JP2008017815A - Anti-menopausal syndrome food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-menopausal syndrome food capable of relieving/ameliorating menopausal syndrome. <P>SOLUTION: This anti-menopausal syndrome food comprises royal jelly and placental extracts as the active ingredients. The food can relieve/ameliorate complain of general malaise in menopause by autonomic nerve regulatory effect by the ingredients. Especially, each royal jelly and placental extracts shows no oestrogen-like action, so there is no fear of causing a problem of hormone balance disorder even if excessively ingesting. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to anti-menopausal foods. More specifically, the present invention relates to an anti-menopausal food that can relieve and improve indefinite complaints during menopause, using royal jelly and placenta extract as active ingredients.

Now that women's social advancement has become commonplace, societies have become more sophisticated, and nuclear families have become more advanced, women are faced with a greater variety of stresses and more problems than ever before. Among them, the biggest problem for women who have entered menopause is menopause. Climacteric disorder is an indefinite complaint syndrome caused by autonomic and psychogenic manifestations in menopause, causing unpleasant symptoms and affecting daily life. Symptoms of this indefinite complaint include, for example, general malaise, fatigue, slight fever, head weight, headache, hot flashes, tinnitus, numbness, palpitation, and cold limbs. Treatment for indefinite complaints includes hormone replacement therapy such as estrogen, but it is contraindicated for people with high blood pressure, diabetes, uterine fibroids and other diseases, and breast cancer and endometrial cancer are more likely to occur. Can not be taken. Therefore, it has been desired to develop a supplement that can cope with these indefinite complaints without side effects.
Conventionally, foods containing estrogen-like substances such as soybean isoflavones, black cohosh, and red clover have been ingested as supplements suitable for such menopause (see Patent Documents 1 to 3). However, in the case of supplements containing such estrogen-like substances, there has been a problem of losing hormone balance due to excessive intake due to the difference in sensitivity to estrogenic effects. Therefore, the Food Safety Committee has reported that the intake of soy isoflavones by supplements is set to an upper limit of 30 mg (aglycone equivalent) per day.
JP 2003-2831 A Special table 2004-534058 gazette JP 2002-142717 A

In view of such a current situation, the present inventors have made an active ingredient a substance having no estrogen-like action and have eagerly studied foods that can alleviate / improve climacteric disorder, and have been used as a medicinal agent for immortality and longevity since ancient times. The present invention was completed by finding that a combination of royal jelly and placenta extract can be an effective and safe functional food (supplement) against indefinite complaints during menopause.
This invention was made | formed based on the knowledge which concerns, and this invention provides the anti-menopausal foodstuff which can aim at relaxation and improvement of a menopause disorder.

  The gist of the present invention made to solve the above-mentioned problems is an anti-menopausal food containing royal jelly and placenta extract. In particular, the food preferably contains royal jelly and placenta extract (powder) at a ratio of 1:11 to 11: 1 (weight ratio), and further preferably contains a red clover extract and / or a ginseng extract.

  The anti-menopausal food of the present invention contains royal jelly and placenta extract as active ingredients, and both such royal jelly and placenta extract are not estrogen-like substances. There is no fear. And by the regulatory action of the autonomic nerve by the said component, the indefinite complaint in the menopause can be eased and improved.

As described above, the anti-menopausal food of the present invention comprises royal jelly and placenta extract as active ingredients.
As already known, the royal jelly used in the present invention is a milky white substance secreted by bees to the royal kingdom, and contains various useful ingredients such as proteins, carbohydrates, lipids, etc., and serves as a food for the queen bee. It is. Such royal jelly can be collected from the royal kingdom as described above, but a wide variety of products are already on the market, and such commercially available products can also be used.

  Royal jelly can be divided into a protein fraction and a protein-free fraction, and products that have been deproteinized are usually sold. The inventors of the present invention tested the synergistic effect with placenta extract using royal jelly that had been deproteinized and those that had not been deproteinized. To improve indefinite complaints during menopause, A strong synergistic effect with placenta extract could be recognized by using non-royal jelly. From this, in the present invention, the royal jelly indicates a raw royal jelly that is a royal jelly containing a protein component derived from a royal jelly that has not been subjected to deproteinization treatment.

  In addition, by replacing 2-20% of the protein fraction of royal jelly with the enzyme degradation product of the protein fraction of royal jelly, the time of manifestation of the effect of improving indefinite complaints is manifested when the enzyme degradation product is not used It was confirmed that it was accelerated from 20% to 40% of the time depending on the substitution amount. This effect is considered to be due to an increase in absorbability of the active peptide group derived from the above-mentioned royal jelly protein fraction.

The protein fraction of royal jelly can be prepared according to a conventional method. For example, after adding a hydrous hydrophilic solvent (for example, 70% ethanol) to a freeze-dried product of fresh royal jelly and stirring, the operation of filtering is repeated several times, and the extraction residue (cake) is squeezed and then freeze-dried, etc. The crude royal jelly protein fraction can be obtained by drying by an appropriate method. The crude royal jelly protein fraction can be obtained by purifying the crude royal jelly protein fraction by a conventional method such as dialysis or column purification.
The dehydrated royal jelly can be obtained by collecting, concentrating and purifying the hydrous hydrophilic solvent subjected to the above extraction.

Enzymatic degradation products of royal jelly protein substances can also be prepared according to conventional methods. For example, the above crude or purified royal jelly protein fraction is enzymatically degraded with a protease. The enzyme used in this case is not particularly limited as long as it is a protease capable of degrading royal jelly protein, and examples thereof include bromelain, papain, trypsin, and pepsin.
More specifically, the crude or purified royal jelly protein fraction is dissolved in a buffer solution or purified water adjusted to the optimum pH of the protease to be used, and then a predetermined amount of protease is added, and it is suitable at the optimum temperature of the protease. Incubate for a long time (for example, about 24 hours) to hydrolyze the royal jelly protein fraction. After digestion, heat (for example, at 100 ° C. for about 5 minutes) to inactivate the enzyme, allow to cool, neutralize to pH 7.0, centrifuge, freeze-dry the supernatant, royal jelly protein fraction enzyme digest (Peptide) can be obtained.
The royal jelly protein fraction enzyme degradation product (peptide) thus obtained can be purified by conventional methods such as gel filtration, ion exchange column chromatography, and reverse phase high performance liquid chromatography.

In addition, the placenta extract, which is another component used in the present invention, is a placenta (placenta) of a mammal (eg, human, pig, cow, sheep, etc.) treated with an enzyme, acid or alkali, or hot water. It can be obtained by purifying the components obtained by decomposition. There is a product already marketed as a placenta extract, and it may be used.
An example of a method for producing placenta extract is a method of treating pig placenta with a protease. Examples of the protease include papain, trypsin, pepsin, promeline, thermolysin and the like. Of these, papain is preferably used from the viewpoint of protease treatment efficiency. The protease treatment is advanced by reacting at 30 to 75 ° C. for about 0.5 to 3 hours, and the components obtained by the protease treatment are subjected to ultrafiltration, diatomaceous earth filtration, ion exchange resin, reverse osmosis filtration, activated carbon treatment. A placenta extract can be obtained by purifying by, for example.
The placenta extract can be pulverized by conventional means such as freeze-drying. In the present specification, the placenta extract (powder) means a powdery placenta having a water content of about 10% or less.

  In the present invention, royal jelly and placenta extract are used in combination. The blending amount of the royal jelly and the placenta extract is not particularly limited as long as the effect of improving the indefinite complaint is obtained, but preferably the royal jelly: placenta extract (powder) = 1: 11 to 11: 1 (weight ratio) ), More preferably 1: 4 to 4: 1 (weight ratio), and still more preferably 1: 2 to 2: 1 (weight ratio).

  The intake amount of the anti-menopausal food of the present invention can be appropriately adjusted according to the degree of symptoms of indefinite complaints, body weight, age, etc., but the total amount of royal jelly and placenta extract (powder) is usually 300 mg to 7000 mg. / Day, preferably 1000 mg to 6000 mg / day, more preferably 1500 to 5000 mg / day, and the dose may be taken once or divided into several times a day.

You may mix | blend a red clover extract further with the anti-menopausal foodstuff of this invention. The red clover extract contains an estrogen-like substance, and is effective in improving indefinite complaints during menopause. Red clover extract is a supplement that is worried about harmful effects caused by overdose, but in the presence of placenta extract and royal jelly, a significant increase in effect is recognized due to a synergistic effect. By adding the red clover extract so as to be 10 to 30 mg / day, which is an intake amount that does not become excessive, it is possible to avoid the problem of hormonal balance disturbance.
Furthermore, it was confirmed that the desired synergistic effect can be obtained by adding ginseng extract (ingestion amount of 10 to 150 mg / day) having various effects such as blood circulation promotion.

  Furthermore, ingredients commonly used in this field can be added to the anti-menopausal food of the present invention, if necessary. For example, jujube extract and narco lily extract 10 to 150 mg / day, shiitake mycelium extract and plum meat extract 5 to 50 mg / day, bear moth extract and pine needle extract 5 to 40 mg / day, ginkgo biloba Leaf extract, perilla leaf extract and cinnamon extract 5-20 mg / day, vitamin B1 0.3-25 mg / day, vitamin C 24-24 mg / day, vitamin E 2.4-150 mg / day, vitamin B2 0.33-12 mg / day Examples of vitamin B6 are 0.3 to 10 mg / day.

  According to the anti-menopausal food of the present invention, indefinite complaints during menopause can be alleviated / improved, but another advantage is that skin aging, which is a major problem for women of the same age, can be prevented at the same time. Placenta extract and royal jelly are known to have an effect on the skin alone, but a salivary gland hormone-like substance called “parrotin”, a substance unique to royal jelly, promotes skin metabolism, and the collagen in placenta extract Peptides and hyaluronic acid work by different mechanisms that suppress skin aging, and it is considered that the effect of preventing skin aging has been dramatically increased by the combined use. In particular, one of the indefinite complaints is depressive symptoms, but this effect is considered important for women because there is a risk that depressive symptoms will be further advanced by experiencing serious skin aging every day.

The anti-menopausal food of the present invention is ingested by humans as it is in various forms such as a solid or food and drink.
As a solid, the above-mentioned components are mixed with necessary components in the formulation such as appropriate physiologically acceptable additives (for example, carriers, excipients, diluents, etc.) as necessary, It can be obtained by preparing it in an appropriate dosage form, and examples of the form include tablets, powders, granules, capsules and the like.

Examples of food and drink include beverages (eg, drinks, milk beverages, coffee beverages, tea beverages, green tea beverages, juices, etc.), confectionery (eg, biscuits, cookies, candy, snack confectionery, ramune confectionery, etc.) , Added to seasoning liquids (eg, tart juice), meat products (eg, ham, sausage, etc.), fish products (eg, kamaboko, chikuwa), dairy products (eg, butter, cheese, etc.) Ingest.
Such food and drink can be prepared according to a conventional method except that an active ingredient is added in an appropriate process in the preparation stage. In addition, conventional additives may be added to such foods and drinks as necessary. Examples of such additives include vitamins (for example, vitamin C, vitamin A, vitamin E, etc.), minerals (For example, zinc, copper, manganese, etc.), physiologically active substances, sweeteners, acidulants, antioxidants, fragrances, salts, excipients, colorants and the like.

  Hereinafter, although this invention is demonstrated in detail based on a test example and a manufacture example, this invention is not limited to the example which concerns.

In the following examples, the materials and test methods used are as follows.
(1) Royal jelly: Commercially available raw jelly was used.
(2) Placenta extract: A commercially available placenta extract (powder) derived from porcine placenta was used.
(3) Royal jelly protein fraction:
The primary extraction was performed by adding 14 liters of 70% ethanol to 3 kg of freeze-dried fresh royal jelly, and 5.5 liters of 70% ethanol was again added to the cake (extraction residue) obtained by filtration. This was again filtered and the resulting cake was squeezed. The cake thus obtained (2.5 kg, wet weight) was vacuum-dried at 45-50 ° C. for 16 hours to obtain a crude royal jelly protein fraction. The protein fraction was suspended in deionized water and gently stirred for 1 hour while adjusting the pH to near neutral with a sodium hydroxide solution. After stirring, the suspension was centrifuged, and the supernatant was freeze-dried to obtain a royal jelly protein fraction.
The 70% ethanol extract was collected and concentrated to obtain a royal jelly deproteinized substance.
(4) Royal jelly protein fraction enzyme degradation product (peptide):
The above royal jelly protein fraction was dissolved in 0.1 M sodium phosphate buffer (pH 7.8), trypsin was added, and hydrolysis was performed at 37 ° C. for 24 hours. After decomposition, the enzyme was inactivated by heating at 100 ° C. for 5 minutes, and then allowed to cool and then neutralized to pH 7.0. After centrifugation (18,500 × g, 4 ° C., 20 minutes), the supernatant was freeze-dried to obtain a royal jelly protein fraction enzymatic degradation product.

Method for Measuring Skin Water Retention Capacity Since the stratum corneum of the epidermis contains electrolytes such as salts and amino acids, it becomes electrically conductive when water is present. Since there is a correlation between the electric conductivity and the water content, the moisture in the stratum corneum can be measured by measuring the electric conductivity. Therefore, a biological stratum corneum water load test was performed to measure the moisture retention ability of the skin. Wash the inner part of the volunteer woman from the elbow to the hand with soap, and after washing well, gently wipe excess water with a paper towel and let it rest in a room with constant temperature and humidity for 30 minutes. The conductivity (μS: micro-Siemens) (SKICON-2000 manufactured by IBS) was measured. The value obtained by measuring skin moisture before this test was about 40 μS on average. Compared to this value, the state of 1.5 times was taken as 5, and the interval between the two was divided into approximately 5 equal parts as follows to evaluate the degree of increase in the water retention capacity of the skin (1: 40-44, 2: 45-48, 3: 49-52, 4: 53-56, 5: 57-60).

Improvement of indefinite complaints , cheerfulness, and wrinkle improvement indefinite complaints were investigated by subject self-report using the simplified menopause index table (SMI) used as an objective evaluation method (see Table 1). . More specifically, vascular / motor symptoms are the highest (60 points) and lowest (0 points) of the total points (1) to (4), (9) and (10) in the table. The symptom (that is, the brightness of feelings) is evaluated by dividing the interval into five levels. The highest score (40 points) and the lowest score (0) of the total points (5) to (8) in the table are applicable. The evaluation was divided into five stages, and each was 1: no effect, 2: some effect, 3: some effect, 4: some strong effect, and 5: strong effect. In addition, wrinkle improvement was similarly evaluated in five stages based on the test subject's declaration.

Test example 1
The anti-menopausal food of the present invention was ingested by women in their 40s and 50s (10 people in each group) suffering from indefinite complaints of climacteric disorder for 1 month with the ingredients and intake shown in Table 2. During the intake period, questionnaires on indefinite complaints were taken regularly, and the amount of skin moisture (skin moisture retention ability) as an index of skin dryness was investigated. The results of questionnaires were tabulated for improvement of indefinite complaints and wrinkle improvement.
The results are shown in Table 2. In the table, test groups 1 to 5 and 14 to 17 are comparative examples.

  As shown in Table 2, ingestion of the anti-menopausal food of the present invention improved indefinite complaints and increased skin moisture. The increase in skin moisture at this time was considered to be due to a synergistic effect more markedly than when royal jelly or placenta extract was ingested alone. A more detailed questionnaire survey showed that skin dryness and wrinkles were improved, and that mental symptoms were particularly improved among the menopause index, and the feelings became brighter. It has been found that the above-described effects can be further enhanced when a red clover extract and / or ginseng extract is further added. In addition, comparing the results of Test Group 6 with those of Test Group 14 and Test Group 16, the former was superior in the development of the anti-menopausal disorder effect. Therefore, the anti-menopausal disorder effect by the combined administration of royal jelly and placenta extract was observed. That the test royal jelly contains both the royal jelly-derived protein component and the royal jelly deproteinization component, that is, the raw royal jelly containing both the royal jelly-derived protein component and the non-protein component is more effective. confirmed.

Test example 2
In order to confirm the effect of the royal jelly protein fraction enzyme degradation product (peptide) on the improvement of indefinite complaints and the effect of the improvement of indefinite complaints on the timing of the occurrence of the same test, the same test as described above was performed. That is, the anti-menopausal food of the present invention was ingested by women in their 40s and 50s (10 people per group) suffering from indefinite complaints of menopause for 1 month with the ingredients and intake shown in Table 3. A questionnaire regarding indefinite complaints was taken regularly during the intake period.
The results are shown in Table 3.

  As shown in Table 3, by replacing 2 to 20% of the protein fraction of the royal jelly with the enzymatic degradation product of the royal jelly protein fraction, the strength of the effect of improving indefinite complaints was the same as that of the royal jelly. It was confirmed that the onset of the effect was accelerated by about 20 to 40% depending on the substitution amount.

Production Example 1
Manufacture of capsules After mixing and homogenizing 2.2 parts by weight of royal jelly, 0.2 parts by weight of placenta extract (powder) and 1 part by weight of lactose, they were filled into hard capsules according to a conventional method, and capsules having an internal volume of 250 mg An agent was produced.
Production Example 2
Citric acid (0.2 parts by weight), orange fruit juice (60 parts by weight) and sugar (10 parts by weight) are heated and dissolved in water (28.28 parts by weight) for producing fruit juice beverages , and further 1 part by weight of royal jelly, placenta extract (Powder) 0.5 part by weight and 0.02 part by weight of red clover extract were added, and after cooling, container filling and heat sterilization at 85 ° C. for 30 minutes were performed to produce a fruit juice drink.

Claims (3)

  Anti-menopausal food containing royal jelly and placenta extract.   The anti-menopausal food according to claim 1, comprising royal jelly and placenta extract (powder) in a ratio of 1:11 to 11: 1 (weight ratio).   The anti-menopausal food according to claim 1 or 2, comprising a red clover extract and / or a ginseng extract.