JP2008013512A - Method for producing deodorant sterilizer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for producing a deodorant sterilizer, by which the deodorant sterilizer comprising Bacillus natto exhibiting usability for an anti-fungal property and silver zeolite acting as an anti-bacterial agent and prepared to easily activate the Bacillus natto in an atmospheric moisture is provided. <P>SOLUTION: This method for producing the deodorant sterilizer comprises at least the Bacillus natto exhibiting usability for an anti-fungal property and silver zeolite for sterilizing prokaryotes, preferably further powder vinegar (citric acid), an auxiliary agent such as oligosaccharide or lactose, and edible talc (talc powder). The Bacillus natto used as the raw material includes edible Bacillus natto and Bacillus natto spores. When the Bacillus natto spores are used, the Bacillus natto spores are used in a range of 1.0×10<SP>6</SP>to 10<SP>9</SP>spores/g. The deodorant sterilizer is produced in one form selected from the group consisting of powder, powder spray, suspension, solid soap and aqueous solution. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a deodorizing and disinfecting agent, and more specifically, an edible natto bacterium, a polysaccharide effective for germination and growth thereof, and a chemical substance containing a naturally occurring product effective for suppressing prokaryotic microorganisms. A method for producing a deodorizing and disinfecting agent, which simultaneously achieves deodorization and sterilization by efficiently suppressing microorganisms that are detrimental and detrimental to the human body, such as molds around them, in combination. The present invention relates to a deodorizing and disinfecting agent produced by the method.

  Conventionally, powdered vinegar (citric acid) and food talc (talc powder) have been used for the purpose of deodorization. Powdered vinegar (citric acid) instantly exhibits a deodorizing effect, and its utility value is frequently used. Food talc (talc powder) is also used as a deodorant by adsorbing odors.

  In recent years, a deodorizing and disinfecting agent using a silver-based inorganic antibacterial material using silver such as silver zeolite has been used in various fields. This leads to rapid deodorization of adsorption by zeolite and sterilization of prokaryotes by charge transfer by silver ions (see, for example, Patent Document 1).

With the advancement of science and technology such as nanotechnology, substances that effectively suppress the growth of pronuclear microorganisms, such as silver zeolite, and increase the deodorizing effect have been put into practical use. These can, for example, suppress the growth of microorganisms that are harmful to the human body such as staphylococci, but on the other hand, they have been widely used for higher microorganisms such as molds, such as copper ions. In comparison, its growth inhibitory effect is inferior. In other words, when silver is used, it is effective against bacteria such as Escherichia coli, but it has the problem that it promotes its reproduction, rather than having a bactericidal effect against fungi such as fungi. .
JP 2003-137731 A

  On the other hand, there is Bacillus natto as a familiar fungus, and Bacillus natto has long been used as food. The edible natto bacillus is a bacillus that is harmless to the human body, producing a large amount of secretion that is effective for the degradation of organic substances such as proteolytic enzymes and polysaccharide degrading enzymes outside the body and the suppression of the growth of coexisting microorganisms. It belongs to the subtilis species. If a sufficient amount of Bacillus natto is used, the microorganisms that are suppressed around Bacillus natto are effective regardless of the type.

  Moreover, since Bacillus natto is sterilized very efficiently by contacting with free chloric acid groups derived from hypochlorous acid, for example, tap water, sterilization is easy.

  Therefore, the present invention has been made in view of the above, so that Bacillus natto, which exhibits its usefulness in mold prevention, is combined with an antibacterial substance, silver zeolite, so that the Bacillus natto is easily activated by the humidity of the atmosphere. It aims at providing the manufacturing method of the prepared deodorizing disinfectant, and the deodorizing disinfectant manufactured by the manufacturing method.

  As a result of diligent investigations to solve the above problems, we have developed a combination of natto bacteria that are useful for antifungal effects and antibacterial silver zeolite. The present invention has been completed by finding that the above problem can be solved by confirming the effective sterilization effect.

  That is, the above object is solved by the following means according to the present invention.

  The invention according to claim 1 is achieved by a method for producing a deodorant disinfectant characterized in that it contains at least natto bacteria and silver zeolite, and is prepared so that the natto bacteria can be easily activated by the humidity of the atmosphere. The

  According to the first aspect of the present invention, the deodorizing disinfectant prepared so that the natto bacteria can be easily activated by humidity of the atmosphere, including at least natto bacteria and silver zeolite. Effective sterilization including higher microorganisms such as fungi can be performed.

  The invention according to claim 2 is the invention according to claim 1, wherein the Bacillus natto is edible Bacillus natto or Bacillus natto.

  According to the invention described in claim 2, effective sterilization including higher microorganisms such as fungi such as fungi can be performed simultaneously with deodorization by using natto bacteria as edible natto or natto bacteria spores. it can.

The invention according to claim 3 is the invention according to claim 2, characterized in that the Bacillus natto spores are used in a range of 1.0 × 10 ^{6 to} 10 ⁹ / g.

According to the invention of claim 3, by using the Bacillus natto spore in the range of 1.0 × 10 ^{6 to} 10 ⁹ cells / g, deodorization and fungi such as fungi such as fungi are included. Effective sterilization.

  The invention according to claim 4 is achieved by a deodorizing and disinfecting agent produced by the production method according to any one of claims 1 to 3.

According to the invention described in claim 4, the deodorizing disinfectant comprising at least edible natto bacteria or preferably 1.0 × 10 ^{6 to} 10 ⁹ / g natto bacteria spores and silver zeolite is used to eliminate odor. At the same time, effective sterilization including higher microorganisms such as fungi such as mold can be performed.

  The invention according to claim 5 is the invention according to claim 4, wherein the deodorizing and disinfecting agent is any one shape selected from the group consisting of powder, powder spray, suspension, solid soap and aqueous solution. It is characterized by being.

  According to the invention described in claim 5, the deodorizing and disinfecting agent is deodorized by taking any one shape selected from the group consisting of powder, powder spray, suspension, bar soap and aqueous solution. At the same time, effective sterilization including higher microorganisms such as fungi such as mold is possible, and it is possible to provide a deodorizing and disinfecting agent in a form that is easy to operate and handle in various usage environments.

  According to the present invention, there is a deodorizing disinfectant prepared by combining natto bacteria that are useful for antifungal activity and silver zeolite that acts as a general antibacterial substance, and that is easily activated by the humidity of the atmosphere. By doing so, it is possible to provide an effective sterilization action including mold at the same time as deodorization.

  Hereinafter, the best embodiment of the present invention will be described.

In one embodiment of the present invention, a method for producing a deodorant disinfectant includes at least Bacillus natto that exhibits usefulness against mold and silver zeolite for prokaryotic sterilization, and further includes powdered vinegar (citric acid), oligo It is characterized by preparing supplements such as sugar or lactose and food talc (talc powder) and mixing them. An edible natto bacterium is used as the natto bacterium, and preferably a good deodorizing and sterilizing effect can be obtained by adjusting the natto bacterium spores to a range of 1.0 × 10 ^{6 to} 10 ⁹ cells / g. In addition, the edible Bacillus natto used by this invention is easily available in order to use commercially available Bacillus natto.

  Here, each composition is demonstrated.

  Silver zeolite sterilizes prokaryotic organisms by rapid deodorization by adsorption of the zeolite and charge transfer by the silver ions. In particular, silver zeolite is widely used as a silver-based inorganic antibacterial material. Zeolite is a kind of clay mineral called aluminosilicate mineral, which is safe for humans and the environment. Silver zeolite utilizes the ion exchange function of zeolite and stably holds silver in an ionic state. As shown in FIG. 1, the antibacterial feature of silver zeolite is its broad antibacterial spectrum, but in particular, the bactericidal effect of higher microorganisms such as fungi such as fungi is low.

  Therefore, the present invention provides a deodorizing disinfectant having an effect on fungi as well as bacteria by combining natto bacteria useful for sterilization of higher fungi such as fungi with silver zeolite. can do.

As described above, edible natto bacteria are used as natto bacteria, and preferably 1.0 × 10 ^{6 to} 10 ⁹ cells / g natto bacteria spores are used. When Bacillus natto exerts its effect, it normally takes several tens of minutes to activate. However, it is necessary to previously supplement with oligosaccharides or lactose that become nutrients of Bacillus natto as a feed in a weakly inactivated state. It is prepared so as to be easily activated by the humidity of the atmosphere in combination with agents, and can be sterilized by digestion of higher microorganisms such as fungi such as mold.

  Powdered vinegar (citric acid) has an immediate deodorizing effect and is a substance generally used as a component of a deodorant. Food talc (talc powder) is also a substance having a deodorizing effect, but in addition to the deodorizing effect by adsorption, it is used to protect Bacillus natto by moisturizing.

  Each of these components is prepared and mixed to obtain the deodorizing and disinfecting agent of the present invention. The deodorizing and disinfecting agent of the present invention is usually provided in a powder form, but also includes a product in which a device is added to a composition that achieves an inactivated state by dissolving or dissolving them in water. For example, it may be a powder spray, a suspension of an oily solvent, a solid soap, an aqueous solution of a liquid powder in a colloidal state, or the like.

  Specific examples carried out according to the present invention will be described in more detail below, but the present invention is not limited to these examples.

  As a typical example of the present invention, one method for producing the deodorizing and disinfecting agent of the present invention will be described. A flow chart outlining the method for producing the deodorant disinfectant of the present invention is shown in FIG.

Silver zeolite The powder which fixed the silver ion to the synthetic zeolite was used. Silver zeolite is a substance that has already been widely used as a disinfectant, and its preparation method is well known and will not be described in detail here. Moreover, the adsorption function can be fully utilized by using commercially available products (for example, product name Zeorum: manufacturer name Tosoh) and using particles of about 10 microns (200 mesh or more). In the manufacturing process, there is a step of heating to 200 ° C. or higher, which is sterilized. Silver zeolite is adjusted so that the content of the final product is 2.5% or more.

Bacillus natto Bacillus natto is cultured using edible Bacillus natto. Using commercially available natto bacteria, the bacteria are isolated, the bacterial species is identified by genetic engineering, and a strain that is an edible natto bacterium is used. The strain isolated by plate agar culture is inoculated and cultured in a flask filled with a rich nutrient medium supplemented with an amino source for culturing aerobic bacteria and glucose to obtain a seed strain for culture. The seed strain is scaled up in a jar culture up to about 50L, and after culturing, the bacterial species is confirmed, cooled, inoculated into a stub tube for room temperature storage, used as a room temperature storage strain, and a stabilizer such as sucrose Add several milliliters to a plastic heat-resistant container and store it as a frozen stock for production. Produced after thawing the cryopreserved seed strain, each plate agar was cultured at the same time as the storage strain removed from the stub tube stored at room temperature, and confirmed that the properties were the same with a microscope etc. Get a seed strain. After the seed strain for production is initially cultured at 20 to 50 L, the first stage culture solution is dispensed from several hundred milliliters to several liters in the second stage of production in accordance with the production scale. After culturing, endospore formation is initiated by rapidly eutrophication of medium conditions for endospore formation, lowering of the medium temperature, or changing to an environment in which growth is difficult. After the start, 12-24 hours after sufficient endospores are formed, a high concentration culture solution is obtained by a spray drying method or a filter method. A high concentration culture solution is autoclaved at 121 ° C. or higher and 1.2 atm or higher for 20 minutes or longer to kill the reproductive body. This treatment is called autoclave sterilization treatment. The high-concentration culture solution that has been autoclaved is freeze-dried to obtain a dry powder in the form of endospores (spores) that can survive for a long time. Next, the obtained dry powder is roughly pulverized by a rotary pulverizer or the like, and a classification of about 1 mm or less is obtained by a classifier. The obtained classification is finely pulverized to about 200 to 500 mesh using a collision type pulverizer or the like, and fine particles of about 10 microns or less are obtained using a cyclone type classifier or the like. The fine pulverizer can realize a fine pulverization operation by using, for example, a nano jet mizer manufactured by Aisin Nano Technology. The obtained fine particle bulk is appropriately diluted, suspended in water together with a surfactant to obtain a constant concentration of bacterial solution, and the number of bacteria is assayed. Finally, the fine particle powder is diluted with an oligosaccharide, which is a germination promoter for bacteria, using a V-type stirrer or the like to obtain a product of Bacillus natto spores of 1 × 10 ¹¹ or more.

The final product is sterilized and deodorized by using natto spore in the range of 1.0 × 10 ^{6 to} 10 ⁹ cells / g. If it is less than 1.0 × 10 ⁶ / g, it does not sufficiently deodorize, and if it exceeds 1.0 × 10 ⁹ / g, an odor peculiar to natto metabolites represented by natto may be generated.

  Moreover, the amount of natto bacteria is adjusted depending on the balance between the resolution and the odor generating ability and the application amount depending on the type.

  When live bacteria such as natto are mixed with the deodorant disinfectant, the effect of disinfection and deodorization by the decomposition action of the target object by live bacteria (C) and the addition of odors by odorous substances resulting from the metabolism of live bacteria ( S) interact with each other. C and S depend on nutrients (or decomposition targets) N present in the environment in which viable bacteria are introduced. Since C, S, and N are considered to be a mixture, they are considered as follows.

上式（１）、（２）のC、Sはともに菌の種類Bに依存して決まることから、

Since C and S in the above formulas (1) and (2) are determined depending on the type B of the fungus,

と表すことができる。
菌種が固定された場合、除菌消臭を目的として生菌を利用するためには、

It can be expressed as.
In order to use live bacteria for the purpose of sterilization and deodorization when the species is fixed,

上式の条件を満たすように菌の使用方法を設計する必要がある。

It is necessary to design the method of using the bacteria so as to satisfy the above formula.

  Equation (4) is a condition that can be achieved by appropriately adjusting the amount of bacteria according to the characteristics of the target N.

  The characteristics of N mentioned here are: (A) conditions under which bacteria B can sufficiently increase, (B) conditions under which bacteria B are difficult to increase but can be maintained as a reproductive body for a certain period, (C B) B is roughly divided into three conditions that cannot maintain the reproductive body.

  Here, (C) does not require bacteria B, that is, it is excluded because N is an extremely dilute condition and it is not necessary to disinfect and deodorize.

From the above, in order to select bacteria B, it can be concluded that it is practically advantageous if the ratio of the left side to the right side (= I _B ) after the elapse of time τ in equation (4) is sufficiently large.

For Bacillus natto, assuming 24 hours tau, generally, LogI _B was approximately 2-5.

  The experimental result about a certain kind of Bacillus natto is shown in FIG.

  From this result, it is optimal that the amount of bacteria in one application amount is 1 million or more and 100 million or less (per gram of application amount), preferably about 1 million (per gram of application amount), more preferably It is understood that if about 10 million bacteria (per gram of coating amount) are used, the decomposition activity can be efficiently used without worrying about the odor derived from the bacteria.

In addition, the amount obtained by multiplying the amount of bacteria per gram as described above by about 20 times is preferable as the amount of bacteria used when producing the deodorizing and disinfecting agent of the present invention. It is preferably used in the range of 0.0 × 10 ^{6 to} 10 ⁹ pieces / g.

As described above, the supplement is prepared by adjusting the sugar content necessary for germination of natto endospores according to the amount of Bacillus natto. The sugar to be used mainly comprises food additives such as oligosaccharides or lactose known for promoting the germination of microorganisms. In this example, an oligosaccharide was used as a supplement. As the oligosaccharide, a commercially available product is used, for example, isomalt-oligosaccharide (product name isomalt: manufacturer name Nikken Kasei). The adjuvant is also prepared using the crushing process as described above, and fine particles having a mesh size of about 200 mesh or more are used. By uniformly blending the number of molecules about 10 to 1,000 times the number of edible microorganisms to be mixed using a V-type stirrer or the like, germination of microorganisms is promoted.

Powdered vinegar (citric acid)
Citric acid can be obtained by using starch such as corn or sweet potato as a sugar content supplier and subjecting it to excessive fermentation with koji mold for alcohol fermentation. This is sterilized by filter sterilization, and a pure citric acid fraction is obtained by a liquid chromatography method, and water is evaporated to obtain crystal hydrate of powdered vinegar. This is crushed by the same method as described above and used as particles of about 10 microns. By adjusting to a concentration of about 0.5% under the same amount of moisture, it exerts a bactericidal action in a saturated humidity environment such as in a clothing climate and also has a buffered acidic / basic odor substance Neutralize.

Talc for food (talc powder)
After crushing commercially available talc powder in the same manner as described above, approximately 10 micron particles are prepared, and then autoclaving at 121 ° C and 1.2 atmospheres is performed for 20 minutes or longer, or at 400 ° C or higher. Food talc was prepared by heat sterilization for 1 hour or longer. As the talc powder, a commercially available product that is easily available and generally available was used.

  Moreover, in the deodorizing and disinfecting agent of the present invention, each component other than edible natto may be prepared by a known technique. The bulk powder obtained by the above treatment was used to obtain the deodorizing and disinfecting agent of the present invention.

  To 100 g of food talc, 3 g of silver zeolite, 10 million cfu / g of natto powder, 1.5 g of powdered vinegar (citric acid) and 1.2 g of isomaltoligosaccharide were added to obtain approximately 100 g of product. . Furthermore, if necessary, a quality maintaining agent such as a desiccant may be added as appropriate.

  In addition, as an additional micronization step, the mixture product or each component is preliminarily adjusted to a particle size of 1 micron or less by a crushing method by collision in the gas phase. And can be made transparent immediately. Even when the additional micronization step is not carried out, if the particle size is adjusted to about 10 microns, the powder color disappears in about several minutes to about 10 minutes due to moisture absorption and diffusion if the coating amount is sufficiently extended by about 500 mg. Therefore, even if the user uses it for daily goods such as clothes and shoes and the living environment, it can be used without a sense of incongruity.

  Referring to FIG. 4, the results of comparative studies in the above examples are shown depending on the presence or absence of silver zeolite and the presence or absence of Bacillus natto. In the figure, the solid lines in (A) and (B) indicate the presence or absence of silver (no natto bacteria), and the broken lines (C) and (D) indicate the presence or absence of natto bacteria (both silver is present). The test subject is a male in his 40s, and the measurement results of the armpit when applied to the skin (particularly the periphery of the armpit) and spent light work and office work at room temperature of 27 to 30 ° C. For 24 hours after application, the act of cleaning the body such as bathing was prohibited, and the body odor and odor intensity were measured using a commercially available scent sensor (scent sensor FPO2: manufactured by Futaba Electronics Co., Ltd.). As a result of the measurement, it was confirmed that both the haste and the slow effect were effective against a heavy fragrance (non-volatile at normal temperature and slightly volatile at body temperature). Moreover, although it remains a little with respect to a light (volatile) fragrance, since the odor intensity itself has decreased, it can be suppressed to such an extent that no odor can be perceived by human experience. As can be seen from FIG. 4, deodorization within 4 hours can be explained by the presence or absence of silver, but after 24 hours, only one example with natto bacteria showed a significant difference.

  As mentioned above, when live bacteria were blended, it was not classified into cosmetics and quasi-drugs, so it could not be applied to human skin, but combined with food-level safe ingredients, By using edible microorganisms such as Bacillus natto, oral safety, that is, it is guaranteed to be safe even in contact with the digestive tract leading to excretion from the mouth (feeding), thus ensuring the safety of the user Even if it is applied to the skin, it can be safely used.

  The greatest advantage of using live bacteria such as Bacillus natto used in the present invention is that odorous substance degradation and live bacteria themselves are decomposed by biodegradation over a long period of time by using a high survival rate of stable quality over time. A sterilizing effect can be obtained by decomposing bacteria such as molds adhering to the skin and clothes as nutrients for life support.

  Therefore, as is clear from the above examples, by combining the natto bacteria that exhibit usefulness in antifungal effect and the antibacterial substance of silver zeolite, it is effective including deodorizing and higher microorganisms such as fungi such as fungi. It is possible to provide a deodorizing and disinfecting agent that exhibits an excellent disinfecting effect. Furthermore, the deodorizing and disinfecting agent of the present invention can be in various forms, and not only in a normal powder form, but also in a composition that achieves an inactivated state by dissolving these in water or oil. For example, powder sprays, suspensions of oil-based solvents, solid soaps, aqueous solutions of liquid powders in a colloidal state, etc.

  The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to such specific embodiments, and various modifications and changes can be made within the scope of the gist of the present invention described in the claims. It can be changed.

It is a table | surface which shows the antibacterial effect of silver zeolite. It is a figure which shows the flowchart which outlined the manufacturing method of the deodorizing disinfectant | microbicide of this invention. It is a figure which shows the relationship between the decomposition | disassembly activity of the target object by Bacillus natto, and the resulting fragrance. In the Example of this invention, it is a graph which shows the result of having conducted comparative examination by the presence or absence of silver zeolite and the presence or absence of natto.

Claims (5)

  A method for producing a deodorizing and disinfecting agent, comprising at least natto bacteria and silver zeolite, wherein the natto bacteria are prepared so as to be easily activated by atmospheric humidity.   The method for producing a deodorizing and disinfecting agent according to claim 1, wherein the Bacillus natto is edible Bacillus natto or Bacillus natto. The method according to claim 2, wherein the Bacillus natto spore is used in a range of 1.0 x 10 ^{6 to} 10 ⁹ cells / g.   The deodorizing disinfectant manufactured by the manufacturing method as described in any one of Claims 1 thru | or 3.   The deodorizing and disinfecting agent according to claim 4, wherein the deodorizing and disinfecting agent is in any one shape selected from the group consisting of powder, powder spray, suspension, solid soap and aqueous solution. Agent.

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