JP2008011830A - Method for cultivating mushroom and method for reducing barrier to growth of mushroom - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for cultivating mushroom by which degerming in a mushroom-cultivating facility can be carried out even in a highly humid environment, and the high-quality mushroom can be grown by suppressing the disease thereby; and to provide a method for reducing a barrier to growth of the mushroom. <P>SOLUTION: The method for cultivating the mushroom including a cultivation method of growing an anlage after forming the anlage includes radiating a positive ion and a negative ion for a prescribed period in a cultivation process to degerm various germs in the facility. The method for reducing the barrier to the growth of the mushroom is characterized by the degerming of bacteria of the genus Pseudomonas by radiating the positive ion or the negative ion. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a method for cultivating mushrooms using positive and negative ions, and a method for reducing mushroom growth disorders.

  At present, mushrooms such as shiitake mushrooms, enokitake mushrooms and eringgi are representative ingredients that are indispensable for dining tables. In particular, from the producer's point of view, the prices of the three main products, shiitake, enokitake and beech shimeji, have declined significantly with the recent increase in mushroom cultivation and the increase in imported mushrooms. Expectations are growing. In general, however, mushrooms have poor resistance to harmful bacteria, and purification of the cultivation room is a major technical issue.

  For example, in mushroom cultivation, there are many examples of damage caused by growth disorders that occur from the first to the middle of growth, which is the biggest problem in mushroom cultivation. In particular, in eringi cultivation, there is an urgent need to suppress the “withering symptom”, which is one of the growth disorders. Here, the “withering symptom” refers to discoloration of the fruiting body in the process of growth. When the symptom is mild, coloring, bending, and dysplasia of the umbrella occur. A condition that causes atrophy or rot, and in severe cases leads to death. Alternatively, when the regenerated mycelium of the mycelium is discolored and no fruit body is formed, it can be regarded as a “withering symptom”.

  Here, as a purification method of a mushroom cultivation facility, there is a method of controlling harmful bacteria by spraying chemicals. However, in the purification by spraying the drug before growing the mushroom, even if the room can be completely cleaned, the entry of various germs cannot be denied due to the entry and exit of people during the mushroom growth. Also, the use of drugs during mushroom growth is not possible from the viewpoint of food hygiene and the Agricultural Chemicals Control Law. Furthermore, cleaning and purification of the mushroom cultivation room requires spraying urethane on the wall surface, etc., and it is currently difficult to perform structurally, and many facilities are difficult to clean and clean.

  For example, Patent Literature 1 discloses a method for controlling harmful bacteria using ozone gas as a method for controlling harmful bacteria other than spraying chemicals. However, although this method can sterilize harmful bacteria in mushroom cultivation facilities by the sterilization of ozone, ozone gas has an effect on the human body, so ozone gas is generated at night when no one is present, and the ozone gas concentration is reduced to 0.1 ppm or less. Until then, you cannot enter the mushroom cultivation facility. That is, it cannot enter into the mushroom cultivation facility during sterilization, and after the sterilization, it cannot enter the mushroom cultivation facility until the ozone concentration becomes 0.1 ppm or less, and the working time is limited. Moreover, even if the inside of the mushroom cultivation facility can be completely sterilized, miscellaneous germs may enter when a person enters and leaves the mushroom cultivation facility after sterilization.

  Patent Document 2 describes that a sterilization apparatus using a photocatalytic reaction is installed in a horticultural facility together with an air stirrer as a means for removing airborne bacteria in order to control diseases of horticultural horticultural crops. Has been. However, in order to sufficiently bring the air into contact with the photocatalyst, it is necessary to arrange the photocatalyst evenly around the crop, which limits the degree of freedom of farm work, reduces the crop cultivation area, and reduces the yield. There was a problem that. Moreover, when the scale of a horticultural facility increases, the equipment cost increases. Furthermore, since the light source for photocatalyst excitation must be constantly turned on, there is a problem that the running cost is increased.

Further, Patent Document 3 is characterized in that floating microorganisms existing in the facility are inactivated by releasing positive and negative ions from the ion generating device in the cultivation of fungi, breeding of livestock, or fishery products. A sterilization method for production facilities is described. However, Patent Document 3 does not describe a specific means for releasing positive and negative ions and achieving sterilization in a high humidity environment that is essential in some steps of mushroom cultivation, for example. Moreover, it does not describe the reduction of growth disorders such as withering symptoms, which is a problem in mushroom cultivation.
JP 2001-309724 A JP 2002-186364 A JP 2004-65614 A

  The present invention has been made in order to solve the above-mentioned problems, and its object is to eliminate sterilization in a mushroom cultivation facility even in a high humidity environment that is essential in several steps of mushroom cultivation. It is possible to provide a method for cultivating mushrooms that can suppress diseases and grow high-quality mushrooms.

  Another object of the present invention is to provide a method for reducing growth disorders that are problematic in mushroom cultivation.

  As a result of intensive studies, the present inventors released an ion and a negative ion into a mushroom cultivation facility using an ion generator that can generate a sufficient amount of positive ions and negative ions even in a high humidity environment. It was found that by sterilizing the germs in the facility, the disease was unexpectedly suppressed without affecting the cultivated mushroom itself and a high-quality mushroom was obtained.

  In addition, the present inventors have also sterilized using positive and negative ions, and are effective for eradicating Pseudomonas spp. Which is one of the causative fungi of mushrooms such as eringi, and release positive and negative ions. By this, it discovered that the growth disorder | damage | failure of a mushroom could be reduced effectively, without affecting the mushroom itself which is a cultivation object. That is, the present invention is as follows.

  The mushroom cultivation method of the present invention is a mushroom cultivation method including a cultivation process for growing a primordium after formation of a primordium. During this period, positive ions and negative ions are released to eliminate germs in the facility. Here, in the case where the cultivation process includes a sprouting process for forming a primordium and a growing process for growing the primordium, positive ions and negative ions are present in the facility where the sprouting process and / or the growing process is performed. Ions may be emitted. In addition, in the case where the cultivation process includes a sprouting process for forming a base, a leveling process for aligning the base, and a growth process for growing the base, in the facility where the leveling process is performed. In addition, positive ions and negative ions may be released.

Here, in the method for cultivating mushrooms of the present invention, it is preferable to release positive ions and negative ions so that the amount of positive ions and negative ions in the facility is 2000 or more / cm ³ , respectively.

  In the mushroom cultivation method of the present invention, the relative humidity in the facility may be in the range of 80 to 100%.

  Moreover, in the mushroom cultivation method of the present invention, the above-mentioned bacteria may be Pseudomonas sp. In this case, according to the method for cultivating mushrooms of the present invention, growth failure of mushrooms can be effectively reduced.

  Further, the present invention, after the formation of the primordial, in the facility where the cultivation process for growing the primordial is carried out, by releasing positive ions and negative ions for a certain period of time during the cultivation process, Disclosed is a method for reducing a growth disorder of a mushroom characterized by sterilizing Pseudomonas sp. Here, in the case where the cultivation process includes a sprouting process for forming a primordium and a growing process for growing the primordium, positive ions and negative ions are present in the facility where the sprouting process and / or the growing process is performed. Ions may be emitted. In addition, in the case where the cultivation process includes a sprouting process for forming a base, a leveling process for aligning the base, and a growth process for growing the base, in the facility where the leveling process is performed. In addition, positive ions and negative ions may be released.

Here, in the method for reducing the growth disorder of the mushroom of the present invention, the positive ions and the negative ions may be released so that the amount of positive ions and negative ions in the facility is 2000 or more / cm ³ , respectively. preferable.

  Moreover, in the method for reducing the growth disorder of the mushroom of the present invention, the relative humidity in the facility may be in the range of 80 to 100%.

  According to the mushroom cultivation method of the present invention, even if the inside of the mushroom cultivation facility is in a high humidity environment, it becomes possible to sterilize the mushroom cultivation facility by releasing positive and negative ions. Diseases are suppressed and high quality mushrooms can be obtained. Such release of positive and negative ions does not affect the mushrooms to be cultivated. In addition, since positive and negative ions are harmless to the human body, there is no limit on working time.

  Moreover, according to the method for reducing the growth disorder of the mushroom of the present invention, it is possible to effectively sterilize Pseudomonas spp. Existing in the mushroom cultivation facility under a high humidity environment, and the mushroom growth disorder is effective. Can be reduced.

  Furthermore, since the mushroom cultivation method and the method of reducing mushroom growth disorder according to the present invention can be carried out simply by installing an ion generator that generates positive and negative ions in an existing mushroom cultivation facility, a significant improvement of existing facilities, etc. Is not required.

<Mushroom cultivation method>
The mushroom cultivation method of the present invention is a mushroom cultivation method including a cultivation process for growing a primordium after forming a primordium, and in the facility where the cultivation process is carried out. It is characterized by releasing positive ions and negative ions for a certain period of time to sterilize germs in the facility.

  Thus, by releasing positive ions and negative ions to the facility where the cultivation process is carried out, it is possible to sterilize the germs in the facility. Thereby, disease can be suppressed and high-quality mushrooms can be obtained.

  Here, "miscellaneous bacteria" means bacteria other than the mushroom fungus to be cultivated. Further, “bacteria elimination” means that the bacteria are actively worked to suppress or stop the activities of the bacteria.

  Hereinafter, the cultivation method of the mushroom of this invention is demonstrated in detail, referring FIG. FIG. 1 is a flowchart schematically showing a preferred example of the mushroom cultivation method of the present invention. In addition, although the description about the cultivation method of the mushroom of this invention shown in FIG. 1 and the following is made | formed with eringi, it has the process substantially the same as FIG. 1 also about other mushrooms, and the mushroom of this invention It will be apparent that the cultivation method can be applied to mushrooms other than eringi as long as they belong to the field of the technology.

  In the mushroom cultivation method of the present invention, the medium is first prepared by a conventionally known method (step 101). Specifically, although not limited, for example, sawfish such as Sugiogako and nutrients such as bran, rice bran, and corn cob are mixed at a composition ratio that is usually employed in the field. Next, water is added and mixed so that the water content is about 60 to 70%. These can be mixed using a conventionally known method, for example, a mixer for stirring the medium.

  In the subsequent process, the culture medium is filled into a cultivation container made of polypropylene, for example, by a conventionally known method (step 102). The filling amount is not particularly limited, and an amount usually employed in the field can also be employed in the present invention. For example, when the capacity of the cultivation container is 850 ml, the amount of the medium to be filled can be set to about 530 g, for example.

  In the subsequent process, after the cultivation container is covered, the filled medium is sterilized by a conventionally known method (step 103). As the sterilization method, either high pressure sterilization or normal pressure sterilization may be used. In the case of high-pressure sterilization, the sterilization treatment is usually performed using a high-pressure sterilization pot. For example, after the medium temperature reaches about 120 ° C., the temperature is maintained for about 30 minutes, for example. The sterilized medium is aseptically cooled to 10 to 30 ° C., for example.

  In the subsequent step, the medium is inoculated with eringi seeds by a conventionally known method (step 104). The inoculation can be performed using, for example, an inoculator.

  In the subsequent step, the inoculum of the inoculated eringi is cultured by a conventionally known method, and subsequently ripened (step 105). By this process, the inside of the cultivation container spreads with mycelium. Usually, the culture is performed, for example, under conditions of about 21 to 23 ° C. and a relative humidity of about 60% for about 25 days, and ripening is performed by holding for about 5 to 10 days under the same conditions.

  In the subsequent process, the bacteria are scraped by a conventionally known method (step 106). Here, “bacteria scraping” refers to scraping the surface of a fungus bed in which mycelia have spread. Formation of a primordium can be promoted by such a fungus scraping treatment. As a method for scraping bacteria, for example, “bukikaki” can be mentioned. “Bukkaki” means a method of scraping the surface of the fungus together with the inoculum. In addition, when the bacteria are scraped, for example, a water injection process such as spraying may be performed.

  In the subsequent process, sprouting is performed (step 107). Here, “emergence” refers to the formation of a primordium. The sprouting is usually performed in a facility different from a culture room called a sprouting room and a culture room in which fungi are scraped. As environmental conditions in the germination room, conditions usually employed in mushroom cultivation can also be employed in the present invention. For example, the temperature in the germination chamber is about 15 to 20 ° C., and the relative humidity is about 80 to 100%, more preferably about 95 to 100%. Thus, a very high humidity environment is required in the normal sprouting chamber. In addition, sprouting is usually performed until the fruit body grows to about 2 to 3 cm. The period of the sprouting step is usually about 5 to 15 days.

  In the subsequent step, the formed bases are aligned and leveling is performed (step 108). As the environmental conditions in the facility where the leveling process is performed, conditions normally employed in mushroom cultivation can also be employed in the present invention. For example, the temperature in the facility can be about 16 ° C. and the relative humidity can be 95 to 100%. As described above, in a facility where the leveling process is normally performed (hereinafter referred to as a leveling room), a very high humidity environment is required. The period of the leveling process is usually about 3 days. Here, the leveling room may be a facility different from the germination room or the same facility as the germination room. Further, the leveling room may be a facility different from a facility (hereinafter referred to as a growth chamber) where a growth process (step 109) described later is performed, or may be the same facility. In addition, the said smoothing process is not an essential process in mushroom cultivation, and can also be abbreviate | omitted.

  In the subsequent process, the base is grown (step 109) and harvested (step 110). In this specification, the process from the germination step (step 107) for forming the primordium to the growth step (step 109) for growing the primordium is referred to as a cultivation step. Here, in this example, the cultivation process includes three processes from Steps 107 to 109, but additional processes other than this may be included in the cultivation process. Further, as described above, the leveling step (step 108) may be omitted.

  In the present invention, conditions usually employed in mushroom cultivation can be adopted as the environmental conditions in the growth chamber where the primordial growth is performed. For example, the temperature in the growth chamber is about 15 to 20 ° C., and the relative humidity is about 80 to 100%, more preferably about 90 to 95%. Thus, a very high humidity environment is required in the normal growth room. The period of the growth process is usually about 5 to 10 days. The germination room and the growth room may be different facilities or the same facility. Further, the leveling room and the growth room may be different facilities or the same facility.

  The mushroom cultivation method of the present invention is characterized in that positive ions and negative ions are released into a facility where the cultivation process is carried out to eliminate germs in the facility. By releasing such positive ions and negative ions, it is possible to sterilize the germs in the facility without adversely affecting the mushrooms themselves that are to be cultivated, the disease is suppressed, and high-quality mushrooms are cultivated Can do.

  Positive ions and negative ions released into the facility where the cultivation process is carried out act on various germs floating in the facility and suppress or stop the activity. Examples of the germs floating in the facility include Pseudomonas sp., Cladbotrium sp., Penicillium sp., Which are one of the causes of growth disorders.

  The facility where positive ions and negative ions are released is a facility where a cultivation process is performed, that is, one or more of a sprouting room, a conditioned room, and a growing room. By releasing positive ions and negative ions into at least one of the germination room, the conditioned room, and the growth room, sufficient disease control effect and high quality mushrooms are recognized. Positive ions and negative ions may be released also in the aging process (step 105) and the fungus scraping process (step 106). As described above, some or all of the germination room, the run-in room, and the growth room may be the same facility.

  The period during which positive ions and negative ions are released is a certain period during the cultivation process, that is, any one or more of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109). It is a certain period during the process. Even if the release of positive and negative ions limited to a part of the period is not the entire period during the cultivation process, a sufficient disease suppressing effect and high quality mushrooms are recognized.

  Specifically, the release of positive and negative ions may be performed only in any one of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109). For example, positive and negative ions may be released only in the leveling step (step 108). Alternatively, positive and negative ions may be released in two or more steps. For example, positive and negative ions may be released in the germination process (step 107) and the growth process (step 109). The same applies when the leveling step (step 108) is omitted.

  The timing for starting and stopping the release of positive and negative ions is not particularly limited. For example, when positive and negative ions are released only in the sprouting process (step 107), the timing of starting the release of positive and negative ions is transferred to the sprouting chamber after the cultivation container that has finished the fungus scraping process (step 106) is started. It may be before, about the same time, or after starting the sprouting process. In addition, the timing of stopping the release of positive and negative ions may be before moving to the facility for the next process (when the next process is performed in the same facility, at the end of the germination process), and at approximately the same time. Or later. In the case where positive and negative ions are released only in any one of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109), positive and negative ions are released over the longest possible period in the process. Is preferable, and it is particularly preferable that the entire period of the process be performed. If the release of positive and negative ions is extremely short, the quality of the mushrooms may be impaired. The same applies when positive and negative ions are released only in the leveling process (step 108) or the growth process (step 109).

  In addition, when the release of positive and negative ions is performed in any two or more of the sprouting process (step 107), the smoothing process (step 108), and the growth process (step 109), the positive and negative ions are released. The timing to stop is not particularly limited. For example, when the germination process (step 107) and the growth process (step 109) are performed in the same facility and positive and negative ions are released from the germination process (step 107) to the growth process (step 109), release of positive and negative ions is started. The timing to perform may be before the cultivation container that has finished the fungus scraping process (step 106) is moved to the budding chamber and the budding process is started, or may be approximately the same, after the budding process is started. There may be. The timing of stopping the release of positive and negative ions may be the same as the end of the growth process or may be after the end. Moreover, you may stop discharge | release of positive / negative ion before completion | finish of a growth process. Even when positive and negative ions are released in any two or more of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109), the positive and negative ions are extremely released. If the length is too short, the quality of the mushroom may be impaired. Therefore, it is preferable that positive and negative ions are released over as long a period as possible.

  In addition, the release of positive and negative ions can be performed during the cultivation process regardless of whether or not the two or more processes of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109) are performed. It may be performed continuously for a certain period or may be performed intermittently.

  As described above, the method for cultivating mushrooms of the present invention is characterized in that mushrooms are cultivated in a sterilization environment with positive and negative ions for a certain period during the cultivation process. Thus, the effect of improving the quality of the mushrooms is recognized even if the release of positive and negative ions only during a part of the period, not the entire period during the cultivation process.

(Method for generating positive and negative ions)
Here, a method of generating positive ions and negative ions will be described. For generating positive ions and negative ions, a conventionally known ion generator that generates both positive and negative ions by a discharge mechanism is used. The discharge mechanism here has, for example, a structure in which a ceramic dielectric is sandwiched between a discharge electrode and a dielectric electrode, and corona discharge is generated in the vicinity of the discharge electrode by applying a high voltage between the discharge electrode and the dielectric electrode. It is a mechanism that occurs and thereby generates positive and negative ions by ionizing or dissociating water molecules and oxygen molecules in the air. The positive ions and negative ions thus generated are mainly composed of H ⁺ (H ₂ O) _m (m is an arbitrary natural number) and O ₂ ⁻ (H ₂ O) _n (n is an arbitrary natural number), respectively. Ion. These H ⁺ (H ₂ O) _m and O ₂ ⁻ (H ₂ O) _n adhere to the surface of bacteria floating in the facility and cause a chemical reaction to generate H ₂ O ₂ and / or. OH is produced. Since H ₂ O ₂ and .OH show extremely strong activity, these actions can surround the bacteria and suppress or stop their activity. Here, .OH indicates radical OH.

  In the present invention, the voltage applied between the discharge electrode and the dielectric electrode, that is, the applied voltage is preferably a high voltage. As described above, the inside of the facility where the cultivation process for releasing positive and negative ions is performed is in a high humidity environment with a relative humidity of 80 to 100%, more preferably 90 to 100%. In such a high humidity environment, if the applied voltage is low, condensation occurs on the discharge portion of the electrode surface, and there is a possibility that discharge will not occur. Specifically, although it depends on the structure of the electrodes, a preferable applied voltage is at least 4 kV or more as a peak-to-peak voltage between the electrodes. In order to generate sufficient positive and negative ions even in a high humidity environment with a relative humidity of 90% or higher, it is particularly preferably about 4.5 kV or higher.

Here, Table 1 shows the relationship between the applied voltage that can be discharged and the relative humidity. FIG. 2 is a schematic device diagram used for measuring the dischargeable applied voltage shown in Table 1. As shown in FIG. 2, a 1 m ³ acrylic box 201 has a structure in which a ceramic dielectric is sandwiched between a discharge electrode and a dielectric electrode, and an AC voltage is applied between the discharge electrode and the dielectric electrode. An ion generator 202 that generates ions, and a blower 203 (DC8V) is installed at a position 90 mm away from the ion generation portion of the ion generator 202, and is on a straight line connecting the ion generator 202 and the blower 203, and on the leeward side In addition, an ion measuring device 204 (an ion counter manufactured by Andes Electric Co., Ltd.) was installed at a position 150 mm away from the ion generating portion of the ion generator 202. As shown in FIG. 2, the ion measuring device 204 is installed outside the acrylic box 201, but the acrylic box 201 is provided with a hole so that ions can be counted. Using such an apparatus, the applied voltage that can be discharged was measured at various relative humidity. The dischargeable voltage means a minimum voltage at which the number of negative ions counted by the ion measuring instrument 204 within 30 seconds after the voltage is applied exceeds 10,000 / cm ³ . The blower 203 was operated so that the wind speed in the vicinity of the ion generator of the ion generator 202 was 4 m / sec.

  As can be seen from Table 1, the ion generator having the above structure requires an applied voltage of at least about 3.5 kV when the relative humidity is 80% and at least about 4.5 kV when the relative humidity is 90%. I know that there is.

Further, in the same measuring apparatus as described above, an ion generator and a blower having the same structure were used, and the relationship between the positive / negative ion generation amount (pieces / cm ³ ) and the relative humidity when the applied voltage was different was examined. The results are shown in Table 2. The amount of positive and negative ions shown in Table 2 is an average value of both positive and negative ions at a distance of 10 cm from the outlet, and the blower is operated so that the wind speed in the vicinity of the ion generation part of the ion generator is 5 m / sec. It was.

As is apparent from Table 2, when the applied voltage is 3.5 kV as a peak-to-peak voltage between the electrodes, the amount of positive and negative ions decreases by about 40% at a relative humidity of 75%. It can be seen that positive and negative ions are not generated at 80% or more. Even in the background, that is, when the ion generator is not operated, there are about 200 to 500 ions / cm ³ ions. On the other hand, when the applied voltage is 5.3 kV as a peak-to-peak voltage between the electrodes, positive / negative ions of 100,000 ions / cm ³ or more are generated even if the relative humidity is 80% or more. I understand that.

  From these results, it can be seen that discharge does not occur in mushroom cultivation facilities that require a high humidity environment at an applied voltage of about 3.5 kV, which is commonly used for ion generators. Moreover, it turns out that it is necessary to make an applied voltage high in order to enable discharge in a high humidity environment such as a mushroom cultivation facility.

(Bacteria elimination in mushroom cultivation facility by positive and negative ion release)
Next, a method for releasing positive ions and negative ions into a facility where the cultivation process is carried out to eliminate germs in the facility will be specifically described.

  In the present invention, the ion generator as described above is installed and operated in a facility where the cultivation process is carried out to release positive ions and negative ions, thereby sterilizing germs in the facility. According to the method for cultivating mushrooms of the present invention, it is only necessary to install an ion generator, and it is not necessary to repair existing facilities. Further, as described above, the inside of the facility where the sprouting step (step 107) and the growing step (step 108) are performed is in a high humidity environment. Even under such conditions, it is possible to generate a sufficient amount of positive and negative ions to efficiently sterilize germs in the facility.

Here, the release of positive ions and negative ions in the facility where the cultivation process is performed is performed so that the amount of positive ions and negative ions in the facility is 2,000 / cm ³ or more, respectively. Is preferred. In particular, it is preferred that the amount of positive and negative ions is 2,000 / cm ³ or more, respectively, in the facility where the cultivated mushrooms are placed. When the number is less than 2,000 cells / cm ³ , there is a possibility that various germs in the facility cannot be sufficiently sterilized. In addition, since it is not easy to measure the amount of positive and negative ions at every location in a facility under a high-humidity environment, in the present invention, preferably, a simulation of ion distribution is performed using a computer to determine the amount of ions in the facility. Calculate (pieces / cm ³ ).

Calculation of ion quantity (number / cm ³ ) in the facility by simulation of ion distribution using a computer is performed by performing steady state analysis calculation using, for example, “STREAM” (CFD analysis software of Software Cradle Co., Ltd.). Can do.

One ion generator may be installed in one facility, or two or more ion generators may be installed. For example, when the space volume of the facility is large, the above-mentioned preferable ion environment, that is, an environment where the amount of positive and negative ions in the facility is not less than 2,000 / cm ³ is not achieved with one ion generator. It is preferable to install two or more ion generators. The installation location of the ion generator in the facility is not particularly limited. For example, it may be simply placed on the floor or attached to the side wall. Or you may install in a ceiling. Even when two or more ion generators are installed in one facility, the positional relationship between the ion generators is not particularly limited.

  Along with the installation of the ion generator, it is preferable to install a blower in the facility for sending the generated positive and negative ions to the facility space. The number of fans in one facility may be one or two or more. The blower is preferably installed near the ion generator. Thereby, the generated positive and negative ions are efficiently delivered to the facility space. In addition to the blower or instead of the blower, a fan for stirring the air in the facility may be installed on the ceiling or the like.

<Mushroom growth disorder reduction method>
The present invention relates to Pseudomonas by releasing positive ions and negative ions for a certain period of time during the cultivation process into a facility where the cultivation process for growing the primordium is performed after the formation of the primordium. Disclosed is a method for reducing a growth disorder of a mushroom characterized by sterilizing a genus fungus. Positive ions and negative ions can effectively suppress or stop the activity of Pseudomonas species, thereby significantly reducing mushroom growth disorders. In addition, the mushrooms to be grown are not adversely affected, and high-quality mushrooms can be obtained.

  The facility where positive ions and negative ions are released is a facility where a cultivation process is performed, that is, one or more of a sprouting room, a conditioned room, and a growing room. By releasing positive ions and negative ions into at least one of the germination room, the conditioned room, and the growth room, sufficient disease control effect and high quality mushrooms are recognized. Positive ions and negative ions may be released also in the aging process (step 105) and the fungus scraping process (step 106). As described above, some or all of the germination room, the run-in room, and the growth room may be the same facility.

  The period during which positive ions and negative ions are released is a certain period during the cultivation process, that is, any one or more of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109). It is a certain period during the process. Even if the release of positive and negative ions limited to a part of the period is not the entire period during the cultivation process, a sufficient disease suppressing effect and high quality mushrooms are recognized. The timing for starting and stopping the release of positive and negative ions is as described above.

  In addition, the release of positive and negative ions can be performed during the cultivation process regardless of whether or not the two or more processes of the sprouting process (step 107), the smoothing process (step 108), and the growing process (step 109) are performed. It may be performed continuously for a certain period or may be performed intermittently.

  Thus, the method for reducing the growth disorder of mushrooms according to the present invention is characterized by cultivating mushrooms in a sterilization environment with positive and negative ions for a certain period during the cultivation process. Thus, even if it is the release of positive and negative ions only in a part of the period, not in the entire period during the cultivation process, it is possible to effectively reduce growth disorders such as withering symptoms.

  The method for generating positive and negative ions in the mushroom growth disorder reducing method of the present invention is as described above. That is, a conventionally known ion generator that generates both positive and negative ions by a discharge mechanism is used to generate positive ions and negative ions. The inside of the facility where the cultivation process for releasing positive and negative ions is carried out is in a high humidity environment with a relative humidity of 80 to 100%, more preferably 90 to 100%. Therefore, even in such a high humidity environment, the genus Pseudomonas An applied voltage of at least 4 kV or more, more preferably 4.5 kV or more is required to generate positive and negative ions sufficient to sterilize the bacteria.

  In addition, the method for eradicating Pseudomonas spp. In a facility where the cultivation process is carried out by releasing positive and negative ions in the method for reducing growth failure of mushrooms of the present invention is also as described above. That is, an ion generator as described above is installed and operated in a facility where the cultivation process is carried out, thereby releasing positive ions and negative ions and sterilizing germs in the facility.

Here, the release of positive ions and negative ions in the facility where the cultivation process is performed is performed so that the amount of positive ions and negative ions in the facility is 2,000 / cm ³ or more, respectively. Is preferred. By setting it to 2,000 cells / cm ³ or more, sterilization of Pseudomonas spp. In the facility is made more effective, and mushroom growth disorders can be reduced more effectively. In addition, since it is not easy to measure the amount of positive and negative ions at every location in a facility under a high-humidity environment, in the present invention, preferably, a simulation of ion distribution is performed using a computer to determine the amount of ions in the facility. Calculate (pieces / cm ³ ).

  EXAMPLES Hereinafter, although an Example and a comparative example are given and this invention is demonstrated in detail, this invention is not limited to these.

<Example 1>
(Confirmation of sterilization effect of Pseudomonas sp.)
Prepare a closed processing box (65 cm x 40 cm x 30 cm) whose interior has been wiped with gauze containing an antistatic agent in advance, and place the ceramic dielectric in the closed processing box with the discharge electrode and dielectric electrode. An ion blower having an sandwiched structure and an ion generator that generates ions by applying an AC voltage between the discharge electrode and the dielectric electrode and an air blower (air flow 0.184 m ³ / min) was installed. This ion blower has the ability to generate an amount of ions (amount of positive and negative ions) of about 150,000 ions / cm ³ at a distance of 10 cm from the air outlet under conditions of a relative humidity of 40%. Furthermore, a plate agar medium (Pseudomonas selective medium) was installed on the leeward side at a position 45 cm away from the ion blower so as to be perpendicular to the blowing direction. The pre-preparation described above was performed, and first, a treatment test was performed. In the treatment test, Pseudomonas solution (108 / mL) was sprayed in a closed processing box, the ion blower (both ion generator and blower) in the closed treatment box was operated for 10 minutes, and the installed flat plate Pseudomonas spp. That fell in the air were collected using an agar medium. Next, a non-treatment test similar to the treatment test was performed except that only the blower was operated without operating the ion generator of the ion blower. Pseudomonas bacteria collected in each test were cultured at 22 ° C. for 48 hours. The same treatment test and no treatment test were performed twice in total. Table 3 shows the number of Pseudomonas species after culturing.

  As shown in Table 3, when the treatment test, that is, when the ion generator is operated, it can be seen that the number of Pseudomonas spp. Dropped in the air is significantly reduced. The decrease rate of Pseudomonas spp. Is 94.8% for the first time and 80% for the second time compared with the untreated test, and the sterilization effect is very high.

<Example 2>
(Cultivation of eringi using positive and negative ions)
According to the flowchart shown in FIG. 1, first, a medium was prepared by a commonly used means (step 101). Specifically, the medium composition was YK-E modified (Sugiogako: 290 g, Corn Cobmeal: 30 g, Rice bran: 45 g, Husuma: 45 g, Flour beet: 30 g, Shell fossil: 4 g, Nyokidale: 2 g / 1 bottle (fresh weight )), The water content was 65%. Next, 535 g of the prepared medium was filled in a polypropylene cultivation container having a capacity of 850 mL and a diameter of 58 mm (step 102), and 16 cultivation containers filled with medium were obtained. Next, after sterilizing by means usually used (step 103), each cultivation container was inoculated with an inoculum of “Elongi 12” (step 104). Subsequently, after culturing in a culture room for 20 days, aging was performed in the same room (step 105). The culture was performed in an environment at a temperature of 22 ° C. and a relative humidity of 70%, and aging was performed in an environment of a temperature of 22 ° C. and a relative humidity of 70%. Next, the bacteria were scraped by the scratching method (step 106). At this time, water was sprayed five times with a spray.

  Subsequently, the 16 cultivation containers subjected to the fungus scraping treatment were transferred to a growth room of 2.0 m × 3.5 m × 2.7 m shown in FIG. 3, and a sprouting step (step 107) and A growth step (step 109) was performed. Specifically, it is as follows.

FIG. 3 is a perspective view schematically showing the growth chamber used in this example. The growth chamber 301 has a size of 2.0 m (X direction) × 3.5 m (Y direction) × 2.7 m (Z direction), and two ion generators (not shown) at a position of Y = 0. ) Is installed (two fans 302 and 303, respectively). Although not shown, the height (Z direction) is 0.41 m, 1.25 m, and 1.7 m over X = 0 to 0.55 m and X = 1.55 to 2.0 m. Each has a shelf for placing cultivation containers. Each of the ion generators mounted on the blower 302 and the blower 303 has a structure in which a ceramic dielectric is sandwiched between a discharge electrode and a dielectric electrode, and under conditions of an applied voltage of 5.3 kV and a relative humidity of 40%, It has the ability to generate an amount of ions of about 100,000 ions / cm ³ (amount of positive and negative ions) at a distance of 10 cm from the blower outlet.

In the sprouting process (step 107), first, the above-mentioned 16 fungi are cultivated in the growth chamber 301 having the above-described structure maintained at a room temperature of 16.5 ° C. and a relative humidity of 90 to 95%. The container was moved, and the blower 302 and the blower 303 were operated almost simultaneously (the four ion generators were also operated in conjunction). The applied voltage to the ion generator was 5.3 to 5.7 kV. The air volume of the blower was 4 m ³ / min. Although not shown, louvers are attached to the positive and negative ion outlets of the blower 302 and the blower 303, the blower 303 blows out positive and negative ions in the X direction, and the blower 302 blows out positive and negative ions in the XZ oblique direction. . In addition, 16 cultivation containers put the container on the shelf provided in the growth room, and have arrange | positioned in it. Next, the sprouting process was completed by holding the blower 302 and the blower 303 for 13 days.

  Subsequently, the growth was performed in the growth chamber 301 at a temperature of 16 ° C. and a relative humidity of 90 to 95%, and the blower 302 and the blower 303 were continuously operated (step 109). During the growth process, the carbon dioxide gas concentration in the growth chamber 301 was kept at 1000 ppm, and illumination of 300 Lux was applied for 1 hour per day. The growing period was 4.0 days. The release of positive and negative ions stopped almost simultaneously with the end of the growth process. After completion of the growing process, harvesting was performed (step 110). The yield was 124.5 g on average per cultivation container.

  Here, when the number of bacteria in the growth chamber 301 was investigated, the number of bacteria falling in the air was 13 immediately before the operation of the blower 302 and the blower 303, but it was 0 after the growth process (step 109). Had decreased. The survey method is as follows.

  Regarding the number of bacteria falling in the air, first, petri dish medium (NA medium) was placed on the shelf surface (X = 0.45 m, Y = 1.75 m, Z = 1.25 m position) and floor surface (X = 0.45 m, Y = The bacteria were collected in the air by placing them one by one at a position of 1.75 m and Z = 0 m and opening for 15 minutes. Then, after culturing each at 25 ° C. for 48 hours, the generated colonies were counted, and these were averaged to obtain the number of bacteria falling in the air.

Furthermore, the ion distribution in the growth chamber 301 during the germination process (step 107) and the growth process (step 109) was calculated by simulation using a computer. FIG. 4 is a contour map showing the ion distribution in the growth chamber 301, FIG. 4 (a) is a contour map of the YZ plane at X = 0.45m, and FIG. 4 (b) is at X = 1.55m. It is a contour map of the YZ plane. The horizontal line in Fig.4 (a) and (b) shows that the shelf for putting a cultivation container in that position is provided (1.7m, 1.25m, 0.41m from the top, respectively). Each numerical value in FIGS. 4A and 4B represents the ion amount (number / cm ³ ) in a steady state at that position. The simulation uses “STREAM” (CFD analysis software of Software Cradle Co., Ltd.), the target space is 2.0 m × 3.5 m × 2.7 m, and the wind speed of the blower is about 4 m / sec, about 7 m. Steady state analysis was performed with an input boundary condition of 1 / sec.

From FIG. 4, it can be seen that the amount of each of the positive and negative ions is 2000 / cm ³ or more except in the very vicinity of the blower 401 and the blower 402 (corresponding to the blower 302 and the blower 303 in FIG. 3). .

<Example 3>
Sixteen cultivation containers subjected to the fungus scraping treatment (step 106) are transferred to the growth chamber 301 shown in FIG. 3, and positive and negative ions from 6 days before the germination process (step 107) is started until the end of the growth process (step 109). Eringi was cultivated and harvested in the same manner as in Example 2 except that it was released. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

  Here, when the number of bacteria in the growth chamber 301 was investigated, the number of bacteria falling in the air was 5 at the start of the budding process (step 107), but decreased to 0 after the growth process (step 109). It was decreasing.

<Comparative Example 1>
In the growth chamber 301 shown in FIG. 3, instead of installing the blower 302 and the blower 303 equipped with an ion generator, a titanium oxide photocatalytic sterilizer (Fresh Long (registered trademark) PCE-101 manufactured by Nippon Inorganic Co., Ltd.) is used. One unit was installed, and eringi was cultivated and harvested in the same manner as in Example 2 except that it was operated at the start of the budding process (step 107). Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

  Here, when the number of bacteria in the growth chamber 301 was examined, the number of bacteria falling in the air was 92 at the start of the budding process (step 107), but now 12 after the growth process (step 109). It was decreasing.

<Comparative example 2>
In the growth chamber 301 shown in FIG. 3, eringi was cultivated and harvested in the same manner as in Example 2 except that no device for sterilizing the germs in the growth chamber 301 was provided. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Example 4>
The eringi was cultivated and harvested in the same manner as in Example 2 except that the inoculated inoculum was an inoculum of eringi “long fungus 15”. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Example 5>
Eringi was cultivated and harvested in the same manner as in Example 3 except that the inoculated inoculum was an inoculum of Eringi "Nagae No. 15". Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Comparative Example 3>
Eringi was cultivated and harvested in the same manner as in Comparative Example 1 except that the inoculated inoculum was an inoculum of Eringi "Nagae No. 15". Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Comparative Example 4>
Eringi was cultivated and harvested in the same manner as in Comparative Example 2 except that the inoculated inoculum was an inoculum of Eringi "Nagae No. 15". Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Example 6>
Eringi was cultivated and harvested in the same manner as in Example 2 except that the inoculated inoculum was an inoculum of Katsuragi Sangyo Eringi “KE106”. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Example 7>
Eringi was cultivated and harvested in the same manner as in Example 3 except that the inoculated inoculum was an inoculum of Katsuragi Sangyo Co., Ltd. “KE106”. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Comparative Example 5>
Eringi was cultivated and harvested in the same manner as in Comparative Example 1 except that the inoculated inoculum was an inoculum of Katsuragi Sangyo Eringi “KE106”. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

<Comparative Example 6>
Eringi was cultivated and harvested in the same manner as in Comparative Example 2 except that the inoculated inoculum was an inoculum of Katsuragi Sangyo Eringi “KE106”. Table 4 shows the number of days of sprouting process, the number of days of growing process, and the yield of eringi.

  Table 4 shows the quality of the eringi obtained in Examples 2 to 7 and Comparative Examples 1 to 6, the degree of withering symptoms, and the control value.

Here, the “average yield (g)” in Table 4 is an average value of the yield of 16 harvested eringi. “Yield ratio” is the average yield when the average yield of eringi obtained by the conventional cultivation method (Comparative Example 2, Comparative Example 4 and Comparative Example 6) in which no sterilization apparatus is provided in the facility is defined as 100. . The “quality” of the eringi is a value obtained by visually determining the yield, the irregular shape of the fungus, the twist of the fungus pattern, the occurrence of the flapping, and the degree of the withering symptom, and calculating the average. The numerical value is based on the following criteria.
1: No = Yield is less than 100 g, and abnormal malformations and pests are observed in the fruit umbrella and fungus of the fruiting body.
2: Slightly no intermediate between 1 and 3.
3: Medium = yield is 100 g or more, and malformations and pests appear on the fungus and fungus of the fruit body.
4: Slightly good = intermediate between 3 and 5.
5: Good = yield is 130 g or more, and there are no malformations and pests in the fungus umbrella and fungus of the fruit body.

  The “degree of withering symptom occurrence” was investigated for the presence or absence of withering symptom for each of the 16 strains, and the degree of occurrence was classified into 6 levels (from 0 to 5 in order from the lightest occurrence) to It is a numerical value obtained by fitting.

Occurrence of withering symptoms = {Σ (degree of occurrence × number of strains)} / (number of strains investigated) (1)
In addition, the generation | occurrence | production degree 0-5 said the following symptoms, respectively, and judged visually. Here, the malformation means that the fungus pattern (stem) is bent or raised and the umbrella is irregularly shaped.
0: No occurrence.
1: Discoloration is seen in a part of fungus umbrella and fungus pattern.
2: Discoloration / deformation is observed in about 1/3 of the stock.
3: Discoloration / deformation is seen in about half of the stock.
4: Discoloration / deformation is observed in about 2/3 of the stock.
5: The entire strain is discolored / shrinked, leading to death.

  “Control value” (%) means how much withering symptoms occur with respect to the degree of withering symptoms in the conventional cultivation methods (Comparative Example 2, Comparative Example 4 and Comparative Example 6) in which no sterilization apparatus is provided in the facility. It is a quantification of whether the degree could be reduced. That is, the “control value” (%) is represented by the following formula (2).

Control value (%) = 100 − {(degree of occurrence of withering symptoms) / (degree of occurrence of withering symptoms in Comparative Example 2, Comparative Example 4 or Comparative Example 6)} × 100 (2)
As shown in Table 4, in all cultivars, the quality of eringi was greatly improved by the cultivation method according to the present invention. Moreover, in the case of long bacteria 12 and long bacteria 15, even if compared with the cultivation method (Comparative Example 2, Comparative Example 4 and Comparative Example 6) using the photocatalytic sterilization apparatus, the cultivation method according to the present invention is used. , The quality of eringi improved. The degree of withering symptom occurrence is significantly reduced in all cultivars, and in particular, in the long bacteria No. 12 and the long bacteria No. 15, a cultivation method using a photocatalytic disinfection device (Comparative Example 2, Comparative Example 4 and A reduction effect superior to that of Comparative Example 6) was observed. This is apparent from the numerical value of the control value.

  In the following examples and comparative examples, eringi was cultivated in an actual producer facility, and the sterilization effect and the withering symptom reduction effect by releasing positive and negative ions were confirmed.

<Example 8>
After obtaining 32 culture containers filled with medium, sterilize by means usually used, except inoculating each culture container with the inoculum of Nagano Prefectural Institute of Rural Engineering Eringi E-3 “Erinco” It carried out similarly to Example 2 to step 106 (bacteria scraping process). Next, 32 cultivation containers were moved to the sprout room, and a sprout process was performed (step 107). Next, the 32 cultivated containers that had been sprouting were transferred to a leveling room (7.2 m × 5.4 m × 2.7 m), and the leveling process was performed for 3 days (step 108). The conditioned room temperature was 16 ° C. and the relative humidity was 95-100%. FIG. 5 is a top view showing the structure of the leveling room. As shown in FIG. 5, two fans 501 and 502, which are the same as those in Example 2, and equipped with two ion generators, were installed at the position shown in FIG. 5. As shown in FIG. 5, the 32 cultivation containers were placed on a shelf having a height of 80 cm provided in the leveling room and at a position 503 corresponding to the center of the leveling room. Here, positive / negative ion release in the run-in room was performed one week before the start of the run-in process, and was continuously operated until the end of the run-in process.

  Thus, in this embodiment, positive and negative ions are released only in the leveling process. Table 5 shows the number of bacteria falling in the air and the number of airborne bacteria immediately before the blower in the run-in room, and the number of bacteria falling in the air and the number of airborne bacteria at the start of the smoothing process one week later.

  As shown in Table 5, both the filamentous fungi and the bacteria were found to have decreased in number due to the operation of the blower for one week. The method for investigating the number of filamentous fungi and the number of bacteria is the same as in Example 2 except that the culture time of the filamentous fungus is 72 hours.

  Next, 32 cultivation containers which finished the leveling process were moved to the growth room, and the growth process was performed (step 109). The temperature in the growth chamber was 16 ° C. and the relative humidity was 90%. During the growth process, the carbon dioxide concentration was controlled to 1500 to 2000 ppm, and 150 Lux was applied for 12 hours a day. The growing period was 16 days. After completion of the growing process, harvesting was performed (step 110). The yield was 151.8 g on average per cultivation container.

<Example 9>
Eringi was cultivated and harvested in the same manner as in Example 8 except that the release of positive and negative ions in the conditioned room was performed 2 weeks before the start of the conditioned process. Table 5 shows the number of bacteria falling in the air and the number of airborne bacteria after 2 weeks of running-in air blower operation.

<Comparative Example 7>
Eringi was cultivated and harvested in the same manner as in Example 8 except that no apparatus for sterilizing indoor germs was provided.

Table 6 shows the yield, quality, degree of coloration, degree of withering and the control value of eringi obtained in Examples 8 and 9 and Comparative Example 7. In addition, "the coloring generation degree" is visually judged as 甚: 5 to fine: 1, and the average is calculated. The numerical criteria are as follows.
0: No occurrence.
1: Colored part of fungus umbrella stem.
2: Colored 1/3 of the fungus stem.
3: Colored on half of the fungus stem.
4: Colored 2/3 of the fungus stem.
5: The entire fungus stem is colored.

  As shown in Table 6, it was found that the release of positive and negative ions improves the quality of eringi. It was also found that the release of positive and negative ions can significantly reduce the withering symptoms and the coloring of fruiting bodies.

  It should be understood that the embodiments and examples disclosed herein are illustrative and non-restrictive in every respect. The scope of the present invention is defined by the terms of the claims, rather than the description above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.

It is a flowchart which shows roughly a preferable example of the cultivation method of the mushroom of this invention. It is the schematic apparatus used in order to measure the applied voltage which can be discharged shown by Table 1. FIG. It is a perspective view which shows roughly the growth chamber used in Example 2. FIG. It is a contour map which shows ion distribution of the growth chamber shown by FIG. 3, (a) is a contour map of the YZ plane in X = 0.45m, (b) is a contour map of the YZ plane in X = 1.55m. It is. It is a top view which shows the structure of the leveling room used in Example 8.

Explanation of symbols

  201 acrylic box, 202 ion generator, 203 blower, 204 ion measuring device, 301 growth room, 302, 303, 401, 402, 501, 502 blower.

Claims (11)

In the cultivation method of mushrooms including the cultivation process of growing the primordium after the formation of the primordium,
A method for cultivating mushrooms, wherein positive ions and negative ions are released in a facility where the cultivation step is carried out for a certain period of time during the cultivation step to eliminate germs in the facility.   The cultivation process includes a sprouting process for forming a primordium and a growing process for growing the primordium, and releases positive ions and negative ions into the facility where the sprouting process and / or the growing process is performed. The cultivation method of the mushroom of Claim 1 characterized by the above-mentioned.   The cultivation process includes a sprouting process for forming a primordial base, a leveling process for aligning the primordial base, and a growth process for growing the primordial base. In the facility where the leveling process is performed, positive ions and The method for cultivating mushrooms according to claim 1, wherein negative ions are released. Cultivation of mushrooms according to any one of claims 1 to 3, wherein positive ions and negative ions are released so that the amount of positive ions and negative ions in the facility is 2000 pieces / cm ³ or more, respectively. Method.   5. The mushroom cultivation method according to claim 1, wherein the relative humidity in the facility is in the range of 80 to 100%.   The mushroom cultivation method according to any one of claims 1 to 5, wherein the miscellaneous bacteria are Pseudomonas species.   After the formation of the primordium, Pseudomonas sp. Is eliminated by releasing positive ions and negative ions for a certain period of time during the cultivation process in the facility where the cultivation process for growing the primordial is carried out. A method for reducing growth failure of mushrooms characterized by fungi.   The cultivation process includes a sprouting process for forming a primordium and a growing process for growing the primordium, and releases positive ions and negative ions into the facility where the sprouting process and / or the growing process is performed. The method for reducing a growth disorder of a mushroom according to claim 7.   The cultivation process includes a sprouting process for forming a primordial base, a leveling process for aligning the primordial base, and a growth process for growing the primordial base. In the facility where the leveling process is performed, positive ions and The method for reducing mushroom growth disorder according to claim 7, wherein negative ions are released. The growth of mushrooms according to any one of claims 7 to 9, wherein positive ions and negative ions are released so that the amount of positive ions and negative ions in the facility is 2000 / cm ³ or more, respectively. How to reduce obstacles.   The relative humidity in a facility is in the range of 80 to 100%, and the method for reducing mushroom growth disorder according to any one of claims 7 to 10.

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