permeability provided with a sample accommodating hole into which a sample of a soft material is inserted, a holding hole for accommodating the sample accommodating member, and the holding hole at two locations on the outer periphery. A plate-shaped holding metal fitting having a solution flow hole to be connected, a pair of thin coating materials arranged in close contact with both sides of the holding metal fitting to cover the sample receiving hole in a liquid-tight state, and the holding metal fitting on both sides A pair of case fittings provided with a beam passage hole arranged on the side through the covering material and surrounding the sample receiving hole, and clamped and fixed to the holding fixture by clamping means. And a soft material holding device used for structural analysis of the soft material by beam irradiation, wherein a solution flow means for flowing the solution into the sample accommodation hole through the solution flow hole is provided. The soft material holding device used for the structural analysis of the soft material by beam irradiation according to claim 4, wherein a filter paper is used as the sample storage member.

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